CN101731237A - Acibenzolar-containing sterilizing composition - Google Patents

Abstract

The invention relates to a bactericidal composition containing activated ester, the active ingredients of which are activated ester (A) and a pyridine bactericide (B), wherein the pyridine bactericide is selected from the group consisting of fluazinam, pyridoxine, and boscalid The mass ratio of A to B is 100:1 to 1:100, and the mass percentage of active components in the composition is 10 to 80%. The composition can be formulated into formulations such as emulsifiable concentrate, suspension concentrate, water emulsion, wettable powder, water dispersible granule, microemulsion and the like. The application places of the fungicidal composition of the present invention are farmlands, orchards, etc., and are used to prevent and control various plant diseases, especially powdery mildew, downy mildew, and blight on crops such as grains, vegetables, fruit trees, and flowers.

Description

Fungicidal compositions containing activated esters

technical field

The invention relates to a bactericidal composition, in particular to a bactericidal composition containing an activated ester and a pyridine bactericide, and belongs to the field of agricultural bactericides.

Background technique

Powdery mildew is a plant disease caused by fungi of the family Erysiphacesae. Powdery mildew is widespread all over the world, especially in dicotyledonous plants. The main powdery mildews in China include cereal powdery mildew caused by Ery-siphe graminis; powdery mildew of melons, beans, hemp and other plants caused by Sphaerotheca fuliginea; Grape powdery mildew caused by Uncinula necator; powdery mildew of more than 80 kinds of trees such as mulberry, pear, persimmon, walnut, chestnut, litchi and papaya caused by Phyllactinia corylea; Powdery mildew of Rosa flowers caused by Sphaerotheca pannosa.

When the powdery mildew first occurs, the leaves produce small yellow spots, and then expand and develop into round or oval lesions, with a white powdery mold layer on the surface, which are the conidiophores and conidia of the fungus, and the lesions later turn yellowish brown Closing shell for bacteria. The mildew spots are scattered separately in the early stage, and then combined to form a large mildew spot, which can even cover the whole leaf, seriously affecting photosynthesis, disturbing normal metabolism, causing premature aging, and loss of yield.

Downy mildew is one of the most important diseases in vegetable production. Downy mildew can occur at all stages from seedlings to harvest, and the adult plants suffer the most. It mainly damages the leaves and develops from the base to the upper leaves. In the early stage of the disease, light yellow sub-circular to polygonal lesions are formed on the leaves. When the air is humid, a frosty mold layer is formed on the back of the leaves, and sometimes it can spread to the leaves. In the later stage, the disease spots are withered and contiguous, and they are yellowish brown. In severe cases, all the outer leaves are withered and yellow and die. Field planting is too dense, watering too early after planting, too large, high soil moisture, poor drainage, etc. are prone to disease. Continuous rainy weather in late spring, early summer or autumn is most likely to occur.

Blight mainly refers to a class of diseases caused by fungi of the genus Phytophthora, which mainly cause rapid necrosis and rot of plant flowers, fruits, and leaf tissues. High humidity is the main factor affecting the occurrence and spread of diseases. Among them, melon blight and potato late blight are more important diseases.

In the prevention and control of powdery mildew, downy mildew, and blight, we mainly rely on agents such as benzimidazoles, triazoles, metalaxyl, dimethomorph, and mancozeb. Due to the long-term use of these agents, drug resistance has been produced to varying degrees, and the control effect has been reduced. At the same time, the extensive use of medicaments increases pesticide residues, endangers food safety, and aggravates environmental pollution.

Activated ester: English name acibenzolar, chemical name benzo(1,2,3)thiadiazole-7-carboxythioacid-S-methyl ester, test code CGA245704, referred to as BTH. Molecular formula C ₈ H ₆ N ₂ OS ₂ .

The activated ester is a plant disease resistance activator of benzothiadiazole carboxylate. Activated esters enable plants to acquire resistance by activating their own defense responses, thereby resisting powdery mildew, downy mildew, rust and other diseases. Suitable crops include rice, wheat, vegetables, bananas, tobacco, etc.

Combining activated esters with active ingredients of other structural types of pesticides, the synergistic composition can not only significantly improve the actual control effect, reduce the dosage and cost, but also help to avoid the occurrence of bacterial resistance and delay the development of drug resistance. The speed of production can solve the problems of resistance and cost in the current single-dose application of pesticides.

Contents of the invention

The purpose of the present invention is to provide a new bactericidal composition with low risk of resistance, low cost, good drug effect and long lasting effect.

Through a large number of indoor bioassays and field drug efficacy tests, the inventor unexpectedly found that the mixture of activated ester and pyridine fungicides in a certain ratio has a significant synergistic effect on powdery mildew, downy mildew, blight and other diseases.

The pyridine fungicide is selected from the group consisting of fluazinam, pyroxamid, boscalid, flubocalid, boscalid and the like.

The fluazinam is a broad-spectrum protective fungicide, which is very effective against Alternaria, Botrytis, Phytophthora, Plasmodium, Sclerotinia and Scrubella. English Common Name Fluazinam Chemical Name 3-chloro-N-(3-chloro-5-trifluoromethyl-2-pyridyl)-α,α,α-trifluoro-2,6-dinitro-p-toluidine Molecular formula C ₁₃ H ₄ Cl ₂ F ₆ N ₄ O ₄ . Specific diseases that fluazinam can control include cucumber gray mold, downy mildew, anthracnose, powdery mildew, tomato late blight, apple scab, leaf spot, pear black spot, rust, rice blast, sheath blight Disease, oat crown rust, grape gray mold, downy mildew, citrus scab, gray mold, potato late blight, lawn spot, etc. It also has a good effect on the pathogenic bacteria of Botrytis cinerea against benzimidazole and dicarboximide fungicides, and fluazinam is resistant to rain erosion and has a long lasting effect.

The pyrifenoxine has an English common name of pyrifenox, a chemical name of 2,4-dichloro-2-(3-pyridyl)acetophenone-O-methyl oxime, and a molecular formula of C ₁₄ H ₁₂ Cl ₂ N ₂ O. Pyrethymetaxime is a systemic fungicide with protective and therapeutic effects, which can prevent and control crop powdery mildew, leaf spot, scab and so on.

The boscalid has an English common name of Boscalid, a chemical name of 2-chloro-N-(4'-chlorobiphenyl-2-yl)nicotinamide, and a molecular formula of C ₁₈ H ₁₂ Cl ₂ N ₂ O. Boscalid transfers in plants through foliage penetration, inhibits mitochondrial succinate dehydrogenase, hinders the tricarboxylic acid cycle, interferes with the division and growth of pathogenic bacteria cells, and has protective and therapeutic effects. Inhibits spore germination, germ tube elongation, hyphal growth and sporocytic formation. Boscalid is mainly used to control powdery mildew, gray mold, various rots, brown rot and root rot.

The fluopicolide, English common name flupicolide, chemical name 2,6-dichloro-N-(3-chloro-5-trifluoromethyl-2-pyridylmethyl)benzamide, molecular formula: C ₁₄ H ₈ Cl ₃ F ₃ N ₂ O. Fluoxamid is mainly used to prevent and control oomycete diseases such as downy mildew and blight. In addition, it has certain control effects on rice blast, gray mold and powdery mildew.

The above-mentioned pyridine fungicides are all well-known fungicides in the art, and can be obtained through various commercial channels.

On the basis of the above findings, the technical solution of the present invention has been formed, namely: a fungicidal composition, the mass ratio of the activated ester (A) and one of the pyridine fungicides (B) in the composition is 100:1 ~1:100, the preferred mass ratio of A and B is 40:1~1:30. The total mass percentage of active components in the composition is 10-80%.

The composition of the present invention can be prepared into any dosage form suitable for agricultural use by known methods, and the preferred dosage forms include suspension concentrate, wettable powder, water-dispersible granule and the like. These formulations can be prepared by common methods, for example, mixing the active substance with a liquid solvent and/or a solid carrier, while adding surfactants such as emulsifiers, dispersants, stabilizers, wetting agents, and also binders, defoamers Agents, oxidizing agents, dyes, etc.

For suspending agent, usable dispersant such as polycarboxylate, pull-open powder, lignosulfonate such as one or more in lignosulfonate sodium salt, alkylnaphthalene sulfonate; emulsifier such as agricultural Milk 700# (common name alkylphenol formaldehyde resin polyoxyethylene ether), sodium dibutylnaphthalene sulfonate formaldehyde condensate, agricultural milk 2201#, Span-60# (common name sorbitan monostearate) , Nongru 1601# (common name triphenylethylphenol polyoxypropylene polyoxyethylene block polymer), one or more of TERSPERSE 4894 (2500); wetting agent such as alkylphenol polyoxyethylene ether Formaldehyde condensate phosphate, tea dry powder, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, naphthalene sulfonate, soap One or more of horn powder, silkworm excrement, sapindus powder; thickener such as one of xanthan gum, polyvinyl alcohol, bentonite, hydroxymethyl cellulose, methyl cellulose, magnesium aluminum silicate one or more; preservatives such as one or more of formaldehyde, benzoic acid, sodium benzoate; defoamers such as silicone defoamers; antifreeze agents such as ethylene glycol, propylene glycol, glycerin, urea, inorganic salts Such as one or more of sodium chloride.

For water dispersible granules, one or more of dispersants such as polycarboxylates, lignosulfonates, NNO4, and alkylnaphthalene sulfonates can be used; wetting agents such as nonylphenol polyoxyethylene ether , polyoxyethylene alcohol, alkyl sulfate, alkyl sulfonate, naphthalene sulfonate; disintegrants such as ammonium sulfate, urea, sucrose, glucose, aluminum chloride, citric acid, butanediol one or more of acid and sodium bicarbonate; binders such as one or more of diatomaceous earth, cornstarch, PVA, carboxymethyl (ethyl) cellulose; fillers such as diatomaceous earth, hydrogen carbonate One or more of calcium, light calcium carbonate, sepiolite, kaolin, white carbon black, talc, and clay.

For wettable powders, one or more of usable dispersants such as polycarboxylates, lignin sulfonates, alkylnaphthalene sulfonates; wetting agents such as alkyl sulfates, alkyl sulfonates, One or more of naphthalenesulfonate; one or more of fillers such as ammonium sulfate, urea, sucrose, glucose, diatomaceous earth, kaolin, white carbon black, light calcium, talcum powder, attapulgite, and clay .

The composition of the present invention can be provided in the form of a finished preparation, that is, the substances in the composition have been mixed; the ingredients of the composition can also be provided as a single-dose preparation, which is directly mixed in a barrel (can) before use. The concentrates of the present invention are usually mixed with water to obtain the desired concentration of active substance.

The composition of the invention can be used to prevent and treat important diseases caused by ascomycetes, basidiomycetes, oomycetes, etc., and is especially suitable for preventing and treating powdery mildew, downy mildew, blight and the like. The application site of the composition of the present invention is farmland, orchard and the like.

The composition of the present invention can also be used in combination with other compounds having herbicidal, insecticidal or bactericidal properties, especially protective fungicides, and can also be used in combination with nematicides, protective agents, growth regulators, plant nutrients or soil conditioners .

The present invention also includes a method for killing fungi on site, applying the composition of the present invention to the crops and/or fruits and the place where they are grown or stored before or after the crops and/or fruits are susceptible to disease. Application can be carried out by conventional methods, such as pouring, spraying, spraying, dusting, spreading. The application rates of the present invention vary with weather conditions or crop conditions. The duration of protection is usually related to the content of individual compounds in the composition and is related to external factors, such as climate, but the influence of climate can be mitigated by using appropriate dosage forms.

The composition of the present invention has the following advantages: first, the synergistic effect of the composition is obvious, and the drug effect is greatly improved; second, the drug effect of the composition is improved, the field dosage is reduced, the production and use costs are reduced, and pesticide residues and environmental pollution are reduced; The composition is composed of active ingredients with different action mechanisms, and the number of action sites is increased, which is beneficial to overcome and delay the emergence of drug resistance of bacteria. The fourth is to expand the bactericidal spectrum, which can prevent and control powdery mildew, downy mildew, and blight of crops at the same time.

Detailed ways

The inventor has carried out a large amount of field efficacy tests, and the results show that after the activated ester is mixed with fluazinam or acetoxime or boscalid or fluazamid or boscalid in a certain ratio, it is effective against downy mildew It has a significant synergistic effect on diseases such as mildew, powdery mildew, and blight.

The present invention is further illustrated below with specific examples, but the present invention is by no means limited to these examples. The following descriptions are only preferred embodiments of the present invention, and are only used to explain the present invention, and should not be construed as limiting the patent scope of the present invention. It should be noted that any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included within the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention should be based on the appended claims.

The percentages in all formulation ratios are mass percentages.

Suspending agent example

According to the proportion of the formula, the active ingredient, dispersant, wetting agent, thickener, antifreeze and water are mixed evenly according to the proportion of the formula, and the suspending agent is made after grinding or high-speed shearing.

Example 1: 20.2% activated ester boscalid suspension concentrate

Activated ester 20%, boscalid 0.2%, sodium methylnaphthalenesulfonate formaldehyde condensate (dispersant) 10%, sodium lauryl sulfate (wetting agent) 3%, xanthan gum (thickener) 2%, bentonite (thickener) 1%, glycerin (antifreeze) 5%, water to make up to 100%.

Embodiment 2: 40.4% activated ester·boscalid suspension concentrate

Activated ester 0.4%, boscalid 40%, polycarboxylate (dispersant) 10%, tea dry powder (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, bentonite (thickener) ) 1%, ethylene glycol (antifreeze) 5%, and water to make up to 100%.

Example 3: 30.3% activated ester·fluopyram suspension concentrate

Activated ester 30%, fluoxamid 0.3%, sodium lignosulfonate (dispersant) 10%, saponin powder (wetting agent) 3%, xanthan gum (thickener) 2%, polyethylene Alcohol (thickener) 1%, propylene glycol, (antifreeze agent) 5%, water to make up to 100%.

Example 4: 20.2% Activated Ester Fluoxamid Suspension Concentrate

0.2% activated ester, 20% fluoxamid, 10% alkylnaphthalene sulfonate (dispersant), 3% sapinberry powder (wetting agent), 2% polyvinyl alcohol (thickener), bentonite (thickener) 1%, urea (antifreeze) 5%, water to make up to 100%.

Example 5: 20.2% activated ester · fluazinam suspension concentrate

Activated ester 20%, fluazinam 0.2%, methyl naphthalene sulfonate sodium formaldehyde condensate (dispersant) 10%, alkyl sulfonate (wetting agent) 3%, polyvinyl alcohol (thickener) 2% , xanthan gum (thickener) 1%, glycerin (antifreeze agent) 5%, and water to make up to 100%.

Embodiment 6: 40.4% activated ester·fluazinam suspension concentrate

Activated ester 0.4%, fluazinam 40%, polycarboxylate (dispersant) 10%, alkyl sulfate (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, xanthan gum (thickener) Thickener) 1%, ethylene glycol (antifreeze) 5%, water to make up to 100%.

Example 7: 10% activated ester boscalid suspension concentrate

Activated ester 1%, boscalid 9%, polycarboxylate (dispersant) 10%, sodium lauryl sulfate (wetting agent) 3%, xanthan gum (thickener) 2%, bentonite ( thickener) 1%, urea (antifreeze) 5%, and water to make up to 100%.

Example 8: 10% Activated Ester · Fluoxamid Suspension Concentrate

Activated ester 2%, fluoxamid 8%, sodium methylnaphthalenesulfonate formaldehyde condensate (dispersant) 10%, sodium lauryl sulfate (wetting agent) 3%, polyvinyl alcohol (thickener ) 2%, xanthan gum (thickener) 1%, sodium chloride (antifreeze) 5%, and water to make up to 100%.

Example 9: 10% activated ester · fluazinam suspension concentrate

Activated ester 5%, fluazinam 5%, polycarboxylate (dispersant) 10%, sodium lauryl sulfate (wetting agent) 3%, xanthan gum (thickener) 2%, bentonite (thickening agent) Thickener) 1%, urea (antifreeze) 5%, water to make up to 100%.

Example 10: 41% activated ester · boscalid suspension concentrate

Activated ester 40%, boscalid 1%, polycarboxylate (dispersant) 10%, tea dry powder (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, bentonite (thickener) ) 1%, propylene glycol (antifreeze) 5%, and water to make up to 100%.

Example 11: 15.5% Activated Ester · Fluoxamid Suspension Concentrate

Activated ester 0.5%, Fluoxamid 15%, Polycarboxylate (dispersant) 10%, Chaku powder (wetting agent) 3%, Polyvinyl alcohol (thickener) 2%, Bentonite (thickener) agent) 1%, glycerin (antifreeze) 5%, and water to make up to 100%.

Example 12: 20.5% activated ester · fluazinam suspension concentrate

Activated ester 20%, fluazinam 0.5%, polycarboxylate (dispersant) 10%, tea dry powder (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, bentonite (thickener) 1%, propylene glycol (antifreeze) 5%, water to make up to 100%.

Example 13: 30% activated ester·pyridoxine suspension concentrate

15% activated ester, 15% pyridoxine, 10% polycarboxylate (dispersant), 3% tea dry powder (wetting agent), 2% polyvinyl alcohol (thickener), bentonite (thickener) 1%, propylene glycol (antifreeze) 5%, water to make up to 100%.

Example 14: 20% activated ester · acetochlor suspension concentrate

Activated ester 5%, pyraclostrobin 15%, polycarboxylate (dispersant) 10%, tea dry powder (wetting agent) 3%, polyvinyl alcohol (thickener) 2%, bentonite (thickener) 1%, glycerin (antifreeze) 5%, water to make up to 100%.

Wettable powder example

The pesticide active components, auxiliary agents and fillers are mixed in proportion, air-jet pulverized and then mixed to obtain wettable powder. Main equipment: mixer, jet mill.

Example 15: 80% activated ester boscalid wettable powder

Activated ester 50%, boscalid 30%, sodium lauryl sulfate (wetting agent) 2%, sodium lignosulfonate (dispersant) 3%, white carbon black 10% (filler), kaolin to make up to 100 %.

Example 16: 80% activated ester·fluopyram wettable powder

Activated ester 30%, fluoxamid 50%, sodium lauryl sulfate (wetting agent) 2%, sodium lignosulfonate (dispersant) 3%, white carbon black 10% (filler), kaolin supplemented to 100%.

Example 17: 80% activated ester·fluazinam wettable powder

Activated ester 45%, fluazinam 35%, sodium lauryl sulfate (wetting agent) 2%, sodium lignosulfonate (dispersant) 3%, white carbon black 10% (filler), pottery clay to make up to 100% .

Example 18: 31% activated ester boscalid wettable powder

Activated ester 1%, boscalid 30%, alkyl sulfate (wetting agent) 3%, polycarboxylate (dispersant) 3%, white carbon black (filler) 11%, bentonite to 100%.

Example 19: 41% activated ester·fluopyram wettable powder

40% of activated ester, 1% of fluoxamid, 3% of alkyl sulfate (wetting agent), 3% of polycarboxylate (dispersant), 11% of kaolin (filler), and 100% of attapulgite.

Example 20: 31% activated ester·fluazinam wettable powder

Activated ester 1%, fluazinam 30%, alkyl sulfonate (wetting agent) 3%, sodium lignosulfonate (dispersant) 3%, white carbon black (filler) 11%, pottery clay to make up to 100%.

Example 21: 35% activated ester·pyridoxine wettable powder

30% of activated ester, 5% of pyridoxine, 3% of alkyl sulfate (wetting agent), 3% of polycarboxylate (dispersant), 11% of white carbon black (filler), and 100% of bentonite.

Example 22: 50% activated ester · acetochlor wettable powder

10% activated ester, 40% pyraclostrobin, 3% alkyl sulfate (wetting agent), 3% polycarboxylate (dispersant), 11% kaolin (filler), and attapulgite to make up to 100%.

Examples of water dispersible granules

According to the proportion of the formula, the original drug, powdery carrier, wet spreading agent and binder are mixed and pulverized, and after adding water and kneading, they are added to a granulator equipped with a certain size screen for granulation. Then dry and sieve (according to the range of the sieve) to obtain granular products.

Example 23: 30% activated ester boscalid water dispersible granules

Activated ester 5%, boscalid 25%, polycarboxylate (dispersant) 5%, sodium lignosulfonate (dispersant) 7%, sodium lauryl sulfate (wetting agent) 2%, ammonium sulfate (Disintegrant) 5%, supplemented to 100% with light calcium carbonate.

Example 24: 40% activated ester·fluopyram water dispersible granules

Activated ester 10%, fluoxamid 30%, sodium alkylnaphthalene sulfonate (dispersant) 5%, sodium lignosulfonate (dispersant) 7%, sodium lauryl sulfate (wetting agent) 2% , urea (disintegrant) 5%, light calcium carbonate supplemented to 100%.

Example 25: 50% activated ester·fluazinam water dispersible granules

Activated ester 40%, fluazinam 10%, sodium alkylnaphthalene sulfonate (dispersant) 5%, sodium lignosulfonate (dispersant) 7%, sodium lauryl sulfate (wetting agent) 2%, glucose (Disintegrant) 5%, supplemented to 100% with light calcium carbonate.

Example 26: 20% activated ester·fluopyram water dispersible granules

Activated ester 2%, Fluoxamid 18%, Polycarboxylate (dispersant) 5%, Sodium lignosulfonate (dispersant) 7%, Sodium lauryl sulfate (wetting agent) 2%, Sulfuric acid Ammonium (disintegrant) 5%, light calcium carbonate supplemented to 100%.

Example 27: 35% activated ester·pyridoxine water dispersible granules

Activated ester 25%, Pyridoxine 10%, Sodium alkylnaphthalene sulfonate (dispersant) 5%, Sodium lignosulfonate (dispersant) 7%, Sodium lauryl sulfate (wetting agent) 2%, Urea (Disintegrant) 5%, supplemented to 100% with light calcium carbonate.

Example 28: 25% activated ester·baxachlor water dispersible granules

Activated ester 10%, pyraclostrobin 15%, sodium alkylnaphthalene sulfonate (dispersant) 5%, sodium lignosulfonate (dispersant) 7%, sodium lauryl sulfate (wetting agent) 2%, glucose (Disintegrant) 5%, supplemented to 100% with light calcium carbonate.

Field application example 1: Field efficacy test of activated esters combined with boscalid against cucumber powdery mildew

Select plots with severe incidence of cucumber powdery mildew all year round. See Table 1 for the test agents and their dosages. Each treatment was repeated in 4 plots, each with an area of 20 square meters. The first spraying was carried out at the early stage of disease occurrence, sprayed evenly, and sprayed until the leaves dripped, and the clean water treatment was used as the blank control. Apply the second medicine after 7 days. Investigate the incidence of cucumber powdery mildew before the first medicine and 7 days after the second medicine. Five-point sampling was adopted in each plot, and two plants were surveyed at each point, and all leaves were surveyed for each plant, and the percentage of diseased spot area to the entire leaf area was used to classify and record. The disease index and control effect of each treatment were calculated.

Grading standards:

Grade 0: no lesions;

Grade 1: Lesion area accounts for less than 5% of the entire leaf area;

Grade 3: The lesion area accounts for 6%-15% of the entire leaf area;

Grade 5: The lesion area accounts for 16%-25% of the entire leaf area;

Grade 7: The lesion area accounts for 26%-50% of the entire leaf area;

Grade 9: The lesion area accounts for more than 50% of the entire leaf area.

Drug efficacy calculation method:

It can be seen from Table 1 that the control effects of activated esters and boscalid single agents on cucumber powdery mildew are 74.76% and 77.86% respectively, while the fungicidal compositions of the embodiments of the present invention have significantly improved control effects, and the lowest control effect The efficiency is 86.02%, and the highest reaches 91.34%. The results of field experiments fully show that the combination of activated ester and boscalid has a significant synergistic effect on cucumber powdery mildew, and the control effect is greatly improved when the dosage of active ingredients per mu is reduced compared with that of a single agent. Field investigations have found that the composition can also treat cucumber downy mildew. Therefore, the composition of the invention has the beneficial effects of reducing costs, delaying resistance, reducing application times and pesticide residues, and expanding control spectrum.

Table 1 Field test results of activated esters and boscalid against cucumber powdery mildew

Test drug Dosage (g·a.i./ha) Predrug condition index 7 days after the second dose of the disease index Anti-efficacy (%) 50% activated ester wettable powder 50 3.7 11.9 74.76 50% Boscalid water dispersible granules 300 2.8 7.9 77.86 Formulation Example 1 (20.2% activated ester boscalid suspension concentrate) 45 3.2 5.7 86.02 Formulation Example 2 (40.4% activated ester boscalid suspension concentrate) 150 3.8 6.1 87.40 Formulation Example 7 (10% activated ester boscalid suspension) 48 3.5 5.3 88.12 Formulation Example 10 (41% activated ester boscalid suspension concentrate) 42 2.9 3.2 91.34 Formulation Example 15 (80% activated ester boscalid wettable powder) 45 3.6 6.2 86.48

Test drug Dosage (g·a.i./ha) Predrug condition index 7 days after the second dose of the disease index Anti-efficacy (%) Formulation Example 18 (31% activated ester boscalid wettable powder) 48 2.7 4.3 87.50 Formulation Example 23 (30% activated ester boscalid water dispersible granules) 48 4.2 7.3 86.36 CK - 3.1 39.5 -

Field Application Example 2: Field Efficacy Test of Activated Ester and Fluoxamid on Tobacco Downy Mildew

Select the plot where tobacco downy mildew first occurred. See Table 2 for the test agents and their dosages. Each treatment was repeated in 4 plots, each with an area of 20 square meters. The first spraying was carried out at the early stage of the disease, and the whole plant was sprayed evenly until the leaves dripped. The clean water treatment was used as the blank control. Apply the second medicine after 7 days. Investigate the incidence of tobacco downy mildew before the first medicine and 7 days after the second medicine. Five-point sampling was adopted in each plot, and 2 plants were investigated at each point, and all leaves were investigated for each plant, and the percentage of diseased spot area to the entire leaf area was used to classify and record. The disease index and control effect of each treatment were calculated.

Grading standards:

Grade 0: no lesions;

Grade 1: Lesion area accounts for less than 5% of the entire leaf area;

Grade 3: The lesion area accounts for 6%-15% of the entire leaf area;

Grade 5: The lesion area accounts for 16%-25% of the entire leaf area;

Grade 7: The lesion area accounts for 26%-50% of the entire leaf area;

Grade 9: The lesion area accounts for more than 50% of the entire leaf area.

Drug efficacy calculation method:

As can be seen from Table 2: the control effects of activated esters and fluoxamid single agents on tobacco downy mildew are 73.49% and 78.66% respectively, and the fungicidal compositions of the embodiments of the present invention have significantly improved control effects. The minimum control efficiency is 85.72%, and the highest is 91.29%. The results of field tests fully show that the combination of activated esters and fluoxamid has a significant synergistic effect on tobacco downy mildew, and the control effect is greatly improved when the amount of active ingredients per mu is reduced. Field investigations have found that the composition can also treat tobacco powdery mildew. Therefore, the composition of the present invention has the beneficial effects of reducing costs, delaying resistance, reducing application times and pesticide residues.

Table 2 Field test results on tobacco downy mildew compounded by activated esters and fluoxamid

Test drug Dosage (g·a.i./ha) predrug condition index 7 days after the second dose of the disease index Anti-efficacy (%) 50% activated ester wettable powder 50 3.5 11.5 73.49 25% Fluoxamid Suspension Concentrate 250 3.1 8.2 78.66 Formulation Example 3 (30.3% activated ester · fluoxamid suspension concentrate) 45 2.5 4.3 86.12 Formulation Example 4 (20.2% activated ester · fluoxamid suspension concentrate) 140 3.9 6.9 85.72 Formulation Example 8 (10% activated ester · fluoxamid suspension concentrate) 48 3.4 5.1 87.89 Formulation Example 11 (15.5% activated ester · fluoxamid suspension concentrate) 48 3.2 5.5 86.13 Formulation Example 16 (80% activated ester Fluoxamid wettable powder) 45 2.6 4.3 86.65 Formulation Example 19 (41% Activated Ester Fluoxamid WP) 42 3.8 4.1 91.29 Formulation Example 24 (40% activated esters Fluoxamid water dispersible granules) 45 2.7 4.3 87.15

Test drug Dosage (g·a.i./ha) predrug condition index 7 days after the second dose of the disease index Anti-efficacy (%) CK - 2.8 34.7 -

Field Application Example 3: Field Efficacy Test of Activated Ester and Fluazinam on Capsicum Blight

Select the plot where pepper blight first occurred. See Table 2 for the test agents and their dosages. Each treatment was repeated in 4 plots, each with an area of 20 square meters. The first spraying was carried out at the early stage of the disease, and the whole plant was sprayed evenly until the leaves dripped. The clean water treatment was used as the blank control. Apply the second medicine after 7 days. The incidence of pepper blight was investigated before the first medicine and 7 days after the second medicine. Five-point sampling was adopted in each plot, and 2 plants were investigated at each point, and all leaves were investigated for each plant, and the percentage of diseased spot area to the entire leaf area was used to classify and record. The disease index and control effect of each treatment were calculated.

Grading standards:

Grade 0: no lesion;

Grade 1: Lesion area accounts for less than 5% of the entire leaf area;

Grade 3: The lesion area accounts for 6%-15% of the entire leaf area;

Grade 5: The lesion area accounts for 16%-25% of the entire leaf area;

Grade 7: The lesion area accounts for 26%-50% of the entire leaf area;

Grade 9: The lesion area accounts for more than 50% of the entire leaf area.

Drug efficacy calculation method:

As can be seen from Table 3: the control effects of activated ester and fluazinam single agent on capsicum blight were 73.21% and 77.03% respectively, and the fungicidal composition of the embodiment of the present invention had a significant improvement in the control effect, and the minimum control effect was 85.77%, the highest reached 92.33%. The results of field experiments fully show that the combination of activated ester and fluazinam has a significant synergistic effect on pepper blight, and the control effect is greatly improved when the amount of active ingredients per mu is reduced. Field investigations have found that the composition can also treat pepper powdery mildew. Therefore, the composition of the present invention has the beneficial effects of reducing costs, delaying resistance, reducing application times and pesticide residues.

Table 3 Field test results of activated esters combined with fluazinam against pepper blight

Test drug Dosage (g·a.i./ha) predrug condition index 7 days after the second dose of the disease index Anti-efficacy (%) 50% activated ester wettable powder 50 2.1 8.5 73.21 500 g/L fluazinam suspension concentrate 250 1.7 5.9 77.03 Formulation Example 5 (20.2% activated ester fluazinam suspension concentrate) 45 2.5 4.8 87.29 Formulation Example 6 (40.4% Activated Ester Fluazinam Suspension Concentrate) 150 2.3 4.7 86.47 Formulation Example 9 (10% activated ester fluazinam suspension concentrate) 48 1.6 3.1 87.17 Formulation Example 12 (20.5% activated ester fluazinam suspension concentrate) 42 1.9 2.2 92.33 Formulation Example 17 (80% activated ester fluazinam wettable powder) 45 2.7 5.5 86.52 Formulation Example 20 (31% activated ester fluazinam wettable powder) 48 2.0 4.3 85.77 Formulation Example 25 (50% activated ester fluazinam water dispersible granules) 45 2.8 5.0 88.18 CK - 1.8 27.2 -

Claims (7)

1. bactericidal composition, it is characterized in that: active ingredient is Acibenzolar (A) and a kind of pyridines bactericide (B).

2. bactericidal composition according to claim 1 is characterized in that: preferred 100: 1～1: 100 of the mass ratio of effective constituents A and B.

3. bactericidal composition according to claim 2 is characterized in that: preferred 40: 1～1: 30 of the mass ratio of effective constituents A and B.

4. according to each described bactericidal composition of claim 1-3, it is characterized in that: described pyridines bactericide is selected from fluazinam, pyrifenox, Boscalid, fluorine Boscalid, pyridine bacterium amine.

5. according to each described bactericidal composition of claim 1-3, it is characterized in that: active component quality percentage composition is 10～80% in the composition.

6. bactericidal composition according to claim 1 is characterized in that: said composition can be mixed with suspending agent, wetting powder, water dispersible granules.

7. the described bactericidal composition of claim 1 is used to prevent and treat the disease on cereal, vegetables, fruit tree, the flowers.