CN101628126A - Natural polypeptides material for biomineralization and preparation method thereof - Google Patents
Natural polypeptides material for biomineralization and preparation method thereof Download PDFInfo
- Publication number
- CN101628126A CN101628126A CN200910060369A CN200910060369A CN101628126A CN 101628126 A CN101628126 A CN 101628126A CN 200910060369 A CN200910060369 A CN 200910060369A CN 200910060369 A CN200910060369 A CN 200910060369A CN 101628126 A CN101628126 A CN 101628126A
- Authority
- CN
- China
- Prior art keywords
- silk
- spider
- polypeptide
- natural
- mineralization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 64
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 63
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 63
- 239000000463 material Substances 0.000 title claims abstract description 51
- 230000033558 biomineral tissue development Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 108010022355 Fibroins Proteins 0.000 claims abstract description 41
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 28
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 28
- 229920001872 Spider silk Polymers 0.000 claims abstract description 22
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000011787 zinc oxide Substances 0.000 claims abstract description 9
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 6
- 239000013078 crystal Substances 0.000 claims abstract description 5
- 241000238631 Hexapoda Species 0.000 claims abstract description 4
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims abstract description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 3
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 3
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 3
- 235000011010 calcium phosphates Nutrition 0.000 claims abstract description 3
- 229910000428 cobalt oxide Inorganic materials 0.000 claims abstract description 3
- IVMYJDGYRUAWML-UHFFFAOYSA-N cobalt(ii) oxide Chemical compound [Co]=O IVMYJDGYRUAWML-UHFFFAOYSA-N 0.000 claims abstract description 3
- AJNVQOSZGJRYEI-UHFFFAOYSA-N digallium;oxygen(2-) Chemical compound [O-2].[O-2].[O-2].[Ga+3].[Ga+3] AJNVQOSZGJRYEI-UHFFFAOYSA-N 0.000 claims abstract description 3
- 229910001195 gallium oxide Inorganic materials 0.000 claims abstract description 3
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 claims abstract description 3
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims abstract description 3
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 claims abstract 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 18
- 241000255789 Bombyx mori Species 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000006460 hydrolysis reaction Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 108010013296 Sericins Proteins 0.000 claims description 7
- 238000000502 dialysis Methods 0.000 claims description 7
- 230000007062 hydrolysis Effects 0.000 claims description 7
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 230000020477 pH reduction Effects 0.000 claims description 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
- DSSYKIVIOFKYAU-XCBNKYQSSA-N (R)-camphor Chemical compound C1C[C@@]2(C)C(=O)C[C@@H]1C2(C)C DSSYKIVIOFKYAU-XCBNKYQSSA-N 0.000 claims description 2
- 241000723346 Cinnamomum camphora Species 0.000 claims description 2
- 229960000846 camphor Drugs 0.000 claims description 2
- 229930008380 camphor Natural products 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 241000207965 Acanthaceae Species 0.000 claims 1
- 241000282376 Panthera tigris Species 0.000 claims 1
- 241000309023 Pirata subpiraticus Species 0.000 claims 1
- 235000004443 Ricinus communis Nutrition 0.000 claims 1
- 241000356457 Salticidae Species 0.000 claims 1
- 238000005119 centrifugation Methods 0.000 claims 1
- CGMRCMMOCQYHAD-UHFFFAOYSA-J dicalcium hydroxide phosphate Chemical compound [OH-].[Ca++].[Ca++].[O-]P([O-])([O-])=O CGMRCMMOCQYHAD-UHFFFAOYSA-J 0.000 claims 1
- 235000017550 sodium carbonate Nutrition 0.000 claims 1
- 239000002131 composite material Substances 0.000 abstract description 3
- 235000019738 Limestone Nutrition 0.000 abstract 1
- DMGNFLJBACZMRM-UHFFFAOYSA-N O[P] Chemical compound O[P] DMGNFLJBACZMRM-UHFFFAOYSA-N 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 235000010216 calcium carbonate Nutrition 0.000 abstract 1
- 239000006028 limestone Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 25
- 239000002917 insecticide Substances 0.000 description 8
- 241000239290 Araneae Species 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000002245 particle Substances 0.000 description 4
- 239000003708 ampul Substances 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 241000255794 Bombyx mandarina Species 0.000 description 2
- 241000239292 Theraphosidae Species 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000001143 conditioned effect Effects 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 230000000887 hydrating effect Effects 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 235000003846 Ricinus Nutrition 0.000 description 1
- 241000322381 Ricinus <louse> Species 0.000 description 1
- 241000256007 Samia cynthia Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000003519 biomedical and dental material Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000013066 combination product Substances 0.000 description 1
- 229940127555 combination product Drugs 0.000 description 1
- 239000011246 composite particle Substances 0.000 description 1
- 239000002178 crystalline material Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000001089 mineralizing effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明涉及一种生物矿化用天然多肽材料及其制备方法，特别是涉及一种可用于矿化制备具有良好生物相容性晶体结构的天然多肽材料，属于生物医用材料领域。含有天然丝蛋白的多肽材料是来自昆虫丝的脱胶丝蛋白和蜘蛛丝蛋白，这种多肽材料能够用于室温矿化的晶体包括氧化锌，氧化铁、氧化镓、氧化钴、氧化钛、羟基磷灰石、磷酸钙、碳酸钙。脱胶后的丝素蛋白溶液在水洗以及加酸水解后；再经过透析、离心浓缩后获得矿化用天然多肽材料。它能够用于矿化制备具有良好生物相容性的复合材料。The invention relates to a natural polypeptide material for biomineralization and a preparation method thereof, in particular to a natural polypeptide material with a good biocompatible crystal structure that can be used for mineralization and belongs to the field of biomedical materials. Polypeptide materials containing natural silk proteins are degummed silk proteins and spider silk proteins from insect silk. The crystals that can be used for mineralization at room temperature include zinc oxide, iron oxide, gallium oxide, cobalt oxide, titanium oxide, and hydroxyl phosphorus. Limestone, calcium phosphate, calcium carbonate. After the degummed silk fibroin solution is washed with water and hydrolyzed with acid, it is then dialyzed and centrifugally concentrated to obtain natural polypeptide materials for mineralization. It can be used for mineralization to prepare composite materials with good biocompatibility.
Description
Technical field
The present invention relates to a kind of natural polypeptides material for biomineralization and preparation method thereof, particularly relate to a kind of natural polypeptides material that the mineralising preparation has the good biocompatibility crystal structure that can be used for, belong to biomedical materials field.
Background technology
Most of Araneas and lepidopteran insects such as silkworm, Antherea pernyi Guerin-Meneville etc. can both produce a large amount of fibroins.These fibroins have also obtained development and utilization except being used for the textile raw material in many fields such as medicine, daily-use chemical industry, Aero-Space.Along with molecular biological fast development, people can obtain the natural silk albumen of high-performance, high yield by the method for gene modification in recent years.These exploitations of studying successfully to realizing fiber spinning from crystalline state efficiently and new bio medical material from now on lay the foundation.At present, obtained preliminary progress for biomineralization with the research of polypeptide, have been found that some artificial synthetic short chain polypeptides and collagen can become utility model (the Jeffrey D.Hartgerink etc. of synthetic multilevel hierarchy mineralizing material, Science 2001,294, p1684), these researchs relate to molecular biology and multi-field scientific knowledge such as material science and medical science.
Fibroin mainly is made of fibroin and sericin.Sericin is because of relatively poor biocompatibility and biological sensitization, and must get rid of.The normal method that adopts low concentration alkali solution (as sodium carbonate liquor, sodium hydroxide solution etc.) to carry out decocting in water is separated removal with it with fibroin albumen.Because fibroin protein has good biocompatibility, biodegradability and workability simultaneously, make it be widely used in the biomedical materials field, as carrier, artificial bone tissue and the artificial joint etc. of drug release carrier, artificial skin, immobilized enzyme, some of them achievement in research (as artificial skin) has entered the clinical practice stage.Used in the present invention fibroin is insecticide fibroin albumen or the spider silk fibroin that obtains behind the sericin removal.
Tradition thinks, ZnO particle has nontoxic and biological safety and be used to daily cosmetics, pharmaceutical carrier and medical packing material (M.Ito, Biomaterials, 1991,12, p41; And D.Orstavik, J.K.Hongslo, Biomaterials, 1985,6, p129).But existing nanometer or the micron ZnO particles of studies show that has toxicity to many tissues or organ.These toxicity are because (Y.Osamu, InternationalJournal of Inorganic Materials, 2001,3, the p643 that the bio-incompatibility composition of ZnO particle surface causes; And P.K.Stoimenov, R.L.Klinger, G.L.Marchin, K.J.Klabunde, Langmuir, 2002,18, p6679), thereby limited the ZnO particle material in the clinical and application daily cosmetics field.Recently, people adopt artificial polypeptide room temperature mineralising and have prepared the nano-ZnO composite particles.But owing to this artificial polypeptide complicated process of preparation, cost height, and artificial polypeptide chain is relatively lacked and be difficult to make the ZnO granule to have excellent biological compatibility.
The polypeptide product that the acidified degradation of the present invention by good insecticide fibroin albumen of biocompatibility and spider silk fibroin obtains is as the template of biomineralization under the room temperature, to obtain the good polypeptide combination product of biocompatibility.
Summary of the invention
The objective of the invention is to propose a kind of crystalline polypeptide material of biomineralization and preparation method thereof that is used to prepare, substitute the polypeptide material of expensive synthetic with natural silk polypeptide material with good biocompatibility, to reduce the crystalline preparation cost of biomineralization, be beneficial to of the application of these biomineralization crystalline materials at biomedical engineering field.
The present invention proposes is used to prepare the crystalline polypeptide material of biomineralization, and it is characterized in that: contain the proteic peptide molecule of natural silk in this material, described natural silk albumen is degumed silk albumen or does not have collodion silk albumen.
Use in the natural silk protein polypeptide material in above-mentioned mineralising, the fibroin in the described material is from insecticide silk, spider silk.
Use in the natural silk protein polypeptide material in above-mentioned mineralising, described insecticide silk is domestic silkworm silk, tussah, tussah silk, wild silk yarn, ricinus silk, camphor silk, fogara silk, bag moth silk, moth silk.
Above-mentioned mineralising with natural silk protein polypeptide material in, described spider silk for the circle spider's thread, the golden spider's thread, tarantula silk, the brave spider's thread, jump the spider's thread, the former spider's thread, in the prominent spider's thread, the long foot spider's thread.
Use in the natural silk protein polypeptide material in above-mentioned mineralising, described fibroin is the water-solubility protein polypeptide.
Use in the natural silk protein polypeptide material in above-mentioned mineralising, described polypeptide material can be used in room temperature mineralising crystal, comprises zinc oxide, ferrum oxide, gallium oxide, cobalt oxide, titanium oxide, hydroxyapatite, calcium phosphate, calcium carbonate.
The present invention proposes the method for a kind of mineralising with the fibroin polypeptide, and it is characterized in that: described method comprises the following steps: successively
(1) fibroin is put into the Na of 15%-15%
2CO
3In the solution, in beaker, boiled 10-30 minute, in solution, have colloidal sol shape material sericin to generate; When adopting spider silk fibroin to prepare polypeptide material, this step can inoperation.
(2) fibroin after coming unstuck in the step (1) or the spider silk that do not pass through step (1) are cleaned with deionized water, adding concentration is the hydrochloric acid of 1～10 mol, and the acidification hydrolization reaction is 5～36 hours under 80 ℃～130 ℃ temperature;
(3) pH value of hydrating solution is 7 in the regulating step (2), the polypeptide solution after this hydrolysis is placed in the bag filter again, places in 4 ℃ of refrigerators, changes water every day once to three times;
(4) clear solution that dialysis back in the step (3) is obtained promptly is the fibroin polypeptide material, places standby in 4 ℃ of refrigerators through centrifugal concentrated back.
The present invention proposes the method for a kind of mineralising with the fibroin polypeptide, and it is characterized in that: the hydrochloric acid in the described step (2) can be changed into the NaOH solution with 0.1～1 mol, 80 ℃ of following hydrolysis 1～20 hour.
The prepared fibroin polypeptide material of the present invention can be used in the preparation of biomineralization composite, and makes the composite of preparation have excellent biological compatibility and biological activity.
The specific embodiment
The present invention will be further described below in conjunction with 3 embodiment, but embodiment of the present invention are not limited thereto.
Fibroin used among the present invention can be insecticide silk or spider silk, the insecticide silk can be silkworm, Bombyx mandarina Moore, Antherea pernyi Guerin-Meneville, giant silkworm, Semen Ricini silkworm, wild silkworm, Philosamia cynthia, bag moth, moth insecticide silk etc., sericin in the insecticide silk to be removed preferred domestic silkworm silk in the invention before using.The silk fibroin protein that will come unstuck is dissolved in the hydrochloric acid that concentration is 1～10 mol, 80～120 ℃ of following acidification hydrolizations of temperature 5～36 hours, then with the protein polypeptide of hydrolysis through desalination, concentration.The mineralising of the present invention's preparation i.e. protein polypeptide material after acidification hydrolization is handled of fibroin polypeptide.
The mineralising that the present invention proposes mainly comprises the following steps: with the preparation method of fibroin polypeptide material
(1) fibroin is put into the Na of 10%-25%
2CO
3In the solution, in beaker, boiled 10～30 minutes, in solution, have colloidal sol shape material sericin to generate; When adopting spider silk fibroin to prepare polypeptide material, this step can inoperation.
(2) fibroin after coming unstuck in the step (1) or the spider silk that do not pass through step (1) are cleaned with deionized water, adding concentration is the hydrochloric acid of 1 mol～10 mol, and the acidification hydrolization reaction is 5～24 hours under 80 ℃ of temperature;
(3) pH value of hydrating solution is 7 in the regulating step (2), the polypeptide solution after this hydrolysis is placed in the bag filter again, places in 4 ℃ of refrigerators, changes water every day once～three time;
(4) promptly be mineralising fibroin polypeptide material obtaining clear solution after the dialysis in the step (3), standby through putting into 4 ℃ of refrigerators after centrifugal the concentrating.
Embodiment 1
In the concentration that modulates is that the 50ml of 2wt% comes unstuck in the domestic silkworm silk protein solution, after Dropwise 5 0ml concentration is the hydrochloric acid solution of 7 mol, pours in the ampoule bottle, with nitrogen protection and with the bottle sealing, puts into 80 ℃ of water insulations 8 hours behind the evacuation.Fibroin albumen polypeptide solution after the hydrolysis is conditioned pH value to 7, inserts bag filter, dialyses one day in deionized water.Fibroin albumen polypeptide solution after the dialysis was removed small amount of precipitate in centrifugal 15 minutes at 5000rpm, got supernatant and was mineralising fibroin polypeptide material, and it is standby to place 4 ℃ of refrigerators to preserve.The concentration of prepared fibroin albumen polypeptide solution is 0.1～0.2mg/mL.
Embodiment 2
In the concentration that modulates is that the 60ml of 3wt% comes unstuck in the domestic silkworm silk protein solution, drips after 60ml concentration is the hydrochloric acid solution of 5 mol, pours in the ampoule bottle, with nitrogen protection and with the bottle sealing, puts into 90 ℃ of water insulations 6 hours behind the evacuation.Fibroin albumen polypeptide solution after the hydrolysis is conditioned pH value to 7, inserts bag filter, dialyses two days in deionized water.Fibroin albumen polypeptide solution after the dialysis was removed small amount of precipitate in centrifugal 15 minutes at 5000rpm, got supernatant and was mineralising fibroin polypeptide material, and it is standby to place 4 ℃ of refrigerators to preserve.The concentration of prepared fibroin albumen polypeptide solution is 0.2～0.4mg/mL.
Embodiment 3
1) is in the 60ml tarantula fibroin liquid of 3wt% in the concentration that modulates, after dropping 60ml concentration is the hydrochloric acid solution of 6 mol, pours in the ampoule bottle, with nitrogen protection and with the bottle sealing, put into 90 ℃ of water insulations 6 hours behind the evacuation.
2) above-mentioned system solution is regulated pH value to 7, insert bag filter, in deionized water two days.Solution after the dialysis is got supernatant and is mineralising fibroin polypeptide material through removing small amount of precipitate, and it is standby to place 4 ℃ of refrigerators to preserve.The concentration of prepared fibroin albumen polypeptide solution is 0.2～0.4mg/mL.
Claims (8)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910060369A CN101628126A (en) | 2009-08-18 | 2009-08-18 | Natural polypeptides material for biomineralization and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910060369A CN101628126A (en) | 2009-08-18 | 2009-08-18 | Natural polypeptides material for biomineralization and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101628126A true CN101628126A (en) | 2010-01-20 |
Family
ID=41573514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910060369A Pending CN101628126A (en) | 2009-08-18 | 2009-08-18 | Natural polypeptides material for biomineralization and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101628126A (en) |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103628119A (en) * | 2013-12-03 | 2014-03-12 | 苏州大学 | Preparation method for bionic porous octacalcium phosphate/silk fibroin composite film layer |
| CN103897021A (en) * | 2013-02-04 | 2014-07-02 | 中国科学院上海有机化学研究所 | Silk peptide as well as preparation method and application thereof |
| CN104250926A (en) * | 2014-09-25 | 2014-12-31 | 苏州印丝特纺织数码科技有限公司 | Production method of hydroxyapatite-functionalized silk fabric |
| CN106620861A (en) * | 2016-12-09 | 2017-05-10 | 宁波芸生纺织品科技有限公司 | Silk fibroin/strontium carbonate composite material and preparation method thereof |
| CN106754665A (en) * | 2016-12-28 | 2017-05-31 | 浙江大学 | The micron-nano topological structure of Derived from Mesenchymal Stem Cells is promoted to prepare and differentiation method |
| CN107050528A (en) * | 2017-05-05 | 2017-08-18 | 重庆工程职业技术学院 | The preparation method of Archaeological iron with Bone Defect Repari |
| CN107595661A (en) * | 2017-11-08 | 2018-01-19 | 海南蛛王药业有限公司 | A kind of cosmetics comprising spider silk hydrolysate and preparation method thereof |
| CN107652354A (en) * | 2017-11-08 | 2018-02-02 | 海南蛛王药业有限公司 | A kind of spider silk hydrolysate and preparation method thereof |
| CN108244657A (en) * | 2018-01-12 | 2018-07-06 | 海南蛛王药业有限公司 | A kind of method for hydrolysis of spider silk |
| CN108498861A (en) * | 2018-06-28 | 2018-09-07 | 河南省肿瘤医院 | A kind of preparation method of hydroxyapatite-fibroin-titanium dioxide biomaterial |
| CN110028569A (en) * | 2019-05-06 | 2019-07-19 | 东华大学 | A kind of preparation method of natural spider silk albumen powder |
| CN110305202A (en) * | 2019-07-03 | 2019-10-08 | 浙江大学 | A method of improving fibroin albumen biomineralisation capabilities |
| CN113661283A (en) * | 2019-03-13 | 2021-11-16 | 国立研究开发法人农业·食品产业技术综合研究机构 | Fiber-reinforced composite material and method for producing same |
| CN114209845A (en) * | 2021-12-18 | 2022-03-22 | 苏州健雄职业技术学院 | A kind of preparation method of magnetic nanoparticles |
-
2009
- 2009-08-18 CN CN200910060369A patent/CN101628126A/en active Pending
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897021A (en) * | 2013-02-04 | 2014-07-02 | 中国科学院上海有机化学研究所 | Silk peptide as well as preparation method and application thereof |
| CN103897021B (en) * | 2013-02-04 | 2016-06-29 | 中国科学院上海有机化学研究所 | Silk peptide, preparation method and application |
| CN103628119A (en) * | 2013-12-03 | 2014-03-12 | 苏州大学 | Preparation method for bionic porous octacalcium phosphate/silk fibroin composite film layer |
| CN104250926A (en) * | 2014-09-25 | 2014-12-31 | 苏州印丝特纺织数码科技有限公司 | Production method of hydroxyapatite-functionalized silk fabric |
| CN106620861A (en) * | 2016-12-09 | 2017-05-10 | 宁波芸生纺织品科技有限公司 | Silk fibroin/strontium carbonate composite material and preparation method thereof |
| CN106754665A (en) * | 2016-12-28 | 2017-05-31 | 浙江大学 | The micron-nano topological structure of Derived from Mesenchymal Stem Cells is promoted to prepare and differentiation method |
| CN107050528B (en) * | 2017-05-05 | 2018-02-16 | 重庆工程职业技术学院 | The preparation method of Archaeological iron with Bone Defect Repari |
| CN107050528A (en) * | 2017-05-05 | 2017-08-18 | 重庆工程职业技术学院 | The preparation method of Archaeological iron with Bone Defect Repari |
| CN107652354B (en) * | 2017-11-08 | 2021-02-02 | 海南蛛王生物科技有限公司 | Spider silk hydrolysate and preparation method thereof |
| CN107652354A (en) * | 2017-11-08 | 2018-02-02 | 海南蛛王药业有限公司 | A kind of spider silk hydrolysate and preparation method thereof |
| CN107595661B (en) * | 2017-11-08 | 2020-09-15 | 海南蛛王生物科技有限公司 | A cosmetic containing spider silk hydrolysate and its preparation method |
| CN107595661A (en) * | 2017-11-08 | 2018-01-19 | 海南蛛王药业有限公司 | A kind of cosmetics comprising spider silk hydrolysate and preparation method thereof |
| CN108244657A (en) * | 2018-01-12 | 2018-07-06 | 海南蛛王药业有限公司 | A kind of method for hydrolysis of spider silk |
| CN108498861A (en) * | 2018-06-28 | 2018-09-07 | 河南省肿瘤医院 | A kind of preparation method of hydroxyapatite-fibroin-titanium dioxide biomaterial |
| CN113661283A (en) * | 2019-03-13 | 2021-11-16 | 国立研究开发法人农业·食品产业技术综合研究机构 | Fiber-reinforced composite material and method for producing same |
| CN110028569A (en) * | 2019-05-06 | 2019-07-19 | 东华大学 | A kind of preparation method of natural spider silk albumen powder |
| CN110305202A (en) * | 2019-07-03 | 2019-10-08 | 浙江大学 | A method of improving fibroin albumen biomineralisation capabilities |
| CN110305202B (en) * | 2019-07-03 | 2021-06-08 | 浙江大学 | Method for improving biomineralization capability of silk fibroin |
| CN114209845A (en) * | 2021-12-18 | 2022-03-22 | 苏州健雄职业技术学院 | A kind of preparation method of magnetic nanoparticles |
| CN114209845B (en) * | 2021-12-18 | 2023-11-07 | 苏州健雄职业技术学院 | A kind of preparation method of magnetic nanoparticles |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101628126A (en) | Natural polypeptides material for biomineralization and preparation method thereof | |
| WO2002034885A1 (en) | Sericin-containing material, process for producing the same and method of using the same | |
| CN102732586B (en) | Preparation method of mesenchymal stem cell secretin | |
| CN101422205A (en) | Preparation method of sea cucumber intestine polypeptide capsule | |
| CN102294049B (en) | Bioactive glass and chitosan composite bone repair material and preparation method and application thereof | |
| CN101736430A (en) | Method for preparing silk fibroin nano-fibre with skin-care effect | |
| CN101244277A (en) | Silk fibroin drug-loaded microspheres and preparation method thereof | |
| CN101250574A (en) | A kind of preparation method of step molecular weight cod skin collagen peptide | |
| CN101947335B (en) | Bacteria cellulose/gelatin/hydroxyapatite composite material and preparation method thereof | |
| CN104984393B (en) | A kind of bone tissue engineering stent material and preparation method thereof | |
| CN101327341A (en) | A preparation method of nano-scale silk protein-hydroxyapatite mineralized composite fiber | |
| CN103386144A (en) | Preparation method of fish collagen combined chitosan biological dressing | |
| CN103554520A (en) | Ion-induced method for preparing tussah silk fibroin nanoparticles | |
| CN117736305A (en) | A new type I recombinant collagen, its production method and use | |
| CN101829025A (en) | Bioactive facial mask and preparation method therefore | |
| CN107540744A (en) | Recombined collagen and its temperature-sensitive hydrogel | |
| CN109601445A (en) | A kind of cultivation method for improving pearl production of Pinctada martensii particles | |
| CN1241654C (en) | Mineralized fibroin material and preparation method | |
| CN101580978A (en) | Marine biological antibacterial nano fiber and preparation method thereof | |
| CN101245342B (en) | Technique for producing gene rHu(Cu/Zn-SOD) with one-step method | |
| US10576159B2 (en) | Method for preparing an induced osteogenesis formulation | |
| CN102277405A (en) | Preparation method of snakeskin active peptide | |
| CN106943629B (en) | Nano artificial bone carrying Thai-energy hydroxyapatite-enzymolysis ossein protein | |
| CN107149701B (en) | I type collagen/hydroxyapatite calcium bionic bone loaded with carbapenem antibiotics | |
| CN105617463A (en) | Preparation method of polyactic acid composite funnel spider silk protein modified calcium polyphosphate bio-bone scaffold |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20100120 |