CN106942258A - Cationic surfactant composite disinfectant for animals and preparation method and application - Google Patents
Cationic surfactant composite disinfectant for animals and preparation method and application Download PDFInfo
- Publication number
- CN106942258A CN106942258A CN201710051392.8A CN201710051392A CN106942258A CN 106942258 A CN106942258 A CN 106942258A CN 201710051392 A CN201710051392 A CN 201710051392A CN 106942258 A CN106942258 A CN 106942258A
- Authority
- CN
- China
- Prior art keywords
- disinfectant
- cationic surfactant
- methane
- dithiocyano
- composite disinfectant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/48—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —S—C≡N groups
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/145—Amines having sulfur, e.g. thiurams (>N—C(S)—S—C(S)—N< and >N—C(S)—S—S—C(S)—N<), Sulfinylamines (—N=SO), Sulfonylamines (—N=SO2)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/4425—Pyridinium derivatives, e.g. pralidoxime, pyridostigmine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/22—Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of cationic surfactant composite disinfectant for animals and preparation method and application, belongs to disinfectant technical field.The present invention solves the defect that existing composite disinfectant is present.Composite disinfectant of the present invention is made up of cationic surfactant, dithiocyano-methane and water.Its preparation method is：Dithiocyano-methane is ground into powder at room temperature, stood afterwards with after aseptic distillation water dissolves；Cationic surfactant is weighed again adds sterile purified water mixing；Above two solution is mixed and completes to prepare.Composite disinfectant of the present invention can kill the bacteriums such as Escherichia coli, salmonella, streptococcus, staphylococcus aureus rapidly, concentration than existing disinfectant is lower, application cost is low and bactericidal effect is protruded, particularly to gram-positive bacteria, suitable for applied to the sterilization to animal house air, animal house ground, livestock skin, livestock drinking water or general body surface, can be widely applied for the fields such as animal husbandry.
Description
Technical field
The present invention relates to a kind of new disinfectant, and in particular to it is a kind of suitable for livestock and poultry, animal house, disinfection of drinking water containing sun
The compound disinfectant for animals of ionic surface active agent, is multipurpose disinfectant for animals of new generation.
Background technology
The development of animal husbandry has become one of principal element of one National agricultural development level of evaluation, wants to promote poultry
It is crucial that the sound development of animal husbandry, prevention and control epidemic disease, which are propagated, and sterilization can quickly eliminate pathogen, control the propagation of epidemic disease
And prevalence.In recent years, find quick, cleaning, efficiently, wide spectrum, the disinfectant of low cost, it has also become the heat of domestic and international research
Point.
In poultry industry production using it is more be chemosterilant, be broadly divided into surfactant-based, halogen（Chlorine system
Agent, Operand, bromine preparation）, aldehydes, phenols, alcohols, oxidants, acid-base class etc..All kinds of chemosterilants disappear preventative
Played an important role in terms of poison, treatment disease, improvement environment and water quality, and respectively have its advantage and disadvantage.For example：Disinfectant phenolic
（Phenol, saponated cresol）, there is killing action to most of microbe brood body, but to gemma and virus without effect, sterilization can be broken
Bad animal tissue cell；Aldehyde disinfectant（Formaldehyde, glutaraldehyde etc.）, with high-efficiency broad spectrum bactericidal action, gemma can be killed, still
There is stronger excitant to people and animals, property is unstable；Chlorine-containing disinfectant（84- thimerosals, bleaching powder etc.）, can kill bacterium,
Brood cell and virus, moreover it is possible to bleach article, but duration of efficacy is shorter, medicine is difficult to deposit long, and with stronger excitant
And corrosivity, it is necessary to it could be used after dilution, it is long-term that serious destruction etc. is caused to environment using meeting.Preferable disinfectant should
Possess wide sterilization spectrum, work fast, good stability, low toxicity, excitant are small, environmentally safe, and to humans and animals safety
Etc. a series of feature.
Surfactant is widely used the fields such as washing, sterilization, emulsification, cosmetics, printing and dyeing, oil recovery, especially closely
Quickly, the species for being approved the disinfectant Related product of listing is being continuously increased the development speed of several years with quantity.One of which sun
Ionic surface active agent performance is protruded very much, particularly in terms of sterilization：It can be positively charged in water, is adsorbed in microorganism
Surface, forms micelle, and progressively oozes the class lipid layer and albumin layer of people's cell slurry, so as to change after birth permeability, makes cell content
Beyond the region of objective existence oozes, and causes microorganism dead.While and coagulated protein, enzyme and structural proteins are denatured, the metabolism of microorganism is destroyed, will be micro-
Biology is killed.But in surfactant, although many traditional disinfection agent have very strong bactericidal action to be but not easily decomposed, with thorn
Swash the defects such as property, stability difference, be not suitable for largely using for a long time, there is significant limitation.
Dithiocyano-methane（MBT）It is a kind of efficiently to kill the sterilized chemicals of algae.The stability of disinfectant is fine, when applying
It can degrade or decompose rapidly, secondary pollution is not resulted in environment.For main bacteria present in recirculated water, fungi and
Algae (all kinds of fungies) all has efficient killing effect, and duration of efficacy is long, and the pH value and temperature range of adaptation are equal
It is wider.Water disinfection, sterilization suitable for various culture fisheries, are after the latest generation after chlorine, chlorine dioxide, two chlordantoins
Antivirus, bactericidal agent.But really there is the defect for being slightly soluble in water.
Show according to the study, the compound system of two or more surfactant can produce cooperative effect.Foreign countries are very
Already begin one's study the correlated performance of surfactant, and has related compounding patent document to have：
Ethanol and Chlorhexidine compounding（EP0707794A1）；
Hydrogen peroxide, lactic acid, Chlorhexidine, dodecyl sulphate 0.1-3% compound systems（EP1027827A1）；
20% -80% quaternary ammonium compound, 1%-15% aldehyde, 2%-15% foam surface activity agents, 9% chelating agent etc. are formulated（US
5891922 A）；
Domestic Patents documents have：
The disinfectant of the compositions such as single double-strand compound quaternary ammonium salt 0.1- 0.3%, chlorhexidine acetate 0.1-0.3% composition（CN
101537000A）；
Glutaraldehyde, quaternary ammonium salt are the compound disinfectant of active ingredient（CN 101579330A）.
Although have in recent years the above correlation compounding new product, such compound product there is also weak point, for example,
Active constituent content is of a relatively high, using not being especially extensive, it is impossible to provide disinfectant of high-efficiency broad spectrum etc..
The content of the invention
The purpose of the present invention solves the defect that existing composite disinfectant is present.
A kind of cationic surfactant composite disinfectant for animals that the present invention is provided, its by cationic surfactant,
Dithiocyano-methane and water composition.
Specifically, the composite disinfectant is consisted of the following composition：Cationic surfactant 0.02-0.10 parts by weight,
Dithiocyano-methane 0.13-0.54 parts by weight, water complement to 100 parts by weight.
Preferably, the composite disinfectant is consisted of the following composition：Cationic surfactant 0.02-0.07 parts by weight,
Dithiocyano-methane 0.13-0.18 parts by weight, water complement to 100 parts by weight.
Preferably, the composite disinfectant is consisted of the following composition：The parts by weight of cationic surfactant 0.07, two sulphur cyanogen
The parts by weight of methylmethane 0.13, water complement to 100 parts by weight.
In above-mentioned composite disinfectant：
The parts by weight are corresponding with the unit of parts by volume, i.e., when the unit of weight is kg, and parts by volume is L, and unit of weight is g
It is that parts by volume is ml.
The cationic surfactant is chlorination-N- dococylpyridinium -1- acetamides.
Present invention also offers the preparation method of above-mentioned composite disinfectant, this method comprises the following steps：
1）At room temperature, dithiocyano-methane is ground into powder, aseptic distillation water dissolves is used afterwards, stood；
2）Weigh cationic surfactant and add sterile purified water mixing, it is standby；
3）The dithiocyano-methane solution of above-mentioned preparation is mixed with cationic surfactant solution.
Preferably, in the above method：
Step 1）In, the dithiocyano-methane is 1g, and sterile purified water is 35 DEG C of 500mL, and time of repose is 10min；
Step 2）In, the cationic surfactant is chlorination-N- dococylpyridinium -1- acetamides, and weight is 1g, described
Sterile purified water is 500mL；
Step 3）In, dithiocyano-methane solution is 2 according to volume ratio with cationic surfactant solution:1 ratio mixing.
Present invention also offers application of the above-mentioned composite disinfectant in terms of sterilization, it is empty that application mainly includes animal house
Gas, animal house ground, livestock skin, the sterilization of livestock drinking water or general body surface.
Further, it is to use liquid that described composite disinfectant dilutes 16 times with sterile distilled water when in use, wherein
The final mass percent concentration of chlorination-N- dococylpyridinium -1- acetamides is about 0.004%.
Preferably, the composite disinfectant commonly sterilizes 1-2min, sterilization functions 10min in sterilization application.
The present invention is compounded using the remarkable cationic surfactant of performance with other reagents, obtains new answer
With disinfectant, this kind compounding disinfectant overcomes the common defects that existing like product is present, with advantages below：
1st, as a result of new high-effective cationic surfactant-chlorination-N- dococylpyridinium -1- acetamides（English
For sodium dodecyl chloride-AND- pyridine-1-acetamide）Compounded with dithiocyano-methane,
So that it has wide sterilization spectrum and efficient advantage.
2nd, the concentration than existing disinfectant is lower, and application cost is low and bactericidal effect is protruded, particularly to gram
The bactericidal effect of positive bacteria.
3rd, it is widely used, it is applicable to carry out disinfection to livestock and poultry, animal house and drinking-water.
The present invention can be widely applied for the fields such as animal husbandry.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1：Cationic surfactant composite disinfectant for animals
1st, (1L) is constituted：
| Compound composition | Content（g/L） | Final concentration (%) | |
| 1 | Chlorination-N- dococylpyridinium -1- acetamides | 0.67（g） | 0.07 |
| 2 | Dithiocyano-methane | 1.33（g） | 0.13 |
| 3 | Water | Add water to 1L |
2nd, preparation method：
（1）At room temperature, 1g dithiocyano-methanes are weighed, it is powdered with mortar grinder, it is dissolved in 35 DEG C of 500mL aseptic distillations
It is completely dissolved it in water, stands 10min；
（2）Weigh chlorination-N- dococylpyridinium -1- acetamides 1g and add the mixing of 500mL sterile purified waters, it is standby；
（3）By the dithiocyano-methane solution of above-mentioned preparation and chlorination-N- dococylpyridinium -1- acetamide solutions according to volume
Than for 2:1 ratio mixing, i.e. 2L dithiocyano-methanes solution adds 1L cationic surfactant solutions, stirred, quiet
Put, obtain composite disinfectant, wherein, the percentage by weight of chlorination-N- dococylpyridinium -1- acetamides and dithiocyano-methane
Concentration is respectively 0.07%, 0.13%.
3rd, application method：
Stoste is diluted 16 times with sterile distilled water（Chlorination-N- dococylpyridinium -1- acetamide final concentrations are about 0.004%）,
Common sterilization 1-2min, sterilization functions 10min.
Embodiment 2：Cationic surfactant composite disinfectant for animals
1st, (1L) is constituted：
| Compound composition | Content（g/L） | Final concentration (%) | |
| 1 | Chlorination-N- dococylpyridinium -1- acetamides | 0.4（g） | 0.04 |
| 2 | Dithiocyano-methane | 1.6（g） | 0.16 |
| 3 | Water | Add water to 1L |
2nd, preparation method：
（1）At room temperature, 1g dithiocyano-methanes are weighed, it is powdered with mortar grinder, it is dissolved in 35 DEG C of 500mL aseptic distillations
It is completely dissolved it in water, stands 10min；
（2）Weigh chlorination-N- dococylpyridinium -1- acetamides 1g and add the mixing of 500mL sterile purified waters, it is standby；
（3）By the dithiocyano-methane solution of above-mentioned preparation and chlorination-N- dococylpyridinium -1- acetamide solutions according to volume
Than for 4:1 ratio mixing, i.e. 4L dithiocyano-methanes solution adds 1L cationic surfactant solutions, stirred, quiet
Put, obtain composite disinfectant, wherein, the percentage by weight of chlorination-N- dococylpyridinium -1- acetamides and dithiocyano-methane
Concentration is respectively 0.16%, 0.04%.
3rd, application method：
Stoste is diluted 16 times with sterile distilled water（Chlorination-N- dococylpyridinium -1- acetamide final concentrations are about 0.003%）,
Common sterilization 1-2min, sterilization functions 10min.
Embodiment 3：Cationic surfactant composite disinfectant for animals
1st, (1L) is constituted：
| Compound composition | Content（g/L） | Final concentration (%) | |
| 1 | Chlorination-N- dococylpyridinium -1- acetamides | 0.2（g） | 0.02 |
| 2 | Dithiocyano-methane | 1.6（g） | 0.16 |
| 3 | Water | Add water to 1L |
2nd, preparation method：
（1）At room temperature, 1g dithiocyano-methanes are weighed, it is powdered with mortar grinder, it is dissolved in 35 DEG C of 500mL aseptic distillations
It is completely dissolved it in water, stands 10min；
（2）Weigh chlorination-N- dococylpyridinium -1- acetamides 1g and add the mixing of 500mL sterile purified waters, it is standby；
（3）By the dithiocyano-methane solution of above-mentioned preparation and chlorination-N- dococylpyridinium -1- acetamide solutions according to volume
Than for 8:1 ratio mixing, i.e. 8L dithiocyano-methanes solution adds 1L cationic surfactant solutions, stirred, quiet
Put, obtain composite disinfectant, wherein, the percentage by weight of chlorination-N- dococylpyridinium -1- acetamides and dithiocyano-methane
Concentration is respectively 0.16%, 0.02%.
3rd, application method：
Stoste is diluted 16 times with sterile distilled water（Chlorination-N- dococylpyridinium -1- acetamide final concentrations are about 0.001%）,
Common sterilization 1-2min, sterilization functions 10min.
Experimental example 1：The Disinfection Effect detection experiment of cationic surfactant composite disinfectant for animals
First, test method
With reference to GB（GB15981-1995）Method is tested, and test strain has Escherichia coli（Escherichia coli）、
Salmonella（Salmonella enteriditis）, streptococcus（Streptococcus）, staphylococcus aureus
（Staphylococcus aureus）.Specific test procedure is as follows：
1st, 10min lowest bacteria fogging-resistant concentration determinings
1.1st, test method：
With reference to GB（GB15981-1995）Method, 5 × 10 are made with PBS by indicator bacteria5-5×106CFU/mL bacteria suspension.
By 0.2% cationic surfactant（Chlorination-N- dococylpyridinium -1- acetamides）Solution and 0.2% dithiocyano first
Alkane solution is respectively adopted 1:2、1:4、1:8 ratio mixing, while every group sets the monomer that sterile purified water is diluted to same concentrations
Control.Three complex preparations are serially diluted into 5 various concentrations again with sterile purified water 2 respectively with compareing monomer again, it is each to inhale
Take 4.5mL to be separately added into test tube, 0. 5mL bacteria suspensions are then separately added into test tube, put in 20 ± 2 DEG C of water-baths, mix simultaneously
Start to clock, in after 10min, respectively taking 0.5 m L bacterium solution mixed liquors, make count plate after appropriate dilution, calculate killing rate.
1.2nd, the computational methods of killing rate：
Killing rate=（Control group bacterium number-sample sets bacterium number）/ control group bacterium number × 100%.
2nd, qualitative sterilizing test：
2.1st, test method：
With reference to GB（GB15981-1995）Qualitative sterilizing test in method, 5 × 10 are made by the bacterium solution of bacteria distribution strain5-5
×106CFU/mL bacteria suspension.The concentration of disinfectant is selected according to the minimum bacteriostatic test results of 10min, with 2 times of sterile purified water
It is serially diluted, is compared if being not added with thimerosal test tube.Plus the mL of bacteria suspension 2.5 of equivalent is in test tube, respectively at adding after bacterium
1min, 5min, 10min, 15min, 4 different intervals take out 0.5 m L, add in 4.5 mL nertralizers that (nertralizer is
PBS compositions containing 1% lecithin, 1% sodium thiosulfate, 1.5% Tween-80）, neutralize after 10 min, take out 0.5 mL and add 4.5
In mL nutrient broth pipes.The meat soup pipe of inoculated bacteria is put into 37 DEG C of 48 h of culture, PRELIMINARY RESULTS is observed, to the 7th day.Experiment is repeated
5 times, result of determination.
2.2nd, result judgement standard：
If 1. meat soup pipe is muddy, then it represents that has bacteria growing, is designated as the positive, with（+）Represent；
If 2. culture was to the 7th day, meat soup pipe is clarified, then it represents that asepsis growth is designated as feminine gender, with（-）Represent；
3. to the meat soup pipe for being difficult to judge, take 10mL to be inoculated in nutrient agar panel, smoothened with sterilizing L rods, put 37 DEG C of cultures
48h, observes colonial morphology；And smear staining microscopy, determine whether to indicate bacteria growing.There is instruction bacteria growing to be designated as the positive；
4. 5 repetitions are tested, and represent to reach sterilizing without instruction bacteria growing.
3rd, quantitative sterilizing test：
3.1st, test method：
With reference to GB（GB15981-1995）Quantitative sterilizing test in method, prepares 5 × 105-5×106CFU/mL bacterium is hanged
Liquid.The concentration that this disinfectant for animals is used is selected according to the minimum bacteriostatic tests of 10min, is again by disinfectant sterile purified water 2
Row dilution, draws 4.5 mL and adds in test tube, put in 20 ± 2 DEG C of water-baths.After fluid temperature in test tube and bath temperature are balanced,
0.5mL bacteria suspensions are added in test tube, mixes and starts to clock.Between 1min, 5min, 10min, 15min, 4 differences
Every the time, respectively take 0.5 m L bacterium solutions mixed liquor to move into 4.5m L nertralizers and mix.10 min are neutralized, viable bacteria after appropriate dilution
Count, calculate killing rate.
3.2nd, the computational methods of killing rate：
Killing rate=（Control group bacterium number-sample sets bacterium number）/ control group bacterium number × 100%.
4th, organic matter protection test：
4.1st, test method：
With reference to GB（GB15981-1995）Organic matter protection test in method, carries out count plate by bacterium solution, uses dilution
Dilution, adds calf serum, and it is finally 10% containing serum amount to make it, is 5 × 10 containing bacterium number5-5×106CFU/mL, in this, as
Test bacteria suspension.
Selection 1:The disinfectant of 2 compound proportions determines the sterilized effect in the presence of organic matter according to preceding method
Really.
4.2nd, result judgement standard：
1. least concentration and shortest time needed for the killing rate of 5 experiments is all higher than 99.9%, the disinfectant is judged in organic matter
In the presence of, valid density and the time of sterilization can be reached；
2. this valid density and time reach that the valid density of sterilization and time are same or like with quantitative sterilizing test, are judged to
Organic matter has no significant effect to disinfectant bactericidal action.
Reach that sterilization minimum effective concentration doubles the above or most short more than one times person of extended durations of action can be considered have
Significantly affect.
5th, live sterilization test
Test method：
The result for spot disinfection experimental evidence in cattle farm《Veterinary drug test requirement specification collects》Version in 2001, selection 1:2 compounding ratios
The disinfectant of example carries out disinfection experiment to the air of cowshed, ground, skin, drinking-water, and passes through colony counting method and calculate sterilization
The Disinfection Effect at rate evaluation scene, is comprised the following steps that：
(1) the sterilization experiment of cowshed air
Incubation cowshed of the on-site disinfection test on Heilongjiang Province farm in cattle farm is tested.Sterilizing before by cowshed sweep in
Only, window and passage are obturaged, because the season of field test is in October, thus the interior temperature of house is at 8-15 DEG C or so.It will disappear
Venom is diluted with distilled water into 3 various concentrations（Respectively 1:8、1:16、1:32）Experiment, is carried out with sprayer to air in house
Spraying, using plate sedimentation respectively before sterilization, sterilization after 10min, 20min, 40min, 60min sampling should be carried out, every time
Sampling is covered after opening plate lid 10min.Collection sample is cultivated into 24h in 37 DEG C of insulating boxs, finally using colony counting method
Culture counting is carried out to each sample.
(2) the sterilization experiment on cowshed ground
By thimerosal distilled water diluting, 3 kinds of concentration are respectively prepared（Respectively 1:8、1:16、1:32）Thimerosal.In cowshed
3 areas are selected, per area area (1 × 1) m2, the excrement and dirt on ground are removed, to 3 areas respectively using the difference diluted
Concentration thimerosal carries out disinfection.More than in 3 sample regions, 4 sampled points are diagonally set, each sampled point area is (10
×10)cm2, respectively before sterilization, after sterilization 10min, 20min, 40min, 60min sampling should be carried out, sampling is to sterilize every time
Cotton swab dips the aqueous solution containing nertralizer, rolls and embrocates on sampled point, it is put into containing the nertralizer aqueous solution
Test tube in (5ml/ pipes).Sample is taken back into laboratory, using the method for lifting and piping and druming, fully washes thin on lower cotton swab
Bacterium, with sterile saline by 10 times of dilutions of bacteria suspension, cultivates 24h in 37 DEG C of insulating boxs by collection sample, each sample is entered
Row culture is counted.
(3) the sterilization experiment of ox-hide skin
The mammary gland position of selection ox is tested, with 3 concentration（Respectively 1:8、1:16、1:32）Disinfectant carry out disinfection.
10min, 20min, 40min, 60min are respectively to the sampling of sterilization position before sterilization, after sterilization, by sample in 37 DEG C of insulating boxs
24h is cultivated, culture counting is carried out to each sample.
（4）The sterilizing test of cowshed drinking-water
3 drinking troughs are selected in cowshed, three disinfectants are diluted to 1 respectively:8、1:16、1:32 3 concentration gradients.It
Afterwards from each drinking trough respectively take out 91 L water be put into it is standby in sterilizing bottle, then each disinfectant respectively using dilution
Three different concentration carry out disinfection experiment to the drinking-water in sterilizing bottle, in 10 min, 20 min, 40 before sterilization, after sterilization
Min, 60 min are taken, and the water sample for suctioning out 5ml with sterilized straw during collection is put into sterilizing test tubes.Sample will be gathered in laboratory
Tested, 24 ~ 48h is cultivated in 37 DEG C of insulating boxs, culture counting is carried out to each sample.
2nd, result of the test
1st, 10min lowest bacteria fogging-resistant concentration determinings result
Make minimum inhibitory concentration detection for Escherichia coli, salmonella, streptococcus and staphylococcus aureus respectively, specific inspection
Survey result as follows：
1.1st, the minimum inhibitory concentration testing result to Escherichia coli is shown in Table 1：
Table 1, the lowest bacteria fogging-resistant concentration determining result to Escherichia coli
Note：It is 5 × 10 to originate bacterium number6CFU/mL." a in " sequence number " of form first row）" represent 1:2 compound proportions, " b）" table
Show 1:4 compound proportions, " c）" represent 1:8 compound proportions.In table, Cationic represents cationic surfactant, and MBT represents two
Thiocyanogen methane disinfectant.
1.2nd, the minimum inhibitory concentration testing result to salmonella is shown in Table 2：
Table 2, the lowest bacteria fogging-resistant concentration determining result to salmonella
Note：It is 5 × 10 to originate bacterium number6CFU/mL." a in " sequence number " of form first row）" represent 1:2 compound proportions, " b）" table
Show 1:4 compound proportions, " c）" represent 1:8 compound proportions.
1.3rd, 3 are shown in Table to streptococcic minimum inhibitory concentration testing result：
Table 3, to streptococcic lowest bacteria fogging-resistant concentration determining result
Note：It is 5 × 10 to originate bacterium number6CFU/mL." a in " sequence number " of form first row）" represent 1:2 compound proportions.“b）" table
Show 1:4 compound proportions, " c）" represent 1:8 compound proportions.
1.4th, the minimum inhibitory concentration testing result to staphylococcus aureus is shown in Table 4：
Table 4, the lowest bacteria fogging-resistant concentration determining result to staphylococcus aureus
Note：It is 5 × 10 to originate bacterium number6CFU/mL." a in " sequence number " of form first row）" represent 1:2 compound proportions, " b）" table
Show 1:4 compound proportions, " c）" represent 1:8 compound proportions.
It can determine according to above-mentioned table 1-4 result：
The disinfectant that cationic surfactant is compounded with dithiocyano-methane is 1:During 2 compound proportion, to Escherichia coli, sramana
Minimum extension rate sterilized Salmonella 10min is 1:32（Cationic surfactant is with dithiocyano-methane disinfectant 1:2
Minimum inhibitory concentration during compound proportion is followed successively by 0.002%, 0.004%）；It is sterilized to streptococcus, staphylococcus aureus 10min
Minimum extension rate be 1:64（Cationic surfactant is with dithiocyano-methane disinfectant 1:During 2 compound proportion
Minimum inhibitory concentration is followed successively by 0.001%, 0.002%）.
According to above-mentioned testing result, the sterilization effect that kills of control group is not so good as composite disinfectant of the invention, and this, which is combined, disappears
Toxic agent is protruded gram-positive bacteria bactericidal effect very much.
2nd, the qualitative disinfection test result to different bacterium is shown in Table 5：
Table 5, to the qualitative disinfection test result of different bacterium
Note：Starting bacterium number is 5x106CFU/mL.Control group is+.
The result of table 5 is shown：According to the above-mentioned minimum bacteriostatic test results of 10min, selection 1:The present invention of 2 compound proportion
Described composite disinfectant stoste does appropriate dilution, the qualitative sterilizing test for the agent that carries out disinfection afterwards.For Escherichia coli and sand
Door Salmonella, uses this disinfectant 1:Reach that sterilizing is required in 32 times of dilution 10min times；To streptococcus and staphylococcus aureus
Killing effect preferably, uses this disinfectant 1：64 times are diluted in 1min and can reach sterilizing requirement.
3rd, quantitative disinfection test result is referring to table 6：
Table 6, to the quantitative disinfection test result of different bacterium
Note：It is 5 × 10 to originate bacterium number and control group bacterium number6 CFU/mL。
The result of table 6 is shown, for Escherichia coli and salmonella, and composite disinfectant of the present invention is according to 1:32 times dilute
Release, reach that sterilization is required in 1min；To streptococcus and staphylococcus aureus killing effect preferably, this disinfectant 1:64 times dilute
Release, 1min is that can reach sterilization to require that sterilizing rate is more than 99.9%.
4th, organic matter protection test the results are shown in Table 7：
Table 7, to different bacterium organic matter protection test result
Note：It is 5 × 10 to originate bacterium number and control group bacterium number6 CFU/mL。
The result of table 7 shows that composite disinfectant of the present invention is under organic matter effect, to Escherichia coli and Salmonella
Bacterium, 1:32 times reach that sterilization is required when being diluted in 1min；It is preferable to streptococcus and staphylococcus aureus killing effect.The present invention
The 1 of described composite disinfectant:64 times can reach sterilization and require when being diluted in 1min, sterilizing rate is more than 99.99%, with disappearing
The quantitative disinfection test result of toxic agent is close, illustrates that composite disinfectant of the present invention is not influenceed by organic matter, therefore, it is determined that
Composite disinfectant bactericidal action of the present invention is had no significant effect for organic matter.
5th, the case study on implementation that composite disinfectant of the present invention is applied at the scene
Field test is according to above result of the test, selection 1:The composite disinfectant of 2 compound proportions carries out on-the-spot disinfection checking test
It is specific as follows：
5.1st, 8 are shown in Table to cowshed air sterillization result of the test：
Table 8, to cowshed air sterillization result of the test
Note：Bacterial population is 1.8 × 10 before sterilization5 CFU/mL
The result of table 8 shows that cationic surfactant compounds disinfectant with dithiocyano-methane, in 60min action times, 1:16
Killing rate is that can reach 99.9% under the concentration of dilution again（Cationic surfactant and dithiocyano-methane decontaminant concentration according to
Secondary is 0.004%, 0.008%）, and monomer disinfectant is not up to the standard.
5.2nd, composite disinfectant of the present invention is shown in Table 9 to cowshed ground sterilization result of the test：
Table 9, composite disinfectant are to cowshed ground sterilization result of the test
Note：Bacterial population is 2.9 × 10 before sterilization5 CFU/mL。
The result of table 9 shows that cationic surfactant compounds disinfectant with dithiocyano-methane, in 20min action times,
1:Killing rate under the concentration of 8 times of dilutions is 99.96%（Cationic surfactant and dithiocyano-methane decontaminant concentration according to
Secondary is 0.008%, 0.016%）, other concentration can not reach the standard of killing rate >=99.9%, and monomer disinfectant not up to judges
Standard.
5.3rd, composite disinfectant of the present invention is shown in Table 10 to ox-hide skin disinfection test result：
The result of the test of table 10, composite disinfectant to ox body skin degerming
Note：Bacterial population is 1.1x10 before sterilization5 CFU/mL
The result of table 10 shows that cationic surfactant compounds disinfectant under 20min action times with dithiocyano-methane, 1:
>=99.96% criterion is can reach under the concentration of 8 times of dilutions（Cationic surfactant is sterilized with dithiocyano-methane
Agent concentration is followed successively by 0.008%, 0.016%）, the not up to criterion of monomer disinfectant.
5.4th, composite disinfectant of the present invention to disinfection of drinking water result of the test to being shown in Table 11：
The result of the test of table 11, composite disinfectant to disinfection of drinking water
Note：Bacterial population is 1.1x10 before sterilization5 CFU/mL。
The result of table 11 shows that cationic surfactant compounds disinfectant in 40min action times with dithiocyano-methane
Under, 1:>=99.90% criterion is can reach under the concentration of 8 times of dilutions（Cationic surfactant and dithiocyano-methane
Decontaminant concentration is followed successively by 0.008%, 0.016%）, the not up to criterion of monomer disinfectant.
Based on the above results, illustrate cationic surfactant（Chlorination-N- dococylpyridinium -1- acetamides）With two
Thiocyanogen methane disinfectant is according to 1:2 ratio compounding is used in combination, and can produce synergistic function can expand fungicidal spectrum.Together
When, the sterilized ability of dithiocyano-methane had both been strengthened, cationic surfactant is reduced again（Chlorination-N- dodecyl pyrroles
Pyridine -1- acetamides）Concentration, reduce cost.
Although, above with general explanation, embodiment and experiment, to composite disinfecting of the present invention
The performance and effect of agent have made detailed description, but on the basis of the present invention, it can be made some modifications or improvements, and this is to this
It is obvious for art personnel.Therefore, made without departing from theon the basis of the spirit of the present invention these modification or
Improve, belong to the scope of protection of present invention.
Claims (10)
1. a kind of cationic surfactant composite disinfectant for animals, it is characterised in that it is by cationic surfactant, two sulphur
Cyano group methane and water composition.
2. composite disinfectant according to claim 1, it is characterised in that the cationic surface in the composite disinfectant is lived
Property agent is that 0.02-0.10 parts by weight, dithiocyano-methane are 0.13-0.54 parts by weight, and water complements to 100 parts by weight.
3. composite disinfectant according to claim 1, it is characterised in that the composite disinfectant cationic surface-active
Agent is that 0.02-0.07 parts by weight, dithiocyano-methane are 0.13-0.18 parts by weight, and water complements to 100 parts by weight.
4. composite disinfectant according to claim 1, it is characterised in that the composite disinfectant cationic surface-active
Agent is that 0.07 parts by weight, dithiocyano-methane are 0.13 parts by weight, and water complements to 100 parts by weight.
5. the composite disinfectant according to claim 1-4 any one claims, it is characterised in that the cation form
Face activating agent is chlorination-N- dococylpyridinium -1- acetamides.
6. a kind of method of the composite disinfectant prepared described in claim 1, it is characterised in that this method comprises the following steps：
1）At room temperature, dithiocyano-methane is ground into powder, aseptic distillation water dissolves is used afterwards, stood and form two sulphur cyanogen
Methylmethane solution；
2）Weigh cationic surfactant and add sterile purified water mixing, form cationic surfactant solution；
3）The dithiocyano-methane solution of above-mentioned preparation is mixed with cationic surfactant solution.
7. preparation method according to claim 6, it is characterised in that：
Step 1）In, the dithiocyano-methane is 1g, and sterile purified water is 35 DEG C of 500mL, and time of repose is 10min；
Step 2）In, the cationic surfactant is chlorination-N- dococylpyridinium -1- acetamides, weighs weight for 1g,
The sterile purified water is 500mL；
Step 3）In, dithiocyano-methane solution is 2 according to volume ratio with cationic surfactant solution:1 ratio mixing.
8. application of the composite disinfectant described in claim 1-5 any one in terms of sterilization.
9. application according to claim 8, it is characterised in that the application includes animal house air, animal house ground, livestock skin
The sterilization of skin, livestock drinking water or general body surface.
10. application according to claim 8, it is characterised in that the application is by composite disinfectant sterile distilled water
16 times of dilution, commonly sterilizes 1-2min, sterilization functions 10min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710051392.8A CN106942258A (en) | 2017-01-23 | 2017-01-23 | Cationic surfactant composite disinfectant for animals and preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710051392.8A CN106942258A (en) | 2017-01-23 | 2017-01-23 | Cationic surfactant composite disinfectant for animals and preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106942258A true CN106942258A (en) | 2017-07-14 |
Family
ID=59466173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710051392.8A Pending CN106942258A (en) | 2017-01-23 | 2017-01-23 | Cationic surfactant composite disinfectant for animals and preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106942258A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107509740A (en) * | 2017-08-16 | 2017-12-26 | 黑龙江省兽医科学研究所 | A kind of cationic surfactant composite disinfectant |
| CN110638864A (en) * | 2019-10-10 | 2020-01-03 | 山西益鑫泰生物科技有限公司 | Traditional Chinese medicine compound preparation and application thereof in prevention and treatment of animal epidemic diseases |
| CN114246194A (en) * | 2020-09-24 | 2022-03-29 | 湖南五指峰生化有限公司 | Disinfectant for livestock and poultry breeding and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798775A (en) * | 2015-05-06 | 2015-07-29 | 黑龙江八一农垦大学 | Animal compound disinfectant with cationic surface active agent |
| CN104886110A (en) * | 2015-05-07 | 2015-09-09 | 黑龙江八一农垦大学 | Multipurpose composite cationic surfactant type disinfectant |
-
2017
- 2017-01-23 CN CN201710051392.8A patent/CN106942258A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798775A (en) * | 2015-05-06 | 2015-07-29 | 黑龙江八一农垦大学 | Animal compound disinfectant with cationic surface active agent |
| CN104886110A (en) * | 2015-05-07 | 2015-09-09 | 黑龙江八一农垦大学 | Multipurpose composite cationic surfactant type disinfectant |
Non-Patent Citations (1)
| Title |
|---|
| 项成林: "SQ8新型杀菌灭藻剂", 《净水技术》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107509740A (en) * | 2017-08-16 | 2017-12-26 | 黑龙江省兽医科学研究所 | A kind of cationic surfactant composite disinfectant |
| CN110638864A (en) * | 2019-10-10 | 2020-01-03 | 山西益鑫泰生物科技有限公司 | Traditional Chinese medicine compound preparation and application thereof in prevention and treatment of animal epidemic diseases |
| CN114246194A (en) * | 2020-09-24 | 2022-03-29 | 湖南五指峰生化有限公司 | Disinfectant for livestock and poultry breeding and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Oule et al. | Akwaton, polyhexamethylene-guanidine hydrochloride-based sporicidal disinfectant: a novel tool to fight bacterial spores and nosocomial infections | |
| CN101810178B (en) | Disinfectant for culturing silkworms | |
| CN102228058B (en) | Citric acid composite disinfectant | |
| CN103766399B (en) | A kind of iodine-containing disinfectant and preparation method thereof | |
| CN102067870A (en) | Composition of disinfectant and preparation method thereof | |
| CN101700011A (en) | Compound disinfectant for livestock and poultry raising environment | |
| Pan et al. | Efficient inactivation of African swine fever virus by a highly complexed iodine | |
| CN106942258A (en) | Cationic surfactant composite disinfectant for animals and preparation method and application | |
| Tyski et al. | Animal Health Protection–Assessing Antimicrobial Activity of Veterinary Disinfectants and Antiseptics and Their Compliance with European Standards: A Narrative Review. | |
| CN107836466A (en) | A kind of tea tree ethereal oil natural disinfectant agent for animals and preparation method thereof | |
| CN104798775B (en) | A kind of cationic surfactant composite disinfectant for animals | |
| CN106386853A (en) | Multi-effect active iodine disinfectant as well as preparation method and application thereof | |
| CN106135202A (en) | The preparation method of a kind of foamaseptic and using method thereof | |
| US20200128822A1 (en) | Hyperprotonation Compositions And Methods Of Use For Cleaning, Disinfection, And Sterilization | |
| CN104886110B (en) | A kind of multiduty composite cation surfactant disinfectants | |
| CN102939966B (en) | A kind of compound disinfectant, its preparation method and application | |
| CN102106347A (en) | Cinnamaldehyde, eugenol, thymol and tannin compound bactericide | |
| CN108935526A (en) | A kind of cordate houttuynia silver ion composite disinfectant | |
| CN102321728A (en) | Evaluation method for disinfection effect of disinfectant | |
| CN109699686A (en) | A kind of disinfectant for killing avian influenza virus | |
| Turabekov et al. | Enhancing animal welfare and immune health: a study on hydrogen peroxide and iodine-based disinfectants in farms | |
| CN111418602A (en) | No-clean disinfectant and preparation method and application thereof | |
| CN106973902A (en) | It is a kind of to kill cultivated spore preparation containing amino acid derivativges | |
| CN107509740A (en) | A kind of cationic surfactant composite disinfectant | |
| Obi et al. | Antimicrobial activities of some household Disinfectants on selected human pathogens in Umuahia, Abia state, Nigeria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170714 |