CN103717611A - 使得在细菌中能够更快地利用蔗糖的变体蔗糖转运蛋白多肽 - Google Patents

使得在细菌中能够更快地利用蔗糖的变体蔗糖转运蛋白多肽 Download PDF

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Publication number
CN103717611A
CN103717611A CN201280039390.2A CN201280039390A CN103717611A CN 103717611 A CN103717611 A CN 103717611A CN 201280039390 A CN201280039390 A CN 201280039390A CN 103717611 A CN103717611 A CN 103717611A
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Prior art keywords
sucrose
seq
amino acid
polypeptide
changing
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Pending
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CN201280039390.2A
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English (en)
Chinese (zh)
Inventor
Q.陈
Q.程
J.P.赖
K.吕布林-贾斯
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EIDP Inc
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EI Du Pont de Nemours and Co
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Publication of CN103717611A publication Critical patent/CN103717611A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN201280039390.2A 2011-08-16 2012-08-16 使得在细菌中能够更快地利用蔗糖的变体蔗糖转运蛋白多肽 Pending CN103717611A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/210,488 2011-08-16
US13/210,488 US8629243B2 (en) 2011-08-16 2011-08-16 Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria
PCT/US2012/051222 WO2013025945A2 (en) 2011-08-16 2012-08-16 Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria

Publications (1)

Publication Number Publication Date
CN103717611A true CN103717611A (zh) 2014-04-09

Family

ID=47712908

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280039390.2A Pending CN103717611A (zh) 2011-08-16 2012-08-16 使得在细菌中能够更快地利用蔗糖的变体蔗糖转运蛋白多肽

Country Status (9)

Country Link
US (2) US8629243B2 (2)
EP (1) EP2744818A4 (2)
JP (1) JP2014525239A (2)
KR (1) KR20140054233A (2)
CN (1) CN103717611A (2)
AU (1) AU2012296494A1 (2)
BR (1) BR112014003600A2 (2)
IN (1) IN2014DN00161A (2)
WO (1) WO2013025945A2 (2)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105779404A (zh) * 2016-05-11 2016-07-20 广西大学 一种蔗糖磷酸化酶的突变体t155s及其应用
CN110241097A (zh) * 2019-05-24 2019-09-17 山东大学 Pet降解酶及其突变体和应用

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8686114B2 (en) * 2012-03-05 2014-04-01 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides
US9017961B2 (en) 2012-03-05 2015-04-28 E.I. Du Pont De Nemours And Company Recombinant bacteria comprising novel sucrose transporters
KR101588862B1 (ko) 2015-01-19 2016-01-26 (주)제이앤푸드 수용성 보리차 분말의 제조 방법
CN105331618A (zh) * 2015-11-16 2016-02-17 华南农业大学 一种小桐子蔗糖转运蛋白同源基因的克隆方法
CN107236754B (zh) * 2016-03-28 2021-02-05 中国科学院青岛生物能源与过程研究所 利用聚球藻utex2973生产糖类化合物的构建体、菌株与方法
US11390889B2 (en) 2018-10-30 2022-07-19 Green Earth Institute Co., Ltd. Method for manufacturing 1,3-propanediol
WO2022129470A1 (en) * 2020-12-18 2022-06-23 Inbiose N.V. Variant sucrose permease polypeptides
WO2023168244A1 (en) * 2022-03-03 2023-09-07 Cargill, Incorporated Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate
WO2023168233A1 (en) * 2022-03-03 2023-09-07 Cargill, Incorporated Genetically modified yeast and fermentation processes for the production of 3-hydroxypropionate

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007041269A2 (en) * 2005-09-29 2007-04-12 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols
US20100029489A1 (en) * 1998-04-09 2010-02-04 E.I. Du Pont De Nemours And Company Sucrose transport proteins
US20110126318A1 (en) * 2009-11-23 2011-05-26 E.I. Dupont De Nemours And Company Sucrose transporter genes for increasing plant seed lipids
WO2011069033A1 (en) * 2009-12-04 2011-06-09 E. I. Du Pont De Nemours And Company Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2212447C2 (ru) 2000-04-26 2003-09-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Штамм escherichia coli - продуцент аминокислоты (варианты) и способ получения аминокислот (варианты)
CA2522928C (en) 2003-05-06 2013-09-24 E.I. Du Pont De Nemours And Company Purification of biologically-produced 1,3-propanediol
ES2424240T3 (es) 2007-12-18 2013-09-30 Korea Advanced Institute Of Science And Technology Microorganismo recombinante con capacidad de utilizar sacarosa como fuente de carbono
MX2011004842A (es) 2008-11-07 2011-05-30 Metabolic Explorer Sa Uso de sacarosa como sustrato para la produccion fermentativa de 1,2-propanodiol.

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100029489A1 (en) * 1998-04-09 2010-02-04 E.I. Du Pont De Nemours And Company Sucrose transport proteins
WO2007041269A2 (en) * 2005-09-29 2007-04-12 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols
US20110126318A1 (en) * 2009-11-23 2011-05-26 E.I. Dupont De Nemours And Company Sucrose transporter genes for increasing plant seed lipids
WO2011062748A1 (en) * 2009-11-23 2011-05-26 E.I. Du Pont De Nemours And Company Sucrose transporter genes for increasing plant seed lipids
WO2011069033A1 (en) * 2009-12-04 2011-06-09 E. I. Du Pont De Nemours And Company Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105779404A (zh) * 2016-05-11 2016-07-20 广西大学 一种蔗糖磷酸化酶的突变体t155s及其应用
CN110241097A (zh) * 2019-05-24 2019-09-17 山东大学 Pet降解酶及其突变体和应用
CN110241097B (zh) * 2019-05-24 2022-05-17 山东大学 Pet降解酶突变体及其应用

Also Published As

Publication number Publication date
KR20140054233A (ko) 2014-05-08
US8629243B2 (en) 2014-01-14
IN2014DN00161A (2) 2015-05-22
US20140093927A1 (en) 2014-04-03
BR112014003600A2 (pt) 2018-04-17
JP2014525239A (ja) 2014-09-29
WO2013025945A2 (en) 2013-02-21
US20130045518A1 (en) 2013-02-21
EP2744818A2 (en) 2014-06-25
WO2013025945A3 (en) 2013-04-25
AU2012296494A1 (en) 2014-01-23
EP2744818A4 (en) 2014-12-31

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Application publication date: 20140409