CN103736070B - Xiangsha (Chinese character) oral emulsion for nourishing stomach and preparation method thereof - Google Patents

Abstract

The invention discloses a xiangsha (Chinese character) oral emulsion for nourishing the stomach, comprising the following components: (i) 60-85% of xiangsha stomach-nourishing soup and 0.5-1.2% of volatile oil, (ii) 1.0-15% of medicinal oil, (iii) 3.0-25% of emulsifier, (iv) 0-10% of auxiliary emulsifying agent, (v) 0.005-1.0% of antioxidant, (vi) 0-1.5% of preservative and the balance of water. The xiangsha oral emulsion for nourishing the stomach is simple in preparation technology, low in production cost, good in stability and high in bioavailability.

Description

Oral emulsion for nourishing the stomach with fragrant sand and its preparation method

technical field

The invention relates to the field of traditional Chinese medicine preparations, in particular to an oral emulsion for nourishing the stomach with fragrant sand and a preparation method thereof.

Background technique

Xiangsha Yangwei Pill is a variety recorded in the first part of the Chinese Pharmacopoeia in 2000. It is widely used in clinical practice. Its main function is to warm the middle and stomach. The prescription is composed of 14 traditional Chinese medicines: woody, Amomum, Atractylodes macrocephala, tangerine peel, poria, pinellia, Cyperus cyperi, Citrus aurantium, cardamom, Magnolia officinalis, patchouli, licorice, ginger, and jujube. Among them, woody, Amomum, Atractylodes macrocephala, tangerine peel, Cyperus cyperi, Citrus aurantium, cardamom, Magnolia officinalis, Patchouli, and ginger have high volatile oil content, which can relieve gastrointestinal smooth muscle spasm, regulate qi, and warm Stomach digestion, promote the secretion of gastric juice, enhance the efficacy of digestion, is the main active ingredient of the preparation.

Xiangsha Yangwei Oral Liquid and Xiangsha Yangwei Soft Capsules are recorded in the twentieth volume of "Drug Standards Traditional Chinese Medicine Prescriptions" published by the Ministry of Health of the People's Republic of China (Pharmacopoeia Committee of the Ministry of Health of the People's Republic of China, 1998). On the basis of the traditional Chinese medicine preparation Xiangsha Yangwei Pills and the above records, this field has improved and obtained a series of Xiangsha Yangwei Granules, Xiangsha Yangwei Capsules, Xiangsha Yangwei Tablets, Xiangsha Yangwei Oral Liquid, etc. Stomach preparations. However, in these fragrant sand nourishing stomach products, the release of active components of traditional Chinese medicine is insufficient, the absorption is limited, the bioavailability is low, and the traditional Chinese medicine components therein have a great bad smell, and the acceptance degree of consumers is poor.

Therefore, there is still a need to further develop other preparation forms of fragrant sand nourishing the stomach, to overcome the shortcomings of the above-mentioned fragrant sand nourishing the stomach product.

Emulsion is a common dosage form in pharmacy. Generally, it is oil-in-water type (O/W), and there is also water-in-oil type (W/O). W). Oral emulsions are good carriers for gastrointestinal administration and have many advantages, including:

1. The emulsion has good dispersion effect and large effective surface area, which is beneficial to the release, dissolution and absorption of drugs;

2. The emulsion contains an emulsifier, which has a surface active effect, can improve the performance of the gastrointestinal mucosa, and promote drug absorption;

3. After the oil in the emulsion is absorbed, it can promote the secretion of bile, increase the flow rate of blood and lymph, and help the dissolution and absorption of drugs. The oily substances in the emulsion may also be transported and absorbed through the lymphatic system;

4. The fat in the emulsion is digested to generate linoleic acid and oleic acid, which can inhibit the peristalsis of the gastrointestinal tract and prolong the residence time of the drug in the small intestine;

5. Oral emulsions can ensure accurate dosage and are convenient to take. Oil-in-water emulsions can cover the bad smell of fat-soluble drugs, and can also add flavoring agents;

6. The emulsion can locate the drug in the oil-water interface film or the nucleus of the oil droplet, avoiding the direct contact of the drug with the gastrointestinal tract, thereby reducing the irritation of the drug to the gastrointestinal tract, and avoiding the common gastrointestinal reactions commonly seen in oral administration of general drugs, such as Nausea, vomiting, stomach burning, epigastric pain, and severe cases can lead to extreme gastrointestinal bleeding and gastric ulcers.

However, the preparation of emulsions generally requires the use of various types of surfactants that can effectively reduce the interfacial tension, so that the surface tension of the micro-droplets formed by emulsification is reduced enough, so that the internal phase of the micro-droplets is no longer broken by the interface. And the internal phase of other tiny droplets in the external phase is fused, so as to form a physically stable two-phase or multi-phase system in which oil and water are evenly dispersed. Therefore, the composition of an emulsion has a great influence on its stability and bioavailability. At present, there is no disclosure of Xiangsha nourishing stomach oral emulsion in the prior art.

Contents of the invention

The purpose of the present invention is to provide a low-cost, simple preparation method and higher bioavailability Xiangsha Nourishing Stomach Oral Emulsion, which is used for the treatment of stomach pain, fullness caused by deficiency of stomach yang, dampness and stagnation of Qi. Stomach pain, nausea, vomiting, sour water, noisy discomfort, lack of appetite, fatigue of limbs.

The present invention achieves the above-mentioned technical purpose through the following technical solutions.

An oral emulsion for nourishing the stomach with fragrant sand, which contains the following ingredients:

(i) 60-85% Xiangsha Nourishing Stomach Liquid and 0.5-1.2% volatile oil;

(ii) 1.0-15% medicinal oil;

(iii) 3.0-25% emulsifier;

(iv) 0-10% auxiliary emulsifier;

(v) 0.005-1.0% antioxidant;

(vi) 0-1.5% preservatives;

remaining water;

The preparation method of the fragrant sand nourishing stomach medicine liquid and volatile oil is as follows: take 70 g of woody root, 70 g of amomum, 100 g of Atractylodes macrocephala, 100 g of tangerine peel, 100 g of poria cocos, 100 g of pinellia (made by) cyperus cyperi (made of vinegar) ) 70 g, citrus aurantium (fried) 70 g, cardamom (shelled) 70 g, magnolia officinalis (ginger) 70 g, patchouli 70 g, licorice 30 g, ginger 30 g and jujube 50 g Volatile oil, collect the volatile oil for later use, add water to decoct the remaining medicinal residues 2-3 times, 45-75 minutes each time, combine the decoction, filter, and concentrate the filtrate to a clear paste with a relative density of 1.14-1.18 at 75°C, add ethanol to make the paste containing The alcohol content is 70-85%, let it stand for 40-50 hours, take the supernatant to recover ethanol, add appropriate amount of water to the remaining liquid medicine, adjust the pH value to 3.5-4.5, let it stand for 40-50 hours, filter, and get the fragrant sand Stomach nourishing liquid.

Wherein, the unit of the above percentage is % (g/100ml).

Preferably, the medicinal oil is selected from one or more of natural vegetable oil, ethyl oleate, butyl oleate, long-chain triglycerides or medium-chain triglycerides.

Preferably, the emulsifier is selected from fatty acid sorbitan, polyoxyethylene fatty acid ester, polyoxyethylene fatty alcohol ether, polyoxyethylene-polyoxypropylene copolymer, polyoxyethylene-polyoxypropylene block copolymer Species, monoglyceride fatty acid ester, triglyceride fatty acid ester, polyglycerol fatty acid ester, sucrose fatty acid ester, glyceryl monostearate, sodium stearate, potassium stearate, sodium oleate, potassium oleate , calcium stearate, sodium lauryl sulfate, cetyl sulfated castor oil, gum arabic, gum tragacanth, gelatin or phospholipids.

Preferably, the auxiliary emulsifier is selected from the group consisting of methyl cellulose, sodium carboxymethyl cellulose, carboxypropyl cellulose, sodium alginate, agar, gum tragacanth, gum arabic, xanthan gum, guar gum, One or more of pectin, bentonite, cetyl alcohol, beeswax, glyceryl monostearate, stearic acid, stearyl alcohol, glycerin, polyethylene glycol, polyglycerol esters or povidone.

Preferably, the antioxidant is selected from sodium sulfite, sodium bisulfite, sodium thiosulfate, sodium metabisulfite, ascorbic acid, propyl gallate, ascorbyl palmitate, tert-butyl p-hydroxyanisole, di-tert-butyl p- One or more of cresol, vitamin E or vitamin E acetate.

Preferably, the preservative is selected from one or more of benzoic acid, calcium sorbate, ethylparaben, propylparaben, sodium benzoate or potassium sorbate.

Preferably, the water is deionized water or water for injection.

More preferably, the medicinal oil in the present invention is soybean oil, castor oil, peanut oil, sesame oil, corn oil, olive oil, tea oil, ethyl oleate, butyl oleate, long-chain triglyceride or medium-chain glycerol One or more of the triesters, the dosage is 2.5-9.0%. Wherein, the long-chain triglycerides refer to triglycerides of fatty acids with 14-22 carbon atoms, and the medium-chain triglycerides refer to triglycerides of fatty acids with 8-12 carbon atoms.

More preferably, the emulsifier in the present invention is Tween 80, polyethylene glycol-12-hydroxystearate (HS-15), poloxamer 188, soybean lecithin, sodium lauryl sulfate, poly One or more of oxyethylene hydrogenated castor oil (RH40) or polyoxyethylene-35-castor oil (EL-35), the dosage is 3.0-11.0%.

More preferably, the auxiliary emulsifier in the present invention is selected from one or more of sodium carboxymethylcellulose, glyceryl monostearate or povidone, and the dosage is 0.05-3.5%.

More preferably, the antioxidant in the present invention is selected from sodium sulfite, sodium bisulfite, sodium thiosulfate, sodium metabisulfite, ascorbic acid, propyl gallate, ascorbyl palmitate, tert-butyl p-hydroxyanisole, dibutyl One or more of cresol, vitamin E or vitamin E acetate, the dosage is 0.01-0.5%.

More preferably, the preservative in the present invention is selected from one or more of ethylparaben, propylparaben, sodium benzoate or potassium sorbate, and the dosage is 0.01-0.8%.

In addition, the emulsion of the present invention also contains pharmaceutically acceptable flavoring agents and fragrances.

The fragrant sand nourishing stomach oral emulsion of the present invention can be prepared by the following method, and the steps include: taking volatile oil, medicinal oil, antioxidant and preservative and mixing uniformly to obtain an oil phase, keeping it warm at 40-80°C for later use, taking the incense Sand nourishing stomach medicinal liquid, emulsifier, auxiliary emulsifier and water are mixed and dissolved, then added to the oil phase, magnetically stirred at 40-80°C for 10-60 minutes, sheared at 5000-8000 rpm for 1-30 minutes, poured Seal, sterilize, and get ready.

Alternatively, it can be prepared by the following method, the steps of which include: taking volatile oil, medicinal oil, antioxidant and preservative and mixing evenly to obtain an oil phase, keeping it warm at 40-80°C for later use, taking fragrant sand nourishing stomach liquid, emulsifying Add the agent, auxiliary emulsifier and water to the oil phase after mixing and dissolving, disperse at 100 W to 1000 W for 1 to 5 min through an ultrasonic emulsifier, repeat 3 to 4 times, potting, and sterilizing.

The fragrant sand nourishing stomach oral emulsion of the present invention can also be prepared by the following method, the steps of which include: taking volatile oil, medicinal oil, antioxidant and preservative, mixing uniformly, preheating at 40-80°C, and preparing the oil phase for later use; Under slow stirring, add the water phase preheated at 40-80 ℃ containing fragrant sand stomach nourishing liquid, emulsifier, auxiliary emulsifier and water into the preheated oil phase in the form of fine jets, potting, Sterilize, that is.

Compared with the prior art, the technical solution of the present invention has the following advantages:

1. The present invention extracts the active ingredients in the traditional Xiangsha Yangwei Pills and makes an oral emulsion, which makes the release of the active ingredients in the Xiangsha Yangwei Chinese medicine composition more sufficient, promotes absorption, and greatly improves bioavailability.

2. The present invention utilizes the characteristics of the emulsion through the extraction process to effectively cover the bad smell of the traditional Chinese medicine components, has a good taste, and improves the acceptance of consumers.

3. The present invention rationally selects medicinal oils, emulsifiers and other auxiliary materials and their proportions, so that the prepared Xiangsha Nourishing Stomach Oral Emulsion has good stability and is suitable for storage.

Detailed ways

The present invention provides the following examples to further illustrate, but the protection scope of the present invention is not limited to these examples.

Example 1: Preparation of fragrant sand nourishing stomach medicinal liquid and volatile oil

Take 70g Muxiang, 70g Amomum, 100g Atractylodes Atractylodes, 100g Chenpi, 100g Poria, 100g Pinellia (made from vinegar), 70g Cyperus cyperi (made from vinegar), 70g Zhishi (stir-fried), cardamom (removed) shell) 70 g, magnolia officinalis (made from ginger) 70 g, patchouli 70 g, licorice 30 g, ginger 30 g and jujube 50 g to extract volatile oil together, collect the volatile oil for later use, and decoct the remaining medicinal residue with water twice, 50 minutes each time, combine the decoction, filter, concentrate the filtrate to a clear paste with a relative density of 1.16 at 75°C, add ethanol to make the alcohol content 80%, let it stand for 45 hours, take the supernatant to recover the ethanol, and the remaining liquid Add appropriate amount of water, adjust the pH value to 4.0, let it stand for 45 hours, and filter to obtain the fragrant sand nourishing stomach liquid.

Example 2: Preparation of fragrant sand nourishing stomach medicinal liquid and volatile oil

Take 70g Muxiang, 70g Amomum, 100g Atractylodes Atractylodes, 100g Chenpi, 100g Poria, 100g Pinellia (made from vinegar), 70g Cyperus cyperi (made from vinegar), 70g Zhishi (stir-fried), cardamom (removed) shell) 70 g, magnolia officinalis (made from ginger) 70 g, patchouli 70 g, licorice 30 g, ginger 30 g and jujube 50 g to extract volatile oil together, collect the volatile oil for later use, and decoct the remaining medicinal residue with water for 3 times, 60 minutes each time, combine the decoction, filter, concentrate the filtrate to a clear paste with a relative density of 1.18 at 75°C, add ethanol to make the alcohol content 80%, let it stand for 48 hours, take the supernatant to recover the ethanol, and the remaining liquid medicine Add an appropriate amount of water to adjust the pH to 4.2, let it stand for 48 hours, and filter to obtain the fragrant sand nourishing stomach liquid.

Example 3: Preparation of Xiangsha Nourishing Stomach Oral Emulsion

prescription:

The fragrant sand stomach nourishing medicinal liquid of embodiment 1 80% (g/100ml) The volatile oil of embodiment 1 0.9% (g/100ml) long chain triglycerides 3% (g/100ml) Soy lecithin 9% (g/100ml) tert-butyl p-hydroxyanisole 0.5% (g/100ml) Ethylparaben 0.05% (g/100ml) Propylparaben 0.05% (g/100ml) Water for Injection margin

Preparation method: Mix volatile oil, long-chain triglycerides, tert-butyl-p-hydroxyanisole, ethylparaben and propylparaben evenly to obtain an oil phase, keep warm at 60°C for later use; Liquid, soybean lecithin and water for injection are mixed and dissolved, then added to the oil phase, magnetically stirred at 60°C for 30 minutes, cut with a high-speed shearer at a speed of 6000 rpm for 15 minutes, potted and sterilized to obtain an oral emulsion .

Example 4: Preparation of Xiangsha Nourishing Stomach Oral Emulsion

prescription:

The fragrant sand stomach nourishing medicinal liquid of embodiment 1 60% (g/100ml) The volatile oil of embodiment 1 0.7% (g/100ml) Soybean oil 5% (g/100ml) Polyethylene glycol-12-hydroxystearate 6% (g/100ml) Tween 80 2% (g/100ml) Vitamin E Acetate 0.5% (g/100ml) Ethylparaben 0.2% (g/100ml) essence 0.15% (g/100ml) Water for Injection margin

Preparation:

Mix volatile oil, soybean oil, vitamin E acetate and ethylparaben evenly to obtain an oil phase, and keep it warm at 50°C for later use; mix fragrant sand stomach nourishing liquid, polyethylene glycol-12-hydroxystearate, Tween-80, essence and water for injection are mixed and dissolved, then added to the oil phase, magnetically stirred at 50°C for 40 minutes, cut with a high-speed shearer at a speed of 7000 rpm for 12 minutes, potted and sterilized to obtain Oral emulsion.

Example 5: Preparation of Xiangsha Nourishing Stomach Oral Emulsion

prescription:

The fragrant sand stomach nourishing medicinal liquid of embodiment 1 82% (g/100ml) The volatile oil of embodiment 1 0.8% (g/100ml) Butyl oleate 4% (g/100ml) Soy lecithin 6% (g/100ml) Polyoxyethylene Hydrogenated Castor Oil 3% (g/100ml) Ascorbyl Palmitate 0.5% (g/100ml) sodium benzoate 0.1% (g/100ml) Deionized water margin

Preparation:

Mix the volatile oil, butyl oleate, ascorbyl palmitate and sodium benzoate evenly to obtain the oil phase, keep it warm at 70°C for later use; mix the fragrant sand stomach nourishing liquid, soybean lecithin, polyoxyethylene hydrogenated castor oil and deionized water After dissolving, add it to the oil phase, disperse it at 800W for 5 minutes through an ultrasonic emulsifier, repeat 3 times, potting, and sterilizing it.

Example 6: Preparation of Xiangsha Nourishing Stomach Oral Emulsion

prescription:

The fragrant sand stomach nourishing medicinal liquid of embodiment 1 75% (g/100ml) The volatile oil of embodiment 1 1.0% (g/100ml) medium chain triglycerides 8% (g/100ml) Polyoxyethylene-35-castor oil 3.5% (g/100ml) Propyl gallate 0.5% (g/100ml) Potassium sorbate 0.8% (g/100ml) Water for Injection margin

Preparation:

Mix the volatile oil, medium-chain triglyceride, propyl gallate and potassium sorbate evenly to obtain the oil phase, and keep it warm at 80°C for later use; mix the fragrant sand stomach nourishing liquid, polyoxyethylene-35-castor oil and water for injection After dissolving, add it to the oil phase, stir magnetically at 80°C for 20 minutes, cut with a high-speed shearer at a speed of 8000 rpm for 10 minutes, fill and sterilize to obtain an oral emulsion.

Example 7: Preparation of Xiangsha Nourishing Stomach Oral Emulsion

prescription:

The fragrant sand stomach nourishing medicinal liquid of embodiment 1 85% (g/100ml) The volatile oil of embodiment 1 0.6% (g/100ml) Corn oil 7% (g/100ml) Soy lecithin 5% (g/100ml) Poloxamer 188 0.1 (g/100ml) Sodium metabisulfite 0.1 % (g/100ml) Deionized water margin

Preparation:

Mix the volatile oil, corn oil and sodium metabisulfite evenly to prepare the oil phase, and keep it warm at 60°C for later use; mix and dissolve Xiangsha nourishing stomach liquid, soybean lecithin, poloxamer 188 and deionized water and add it to the oil phase. Stir magnetically at 60°C for 40 minutes, cut with a high-speed shearer at a speed of 6,000 rpm for 20 minutes, potting, and sterilizing to obtain an oral emulsion.

Example 8: Preparation of Xiangsha Nourishing Stomach Oral Emulsion

prescription:

The fragrant sand nourishing stomach medicinal liquid of embodiment 2 80% (g/100ml) The volatile oil of embodiment 2 0.9% (g/100ml) tea oil 3% (g/100ml) Polyethylene glycol-12-hydroxystearate 7% (g/100ml) sodium bisulfite 0.2% (g/100ml) Sodium carboxymethyl cellulose 0.15% (g/100ml) Water for Injection margin

Preparation:

Mix the volatile oil, camellia oil and sodium bisulfite evenly to obtain the oil phase, and keep it warm at 70°C for later use; mix the fragrant sand stomach nourishing liquid, polyethylene glycol-12-hydroxystearate, sodium carboxymethyl cellulose Mix and dissolve with water for injection, add to the oil phase, stir magnetically at 70°C for 25 minutes, cut with a high-speed shearer at a speed of 5,000 rpm for 20 minutes, potting, and sterilizing to obtain an oral emulsion.

Example 9: Preparation of Xiangsha Nourishing Stomach Oral Emulsion

prescription:

The fragrant sand nourishing stomach medicinal liquid of embodiment 2 65% (g/100ml) The volatile oil of embodiment 2 1.2% (g/100ml) ethyl oleate 4.5% (g/100ml) Poloxamer 188 8.5 (g/100ml) Tween 80 2% (g/100ml) Glyceryl monostearate 3.5% (g/100ml) Dibutylcresol 0.01% (g/100ml) Water for Injection margin

Preparation:

Mix the volatile oil, ethyl oleate and butylcresol evenly, and preheat to prepare the oil phase for later use; under slow stirring, use the method of fine jet flow to mix the medicinal liquid containing fragrant sand, poloxamer 188, The water phase of Tween-80, glyceryl monostearate and water for injection is added to the preheated oil phase, filled and sterilized to obtain the product.

Example 10: Preparation of Xiangsha Nourishing Stomach Oral Emulsion

prescription:

The fragrant sand nourishing stomach medicinal liquid of embodiment 2 78% (g/100ml) The volatile oil of embodiment 2 0.8% (g/100ml) castor oil 8.7% (g/100ml) Sodium dodecyl sulfate 2% (g/100ml) Polyethylene glycol-12-hydroxystearate 1% (g/100ml) Vitamin E 0.5% (g/100ml) Povidone 0.05% (g/100ml) Water for Injection margin

Preparation:

Mix volatile oil, castor oil and vitamin E evenly to obtain an oil phase, and keep it warm at 60°C for later use; mix fragrant sand stomach nourishing liquid, sodium lauryl sulfate, polyethylene glycol-12-hydroxystearate, poly Mix and dissolve the vitamin ketone and water for injection, add it to the oil phase, stir magnetically at 60°C for 30 minutes, cut with a high-speed shearer at a speed of 8,000 rpm for 15 minutes, fill and sterilize to obtain an oral emulsion.

Example 11: Stability test of Xiangsha nourishing stomach oral emulsion

1. Test samples:

The Xiangsha nourishing stomach oral emulsion prepared by the method of Example 3.

2. Test method:

Refer to the guidelines for the stability test of pharmaceutical preparations (Appendix II of the Chinese Pharmacopoeia 2010 Edition C), carry out accelerated experimental investigation on this product, and provide scientific basis for the transportation, packaging, storage, and validity period of this product. The general rules stipulate that the oral emulsion should be tested under the conditions of temperature 30±2°C and humidity 65±5%.

In this study, three batches of samples were prepared respectively, and the stability was investigated under the conditions of 45±2°C, humidity 65±5%, 30±2°C, humidity 65±5%, and room temperature. Sampling to investigate properties, pH value, average particle size and content. The results are shown in Table 1.

3. Test results:

Table 1 The results of the stability investigation of the embodiment of the present invention 3 Xiangsha nourishing stomach emulsion

The results show that the Xiangsha Nourishing Stomach Emulsion of Example 3 of the present invention was stored at 45±2°C, humidity 65±5%, 30±2°C, humidity 65±5%, and room temperature for 6 months, and the indicators were not obvious. Change, showing that the emulsion of the present invention has good stability.

Example 12: Drug efficacy test of Xiangsha nourishing stomach oral emulsion

1. Test sample:

The Xiangsha nourishing stomach oral emulsion prepared by the method of Example 3.

2. Test methods and results:

1. Effect of Xiangsha Nourishing Stomach Emulsion on the secretion of gastric juice in rats

40 male SD rats were randomly divided into 4 groups, namely blank control group, Xiangsha Yangwei oral emulsion high-dose group, middle-dose group and low-dose group, ig administration, once a day, for 7 consecutive days day, fasted for 12 hours after the last administration, anesthetized with ether, and ligated the pylorus. Animals were sacrificed after fasting for 10 hours after operation, the cardia was ligated by laparotomy, gastric juice was collected, and the gastric juice volume, pH value and pepsin activity were measured. The results are shown in Table 2.

Table 2 The effect of Xiangsha nourishing stomach emulsion on the secretion of gastric juice in rats ( ± s)

Note * means compared with the blank control group, P<0.05, ** means compared with the blank control group, P<0.01

The results showed that Xiangsha Nourishing Stomach Emulsion can significantly reduce the secretion of gastric juice, increase the pH value of gastric juice, and increase the activity of pepsin (P<0.01) or (P<0.05).

2. Effect of Xiangsha Nourishing Stomach Emulsion on Small Intestine Propulsion in Mice

40 male Kunming mice were randomly divided into 4 groups, namely blank control group, Xiangsha nourishing stomach emulsion high-dose group, middle-dose group and low-dose group, ig administration, once a day, for 2 consecutive days On the 2nd day after the administration, the mice were fasted but not allowed to drink water for 12 hours and administered once more. After the last administration, 0.2ml of 10% charcoal powder was ig 1, and the mice were sacrificed by cervical dislocation 15 minutes later. Open the abdominal cavity, cut the intestinal tube from the upper end to the lower end of the pylorus to the ileocecal, measure the length of the intestinal tube as the "total length of the small intestine", and the distance from the pylorus to the front of the end of the charcoal is the "distance of the end of the charcoal in the intestine", and use the following formula to calculate the end of the charcoal advance percentage.

Propelling percentage of charcoal powder (%) = propulsion distance of charcoal powder in the intestine / total length of small intestine × 100%

Table 2 The effect of Xiangsha nourishing stomach emulsion on small intestine propulsion in mice ( ± s)

group N Charcoal propulsion percentage (%) Blank control group 10 51.23±8.44 low dose group 10 65.46±12.62 Middle dose group 10 74.59±15.71** high dose group 10 83.19±17.96**

Note * means compared with the blank control group, P<0.05, ** means compared with the blank control group, P<0.01

The results of this test are shown in Table 2. The results showed that Xiangsha Nourishing Stomach Emulsion could significantly increase the propulsion percentage of charcoal in the small intestine (P<0.01).

Claims (6)

1. a cyperus-amomum stomach Orally taken emulsion, is characterized in that containing following composition:

(1) 60-85% cyperus-amomum stomach medicinal liquid and 0.5-1.2% volatile oil;

(2) 2.5-9.0% medicinal oil;

(3) 3.0-11.0% emulsifying agent;

(4) 0.05-3.5% coemulsifier;

(5) 0.01-0.5% antioxidant;

(6) 0.01-0.8% antiseptic;

The water of surplus;

The preparation method of described cyperus-amomum stomach medicinal liquid and volatile oil is: get Radix Aucklandiae 70g, Fructus Amomi 70 g, the Rhizoma Atractylodis Macrocephalae 100 g, Pericarpium Citri Reticulatae 100 g, Poria 100 g, Rhizoma Pinelliae Preparata 100 g, Rhizoma Cyperi (processed with vinegar) 70 g, Fructus Aurantii Immaturus (parched) 70 g, Semen Myristicae (shell removed) 70 g, Sichuan Cortex Magnoliae Officinalis (processed with Rhizoma Zingiberis Recens) 70 g, Herba Pogostemonis 70 g, Radix Glycyrrhizae 30 g, Rhizoma Zingiberis Recens 30 g and Fructus Jujubae 50 g extract volatile oil in the lump, volatile oil is collected standby, residue medicinal residues decoct with water 2-3 time, each 45-75 minute, collecting decoction, filter, filtrate is concentrated into the clear paste that 75 ℃ of relative densities are 1.14～1.18, add ethanol make containing alcohol amount be 70-85%, standing 40-50 hour, get supernatant and reclaim ethanol, it is appropriate that residue medicinal liquid adds water, adjust pH is 3.5-4.5, standing 40-50 hour, filter, obtain cyperus-amomum stomach medicinal liquid,

Described medicinal oil is one or more in soybean oil, Oleum Ricini, Semen Maydis oil, Oleum Camelliae, ethyl oleate, butyl oleate, long chain triglyceride or medium chain triglyceride; Described emulsifying agent is one or more in Tween 80, HS15, PLURONICS F87, soybean phospholipid, sodium lauryl sulphate, polyoxyethylene hydrogenated Oleum Ricini or polyoxyethylene-35-Oleum Ricini; Described coemulsifier is selected from one or more in sodium carboxymethyl cellulose, polyvidone or glyceryl monostearate; Described antioxidant is selected from one or more in sodium sulfite, sodium pyrosulfite, propyl gallate, ascorbyl palmitate, butylated hydroxyarisol, two fourth cresols, vitamin E or Vitamin E acetate; Described antiseptic is selected from one or more in ethyl hydroxybenzoate, propylparaben, sodium benzoate or potassium sorbate;

The unit of described percentage ratio is %g/100ml.

2. cyperus-amomum stomach Orally taken emulsion as claimed in claim 1, is characterized in that described water is deionized water or water for injection.

3. a cyperus-amomum stomach Orally taken emulsion, is characterized in that also further comprising the acceptable correctives of pharmacy, aromatic on the basis of the cyperus-amomum stomach Orally taken emulsion described in claim 1 or 2.

4. the preparation method of the described cyperus-amomum stomach Orally taken emulsion of one of claim 1-2, it is characterized in that comprising following steps: get volatile oil, medicinal oil, antioxidant and antiseptic mix homogeneously, make oil phase, 40～80 ℃ of insulations are standby, join in oil phase after getting cyperus-amomum stomach medicinal liquid, emulsifying agent, coemulsifier and water mixed dissolution, and 40～80 ℃ of lower magnetic forces stir 10～60min, rotating speed cutting 1～30min with 5000-8000 rev/min, embedding, sterilizing, obtains.

5. the preparation method of the described cyperus-amomum stomach Orally taken emulsion of one of claim 1-2, it is characterized in that comprising following steps: get volatile oil, medicinal oil, antioxidant and antiseptic mix homogeneously, make oil phase, 40～80 ℃ of insulations are standby, join in oil phase after getting cyperus-amomum stomach medicinal liquid, emulsifying agent, coemulsifier and water mixed dissolution, through ultrasonic emulsator, under 100 W～1000 W, disperse 1～5 min, repeat 3～4 times, embedding, sterilizing, obtains.

6. the preparation method of the cyperus-amomum stomach Orally taken emulsion described in one of claim 1-2, is characterized in that comprising following steps: get volatile oil, medicinal oil, antioxidant and antiseptic mix homogeneously, 40～80 ℃ of preheatings, make oil phase standby; Under slowly stirring, in the mode of thin jet, the water that includes cyperus-amomum stomach medicinal liquid, emulsifying agent, coemulsifier and water of 40～80 ℃ of preheatings is joined in the oil phase of preheating, embedding, sterilizing, obtains.