CN105123765A - Preparing method for chitin copper-lactate gray-mold preventing and treating preparation - Google Patents
Preparing method for chitin copper-lactate gray-mold preventing and treating preparation Download PDFInfo
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- CN105123765A CN105123765A CN201510643437.1A CN201510643437A CN105123765A CN 105123765 A CN105123765 A CN 105123765A CN 201510643437 A CN201510643437 A CN 201510643437A CN 105123765 A CN105123765 A CN 105123765A
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- chitin
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- lactate
- gray mold
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- 229920002101 Chitin Polymers 0.000 title claims abstract description 26
- DYROSKSLMAPFBZ-UHFFFAOYSA-L copper;2-hydroxypropanoate Chemical compound [Cu+2].CC(O)C([O-])=O.CC(O)C([O-])=O DYROSKSLMAPFBZ-UHFFFAOYSA-L 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 239000000725 suspension Substances 0.000 claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- 238000000855 fermentation Methods 0.000 claims abstract description 8
- 230000004151 fermentation Effects 0.000 claims abstract description 8
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 7
- 230000000536 complexating effect Effects 0.000 claims abstract description 7
- 229910052802 copper Inorganic materials 0.000 claims abstract description 7
- 239000010949 copper Substances 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 16
- 241000186660 Lactobacillus Species 0.000 claims description 6
- 229940039696 lactobacillus Drugs 0.000 claims description 6
- 244000302544 Luffa aegyptiaca Species 0.000 claims description 4
- 235000009814 Luffa aegyptiaca Nutrition 0.000 claims description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 2
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 2
- 235000007079 manganese sulphate Nutrition 0.000 claims description 2
- 239000011702 manganese sulphate Substances 0.000 claims description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 2
- 231100000252 nontoxic Toxicity 0.000 claims description 2
- 230000003000 nontoxic effect Effects 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 235000009529 zinc sulphate Nutrition 0.000 claims description 2
- 239000011686 zinc sulphate Substances 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 18
- 235000013399 edible fruits Nutrition 0.000 abstract description 15
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 12
- 239000000463 material Substances 0.000 abstract description 8
- 235000014655 lactic acid Nutrition 0.000 abstract description 6
- 239000004310 lactic acid Substances 0.000 abstract description 6
- 239000012530 fluid Substances 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 3
- 244000052769 pathogen Species 0.000 abstract description 3
- 235000009852 Cucurbita pepo Nutrition 0.000 abstract 1
- 241000219104 Cucurbitaceae Species 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 241000220223 Fragaria Species 0.000 description 8
- 235000016623 Fragaria vesca Nutrition 0.000 description 8
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 8
- 239000000575 pesticide Substances 0.000 description 8
- 239000007921 spray Substances 0.000 description 7
- 235000009754 Vitis X bourquina Nutrition 0.000 description 6
- 235000012333 Vitis X labruscana Nutrition 0.000 description 6
- 240000006365 Vitis vinifera Species 0.000 description 6
- 235000014787 Vitis vinifera Nutrition 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 239000003905 agrochemical Substances 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 240000003768 Solanum lycopersicum Species 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 241000235342 Saccharomycetes Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 239000005867 Iprodione Substances 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 229910052789 astatine Inorganic materials 0.000 description 1
- RYXHOMYVWAEKHL-UHFFFAOYSA-N astatine atom Chemical compound [At] RYXHOMYVWAEKHL-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 239000012681 biocontrol agent Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000003958 fumigation Methods 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- ONUFESLQCSAYKA-UHFFFAOYSA-N iprodione Chemical compound O=C1N(C(=O)NC(C)C)CC(=O)N1C1=CC(Cl)=CC(Cl)=C1 ONUFESLQCSAYKA-UHFFFAOYSA-N 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- QXJKBPAVAHBARF-BETUJISGSA-N procymidone Chemical compound O=C([C@]1(C)C[C@@]1(C1=O)C)N1C1=CC(Cl)=CC(Cl)=C1 QXJKBPAVAHBARF-BETUJISGSA-N 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 230000028070 sporulation Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a preparing method for a chitin copper-lactate gray-mold preventing and treating preparation. Materials for the preparing method for the chitin copper-lactate gray-mold preventing and treating preparation are prepared from 80% of plant lactic acid bacterium liquid, 15% of chitin and 5% of nanometer complexing monovalent copper. The preparing method includes the steps that 1, tender leaves of towel gourds are collected to be mixed with brown sugar in the ratio of 2:1, the mixture is placed into a fermentation kettle to be fermented for 7 d, and a suspension obtained after filtering is the plant lactic acid bacterium liquid; 2, the nanometer complexing monovalent copper is slowly put into the suspension obtained in the step 1 till the suspension gets into a cyan color; 3, the chitin is further put into the liquid obtained after the step 2 is completed, stirring is carried out, then standing is carried out for 24 d, and obtained viscous fluid is the chitin copper-lactate gray-mold preventing and treating preparation. When used, the chitin copper-lactate gray-mold preventing and treating preparation is diluted by 500 folds to be sprayed to leaves and fruits of plants, active enzymes of pathogens are damaged, and the gray-mold inhibiting effective rate is 93%.
Description
Technical field
The present invention relates to a kind of preparation method of chitin copper lactate gray mold control agent, belong to the technical field of agro-ecology type control of plant disease.
Background technology
Gray mold is open country, a kind of fungal disease of common and more difficult control in greenhouse gardening, the pathogene of gray mold is that Botrytis cinerea belongs to, happiness grows under the environment of low temperature and high relative humidity, when temperature is at 20-30 DEG C, humidity continues the high-incidence season for disease in the environment of 90%, the flower of plant, really, leaf, stem all can be fallen ill, fruit grows white after infecting, the mould layer of the rear grey having one deck thick, fruit is caused to be the rotten shape of water, gray mold is a kind of typical aeroborne disease, can with air-flow, irrigate or the upper infection of farming operation, infection velocity is fast, as prophylactic treatment infected full garden in 3 days not in time, cause the situation of total crop failure, gray mold is in the control of actual disease, be difficult to take effectively thoroughly to cut off source of infection, when pathogene invades, also be difficult to eliminate pathogen thoroughly, at present under the operation of reality plantation, control for gray mold generally takes fumigation and spraying medicine method two kinds to walk abreast, the medicine used is procymidone, tpn, iprodione, the agricultural chemicals such as the poly-phonetic mould amine of astatine are prevented and treated, although the situation of Pesticide use recited above can be prevented and treated, but its residual pathogene is under suitable environmental condition, breeding still can be continued after drug effect, therefore develop immunity to drugs after pathogene several generations, agricultural chemicals is used to carry out gray mold control, no matter adopt sootiness, the method sprayed, in reality plantation, its pathogene develops immunity to drugs to agricultural chemicals conventional on the market at present, in control more difficulty, and use these agricultural chemicals easily to produce residue of pesticide, land pollution, outside spoiled soil vigor, also the Quality Down of finished product fruit can be caused.
Present consumer payes attention to gradually for nuisanceless, green food, requirement for Detecting Pesticide standard is more and more higher, how a kind of control agent of gray mold of nontoxic, harmless boilogical type is provided, not only can suppress the growth of gray mold, trace element during can providing plant growth again supplements, except not developing immunity to drugs when disease control, the raw material adopted, easily by the organic-biological control agent that soil and plant absorption decompose, is a solution being badly in need of at present wanting on agricultural planting.
Summary of the invention
Technical problem to be solved by this invention is to provide with a kind of antibacterial protein peptide bond chelated nano complexing monovalence copper produced with vegetalitas lactic acid bacteria, forms the strong oxidizer destroying pathogen cells wall, and then the gray mold pathogene that deactivation has been infected, utilize vegetalitas lactic acid bacteria to the function of dissolving chitin, form chitin jelly, in order to the fruit of coated plant, stem, leaf, prevent the effect that pathogene invades, a breathable diaphragm is formed in the appearance of plant, prevent infecting of pathogene, advantage of the present invention not only can not produce pollution by environmental pollution, the composition used is the nutrient of demand of plant growth, easily be absorbed and used by plants, enter in soil and automatically degraded by the microorganism of soil, except being the control agent of the harmless a kind of gray mold of nature, not only play the incidence of disease preventing and treating gray mold, outside the infecting of isolated gray mold, only need in addition to adopt the method sprayed in control, do not need sootiness+spray dual work, reduce and preventing and treating the human input of gray mold, more can increase finished product fruit sugariness and mouthfeel, increase the quality of finished product fruit, the present invention is the biocontrol agent that not only can become economic worth, the otherwise address technical barrier of residue of pesticide and pathogene disease resistance.
The present invention solves the problems of the technologies described above the preparation method that the technical scheme adopted is a kind of chitin copper lactate gray mold control agent, its material mixture ratio and processing step as follows:
1, material:
(1), material I vegetalitas lactobacillus suspension 80%, be main material by sponge gourd tender leaf, fermentation culture is mixed by brown sugar 15%+ zinc sulphate 2%+ manganese sulphate 1.5%+ magnesium sulfate 2%;
(2), material II complexing nano monovalence copper 5%, material III chitin 15%;
2, processing step:
(1), collect sponge gourd tender leaf after, with the ratio of fermentation culture 2:1, put into fermentation cauldron, fermentation 7d, the temperature maintaining fermentation cauldron between yeast phase is between 30 DEG C-35 DEG C, and the rear filtration residue that fermented obtains suspension, is vegetalitas lactobacillus suspension;
(2) that, is blown slowly by material II puts in the vegetalitas lactobacillus suspension of completing steps 1, until zymotic fluid becomes dark green;
(3), further by material III, put into the solution after completing steps 2, after stirring, after leaving standstill 24h, solution becomes viscous fluid, is a kind of chitin copper lactate gray mold control agent;
Advantage of the present invention:
The present invention contrasts prior art following effect: the present invention is on a kind of application sponge gourd blade on every square centimeter, saccharomycete containing 10-15 ten thousand, lactic acid bacteria, culture fluid is utilized to turn out natural vegetalitas lactic acid bacteria and saccharomycete, produce antibacterial protein peptide, copper lactate is formed by antibacterial protein peptide bond chelated nano complexing monovalence copper, the function that chitin forms colloid substance can be dissolved by zymotic fluid, the chitin of powdery is formed colloid, when spraying on plant, not only play the cell wall destroying gray mold pathogene, block the sporulation of pathogene, in addition the fruit of plant, stem, leaf one-tenth one deck has the diaphragm of gas permeability, block the infection way of pathogene, and do not affect the effect of plant photosynthesis and breathing, the fruit plant is directly sprayed during use, stem, Ye Shang, play outside anti-two functions with controlling, element used is in addition the trace element needed for plant, not only can increase outside the nutritional supplementation of plant, can also by the microbial decomposition in soil, there is no the problem of residue of pesticide and soil pollution, the governance role of the present invention to pathogene is by external damage cell wall in addition, not utilize nerve or stomach toxicity sterilized principle for germ, so the pesticide resistance of pathogene can not be caused, it is a kind of product of worth large-scale promotion application, solve the problem that plantation at present relies on agricultural chemicals.
Accompanying drawing explanation
Fig. 1 is the process flow diagram of a kind of preparation method of chitin copper lactate gray mold control agent
Embodiment
1, case one: farm, Zhangjiagang, Suzhou City of Jiangsu Province, utilizes facility plastic greenhouse to plant edible-type tomato 100 mu of ground, the mid-April in this year, the fruit finding that there is the tomato of 30% has the mould layer of grey, through being judged as tomato gray mold; Apply chitin copper lactate gray mold control agent of the present invention 500 times of liquid to spray, within second day, find that mould layer presents and tighten dry shape, through sampling microscopic examination, discovery conidiophore reduces, through laboratory cultures, lose the bacterium colony of gray mold pathogene, after spray 1000 times of liquid, to the symptom having no gray mold when uprooting plants after their edible portions have been harvested every maintenance in 7 days;
2, case two: strawberry cultivating family, Dongshan Town, Suzhou City of Jiangsu Province, application simple plastic greenhouse plantation beauty strawberry 10 booths, in the February in this year, find when temperature raises that the strawberry in booth grows the mould layer of grey, through being judged as grey mould fruit rot of strawberry; Apply chitin copper lactate gray mold control agent of the present invention 500 times of liquid to spray, within second day, find that mould layer presents and tighten dry shape, 1000 times of liquid are sprayed every maintenance in 10 days backward, to the symptom having no gray mold at the end of strawberry at the beginning of 5 months, the sugariness of strawberry fruit is detected as 12-14 degree through sugariness detector, increase 3-4 degree than strawberry sugariness 9-10 originally, arround the strawberry sugariness of plantation family plantation at 7-8 degree;
3, case three: Zhangjiagang, Suzhou City of Jiangsu Province three Fengzhen, apply outdoor plantation summer black grape, when May, grape was born fruit, find that grape occurs white dot and with the situation of the rotten shape of yellow water, be judged as the omen infecting grape grey mould, apply chitin copper lactate gray mold control agent of the present invention 500 times of liquid to spray, within 3 days, check to have no afterwards and spread, sprayed 1000 times of liquid to prevent and treat as maintenance every 15 days, gray mold outbreak is had no when July gathers, its grape painted than arround fast 10 days of vineyard, the residue of pesticide of grape detect in local agriculture test department, do not detect residue of pesticide,
By above actual in the application in land for growing field crops, when between the gray mold high-incidence season, apply a kind of chitin copper lactate gray mold control agent of the present invention 500 times of liquid to spray, treatment infected plant has served the effect of the activity suppressing germ, and spray the protective liquid of low solubility at a fixed time, also can play outside preventive effect, separately can increase colourity and the sugariness of fruit, increasing quality and the price of commodity fruit, is the solution of a kind of green non-poisonous gray mold control be worthy to be popularized.
Although the present invention discloses as above with preferred embodiment; so itself and be not used to limit the present invention, any those skilled in the art, without departing from the spirit and scope of the present invention; when doing a little amendment and perfect, therefore protection scope of the present invention is when being as the criterion of defining with claims.
Claims (2)
1. the preparation method of a chitin copper lactate gray mold control agent, it is characterized in that by vegetalitas lactobacillus suspension 80%, complexing nano monovalence copper 5%, chitin 15%, through the Biological Control of Gray Mold agent of a kind of biotype nontoxic residue-free that fermentation is made with the technique of chelating.
2. the preparation method of a kind of chitin copper lactate gray mold control agent as claimed in claim 1, adopts following processing step to make:
1), collect the tender leaf of sponge gourd, with the mixed liquor of brown sugar 15%+ zinc sulphate 2%+ manganese sulphate 1.5%+ magnesium sulfate 2%, carry out fermentation 7d with the ratio of 2:1, the rear filtration residue that fermented obtains suspension, is vegetalitas lactobacillus suspension;
2), further by complexing nano monovalence copper liquid, that blows slowly pours in vegetalitas lactobacillus suspension, until the color of bacterium liquid becomes dark green;
3), further by chitin put in the solution after completing steps 2, after stirring, after leaving standstill 24h, until liquid become thick after namely obtain a kind of chitin copper lactate gray mold control agent.
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| CN201510643437.1A CN105123765A (en) | 2015-10-08 | 2015-10-08 | Preparing method for chitin copper-lactate gray-mold preventing and treating preparation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114287282A (en) * | 2021-12-14 | 2022-04-08 | 山东省林业科学研究院 | Method for increasing yield by combining pruning of branch ends of figs with antibacterial film |
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| CN1076829A (en) * | 1992-04-03 | 1993-10-06 | 格里菲公司 | Copper complex bactericides agent/fungicide and manufacture method thereof |
| CN1125040A (en) * | 1994-12-19 | 1996-06-26 | 胡瑞省 | A kind of fruit tree fungicide and preparation method thereof |
| CN1279019A (en) * | 1999-06-25 | 2001-01-10 | 中国科学院大连化学物理研究所 | Plant ecological regulator |
| CN101225000A (en) * | 2008-01-25 | 2008-07-23 | 叶水源 | Growth regulator promoting healthy growth of animal plant and preparation method thereof |
| CN101690735A (en) * | 2009-09-16 | 2010-04-07 | 周汉华 | Complex microorganism health-protection agent and preparation method thereof |
| CN104322595A (en) * | 2014-10-22 | 2015-02-04 | 苏州科大微龙信息技术有限公司 | Mint-copper false smut preventing-treating agent and preparation method thereof |
-
2015
- 2015-10-08 CN CN201510643437.1A patent/CN105123765A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1076829A (en) * | 1992-04-03 | 1993-10-06 | 格里菲公司 | Copper complex bactericides agent/fungicide and manufacture method thereof |
| CN1125040A (en) * | 1994-12-19 | 1996-06-26 | 胡瑞省 | A kind of fruit tree fungicide and preparation method thereof |
| CN1279019A (en) * | 1999-06-25 | 2001-01-10 | 中国科学院大连化学物理研究所 | Plant ecological regulator |
| CN101225000A (en) * | 2008-01-25 | 2008-07-23 | 叶水源 | Growth regulator promoting healthy growth of animal plant and preparation method thereof |
| CN101690735A (en) * | 2009-09-16 | 2010-04-07 | 周汉华 | Complex microorganism health-protection agent and preparation method thereof |
| CN104322595A (en) * | 2014-10-22 | 2015-02-04 | 苏州科大微龙信息技术有限公司 | Mint-copper false smut preventing-treating agent and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114287282A (en) * | 2021-12-14 | 2022-04-08 | 山东省林业科学研究院 | Method for increasing yield by combining pruning of branch ends of figs with antibacterial film |
| CN114287282B (en) * | 2021-12-14 | 2023-01-24 | 山东省林业科学研究院 | A method of pruning fig branches and using antibacterial film to improve yield |
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Application publication date: 20151209 |