CN102796187B - 基于RNAi技术防治害虫的新方法 - Google Patents

基于RNAi技术防治害虫的新方法 Download PDF

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Publication number
CN102796187B
CN102796187B CN201210167972.0A CN201210167972A CN102796187B CN 102796187 B CN102796187 B CN 102796187B CN 201210167972 A CN201210167972 A CN 201210167972A CN 102796187 B CN102796187 B CN 102796187B
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seq
nucleic acid
sequence
polynucleotide
insects
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CN102796187A (zh
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苗雪霞
张�浩
李海超
王玉冰
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Center for Excellence in Molecular Plant Sciences of CAS
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Shanghai Institutes for Biological Sciences SIBS of CAS
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    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46N-acyl derivatives
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60Isolated nucleic acids
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/14Type of nucleic acid interfering nucleic acids [NA]
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    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • General Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Insects & Arthropods (AREA)
  • Microbiology (AREA)
  • Agronomy & Crop Science (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Virology (AREA)
CN201210167972.0A 2011-05-27 2012-05-25 基于RNAi技术防治害虫的新方法 Expired - Fee Related CN102796187B (zh)

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CN201210167972.0A CN102796187B (zh) 2011-05-27 2012-05-25 基于RNAi技术防治害虫的新方法

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CN201110142100 2011-05-27
CN201110142100.4 2011-05-27
CN201210167972.0A CN102796187B (zh) 2011-05-27 2012-05-25 基于RNAi技术防治害虫的新方法

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CN102796187B true CN102796187B (zh) 2014-07-16

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WO (1) WO2012163084A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103053480B (zh) * 2013-01-25 2014-04-16 中国农业科学院柑桔研究所 一种柑橘全爪螨若螨的rna干扰方法
BR112017007084A8 (pt) * 2014-10-13 2023-02-07 Dow Agrosciences Llc Moléculas de ácido nucleico subunidade copi coatomer alfa que confere resistância a pragas de coleóptero e hemípteros
CN107858405B (zh) * 2017-10-12 2021-09-24 华南农业大学 一种测定外源dsRNA对瓢虫毒性影响的方法
CN108949770B (zh) * 2018-07-24 2021-11-16 江西农业大学 棉铃虫e75基因及其在rna介导的害虫防治应用
CN109077028B (zh) * 2018-08-28 2023-07-18 中国农业科学院蔬菜花卉研究所 对西花蓟马进行rna干扰的方法及装置
CN109169534A (zh) * 2018-10-15 2019-01-11 南开大学 线虫固体培养基及其制备方法和应用
CN112831506B (zh) * 2019-11-22 2023-08-15 深圳大学 一种黄曲条跳甲细胞色素p450基因及其应用
CN111944824B (zh) * 2020-08-24 2022-11-08 东北林业大学 美国白蛾速激肽受体基因及dsRNA和防治美国白蛾中应用
CN113430200B (zh) * 2021-08-17 2022-05-27 中国林业科学研究院森林生态环境与保护研究所 美国白蛾vATPase A基因dsRNA及其细菌表达液及应用
CN118207217B (zh) * 2024-01-22 2025-04-15 东北林业大学 一种美国白蛾核酸农药微胶囊剂及其应用
CN118303422B (zh) * 2024-04-17 2024-11-12 华南师范大学 Ppk基因在控制德国小蠊繁殖行为中的应用
CN119570821A (zh) * 2024-11-23 2025-03-07 安徽省林业科学研究院 松褐天牛三磷酸腺苷酶基因V-ATPase H及其应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
PsOr1, a potential target for RNA interference-based pest management;Y. Y. Zhao等;《Insect Molecular Biology》;20100921;第20卷(第1期);97-104 *
RNA干扰技术在植物线虫基因功能研究及虫害防治方面的应用;王高峰 等;《生物技术通报》;20081231(第5期);75-80 *
Second-Generation Sequencing Supply an Effective Way to Screen RNAi Targets in Large Scale for Potential Application in Pest Insect Control;Yubing Wang等;《PLoS ONE》;20110430;第6卷(第4期);e18644 *

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WO2012163084A1 (fr) 2012-12-06

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