CN103190889B - Real-time tunable confocal microscopic imaging system - Google Patents

Abstract

The invention relates to a real-time tunable confocal microscopic imaging system, which is characterized in that: the laser emitted by the first laser is reflected by a multi-faceted emitting mirror and then received by a photodiode, and the photodiode sends a sinusoidal signal to an amplification and modulation circuit for processing to generate line synchronization The signal is sent to the programmable logic device, the programmable logic device sends the line synchronization signal to the D/A converter, and the D/A converter converts it into a current signal, and sends it to the galvanometer vibrating mirror drive circuit for modulation and conversion to drive The galvanometer oscillating mirror, the vertical synchronization signal generation module of the programmable logic device counts the horizontal synchronization signal to generate the frame synchronization signal, and the programmable logic device sends the horizontal synchronization signal, the frame synchronization signal and the pixel clock signal to the acquisition card respectively, The acquisition card collects the gray value signal of the sample in the photon counter point by point according to the line synchronization signal, frame synchronization signal and pixel sampling clock signal, and sends the collected image to the computer to reconstruct the real-time video image point by point. The invention can be widely used in the imaging control of the laser scanning microscopic imaging system.

Description

A real-time tunable confocal microscopy imaging system

technical field

The invention relates to an optical imaging system, in particular to a real-time tunable confocal microscopic imaging system suitable for in-body detection.

Background technique

Compared with traditional microscopes, confocal microscopes have the characteristics of high resolution, especially longitudinal high resolution. It can perform optical tomography on the axial direction of the sample, and can reconstruct the three-dimensional image of the sample. In addition, the confocal microscope also breaks through the limitation of the diffraction limit of ordinary optical microscopes. The lateral resolution is 1.4 times that of ordinary optical microscopes with the same numerical aperture, and the longitudinal resolution can reach sub-micron level, so thick biological samples can be axially chromatography. The aperture diaphragm added in front of the detector and the sample makes the scattered light received by the detector only when the sample is in the focal plane, which greatly weakens the influence of stray light, so the system has a high signal-to-noise ratio and the image has Very high contrast and clarity. The biggest advantage of confocal microscopy is that it provides a high-resolution, dye-free, non-invasive imaging method for live detection, and can image within a few hundred micrometers in thick transparent or translucent substances, so it is increasingly Widely used in the field of skin medical imaging.

However, due to the slow imaging speed of traditional confocal imaging (mostly at the second level per frame), its application is greatly limited. At present, confocal microscopy realizes real-time confocal imaging at video rates mainly in the following ways: 1. The two-dimensional scanning imaging method using a resonant mirror plus a galvanometer galvanometer, but using a resonant mirror as a fast-axis scan is sinusoidal rather than linear, so The image obtained in the later period needs to be corrected by the algorithm; 2. The scanning method of the double galvanometer, although this method avoids the nonlinearity of the fast axis scanning, the scanning rate is very slow, even if the high-performance galvanometer is used at a resolution of 512 Under the condition of ×512, it can only reach 3fps; 3. Use polygon mirror as the fast axis galvanometer as the slow axis scanning method, this method is linear scanning, and it does not need to perform nonlinear correction on the image while obtaining a high scanning rate. However, the existing confocal based on polygon mirrors and galvanometers is controlled by some fixed control circuits, the imaging rate cannot be adjusted, and the signal is fixed to a standard signal (such as TV RS-170 interlaced video signal). There are certain restrictions on image acquisition resolution and post-processing.

Contents of the invention

In view of the above problems, the object of the present invention is to provide a real-time tunable confocal microscopic imaging system, which has good versatility and can not only realize confocal scanning imaging with adjustable frame rate and image resolution, but also A multi-choice mode is provided for the user.

To achieve the above object, the present invention adopts the following technical solutions: a real-time tunable confocal microscopic imaging system, characterized in that it includes a laser scanning microscopic imaging system, a first laser, a photodiode and a confocal microscopic imaging control system, the confocal microscopic imaging control system includes a programmable logic device, an amplification modulation circuit, a D/A converter and a galvanometer vibrating mirror drive circuit, and the programmable logic device includes a counting frequency division module and a vertical synchronization signal A generation module, the input end of the counting frequency division module is connected to a crystal oscillator, which is used to generate a pixel sampling clock signal after the counting frequency division of the crystal oscillator signal, and the counting frequency division module sends the pixel sampling clock signal to the laser scanning microscope imaging system The polygon mirror is used to control its rotation; the laser light emitted by the first laser is reflected by the polygon mirror and then received by the photodiode, and as the polygon mirror rotates, the photodiode outputs a sinusoidal voltage signal , and send it to the amplification modulation circuit for processing to generate a line synchronization signal, and send it to the programmable logic device, and the programmable logic device sends the line synchronization signal to the D/A converter, The D/A converter converts the horizontal synchronization signal into a current signal, and sends it to the galvanometer oscillating mirror driving circuit to generate a sawtooth voltage signal, and sends it to the galvanometer oscillating mirror driving control board Drive the galvanometer oscillating mirror of the laser scanning microscopic imaging system to work; the vertical synchronization signal generation module of the programmable logic device counts the horizontal synchronization signal according to actual needs to generate the required frame synchronization signal, and the programmable logic The device sends the line synchronization signal, frame synchronization signal and pixel clock signal to the acquisition card of the laser scanning microscope imaging system, and the acquisition card performs the scanning of the laser scanning microscope according to the line synchronization signal, frame synchronization signal and pixel sampling clock signal. The gray value signal of the sample in the photon counter of the imaging system is collected point by point, and the collected image is sent to a computer to reconstruct a real-time video image point by point.

The laser scanning microscopic imaging system includes a second laser, a first reflector, a polarizing beam splitter, a polygon reflector, a first lens, a second lens, a galvanometer vibrating mirror, a third lens, a fourth lens, four One-third wave plate, microscope objective lens, the fifth lens, the second reflector, confocal pinhole, photon counter, acquisition card and computer, the galvanometer oscillating mirror is placed on the same plane as the multi-faceted reflector object on the image plane of the yoke; the laser light emitted by the second laser is reflected to the polarized beam splitter by the first reflector, and the linearly polarized light emitted by the polarized beam splitter is emitted to the polygonal reflector, The light quickly scanned by the polygon mirror is sent to the telescope lens group composed of the first lens and the second lens, and the light emitted by the telescope lens group is sent to the galvanometer oscillating mirror. The light emitted by the galvanometer of the current meter is transmitted to the quarter-wave plate through the telescope lens group composed of the third lens and the fourth lens, and the quarter-wave plate converts linearly polarized light into circularly polarized light Afterwards, it is focused onto the sample through the microscope objective lens, and the reflected light from the sample is sent back to the quarter wave plate through the microscope objective lens, and the quarter wave plate converts the circularly polarized light into The polarized light at 90° to the illuminating light propagates sequentially along the fourth lens, the third lens, the galvanometer vibrating mirror, the second lens and the first lens, and is reflected to the polarized light by the polygon mirror. A beam splitter, the light emitted by the polarizing beam splitter sequentially passes through the fifth lens and the second mirror to the confocal aperture and is detected and received by the photon counter, and the acquisition card collects the photons The signal from the counter is sent to the computer to reconstruct the real-time video image.

The capture card is one of a non-standard video capture card and a standard video capture card.

The amplification modulation circuit includes an amplifier and a comparator, the output terminal of the photodiode is connected to the input terminal of the amplifier, the output terminal of the amplifier is connected to the input terminal of the comparator, and the output terminal of the comparator is connected to the input to the programmable logic device.

The driving circuit of the galvanometer oscillating mirror includes an operational amplifier, a capacitor and a resistor, the output end of the D/A converter is connected to the inverting terminal of the operational amplifier, and the output end of the operational amplifier is connected to the galvanometer oscillating mirror The control board is driven, a capacitor is connected between the output terminal of the operational amplifier and the inverting terminal, and the non-inverting terminal of the operational amplifier is grounded through a resistor; the operational amplifier is an operational amplifier.

Because the present invention adopts the above technical scheme, it has the following advantages: 1. The present invention includes a laser scanning microscopic imaging system, a first laser, a photodiode and a confocal microscopic imaging control system, and the confocal microscopic imaging control system includes a programmable Logic device, amplification modulation circuit, D/A converter and galvanometer oscillating mirror drive circuit, programmable logic device includes counting frequency division module and vertical synchronous signal generation module, the input terminal of counting frequency division module is connected with crystal oscillator, used for After counting and dividing the crystal oscillator signal, the counting and dividing module sends the pixel sampling clock signal to the multi-faceted mirror to control its rotation. The vertical synchronization signal generation module counts the line synchronization signal according to actual needs to generate a frame synchronization signal. The acquisition card generates a frame synchronization signal according to the line synchronization. Signal, frame synchronization signal and pixel sampling clock signal collect the gray value signal of the sample in the photon counter point by point, and send the collected image to the computer to reconstruct the real-time video image point by point, so the present invention divides the frequency by counting The module controls the multi-faceted mirror, and counts the line synchronization signal to generate a frame synchronization signal through the vertical synchronization signal generation module. It can realize different resolutions and scanning frame rates according to the experimental needs without changing the circuit, and realizes the imaging rate. Adjustable for great flexibility. 2. The capture card of the present invention can adopt non-standard video capture card or standard video capture card to realize imageization according to actual needs. Compared with the prior art, the present invention provides a kind of multi-choice mode to the user, has strong versatility. 3. The laser scanning microscopic imaging system of the present invention includes other optical devices such as a multi-faceted reflector, a galvanometer, and some lenses. The movement scans the grating lines into a grating surface in the telescope lens group composed of the third lens and the fourth lens, the polygon mirror is used as the fast axis, and the galvanometer is used as the slow axis. Therefore, by controlling the polygon mirror and the galvanometer vibrating mirror Confocal real-time scanning imaging can be achieved by spot scanning. The invention can be widely used in imaging control of laser scanning microscopic imaging system.

Description of drawings

Fig. 1 is a structural schematic diagram of a real-time tunable laser scanning microscopy imaging system of the present invention;

Fig. 2 is the structural representation of the confocal microscopic imaging control system of the present invention;

Fig. 3 is the schematic diagram of the circuit principle of the confocal microscopic imaging control system of the present invention;

Fig. 4 is a schematic diagram of the signal flow of the confocal microscopy imaging control system of the present invention.

Detailed ways

The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

The real-time tunable confocal microscopic imaging system of the present invention includes a laser scanning microscopic imaging system, a first laser, a photodiode and a confocal microscopic imaging control system.

As shown in Fig. 1 and Fig. 2, the structure of the laser scanning microscopic imaging system adopted in the present invention is similar to the structure of the laser scanning microscopic imaging system in the prior art, and it includes a second laser 1, a first reflecting mirror 2 , polarizing beam splitter 3, polygon mirror 4, first lens 5, second lens 6, galvanometer vibrating mirror 7, third lens 8, fourth lens 9, quarter-wave plate 10, microscope objective 11. The fifth lens 12, the second reflector 13, the confocal pinhole 14, the photon counter 15, the acquisition card 16 and the computer 17; its working process is: the laser light emitted by the second laser 1 is reflected by the first reflector 2 to The polarized beam splitter 3, the linearly polarized light emitted by the polarized beam splitter 3 is sent to the polygon mirror 4, and the polygon mirror 4 rotates to scan the fast axis (X axis), and the light scanned by the polygon mirror 4 is sent to the The telescope lens group that the first lens 5 and the second lens 6 are formed produces the angular scanning along the grating line in the telescope lens group, and the light emitted by the telescope lens group is sent to the galvanometer oscillating mirror 7, and the galvanometer oscillating mirror 7 Slow-axis (Y-axis) scanning, the light emitted by the galvanometer vibrating mirror 7 is transmitted to the quarter-wave plate 10 through the telescope lens group composed of the third lens 8 and the fourth lens 9, and the quarter-wave plate 10 After the linearly polarized light is converted into circularly polarized light, it is focused on the sample through the microscope objective lens 11, and the reflected light from the sample is sent back to the quarter-wave plate 10 through the microscope objective lens 11, and the quarter-wave plate 10 converts the circular polarized light to the sample. The polarized light becomes polarized light at 90° to the illuminating light, and then propagates along the fourth lens 9, the third lens 8, the galvanometer vibrating mirror 7, the second lens 6 and the first lens 5, and passes through the polygon mirror 4 is reflected to the polarizing beam splitter 3, and the light emitted by the polarizing beam splitting mirror 3 sequentially passes through the fifth lens 12 and the second mirror 13 to reach the confocal aperture 14 and is detected and received by the photon counter 15, and the acquisition card 16 collects the photon counter signal and send it to the computer 17 to reconstruct the real-time video image of the sample. Wherein, the second laser 1 can be an infrared laser, and the galvanometer oscillating mirror 7 is placed on the image plane conjugate to the plane of the multi-faceted mirror object 4, and its angular movement makes the grating line in the third lens 8 and the fourth lens 9. A grating surface is scanned in the telescope lens group, so the confocal image of the cross section of the sample can be obtained by controlling the polygonal mirror 4 and the galvanometer galvanometer 7 to perform point scanning and the photon counter 15 .

As shown in Figures 1 to 4, the present invention is characterized in that: it also includes a first laser 18, a photodiode 19 and a confocal microscopic imaging control system, and the confocal microscopic imaging control system includes a programmable logic device 20, an amplification modulation circuit 21. A D/A converter 22 and a galvanometer oscillating mirror drive circuit 23; wherein, the programmable logic device 20 includes a counting frequency division module 201 and a vertical synchronization signal (VSYNC) generation module 202 .

The input terminal of the counting frequency division module 201 is connected with a crystal oscillator 203 (the present invention selects a 20MHz crystal oscillator, and other frequencies can also be selected according to specific requirements, which is not limited here), which is used to generate a square wave signal after counting frequency division of the crystal oscillator signal. The square wave signal is defined as a pixel sampling clock signal (Pixel clk), and the pixel sampling clock signal is sent to the polygonal mirror 4 for controlling the rotation of the polygonal mirror 4, for example: after the counting and dividing module 201 counts and divides the frequency of the 20MHz crystal oscillator signal Generate a 3.333KHz square wave signal to control the rotation speed of the polygon mirror, which can drive the polygon mirror to rotate at a speed of 277.8 revolutions per second.

The laser light emitted by the first laser 18 is reflected by the polygon mirror 4 and received by the photodiode 19. With the rotation of the polygon mirror 4, the photodiode 19 outputs a sinusoidal voltage signal, and sends it to the amplification modulation circuit 21 for processing to generate TTL Pulse signal, the present invention defines a TTL pulse signal as a line synchronization signal (Hsync), and sends it to the programmable logic device 20, and the programmable logic device sends the line synchronization signal to the D/A converter 22, and the D/A conversion Converter 22 converts the line synchronization signal into a current signal, and sends it to the galvanometer oscillating mirror drive circuit 23 for modulation and conversion into a sawtooth wave voltage signal, and sends it to the galvanometer oscillating mirror drive control board to drive the galvanometer The vibrating mirror 7 works;

The vertical synchronous signal generation module 202 of the programmable logic device 20 counts and generates the required frame synchronous signal (Vsync) according to the actual needs of the horizontal synchronous signal, and at the same time, the programmable logic device 20 separately generates the horizontal synchronous signal, the frame synchronous signal and the pixel clock signal Sent to the acquisition card 16, the acquisition card 16 collects the gray value signal of the sample in the photon counter 16 point by point according to the line synchronization signal, the frame synchronization signal and the pixel sampling clock signal, and sends the collected image to the computer 17 for point by point reappearance. Construct a real-time video image.

In above-mentioned embodiment, cause the drift of video image in order to reduce polygon mirror 4 speeds, therefore the present invention uses the rotating speed of polygon mirror as the reference clock source of imaging of the present invention, promptly the acquisition process of line synchronization signal is: the first laser 18 sends The laser light is received by the photodiode 19 after being reflected by one side of the polygon mirror 4. When the incident direction of the laser light is perpendicular to a certain side of the polygon mirror 4, the light intensity received by the photodiode 19 is the strongest at this moment, and the amplitude of the signal generated is Maximum, when the laser light is incident on the polygon mirror 4 through other directions, the light intensity received by the photodiode 19 is weak, and the signal amplitude generated is low. Therefore, with the rotation of the polygon mirror 4, the signal received by the photodiode 19 forms a The sinusoidal voltage signal, because the amplitude of the sinusoidal signal is relatively low, the sinusoidal voltage signal is amplified and modulated into a TTL pulse signal after the amplifying modulation circuit 21, and the period of the TTL pulse signal is exactly the frequency of rotation of the polygon mirror, that is, the fast axis Scanned frequency signal.

In each of the above-mentioned embodiments, as shown in Fig. 3 and Fig. 4, the amplification modulation circuit 21 includes an amplifier 211 and a comparator 212, the output end of the photodiode 19 is connected to the input end of the amplifier 211, and the output end of the amplifier 211 is connected to the comparator The input end of 212, the output end of comparator 212 is connected with the input end of programmable logic device 20, and amplifier 211 can adopt the device or circuit structure in the prior art according to actual needs, and its specific structure is not limited, as long as meet actual needs The magnification is sufficient, and the comparator 211 can be a 74ACT14 comparator, or other comparators according to actual needs, which is not limited here.

In each of the above-mentioned embodiments, as shown in Figure 3, the galvanometer vibrating mirror drive circuit 23 includes an operational amplifier 231, a capacitor 232 and a resistor 233; the output end of the D/A converter 22 is connected to the inverting terminal of the operational amplifier 231, and the operation The output terminal of the amplifier 231 is connected to the galvanometer oscillating mirror drive control board, the output terminal of the operational amplifier 231 is connected to a capacitor between the inverting terminal, and the non-inverting terminal of the operational amplifier 231 is grounded through a resistor 233 . The operational amplifier 231 can be an OP07 operational amplifier, or other operational amplifiers according to actual needs, which is not limited here.

In above-mentioned each embodiment, capture card 16 can adopt non-standard video capture card or standard video capture card according to actual application, if adopt non-standard video capture card, confocal microscopic imaging control system needn't according to the convenience of video when parameter design Regular output has great flexibility; if a standard video capture card is used, such as an output TV card (a kind of standard video capture card) for display, the programmable logic device 20 needs to collect non-standard analog video signals Converted into a standard video signal (such as RS-170, CCIR, NTSC, and PAL video standard) signal output to the computer 17, the image is displayed in real time.

In each of the above-mentioned embodiments, the first laser 18 can be a 650nm laser, or a laser light source of other wavelengths other than infrared rays with better collimation performance can be selected according to actual needs, which is not limited here; the photodiode 19 can be selected according to actual needs. The optical components for the detection function are not limited here; the diameter of the confocal aperture 14 may be on the order of microns, in order to better prevent light from the non-focus plane from entering the photon counter.

The confocal microscopic imaging control system of the present invention will be further described below through specific examples:

If the pixel clock sampling clock signal is set to 10MHZ by the programmable logic device 20, the corresponding line synchronization signal is 10KHZ, and the frame synchronization signal is correspondingly set to be 10HZ, then the present invention works in the mode of 1000×100010fps; Change the corresponding parameters within the allowable range of the rotation speed of the multi-faceted mirror 4, then the pixel sampling clock signal is set to 10MHZ by the programmable logic device 20, the line synchronization signal is 20KHZ, and the frame synchronization signal corresponds to 40HZ, the present invention works at 500 In the mode of ×50040fps; similarly, a higher pixel clock can be selected to change the variable logic device 20 to change the rotation speed of the polygon mirror 4, thereby changing the image resolution and frame rate of the imaging system. However, if such a frame rate cannot meet the requirements when observing intravascular cells, the polygon mirror 4 can be controlled by the programmable logic device 20 to realize an image scanned at a faster frame rate, for example, 40 frames per second with a resolution of 500× 500 images, or 10KHz line scan mode.

In summary, the present invention can realize different resolutions and scan frame rates, as well as different video formats by controlling the polygon mirror 4 through the programmable logic device 20 according to the experimental needs without changing the external control circuit. Output.

The above-mentioned embodiments are only used to illustrate the present invention. All optical devices of the present invention can be positioned using corresponding external brackets during use. The present invention does not limit the specific position of each optical element, which can be carried out according to specific experimental requirements. Adjustment, but the optical path propagation formed by the combination of all optical elements must be consistent with the optical path propagation of the present invention, and meet the requirements of the present invention for irradiation and detection of samples. Any equivalent transformation and improvement based on the technical solution of the present invention are not Should be excluded from the protection scope of the present invention.

Claims (5)

1. a real-time tunable confocal microscopic image system, it is characterized in that: it comprises scan laser microphotograph imaging system, the first laser instrument, photodiode and confocal microscopic image control system, described confocal microscopic image control system comprises PLD, amplification modulating circuit, D/A converter and galvanometer galvanometer drive circuit, described PLD comprises counting frequency division module and vertical synchronizing signal generation module, the input of described counting frequency division module connects a crystal oscillator, for generating pixel sampling clock signal after crystal oscillator signal-count frequency division, described counting frequency division module sends pixel sampling clock signal and is used for controlling its rotation to the polygonal mirror of described scan laser microphotograph imaging system,

The laser that described the first laser instrument sends is received by described photodiode after described polygonal mirror reflection, along with the rotation of described polygonal mirror, described photodiode output sine voltage signal, and send it to described amplification modulating circuit process generate line synchronising signal, and send it to described PLD, described line synchronising signal is sent to described D/A converter by described PLD, described line synchronising signal is converted to current signal by described D/A converter, and send it to described galvanometer galvanometer drive circuit and generate sawtooth voltage signal, and send it to galvanometer galvanometer and drive the galvanometer galvanometer work that drives described scan laser microphotograph imaging system in panel,

The vertical synchronizing signal generation module of described PLD is counted and is produced needed frame synchronizing signal described line synchronising signal according to actual needs, described PLD is by described line synchronising signal simultaneously, described frame synchronizing signal and described pixel sampling clock signal send to respectively the capture card of described scan laser microphotograph imaging system, described capture card is according to line synchronising signal, frame synchronizing signal and pixel sampling clock signal are carried out pointwise collection to the gray value signal of sample in the photon counter of described scan laser microphotograph imaging system, and the image of collection is sent to a computer pointwise reconstruct real-time video image,

Described scan laser microphotograph imaging system comprises second laser, the first reflecting mirror, polarizing beam splitter mirror, polygonal mirror, first lens, the second lens, galvanometer galvanometer, the 3rd lens, the 4th lens, quarter-wave plate, microcobjective, the 5th lens, the second reflecting mirror, confocal aperture, photon counter, capture card and computer, and described galvanometer galvanometer is positioned in the picture plane with described polygonal mirror object plane conjugation; described second laser emitting laser reflexes to described polarizing beam splitter mirror through described the first reflecting mirror, be transmitted into described polygonal mirror through the line polarized light of described polarizing beam splitter mirror outgoing, arrive the telescopic lenses group via described first lens and the second lens composition through the light emission of described polygonal mirror rapid scanning outgoing, the light emission of described telescopic lenses group outgoing is to described galvanometer galvanometer, the light of described galvanometer galvanometer outgoing is transmitted into described quarter-wave plate via the telescopic lenses group of described the 3rd lens and the 4th lens composition, described quarter-wave plate focuses on sample through described microcobjective after transferring line polarized light to circularly polarized light, reflected light through described sample is launched back described quarter-wave plate through described microcobjective, described quarter-wave plate becomes circularly polarized light with illuminating ray and is after the polarized light of 90 ° successively along described the 4th lens, the 3rd lens, galvanometer galvanometer, the second lens and first lens are propagated, and reflex to described polarizing beam splitter mirror through described polygonal mirror, light through described polarizing beam splitter mirror outgoing arrives described confocal aperture and is surveyed and received by described photon counter through described the 5th lens and the second reflecting mirror successively, described capture card gathers the signal of described photon counter and sends it to described computer reconstruction and goes out real-time video image.

2. the real-time tunable confocal microscopic image system of one as claimed in claim 1, is characterized in that: described capture card adopts the one in non-standard video capture card and normal video capture card.

3. the real-time tunable confocal microscopic image system of one as claimed in claim 1 or 2, it is characterized in that: described amplification modulating circuit comprises amplifier and comparator, the outfan of described photodiode connects the input of described amplifier, the outfan of described amplifier connects the input of described comparator, and the outfan of described comparator connects the input of described PLD.

4. the real-time tunable confocal microscopic image system of one as claimed in claim 1 or 2, it is characterized in that: described galvanometer galvanometer drive circuit comprises operational amplifier, electric capacity and resistance, the outfan of described D/A converter connects the end of oppisite phase of described operational amplifier, the outfan of described operational amplifier connects described galvanometer galvanometer and drives panel, between the outfan of described operational amplifier and end of oppisite phase, be connected described electric capacity, the in-phase end of described operational amplifier is by described resistance eutral grounding; Described operational amplifier adopts OP07 operational amplifier.

5. the real-time tunable confocal microscopic image system of one as claimed in claim 3, it is characterized in that: described galvanometer galvanometer drive circuit comprises operational amplifier, electric capacity and resistance, the outfan of described D/A converter connects the end of oppisite phase of described operational amplifier, the outfan of described operational amplifier connects described galvanometer galvanometer and drives panel, between the outfan of described operational amplifier and end of oppisite phase, be connected described electric capacity, the in-phase end of described operational amplifier is by described resistance eutral grounding; Described operational amplifier adopts OP07 operational amplifier.