CN1030902A - Remove the biological purification method of methyl alcohol in the sewage - Google Patents
Remove the biological purification method of methyl alcohol in the sewage Download PDFInfo
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- CN1030902A CN1030902A CN87105219.9A CN87105219A CN1030902A CN 1030902 A CN1030902 A CN 1030902A CN 87105219 A CN87105219 A CN 87105219A CN 1030902 A CN1030902 A CN 1030902A
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- CN
- China
- Prior art keywords
- sewage
- methyl alcohol
- bacterial strain
- phosphoric acid
- industrial
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 title claims abstract description 211
- 239000010865 sewage Substances 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims abstract description 41
- 238000000746 purification Methods 0.000 title claims description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims abstract description 28
- 230000001580 bacterial effect Effects 0.000 claims abstract description 24
- 241000589344 Methylomonas Species 0.000 claims abstract description 16
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims abstract description 14
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims abstract description 5
- 238000009395 breeding Methods 0.000 claims abstract description 4
- 230000001488 breeding effect Effects 0.000 claims abstract description 4
- 238000005352 clarification Methods 0.000 claims abstract description 3
- 230000000813 microbial effect Effects 0.000 claims abstract description 3
- 238000011160 research Methods 0.000 claims abstract description 3
- 244000005700 microbiome Species 0.000 claims description 21
- 239000002351 wastewater Substances 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 2
- 230000003472 neutralizing effect Effects 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 5
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 abstract description 4
- 229910000037 hydrogen sulfide Inorganic materials 0.000 abstract description 4
- 238000004321 preservation Methods 0.000 abstract description 4
- 239000007789 gas Substances 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 16
- 235000007164 Oryza sativa Nutrition 0.000 description 16
- 235000009566 rice Nutrition 0.000 description 16
- 230000002000 scavenging effect Effects 0.000 description 9
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 239000003345 natural gas Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000320412 Ogataea angusta Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000027288 circadian rhythm Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 235000019156 vitamin B Nutrition 0.000 description 2
- 239000011720 vitamin B Substances 0.000 description 2
- AUTOLBMXDDTRRT-JGVFFNPUSA-N (4R,5S)-dethiobiotin Chemical compound C[C@@H]1NC(=O)N[C@@H]1CCCCCC(O)=O AUTOLBMXDDTRRT-JGVFFNPUSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004081 cilia Anatomy 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Invention belongs to the method for utilizing the biopurification principle to remove methyl alcohol in the decontaminated water.
The sewage that contains methyl alcohol neutralizes with phosphoric acid earlier, makes it to contain abundant nitrogenous source again, introduces bacterial strain Methylomonas metanicaB-2576 then.Preservation bacterium dwarf's unit is the Research for Industrial Microbial Germ preservation center of genetics and industrial micro breeding Science Institute of reviving entirely.Sewage is being carried out purifying treatment with before removing wherein methyl alcohol, above-mentioned bacterial strains must be placed on the specified substratum and cultivate.
The biological clarification of removing methyl alcohol in the sewage can be used to be engaged in the sky and adopt and process the hydrogen sulfide containing business enterprice sector of opening right gas.
Description
The application's proposed invention belongs to the biological Treatment of Wastewater method, specifically, is to utilize the biopurification principle to remove a kind of method of methyl alcohol in the sewage.
The present invention can be used for some production department that is engaged in exploitation and the hydrogen sulfide containing Sweet natural gas of processing treatment.
At present, knownly can contain the cultivation of carrying out on the cultivation basis of methyl alcohol, here, the effect of methyl alcohol provides carbon, i.e. carbon source.
For example, it is to make various yeast growth (" the Φ и э и о л о г и я и М e т а б о л и э м м т а н о л у с в а и в а г о щ и х д р о ж ж e и " of В С П о д г о р с к ц й work (zymic physiological action and metabolism of digestion methyl alcohol) on the substratum of methyl alcohol containing that a kind of method is arranged, Soviet Union Kiev press " н а у к о в а д у м к а ", 1982).In addition, the yeast method that also has a kind of cultivation can digest methyl alcohol is also carried out on substratum, contains the contamination nutrient solution (claiming " little wine ") that produces through hydrolysis-fermentation in the composition of substratum.（“Acta microbiol.bulg.”，1970，V·7，P49-54）。
Above-mentioned all cultivations can digest the method for microorganism of methyl alcohol, may be used to purify the industrial sewage that contains methyl alcohol.For example, the yeast that a kind of Hansenula Pelymorpha arranged is cultivated containing on the sewage of methyl alcohol, and sewage then is in exploitation and handles in the process of hydrogen sulfide containing Sweet natural gas and produce.So just for purifying this class sewage, the methyl alcohol of removing wherein provides possibility (russian patent SU, A, 963960) to this method.
According to this method, the scavenging process that contains the sewage of methyl alcohol is to utilize yeast under 36-38 ℃ temperature, and in the certain culture base, finish.Requirement for substratum: with the substratum pH3.9-4.0 that neutralizes, wherein should contain abundant trace element simultaneously, for nitrogenous source (claiming nitrogenous source again) and multivitamin with phosphoric acid.Utilize this on record method to go to purify waste water, can only be contained the water of trace carbinol.
The shortcoming of this method is to need to consume a large amount of strong phosphoric acid, and a considerable amount of trace element and multivitamin.Because these all are that to constitute the substratum that is beneficial to this culture growth reproduction that can digest methyl alcohol of Hansenula Polymorpha necessary.For example, if handle 2000 cubic metres sewage, this step of neutralizing effect will need 3 cubic metres strong phosphoric acid, also will consume trace element simultaneously more than 250 kilograms, and 3.6 kilograms VitB1 (vitamins B
1) and 40 desthiobiotins that restrain.The shortcoming of this method also has: dilution rate is low, promptly 0.5 liter/hour; 5 liters=0.1 hour
-1, so just require treating plant must have sizable capacity, because the time that the water that is cleaned stops in equipment is for 10 hours.
Basic task of the present invention is to set up a kind of biological purification method that can remove methyl alcohol in the sewage.In this method, preferably can utilize a kind of microorganism, by its effect, make the scavenging process of sewage more favourable economically on the one hand, the aspect can make process intensification again in addition.
The way that solves this task is as follows: removing the biopurification of methyl alcohol in the sewage, is before the methyl alcohol in removing sewage, culturing micro-organisms on sewage earlier, and must with phosphoric acid sewage being neutralized in advance, and make and contain abundant carbon source in the water.According to the invention of being applied for, the microorganism of employing is bacterial strain Methylomonas metanica B-2576.The unit of preservation strain is: the industrial microorganism bacterial strain preservation center of revive full genetics and industrial micro breeding Science Institute (nineteen eighty-two preservation on July 4).
Because adopt the present invention, the spent chemical reagent of 1 cubic metre of sewage of every purification only is equivalent to 1/12 of the required amount of reagent of present known purifying method, and the intensity of scavenging process improves 1 times.
According to the invention of being applied for, preferably in advance with phosphoric acid with the sewage pH5.5-7.0 that neutralizes, and the cultivation of mentioned microorganism is to carry out under temperature is 20-37 ℃ condition.
Below we by the sewage purification method is described in detail itself, and introduce and implement several specific exampless of the present invention, will further understanding be arranged to objects and advantages of the present invention.
For realizing method proposed by the invention, must separate the microorganism that can digest methyl alcohol.For this reason, the soil that has stain small amount of methanol is put into vertical glass cylinder, order is passed through glass cylinder from the sewage that contains methyl alcohol of Sweet natural gas collection field, and the concentration of methyl alcohol should be diluted to 1 grams per liter with substratum in advance in the sewage.The composition of the inorganic medium that uses is following, and (unit is Grams Per Minute rice
3): K
2HPO
4-1.0; MgSO
47H
2O-0.5; NaCl-0.2; FeSO
47H
2O-0.03; NH
4NO
3-0.5; PH-7.0.Utilize substratum to regulate, the dilute strength of actual sewage is progressively descended, thereby improve the concentration of methyl alcohol in the substratum gradually, reach 10.0 Grams Per Minute rice at last
3The increase of methanol concentration in the sewage is to take place under the situation about being fully utilized of the methyl alcohol in the sewage that formerly dilutes.(wherein containing methyl alcohol is 10.0 Grams Per Minute rice feeding sewage
3) advance cylinder and subsequently in glass cylinder effusive sewage do not contained any methyl alcohol this stage, (composition of substratum as mentioned above, wherein the content of methyl alcohol is 2.0 Grams Per Minute rice because the inclusion of glass cylinder has been inoculated on the inorganic medium of fine jade materialization
3So the pure culture * of bacterium has just separated.The desired methanol concentration of culture digestion methyl alcohol is lower under quiescent conditions, and is in when growing under the continuous mobile condition when culture, and its digests methyl alcohol and then must carry out under the methanol concentration condition with higher.Microorganism can contain methyl alcohol 2.0 Grams Per Minute rice
3Substratum in grow, this * common name " pure culture "-annotation of translation
Prove that fully methyl alcohol can be utilized by microorganism under such condition, because at this moment methyl alcohol is unique carbon source.
Bacterial strain Methylomonas metanica B-2576 aspect biomorph and the feature of Physiology and biochemistry aspect as follows: cell is thin direct rod shape, movability, has the polarity cilium, cell is a Gram-negative, and be aerobic, cell is bred by division, and the size of cell is 0.6 * 1 millimeter; Bacterium colony is pink colour or cream color, and there is mucous membrane on the surface, and the size of bacterium colony is the 3-4 millimeter.
Above-mentioned this bacterial strain can not be grown in the cultivation of general meat peptone agar type, and can only grow containing on the substratum of methyl alcohol.
The industrial culture Methylomonas metanica B-2576 that separates is deposited in the Research for Industrial Microbial Germ preservation center (nineteen eighty-two * preservation on July 4) of full Soviet Union genetics and industrial micro breeding Science Institute.
Remove the theing contents are as follows of biological clarification of methyl alcohol in the sewage that the application proposes: the sewage that will contain methyl alcohol is 5.5-7.0 with the phosphoric acid pH that neutralizes, and makes it contain abundant nitrogenous source again, for example adds ammonium nitrate in sewage.
So putting into bacterial strain Methylomonas metanica B-2576 again in sewage, and this microorganism is cultivated, temperature should keep 20-37 ℃.Studies show that, under 20-37 ℃ condition, cultivate this microorganism, can guarantee that methyl alcohol is fully utilized by bacterium, reach in sewage the only methyl alcohol of residual trace at last, even can reach the stage that does not contain methyl alcohol fully.When temperature was lower than 20 ℃, methyl alcohol can be slowed down greatly by the process that microorganism utilizes, so that in 48 hours of bacterial growth, had only 450 milligrams by the used up methyl alcohol of bacterium in every liter of sewage.When temperature was higher than 37 ℃, bacterial strain Methylomonas metanica B-2576 did not grow, thereby just can not utilize methyl alcohol yet.
Use in advance in the phosphoric acid and during sewage, the pH value must not be lower than 5.5, because when the pH value was low, the speed of growth of we employed this bacterial strain Methylomonas metanica B-2576 can be slack-off, so methyl alcohol is also descended by the efficient that bacterial strain utilizes thereupon., if sewage is weakly alkaline (being that the pH value is greater than 7.0), it also is disadvantageous utilizing methyl alcohol fully for this microorganism so.
The biological Treatment of Wastewater method that the application proposed can be used for purifying and contains high concentration methanol, and promptly containing methyl alcohol is the 5%(quality) sewage.In this case, the methyl alcohol in the sewage can be utilized by microorganism all, and can accomplish not contain fully methyl alcohol in the water after purification, perhaps only contains the methyl alcohol of trace.
Owing to use bacterial strain Methylomonas metanica B-2576 replaced before being used for the purifying waste water another kind of yeast culture Hansenula Polymorpha of methyl alcohol, thereby the scavenging process that makes sewage is economically also than more favourable in the past, this be because, first, in and the spent phosphoric acid consumption of sewage significantly reduced, the method in past requires the sewage pH3.9-4.0 that neutralizes, and present method is only required the pH5.5-7.0 that neutralizes; The second, the growth of bacterial strain Methylomonas metanica B-2576 and the utilization of methyl alcohol all need VitB1 (vitamins B anything but
1) and the participating in of VITAMIN such as desthiobiotin.
Compare with purifying method in the past, owing to reduced the time that sewage retains in treating plant, thereby make the intensity of scavenging process improve 1 times.
When the method for using the application's case to propose goes to carry out the purification of sewage, can accumulate the bacterial biomass that contains 70% above crude protein, this part protein then can be further used for preparing the multiple proteins goods.
Example 1:
To be 50.0 Grams Per Minute rice exploiting and processing the methanol content that obtains in the process of hydrogen sulfide containing Sweet natural gas
3Sewage to inject volume be 5 liters fermentor tank (a kind of fermentor tank that is used for the cultured continuously microorganism).Add phosphoric acid then in fermentor tank, quantity is by 0.4 ml/min rice
3, also must add ammonium nitrate simultaneously, quantity is by 150 milligrams/decimeter
3Calculate.
At first use artificial synthetic substratum with the concentration dilution of methyl alcohol in the sewage to 10 Grams Per Minute rice
3Simultaneously bacterial strain Methylomonas metanica is inoculated in the fermentor tank.Bacterial strain is to cultivate to get up under the condition of rocking slightly on the artificial synthetic medium in the erlenmeyer flask, and the concentration of methyl alcohol is 5.0 Grams Per Minute rice in the substratum
3
By reducing the dilute strength of artificial medium, progressively improving the concentration of methyl alcohol in the sewage, and reach 50.0 Grams Per Minute rice to sewage
3The concentration of phosphor in sewage acid should remain on 0.4 ml/min rice
3(being equivalent to pH6.5).The positive 30 ℃ of concentration when Methylomonas metanica B-2576 bacterial strain of the temperature maintenance of sewage reach 35 ± 5 Grams Per Minute rice
3Afterwards, bacterial body is 0.2 hour at dilution factor just
-1The condition that feeds of effluent stream under the growth procreation, the time that the sewage that the time of this process is equivalent to be cleaned stops in fermentor tank is successively for 5 hours.
Bacterial strain Methylomonas metanica B-2576 is containing on the sewage of methyl alcohol through 10 round the clock continuous growth, and the biomass all-the-time stable of microorganism is at 37 ± 2 Grams Per Minute rice
3Level.The degree that this proof methyl alcohol is utilized by microorganism is very high.Therefore, the sewage after the purification only contains trace carbinol.
Example 2:
It is similar to contain the way of being introduced in the scavenging process of sewage of methyl alcohol and the above-mentioned example 1.But, with this step of sewage, should make the pH value reach 5.5 in going with phosphoric acid in advance.Simultaneously, the temperature of sewage must remain on 20 ℃.
The cultivation of bacterial strain Methylomonas metanica B-2576 is undertaken by circadian rhythm, and the best initial concentration of incubation period methyl alcohol is 1 Grams Per Minute rice
3
Scavenging process must carry out under fierce stirring 48 hours.
Mentioned microorganism is through after 48 hours the cultivation, and the content of methyl alcohol only has 0.002 Grams Per Minute rice in the sewage that is cleaned
3
Example 3:
The way of the scavenging process of sewage and above-mentioned example 1 is similar.But with this step of sewage, the pH value should reach 7.0 in going with phosphoric acid in advance, and the temperature of sewage should remain on 37 ℃ simultaneously.
The cultivation of bacterial strain Methylomonas metanica B-2576 is undertaken by circadian rhythm, and the initial concentration of methyl alcohol is 1 Grams Per Minute rice
3
Scavenging process must carry out under fierce stirring 48 hours.Mentioned microorganism has not contained any methyl alcohol through after 48 hours the cultivation in the sewage that is cleaned.
Claims (3)
1, biological clarification utilization culturing micro-organisms on sewage of removing methyl alcohol in the sewage is carried out.Purify waste water remove methyl alcohol before, must with phosphoric acid sewage be neutralized in advance, also must make simultaneously and wherein contain abundant nitrogenous source, the characteristics of this method are to utilize the microorganism of bacterial strain Methylo-monas metanicaB-2576 as sewage purification process, and the depositary institution of this bacterial strain is the Research for Industrial Microbial Germ preservation center of genetics and industrial micro breeding Science Institute of reviving entirely.
2, the characteristics according to example 1 described method are: sewage must give and neutralizing with phosphoric acid earlier, makes its pH value reach 5.5-7.0.
3, the characteristics of example 1,2 methods that provided are: the culture temperature of bacterial strain Methylomonas metanicaB-2576 is 20-37 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN87105219.9A CN1015889B (en) | 1983-05-06 | 1987-07-30 | Remove the biological purification method of methyl alcohol in the sewage |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU833589002A SU1150892A1 (en) | 1983-05-06 | 1983-05-06 | Method of biochemical purification of waste water of methanol |
| GB8716573A GB2207127B (en) | 1983-05-06 | 1987-07-14 | Process for biological purification of waste waters containing methanol |
| CN87105219.9A CN1015889B (en) | 1983-05-06 | 1987-07-30 | Remove the biological purification method of methyl alcohol in the sewage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1030902A true CN1030902A (en) | 1989-02-08 |
| CN1015889B CN1015889B (en) | 1992-03-18 |
Family
ID=27178875
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN87105219.9A Expired CN1015889B (en) | 1983-05-06 | 1987-07-30 | Remove the biological purification method of methyl alcohol in the sewage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1015889B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1102130C (en) * | 1996-12-21 | 2003-02-26 | 韩国建设技术研究院 | System and method for removing biologically both nitrogen and phosphorous removal in sewage and wastewater |
-
1987
- 1987-07-30 CN CN87105219.9A patent/CN1015889B/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1102130C (en) * | 1996-12-21 | 2003-02-26 | 韩国建设技术研究院 | System and method for removing biologically both nitrogen and phosphorous removal in sewage and wastewater |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1015889B (en) | 1992-03-18 |
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