CN106689114B - Method for making unhaired vertebrate animal specimen and used injector - Google Patents
Method for making unhaired vertebrate animal specimen and used injector Download PDFInfo
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- CN106689114B CN106689114B CN201710007517.7A CN201710007517A CN106689114B CN 106689114 B CN106689114 B CN 106689114B CN 201710007517 A CN201710007517 A CN 201710007517A CN 106689114 B CN106689114 B CN 106689114B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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Abstract
The invention belongs to the technical field of animal specimen preparation, and particularly relates to a method for preparing a stripped animal specimen of a non-fur vertebrate and an injector used in the method. The invention provides a method for manufacturing a special stripped specimen for a non-fur vertebrate, which aims at the basic characteristics of the non-fur vertebrate, is based on the special advantages of an alpha cyanoacrylate compound and utilizes a specially designed alpha cyanoacrylate compound injector. The front end of the needle head is completely sealed, the inner wall of the inner cavity of the needle head is completely embedded by a teflon material, and the injection holes are uniformly distributed on the outer wall of the front end of the needle head, so that the alpha cyanoacrylate compounds are prevented from being adhered to the wall of the inner cavity of the needle head to generate polymerization reaction to block an injection channel, no residue is left, the number of pinholes of the injector on the body surface of an animal specimen is greatly reduced, the problem of uneven filling is effectively solved, and the manufacturing method of the alpha cyanoacrylate compounds in the non-quilt vertebrate is perfected.
Description
Technical Field
The invention belongs to the technical field of animal specimen preparation, and particularly relates to a method for preparing a stripped animal specimen of a non-fur vertebrate and an injector used in the method.
Background
Animal specimens are the first material of biological science research and the best material for practicing practice and teaching. Animal specimens are the best tool for students to develop viewing abilities, find problems, and gain new knowledge. With the continuous development of the times and the continuous progress of economy, the application range of the animal specimen is not limited in scientific research and teaching, but is used as a decoration of ornaments and companies and enterprises, and even a mode that pet breeders think of dead pets by accident, so that the animal specimen enters the lives of people. However, most of the existing methods for preparing animal specimens adopt toxic substances such as formalin, phenol, arsenic and the like for corrosion prevention, and the agents can cause certain damage to specimen makers and specimen users, and the preparation methods are complex, the technical requirements on the operation process are high, the preparation cost is high, and the application range and the practical value of the specimens are limited.
The alpha cyanoacrylate compound has the advantages of wide source, low cost, simple operation, no toxicity and the like on the upper surface of animal specimen preparation, however, because the reaction speed of the alpha cyanoacrylate compound is too fast and is not convenient to control, the difficulty of using the alpha cyanoacrylate compound to prepare the actuating specimen is increased, particularly in the process of preparing reptiles, amphibians and cartilaginous fishes, because the body surface of the animals is not protected by fur and hard scales and the morphological structure of most animals is more complex, so the difficulty and the cost of manufacturing the stripped specimen of the animal are often increased due to the problems of too complicated prosthesis manufacturing and the like in the manufacturing process, and the success rate of making animal specimens is greatly reduced due to the over-high reaction speed and uneven reaction of the alpha cyanoacrylate compounds, so that the quality of making the animal specimens is reduced and the finished products are beautiful. For example, in the process of making shark specimens in cartilaginous fish, the muscle tissue of the shark is very dense, which is very unfavorable for the penetration and sealing of alpha-cyanoacrylate compounds, even the alpha-cyanoacrylate compounds used for sealing can only adhere to the body surface of the shark specimen. Although the alpha cyanoacrylate compounds can be effectively guided to permeate into muscle tissues of sharks by using a cotton wool filling mode, the sharks belong to cartilaginous fishes, abdominal cavities and ribs which are formed in an evolution mode are not provided, only a spine from top to bottom is provided, the specimen prepared by using the alpha cyanoacrylate compounds can be sealed and preserved after the sharks are completely dehydrated, and the whole shark specimen prepared by using the cotton wool filling mode can be seriously deformed and distorted, so that the due streamline fish body of the sharks is completely lost, and the ornamental value and the market value are lost. The special defects and shortcomings of the animal specimen prepared by using the alpha cyanoacrylate compound not only exist in the preparation process of the stripped animal specimen of the cartilage fish such as sharks, but also exist in the preparation process of amphibians with incomplete skeletal systems and more complex morphological structures and stripped animal specimens of reptiles with special morphological structures such as snakes and turtles.
Although various types of injectors for alpha cyanoacrylate compounds already exist in the market, the injectors for alpha cyanoacrylate compounds in the market can only be used for smearing the surface of an object and are not suitable for internal injection and preparation of various animal specimens, particularly deliquescence specimens of unhaired vertebrates due to different application fields. The problems of distortion and deformation of finished products of samples caused by nonuniform filling, excessively high reaction speed and nonuniform reaction of the alpha cyanoacrylate compound restrict the use and development of the alpha cyanoacrylate compound in sample preparation, and the improvement and innovation of the alpha cyanoacrylate compound are imperative.
Disclosure of Invention
In order to solve the above problems, the present invention provides a method for producing a depilatory vertebrate animal specimen and an injector used in the method, which not only effectively overcomes the disadvantages and shortcomings of using alpha cyanoacrylate compounds in depilatory vertebrate animal specimens, but also greatly reduces the cost of producing traditional depilatory vertebrate animal specimens, and improves the quality and appearance of the finished product of depilatory vertebrate animal specimens.
In order to achieve the purpose, the invention adopts the technical scheme that:
a cyanoacrylate compound injector for preparing animal specimen is composed of a cylinder with a piston end extended into the internal cavity of cylinder, a piston-core rod with a hollow structure with one open end and one sealed end, and a needle head with a cavity communicated with said internal cavity of cylinder.
The injection holes are symmetrically arranged in 2.
The injection holes are arranged at two sides of the front end of the inner cavity of the needle head.
The front end of the sealing end of the needle head is arranged in a pointed shape.
The front end of the needle cylinder is provided with a connecting pipe communicated with the negative pressure type sliding stroke cavity, the opening end of the needle head is provided with a joint, the connecting pipe is inserted in the joint to form a detachable connecting structure, and the negative pressure type sliding stroke cavity is communicated with the injection hole sequentially through the inner cavity of the connecting pipe, the inner cavity of the joint and the inner cavity of the needle head to form a flow injection channel of cyanoacrylate compounds.
The rear end of the piston core rod is provided with a handle.
The length of the needle head is 5-25 cm.
A method of making a depilatory vertebrate animal preparation using a syringe, comprising the steps of:
(1) treating dead bodies of the naturally occurring unhaired vertebrates with absolute alcohol;
(2) dissecting the remains of the processed non-fur vertebrate, and removing the muscle, skeleton and organs of the animal, wherein the rest parts are the external parts of the animal;
(3) fixing and filling the animal external body part in a bracket fixing and absorbent cotton filling mode, and then sewing the cut of the filled animal external body part to obtain an animal specimen precursor;
(4) extracting the alpha cyanoacrylate compound by using a cyanoacrylate compound injector, inserting a needle head of the injector into the animal specimen precursor, and injecting the alpha cyanoacrylate compound;
(5) and completely drying the alpha cyanoacrylate compound to be injected.
And (4) injecting the alpha cyanoacrylate compound to other parts of the animal specimen precursor.
The alpha cyanoacrylate compounds suitable for making animal specimen are alpha ethyl cyanoacrylate, alpha octyl cyanoacrylate and alpha butyl cyanoacrylate, and the injector of the present invention may be also used in injecting various alpha cyanoacrylate compounds.
The invention has the beneficial effects that:
1. the front end of the syringe needle of the syringe is completely sealed, and the inner wall of the inner cavity of the needle is completely embedded by using a teflon material, so that the alpha cyanoacrylate compounds are prevented from being adhered to the wall of the inner cavity of the needle to generate polymerization reaction and block an injection channel; the injection holes are uniformly distributed on the outer wall of the front end of the needle head, so that the alpha cyanoacrylate compound can be effectively ensured to fully permeate into fillers and muscle tissues in an animal body without residues, the number of pinholes of the injector on the body surface of an animal specimen is greatly reduced, the problem of uneven filling is effectively solved, the manufacturing method of the alpha cyanoacrylate compound in the non-fur vertebrate is perfected, and the range of the alpha cyanoacrylate compound in the manufacturing of the animal specimen is expanded.
2. Through the process of long biological evolution and mutual selection, different animals are evolved into different morphological structures to form different tissue characteristics. The invention provides a method for manufacturing a special stripped specimen for a non-fur vertebrate, which aims at the basic characteristics of the non-fur vertebrate, is based on the special advantages of an alpha cyanoacrylate compound and utilizes a specially designed alpha cyanoacrylate compound injector.
3. The invention firstly adopts absolute ethyl alcohol to treat the skin of an animal to be prepared, so that the animal skin is completely dehydrated, oil which prevents alpha cyanoacrylate compounds from permeating into the body is dissolved in the absolute ethyl alcohol, and the treatment of the absolute ethyl alcohol can also shape and solidify the skin of the animal such as Chinese water snakes and black fin shark. The invention does not reserve the muscle tissue and the bone tissue of the unhaired animal, and does not combine the prosthesis and the peeled skin after the prosthesis is manufactured, but directly uses the iron wire bracket, then fills the bracket with the absorbent cotton, and finally seals, prevents corrosion and cures from the inside by using the injector to form a firm supporting material.
4. The bracket and filling mode has wide application in the traditional process of peeling the specimen by birds, but the method is not suitable for manufacturing the unhaired fur-animal peeled specimen because the filling material is soft and fluffy absorbent cotton. After the alpha cyanoacrylate compound is injected into the abdominal cavity filled with the hairless vertebrate support through the injector, the alpha cyanoacrylate compound is subjected to polymerization reaction in the middle of the fluffy absorbent cotton to form a firm poly alpha cyanoacrylate compound, and the alpha cyanoacrylate compound is not subjected to large deformation after the reaction with the absorbent cotton, so that the method effectively overcomes the obvious defects and shortcomings in appearance and texture in the process of manufacturing the hairless vertebrate specimen.
5. The invention is neither an extension of the traditional animal specimen preparation method nor the improvement and the defect of simply using the alpha cyanoacrylate compound to prepare the animal specimen. Compared with the traditional animal specimen support and filling method, the invention has incomparable advantages in appearance and texture compared with the finished product made of the non-fur vertebrate specimen.
6. Compared with the animal specimen prepared on the basis of the advantages of the pure alpha cyanoacrylate compound, the preparation method has the following advantages in the preparation range: the invention is mainly suitable for manufacturing the depilated specimen of the non-fur vertebrate, the latter is more suitable for invertebrates, fur vertebrates and part of teleosts, and has obvious disadvantages compared with the manufacturing of the depilated specimen of the non-fur vertebrate with smooth body surface and complex structure. In the aspect of preservation concept: the invention eliminates almost all muscles, bones and various tissues and organs of the animal body, is more suitable for consumers and commercialization in the market, does not pay attention to objective physiological characteristics of the animal body, and the latter is more suitable for preserving the muscle structure and tissue physiological characteristics of the animal as much as possible, so that the product is more suitable for scientific research and biological teaching. In the manufacturing method, the invention mainly adopts the manufacturing processes of dehydration, stripping, support, filling, corrosion prevention and plasticity, and the latter adopts the manufacturing processes of planning release, soaking, filling and corrosion prevention. Although the method increases the operation steps compared with the former method, the material cost is not increased, and the technical difficulty is reduced. Moreover, compared with the latter method, the method of the invention can meet the needs of consumers, and is more beneficial to the development and application of the animal decortication marketization.
Drawings
Fig. 1 is a schematic view (partially cut away) of the structure of the syringe of the present invention.
Fig. 2 is a cross-sectional view of the needle of the syringe of the present invention.
FIG. 3 is an enlarged view of a portion of the needle of FIG. 1 according to the present invention.
Detailed Description
The following examples further illustrate the embodiments of the present invention in detail.
Example 1
As shown in figures 1-3, the injector of the invention comprises a needle cylinder 1, a piston core rod 2 and a needle head 5, wherein a piston end 2a of the piston core rod 2 extends into the inner cavity of the needle cylinder from the opening part at the rear end of the needle cylinder 1, the needle head 5 is arranged at the front end of the needle cylinder 1, the inner cavity of the needle cylinder between the needle head 5 and the piston end of the piston core rod forms a negative pressure type sliding stroke cavity 1a, the piston core rod slides forwards and backwards along the length direction of the inner cavity of the needle cylinder to form a length adjusting structure of the negative pressure type sliding stroke cavity, the needle head 5 is a hollow structure with an opening at one end and a sealed at one end, the opening end of the needle head is connected with the negative pressure type sliding stroke cavity 1a, the needle head inner cavity 53 of the needle head is communicated with the negative pressure type sliding stroke cavity 1a, injection holes 6 communicated with the needle head inner cavity 53 are evenly distributed on the outer wall of the needle head 5 along the circumferential direction, and an anti-sticking layer 52 made of Teflon material covers the inner wall of the needle head inner cavity 53.
In order to ensure the using effect, the injection holes 6 are symmetrically arranged in 2.
The injection holes 6 are arranged at two sides of the front end of the needle head cavity 53, namely the outer edge of the front end of the injection hole is flush with the bottom surface of the needle head cavity, and the injection hole is tightly connected with the rear part of the sealing end, so that the alpha cyanoacrylate compound can not be remained in the needle head cavity in the using process.
The front end of the sealing end 51 of the needle 5 is arranged in a sharp manner.
The front end of the needle cylinder 1 is provided with a connecting pipe 3 communicated with a negative pressure type sliding stroke cavity 1a, the opening end of the needle head 5 is provided with a joint 4, the connecting pipe is inserted in the joint to form a detachable connecting structure, and the negative pressure type sliding stroke cavity 1a is communicated with the injection hole sequentially through the inner cavity of the connecting pipe, the inner cavity of the joint and the inner cavity of the needle head to form a flow injection channel of the alpha cyanoacrylate compound. The connecting pipe is of a columnar tubular structure, the inner cavity of the joint 4 is in a circular truncated cone shape, the connecting pipe is inserted into the circular truncated cone-shaped inner cavity of the joint and is connected with the circular truncated cone-shaped inner cavity of the joint in a size matching mode, and the detachable connection relation is conventional technology in the field.
The rear end of the piston core rod 2 is provided with a handle 2 b; the length of the needle head 5 is 5-25 cm.
The thickness of the anti-adhesion layer is designed into different thicknesses according to the size of a needle head of the syringe, and is generally 0.5-1 mm.
When the injector of the invention is used, the piston core rod is pushed, the length of the negative pressure type sliding stroke cavity is compressed, then the needle head is extended into the alpha cyanoacrylate compound container, the piston core rod is pulled outwards, the length of the negative pressure type sliding stroke cavity is gradually increased, under the action of negative pressure, the alpha cyanoacrylate compound enters the flow injection channel from the injection hole, the negative pressure type sliding stroke cavity is filled, then the needle head is taken out, the needle head is inserted into the interior of the animal specimen precursor, then the needle head is drawn outwards, the piston core rod is pushed, under the action of pressure, the alpha cyanoacrylate compound is sprayed out from the injection hole through the flow injection channel, the alpha cyanoacrylate compound is injected into the interior and the tissue structure of the animal specimen precursor while the needle head is drawn outwards, because the front end of the needle head is completely sealed, the inner wall of the inner cavity of the needle head is completely embedded by using the Teflon material, the alpha cyanoacrylate compounds are prevented from being adhered to the inner cavity wall of the needle head to generate polymerization reaction so as to block an injection channel; the injection holes are uniformly distributed on the outer wall of the front end of the needle head, so that the alpha cyanoacrylate compound can be effectively ensured to fully permeate into fillers and muscle tissues in an animal body without residues, the number of pinholes of the injector on the body surface of an animal specimen is greatly reduced, the problem of uneven filling is effectively solved, the manufacturing method of the alpha cyanoacrylate compound in the non-fur vertebrate is perfected, and the range of the alpha cyanoacrylate compound in the manufacturing of the animal specimen is expanded. The invention is far from only applied to the preparation of the stripped specimen of the non-fur vertebrate, can also be applied to the filling of cranial cavity, the preparation of muscle tissue specimen and the like in the preparation process of the stripped specimen of the mammal, and even can be applied to the field of non-specimen preparation.
EXAMPLE 2 preparation of peeled specimen of Chinese Water Snake
Animal materials: chinese water snake (about 5m long)
The experimental steps are as follows:
1. soaking the dead Chinese water snake in absolute ethyl alcohol for 48 h.
2. Taking out Chinese water snake remains soaked in absolute ethyl alcohol, placing the Chinese water snake bodies in an anatomical disk with the abdomen facing upwards, and cutting a cut (about 3 cm) longitudinally at 2/3 (namely 1m away from the head) of the Chinese water snake bodies by using an anatomical scissors. The skin and muscle at the incision are peeled off with forceps until the muscle and skin at the incision are completely separated (without damaging the skin of the Chinese water snake).
3. Cutting the snake body at the incision with a scalpel, peeling the snake body upwards from the incision to the head of the Chinese water snake with a pair of tweezers, keeping the snake head, and cutting the snake body (including muscles, organs and bones) below the head; peeling from the cut part downwards to 1cm position of the tail part of the Chinese water snake, reserving the tail part, and cutting the snake body (including muscle, organ and bone) above the tail part (reserving the head and tail of the snake as the external body part of the animal).
4. The remained Chinese water snakes are turned over again along the head and the tail by blunt iron wires with the length equivalent to that of the Chinese water snakes.
5. An iron wire (the diameter is about 1.6mm) which is about 5cm longer than the Chinese water snake is cut off by an iron wire clamp, the front end of the iron wire is ground into a blunt end, and the iron wire is inserted into the tail part of the Chinese water snake with stripped muscle along the head part of the Chinese water snake.
6. Filling Chinese water snake from the fracture between the head and the tail of the Chinese water snake with absorbent cotton, and sewing the fracture (the position is at the abdominal incision) of the Chinese water snake with a sewing needle after the filling is finished; after the suture is completed, the Chinese water snake is arranged into a required shape according to the requirement, and the redundant iron wire (as the precursor of the animal specimen) is cut off by using a wire clamp.
7. Extracting alpha ethyl cyanoacrylate with cyanoacrylate compound injector, inserting the needle of the injector into the sewed Chinese hydrophis abdominal cavity, injecting alpha ethyl cyanoacrylate into the Chinese hydrophis abdominal cavity while withdrawing the needle, and installing artificial snake eye after the alpha ethyl cyanoacrylate is completely dried.
The whole process of specimen preparation, the anhydrous state is guaranteed as far as possible.
The snakes are streamlined and are insufficient, the length and the thickness of the snakes are greatly different due to species difference, and a large incision is required to be cut in the manufacturing process if the internal organs are to be removed. As with most reptiles, the scale covering the surface of the snake often requires that the surface of the snake penetrate a certain number of cuts or pinholes in order for the alpha cyanoacrylate compound to penetrate the muscle tissue of the snake sufficiently and effectively. However, this greatly affects the aesthetic appearance of snake specimens. However, the most common polyurethane foaming agent prosthesis technology and animal specimen plasticizing technology often cost more than thousands of yuan, even ten thousands of yuan, far exceeding the value of the animal specimen. Therefore, most of the snake specimens which are most commonly used in the existing market are soaked specimens, and although the cost is low, the snake specimens only have certain scientific research value and cannot meet the requirements of market consumers, so the snake specimens have low market value and cannot be popularized in the market.
The invention combines the excellent properties of the bracket, the filling and the alpha cyanoacrylate compound, not only greatly reduces the cost of the polyurethane foaming agent and the animal specimen plasticizing technology, solves the problem of shriveling and deformation in the snake peeling specimen manufacturing process, but also adopts the method of the internal injection, sealing and corrosion prevention of the alpha cyanoacrylate compound, and avoids the problem of the appearance damage of the specimen caused by uneven reaction due to the fact that the reaction speed of the alpha cyanoacrylate compound is too high and is not easy to control.
EXAMPLE 3 preparation of peeled Barbie specimen
Animal materials: brazilian terrapin (about 15cm long)
The experimental steps are as follows:
1. treating the naturally dead corpses of the Brazilian terrapin: stripping the abdominal bone plate of the Brazilian terrapin from one side of the abdominal bone plate of the Brazilian terrapin to completely separate muscles, skin and bones from the abdominal bone plate; the tortoise shell of the Brazilian terrapin is opened from one side by using wire pliers, and the integrity of the tortoise shell and the tidiness of the cut edge are ensured when the tortoise shell is opened.
2. The viscera of the brazil terrapin after the tortoise shell is opened are removed, muscles and skeletons of the limbs, the head and the tail of the brazil terrapin are removed by using a depalletizing scissors and an ophthalmic forceps (the forelimb is removed to the carpal bone, the hindlimb is removed to the tarsal bone, and the head is removed to the skull, the eyeball of the brazil terrapin is removed by using the ophthalmic scissors, and the brain pulp of the brazil terrapin is removed by using a common syringe) (all the parts are kept as the external body part of the animal).
3. Placing Brazilian terrapin (animal external body part) in anhydrous ethanol, and replacing anhydrous ethanol until the anhydrous ethanol turns yellow until the color of the anhydrous ethanol is not changed within 12 h.
4. Taking out the Brazilian terrapin treated by the absolute ethyl alcohol, cutting iron wires (the diameter is about 1.6mm) which are about 3cm longer than the straight line from the head to the tail, the diagonal line from the left upper limb to the right lower limb and the diagonal line from the left lower limb to the right upper limb by using iron wire pliers, and sequentially cutting the three iron wires from the head to the tail, the left upper limb to the right lower limb, and the left lower limb to the right upper limb. And the three iron wires are fixed by another iron wire.
5. Filling the head, the limbs, the tail and the abdominal cavity of the Brazilian terrapin with absorbent cotton, and tightly closing the head, the limbs, the tail and the abdominal cavity along the incision of the tortoise shell after the filling; and a wire is used for fixing the Brazilian terrapin transversely.
6. The skin of the Brazilian terrapin was tightly combined with the abdominal bone plate and shaped according to the natural state (as a precursor of an animal specimen).
7. Sucking alpha ethyl cyanoacrylate with a cyanoacrylate compound injector, and injecting the alpha ethyl cyanoacrylate into the abdominal cavity of the Brazilian terrapin (animal specimen precursor) along the four limbs, the head and the tail respectively; after the drying is completed, the alpha ethyl cyanoacrylate is sucked and respectively injected into the head, the four limbs and the tail of the Brazilian terrapin.
8. Cutting off the excessive part of the iron wire for transversely fixing the Brazilian terrapin, installing the artificial eye, and drying in the shade.
The whole process of specimen preparation, the anhydrous state is guaranteed as far as possible.
Hard tortoise shells are transformed from tortoise and turtle reptiles such as Brazil tortoise, and if the traditional method for preparing animal peeling specimen is used, phenol, arsenic and other chemical substances harmful to human body are needed. If the alpha cyanoacrylate compound is used and is coated for preservation after being completely dried, the alpha cyanoacrylate compound is difficult to pass through the hard and compact shell of the Brazilian terrapin, and the tortoise which is also completely unharmed vertebrate is twisted under the condition of complete dehydration, thus seriously affecting the beauty of the finished product.
The shells of terrapin animals such as Brazilian terrapin are opened from the side, viscera and most muscles are removed, and absorbent cotton is used as a guiding medium of the alpha cyanoacrylate compound, and the alpha cyanoacrylate compound is infiltrated into the whole body of the Brazilian terrapin from the abdominal cavity through an injector. Not only effectively solves the obvious defects that the alpha cyanoacrylate compounds can not enter the muscular tissues in the abdominal cavity from the shell of the Brazilian terrapin, but also can fully utilize the alpha cyanoacrylate compounds to reduce the waste, and simultaneously solves the problem that the appearance of the Brazilian terrapin is influenced by the distortion after the complete dehydration.
Example 4 preparation of a stripped specimen of shark from black fin
Animal materials: black shark fin
The experimental steps are as follows:
1. completely soaking dead shark in 70-80 deg.C constant temperature water bath for about 4min, and taking out until skin and muscle of shark can be easily peeled off with scalpel.
2. And (3) putting the taken-out shark in absolute ethyl alcohol, and replacing the absolute ethyl alcohol after the absolute ethyl alcohol turns yellow until the absolute ethyl alcohol does not turn yellow any more.
3. Taking out the black fin shark soaked in absolute ethyl alcohol, cutting an incision (about 5cm) at the maximum abdominal circumference of the shark by using a scalpel, peeling skin and muscle outside the incision by using a forceps, and cutting off the muscle and bone of the black fin shark at the incision by using a stripping scissors after the muscle and the skin are completely separated.
4. Stripping the muscle of the black fin shark from the incision to the head of the black fin shark, and keeping the eyeball, upper jaw, lower jaw and teeth of the black fin shark (removing the skull and brain of the black fin shark); the tail of the shark was peeled from the incision down to the tail of the shark, and the tail of the shark was retained (all parts were retained as extra-corporeal parts of the animal).
5. Washing the peeled sharkskin with absolute ethyl alcohol, spreading the sharkskin in a debranning disc after washing, shearing an iron wire (the diameter is about 1.6mm) which is as long as the black fin shark by using an iron wire clamp, and inserting the blunt iron wire into the tail of the black fin shark along the upper jaw of the shark; a wire plier is used for cutting a wire which is as long as the tail fin to the mandible, and the wire is blunt ground and then inserted into the tail part of the shark along the lower jaw of the shark.
6. Filling the black fin shark from the cut by absorbent cotton, and sewing the abdominal cut of the shark by a sewing needle after filling; after suturing is complete, the form of the specimen (as an animal specimen precursor) is arranged as desired.
7. Injecting alpha-cyanoacrylate into the head, back fin, chest fin, abdomen fin, tail fin and hip fin of shark via injector, and drying completely.
The whole process of specimen preparation, the anhydrous state is guaranteed as far as possible.
The shark belongs to cartilaginous fishes, does not have a perfect skeleton system like most of hard bone fishes such as crucian carps, and can be seriously deformed and distorted after being completely dried. Moreover, the body surface of the shark is scutiched, the muscle structure is very fine, and after complete dehydration, the alpha cyanoacrylate compound cannot permeate into the muscle tissues and abdominal cavity of the shark. The traditional use of polyurethane foaming agents to make shark specimens costs thousands of dollars, so that many small dead sharks can only be sold into the market in a food-only manner at a low price. The use of the same method for preparing animal denuded specimens based on alpha cyanoacrylate compounds as teleostean fish has the advantage that the finished product has a beautiful appearance which is far from the requirements of consumers.
The invention not only achieves the aims of sealing and corrosion prevention, but also achieves the aim of plasticity by a mode of filling the bracket and then injecting the alpha cyanoacrylate compound into the shark. The method has a close inseparable relation with that the alpha cyanoacrylate compound does not generate large deformation after reacting with the absorbent cotton, which is a problem that the traditional bracket and filling method cannot solve and overcome in the non-fur vertebrates.
The technical pairs of different methods for making an apothelial vertebrate specimen are shown in the following table (taking black shark as an example):
in conclusion, the preparation method of the non-fur vertebrate specimen has the advantages of low preparation cost, no toxicity, no pollution, simple preparation method, capability of improving the quality and the preservation effect of the specimen and good social and economic benefits.
The foregoing description is only a preferred embodiment of the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A method of making a hairless vertebrate delighted animal specimen using a cyanoacrylate compound injector, comprising the steps of:
(1) treating dead bodies of the naturally occurring unhaired vertebrates with absolute alcohol;
(2) dissecting the remains of the processed non-fur vertebrate, and removing the muscle, skeleton and organs of the animal, wherein the rest parts are the external parts of the animal;
(3) fixing and filling the animal external body part in a bracket fixing and absorbent cotton filling mode, and then sewing the cut of the filled animal external body part to obtain an animal specimen precursor;
(4) extracting the alpha cyanoacrylate compound by using a cyanoacrylate compound injector, inserting a needle head of the injector into the animal specimen precursor, and injecting the alpha cyanoacrylate compound;
(5) completely drying the alpha cyanoacrylate compound to be injected;
the cyanoacrylate compound injector comprises a needle cylinder (1), a piston core rod (2) and a needle head (5), wherein a piston end (2 a) of the piston core rod (2) extends into an inner cavity of the needle cylinder from an opening part at the rear end of the needle cylinder (1), the needle head (5) is arranged at the front end of the needle cylinder (1), the inner cavity of the needle cylinder between the needle head (5) and the piston end of the piston core rod forms a negative pressure type sliding stroke cavity (1 a), the piston core rod slides forwards and backwards along the length direction of the inner cavity of the needle cylinder to form a length adjusting structure of the negative pressure type sliding stroke cavity, and the cyanoacrylate compound injector is characterized in that the needle head (5) is of a hollow structure with an opening at one end and a sealed end, the opening end of the needle head is connected with the negative pressure type sliding stroke cavity (1 a), the needle head inner cavity (53) of the needle head is communicated with the negative pressure type sliding stroke cavity (1 a), injection holes (6) communicated with the needle head inner cavity (53) are uniformly distributed on the outer wall of the needle head (5) along the circumferential direction, the inner wall of the needle cavity (53) is covered with an anti-sticking layer (52) made of Teflon material.
2. The method for preparing the delipidated vertebrate animal preparation according to claim 1, wherein there are 2 symmetrically arranged injection holes (6).
3. The method for preparing a depilatory vertebrate animal preparation for animals according to claim 1, wherein the injection holes (6) are provided on both sides of the front end of the needle lumen (53).
4. The method of claim 1, wherein the sealing end (51) of the needle (5) is pointed at its distal end.
5. The method for preparing the fur-free vertebrate animal exfoliating specimen according to claim 1, characterized in that the front end of the syringe (1) is provided with a connecting pipe (3) communicated with the negative pressure type sliding stroke cavity (1 a), the open end of the needle (5) is provided with a joint (4), the connecting pipe is inserted in the joint to form a detachable connecting structure, and the negative pressure type sliding stroke cavity (1 a) is communicated with the injection hole sequentially through the inner cavity of the connecting pipe, the inner cavity of the joint and the inner cavity of the needle to form a flow injection channel of cyanoacrylate compound.
6. The method for preparing the delipidated vertebrate animal specimen according to claim 1, wherein the plunger rod (2) has a handle (2 b) at the rear end thereof.
7. The method for preparing a depilatory vertebrate animal preparation for exfoliating animals according to claim 1, wherein the length of the needle (5) is 5-25 cm.
8. The method of claim 1, wherein step (4) further comprises injecting an α -cyanoacrylate compound into other areas of the animal specimen precursor.
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| WO2008098007A1 (en) * | 2007-02-05 | 2008-08-14 | Freedom-2, Inc. | Tissue fillers and methods of using the same |
| CN104014056B (en) * | 2014-06-17 | 2015-11-25 | 盐城市滋润医用器材厂 | A kind of preparation method of injection with low resistance |
| CN203989293U (en) * | 2014-07-07 | 2014-12-10 | 武汉市普爱医院 | Syringe |
| CN104886038B (en) * | 2015-06-08 | 2017-06-16 | 卞陆杰 | The method for making zoological specimens |
| WO2017033452A1 (en) * | 2015-08-25 | 2017-03-02 | 学校法人同志社 | Injection needle support fastener and injection needle implement |
| CN106070183A (en) * | 2016-08-25 | 2016-11-09 | 卞陆杰 | The manufacture method of zoological specimens |
| CN106417249B (en) * | 2016-09-09 | 2020-02-14 | 上海市水产研究所 | Preparation method of crab aquatic animal stripping specimen |
| CN206745475U (en) * | 2017-01-05 | 2017-12-15 | 卞陆杰 | The cyanoacrylate compound syringe made for zoological specimens |
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| Primers for Bonding Polyolefin Substrates with Alkyl Cyanoacrylate Adhesive;Yoshihisa Okamoto 等;《The Journal of Adhesion》;20060924;第81-91页 * |
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