CN106830335A - A kind of preparation method for being applied to aquaculture system water purification agent - Google Patents
A kind of preparation method for being applied to aquaculture system water purification agent Download PDFInfo
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- CN106830335A CN106830335A CN201710041960.6A CN201710041960A CN106830335A CN 106830335 A CN106830335 A CN 106830335A CN 201710041960 A CN201710041960 A CN 201710041960A CN 106830335 A CN106830335 A CN 106830335A
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- Prior art keywords
- chlorella
- water purification
- preparation
- mud
- aquaculture system
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/16—Total nitrogen (tkN-N)
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- Biotechnology (AREA)
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- Health & Medical Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Tropical Medicine & Parasitology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention proposes a kind of preparation method for being applied to aquaculture system water purification agent, specifically includes following steps：(1) chlorella is inoculated in fluid nutrient medium, temperature be 25 28 DEG C, luminous intensity be 5000 ± 500Lx, pH be 67 condition of culture under cultivate 57 days；(2) chlorella mud of the water content 40% 60% is collected by centrifugation into；(3) protective agent is added, protective agent is added in chlorella mud；(4) drying and crushing, is dried to the algae mud that step (3) is made, and makes its water content be 2% 6%.The present invention, by large-scale production, is added protective agent and dries three technical process based on chlorella, improves the survival rate of chlorella cells.To the total nitrogen of aquaculture system, total phosphorus and COD have good degradation to water purification agent of the invention.
Description
Technical field
The present invention relates to a kind of preparation method for being applied to aquaculture system water purification agent.
Background technology
As the raising that human health is realized, people are being continuously increased to the demand of aquatic products, consequently also promote China
Culture fishery fast development.But during the tradition of aquaculture body is fed, because bait utilization is low, remaining bait adds
Upper cultivated animals defecation, can make breeding water body muddy, cause body eutrophication, cause hydroplankton amount reproduction, from
And the growing environment for cultivating body is destroyed, cause cultivation body slow-growing and easily ill.In addition, long-term, substantial amounts of bait input, water
Total nitrogen, total phosphorus in body, COD increase, and can produce toxic action to cultivation body.
The advantages of microalgae preparation is with its nonhazardous, noresidue is paid close attention to by people, using the metabolism of microalgae, can
Total nitrogen, total phosphorus, the COD of breeding water body are reduced, is purified water, some microalgaes can also promote to support directly as cultivation body bait
Grow body growth.
The content of the invention
The present invention proposes a kind of preparation method for being applied to aquaculture system water purification agent, and water purification agent prepared by the method can
Effectively to reduce total nitrogen, total phosphorus, the COD of breeding water body, purify water.
The technical proposal of the invention is realized in this way：
A kind of preparation method for being applied to aquaculture system water purification agent, specifically includes following steps：
(1) chlorella is inoculated in fluid nutrient medium, is 25-28 DEG C in temperature, luminous intensity is 5000 ± 500Lx, pH
To be cultivated 5-7 days under the condition of culture of 6-7；
(2) chlorella mud of the water content in 40%-60% is collected by centrifugation into；
(3) protective agent is added, protective agent is added in chlorella mud；
(4) drying and crushing, is dried to the algae mud that step (3) is made, and makes its water content be 2%-6%.
Preferably, fluid nutrient medium described in step (1) is one kind of BG11 and BG11+ dextrose culture-mediums.
Preferably, 1ml protective agents are added in step (3) in every gram of chlorella mud, the protective agent is ascorbic acid.
Preferably, the ascorbic acid concentrations are 0.1%-0.3%.
Preferably, in step (4), chlorella mud into dry in 35-40 DEG C of air dry oven, drops in dry process
The water content of low chlorella mud.
What the present invention was produced has the beneficial effect that：The present invention adds protective agent and drying is made to cultivate chlorella
Algae powder is preserved, and can effectively improve the cell survival rate of chlorella powder, and then extend its holding time.Being applied to breeding water body can have
Effect reduces the total nitrogen of aquaculture system, total phosphorus and COD, and with high safety, convenient.
Specific embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below in conjunction with the embodiment of the present invention,
Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
A kind of preparation method for being applied to aquaculture system water purification agent that the present embodiment is provided, comprises the following steps：
(1) chlorella is inoculated in BG11 fluid nutrient mediums, is 25 DEG C in temperature, luminous intensity is 5000Lx, and pH is 6.7
Condition of culture under cultivate 7 days；
(2) chlorella mud of the water content in 40%-60% is collected by centrifugation into；
(3) protective agent is added, 0.1% ascorbic acid 1ml is added in every gram of chlorella mud；
(4) drying and crushing, is dried to the algae mud that step (3) is made, and makes its water content be 2%-6%.Dry mistake
Journey is by chlorella mud into drying 12h in 35 DEG C of air dry oven.
(5) the algae powder after drying and crushing is discharged in breeding water body, is 25 DEG C in temperature, luminous intensity is 5000Lx, pH
To be cultivated 7 days under 6.7 condition of culture.
(6) total nitrogen, total phosphorus, the COD contents of the front and rear breeding water body of detection culture；
The total nitrogen that is breeding water body before and after the algae powder for adding protective agent drying and crushing of table 1, total phosphorus, the change of COD contents and
Clearance.As can be seen that can effectively remove total nitrogen in breeding water body, total through chlorella powder prepared by the method for the invention
Phosphorus, COD.
Table 1
| Before addition (mg/L) | After addition (mg/L) | Clearance (%) | |
| Total nitrogen | 2.70 | 1.01 | 62.59 |
| Total phosphorus | 0.47 | 0.08 | 82.98 |
| COD | 36 | 16 | 55.56 |
(7) the algae powder for adding protective agent drying and crushing is carried out into cell survival with the algae powder for being not added with protective agent drying and crushing
Rate is tested.The process of test is as follows：Algae powder 0.1g is placed in the vessel, absolute ethyl alcohol 10ml homogenate is added, then add the anhydrous second of 5ml
Alcohol, filtering, finally by filtrate with absolute ethyl alcohol constant volume to 25ml.The cuvette that an optical path is 1cm is taken, leaf obtained above is injected
Green plain ethanol solution, another plus absolute ethyl alcohol is injected in the cuvette of another same specification, as control, is divided under spectrophotometer
The optical density of the pigment solution, then the chlorophyll a value as obtained by calculating are not measured with 665nm and 649nm wavelength.
Table 2 is the change record to chlorella cells survival rate.As can be seen that the bead prepared through the method for the invention
Algae flour cell survival rate is greatly improved than the filamentous cyanobacteria algae flour cell survival rate processed without the method for the invention.
Table 2
| Group | Cell survival rate |
| Unprotected dose of group | 85.44% |
| Addition protective agent group | 92.39% |
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the invention, it is all in essence of the invention
Within god and principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (5)
1. a kind of preparation method for being applied to aquaculture system water purification agent, it is characterised in that specifically include following steps：
(1) chlorella is inoculated in fluid nutrient medium, is 25-28 DEG C in temperature, luminous intensity is 5000 ± 500Lx, and pH is 6-7
Condition of culture under cultivate 5-7 days；
(2) chlorella mud of the water content in 40%-60% is collected by centrifugation into；
(3) protective agent is added, protective agent is added in chlorella mud；
(4) drying and crushing, is dried to the algae mud that step (3) is made, and makes its water content be 2%-6%.
2. a kind of preparation method for being applied to aquaculture system water purification agent as claimed in claim 1, it is characterised in that step
(1) fluid nutrient medium described in is one kind of BG11 and BG11+ dextrose culture-mediums.
3. a kind of preparation method for being applied to aquaculture system water purification agent as claimed in claim 1, it is characterised in that step
(3) 1ml protective agents are added in every gram of chlorella mud, the protective agent is ascorbic acid.
4. a kind of preparation method for being applied to aquaculture system water purification agent as claimed in claim 3, it is characterised in that described
Ascorbic acid concentrations are 0.1%-0.3%.
5. a kind of preparation method for being applied to aquaculture system water purification agent as claimed in claim 3, it is characterised in that step
(4) in, chlorella mud into dry in 35-40 DEG C of air dry oven, is reduced the water content of chlorella mud by dry process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710041960.6A CN106830335A (en) | 2017-01-20 | 2017-01-20 | A kind of preparation method for being applied to aquaculture system water purification agent |
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| CN201710041960.6A CN106830335A (en) | 2017-01-20 | 2017-01-20 | A kind of preparation method for being applied to aquaculture system water purification agent |
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| CN106830335A true CN106830335A (en) | 2017-06-13 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107986458A (en) * | 2018-01-22 | 2018-05-04 | 武汉净宇微藻科技有限公司 | A kind of method of immobilized algal cells processing aquaculture waste water |
| CN110526404A (en) * | 2019-07-24 | 2019-12-03 | 福建省微生物研究所 | A kind of aquaculture wastewater microalgae cleanser and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101967450A (en) * | 2010-11-12 | 2011-02-09 | 河南师范大学 | Method for cultivating chlorella |
| CN105254019A (en) * | 2015-11-12 | 2016-01-20 | 蚌埠市水森林生态农业科技园 | Formula for treating waste water for aquaculture |
| CN105338831A (en) * | 2013-06-26 | 2016-02-17 | 罗盖特兄弟公司 | Method for the production of a microalgal biomass of optimised sensory quality |
| KR20160102361A (en) * | 2016-08-10 | 2016-08-30 | 가톨릭관동대학교산학협력단 | Method of Hybridly Treating Acid Mine Drainage with Clay Minerals and Microalgae |
| CN107613772A (en) * | 2014-12-16 | 2018-01-19 | 赫里艾尔发展有限公司 | Compositions based on chlorella facultae, methods for their preparation and application to plants |
-
2017
- 2017-01-20 CN CN201710041960.6A patent/CN106830335A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101967450A (en) * | 2010-11-12 | 2011-02-09 | 河南师范大学 | Method for cultivating chlorella |
| CN105338831A (en) * | 2013-06-26 | 2016-02-17 | 罗盖特兄弟公司 | Method for the production of a microalgal biomass of optimised sensory quality |
| CN107613772A (en) * | 2014-12-16 | 2018-01-19 | 赫里艾尔发展有限公司 | Compositions based on chlorella facultae, methods for their preparation and application to plants |
| CN105254019A (en) * | 2015-11-12 | 2016-01-20 | 蚌埠市水森林生态农业科技园 | Formula for treating waste water for aquaculture |
| KR20160102361A (en) * | 2016-08-10 | 2016-08-30 | 가톨릭관동대학교산학협력단 | Method of Hybridly Treating Acid Mine Drainage with Clay Minerals and Microalgae |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107986458A (en) * | 2018-01-22 | 2018-05-04 | 武汉净宇微藻科技有限公司 | A kind of method of immobilized algal cells processing aquaculture waste water |
| CN110526404A (en) * | 2019-07-24 | 2019-12-03 | 福建省微生物研究所 | A kind of aquaculture wastewater microalgae cleanser and preparation method thereof |
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