CN102671237B - High-simulation tissue engineering nerve-repair material and preparation method - Google Patents
High-simulation tissue engineering nerve-repair material and preparation method Download PDFInfo
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Abstract
The invention relates to a high-simulation tissue engineering nerve-repair material and a preparation method. The high-simulation tissue engineering nerve-repair material is characterized by being prepared by one or two or all of I-type collagen, chitosan and gelatin. The material comprises, by weight, I-type collagen 1-10 parts and gelatin 1 part, can be used for basic research on nerve injury repair and bridging repair of injured or defected clinical human body spinal cord and peripheral nerves, facilitates growth of nerve regeneration fibers and can be used for repair of the injured spinal cord and the peripheral nerves.
Description
Technical field
The present invention relates to the multiple biomaterials such as collagen, gelatin and chitosan with and processing technique and manufacture method, be a kind of high-artificial tissue engineering nerve repair material and preparation method thereof exactly.
Background technology
The reparation of nerve injury is a global problem.In recent years, application tissue engineering technique is prepared the focus that renovating bracket material assembling organizational project substitute repairing nerve damage becomes research, and its research emphasis comprises: the bio-imitability of (1) artificial graft's timbering material microstructure and the histocompatibility of material, cell compatibility and degradability research; (2) use in conjunction of support and extracellular matrix and neurotrophic factor; (3) introducing of seed cell.Its medium-height trestle is as the carrier of seed cell and neurotrophic factor, and as the main body that forms repairing of neural injury microenvironment, it builds and improvement in performance research becomes the key that restriction peripheral nerve injury is repaired.
Bio-derived material has good biocompatibility, is one of at present more satisfactory support raw material, mainly contains collagen protein, fibrin, chitin, chitosan and cellulose derivative etc.Collagen (collagen) is the major structural protein matter of body cell epimatrix (extracellularmatrix, ECM), distributes the widest at human body, accounts for 30%, has 14 kinds more than.NTx is that in various collagen, content is maximum, and it is provided for maintaining the support of structure for biological cell.NTx is comprised of three α chains, and the fibril of the triple-helix structure that to form diameter be 67nm, is the key component of basic mode, has resistance to compression and maintains the function of space structure, is mainly distributed in bone cornea, and tendon etc. are located.It is a kind of good surfactant, and demonstrates the good penetrance without fat interface, biodegradability, poor antigen and superior biocompatibility, is easy to be absorbed, and does not produce inflammatory reaction, is biomaterial conventional in organizational project; Gelatin (Gelatin) is a kind ofly by tropocollagen molecule, to be degraded and the mixtures of polypeptides that makes, has 18 seed amino acids and has formed gelatin peptide chain, and collagen is extensively present in bone, tendon and the connective tissue of animal, can be by bio-enzyme degradation.A large amount of experiments show: the neural tissue engineering material made from extracellular matrix not only has good biocompatibility, also have good biologic activity simultaneously, are conducive to delay and the growth of adhesion, propagation and the regenerated fiber of neurocyte.Chitosan (Chitosan) is a kind of natural polysaccharide, and the amido with certain amount, is dissolved in acid medium, and is with certain positive charge.Its wide material sources, cheap, there is good biology performance, can not cause allergic reaction and rejection, can progressively degrade in vivo, there is good biocompatibility and biodegradability.Chitosan has antibacterial activity, bio-adhesive effect, flocculation and active anticancer; Can be processed into multi-form, as powder, membrane, paste, fiber etc.Just because of these character, chitosan is attracted increasing attention in the application aspect biomedical and pharmaceutics.
Nervous tissue's regeneration is different from other tissue, and neuranagenesis fiber is oriented growth in a certain direction, just likely effectively repairs damage.There are some researches show that the bionical degree of bridge joint repair materials internal structure has determined to a great extent growth, the migration results of neuranagenesis cell, and the stretching, extension of regenerating nerve fiber and neural axon growth, connection etc. are all played an important role.At present, the internal structure of the more neural transplantation material of application and research mainly comprises that large empty tubular structure, blank pipe add content gel-like structure and parallel longitudinal tubule (L tubule) shape structure.Although these structures to a certain extent all can the neural broken ends of fractured bone of bridge joint two, provide a relatively isolated microenvironment for neuranagenesis, but material internal does not have directed, regular hole and structure of arranging, be unfavorable for the growth of neural axon, the repairing effect of nerve injury is relatively poor.
Desirable tissue engineering bracket material must have the dummy feature of the microtubule matrix arrangement of axle orientation, so just can make the seed cell of transplanting can be along microtubule wall axially-aligned, migration, and be conducive to Regenerating Axons oriented growth, thereby make to greatest extent the nerve of damage obtain effective reparative regeneration.But because previously compound bridge joint carrier material internal structure is all without certain rule (in pore size and direction), cannot keep desirable physical arrangement in vivo, be unfavorable for spinal cord or peripheral nerve sections damage regenerated fiber oriented growth, thereby affected the effect for the treatment of or experiment.Preparation has the collagen-chitin bridge material that axle orientation microtubule matrix is arranged simulation architecture feature, there is no in the world at present similar report.
Summary of the invention
One of object of the present invention is to provide a kind of high-artificial tissue engineering nerve repair material, this material has axle orientation matrix and arranges the simulation architecture that between microtubule and microtubule, extensively hole is linked up, microtubule pore size and direction can regulate and control as required, and there is good biocompatibility and controllable biological degradability, it both can be used for the basic research of repairing of neural injury, also can be used for clinical human body spinal cord and peripheral nerve injury or damaged bridge joint reparation.Be conducive to the growth of neuranagenesis fiber, can be used for the reparation of spinal cord, peripheral nerve injury.
Another object of the present invention is to provide a kind of preparation method of high-artificial tissue engineering nerve repair material.
To achieve these goals, the technical solution adopted in the present invention is: a kind of high-artificial tissue engineering nerve repair material, is characterized in that: described nerve repair material is to make according to the raw material of following portions by weight: type i collagen albumen 1-10 part, 1 part, gelatin; Its preparation method carries out according to the following steps:
1) by above-mentioned parts by weight, taking concentration is that the type i collagen albumen of 28mg/ml is that 3mg/ml acetum dissolves 24 hours by putting into concentration
2) in 4 ℃ of isoperibols, with 15000r/min mixing speed, stir 90min, make suspension;
3) take 1 part, gelatin, add in 3mg/ml acetum and dissolve 24 hours;
4), under 4 ℃ of constant temperatures, with 15000r/min mixing speed, stir 90min and make suspension;
5) blend step 2), 4) two kinds of suspensions keep 4 ℃ of constant temperature, then stir 90min with 15000r/min mixing speed, make the suspension of collagen protein and gelatin, standing 12 hours of evacuation;
6) by sealing both ends in the suspension Implanted Silicon sebific duct of the step 5) preparing, along tube axial direction, slowly put into profound hypothermia condensing agent liquid nitrogen, it is 10 * 10 that admission velocity is controlled
-7ms
-1to 10 * 10
-5ms
-1;
7) suspension-silica gel tube frozen material is put into the aluminum dish that pre-cooling is good, in-60 ℃, 100mtorr condition, lyophilizing is 24 hours;
8) under vacuum state, be warming up to 0 ℃ and keep 6h, then continue to be warming up to 22 ℃ of maintenance 30～60min, remove vacuum, rise to room temperature;
9) material is soaked to 48h with 1wt% genipin crosslinked, distilled water is dialysed repeatedly;
10) by material aseptic sealed packages and be placed in 20kGyCO60 environment illumination-based disinfection 24 hours.It is relative closure structure (thickness: 2.45 ± 1.43 μ m) that the novel spinal cord that the present invention develops, peripheral nerve reparation bridge material have the 1. outer surface of material; 2. the microtubule pore size of material can be controlled in 20 μ m～200 μ m; 3. the direction of travel of microtubule is axial and parallel, even; 4. between microtubule, there is a large amount of micropores (pore size: 20.68 ± 4.61 μ m) of communicating with each other.Timbering material aperture is controlled, can adapt to the difference needs of different condition: wide-aperture material is creeped to the attached wall of cell or poured into as a support carrier function such as Olfactory essheathing cell, schwann cell; The material of small-bore is suitable for compound various nerve growth factor, medicine etc.Axially matrix is arranged microtubule simulation architecture, is conducive to the extension of directional guide regenerating nerve cell and nerve fiber growth, is applicable to the bridge joint reparation at spinal cord and peripheral nerve defection place.Between microtubule, extensively hole is linked up, and is conducive to cell migration and nutrient substance transportation exchange, utilizes neuranagenesis.The material surface relative closure of developing, makes can prevent that hypertrophy connective fiber from growing into damaged section implanting spinal cord or peripheral nerve defection place, avoids hindering the oriented growth of neuranagenesis aixs cylinder.
The inside pore size that material of the present invention has a material under identical conditions evenly, microtubule axially parallel arranges the extensively structural advantages such as communication of height emulation, contiguous microtubule hole, in neural repair tissue engineering, to more be conducive to spinal cord and peripheral nerve regeneration fiber microtubule oriented growth and migration vertically, be expected to obtain spinal cord and perineural effective reparation, regeneration, in clinical and experiment, have a extensive future, act on huge.
The high-artificial tissue engineering nerve injury repairing material that the present invention proposes has following characteristics:
1. adopted the better raw material of biocompatibility, the internal milieu of more approaching biology, is conducive to neural regeneration more.Wherein type i collagen albumen (collagenI) is the key component of extracellular matrix; the adhesion, migration and the propagation that are conducive to cell; can promote neural regeneration, gelatin (gelatin) is that organizational project is repaired the most frequently used raw material, is the optimal carrier of neurotrophic factor;
2. optimum mixture ratio example and the impact at aspects such as biological property and mechanical properties on material of the biomaterials such as type i collagen albumen (collagenI), chitosan (chitosan), gelatin (gelatin) have been verified;
3. verified relation between temperature, speed and additive (acetic acid) concentration and new type nerve organizational project repair materials internal structure;
4. verify various composition different proportions and mixed the impact on material internal structure;
5. verify crosslinked between component, curing optimum condition has increased material tensile strength and rigidity, better improved Mechanical Properties of Materials;
6. by controlling composition component ratio and the crosslinked parameter of material, effectively reduce material decomposition rate in vivo.
High-artificial tissue engineering nerve injury repairing material of the present invention is that a kind of novel spinal cord, peripheral nerve are repaired bridge material, both can directly implant spinal cord, peripheral nerve defection place; Also can be used as the carrier that each seed cell of neural tissue engineering is transplanted, can be compound, the medicine that carries various promotion neuranagenesis, for human body spinal cord, the damage of peripheral nerve sections, bridge joint after damaged; Can be used for equally the basic research of spinal cord, peripheral nerve sections injury repairing.
Accompanying drawing explanation
Fig. 1 is the picture that under scanning electron microscope, axial slices is observed, and its material internal is the axial microtubule of emulation (scale=200 μ m) that moves towards parallel, aperture homogeneous, arrangement regulation;
Fig. 2 is the picture that under scanning electron microscope, axial slices is observed, and has microcellular structure widely communicate with each other (scale=50 μ m) between the microtubule that display material inner shaft is arranged to matrix;
Fig. 3 is the picture under the Electronic Speculum of cross section, the outside relative closure structure (scale=200 μ m) of display material;
Fig. 4 is the picture under the Electronic Speculum of cross section, and display material internal capillary internal diameter is homogeneous (scale=200 μ m) comparatively.
The specific embodiment
Below in conjunction with processing technology and embodiment, the present invention is described in further details.It should be noted that the present embodiment, only for the present invention is described, does not limit practical range of the present invention.
According to technical scheme of the present invention, this high-artificial tissue engineering nerve injury repairing material is mixed with collagen and chitosan, and material adopts type i collagen albumen (collagenI), chitosan (chitosan), gelatin (gelatin).Its formula proportion is: (280:28:28)～and (280:280:280)
Above-mentioned material is in cryogenic temperature gradient environment, adjust stranguria of cold type speed and additive (acetic acid) concentration change and the thermograde rate of regression of raw mixture, it is the key factor that determines material internal microcellular structure pore size, micropore orientation, change and to be marked with speed that collagen-chitosan sugar mixture silica gel tube enters stranguria of cold type agent and can to reach the material of required preparation pore size and hole direction and uniformity coefficient in internal structure are all had to Modulatory character, thereby obtain high emulation spinal cord, peripheral nerve tissue's engineering injury repairing material.
Embodiment 1:(is according to type i collagen albumen: chitosan: the ratio of gelatin is 280:70:70):
According to technical scheme of the present invention, take collagen protein, gelatin and chitosan as example, its manufacture method is carried out according to the following steps:
1). take by weight the type i collagen albumen of aforementioned proportion, by the concentration of 28mg/ml, put into 3mg/ml acetum and dissolve 24 hours;
2). in 4 ℃ of isoperibols, 15000r/min stirs 90min, makes suspension;
3). the chitosan, the gelatin that separately by ratio, take above-mentioned part by weight add in 3mg/ml acetum and dissolve 24 hours;
4). at 4 ℃ of constant temperature, 15000r/min stirs 90min and makes suspension;
5). mix two kinds of suspensions and keep 4 ℃ of constant temperature, 15000r/min stirs 90min, makes the suspension of collagen protein and chitosan, standing 12 hours of evacuation;
6). it is that 2mm, external diameter are the silica gel tube sealing both ends of 3mm, long 5cm to 20cm that the bridge material suspension preparing is injected to internal diameter, along tube axial direction, slowly puts into the agent of profound hypothermia stranguria of cold type, and it is 10 * 10 that admission velocity is controlled
-7ms
-1to 10 * 10
-5ms
-1;
7). suspension-silica gel tube frozen material is put into the aluminum dish that pre-cooling is good, lyophilizing 24h in-60 ℃, 100mtorr condition;
8). under vacuum state, be warming up to 0 ℃ and keep 6h, then continue to be warming up to 22 ℃ of maintenance 30～60min, remove vacuum, rise to room temperature;
9). material is soaked to 48h with 1wt% genipin crosslinked, distilled water is dialysed repeatedly;
10). by material aseptic sealed packages and be placed in 20kGyCO
60in environment, illumination-based disinfection is 24 hours.
The bridge material that the present invention develops has following characteristics: 1. the outer surface of material is relative closure structure, can effectively stop scar tissue grow into (Fig. 3); 2. the micro-pore diameter of material can artificially simply regulate and control, and has both been conducive to growing into of neuranagenesis fiber, is conducive to again the implantation of neural factor and seed cell; 3. internal micro-tubes size uniform, is axial matrix and arranges, and with the emulation of normal neural basement membrane structure height, is conducive to the oriented growth (Fig. 1, Fig. 4) of neuranagenesis fiber; 4. between microtubule, extensively hole is linked up, and is conducive to cell migration and nutrient substance transportation exchange, utilizes neuranagenesis (Fig. 2); 5. zoopery proves the good and controlled neuranagenesis that is conducive to of degradation speed of this Biocompatibility; 6. after this material through special technology is processed, mechanical property is good, and tension and non-deformability are good.
Following instance 2～8 each raw-material mixing ratios are identical.
Embodiment 2: adopt collagen (I type), chitosan and gelatin as raw material, its weight is respectively 280mg, 280mg, 280mg, and implementation process is with example 1.
Embodiment 3: adopt collagen (I type), chitosan and gelatin as raw material, its weight is respectively 280mg, 28mg, 28mg, and implementation process is with example 1.
Embodiment 4: adopt collagen (I type) and chitosan as raw material, its weight is respectively 280mg, 280mg, and implementation process is with example 1.
Embodiment 5: adopt collagen (I type) and chitosan as raw material, its weight is respectively 280mg, 28mg, and implementation process is with example 1.
Embodiment 6: adopt collagen (I type) and chitosan as raw material, its weight is respectively 280mg, 70mg, and implementation process is with example 1.
Embodiment 7: adopt collagen (I type) and gelatin as raw material, its weight is respectively 280mg, 280mg, and implementation process is with example 1.
Embodiment 8: adopt collagen (I type) and gelatin as raw material, its weight is respectively 280mg, 28mg, and implementation process is with example 1.
Embodiment 9: adopt collagen (I type) and gelatin as raw material, its weight is respectively 280mg, 70mg, and implementation process is with example 1.
Embodiment 10: adopt chitosan and gelatin as raw material, its weight is respectively 70mg, 70mg, and implementation process is with example 1; Its preparation process has been omitted step 1), 2).
Embodiment 11: adopt collagen (I type) as raw material, its weight is 280mg, and implementation process is with example 1; Its preparation process has been omitted step 3), 4), 5).
Embodiment 12: adopt collagen (I type) as raw material, its weight is 28mg, and implementation process is with example 1; Its preparation process has been omitted step 3), 4), 5).
Embodiment 13: adopt collagen (I type) as raw material, its weight is 70mg, and implementation process is with example 1; Its preparation process has been omitted step 3), 4), 5).
Embodiment 14: adopt chitosan as raw material, its weight is 70mg, and implementation process is with example 1; Its preparation process has been omitted step 1), 2).
Embodiment 15: adopt gelatin as raw material, its weight is 70mg, and implementation process is with example 1; Its preparation process has been omitted step 1), 2).
The novel high emulation neural tissue engineering repair materials that the present invention develops has internal diameter size microcellular structure between the aperture of the various sizes between 20～200 μ m and the Y-direction microtubule of parallel, uniform single axle orientation and the pipe of extensively linking up that can simple and easy regulation and control.This material also can be made different external forms easily, as column type, and elliptic cylinder etc.In the application of bioengineered tissue material, larger aperture the attached wall of cell is creeped or is poured into and play support, carrier function as Olfactory essheathing cell, schwann cell etc.; Smaller aperture due be suitable for compound various nerve growth factor, medicine etc., as the carrier of its slow release, for the bridge joint at the damaged place of spinal cord or peripheral nerve tissue.The material that the present invention the develops micro-tubular structure no matter inner shaft is arranged to matrix, or surperficial relative closure structure, all close to normal nervous tissue structure, make to implant the damaged place of spinal cord or peripheral nerve tissue, can prevent that the hypertrophy connective tissue defect of growing into from causing neuranagenesis to hinder, the effective oriented growth of physical guide regenerating nerve fiber is supported regenerating nerve histotrophic nutrition mass exchange and metabolism simultaneously again.Be widely used in the clinical treatment of human peripheral nerve or segments of spinal cord damage and the experimentation of animal peripheral nerve or segments of spinal cord damage.
Claims (1)
1. a high-artificial tissue engineering nerve repair material, is characterized in that: described nerve repair material is to make according to the raw material of following portions by weight: type i collagen albumen 1-10 part, 1 part, gelatin; Its preparation method carries out according to the following steps:
1) by above-mentioned parts by weight, taking concentration is that the type i collagen albumen of 28mg/ml is that 3mg/ml acetum dissolves 24 hours by putting into concentration;
2) in 4 ℃ of isoperibols, with 15000r/min mixing speed, stir 90min, make suspension;
3) take 1 part, gelatin, add in 3mg/ml acetum and dissolve 24 hours;
4), under 4 ℃ of constant temperatures, with 15000r/min mixing speed, stir 90min and make suspension;
5) blend step 2), 4) two kinds of suspensions keep 4 ℃ of constant temperature, then stir 90min with 15000r/min mixing speed, make the suspension of collagen protein and gelatin, standing 12 hours of evacuation;
6) by sealing both ends in the suspension Implanted Silicon sebific duct of the step 5) preparing, along tube axial direction, slowly put into profound hypothermia condensing agent liquid nitrogen, it is 10 * 10 that admission velocity is controlled
-7ms
-1to 10 * 10
-5ms
-1;
7) suspension-silica gel tube frozen material is put into the aluminum dish that pre-cooling is good, in-60 ℃, 100mtorr condition, lyophilizing is 24 hours;
8) under vacuum state, be warming up to 0 ℃ and keep 6h, then continue to be warming up to 22 ℃ of maintenance 30～60min, remove vacuum, rise to room temperature;
9) material is soaked to 48h with 1wt% genipin crosslinked, distilled water is dialysed repeatedly;
10) by material aseptic sealed packages and be placed in 20kGyCO60 environment illumination-based disinfection 24 hours.
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| CN103263308B (en) * | 2013-05-17 | 2015-07-29 | 中国人民解放军第四军医大学 | Many micropores degradable collagen-chitin nerve trachea and preparation method thereof |
| CN106581754A (en) * | 2016-12-30 | 2017-04-26 | 沈阳尚贤微创医疗器械股份有限公司 | High-simulation tissue engineering nerve repairing scaffold and making method thereof |
| CN107551317B (en) * | 2017-07-11 | 2020-05-08 | 华南师范大学 | Colloidal crystal bionic lung tissue engineering scaffold for orderly promoting growth of lung cells and preparation method and application thereof |
| CN110101918B (en) * | 2019-05-24 | 2021-09-21 | 上海市同济医院 | Hierarchical pore functional scaffold material for mobilizing endogenous neural stem cells to repair spinal cord injury and preparation method and application thereof |
| CN112245659B (en) * | 2020-09-10 | 2021-11-30 | 温州医科大学 | Preparation method and application of composite stent for directionally guiding optic nerve axon regeneration |
| CN112891624A (en) * | 2021-03-19 | 2021-06-04 | 潍坊奥精医学研究有限公司 | Preparation method of spinal cord regeneration and repair material |
| CN113274548B (en) * | 2021-06-23 | 2022-07-05 | 右江民族医学院附属医院 | Material for repairing spinal cord injury and preparation method of bone and spinal cord tissue engineering scaffold |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5116824A (en) * | 1985-05-02 | 1992-05-26 | Katakura Chikkarin Co., Ltd. | Biomaterial comprising a composite material of a chitosan derivative and collagen derivative |
| CN1380115A (en) * | 2002-03-25 | 2002-11-20 | 中国人民解放军第四军医大学 | Preparation process of spinal cord and peripheral nerve repairing material |
| CN1546182A (en) * | 2003-12-15 | 2004-11-17 | 中国人民解放军第四军医大学 | Composite tissue engineering nerve injury repairing material and preparation method thereof |
-
2009
- 2009-01-16 CN CN201210188002.9A patent/CN102671237B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5116824A (en) * | 1985-05-02 | 1992-05-26 | Katakura Chikkarin Co., Ltd. | Biomaterial comprising a composite material of a chitosan derivative and collagen derivative |
| CN1380115A (en) * | 2002-03-25 | 2002-11-20 | 中国人民解放军第四军医大学 | Preparation process of spinal cord and peripheral nerve repairing material |
| CN1546182A (en) * | 2003-12-15 | 2004-11-17 | 中国人民解放军第四军医大学 | Composite tissue engineering nerve injury repairing material and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 刘文静等.应用胶原-壳聚糖桥接管引导大鼠坐骨神经再生.《解剖学杂志》.2008,第1卷(第3期), |
| 应用胶原-壳聚糖桥接管引导大鼠坐骨神经再生;刘文静等;《解剖学杂志》;20081231;第1卷(第3期);全文 * |
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