CL2019000540A1 - Método para aislar células madre mesenquimales a partir de membrana amniótica de cordón umbilical utilizando un medio de cultivo celular resumen de la invención. - Google Patents
Método para aislar células madre mesenquimales a partir de membrana amniótica de cordón umbilical utilizando un medio de cultivo celular resumen de la invención.Info
- Publication number
- CL2019000540A1 CL2019000540A1 CL2019000540A CL2019000540A CL2019000540A1 CL 2019000540 A1 CL2019000540 A1 CL 2019000540A1 CL 2019000540 A CL2019000540 A CL 2019000540A CL 2019000540 A CL2019000540 A CL 2019000540A CL 2019000540 A1 CL2019000540 A1 CL 2019000540A1
- Authority
- CL
- Chile
- Prior art keywords
- umbilical cord
- amniotic membrane
- culture medium
- stem cells
- cell culture
- Prior art date
Links
- 210000003954 umbilical cord Anatomy 0.000 title abstract 4
- 210000001691 amnion Anatomy 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 239000006143 cell culture medium Substances 0.000 title 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 title 1
- 210000000130 stem cell Anatomy 0.000 abstract 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract 2
- 102000003729 Neprilysin Human genes 0.000 abstract 2
- 108090000028 Neprilysin Proteins 0.000 abstract 2
- 239000012091 fetal bovine serum Substances 0.000 abstract 2
- 102000006354 HLA-DR Antigens Human genes 0.000 abstract 1
- 108010058597 HLA-DR Antigens Proteins 0.000 abstract 1
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 abstract 1
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 abstract 1
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 abstract 1
- 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 abstract 1
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 abstract 1
- 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000008194 pharmaceutical composition Substances 0.000 abstract 1
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- C—CHEMISTRY; METALLURGY
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
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- C12N2500/00—Specific components of cell culture medium
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- C12N2500/00—Specific components of cell culture medium
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- C12N2500/00—Specific components of cell culture medium
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- C12N2500/34—Sugars
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- C12N2500/00—Specific components of cell culture medium
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
- C12N2506/025—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells from extra-embryonic cells, e.g. trophoblast, placenta
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- Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
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- Immunology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
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- Rheumatology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
LA PRESENTE INVENCIÓN SE REFIERE A UN MÉTODO PARA AISLAR UNA POBLACIÓN DE CÉLULAS MADRE MESENQUIMALES DE LA MEMBRANA AMNIÓTICA DEL CORDÓN UMBILICAL, EL MÉTODO QUE COMPRENDE CULTIVAR EL TEJIDO DE CORDÓN UMBILICAL EN UN MEDIO DE CULTIVO QUE COMPRENDE DMEM (MEDIO EAGLE MODIFICADO DE DULBECCO), F12 (MEDIO F12 DE HAM), M171 (MEDIO 171) Y FBS (SUERO BOVINO FETAL). LA INVENCIÓN TAMBIÉN SE REFIERE A UNA POBLACIÓN DE CÉLULAS MADRE MESENQUIMALES AISLADAS DE LA MEMBRANA AMNIÓTICA DEL CORDÓN UMBILICAL, EN DONDE AL MENOS APROXIMADAMENTE 90% O MÁS CÉLULAS DE LA POBLACIÓN DE CÉLULAS MADRE EXPRESAN CADA UNO DE LOS SIGUIENTES MARCADORES: CD73, CD90 Y CD105 Y CARECEN DE EXPRESIÓN DE LOS SIGUIENTES MARCADORES: CD34, CD45 Y HLA-DR. LA INVENCIÓN TAMBIÉN SE REFIERE A UNA COMPOSICIÓN FARMACÉUTICA DE ESTA POBLACIÓN DE CÉLULAS MADRE MESENQUIMALES.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662404582P | 2016-10-05 | 2016-10-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CL2019000540A1 true CL2019000540A1 (es) | 2019-08-09 |
Family
ID=61832065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CL2019000540A CL2019000540A1 (es) | 2016-10-05 | 2019-02-28 | Método para aislar células madre mesenquimales a partir de membrana amniótica de cordón umbilical utilizando un medio de cultivo celular resumen de la invención. |
Country Status (21)
| Country | Link |
|---|---|
| US (4) | US10988736B2 (es) |
| EP (1) | EP3523421B1 (es) |
| JP (3) | JP7262775B2 (es) |
| KR (1) | KR102491558B1 (es) |
| CN (1) | CN109804063B (es) |
| AU (2) | AU2017339344B2 (es) |
| CA (1) | CA3031978A1 (es) |
| CL (1) | CL2019000540A1 (es) |
| CO (1) | CO2019002569A2 (es) |
| ES (1) | ES3009517T3 (es) |
| HU (1) | HUE070873T2 (es) |
| IL (2) | IL264197B2 (es) |
| MX (1) | MX2019003936A (es) |
| MY (1) | MY202675A (es) |
| PH (1) | PH12019500187A1 (es) |
| PL (1) | PL3523421T3 (es) |
| SA (1) | SA519401455B1 (es) |
| SG (1) | SG11201900753VA (es) |
| TW (2) | TWI836505B (es) |
| WO (1) | WO2018067071A1 (es) |
| ZA (1) | ZA201900207B (es) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2999420A1 (en) | 2005-09-27 | 2007-04-05 | Tissuetech, Inc. | Amniotic membrane preparations and purified compositions and methods of use |
| WO2012149486A1 (en) | 2011-04-28 | 2012-11-01 | Tissuetech, Inc. | Methods of modulating bone remodeling |
| EP3838293A1 (en) | 2012-07-11 | 2021-06-23 | Tissuetech, Inc. | Compositions containing hc-ha/ptx3 complexes and methods of use thereof |
| WO2016138025A2 (en) | 2015-02-23 | 2016-09-01 | Tissuetech, Inc. | Apparatuses and methods for treating ophthalmic diseases and disorders |
| TWI720984B (zh) | 2015-05-20 | 2021-03-11 | 美商帝聖工業公司 | 用於防止上皮細胞增生及上皮-間質轉移之組合物及方法 |
| TW201733600A (zh) | 2016-01-29 | 2017-10-01 | 帝聖工業公司 | 胎兒扶持組織物及使用方法 |
| PL3523421T3 (pl) * | 2016-10-05 | 2025-06-09 | Cellresearch Corporation Pte. Ltd. | Sposób izolowania mezenchymalnych komórek macierzystych z błony owodniowej pępowiny przy użyciu podłoża do hodowli komórkowej |
| KR102015502B1 (ko) * | 2018-03-29 | 2019-08-28 | 아키소스템바이오스트래티지스(주) | 인간 제대로부터 줄기세포를 분리하는 방법 |
| WO2019199230A1 (en) * | 2018-04-09 | 2019-10-17 | Cellresearch Corporation Pte. Ltd. | A method of transporting mesenchymal stem cells by means of a cell culture medium and a method of administering stem cells to wounds |
| EP3772936A4 (en) * | 2018-04-09 | 2022-01-05 | CellResearch Corporation Pte. Ltd. | METHOD FOR TRANSPORTING MESENCHYMAL STEM CELLS BY A TRANSPORT SOLUTION AND METHOD FOR ADMINISTERING STEM CELLS TO WOUNDS |
| US12460178B2 (en) | 2018-04-12 | 2025-11-04 | Cellresearch Corporation Pte. Ltd. | Method of inducing or improving wound healing properties of mesenchymal stem cells |
| TWI882956B (zh) * | 2018-04-12 | 2025-05-11 | 新加坡商細胞研究私人有限公司 | 一種誘導或改善間質幹細胞傷口癒合特性的方法 |
| US20210324335A1 (en) * | 2018-09-04 | 2021-10-21 | Academia Sinica | Medium composition and method for culturing mesenchymal stem cells |
| CN113015535A (zh) * | 2018-10-05 | 2021-06-22 | 得克萨斯大学体系董事会 | 扩增间充质基质细胞的方法 |
| AU2020348295A1 (en) | 2019-09-16 | 2022-04-07 | Insmed Incorporated | Anti-stem cell factor antibodies and methods of use thereof |
| WO2021071430A1 (en) * | 2019-10-08 | 2021-04-15 | Cellresearch Corporation Pte. Ltd. | A mesenchymal stem cell storing or transport formulation and methods of making and using the same |
| AU2020362379A1 (en) | 2019-10-08 | 2022-04-07 | Cellresearch Corporation Pte. Ltd. | A method of assessing wound healing potency of a mesenchymal stem population and related methods of selecting mesenchymal stem cells and identifying tissue as starting material for producing a mesenchymal stem cell population |
| JP2022108741A (ja) * | 2021-01-13 | 2022-07-26 | 株式会社カネカ | 羊膜由来接着性幹細胞の製造方法 |
| JP7725119B2 (ja) * | 2023-01-27 | 2025-08-19 | 株式会社Rainbow | 自動培養装置における細胞増殖制御方法 |
| CN118718106A (zh) * | 2024-08-30 | 2024-10-01 | 中科合肥技术创新工程院 | 一种基于多能干细胞的组织工程皮肤及其制备方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL1789534T3 (pl) * | 2004-08-16 | 2015-03-31 | Cellresearch Corp Pte Ltd | Wyodrębnienie komórek macierzystych/progenitorowych z błony owodniowej pępowiny |
| CN103555655B (zh) * | 2005-10-21 | 2018-02-27 | 细胞研究私人有限公司 | 自脐带羊膜分离和培养干/祖细胞及其分化的细胞的应用 |
| CN101525594B (zh) * | 2009-04-17 | 2011-04-20 | 中国医学科学院血液学研究所 | 一种培养间充质干细胞的低血清浓度完全培养基和利用该培养基培养间充质干细胞的方法 |
| JP2013514072A (ja) * | 2009-12-18 | 2013-04-25 | シー．ビー．ビー．ライフライン バイオテク リミテッド | 臍帯組織から間葉系前駆細胞の亜集団を含む単核細胞および内皮前駆細胞の亜集団を含む血管細胞を単離する方法 |
| CN103266081B (zh) * | 2012-01-21 | 2015-05-13 | 中国人民解放军军事医学科学院附属医院 | 从脐带中分离培养间充质干细胞的高效方法 |
| US8940294B2 (en) * | 2012-03-02 | 2015-01-27 | Tissuetech, Inc. | Methods of isolating and culturing stem cells |
| CN103013917A (zh) * | 2012-11-30 | 2013-04-03 | 陆华 | 人羊膜间充质干细胞分化为神经元样细胞的诱导方法 |
| CA2901653C (en) * | 2013-03-01 | 2019-01-08 | Gunther Eissner | Protection of the vascular endothelium from immunologically mediated cytotoxic reactions with human cd34-negative progenitor cells |
| JP6745449B2 (ja) * | 2013-12-27 | 2020-08-26 | ゼノアックリソース株式会社 | 臍帯組織の凍結保存方法 |
| CN105420184A (zh) * | 2015-12-11 | 2016-03-23 | 郭镭 | 一种从脐带外层羊膜组织中分离培养脐带间充质干细胞的方法 |
| CN105420179A (zh) * | 2015-12-17 | 2016-03-23 | 斯坦姆（天津）生物技术研究有限公司 | 一种从脐带和胎盘羊膜组织同时提取上皮细胞和间充质干细胞的方法 |
| PL3523421T3 (pl) * | 2016-10-05 | 2025-06-09 | Cellresearch Corporation Pte. Ltd. | Sposób izolowania mezenchymalnych komórek macierzystych z błony owodniowej pępowiny przy użyciu podłoża do hodowli komórkowej |
| TW202002999A (zh) * | 2018-04-06 | 2020-01-16 | 新加坡商細胞研究私人有限公司 | 臍帶羊膜的基本上純的間質幹細胞群用於產生攜帶轉殖基因的哺乳動物幹細胞的用途 |
| WO2021071430A1 (en) * | 2019-10-08 | 2021-04-15 | Cellresearch Corporation Pte. Ltd. | A mesenchymal stem cell storing or transport formulation and methods of making and using the same |
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2017
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- 2017-10-05 MY MYPI2019000972A patent/MY202675A/en unknown
- 2017-10-05 SG SG11201900753VA patent/SG11201900753VA/en unknown
- 2017-10-05 CA CA3031978A patent/CA3031978A1/en active Pending
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- 2017-10-05 KR KR1020197004328A patent/KR102491558B1/ko active Active
- 2017-10-05 WO PCT/SG2017/050500 patent/WO2018067071A1/en not_active Ceased
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- 2017-10-05 TW TW111125698A patent/TWI836505B/zh active
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- 2017-10-05 JP JP2019515832A patent/JP7262775B2/ja active Active
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- 2017-10-05 EP EP17858825.7A patent/EP3523421B1/en active Active
- 2017-10-05 AU AU2017339344A patent/AU2017339344B2/en active Active
- 2017-10-05 IL IL307825A patent/IL307825B2/en unknown
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2019
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- 2019-01-25 PH PH12019500187A patent/PH12019500187A1/en unknown
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2021
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2022
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2023
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2025
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- 2025-06-03 US US19/227,355 patent/US20250290043A1/en active Pending
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