CL2021001409A1 - Sistemas y métodos para facilitar la selección de clones - Google Patents
Sistemas y métodos para facilitar la selección de clonesInfo
- Publication number
- CL2021001409A1 CL2021001409A1 CL2021001409A CL2021001409A CL2021001409A1 CL 2021001409 A1 CL2021001409 A1 CL 2021001409A1 CL 2021001409 A CL2021001409 A CL 2021001409A CL 2021001409 A CL2021001409 A CL 2021001409A CL 2021001409 A1 CL2021001409 A1 CL 2021001409A1
- Authority
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- Prior art keywords
- image
- processor
- colony
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- cell
- Prior art date
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V20/00—Scenes; Scene-specific elements
- G06V20/60—Type of objects
- G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
- G06V20/695—Preprocessing, e.g. image segmentation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F18/00—Pattern recognition
- G06F18/20—Analysing
- G06F18/24—Classification techniques
- G06F18/241—Classification techniques relating to the classification model, e.g. parametric or non-parametric approaches
- G06F18/2413—Classification techniques relating to the classification model, e.g. parametric or non-parametric approaches based on distances to training or reference patterns
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06N—COMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
- G06N3/00—Computing arrangements based on biological models
- G06N3/02—Neural networks
- G06N3/04—Architecture, e.g. interconnection topology
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06N—COMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
- G06N3/00—Computing arrangements based on biological models
- G06N3/02—Neural networks
- G06N3/04—Architecture, e.g. interconnection topology
- G06N3/0464—Convolutional networks [CNN, ConvNet]
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06N—COMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
- G06N3/00—Computing arrangements based on biological models
- G06N3/02—Neural networks
- G06N3/08—Learning methods
- G06N3/09—Supervised learning
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V10/00—Arrangements for image or video recognition or understanding
- G06V10/70—Arrangements for image or video recognition or understanding using pattern recognition or machine learning
- G06V10/764—Arrangements for image or video recognition or understanding using pattern recognition or machine learning using classification, e.g. of video objects
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V10/00—Arrangements for image or video recognition or understanding
- G06V10/70—Arrangements for image or video recognition or understanding using pattern recognition or machine learning
- G06V10/82—Arrangements for image or video recognition or understanding using pattern recognition or machine learning using neural networks
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V20/00—Scenes; Scene-specific elements
- G06V20/60—Type of objects
- G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
- G06V20/693—Acquisition
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V20/00—Scenes; Scene-specific elements
- G06V20/60—Type of objects
- G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
- G06V20/698—Matching; Classification
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- Engineering & Computer Science (AREA)
- Theoretical Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Evolutionary Computation (AREA)
- Multimedia (AREA)
- Molecular Biology (AREA)
- Artificial Intelligence (AREA)
- Software Systems (AREA)
- Computing Systems (AREA)
- Data Mining & Analysis (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medical Informatics (AREA)
- Databases & Information Systems (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Computational Linguistics (AREA)
- Biophysics (AREA)
- Mathematical Physics (AREA)
- Evolutionary Biology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Food Science & Technology (AREA)
Abstract
Un método para facilitar la selección de clones incluye generar imágenes de secuencia de tiempo de un pocillo que contiene un medio, que incluyen una primera imagen y una segunda imagen posterior. El método también incluye detectar, por uno o más procesadores que analizan la primera imagen, uno o más objetos candidatos representados en la primera imagen y, para cada uno de los objetos candidatos, determinar si el objeto es una célula individual analizando una imagen del objeto usando una red neuronal convolucional. El método incluye adicionalmente detectar, analizando la segunda imagen con el procesador o procesadores, una colonia de células representada en la segunda imagen, y determinar, por el procesador o procesadores, si la colonia se formó a partir de únicamente una célula basándose al menos en si se determinó que cada objeto candidato era una célula individual. El método incluye adicionalmente generar, por el procesador o procesadores, datos de salida que indican si la colonia se formó a partir de únicamente una célula.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862774154P | 2018-11-30 | 2018-11-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CL2021001409A1 true CL2021001409A1 (es) | 2021-11-12 |
Family
ID=68966014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CL2021001409A CL2021001409A1 (es) | 2018-11-30 | 2021-05-28 | Sistemas y métodos para facilitar la selección de clones |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US12561992B2 (es) |
| EP (1) | EP3888004B1 (es) |
| JP (2) | JP7789560B2 (es) |
| KR (1) | KR20210099028A (es) |
| CN (1) | CN113168519A (es) |
| AU (1) | AU2019387079B2 (es) |
| BR (1) | BR112021010404A2 (es) |
| CA (1) | CA3120281A1 (es) |
| CL (1) | CL2021001409A1 (es) |
| EA (1) | EA202191499A1 (es) |
| IL (1) | IL283577B2 (es) |
| MX (1) | MX2021005938A (es) |
| SG (1) | SG11202105399RA (es) |
| WO (1) | WO2020112723A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20220073738A (ko) * | 2019-08-30 | 2022-06-03 | 주노 쎄러퓨티크스 인코퍼레이티드 | 세포 분류를 위한 기계 학습 방법 |
| EP4115388A1 (en) | 2020-03-06 | 2023-01-11 | Amgen Inc. | Systems and methods for assessing cell growth rates |
| US12183119B2 (en) * | 2021-04-12 | 2024-12-31 | Bitsensing Inc. | Face identification using millimeter-wave radar sensor data |
| JPWO2024161784A1 (es) | 2023-01-30 | 2024-08-08 | ||
| WO2024161783A1 (ja) | 2023-01-30 | 2024-08-08 | 富士フイルム株式会社 | 学習支援装置、学習支援装置の作動方法、および学習支援装置の作動プログラム |
| WO2024242686A1 (en) * | 2023-05-24 | 2024-11-28 | Wyatt Technology, Llc | Detecting bubbles in images of a sample in a well plate |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2104699T3 (es) | 1990-03-30 | 1997-10-16 | Neuromedical Systems Inc | Metodo y sistema automatizados de clasificar una muestra citologica. |
| DE19616997A1 (de) | 1996-04-27 | 1997-10-30 | Boehringer Mannheim Gmbh | Verfahren zur automatisierten mikroskopunterstützten Untersuchung von Gewebeproben oder Körperflüssigkeitsproben |
| US6753161B2 (en) | 1997-03-27 | 2004-06-22 | Oncosis Llc | Optoinjection methods |
| EP2463653A1 (en) | 2009-08-07 | 2012-06-13 | Nikon Corporation | Technique for classifying cells, image processing program and image processing device using the technique, and method for producing cell mass |
| EP2739937B1 (en) | 2011-08-02 | 2023-03-01 | The Regents of The University of California | Rapid, massively parallel single-cell drug response measurements via live cell interferometry |
| EP2682187B1 (en) * | 2012-07-02 | 2017-09-13 | Molecular Devices, LLC | Selection method and apparatus |
| EP2708890B1 (en) | 2012-09-14 | 2015-11-18 | Molecular Devices (New Milton) Ltd | Method of selecting a monoclonal cell colony |
| US9619881B2 (en) * | 2013-09-26 | 2017-04-11 | Cellogy, Inc. | Method and system for characterizing cell populations |
| JP5850205B2 (ja) * | 2013-11-27 | 2016-02-03 | 大日本印刷株式会社 | 培地情報登録システム、コロニー検出装置、プログラム及び衛生管理システム |
| ES2772300T3 (es) * | 2015-06-02 | 2020-07-07 | Baxalta Inc | Método para recoger una colonia de células |
| CN110914666A (zh) | 2017-05-19 | 2020-03-24 | 兴盛生物科技股份有限公司 | 用于计数细胞的系统和方法 |
| CN108596046A (zh) | 2018-04-02 | 2018-09-28 | 上海交通大学 | 一种基于深度学习的细胞检测计数方法及系统 |
| GB2576191B (en) * | 2018-08-08 | 2022-11-16 | Sphere Fluidics Ltd | Droplet processing methods and systems |
-
2019
- 2019-11-26 BR BR112021010404-6A patent/BR112021010404A2/pt unknown
- 2019-11-26 CA CA3120281A patent/CA3120281A1/en active Pending
- 2019-11-26 AU AU2019387079A patent/AU2019387079B2/en active Active
- 2019-11-26 SG SG11202105399RA patent/SG11202105399RA/en unknown
- 2019-11-26 MX MX2021005938A patent/MX2021005938A/es unknown
- 2019-11-26 EA EA202191499A patent/EA202191499A1/ru unknown
- 2019-11-26 JP JP2021529789A patent/JP7789560B2/ja active Active
- 2019-11-26 EP EP19824078.0A patent/EP3888004B1/en active Active
- 2019-11-26 WO PCT/US2019/063177 patent/WO2020112723A1/en not_active Ceased
- 2019-11-26 US US17/296,570 patent/US12561992B2/en active Active
- 2019-11-26 KR KR1020217019305A patent/KR20210099028A/ko active Pending
- 2019-11-26 IL IL283577A patent/IL283577B2/en unknown
- 2019-11-26 CN CN201980077951.XA patent/CN113168519A/zh active Pending
-
2021
- 2021-05-28 CL CL2021001409A patent/CL2021001409A1/es unknown
-
2024
- 2024-10-02 JP JP2024173003A patent/JP2025013804A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| AU2019387079B2 (en) | 2024-08-01 |
| JP2022509201A (ja) | 2022-01-20 |
| SG11202105399RA (en) | 2021-06-29 |
| IL283577A (en) | 2021-07-29 |
| MX2021005938A (es) | 2021-06-30 |
| IL283577B1 (en) | 2025-12-01 |
| BR112021010404A2 (pt) | 2021-08-24 |
| JP7789560B2 (ja) | 2025-12-22 |
| CN113168519A (zh) | 2021-07-23 |
| EP3888004A1 (en) | 2021-10-06 |
| AU2019387079A1 (en) | 2021-06-03 |
| WO2020112723A1 (en) | 2020-06-04 |
| CA3120281A1 (en) | 2020-06-04 |
| EP3888004B1 (en) | 2026-03-18 |
| EA202191499A1 (ru) | 2021-08-20 |
| US12561992B2 (en) | 2026-02-24 |
| IL283577B2 (en) | 2026-04-01 |
| US20220012465A1 (en) | 2022-01-13 |
| KR20210099028A (ko) | 2021-08-11 |
| JP2025013804A (ja) | 2025-01-28 |
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