CN106942157B - A kind of feeding method of Moringa oleifera using artificial feed - Google Patents

Abstract

A kind of Moringa Nao snout moth's larva method for breeding using man-made feeds belongs to technical field of insect feeding.The formula of man-made feeds are as follows: Moringa 99~101g of leaf dried powder, 9~11g of agar, 4.5~5.5g of yeast powder, 0.095~1.05ml of formaldehyde, 0.45~0.55g of sorbic acid, 0.38~0.42g of ascorbic acid, the particle that diameter is 0.4~0.6cm is made in 19~21mg of glucosinolates, water 500ml after mixing.Larva goes through the phase 8~11 days, and pupa time 5~9 days, the adult stage 5~7 days, specific steps included: the acquisition in Moringa Nao snout moth's larva source, the preservation of pupa and adult eclosion, the preservation of Adult worms producting eggs and ovum, raising and transfer of larva etc..Man-made feeds are easy to save, low in cost, and the palatability of Moringa Nao snout moth's larva is good, and polypide development is normal, such that high density concentrates raising Moringa Nao snout moth's larva, provide experiment worm sources to stablize.

Description

A kind of feeding method of Moringa oleifera using artificial feed

technical field

The invention belongs to the technical field of insect feeding, in particular to a feeding method for Moringa oleifera using artificial feed.

Background technique

All parts of the whole plant of Moringa oleifera can be developed and utilized and have a wide range of uses, such as ornamental, edible, medicinal, vegetable oil and beer purification, and are also ideal oil plants, honey plants, animal husbandry materials and fuelwood. Moringa oleifera (Noordablitealis) is a newly discovered small moth pest on Moringa oleifera in my country. It mainly feeds on leaves with larvae, and can eat up all Moringa oleifera leaves when the damage is serious. At present, the Moringa oleifera borer has been distributed in Yunnan Province of my country, and has broken out into disasters in some Moringa oleifera gardens, and has a tendency to gradually expand, seriously threatening the Moringa oleifera production. In order to control Moringa oleifera safely, research on the biology and control technology of Moringa oleifera should be strengthened, which requires artificial breeding of Moringa oleifera to establish a stable indoor population and solve the supply problem of experimental insect sources.

There have been reports abroad on the use of fresh Moringa leaves to raise Moringa oleifera, but this method has obvious disadvantages: Moringa leaves have short storage time, high humidity will easily rot and deteriorate, and low humidity will easily dry up, affecting the Moringa oleifera larvae. Feed; In addition, Moringa oleifera larvae eat a lot during the overeating period, so they need to be fed and cleaned in large quantities every day when they are raised with fresh leaves of Moringa oleifera, which consumes a lot of manpower and material resources.

Contents of the invention

The purpose of the present invention is to overcome the shortcoming of prior art, provide a kind of raising method of Moringa oleifera using artificial feed, to save manpower and material resources, solve the problem of supplying the insect source for testing, set up the raising method of Moringa oleifera of indoor population .

The inventive method is as follows:

1. Collect healthy Moringa oleifera larvae over 3 years old from the wild, and raise them with artificial feed until pupation under the conditions of 29-31°C, relative humidity 70%±10%, and light L:D=14:10 ;

2. Select the healthy ones from the pupae of Moringa oleifera obtained in the previous step and put them into insect cages, and store them in shading at 29～31°C and relative humidity of 70%±10%. After the adults emerge, put weights in the cage Honey water with a concentration of 4.5-5.5% is used for nutritional supplementation of adults;

3. Under the conditions of 29-31°C, relative humidity of 70%±10% and shading, put the adult insects obtained in the previous step into a gauze-sealed bottle with a male to female ratio of 1:2, and put the weight in the bottle. The honey water with a concentration of 4.5-5.5% is used for nutritional supplementation by adults. Oviposition paper is placed at the bottom of the bottle for females to lay eggs. Check the oviposition situation every day. After 2-3 days, the egg masses can be raised after they turn black;

4. Under the conditions of 29～31°C, relative humidity 70%±10%, and light L:D=14:10, place the blackened egg mass in the previous step in a bottle with fresh Moringa leaves and seal it with gauze. After 6-12 hours of cultivation, the larvae can be seen to hatch, and the ratio of the number of eggs to fresh Moringa leaves is 1-2 leaves per egg;

5. Insert the larvae hatched at the age of 1 to 2 days in the previous step into a breathable sealed glass container with artificial feed, and change the feed every 3 to 4 days until the larvae pupate. The weight of the feed put in each time is 2 to 3 times the weight of the larvae;

6. Subculture: Repeat steps 2 to 5 above;

The formula of said artificial feed is: Moringa leaf dry powder 99～101g, agar 9～11g, yeast powder 4.5～5.5g, formaldehyde 0.095～1.05ml, sorbic acid 0.45～0.55g, ascorbic acid 0.38～0.42g, glucosinolates 19~21mg, 500ml of water, mixed to make granules with a diameter of 0.4~0.6cm.

Artificial feed can be formulated as follows:

First add the weighed Moringa leaf dry powder and yeast powder into cold water with 3/5 of the total water content of the formula, stir evenly to form a suspension, and set aside;

Then pour the weighed agar powder into the pot, add 2/5 of the total water of the formula, heat to boiling and constantly stir with a glass rod;

Add sorbic acid in boiling state, mix well, stop heating, add formaldehyde to make agar liquid;

Pour the suspension into the agar liquid and cool to 60-65°C, then add weighed ascorbic acid powder, stir evenly, cool and solidify to obtain artificial feed.

The invention has the advantages that the artificial feed is easy to preserve, the cost is low, the palatability of the moringa oleifera is good, and the development of the worm body is normal, making it possible to raise the moringa oleifera in a concentrated manner, thereby stably providing an experimental insect source.

Detailed ways

See the following examples:

The larval period is 8-11 days, the pupal period is 5-9 days, and the adult period is 5-7 days. The specific steps are:

(1) Collection of Moringa oleifera moth sources

Healthy larvae over 3 instars of Moringa oleifera were collected from the wild, and raised with artificial feed until pupation at 30°C, relative humidity 70%±10%, and light conditions L:D=14:10.

(2) Preservation of pupae and emergence of adults

Collect healthy pupae of Moringa oleifera and put them in insect cages, and store them in shade at 30°C and relative humidity of 70%±10%. After the adults emerge, put honey with a weight concentration of 5% in the cages. Water for nutritional supplementation of adults.

(3) Adult egg laying and egg preservation

Under the condition of 30°C and relative humidity of 70% ± 10% under the condition of shade, the adult worms should be placed in flat round glass candy bottles with a male to female ratio of 1:2, and the mouth of the bottle should be sealed with gauze to prevent the adults from escaping. Put into the honey water that weight concentration is 5% in the bottle to supplement the nutritional capacity for the adult, place two by commercially available 80g printing paper and fold into fan-shaped (radius 10.5cm, wide 2cm) oviposition paper at the bottom of the bottle, for Females lay eggs. Check the spawning situation regularly every day, and replace the spawning paper in time.

Eggs of the same batch every day can be placed in one place. After 2 to 3 days, the egg masses can be transferred to canned bottles after they turn slightly black.

(4) Feeding and transfer of larvae

Under the condition of 30 ℃, relative humidity 70% ± 10%, light condition L: D=14: 10, the ovum mass that turns black is placed in the canning bottle (diameter 7cm, height 10cm) that fresh Moringa leaf is housed, And seal it with gauze to prevent the larvae from escaping. 80 eggs are placed in each bottle, and the ratio of the number of eggs to fresh Moringa leaves is 2 leaves per egg; larvae can be seen to hatch after 8 hours of cultivation;

Gently insert 1 to 2-day-old larvae into a finger-shaped tube (caliber 2 cm, height 7 cm) containing artificial feed with a brush, and seal it with a cotton plug. Five larvae and three feeds were placed in each tube. The feed was changed every 3 days until the larvae pupated. The feed weight that puts into every time is 2 times of larva weight.

(5) Subculture breeding

Repeat steps (2) to (4).

The formula of artificial feed used is: Moringa leaf dry powder 100g, agar 10g, yeast powder 5g, formaldehyde 1ml, sorbic acid 5g, ascorbic acid 0.4g, glucosinolate 20mg, water 500ml, make the granule of diameter 0.5cm after mixing.

The artificial feed is prepared according to the following steps:

First add the weighed Moringa leaf dry powder and yeast powder into cold water with 3/5 of the total water content of the formula, stir evenly to form a suspension, and set aside;

Then pour the weighed agar powder into the pot, add 2/5 of the total water of the formula, heat to boiling and constantly stir with a glass rod;

Add sorbic acid in boiling state, mix well, stop heating, add formaldehyde to make agar liquid;

Pour the suspension into the agar solution and cool to 65°C, then add weighed ascorbic acid powder, stir evenly, cool and solidify to obtain artificial feed.

The relevant comparison experiment data of embodiment 1 feed

Table 1 Effects of two kinds of feeds on the developmental duration of Moringa oleifera

Note: The data listed in Table 1 are the mean ± standard error, the letters in the same row indicate the results of the data t test, and the same letters indicate no significant difference at the 0.05 level.

The above data show that the feedstuffs of the embodiments can enhance the indicators of the Moringa oleifera artificially raised in the laboratory environment.

Table 2 Effects of two kinds of feeds on the growth and development of Moringa oleifera

Note: The data listed in Table 2 are the mean ± standard error, the letters in the same column indicate the results of the data t test, and the same letters indicate no significant difference at the 0.05 level.

The above data show that the embodiment feed and can stably cultivate the Moringa oleifera under the laboratory environment.

Claims (1)

1. a method for raising Moringa oleifera using artificial feed, is characterized in that by the following steps: (1), collect the healthy Moringa oleifera larvae of more than 3 instars from the field, and raise them with artificial diet under the conditions of 29～31° C., relative humidity 70%±10%, and light L:D=14:10. pupation; (2), choose the healthy person in the pupae of Moringa oleifera obtained in the previous step and put it into the insect cage, and store it in shading under the conditions of 29～31° C. and relative humidity of 70%±10%. After the adults emerge, put them in the cage. Add honey water with a weight concentration of 4.5 to 5.5% for adults to supplement nutrition; (3) Under the conditions of 29-31°C, relative humidity of 70%±10% and shading, put the adults obtained in the previous step into a bottle sealed with gauze according to the ratio of male to female ratio of 1:2. Add honey water with a weight concentration of 4.5-5.5% for adult worms to supplement nutrition. Place oviposition paper at the bottom of the bottle for female worms to lay eggs. Check the oviposition situation every day. After 2-3 days, the egg mass will turn black before proceeding to the next step. feeding; (4), under the conditions of 29～31°C, relative humidity 70%±10%, and light L:D=14:10, place the blackened egg mass in the previous step in a bottle with fresh leaves of Moringa oleifera and cover with gauze Seal the lid and culture for 6-12 hours to see the hatching of the larvae. The ratio of eggs to fresh Moringa leaves is 1-2 leaves for each egg; (5), insert the larvae hatched at the age of 1 to 2 days in the previous step into a ventilated sealed glass container with artificial feed, and change the feed every 3 to 4 days until the larvae pupate, and the feed that is put in each time The weight is 2 to 3 times the weight of the larvae; (6), subculture: repeat the above steps 2 to 5; The formula of said artificial feed is: Moringa leaf dry powder 99～101g, agar 9～11g, yeast powder 4.5～5.5g, formaldehyde 0.095～1.05ml, sorbic acid 0.45～0.55g, ascorbic acid 0.38～0.42g, glucosinolates 19~21mg, 500ml of water, mixed to make granules with a diameter of 0.4~0.6cm.