CN1057517C

CN1057517C - Method for production of L-arginine

Info

Publication number: CN1057517C
Application number: CN96100529A
Authority: CN
Inventors: 孙复兴
Original assignee: Individual
Current assignee: Individual
Priority date: 1996-04-08
Filing date: 1996-04-08
Publication date: 2000-10-18
Anticipated expiration: 2016-04-08
Also published as: CN1161959A

Abstract

本发明公开了一种用生产胱氨酸的废液或蛋白质水解液提取L-精氨酸的新方法。它是将原料液浓缩、过滤后经两次碱中和和两次精制即得到L-精氨酸成品。该方法的产品回收率可达90%，原料易得，工艺流程短，条件容易控制，生产中所得付产品工业级氨化铵、复合氨基酸粉也有很好的市场前景，使L-精氨酸的生产成本大为降低，而且无污染问题，因此，本发明具有明显的经济效益和社会效益。The invention discloses a new method for extracting L-arginine from cystine-producing waste liquid or protein hydrolyzate. It is obtained by concentrating and filtering the raw material liquid, and then undergoing two times of alkali neutralization and two times of refining to obtain the finished product of L-arginine. The product recovery rate of this method can reach 90%, the raw material is easy to get, the technical process is short, and the conditions are easy to control, and the by-products industrial grade ammonium ammoniation and compound amino acid powder obtained in the production also have good market prospects, making L-arginine The production cost is greatly reduced, and there is no pollution problem, therefore, the present invention has obvious economic benefit and social benefit.

Description

The arginic method of a kind of production L-

The invention discloses a kind of waste liquid or protein hydrolyzate of producing Gelucystine of utilizing and produce the arginic method of L-, it belongs to biochemical field, also belongs to pharmaceutical sanitary field.

The L-arginine is a preparation amino acid infusion solutions requisite bulk drug, also is a kind of active drug for the treatment of hepatic coma, in addition, also has been widely used aspect many at food, makeup, feed etc.Have only these series products of several manufacturer production such as Japan, West Germany at present in the world, China begins to have aspect medical from the seventies, and mainly be from Japanese import amino acid infusion solutions finished medicines, or L-arginine bulk drug, the arginic price of nineteen ninety-five every kilogram of L-is more than 20 dollar.Produce both at home and abroad at present method that the L-arginine adopted three kinds of fermentation method, resin isolation method and precipitation agent extraction methods are arranged.When using fermentative production, owing to can not there be other assorted bacterium to exist, it is very strict that range request is crossed in production operation, also will further separate after the fermentation, and the cycle of production technique is long, and the water power consumption is big, and production cost is also very high.Adopt the resin isolation method production arginic cycle of L-also very long, and also need a large amount of soda acids come regenerating resin after a production cycle, turnout is little, and the purity of product is not high yet, and therefore, the production cost of this method is also very high.And the disadvantage of precipitation extraction method is that the precipitation agent that is adopted all is an organic compound, and as o-Xylol, phenyl aldehyde etc., not only price is expensive for this class precipitation agent, and volatile, and easy firing reclaims not exclusively, therefore, causes the injury of human body and the pollution of environment.

Although above-mentioned processing method exists many shortcoming and defect, owing to also do not search out better production method so far, therefore, each manufacturer still adopts aforesaid method to produce the L-arginine always.Purpose of the present invention: produce the arginic deficiency of L-in order to overcome above-mentioned fermentation method, resin isolation method and precipitation agent extraction method, a kind of novel method is provided, and this method should be with short production cycle, simple to operate, production cost is lower, and does not cause problems such as environmental pollution.

Be the technical measures that realize that purpose of the present invention adopts:

Utilize the different amino acid whose iso-electric point values and the difference of dissolubility property, select suitable pH value and temperature condition to reach that L-is arginic to be separated and the purified purpose, its technical process is as follows:

The technical process explanation:

1) will produce waste liquid behind the Gelucystine or protein hydrolyzate contain the arginic liquid concentration of L-to degree Beaume about 24～26 degree, cooling, filter, remove and desalt and other impurity.

2) be 6～8 with filtrate with the alkali pH value that neutralizes, cool to about 10 ℃, remove by filter the mixture of neutral amino acids.

3) the filtrate reconcentration is about 28 degree to degree Beaume, adds isopyknic ethanol, stirs and is warmed up to about 45 ℃, with the alkali pH value that neutralizes is 10～12, have the crystallization of a large amount of L-arginine to separate out this moment, deposits at low temperatures more than 12 hours, filters and promptly get L-arginine crude product.

4) crude product is added small amount of ethanol and wash, filter, dissolve with water saturation under 60 ℃, add activated carbon decolorizing, filter, filtrate is concentrated into hypersaturated state, cooling, and stirred crystallization gets work in-process at low temperatures.

5) work in-process are warmed to 60 ℃ of saturated dissolvings down, add activated carbon decolorizing, filter rear filtrate and be concentrated to hypersaturated state, cooling, stirred crystallization at low temperatures.Isolate crystallisate and, dry and promptly get L-arginine finished product with 75% washing with alcohol 2～4 times.

Compared with prior art, the beneficial effect that adopts method of the present invention to reach: compared with prior art, the principle that this method is adopted has difference in essence, and the beneficial effect that is reached mainly shows the following aspects:

1) this processing method mainly is pH value and the temperature condition in the control production process, makes actually operating more simple and convenient than prior art, separate easily, the reproduction height of technology.

2) without precipitation agent, resin isolation, be raw material, make that the environmental problem in the production process is solved and have only with soda acid.

3) when producing L-arginine product, the chlorine that also obtains technical grade divides ammonia and compound amino acid powder by product, and these by product purposes prospects are also fine.

4) production cycle shortens greatly, just can obtain L-arginine product in 1～2 day, and the rate of recovery of product reaches 90%.

Because above-mentioned unusual effect also makes the arginic production cost of L-be reduced to every kilogram and only needs 60 yuan of Renminbi, and also can further produce other amino acid with this method, as L-Xie Ansuan, L-phenylalanine, L-Serine, L-Histidine etc.

The present invention is further illustrated below in conjunction with specific embodiment: embodiment:

1) get 100 liters and produce primary mother liquor behind the Gelucystines, be concentrated to 2/3 of original volume, cooling is filtered, 30 kilograms of salt and other impurity, 40 liters of filtrates;

2) 40 liters of this filtrates being added the alkali PH that neutralizes is 6～8, cools to 10 ℃ of after-filtration, 15 kilograms of neutral amino acidss, keep filtrate:

3) with in the alkali and above-mentioned filtrate be 6～8 to pH value, add 30 liters of ethanol, stir, be 11 with the alkali pH value that neutralizes again, a large amount of L-arginine crystallizations is arranged this moment, low-temp storage is more than 12 hours, filtration obtains L-arginine crude product.

4) crude product is washed with small amount of ethanol, filter 4.5 kilograms of crude products, 60 ℃ of following saturated being dissolved in the water, and add 5% gac of crude product weight, stirred 30 minutes, filter, filtrate concentrates, and filtration is desalted, reconcentration is to hypersaturated state, cooling, stirred crystallization at low temperatures, filter 2.5 kilograms of work in-process;

5) with work in-process saturated dissolvings under 60 ℃, add the small amount of activated decolouring, filter twice, the filtrate reconcentration is to hypersaturated state, add isopyknic ethanol under the low temperature, stirred crystallization is filtered the post crystallization product with 75% washing with alcohol 2～3 times, oven dry promptly gets 2 kilograms of L-arginine finished products, the rate of recovery 90%.

Claims

1. one kind is that feedstock production is produced the arginic method of L-with waste liquid behind the production Gelucystine or protein hydrolyzate, it is characterized in that: stock liquid is concentrated to degree Beaume 24～26 degree, cooling, filter, filtrate is with alkali PH6～8 that neutralize, cool to 10 ℃, filter, the filtrate reconcentration adds isopyknic ethanol to degree Beaume 28 degree, stirs and is warmed up to 45 ℃, with alkali PH10～12 that neutralize, stand at low temperature more than 12 hours crystallization promptly get L-arginine crude product, again through twice refining, washing, oven dry promptly get L-arginine finished product.

2. by the described method of claim 1, it is characterized in that: when crude product refining, crude product 60 ℃ of saturated dissolvings down, is added the activated carbon decolorizing after-filtration, reconcentration is to hypersaturated state, crystallisation by cooling; Through twice refining after product with 75% washing with alcohol 2～4 times.