CN101124316A - 用于转染真核细胞的固定的可降解阳离子聚合物 - Google Patents

用于转染真核细胞的固定的可降解阳离子聚合物 Download PDF

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Publication number
CN101124316A
CN101124316A CNA2005800434531A CN200580043453A CN101124316A CN 101124316 A CN101124316 A CN 101124316A CN A2005800434531 A CNA2005800434531 A CN A2005800434531A CN 200580043453 A CN200580043453 A CN 200580043453A CN 101124316 A CN101124316 A CN 101124316A
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transfection
cell
formula
degradable
cells
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Chinese (zh)
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田中康进
克里斯·P·卡斯特勒
俞磊
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Nitto Denko Corp
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Nitto Denko Corp
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/30Synthetic polymers

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CNA2005800434531A 2004-12-17 2005-12-14 用于转染真核细胞的固定的可降解阳离子聚合物 Pending CN101124316A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63734404P 2004-12-17 2004-12-17
US60/637,344 2004-12-17

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CN101124316A true CN101124316A (zh) 2008-02-13

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Country Status (7)

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EP (1) EP1836293A2 (fr)
JP (1) JP4739352B2 (fr)
KR (2) KR20130010036A (fr)
CN (1) CN101124316A (fr)
AU (1) AU2005316501B2 (fr)
CA (1) CA2590820A1 (fr)
WO (1) WO2006066001A2 (fr)

Cited By (5)

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CN102827756A (zh) * 2012-06-25 2012-12-19 华南农业大学 应用于大豆下胚轴外植体转化受体系统的农杆菌局部侵染方法和装置
CN105579584A (zh) * 2013-06-28 2016-05-11 埃泽瑞斯公司 用于将rna引入细胞的组合物
CN109735573A (zh) * 2019-02-19 2019-05-10 武汉普诺赛生命科技有限公司 一种瞬时转染试剂及其应用方法
WO2022068884A1 (fr) * 2020-09-30 2022-04-07 上海交通大学 Procédé et système d'administration d'acide nucléique
CN114630855A (zh) * 2019-09-25 2022-06-14 爱尔兰国立都柏林大学 超支化阳离子聚合物作为转染细胞的核酸递送载体

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US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP3718635A1 (fr) 2003-07-31 2020-10-07 Handylab, Inc. Traitement d'échantillons contenant des particules
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US8603824B2 (en) 2004-07-26 2013-12-10 Pfenex, Inc. Process for improved protein expression by strain engineering
US7358223B2 (en) 2004-10-04 2008-04-15 Nitto Denko Corporation Biodegradable cationic polymers
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
ES2692380T3 (es) 2006-03-24 2018-12-03 Handylab, Inc. Método para realizar PCR con un cartucho con varias pistas
US8765076B2 (en) 2006-11-14 2014-07-01 Handylab, Inc. Microfluidic valve and method of making same
WO2008060604A2 (fr) 2006-11-14 2008-05-22 Handylab, Inc. Système microfluidique utilisé pour amplifier et détecter des polynucléotides en parallèle
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
JP5444553B2 (ja) * 2007-04-27 2014-03-19 フェネックス インコーポレイテッド 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および/または質が改善されている特定の株を同定する方法
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
AU2008276211B2 (en) * 2007-07-13 2015-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
JP6088487B2 (ja) 2011-04-15 2017-03-01 ベクトン・ディキンソン・アンド・カンパニーBecton, Dickinson And Company 走査リアルタイムマイクロ流体熱サイクラーと同期熱サイクリング及び走査光学検出の方法
RU2622432C2 (ru) 2011-09-30 2017-06-15 Бектон, Дикинсон Энд Компани Унифицированная полоска для реактивов
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
WO2013067202A1 (fr) 2011-11-04 2013-05-10 Handylab, Inc. Dispositif de préparation d'échantillons de polynucléotides
AU2013214849B2 (en) 2012-02-03 2016-09-01 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
EP3218012A1 (fr) * 2014-11-10 2017-09-20 ethris GmbH Induction de l'ostéogenèse par administration d'arn codant pour bmp
HUE052022T2 (hu) * 2016-10-05 2021-04-28 Syngenta Participations Ag Géncsendesítésben történõ vagy géncsendesítéshez kapcsolódó javítások
CN114904003B (zh) * 2021-02-09 2023-09-29 广州立得生物医药科技有限公司 可电离的阳离子脂质类似物材料在作为核酸药物递送载体或转染试剂中的应用

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JP2003509060A (ja) * 1999-09-17 2003-03-11 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ 逆トランスフェクション方法
US20020006664A1 (en) * 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
ATE351902T1 (de) * 2000-11-03 2007-02-15 Univ Michigan Oberflächentransfektion und expressionsverfahren
US20030215395A1 (en) * 2002-05-14 2003-11-20 Lei Yu Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier
JP2005529959A (ja) * 2002-06-14 2005-10-06 マイラス コーポレイション 細胞へのポリヌクレオチドの伝達をするための新規な方法
US20040048260A1 (en) * 2002-09-10 2004-03-11 Fu-Hsiung Chang Transfection of nucleic acid
US20040138154A1 (en) * 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
US6878374B2 (en) * 2003-02-25 2005-04-12 Nitto Denko Corporation Biodegradable polyacetals
GB0320627D0 (en) * 2003-09-03 2003-10-01 Imp College Innovations Ltd Methods
US7125709B2 (en) * 2004-02-10 2006-10-24 Nitto Denko Corporation Culture device and method for eukaryotic cell transfection
EP1723240B1 (fr) * 2004-02-18 2010-08-11 Nitto Denko Corporation Dispositif de culture et procede de transfection de cellule eucaryote

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102827756A (zh) * 2012-06-25 2012-12-19 华南农业大学 应用于大豆下胚轴外植体转化受体系统的农杆菌局部侵染方法和装置
CN102827756B (zh) * 2012-06-25 2014-04-09 华南农业大学 应用于大豆下胚轴外植体转化受体系统的农杆菌局部侵染方法和装置
CN105579584A (zh) * 2013-06-28 2016-05-11 埃泽瑞斯公司 用于将rna引入细胞的组合物
CN105579584B (zh) * 2013-06-28 2020-08-28 埃泽瑞斯公司 用于将rna引入细胞的组合物
CN109735573A (zh) * 2019-02-19 2019-05-10 武汉普诺赛生命科技有限公司 一种瞬时转染试剂及其应用方法
CN114630855A (zh) * 2019-09-25 2022-06-14 爱尔兰国立都柏林大学 超支化阳离子聚合物作为转染细胞的核酸递送载体
WO2022068884A1 (fr) * 2020-09-30 2022-04-07 上海交通大学 Procédé et système d'administration d'acide nucléique

Also Published As

Publication number Publication date
CA2590820A1 (fr) 2006-06-22
JP4739352B2 (ja) 2011-08-03
EP1836293A2 (fr) 2007-09-26
AU2005316501B2 (en) 2010-10-21
AU2005316501A1 (en) 2006-06-22
WO2006066001A3 (fr) 2006-09-08
KR20130010036A (ko) 2013-01-24
KR20070101859A (ko) 2007-10-17
WO2006066001A2 (fr) 2006-06-22
KR101263173B1 (ko) 2013-05-13
JP2008523810A (ja) 2008-07-10

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