CN102703302A - Installing seat for automatically clamping sequencing chip - Google Patents

Abstract

The invention discloses an installing seat for automatically clamping a sequencing chip, which comprises an installing cavity for accommodating the sequencing chip. One end of the installing cavity is provided with a positioning ring for positioning the sequencing chip. The installing seat is characterized in that a plurality of positioning grooves running through an inner wall are arranged on the inner wall of the installing cavity along the radial direction of the installing cavity; the positioning grooves are uniformly distributed along the circumference of the installing cavity; and a sliding tongue plate is arranged in each positioning groove, wherein each sliding tongue plate slides along the corresponding positioning groove to extend into the installing cavity to clamp the sequencing chip so as to ensure the sequencing chip to be attached to the positioning ring and retracts into the positioning groove along the positioning groove to loosen the sequencing chip. According to the installing seat for automatically clamping the sequencing chip, which is disclosed by the invention, the sliding tongue plates which can be automatically popped up are set to extend into the installing cavity to clamp the sequencing chip and retract into the corresponding installation positioning grooves under the action of an external force, so that the rapid assembly and disassembly of the sequencing chip are implemented and the sequencing efficiency is improved.

Description

Mounting seat for automatic clamping of sequencing chips

technical field

The invention relates to the technical field of DNA sequencing, in particular to an installation seat for automatically holding a sequencing chip.

Background technique

Pyrosequencing technology is an enzyme cascade chemiluminescent reaction in the same reaction system catalyzed by four enzymes. The principle of pyrosequencing technology is: after the primers anneal to the template DNA, the four enzymes DNA polymerase (DNA polymerase), ATP sulfurylase (ATP sulfurylase), luciferase (luciferase) and adenosine triphosphate (Apyrase) Under the synergistic effect of the primer, the polymerization of each dNTP on the primer is coupled with the release of an optical signal, and the purpose of real-time determination of DNA sequence is achieved by detecting the release and intensity of light. The reaction system of pyrosequencing technology consists of reaction substrates, single strands to be tested, sequencing primers and four enzymes. The reaction substrates are 5'-phosphosulfate (adenosine-5'-phosphosulfate, APS) and luciferin.

In each round of sequencing reaction, only one deoxynucleotide triphosphate (dNTP) is added to the reaction system. If it can just pair with the next base of the DNA template, it will be added under the action of DNA polymerase. To the 3' end of the sequencing primer, a molecule of pyrophosphate (PPi) is released at the same time. Under the action of ATP sulfurylase, the generated PPi can combine with APS to form ATP, and under the catalysis of luciferase, the generated ATP can combine with luciferin to form oxyluciferin, and at the same time generate visible light. A specific detection peak can be obtained through a weak light detection device and processing software, and the height of the peak is proportional to the number of matching bases. If the added dNTP cannot pair with the next base of the DNA template, the above reaction will not occur, and no peak will be detected. The remaining dNTPs and a small amount of residual ATP in the reaction system were degraded under the action of Apyrase. After the previous round of reaction is completed, another dNTP is added to repeat the above reaction, and the accurate DNA sequence information can be read according to the obtained peak diagram.

The overall operation process is described as follows: After the DNA sample is crushed, the library construction reagent is used to add adapters, single-strand capture, bind to microspheres, microemulsion PCR amplification, and emulsion breaking to obtain a DNA library built on microspheres. The template lays the library and the enzymes required for the sequencing reaction on the sequencing chip with a micro-reaction pool, installs the sequencing chip and sequencing reagents on the host computer, and starts the sequencing program by controlling the computer according to the number and position of the modules, and automatically performs the sequencing reaction. The data is transmitted to the data analysis computer, and after the sequencing is completed, the calculation and analysis software is used for image processing, sequence readout, quality analysis, sequence splicing, etc., and finally the sequence information of the DNA sample is obtained.

Before performing the sequencing reaction, it is necessary to install the sequencing chip into the mounting chamber of the mount for the sequencing reaction. Therefore, how to quickly load and unload the sequencing chip becomes an important issue affecting the sequencing efficiency.

Contents of the invention

The problem to be solved by the present invention is to provide a mounting base for conveniently installing the sequencing chip, so as to quickly load and unload the sequencing chip and improve the working efficiency of sequencing.

In order to solve the above problems, the present invention provides a mounting seat for automatically clamping the sequencing chip, including an installation cavity for accommodating the sequencing chip, and one end of the mounting cavity is provided with a positioning ring for positioning the sequencing chip , the inner wall of the installation cavity is provided with a plurality of positioning grooves passing through the inner wall along its radial direction, the positioning grooves are evenly distributed along the circumference of the installation cavity, each of the positioning grooves is provided with a The positioning groove slides into the installation cavity to hold the sequencing chip so that it is close to the positioning ring and retracts into the positioning groove along the positioning groove to release the sliding of the sequencing chip tongue plate.

Preferably, the size of a section of the positioning groove close to the installation cavity is small, forming a first step for limiting the sliding tongue; the end of the positioning groove away from the installation cavity is provided with the The mounting seat is fixedly connected to the baffle plate; one end of the sliding tongue extends into the installation cavity to hold the sequencing chip, and the sliding tongue is provided with a stopper when one end of the sliding tongue is stretched out. The protruding part on the first step; an elastic piece is provided between the protruding part and the baffle to make the sliding tongue extend into the installation cavity.

Preferably, the elastic member is a spring.

Preferably, the spring is sleeved on the sliding tongue between the protrusion and the baffle, and the baffle is provided with a A through hole is provided on the inner side of the baffle plate with a sleeve whose inner diameter is larger than the through hole, and a second step for making the end of the spring lean against is formed between the sleeve and the through hole.

Preferably, the number of the positioning grooves is 2 or 4.

Preferably, the positioning groove is in the shape of a circular hole.

The mounting seat of the present invention that automatically holds the sequencing chip extends into the installation cavity by setting a sliding tongue that can automatically pop up to hold the sequencing chip, and retracts into the installation positioning groove under external force to realize fast loading and unloading of the sequencing chip, improving Sequencing efficiency.

Description of drawings

Fig. 1 is the front view of the installation seat of the automatic clamping sequencing chip of the embodiment of the present invention when the sequencing chip is not installed;

Fig. 2 is the A-A direction sectional view of Fig. 1;

Fig. 3 is a front view of the installation seat of the automatic clamping sequencing chip of the embodiment of the present invention when the sequencing chip is installed;

Fig. 4 is the B-B direction sectional view of Fig. 3;

Fig. 5 is a C-C cross-sectional view of Fig. 4;

Fig. 6 is a schematic front view of the sliding tongue in the mounting base for automatically holding the sequencing chip according to the embodiment of the present invention;

Fig. 7 is a schematic left view of Fig. 6;

Fig. 8 is a three-dimensional schematic diagram of the baffle plate that automatically holds the sequencing chip according to the embodiment of the present invention;

Fig. 9 is a side view of Fig. 8;

Fig. 10 is a sectional view along the line D-D of Fig. 9 .

Explanation of main reference signs

1-Mounting seat 2-Sliding tongue plate 3-Spring

4-Baffle 5-Sequencing chip 6-Positioning groove

11-locating ring 21-raised part 45-set

19-Installation cavity 41-Screw 63-First step

44-through hole 49-second step

Detailed ways

The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but not as a limitation of the present invention.

As shown in Figures 1 to 5, the mounting seat 1 for automatically holding the sequencing chip according to an embodiment of the present invention includes an installation cavity 19 for accommodating the sequencing chip 5, and one end of the mounting cavity 19 is provided with a The positioning ring 11 of 5, the inner wall of the installation cavity 19 is provided with a positioning groove 6 along its radial direction, and the positioning groove 6 is provided with a positioning groove 6 that slides along the positioning groove 6 and extends into the installation cavity 19 to hold the sequencing chip 5 so that it is attached to the positioning ring 11. On the ring 11 or retract into the positioning groove 19 to release the sliding tongue plate 2 of the sequencing chip 5 . The distance between the sliding tongue 2 and the positioning ring 11 is just equal to the thickness of the sequencing chip when the sliding tongue 2 is stretched out, and the sequencing chip 5 can be tightly attached to the positioning ring 11 when the sliding tongue 2 is stretched out. The positioning grooves 6 are symmetrically arranged on the inner wall of the installation cavity 19 and penetrate the inner wall, and the number can be two or four, etc. in an even number. In order to stably clamp the sequencing chip 5, the positioning grooves 6 are along the circumferential direction of the installation cavity 19 Evenly distributed. Of course, more positioning grooves 6 can be provided in order to increase the clamping force, but more positioning grooves 6 will cause inconvenience in operation. In this embodiment, there are two positioning grooves 6, and for the convenience of processing, the positioning grooves 6 are in the shape of round holes.

As shown in Figure 2, the size of a section of the positioning groove 6 close to the installation cavity 19 is relatively small, forming a first step 63 for limiting the sliding tongue plate 2; that is, the section of the positioning groove 6 close to the installation cavity 19 is a smaller round hole, and the rest is a larger round hole. As shown in FIG. 6 and FIG. 7 , the sliding tongue plate 2 is provided with a protruding protrusion 21 . As shown in Figure 4, when the sliding tongue plate 2 extends into the installation cavity 19, the raised portion 21 of the sliding tongue plate 2 abuts against the first step 63, and one end of the sliding tongue plate 2 extends into the installation cavity 19 Inside to hold the sequencing chip 5. The outer end of the positioning groove 6 is provided with a baffle 4 fixedly connected to the mounting seat 1, and a spring 3 is provided between the raised portion 21 of the sliding tongue 2 and the baffle 4. In this embodiment, the spring 3 is sleeved on The other end of the sliding tongue plate 2 and the two ends of the spring 3 abut against the raised portion 21 and the baffle plate 4 respectively.

As shown in FIG. 5 , the baffle plate 4 is fixedly connected with the mounting base 1 by two screws 41 , which is convenient for installation and disassembly. The spring 3 is sleeved on the sliding tongue 2 between the raised portion 21 and the baffle 4, as shown in Figure 8-10, the baffle 4 is provided with a hole for the end of the sliding tongue 2 away from the installation cavity 19 to pass through. A through hole 44 is provided on the inner side of the baffle plate 4 with a sleeve 45 whose inner diameter is larger than that of the through hole 44 , and a second step 49 is formed between the sleeve 45 and the through hole for making the end of the spring 3 lean against it.

In this embodiment, the spring 3 can also be replaced by other elastic parts, and the baffle 4 can also use other parts, as long as it can be against the other end of the spring 3 . When installing the sequencing chip 5, move the end of the sliding tongue 2 protruding into the installation cavity 19 to retract it into the positioning groove 6, and the raised part 21 of the sliding tongue 2 squeezes the spring 3, and the sequencing chip 5 is put into the installation cavity 19 and attached to the positioning ring 11, and then the sliding tongue 2 is released, and the sliding tongue 2 is stretched out under the action of the spring 3, and is clamped on the side of the sequencing chip 5, so that the sequencing chip 5 is tightly attached to the On the positioning ring 11, it is ensured that the sequencing chip 5 will not come out even when it is turned upside down.

Certainly, what is described above is a preferred embodiment of the present invention. It should be pointed out that for those of ordinary skill in the art, some improvements and modifications can also be made without departing from the principles of the present invention. These improvements and modifications It is also regarded as the protection scope of the present invention.

Claims (6)

1. mount pad that holds sequence testing chip automatically; Comprise the installation cavity that is used for ccontaining said sequence testing chip; One end of said installation cavity is provided with the retaining ring that is used to locate said sequence testing chip; It is characterized in that; It radially is provided with the locating slot of the said inwall of a plurality of perforations the inwall upper edge of said installation cavity, and said locating slot is along the circumference uniform distribution of said installation cavity, and being equipped with in each said locating slot slides to stretch in the said installation cavity to hold said sequence testing chip along said locating slot is close on the said retaining ring it and withdraws in the said locating slot to unclamp the slip hyoplastron of said sequence testing chip along said locating slot.

2. the mount pad that holds sequence testing chip automatically as claimed in claim 1 is characterized in that, said locating slot is less near one section size of said installation cavity, is formed for the first step of spacing said slip hyoplastron; Said locating slot is provided with the baffle plate that is fixedly connected with said mount pad away from an end of said installation cavity;

It is interior to hold said sequence testing chip that one end of said slip hyoplastron stretches into said installation cavity, and said slip hyoplastron is provided with and when an end of said slip hyoplastron stretches out, is against the boss on the said first step;

Be provided with between said boss and the said baffle plate and make said slip hyoplastron stretch into the elastic component in the said installation cavity.

3. the mount pad that holds sequence testing chip automatically as claimed in claim 2 is characterized in that, said elastic component is a spring.

4. the mount pad that holds sequence testing chip automatically as claimed in claim 3; It is characterized in that; Said spring housing is on the said slip hyoplastron between said boss and the said baffle plate; Said baffle plate is provided with the through hole that supplies said slip hyoplastron to pass away from an end of said installation cavity, and the inboard of said baffle plate is provided with the cover of internal diameter greater than said through hole, the end that is formed for making said spring between said cover and the through hole against second step.

5. according to claim 1 or claim 2 the mount pad that holds sequence testing chip automatically is characterized in that the quantity of said locating slot is 2 or 4.

6. according to claim 1 or claim 2 the mount pad that holds sequence testing chip automatically is characterized in that said locating slot is a circle hole shape.

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