CN105233302B - 一种流感血凝素糖蛋白多聚物纳米颗粒的制备方法 - Google Patents

一种流感血凝素糖蛋白多聚物纳米颗粒的制备方法 Download PDF

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CN105233302B
CN105233302B CN201410328217.5A CN201410328217A CN105233302B CN 105233302 B CN105233302 B CN 105233302B CN 201410328217 A CN201410328217 A CN 201410328217A CN 105233302 B CN105233302 B CN 105233302B
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influenza virus
hemagglutinin
terminal
influenza
gene
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Chinese (zh)
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CN105233302A (zh
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吴军
刘波
唱韶红
巩新
王莎
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Institute of Bioengineering Chinese Academy of Military Medical Sciences
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Institute of Bioengineering Chinese Academy of Military Medical Sciences
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Priority to PCT/CN2015/088748 priority patent/WO2016004904A2/fr
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/11—Orthomyxoviridae, e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Pulmonology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Communicable Diseases (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201410328217.5A 2014-07-10 2014-07-10 一种流感血凝素糖蛋白多聚物纳米颗粒的制备方法 Active CN105233302B (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN201410328217.5A CN105233302B (zh) 2014-07-10 2014-07-10 一种流感血凝素糖蛋白多聚物纳米颗粒的制备方法
PCT/CN2015/088748 WO2016004904A2 (fr) 2014-07-10 2015-09-01 Procédé de préparation de nanoparticules polymères de glycoprotéines correspondant à l'hémagglutinine de la grippe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410328217.5A CN105233302B (zh) 2014-07-10 2014-07-10 一种流感血凝素糖蛋白多聚物纳米颗粒的制备方法

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CN105233302A CN105233302A (zh) 2016-01-13
CN105233302B true CN105233302B (zh) 2018-10-19

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CN (1) CN105233302B (fr)
WO (1) WO2016004904A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106868025B (zh) * 2017-03-13 2020-01-21 中国人民解放军军事医学科学院生物工程研究所 用酵母制备三聚体埃博拉病毒糖蛋白突变体的方法
CN107034225B (zh) * 2017-05-04 2020-01-17 中国人民解放军军事医学科学院生物工程研究所 制备埃博拉病毒糖蛋白与基质蛋白融合突变体的方法
IT202100024620A1 (it) 2021-09-27 2023-03-27 Graficonsul S R L Mattonella componibile per esposizione di prodotti pubblicitari

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101405026A (zh) * 2006-02-02 2009-04-08 全球免疫股份有限公司 用于诱导免疫应答的基于酵母的疫苗
CN103209990A (zh) * 2010-10-30 2013-07-17 刘大才 重组流感病毒血凝素（ha）抗原蛋白和含其的疫苗

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE039100T2 (hu) * 2009-06-24 2018-12-28 Medicago Inc Hemagglutinint tartalmazó kimer influenzavírus-jellegû részecskék
CN104711278A (zh) * 2013-12-16 2015-06-17 特菲(天津)生物医药科技有限公司 含h7n9病毒ha基因的重组序列、重组杆状病毒及该病毒在疫苗制备中的应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101405026A (zh) * 2006-02-02 2009-04-08 全球免疫股份有限公司 用于诱导免疫应答的基于酵母的疫苗
CN103209990A (zh) * 2010-10-30 2013-07-17 刘大才 重组流感病毒血凝素（ha）抗原蛋白和含其的疫苗

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralizing antibodies in rabbits and mice;TN Athmaram et.al.;《Virology Journal》;20111231;第524卷(第8期);摘要、第1页右栏最后1段、第3页右栏第1段、第9-10页、Figure 1 *
甲型H1N1流感病毒血凝素HAl在糖基工程毕赤酵母中的表达;张晓红等;《生物技术通讯》;20120131;第23卷(第1期);第1-5页 *

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WO2016004904A3 (fr) 2016-03-17
CN105233302A (zh) 2016-01-13
WO2016004904A2 (fr) 2016-01-14

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