CN1027772C - Micro uniform quantitative distributor for enzyme labelling liquid by equal liquid level height method - Google Patents
Micro uniform quantitative distributor for enzyme labelling liquid by equal liquid level height method Download PDFInfo
- Publication number
- CN1027772C CN1027772C CN 92115000 CN92115000A CN1027772C CN 1027772 C CN1027772 C CN 1027772C CN 92115000 CN92115000 CN 92115000 CN 92115000 A CN92115000 A CN 92115000A CN 1027772 C CN1027772 C CN 1027772C
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- China
- Prior art keywords
- liquid
- container
- liquid level
- jar
- stop valve
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- 239000007788 liquid Substances 0.000 title claims abstract description 166
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 28
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 28
- 238000002372 labelling Methods 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims abstract description 10
- 238000002347 injection Methods 0.000 claims abstract description 45
- 239000007924 injection Substances 0.000 claims abstract description 45
- 239000012530 fluid Substances 0.000 claims description 21
- 239000002699 waste material Substances 0.000 description 11
- 210000004907 gland Anatomy 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 241000628997 Flos Species 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
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- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A micro-quantity uniform quantitative device of enzyme labeling liquid by an equal liquid level height method belongs to the field of enzyme labeling instruments of medical systems, and simply solves the problem of repeatedly uniform micro-quantity quantitative liquid injection of sample holes of an enzyme labeling disc. The upper layer and the liquid storage tank with the liquid level maintaining function form a communicating vessel by using a double-layer container, liquid injection pipes are fixed on the bottom surface of the lower layer, the two layers are connected by using stop valves, and the outflow of each liquid injection pipe is uniform and stable by ensuring that the free liquid level in the container is stable and the height of the free liquid level in the container from the upper opening of each liquid injection pipe is equal during liquid injection; and the lower layer is filled with liquid and isolated from the upper layer by closing the stop valve to stop outflow, so that micro uniform quantitative distribution of the enzyme labeling liquid is realized.
Description
The present invention relates to a kind of enzyme labelling liquid by isoliquid level height method uniform microquantity dispensing equipment, belong to the technical field of the microplate reader device of medical system.
At present in medical system, cleaning in the work of enzyme linked immunological external test or bag are distributed fluid injection work by the enzyme mark with the required repeatedly micro quantitative determination of the single or multiple or whole plate sample well of dish, normally adopt syringe type apparatus or compression pump that liquid is decided volume ground and are divided in a plurality of sample wells of enzyme mark with dish by conduit.This type of apparatus structure complexity is accurate, and gets over for a long time when the container number that needs fluid injection simultaneously, guarantee that reservoir quantity is evenly also more difficult.
The objective of the invention is to provide a kind of method that waits liquid level, it has solved simultaneously a plurality of enzymes marks better with dish sample well even micro quantitative determination fluid injection problem repeatedly.Another object of the present invention provides a kind of enzyme labelling liquid uniform microquantity dispensing equipment.
The object of the present invention is achieved like this, adopts isoliquid level height method to realize that enzyme mark liquid equal and quantitative distributes.Use the container of a bilayer, upper strata container head opening communicates with atmosphere, a plurality of identical liquid injection pipes are fixed on lower floor container bottom plane, the liquid injection pipe number is corresponding with the sample well number of dish with the enzyme mark, caliber size is selected by following condition: when this liquid injection pipe as upper end closed and the outlet vertically downward of the container of full of liquid, fluid column pressure is during again less than atmospheric pressure, and fluid injection can not go out stream from the mouth of pipe in the container.All pipes suitable for reading is in same plane.Have a controllable stop valve to be connected between the levels of double container, during this opening of valves, the levels container interconnects, and the levels container is isolated mutually when closing.
Upper strata container and the fluid reservoir with maintenance liquid level function are connected into linker, adjusting double container makes it be in horizontal state, open controllable stop valve, in fluid reservoir, inject liquid, make that liquid reaches the liquid level that is guaranteed by fluid reservoir in the double container.Because the liquid level upper end communicates with atmosphere, so the container bottom liquid injection pipe will have liquid outwards to flow out continuously.
In the fluid column under the action of gravity, the each point pressure of same surface level equates that all the suitable for reading of all liquid injection pipes all equates owing to being in the height of same surface level to the upper strata vessel level, thereby each mouth of pipe pressure also equates; In addition since in the container of upper strata liquid level be subjected to the effect of fluid reservoir to keep certain, can be because of fluid injection does not change, so the stream that goes out of each liquid injection pipe all is the identical stable stream that goes out.Close controllable stop valve, go out stream and stop immediately.This is the state that is in full of liquid and isolates fully with the upper strata container owing to lower floor's container, and the pressure that internal tank liquid is annotated is just used stream and stopped less than ambient atmosphere pressure.Thereby the opening time of controllable stop valve can determine the outflow of each liquid injection pipe.
Mark the needs that clean repeatedly with the dish sample well at enzyme, increase a Mead-Bauer recovery system: in lower floor's container, bury a waste collection box underground, its bottom is being fixed and the corresponding waste liquid suction tube of liquid injection pipe, suction tube passes liquid injection pipe, form concentric tubes, the length that liquid notes pipe is stretched out in the suction tube lower end is consistent with the sample well degree of depth of dish with the enzyme mark, and when the suction tube end opening was inserted into the sample well bottom, the liquid injection pipe end opening just in time was in the sample aperture.Suction tube is suitable for reading to exceed waste collection box bottom surface, can prevent the waste liquid sample well that flows backwards back.Can use a vacuum aspirator that the sample well suction is clean during cleaning.When concentric tubes can prevent fluid injection under liquid injection pipe the interruption-forming drop stop phenomenon.
Fig. 1. the synoptic diagram of enzyme labelling liquid uniform microquantity dispensing equipment.
Now in conjunction with the accompanying drawings the structure of enzyme labelling liquid uniform microquantity dispensing equipment is described in detail:
By Fig. 1, equipped the putting by liquid distributor chamber (1) and liquid level chamber (2) of one's duty constitutes double container.Liquid distributor chamber (1) bottom surface is fixed with several identical liquid injection pipes (3), and the liquid injection pipe number uses the sample well number of dish corresponding with the enzyme mark [expression is 3 among the figure], and each liquid injection pipe (3) is suitable for reading all at grade; A waste collection box (4) is housed in the liquid distributor chamber (1), and box (4) bottom surface also is fixed with and the suction tube (5) of liquid injection pipe (3) with quantity.Suction tube (5) is inserted into and forms concentric tubes in the liquid injection pipe (3), and stretch out its upper end, and stretch in the waste collection box (4); Also stretch out the lower end, and extension length and enzyme mark is consistent and enter the enzyme mark and use and coil in the sample well (6) with the dish sample well degree of depth; The side bottom of waste collection box (4) also is provided with the waste liquid vent pipe (7) that an effluent discharge is used.Liquid distributor chamber (1) top by stop valve (8) communicating pipe (9) top with liquid level chamber (2) be connected; Be higher than in the liquid distributor chamber (1) that waste collection box (4) is located and connect a controllable stop valve (10) and communicate with liquid level chamber (2) bottom again by connecting pipe.
The liquid storage system mainly is socketed to form by jar (16) in liquid storage outer jar (15) and the liquid storage.It has the function that keeps liquid level, and the cavity between interior jar (16) and outer jar (15) is liquid level holding chamber (14), and the top of jar (16) is not connected in liquid level holding chamber (14) and the liquid storage.The top of jar (16) is provided with the liquid injection port (23) of with closure (20) in the liquid storage.The top of liquid level holding chamber (14) also has the top of an outlet (24) by connecting pipe (11) and liquid level chamber (2) and communicates, and communicates with atmosphere by a stop valve (21) again.Interior jar (a 16) bottom centre position has an outlet to connect a stop valve (19) as the fluid reservoir floss hole.Liquid level holding chamber (14) bottom has an outlet to connect a stop valve (13) in addition and joins with the middle part of liquid level chamber (2).Liquid level maintenance face M2(22 on the sidewall of liquid storage interior jar (16)) locates to have liquid level hole (17).
The size in liquid level hole (17) should satisfy following condition: it is airtight and fill with the container of liquid that jar (16) become upper end in liquid storage, when liquid level hole (17) was exposed in the atmosphere again, the size in hole must make atmosphere can break through the interfacial film of aperture place's liquid and atmosphere and enter jar (16) in the liquid storage.The quantity in hole selects for the total flow of the each fluid injection of sample well (6) by liquid injection pipe (3).The sidewall of jar (16) is lower than liquid level and keeps face M2(22 in the liquid storage in addition) the place many connection bottom outlets (18) are arranged, jar (16) is communicated with each other.
This liquid equal and quantitative distributor operating principle is as follows: at first the levelling of the baseplane of liquid distributor chamber (1) is horizontality when using, close stop valve (13) (19), open stop valve (21), open jar (16) gland bonnet (20) in the liquid storage and in liquid storage, fill with liquid in jar (16), then gland bonnet (20) is sealed, close controllable stop valve (10) again, open stop valve (13), make liquid level chamber (2) and liquid level holding chamber (14) form a linker that communicates with atmosphere, what is that liquid level reaches unanimity, and the liquid in the liquid storage in jar (16) is also because the airtight and unlikely outflow in upper end.
When the liquid level in the liquid level holding chamber (14) drops to liquid level hole (17) when following, gas is clamp-oned jar (16) in the liquid storage in the atmosphere, and make the liquid storage pot liquid flow into liquid level holding chamber (14) through being communicated with bottom outlet (18), and process stop valve (13) flows into liquid level chamber (2) again, liquid level M1(12 in liquid level chamber (2)) with liquid level holding chamber (14) in liquid level keep face M2(22) become when consistent, liquid is static with regard to reaching balance.Close stop valve (21) this moment, and can close stop valve (13) as required again and repeat jar (16) fluid injection in liquid storage, and fluid injection finishes and gland bonnet (20) sealed again.Open stop valve (8) and controllable stop valve (10), liquid is influent distributor chamber (1) immediately, and liquid injection pipe (3) will have liquid to flow out.But after the liquid that injects plane rapidly that liquid injection pipe (3) is suitable for reading covered, liquid distributor chamber (1) liquid level chamber (2) liquid level holding chamber (14) was formed a linker jointly, and and liquid storage in jar (16) together with atmospheric isolation.Interior pressure in the whole linker and atmospheric pressure be balance gradually, makes liquid injection pipe (3) go out stream and stops.But the liquid level in the linker tends to balance and does not stop, gas in the liquid distributor chamber (1) flows to liquid level chamber (2) through stop valve (8), pass through communicating pipe (11) again and flow to liquid level holding chamber (14), the interior liquid level of liquid level holding chamber (14) descends, liquid flows into liquid level chamber (2) through stop valve (13) and passes through controllable stop valve (10) influent distributor chamber (1) again, fill with liquid distributor chamber (1) at last, the interior liquid level M1(12 in liquid level chamber (2)) reach liquid level and keep face M2(22), liquid reaches balance again and static in the entire container.If close stop valve (8) and controllable stop valve (10), open stop valve (21) and make liquid level M1(12), M2(22) all be under the atmospheric pressure effect; When open controllable stop valve (10), just have stablize uniformly the flow liquid body flows into from liquid injection pipe (3) end opening along suction tube (5) outer wall the enzyme mark with the sample well (6) that coils; Otherwise if close stop valve (10), fluid injection just stops immediately; The enzyme mark can be taken away by waste liquid vent pipe (7) by a vacuum aspirator with the waste liquid in the dish sample well (6).If use a program timer, the open and close program and the time of control controllable stop valve (10) and vacuum aspirator, just can realize rationed uniformly to the enzyme labelling liquid trace.
Compared with prior art, the uniform microquantity dispensing equipment of enzyme labelling liquid by isoliquid level height method of the present invention:
1. the employing isoliquid level height method makes distributor simple in structure, can the uniform distribution fluid injection, and need not to be subjected to the container number of simultaneously fluid injection to limit.
2. can adopt the time-program(me) control mode that is easy to realize, control outflow by the control time.
Claims (3)
1, a kind of enzyme labelling liquid by isoliquid level height method uniform microquantity dispensing equipment, it is characterized in that using a double container, lower floor's container bottoms is fixed with a plurality of identical liquid injection pipes, all pipes are suitable for reading to be in same plane, caliber size is by following condition selection: when this liquid injection pipe as upper end closed and be full of the outlet vertically downward of the container of enzyme labelling liquid, fluid column pressure is during again less than atmospheric pressure, enzyme labelling liquid in the container can not flow out from the mouth of pipe, be connected with a controllable stop valve between the levels of double container, the levels container is interconnected during this opening of valves, the levels container is isolated mutually when closing, the upper strata container is connected with a liquid storage system again, the liquid storage system mainly is socketed to form by in outer jar of liquid storage and the liquid storage jar, cavity between interior jar and outer jar is the liquid level holding chamber, the top of jar is not connected in liquid level holding chamber and the liquid storage, the top of jar is provided with the liquid injection port of with closure in the liquid storage, the top of liquid level holding chamber has an outlet and connects mutually with the top of upper strata container and communicate with atmosphere through a stop valve, and the bottom has one to export and the middle part of upper strata container joins in addition.
2, distributor according to claim 1 has on the sidewall that it is characterized in that in the liquid storage jar and makes the hole that jar is connected with the bottom of liquid level holding chamber in the liquid storage.
3, easy to use it is characterized in that of installing according to claim 1:
1). adjust double container, make it be in horizontal state, and the upper strata of double container is communicated with atmosphere;
2). keep the fluid reservoir of liquid level function in double container, to inject liquid by having, container interior liquid level in upper strata is remained unchanged;
3). open the controllable stop valve that is communicated with the double container levels, make the container bottom liquid injection pipe have liquid outwards to flow out continuously;
4). close above-mentioned controllable stop valve, liquid injection pipe liquid flows out and stops;
5). control the outflow of the opening time of above-mentioned controllable stop valve with the decision liquid injection pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92115000 CN1027772C (en) | 1992-12-29 | 1992-12-29 | Micro uniform quantitative distributor for enzyme labelling liquid by equal liquid level height method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92115000 CN1027772C (en) | 1992-12-29 | 1992-12-29 | Micro uniform quantitative distributor for enzyme labelling liquid by equal liquid level height method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1072263A CN1072263A (en) | 1993-05-19 |
| CN1027772C true CN1027772C (en) | 1995-03-01 |
Family
ID=4947218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92115000 Expired - Fee Related CN1027772C (en) | 1992-12-29 | 1992-12-29 | Micro uniform quantitative distributor for enzyme labelling liquid by equal liquid level height method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1027772C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101074948B (en) * | 2006-05-17 | 2011-09-07 | 深圳迈瑞生物医疗电子股份有限公司 | Apparatus and method for collecting and dispensing sampler by single liquid-feeding tube |
| CN101074949B (en) * | 2006-05-17 | 2011-07-13 | 深圳迈瑞生物医疗电子股份有限公司 | Apparatus and method for dispensing and washing sampler |
| CN102060124B (en) * | 2010-11-05 | 2012-11-28 | 陈银环 | Liquid storage device and automatic liquid supply device |
| CN116482397B (en) * | 2023-05-04 | 2025-08-19 | 重庆钢铁股份有限公司 | Quantitative liquid inlet device suitable for online chemical analysis of steam |
-
1992
- 1992-12-29 CN CN 92115000 patent/CN1027772C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1072263A (en) | 1993-05-19 |
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| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |