CL2017000892A1 - Métodos de cultivo por lotes alimentados con repetición - Google Patents

Métodos de cultivo por lotes alimentados con repetición

Info

Publication number
CL2017000892A1
CL2017000892A1 CL2017000892A CL2017000892A CL2017000892A1 CL 2017000892 A1 CL2017000892 A1 CL 2017000892A1 CL 2017000892 A CL2017000892 A CL 2017000892A CL 2017000892 A CL2017000892 A CL 2017000892A CL 2017000892 A1 CL2017000892 A1 CL 2017000892A1
Authority
CL
Chile
Prior art keywords
volume
batch
microorganisms
methods
culture
Prior art date
Application number
CL2017000892A
Other languages
English (en)
Inventor
Zhiyong Sun
Michael Milway
Kevin Berryman
Mercia Valentine
Roberto E Armenta
Original Assignee
Mara Renewables Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mara Renewables Corp filed Critical Mara Renewables Corp
Publication of CL2017000892A1 publication Critical patent/CL2017000892A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/10—Protozoa; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Cultivation Of Plants (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

<p>EN LA PRESENTE SE PROPORCIONAN MÉTODOS DE CULTIVO DE UN MICROORGANISMO. EL MÉTODO INCLUYE PROPORCIONAR UN RECIPIENTE QUE COMPRENDA UNO O MÁS MICROORGANISMOS Y MEDIO, DONDE LOS MICROORGANISMOS Y EL MEDIO FORMAN UN VOLUMEN INICIAL. LOS MICROORGANISMOS Y EL MEDIO SE CULTIVAN HASTA QUE EL CULTIVO ALCANCE UN INDICADOR UMBRAL, DONDE CULTIVAR COMPRENDE ALIMENTAR EL CULTIVO CON UNA O MÁS FUENTES DE CARBONO Y DONDE EL CULTIVO SE ENCUENTRA EN UN VOLUMEN UMBRAL CUANDO SE ALCANZA EL INDICADOR UMBRAL. EL MÉTODO TAMBIÉN INCLUYE RECOLECTAR UNA PARTE DEL VOLUMEN UMBRAL PARA DEJAR UN VOLUMEN RESIDUAL QUE CONSTITUYA 40 % O MENOS DEL VOLUMEN INICIAL Y AGREGAR MEDIO FRESCO AL RECIPIENTE EN UNA CANTIDAD PARA REGRESAR EL VOLUMEN DEL CULTIVO AL VOLUMEN INICIAL.</p>
CL2017000892A 2014-10-16 2017-04-11 Métodos de cultivo por lotes alimentados con repetición CL2017000892A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201462064694P 2014-10-16 2014-10-16

Publications (1)

Publication Number Publication Date
CL2017000892A1 true CL2017000892A1 (es) 2017-11-03

Family

ID=55746208

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2017000892A CL2017000892A1 (es) 2014-10-16 2017-04-11 Métodos de cultivo por lotes alimentados con repetición

Country Status (14)

Country Link
US (3) US10920261B2 (es)
EP (1) EP3207165B1 (es)
JP (1) JP6773301B2 (es)
KR (1) KR102501032B1 (es)
CN (2) CN112961785A (es)
AU (2) AU2015332095B2 (es)
BR (1) BR112017003558B1 (es)
CA (1) CA2960450C (es)
CL (1) CL2017000892A1 (es)
DK (1) DK3207165T3 (es)
ES (1) ES2785331T3 (es)
IL (1) IL250269A0 (es)
MX (1) MX383120B (es)
WO (1) WO2016059541A1 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12410458B2 (en) 2014-10-16 2025-09-09 MARA Renewables Corporation Semi-continuous culture methods

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109136308B (zh) * 2018-09-26 2021-01-26 武汉中科光谷绿色生物技术有限公司 一种提高发酵产聚唾液酸的方法及发酵液
CN109161570B (zh) * 2018-09-26 2020-12-18 武汉中科光谷绿色生物技术有限公司 一种提高发酵产n-乙酰神经氨酸的方法及发酵液

Family Cites Families (13)

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US5340594A (en) 1988-09-07 1994-08-23 Omegatech Inc. Food product having high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
EP2960325B1 (en) * 2000-01-28 2017-09-27 DSM IP Assets B.V. Enhanced production of lipids containing polyenoic fatty acids by high density cultures of eukaryotic microbes in fermentors
WO2005021735A1 (en) * 2003-09-01 2005-03-10 Novozymes A/S Method for increasing yield of biomass of and/or components of biomass from marine microorganisms
ES2626018T3 (es) * 2005-06-07 2017-07-21 Dsm Nutritional Products Ag Microorganismos eucariotas para la producción de lípidos y antioxidantes
CN101981201A (zh) * 2006-08-01 2011-02-23 加拿大海洋营养食品有限公司 产油微生物及改良这些微生物的方法
US20100124583A1 (en) * 2008-04-30 2010-05-20 Xyleco, Inc. Processing biomass
EP2071019A1 (en) * 2007-12-15 2009-06-17 Lonza AG Method for the cultivation of microoranisms of the order thraustochytriales
EP2883958B1 (en) 2009-02-25 2018-04-11 V.B.Medicare Pvt. Ltd. Improved methods for fermentative production of docosahexaenoic acid
EP2456855B1 (en) * 2009-07-23 2017-03-29 GE Healthcare Bio-Sciences Corp. Drain down and re-feed of microcarrier bioreactor
WO2012120375A1 (en) * 2011-03-07 2012-09-13 Ocean Nutrition Canada Limited Engineering thraustochytrid microorganisms
IN2014DN07894A (es) * 2012-03-26 2015-04-24 Kansai Chem Eng
CN103436561B (zh) * 2013-09-05 2014-11-26 江南大学 以棉纤维材料固定琥珀酸放线杆菌发酵生产丁二酸的方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12410458B2 (en) 2014-10-16 2025-09-09 MARA Renewables Corporation Semi-continuous culture methods

Also Published As

Publication number Publication date
EP3207165A1 (en) 2017-08-23
BR112017003558B1 (pt) 2023-10-03
CA2960450A1 (en) 2016-04-21
IL250269A0 (en) 2017-03-30
MX2017002150A (es) 2017-08-21
NZ728642A (en) 2024-07-05
US20160108453A1 (en) 2016-04-21
BR112017003558A2 (pt) 2018-02-27
JP6773301B2 (ja) 2020-10-21
ES2785331T3 (es) 2020-10-06
US20210123083A1 (en) 2021-04-29
MX383120B (es) 2025-03-11
AU2021202832A1 (en) 2021-06-03
EP3207165A4 (en) 2018-06-06
DK3207165T3 (da) 2020-04-06
AU2021202832B2 (en) 2023-08-24
CN112961785A (zh) 2021-06-15
US10920261B2 (en) 2021-02-16
EP3207165B1 (en) 2020-02-05
WO2016059541A1 (en) 2016-04-21
CN106604998B (zh) 2021-03-30
AU2015332095A1 (en) 2017-02-16
US11827918B2 (en) 2023-11-28
CN106604998A (zh) 2017-04-26
KR20170067748A (ko) 2017-06-16
US20240060112A1 (en) 2024-02-22
KR102501032B1 (ko) 2023-02-16
CA2960450C (en) 2023-02-14
JP2017532022A (ja) 2017-11-02
AU2015332095B2 (en) 2021-02-18

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