CN106969813A - A kind of high-precision pipettor automatic calibrator - Google Patents

Abstract

The invention discloses a kind of high-precision pipettor automatic calibrator, including pipettor moving parts, liquid weighing component, suction nozzle storage assembly for storing and device pedestal.Pipettor moving parts include down-feed screw and horizontal lead screw, and the top of down-feed screw is connected with motor vertical, and the side of horizontal lead screw is connected with the sliding block also set up on horizontal motor, down-feed screw for installing pipettor to be measured；Liquid weighing component includes being provided with liquid container on high accuracy analysis balance, high accuracy analysis balance；Suction nozzle storage assembly for storing includes the suction nozzle box support for being used to deposit disposable liquid-transfering sucker.The device possesses the pipettor moving parts of horizontal and vertical two dimensional motion ability by design, liquid weighing component and suction nozzle charging assembly are combined can realize the pipettor calibrating installation of automation in the same apparatus, improve calibrating efficiency, reduce gauging error, expand calibrating scope so that pipettor verification result has more confidence level.

Description

A high-precision pipette automatic verification device

technical field

The invention relates to a pipette verification technology, in particular to an automatic verification device for a high-precision pipette used to verify whether a pipette to be tested conforms to relevant national (international) standards.

Background technique

In biochemical experiments and clinical testing, the pipette is one of the core devices of the entire experimental process, and the pipetting accuracy of the pipette directly affects the results of the entire experiment and detection. Unqualified pipetting accuracy will not only increase the experimental error, but even lead to experimental failure in biochemical experiments that require high precision. Whether it is a commercial pipette or a pipette engineering machine designed in the laboratory, it is very necessary to check whether the pipetting accuracy meets the relevant national (international) standards. The existing pipette automatic verification device is only a fixed platform, which does not have horizontal and vertical two-dimensional movement capabilities, and does not integrate the liquid weighing component and the tip loading component in the same system, so it is often necessary to first Place the tip loading module on the platform to load the tips, remove the tip loading module after the tip loading is completed, and then manually place the liquid weighing module on the platform, and only automatic pipettes with vertical movement capabilities can be tested , the test range is small. For the verification process with a large number of repetitive operations, this will be very time-consuming and manpower-consuming, and may introduce manual errors, which will affect the accuracy of the verification.

Contents of the invention

Purpose of the invention: Aiming at the problems existing in the prior art, the present invention provides an automatic verification device for high-precision pipettes. By designing a pipette movement component with horizontal and vertical two-dimensional movement capabilities, the device combines the liquid weighing component and the tip loading component in the same device to realize an automated pipette verification device, which improves the verification efficiency and reduces the verification cost. Errors, expanding the scope of the test, making the pipette test results more credible.

Technical solution: In order to achieve the above object, a high-precision pipette automatic verification device according to the present invention is characterized in that it includes a pipette movement component, a liquid weighing component, a tip storage component and a device base; The liquid weighing assembly and the tip storage assembly are installed side by side on the base of the device, and the pipette movement assembly is arranged behind the liquid weighing assembly and the tip storage assembly; the pipette movement assembly includes a vertical lead screw and a horizontal lead screw , the top of the vertical lead screw is connected with a vertical motor, the side end of the horizontal lead screw is connected with a horizontal motor, and the vertical lead screw is also provided with a slider for installing the pipette to be tested; the liquid weighing assembly includes a high-precision analysis A balance, a high-precision analytical balance is provided with a liquid container; the tip storage assembly includes a tip box bracket for storing disposable pipette tips.

The pipette movement assembly is used to fix and drive the pipette to be tested, wherein the vertical motor drives the pipette to move up and down, and the horizontal motor drives the pipette to move horizontally.

The liquid weighing component is used to weigh the test liquid with high precision and record the results, wherein the high-precision analytical balance weighs the liquid to be tested, and the liquid container is used to hold the liquid to be tested.

The tip storage component is used to store disposable tips for pipetting.

In the pipette motion component, there are independent vertical motion modules and horizontal motion modules, so that the high-precision pipette automatic verification device has a two-dimensional motion capability, and the independent liquid weighing component and the tip storage component are combined in the In the same device, the automatic process of pipette verification is realized.

Further, an upper limit sensor and a lower limit sensor are provided on the side of the vertical lead screw. The upper limit sensor and the lower limit sensor are used to limit the range of up and down movement of the pipette.

Further, an anti-evaporation trap is arranged on the top of the liquid container. Evaporation traps are used to prevent liquids from evaporating.

Further, the moving assembly of the pipette is also provided with a fixed assembly, one end of which is fixed on the high-precision analytical balance, and the other end is fixed on the base of the device. The fixed component is used to prevent the high-precision analytical balance from moving during the pipette verification process, which will affect the weighing operation. The fixing component is a "Z" shaped iron frame, and the high-precision analytical balance and the device base are respectively fixed by screws.

Further, a tip box positioning block is provided at the bottom of the tip box support. The positioning block is used to determine the position of the tip rack holder at the beginning of each pipette verification.

Further, the four screws of the pipette to be tested are fixed on the slider of the vertical screw, and the slider and the vertical screw are engaged through threads.

Working principle: Step 1: This device first fixes the pipette to be tested on the slider located on the vertical screw with four screws. The slider and the vertical screw are engaged through threads. When the vertical motor drives the vertical screw to rotate It can drive the slider to move up and down at the same time, so that the pipette to be tested fixed on the slider has the ability to move in the vertical direction, wherein the upper limit sensor is used to limit the upper limit of the movement of the pipette to be tested, and the lower limit sensor Used to limit the lower limit of movement of the pipette under test

Step 2: After the installation of the pipette to be tested is completed, the device drives the horizontal screw to rotate through the horizontal motor to make the pipette to be tested horizontally. The pipette to be tested first moves horizontally to a fixed position above the tip box bracket, and then Vertical downward movement until the pipette under test is successfully loaded with disposable pipette tips stored on the tip rack holder, where the positioning block is used to determine the placement position of the tip rack rack at the beginning of each pipette verification ;

Step 3: After the disposable pipette tip is loaded, the device drives the pipette to be tested to move horizontally to a fixed position above the high-precision analytical balance, and then the pipette to be tested moves vertically downward, so that the disposable pipette tip enters Draw the test liquid into the liquid container containing the test liquid. After the suction is completed, the pipette to be tested moves vertically upwards to the initial position, and the high-precision analytical balance performs a tare operation. After the tare operation is completed, the pipette to be tested moves again. Move vertically down to the liquid container to release the test liquid, and move upward to return to the initial position. The high-precision analytical balance weighs the released test liquid and records the weight of the test liquid. The anti-evaporation trap is used to prevent the liquid container from The evaporation of the test liquid in the pipette affects the weighing, and the fixed components are used to prevent the high-precision analytical balance from moving during the pipette verification process, which affects the weighing operation.

Step 4: Move the pipette to be tested horizontally to the designated position, and unload the disposable pipette tip.

Steps 1-4 are a complete pipetting and weighing process. Repeating steps 2-4 can obtain multiple sets of pipetting and weighing data, and perform statistical analysis to calculate the pipetting accuracy of the pipette to be tested (2), and verify Whether it complies with relevant national (international) standards.

Beneficial effect: compared with the prior art, the present invention has the following advantages:

The invention combines the pipetting operation and weighing operation in the pipette verification process in the same device through the combined use of the pipette movement component, liquid weighing component and tip storage component, and realizes the pipette verification automated process. The horizontal movement capability of the device enables the liquid weighing component and the tip storage component to be combined and used on the device at the same time, which improves the degree of automation of the device; the vertical movement capability makes it possible for the pipette to be tested without the ability to move up and down. The automatic verification of the pipette can also be realized in the designed automatic device, which expands the verification range of the pipette and improves the verification ability of the device.

Description of drawings

Fig. 1 is the structural representation of a kind of high-precision pipette automatic verification device of the present invention;

Fig. 2 is the front view of a kind of high-precision pipette automatic verification device of the present invention;

Fig. 3 is the left view of Fig. 2;

FIG. 4 is a top view of FIG. 2 .

detailed description

The present invention is further described below in conjunction with embodiment and accompanying drawing.

Example

As shown in Figure 1-4, a high-precision pipette automatic verification device includes a pipette movement component 1, a liquid weighing component 2, a tip storage component 3 and a device base 4; a liquid weighing component 2, a suction The head storage components 3 are installed side by side on the device base 4 , and the pipette movement component 1 is arranged behind the liquid weighing component 2 and the tip storage component 3 .

The pipette movement assembly 1 includes a vertical lead screw 5 and a horizontal lead screw 6, the top of the vertical lead screw 5 is connected with a vertical motor 7, the side end of the horizontal lead screw 6 is connected with a horizontal motor 8, and the vertical lead screw 5 is also provided with a To install the slide block 10 of the pipette 9 to be tested, the pipette 9 to be tested is fixed on the slide block 10 of the vertical lead screw 5 by four screws, the slide block 10 and the vertical lead screw 5 are threadedly engaged, and the vertical lead screw The 5 sides are provided with an upper limit sensor 15-1 and a lower limit sensor 15-2.

The liquid weighing assembly 2 includes a high-precision analytical balance 11, and a liquid container 12 is arranged on the high-precision analytical balance 11, and an anti-evaporation trap 16 is arranged on the top of the liquid container 12. The pipette movement assembly 2 is provided with a fixed assembly 17 to prevent the balance from moving. The fixed assembly 17 is a "Z" shaped iron frame fixed on the high-precision analytical balance 11 by screws at one end, and the other end is fixed on the device base 4.

The tip storage assembly 3 includes a tip box support 14 for storing disposable pipetting tips 13 , and a tip box positioning block 18 is provided at the bottom of the tip box support 14 .

This device firstly fixes the pipette 9 to be tested on the slider 10 located on the vertical screw 5 through four screws, the slider 10 and the vertical screw 5 are threadedly engaged, when the vertical motor 7 drives the vertical screw 5 to rotate When just can drive slide block 10 to move up and down simultaneously, thereby impel the pipette 9 to be tested to be fixed on the slide block 19 to have the ability to move in the vertical direction, wherein upper limit sensor 15-1 is used for limiting the pipette to be tested The upper limit of movement, the lower limit sensor 15-2 is used to limit the lower limit of movement of the pipette to be tested

After the installation of the pipette 9 to be tested is completed, the device drives the horizontal screw to rotate through the horizontal motor 8 to make the pipette 9 to move horizontally, and the pipette 9 to be tested first moves horizontally to a fixed position above the tip box bracket 14 , and then move vertically downward until the pipette 9 to be tested successfully loads the disposable pipette tips 13 stored on the tip box holder 14, wherein the tip box positioning block 18 is used to determine the beginning of each pipette test When the placement position of the tip box support 14;

After the disposable pipette tip 13 is loaded, the device drives the pipette 9 to move horizontally to a fixed position above the high-precision analytical balance 11, and then the pipette 9 to be tested moves vertically downward, so that the disposable pipette tip 13 Enter the liquid container 12 containing the test liquid and suck the test liquid. After the suction is completed, the pipette 9 to be tested moves vertically upwards to the initial position, and the high-precision analytical balance 11 performs the tare operation. After the tare operation is completed, wait The measuring pipette 9 moves vertically downward again to the liquid container 12 to release the test liquid, and moves upward to return to the initial position, and the high-precision analytical balance 11 weighs the released test liquid and records the weight of the test liquid, wherein The anti-evaporation trap 16 is used to prevent the test liquid in the liquid container from evaporating from affecting the weighing, and the fixing component 17 is used to prevent the high-precision analytical balance from moving during the verification process of the pipette and affect the weighing operation.

The pipette 9 to be tested moves horizontally to the rightmost end of the verification device, unloads the disposable pipetting tip 13, and completes a complete pipetting and weighing process. Repeat all the above steps to obtain multiple sets of pipetting and weighing data, and perform statistical analysis to calculate the pipetting accuracy of the pipette to be tested, and verify whether it meets the relevant national (international) standards, that is, JJG 646-2006 pipette Verification regulations and ISO 8655International Standard.

Claims (8)

1. a kind of high-precision pipettor automatic calibrator, it is characterised in that including pipettor moving parts (1), liquid weighing Component (2), suction nozzle storage assembly for storing (3) and device pedestal (4)；The liquid weighing component (1), suction nozzle storage assembly for storing (3) are side by side On device pedestal (4), pipettor moving parts (1) are arranged on after liquid weighing component (2) and suction nozzle storage assembly for storing (3) Side；The pipettor moving parts (1) include down-feed screw (5) and horizontal lead screw (6), and the top of down-feed screw (5) is connected with Motor vertical (7), the side of horizontal lead screw (6) is connected with to also set up on horizontal motor (8), down-feed screw (5) and treated for installation Survey the sliding block (10) of pipettor (9)；The liquid weighing component (2) includes high accuracy analysis balance (11), high accuracy analysis day Liquid container (12) is provided with flat (11)；The suction nozzle storage assembly for storing (3) includes being used to deposit disposable liquid-transfering sucker (13) Suction nozzle box support (14).

2. high-precision pipettor automatic calibrator according to claim 1, it is characterised in that the down-feed screw (5) Side is provided with upper limit level sensor (15-1) and lower limit level sensor (15-2).

3. high-precision pipettor automatic calibrator according to claim 1, it is characterised in that the liquid container (12) Top is provided with vaporization prevention trap (16).

4. high-precision pipettor automatic calibrator according to claim 1, it is characterised in that the liquid weighing component (2) fixation kit (17) is additionally provided with, fixation kit (17) one end is fixed on high accuracy analysis balance (11), and the other end is solid It is scheduled on device pedestal (4).

5. high-precision pipettor automatic calibrator according to claim 1, it is characterised in that the fixation kit (17) For Z-shaped brandreth, high accuracy analysis balance (11) and device pedestal (4) are fixed by screw respectively.

6. high-precision pipettor automatic calibrator according to claim 1, it is characterised in that the suction nozzle box support (14) bottom is provided with suction nozzle box locating piece (18).

7. high-precision pipettor automatic calibrator according to claim 1, it is characterised in that the pipettor to be measured (9) four screws are fixed on the sliding block of down-feed screw (5) (10).

8. high-precision pipettor automatic calibrator according to claim 1, it is characterised in that the sliding block (10) and hang down Raw silk rings thick stick (5) is engaged by screw thread.