CN106916728A - A kind of magnetic stirring Analysis of Drug Susceptibility instrument and matched reagent box - Google Patents

Abstract

The invention relates to the technical field of drug sensitivity analysis, in particular to a magnetic stirring type drug sensitivity analysis instrument and a matching kit. The invention is used in combination with a microcomputer, and has the advantages of simple operation, short drug sensitivity time and high result accuracy. The drug resistance of microorganisms can be judged by the final value method, and the growth status of microorganisms can be monitored during the cultivation process, and the stirring function can be added during the cultivation process to accelerate the reproduction speed of drug-resistant microorganisms and shorten the entire drug-sensitivity process. time and reduce manual steps. During the cultivation process of microorganisms, the stirring motor rotates, driving the magnet to rotate, the stirring bar rotates under the action of the rotating magnetic field, and the drug, microbial liquid and air in the drug-sensitive stirring hole are fully mixed. Accelerate the reproduction speed of microorganisms resistant to the drug and shorten the process of drug sensitivity. After cultivating for a period of time, the moving device moves to the detection device with the kit, repeats the cultivation and detection, and finally obtains the growth curve of the microorganism.

Description

A magnetic stirring type drug susceptibility analysis instrument and matching kit

technical field

The invention relates to the technical field of drug sensitivity analysis, in particular to a magnetic stirring type drug sensitivity analysis instrument and a matching kit.

Background technique

Due to the increasing drug resistance of microorganisms, infectious diseases require accurate identification of pathogenic bacteria and testing their antimicrobial susceptibility (AST) in vitro, so as to provide reference for clinical use. At present, the clinic mainly relies on manual methods or commercial analysis systems for detection. Traditional manual methods and commercial analysis systems generally use the final value method to dilute the colonies to a certain multiple, add them to antibiotic kits coated with different types and concentrations, and put them in an incubator for 24 hours. Observe the kit with naked eyes The turbidity of the liquid in the small hole, or the way to judge the drug resistance of microorganisms by means of instrument interpretation. There are many steps to operate. Moreover, the whole cultivation process has been in a static state, which is not conducive to the full contact of bacteria with drugs and nutrients, and is not conducive to the supply of oxygen during the cultivation process. The whole process takes a long time and cannot meet the clinical requirements for rapid detection of this project.

Contents of the invention

(1) Solved technical problems

Aiming at the deficiencies of the prior art, the present invention provides a magnetic stirring type drug susceptibility analysis instrument and a supporting kit, which can be detected by the final value method, and can also be monitored during the cultivation process, and the stirring function is added during the cultivation process , Microorganisms are in full contact with drugs, nutrients, and oxygen, shortening the time of the drug-sensitivity process and reducing manual steps.

(2) Technical solution

To achieve the above object, the present invention is achieved through the following technical solutions:

A magnetic stirring type drug susceptibility analysis instrument is characterized in that: it comprises a constant temperature cultivation device, a stirring device, a moving device and a detection device; The element is used to detect the air temperature in the incubator and convert the temperature value into a corresponding voltage value. The stirring device includes a stirring motor, a magnet, and a stirring bar. The magnet is connected to the rotating shaft of the stirring motor through an adapter. The stirring bar is located in the stirring hole of the supporting kit, and the moving device includes a moving motor, a displacement platform, a first photoelectric sensor, a second photoelectric sensor, a third photoelectric sensor, a fourth photoelectric sensor and a kit tray, and the moving The motor and the displacement platform are installed on the metal plate and are on the same straight line. The motor shaft is fixedly connected with a coupling through screws, and the other end of the coupling is fixedly connected to the rotating shaft of the displacement platform through screws. The detection The device includes a white LED array, an optical collimator and a photosensitive array.

Preferably, the incubator is divided into two parts, the bottom plate and the box body. The bottom plate is composed of three layers of different materials: metal plate, thermal insulation cotton, and plastic plate. The three layers of different materials are stacked, and the thermal insulation cotton is located between the metal plate and the plastic plate. The box body is made of insulation cotton and plastic board. The metal plate has high strength and supports the internal structure of the insulation box. The insulation cotton reduces the loss of heat inside the insulation box. There are fan holes on the back of the box. Fixed on the metal plate by the fixed bracket, and on the same straight line, the fan is facing the fan hole on the rear side of the incubator, and the electric heating plate, fan, and thermal element are connected to the signal control processing unit through their respective control circuits.

Preferably, the preset temperature value in the internal register of the signal control processing unit, the preset temperature value is a temperature range, the fan is in a continuous working state, the electric heating plate is in an intermittent working state, and the thermal sensor detects When the detected temperature is higher than the preset temperature value, the electric heating plate does not work. The fan will bring the cold air outside the insulation box into the box to accelerate the cooling. If the detected temperature is lower than the preset temperature value, the electric heating plate will work. , the fan brings the heat of the heating plate into the box, agitates the air inside the incubator, and mixes the hot and cold air evenly.

Preferably, there is a stirring bar in the culture hole of each supporting kit, and the number of stirring motors and magnets is determined by the number of columns of the drug-sensitive stirring holes of the matching kit. The stirring bar directly above rotates under the action of the rotating magnetic field, and the drug, microbial liquid, air, and nutrients in the drug-sensitive stirring hole are fully mixed to accelerate the reproduction of drug-resistant microorganisms and shorten the process of drug sensitivity.

Preferably, one end of the displacement platform shaft close to the motor passes through a conversion piece, and the screw is fixedly connected to the kit tray, and a plurality of kit placement areas are set on the kit tray, and the size of the kit placement areas is determined according to the size of the kit. It is determined that a plurality of circular holes are left on the panel of the kit placement area, distributed in an array, and the number, size and distance between the small holes are consistent with the drug sensitivity detection holes in the kit.

Preferably, there is a row of tooth-shaped projections on one side of the kit tray, the number of the tooth-shaped projections is consistent with the number of detection holes in the kit, and the distance between the tooth-shaped projections is the same as that in the kit. The distance between the centers of the detection holes is the same. There are two tooth-like protrusions on the other side of the kit tray, which are the first tooth-like protrusion and the second tooth-like protrusion. The distance between the first and second tooth-like protrusions is the same as that in the kit. The distance between the first row of drug sensitive stirring holes and the last row of drug sensitive stirring holes is consistent.

Preferably, the first, second, third, and fourth photoelectric sensors are through-beam photoelectric sensors, which are blocked by opaque objects, and the signal at the output end changes, and the first photoelectric sensor is fixed on one side of the metal plate. On the first fixed bracket, the second, third, and fourth photoelectric sensors are respectively fixed on the second, third, and fourth fixed brackets on the other side of the metal plate. When cultivating, the fourth photoelectric sensor is blocked by the second tooth-shaped protrusion, and the level Change, the stirring device stirs the substance in the drug-sensitive stirring hole in the first row, after a period of time, the motor rotates forward for a certain number of steps, the stirring device stirs the substance in the drug-sensitive stirring hole in the second row, and then stirs the substance in the third row and the second row in turn. The four rows go down one by one, the second tooth-shaped protrusion blocks the fourth photoelectric sensor, when the level changes, all the substances in the drug-sensitive stirring holes can be stirred, and at the same time, the motion motor moves in reverse, stirring the first row from the bottom, The penultimate line goes down in turn, and the purpose of stirring all the substances in the drug-sensitive stirring holes is achieved with the least amount of hardware.

Preferably, after the microorganisms have been cultivated for a period of time, the moving device moves to the detection position with the reagent box, the third tooth protrudes to block the first photoelectric sensor, detects the first line, the first line detection is completed, the movement motor rotates, and the fourth tooth Protrude to block the first photoelectric sensor, detect the second line, the detection of the second line is completed, and then detect the third line and the fourth line in turn, so that the culture and detection are repeated. Input the warehouse-out command, after the signal processing control unit receives the command, control the motion motor to push the reagent box tray to move towards the warehouse-out direction, the first tooth-shaped protruding stop post on the reagent box tray, the second photoelectric sensor, stop, and take the reagent box Afterwards, the warehouse entry command is input through the input terminal of the microcomputer, and the signal processing control unit receives the instruction to control the motion motor to move toward the warehouse entry direction.

Preferably, the detection device also includes a software analysis system, the number of each group of white light LED arrays is determined by the number of columns of the kit, each group of white light LED arrays corresponds to a group of photosensitive arrays, the optical alignment components are metal components, and the internal There are several cylindrical light guide holes, and the inner wall of the light guide hole is smooth, which is conducive to light reflection. Each small hole corresponds to a white light LED. When testing, the LEDs in the white light LED array light up, and the LED light passes through the optical collimation components. , after multiple reflections, a light beam with strong light intensity is formed, which irradiates the detection hole of the kit and penetrates the drug-sensitivity detection hole to reach the photosensitive array. The photosensitive array converts the received transmitted light into a cycle, and the received transmitted light Different intensities result in different cycles. When using the final value method for detection, compare the detection data with the system preset value to analyze and judge the drug sensitivity results. When monitoring during the cultivation process, the software analysis system will store the detection values in the database in the order of time, and draw the growth curve of microorganisms.

Preferably, the magnetic stirring type drug susceptibility analysis instrument is also provided with a supporting kit, the supporting kit is a porous plate with a cover, distributed in an array, divided into drug sensitive stirring holes and drug sensitive detection holes, each The drug-sensitivity stirring hole corresponds to a drug-sensitivity detection hole. The drug-sensitivity stirring hole and the corresponding drug-sensitivity detection hole are connected holes. The drug-sensitivity stirring hole contains a stirring bar, and the drug-sensitivity testing hole does not contain a stirring bar. It affects the angle and intensity of the transmitted light received by the photosensitive array. Each well except the positive control well is also coated with dried antibiotics. The antibiotics in each row of drug-sensitive stirring wells and drug-sensitive detection wells are the same. Gradient increasing or decreasing.

(3) Beneficial effects

The invention is used in conjunction with a microcomputer, and has the advantages of simple operation, short drug sensitivity time and high result accuracy. The drug resistance of microorganisms can be judged by the final value method, and the growth status of microorganisms can be monitored during the cultivation process, and the stirring function is added during the cultivation process to accelerate the reproduction speed of drug-resistant microorganisms and shorten the entire drug-sensitivity process. time and reduce manual steps. A magnetic stirring type drug susceptibility analysis instrument mentioned in the present invention includes a constant temperature culture device, a stirring device, a moving device, and a detection device. The culturing device is a constant temperature device, the temperature of which is controlled at 35-37 degrees Celsius, and includes an electric heating plate, a fan, a thermosensitive element, and an incubator. The stirring device includes a stirring motor, a magnet, and a stirring bar. The magnet is connected to the rotating shaft of the stirring motor through an adapter, and the stirring bar is located in the drug-sensitive stirring hole of the matching kit. The motion device includes a motion motor, a displacement platform, a photoelectric sensor, and a kit tray. The detection device includes a white light LED array, an optical collimation component, and a photosensitive array. The stirring device, the moving device and the detecting device are located inside the incubator. During the cultivation of microorganisms, the stirring motor rotates, driving the magnet to rotate, and the stirring bar rotates under the action of the rotating magnetic field, and the medicine, microbial liquid, air and nutrients in the stirring hole are mixed. Accelerate the reproduction speed of microorganisms resistant to the drug and shorten the process of drug sensitivity. After cultivating for a period of time, the moving device moves to the detection device with the kit, so that the cultivation and detection are repeated, and finally the growth curve of the microorganism is obtained.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only These are some embodiments of the present invention. Those skilled in the art can also obtain other drawings based on these drawings without creative work.

Fig. 1 is a schematic diagram of the first partial structure of a magnetic stirring type drug susceptibility analysis instrument;

Fig. 2 is a schematic diagram of the second partial structure of a magnetic stirring type drug susceptibility analysis instrument;

Fig. 3 is a schematic diagram of the third partial structure of a magnetic stirring type drug susceptibility analysis instrument;

Fig. 4 is the detection flowchart of embodiment 1;

Fig. 5 is the detection flowchart of embodiment 2;

Fig. 6 is the partial structure schematic diagram of supporting kit;

Figure 1-3 reference signs:

101. Bottom plate, 102. Electric heating plate, 103. Fan, 104. Thermal element, 105. Stirring motor, 106. Magnet, 107. Motion motor, 108. Displacement platform, 109. First photoelectric sensor, 110. Second photoelectric Sensor, 111. The third photoelectric sensor, 112. The fourth photoelectric sensor, 113. Kit tray, 114. White light LED array, 115. Optical alignment component, 116. Photosensitive array, 117. The first tooth-shaped protrusion, 118. 2nd dentate, 119. 3rd dentate, 120. 4th dentate, 121. 5th dentate, 121. 6th dentate, 122. 7th dentate, 123. Eighth dentate Tooth-like protrusions.

Figure 6 reference signs:

601. drug sensitivity stirring hole, 602. drug sensitivity detection hole, 603. stirring bar.

detailed description

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

Figure 1-3 shows a partial structural schematic diagram of a magnetic stirring type drug susceptibility analysis instrument, wherein Figure 1 is a partial structural schematic diagram including a part of the culture device and a stirring device, and Figure 2 is a partial structural schematic diagram of the added motion device in Figure 1, FIG. 3 is a schematic diagram of a partial structure of the added detection device in FIG. 2 . The electric heating plate 102 and the fan 103 are fixed on the bottom plate 101 through a fixing bracket, and are on the same straight line. The magnet 106 is connected to the rotating shaft of the stirring motor 105 through an adapter. Motion motor 107, displacement platform 108 are installed on base plate, are on same straight line. The motion motor 107 is fixedly connected with a coupling by screws, and the other end of the coupling is fixedly connected to the rotating shaft of the displacement platform 108 by screws. The end of the rotating shaft of the displacement platform 108 close to the motor passes through the conversion piece, and is fixedly connected to the reagent box tray 113 by screws. The first photoelectric sensor 109 , the second photoelectric sensor 110 , the third photoelectric sensor 111 , and the fourth photoelectric sensor 112 are respectively fixed on both sides of the metal plate by fixing brackets.

Example 1:

Embodiment 1 provides the detection embodiment of final value method. Figure 6 shows a schematic diagram of the partial structure of the supporting kit. Drug susceptibility testing by final value method is carried out according to the following steps.

Step 1, prepare the sample solution and inoculate the kit. Prepare a certain concentration of sample solution, and add the prepared sample solution to all wells.

Step two, cultivate. Put the prepared specimen kit into the incubator for 18-24 hours.

Step three, on-board testing. Fig. 4 shows the detection flow chart of embodiment 1. Step S11, start operation, enter step S12, the user selects the final value method detection mode through the input terminal of the microcomputer, the signal processing control unit receives the instruction from the microcomputer, sends a control signal, the stirring motor 105, and the motion motor 107 are in a static state , waiting for the next command. If the user needs to test the specimen kit on the machine, he needs to perform the operation of setting and taking the specimen. Entering step S13, it is judged whether the user inputs a specimen setting instruction through the microcomputer terminal. If the user inputs the instruction, enter step S14, the signal processing control unit sends a control signal, controls the movement motor 107 to rotate, pushes the reagent box tray 113 to move toward the direction of the warehouse, otherwise continues to wait. If the bin-out position is reached, the first tooth-shaped protrusion 117 will block the second photoelectric sensor 110 . Go to step S15, judge whether the level of the second photoelectric sensor 110 changes, if it changes, go to step S16, the signal processing control unit controls the motion motor 107 to stop moving, wait for the user to set the sample kit, and the kit is set and taken is completed. If the user takes out the specimen, the process ends. If the user puts in the specimen, go to step S17 to judge whether the user inputs a warehouse-in instruction through the input terminal of the microcomputer. If the user inputs a warehouse entry command, enter step S18, the signal processing control unit controls the movement motor 107 to rotate, and the reagent box tray 112 moves toward the warehouse entry direction. If it reaches the detection position, the third tooth-shaped protrusion 119 will block the first photoelectric sensor 109. Go to step S19, judge whether the level of the first photoelectric sensor 109 changes, if it changes, go to step S110, the signal processing control unit controls the motion motor 107 to stop at the detection position, take the row of holes at the triangular gap in Fig. 6 as the first a row of holes. The first row of drug sensitivity detection holes 602 is located directly below the optical alignment component 115 . Entering step S111 , the signal control processing unit sends a control signal to control the white light LED array 114 to light up, enable the photosensitive array 116 , and detect the first row of drug-sensitivity detection holes 602 . Store the corresponding detection hole data in the database, and send a completion command after the detection is completed. Entering step S112, the signal processing control unit controls the motion motor 107 to send the second row of drug sensitivity detection holes 602 to the detection position. Start detecting the second line. The detection of the second line is completed, and the third line and the fourth line are detected sequentially, and enter step S113 to judge whether all holes have been detected. If the detection is completed, enter step S114, compare the detection data with the system preset value, analyze Judgment of drug sensitivity results. For example, for a certain bacterium, the system presets the yin-yang cut-off value X1, and the detected data is positive if it is higher than X1, and negative if it is lower than X1. The system presets the reference concentration X2. Positive control wells are negative, indicating an error. The positive control well is positive, and the next step is judged. MIC≤X2/5, judged as sensitive (S); MIC>X2, judged as resistant (R); X2≥MIC>X2/5, judged as intermediate (I).

Example 2:

Example 2 provides an example of monitoring during cultivation. First, prepare the sample solution and inoculate the kit, the steps are the same as in Example 1. Second, on-machine culture testing. Figure 5 shows the detection flow chart of Example 2. Step S21, start running. Entering step S22, the user selects the monitoring mode through the input terminal of the microcomputer. The user enters the sample setting through the input terminal of the microcomputer, and enters step S23. The operation of setting and taking the sample is the same as the operation steps of setting and taking the sample in Embodiment 1. After the sample kit is set, go to step S24, the signal processing control unit controls the motion motor 107 to stop at the cultivation position, and the stirring motor 105 is located directly below the first row of drug-sensitive stirring holes. If the user takes out the sample kit, go to step S215. At this point, if the user inserts the specimen kit and enters step S25, the signal processing control unit controls the stirring motor 105 to rotate, and the magnet 106 fixed on the rotating shaft of the stirring motor 105 rotates to form a rotating magnetic field, driving the drug-sensitive stirring hole 601 to rotate. The rotation of the stirring bar 603 reaches the purpose of stirring. Stir for a period of time, such as stirring for 1 minute, enter step S26, judge whether the stirring time reaches 1 minute, if it reaches 1 minute, enter step S27, the signal processing control unit controls the movement of the motor 107, and sends the second row of drug-sensitive stirring holes to On top of the stirring motor, start stirring the second row. The second line of stirring is completed, and the third line, the fourth line, etc. are stirred in turn. Go to step S28, judge whether all the wells have been stirred. If stirring is done. Go to step S29, reverse stirring. Repeated forward and reverse stirring. The signal processing control unit receives the detection signal, enters step S210, and returns to the detection position for detection. The specific detection process is the same as that in Example 1. Go to step S211, judge whether the detection of all wells is completed, and then go to step S212, and repeat the cultivation and detection steps. For multiple detections, go to step S213, the software analysis system will fit the data of multiple detections into a curve, and each time a detection is added, the fitted curve will add a point, and calculate the K value, K=ΔD/ΔT, ΔD represents two times The detected data difference, ΔT represents the time difference between two detections. Enter step S214, compare the detection data with the system preset value, and analyze and judge the drug sensitivity result. For example, for a certain bacterium, the system presets the culture time T, the system presets the K value of the yin-yang boundary, reaches the culture time T, the calculated K value is higher than the system preset yin-yang boundary, and the K value is positive, and is lower than the system preset yin-yang boundary. K value is negative. The system presets the reference concentration X2. Positive control wells are negative, indicating an error. The positive control well is positive, and the next step is judged. The way of judging is the same as in Example 1.

It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is a relationship between these entities or operations. There is no such actual relationship or order between them. Furthermore, the term "comprises", "comprises" or any other variation thereof is intended to cover a non-exclusive inclusion such that a process, method, article, or apparatus comprising a set of elements includes not only those elements, but also includes elements not expressly listed. other elements of or also include elements inherent in such a process, method, article, or device. Without further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of additional identical elements in the process, method, article or apparatus comprising said element. (The columns and rows in the text are also convenient for explanation)

The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: it can still be described in the foregoing embodiments Modifications are made to the recorded technical solutions, or equivalent replacements are made to some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (10)

1. a kind of magnetic stirring Analysis of Drug Susceptibility instrument, it is characterised in that：Including incubated device, agitating device, sportswear Put and detection means；The incubated device includes electric hot plate, fan, temperature-sensitive element and incubator, and the temperature-sensitive element is used Air themperature in detection incubator, and temperature value is changed into corresponding magnitude of voltage, the agitating device includes stirring electricity Machine, magnet, stirrer, magnet are connected in the rotating shaft of stirring motor by adaptor, and the stirrer is located at matched reagent box Susceptibility poke hole in, the telecontrol equipment include motion motor, displacement platform, the first photoelectric sensor, the second photoelectric sensing Device, the examination of the 3rd photoelectric sensor, the 4th photoelectric sensor and kit pallet, the motion motor and displacement platform are arranged on gold On category plate and on the same line, the motor shaft is screwed and is connected with shaft coupling, and the other end of shaft coupling leads to Cross screw to be fixedly attached in the rotating shaft of displacement platform, the detection means includes white light LED array, optical collimating component and sense Optical arrays.

2. magnetic stirring Analysis of Drug Susceptibility instrument according to claim 1, it is characterised in that：The incubator is divided into base plate With casing two parts, base plate is made up of metallic plate, heat-preservation cotton, three layers of different materials of plastic plate, and three layers of different materials are overlapped Place, heat-preservation cotton is located between metallic plate and plastic plate, and casing is made up of heat-preservation cotton and plastic plate, metallic plate intensity is big, to protecting Incubator internal structure is played a supporting role, and heat-preservation cotton reduces the loss of incubator internal heat, and casing rear side leaves fan hole, electricity Hot plate, fan are fixed on a metal plate by fixed support, and on same straight line, fan just to thermal insulation box body on rear side of Fan hole, the electric hot plate, fan, temperature-sensitive element are connected to signal control process unit by respective control circuit.

3. magnetic stirring Analysis of Drug Susceptibility instrument according to claim 2, it is characterised in that：The signal control process list Preset temperature value in first internal register, default temperature value is a temperature range, and the fan is in continuous firing state, The electric hot plate is in discontinuous operation state, and the temperature that the temperature-sensitive element is detected is reached the standard grade higher than preset temperature value, electric hot plate Do not work, the fan brings the cold air outside incubator into casing, accelerate cooling, the temperature for detecting is less than preset temperature Value is offline, and electric hot plate work, the fan brings the heat of heating plate into casing, stirs incubator inner air, air Uniform mixing.

4. magnetic stirring Analysis of Drug Susceptibility instrument according to claim 1, it is characterised in that：The medicine of each matched reagent box There is a stirrer in quick poke hole, the number of stirring motor and magnet comes true by the susceptibility poke hole columns of matched reagent box Fixed, the stirring motor is rotated, and drives magnet rotors, the stirrer directly over magnet to occur in the presence of rotating excitation field Rotate, medicine, microbial liquid, nutritional ingredient air inside susceptibility poke hole are sufficiently mixed, accelerate micro- to the Drug-resistant Biological reproduction speed, shortens the process of susceptibility.

5. magnetic stirring Analysis of Drug Susceptibility instrument according to claim 1, it is characterised in that：In institute's translation stage rotating shaft Near motor one end by bridgeware, screw is fixedly connected kit pallet, and multiple kits are set on the kit pallet Rest area, the size of the kit rest area determines according to kit size, is left on the panel of the kit rest area Multiple circular holes, are distributed in array-like, the distance between the quantity of circular hole, size and aperture and susceptibility detection hole one in kit Cause.

6. magnetic stirring Analysis of Drug Susceptibility instrument according to claim 5, it is characterised in that：The one of the kit pallet Side have a row dentation protrude, dentation protrusion number with kit susceptibility detection hole rows number it is consistent, dentation protrude between away from Detect consistent with a distance from the center of hole from adjacent lines susceptibility in kit, kit pallet opposite side there are 2 dentations to protrude, respectively For the first dentation protrusion, the second dentation are protruded, the first row susceptibility stirring in the spacing and kit between first and second dentation protrusion Spacing between hole and last column susceptibility poke hole is consistent.

7. magnetic stirring Analysis of Drug Susceptibility instrument according to claim 1, it is characterised in that：Described first, second and third, Four photoelectric sensors are a kind of correlation type photoelectric sensors, are stopped by opaque article, and output end signal changes, institute The first photoelectric sensor is stated to be fixed on the first fixed support of metallic plate side, second and third, four photoelectric sensors fix respectively Metallic plate opposite side second and third, on four fixed supports, during culture, the 4th photoelectric sensor is protruded by the second dentation and blocked, Level changes, and agitating device stirs the material of the first row susceptibility poke hole, after a period of time, motion motor clockwise dynamic Determine step number, agitating device stirs the material of the second row susceptibility poke hole, the third line, fourth line are stirred successively successively down, second Dentation is protruded and blocks the 4th photoelectric sensor, and when level changes, the material of all susceptibility poke holes can be stirred, Motion motor counter motion simultaneously, stirs row last, row second from the bottom successively down successively, is reached with minimum hardware and stirred Mix the purpose of all susceptibility poke hole materials.

8. magnetic stirring Analysis of Drug Susceptibility instrument according to claim 7, it is characterised in that：Microculture is for a period of time After, telecontrol equipment moves to test position with kit, and the 3rd dentation is protruded and blocks the first photoelectric sensor, detection first OK, the first row detection is completed, and motion motor is rotated, and the 4th dentation is protruded and blocks the first photoelectric sensor, detects the second row, second Row detection terminates, and detects the third line, fourth line successively down successively, culture and detection so repeatedly, puts when taking kit, leads to The input input export instruction of microcomputer is crossed, after signal processing control unit receives instruction, control motion motor is pushed away Dynamic kit pallet is moved to export direction, and the first dentation on kit pallet protrudes the photoelectric sensor of bumping post second, is stopped, Put after taking kit, being input into warehouse entry by the input of microcomputer instructs, and signal processing control unit receives instruction, controls Motion motor is moved to warehouse entry direction.

9. magnetic stirring Analysis of Drug Susceptibility instrument according to claim 1, it is characterised in that：The detection means also includes Software analysis system, every group of number of white light LED array is determined that every group of white light LED array corresponds to one by the columns of kit Group photosensitive array, optical collimating component is metal parts, and inside is scratched multiple cylinder light-conductive holes, and light-conductive hole inner wall smooth has Beneficial to the reflection of light, each aperture one white light LEDs of correspondence, during detection, the LED in white light LED array is lighted, and LED light passes through Optical collimating component, by multiple reflections, forms the stronger light beam of intensity of illumination, is irradiated on the susceptibility of kit detection hole simultaneously Penetrate susceptibility detection hole and reach photosensitive array, the transmitted light that photosensitive array will be received is converted into the cycle, the transmitted light for receiving Intensity is different, and the cycle for obtaining is different, when being detected using final value method, detection data is compared with systemic presupposition value, analyzes Judge drug sensitivity tests.When being monitored in incubation, software analysis system according to the tandem of time, incites somebody to action detected value Detected value is stored in database, and is depicted as the growth curve of microorganism.

10. magnetic stirring Analysis of Drug Susceptibility instrument according to claim 1, it is characterised in that：The magnetic stirring medicine Quick analytical instrument is additionally provided with matched reagent box, and the matched reagent box is porous plate with a lid, is distributed in array-like, is divided into medicine Quick poke hole and susceptibility detection hole, each susceptibility poke hole correspondence one susceptibility detection hole, susceptibility poke hole and corresponding Susceptibility detection hole is intercommunicating pore, and susceptibility poke hole is contained within stirrer, without stirrer in susceptibility detection hole, during detection, and not shadow The angle and intensity of the transmitted light that photosensitive array is received are rung, has also been coated with by drying in each hole in addition to Positive control wells Antibiotic, the Antibiotics in each row susceptibility poke hole and susceptibility detection hole are identical, concentration in gradient increasing or decreasing.