CN107242563A - A kind of fatigue resistant health food and preparation method thereof - Google Patents

Abstract

The invention discloses a kind of fatigue resistant health food and preparation method thereof, by maca, ginseng, gumbo extract, sealwort, ginkgo biloba extract, tribulus terrestris extraction, cinnamomum cassia extract, citrulling, L arginine.It is made by pulverizing and sieving → premixing → pelletizing drying → whole grain → total mixed → tabletting → coating → inner packing → outsourcing → storage.Safety of the invention, non-toxic, orally available, oral or instant, the healthcare function for the sleep that is significantly improved.

Description

A kind of anti-fatigue health food and preparation method thereof

technical field

The invention belongs to the field of health products, and in particular relates to an anti-fatigue health food and a preparation method thereof.

Background technique

Fatigue is a comprehensive physiological process involving many physiological and biochemical factors. It is a normal physiological phenomenon that must occur when the human body's mental or physical activities reach a certain stage. It not only marks the temporary decline of the original working ability of the body. , and may be a precursor to the development of the body to an injury state. People have given it many definitions when studying fatigue. In 1982, at the Fifth International Exercise Biochemistry Conference, fatigue was defined as: the body's physiological process cannot continue at a specific level of its function and/or cannot maintain a predetermined exercise strength.

Fatigue is a subjective sense of discomfort, mainly manifested as fatigue and burnout, which can be noticed during long-term labor, strenuous exercise or long-term mental stress. This is a normal physiological protective response, suggesting that the body should Rest to restore function and avoid further damage to the body. However, with the intensification of social competition and the acceleration of the pace of life, people often choose to work long hours regardless of fatigue. Being in a state of fatigue for a long time will damage the physical strength and fitness of the human body, and the body will be out of balance, making it difficult for people to engage in or complete some jobs that consume a lot of energy or have delicate and delicate movements, thereby reducing work efficiency; It makes people's skin loose and dull, showing signs of premature aging; long-term fatigue cannot be recovered, which makes the human immune system dysfunction, and even causes low immunity. Increase. In addition, long-term psychological stress, hormone disorders, and immune abnormalities are the main causes of chronic fatigue syndrome. Overload work, excessive pressure and irregular life schedule make people feel tired and burnout for a long time, which makes people in a sub-health state for a long time. About 70% of the people are in this state. Among them, middle-aged and elderly people have a high incidence.

A large number of research results confirm that fatigue may occur in such a wide range of regions from the cerebral cortex to the muscle subcellular structure. In 1980, Edwards proposed the concept of "nervous-muscular fatigue chain". Any link in the chain that is damaged or interrupted will cause a decrease in muscle strength and output power. According to the location of fatigue in the fatigue chain, it can be divided into two parts: central fatigue and external fatigue. Central fatigue refers to the fatigue that occurs in the area from the cerebral cortex→spinal cord→peripheral nerves; peripheral fatigue refers to the fatigue that occurs in the links below the nerve-muscle junction.

Based on this, it is necessary to find anti-fatigue substances to delay the occurrence of fatigue caused by long-term labor, strenuous exercise or excessive mental stress, or to restore the fatigue state to a normal state as soon as possible, in order to restore people's sub-health state to normal or treat fatigue syndrome. curative effect. The existing anti-fatigue health food is mainly based on traditional Chinese medicine, which is characterized by focusing on health preservation. Consumers need to take it for a long time, and the onset of effect is slow.

Therefore, it is of practical significance to provide a new anti-fatigue product.

Contents of the invention

In order to solve the above technical problems, one of the objectives of the present invention is to provide a health product for anti-fatigue and correction of sub-health state.

The second object of the present invention is to provide a preparation method of a health product for anti-fatigue and correction of sub-health state.

One of purpose of the present invention is achieved like this:

An anti-fatigue health food, comprising the following components in parts by weight:

The above-mentioned health food is also added with pharmaceutical excipients.

The health food mentioned above is oral liquid, drink, tablet, capsule, spray or transdermal absorber. The way of administration of this health food is oral or external application. The health food is taken 1-2 times a day, 100-2000 mg/kg body weight each time.

Two of the object of the present invention is achieved in this way:

A preparation method of an anti-fatigue health food, which is carried out in the following steps: crushing and screening of raw and auxiliary materials → premixing → granulation and drying → granulation → total mixing → tabletting → coating → inner packaging → outsourcing → storage.

The health food is used for correcting sub-health state or treating chronic fatigue syndrome; said sub-health state correction is increasing liver glycogen content, reducing whole blood lactic acid content or reducing plasma urea nitrogen content.

The following are the main ingredients of this health product.

L-arginine (Arginine)

Arginine is an amino acid containing two basic groups, an amino group and a guanidine group. Under physiological pH conditions, it is a basic amino acid (PH 10.5-12.0), with a molecular formula of C6H14N4O2, a molecular weight of 174.20, and a melting point of 223-224 ℃, white diamond-shaped crystals (precipitated from water, containing 2 molecules of crystal water) or monoclinic flaky crystals (no crystal water), odorless, bitter taste; easily soluble in water (0 ℃ water solubility is 83g/L, 50 ℃ Solubility in water is 400g/L), very slightly soluble in ethanol, insoluble in ether. There are two isomers in nature: D-arginine and L-arginine, which have important nutritional and physiological functions in animals It's L-arginine.

L-arginine is an important raw material for the synthesis of protein and creatine, and is a semi-essential amino acid in humans and animals. Arginine has important physiological, metabolic and nutritional functions. Almost all tissues in the body use Arg to synthesize cytoplasmic proteins and creatine. Nucleoprotein. Arginine also plays an important role in various physiological processes such as promoting protein synthesis in muscle, enhancing the body's immunity, cell division, wound recovery and secretion of hormones. Arginine is also a prerequisite for the synthesis of NO in the body. The increase of NO synthesis in the body has obvious effects on balancing blood, enhancing blood flow, improving heart and brain blood supply, enhancing blood vessel elasticity, and restoring blood vessel function. It is the strongest vasodilation factor, has the functions of anti-atherosclerosis and regulating blood pressure. The various biological functions of arginine have attracted widespread attention of nutrition and medical researchers, and thus become one of the hot spots of amino acid research. .

Citrulline

The latest discovery is that watermelon contains an important beneficial ingredient - citrulline. Amino acid is the basic unit of protein, and citrulline is a kind of amino acid. The content of citrulline in watermelon is higher than that of any food. People first extracted citrulline from watermelon, hence the name citrulline.

Citrulline is produced in the urea cycle from ornithine and carbamoyl phosphate, or as a by-product of NO from arginine catalyzed by nitric oxide synthase (NOS). First, arginine is oxidized to N-hydroxy-arginine, which is oxidized to citrulline and releases nitric oxide.

Moderate amounts of citrulline and various vitamins promote increased energy and maintain the immune system. Citrulline helps the body recover from fatigue and stress, and also maintains the body's pH balance.

To improve Jiankang's motor function, citrulline can react with ammonia in the human body to generate arginine and nitric oxide. Nitric oxide activates enzymes that lead to smooth muscle relaxation. It can also relax the blood vessels of the person, which is used to enhance the function and help the reproductive function.

Ginseng (Panax ginseng)

Ginseng, also known as Asian ginseng, is called "bangchui" in Northeast China. It is a fleshy root that can be used medicinally. Ginseng belongs to the Araliaceae family and grows mainly in East Asia, especially in cold regions. Ginseng is a common medicinal herb in Asia, and American ginseng is also commonly used in North and Central America.

The reason why ginseng is very rare and expensive is mainly related to its medicinal value. In the very early medical book "Shen Nong's Materia Medica", it is believed that ginseng has the effects of "replenishing the five internal organs, calming the spirit, calming the soul, relieving panic, eliminating evil spirits, making tomorrow happy and improving intelligence", and "long-term use can make life easier and prolong life". Li Shizhen also highly praised ginseng in "Compendium of Materia Medica", thinking that it can "cure all deficiencies in men and women".

More than 30 kinds of ginsenosides can be isolated from ginseng. In addition, ginseng also contains a variety of organic acids and esters, various vitamins, sterols, enzymes and more than 20 trace elements.

In daily life, ginseng and its preparation are often used for post-healing recovery, enhancing physical strength, regulating hormones, lowering blood sugar and controlling blood pressure, controlling liver index and liver function health care, etc.

Ginkgo biloba L.

Ginkgo is a plant with high medicinal value. The Ginkgo tree is an ancient tree species in my country. It is a magical tree of medicine. When the Jurassic dinosaurs took control of the earth more than 250 million years ago, Ginkgo was already the most prosperous one of the plants. Life on earth has gone through hundreds of billions of years of changes, especially after being covered by glaciers in the fourth century, only ginkgo still maintains its most primitive appearance, and is called a "living fossil" in the history of biological evolution. Its leaves, fruits, and seeds all have high medicinal value, its pharmacological effects are constantly being recognized, and the scope of clinical application is gradually expanding.

In recent years, the Chinese Academy of Sciences and many medical experts at home and abroad have found that more than 160 effective medicinal ingredients can be extracted from Ginkgo biloba leaves. Among them, there are 35 kinds of flavonoids; 17 kinds of amino acids and various trace elements.

Ginkgo biloba has many functions such as anti-aging, improving the body's immunity, and enhancing the body's resistance.

Cinnamon (Cortex Cinnamomi Cassiae)

Cinnamon is the dry bark and branch bark of Cinnamon and Daye Qinghua Gui of Lauraceae. Cinnamon is an evergreen tree, born in evergreen broad-leaved forests, but mostly cultivated. It is cultivated in tropical and subtropical regions in Fujian, Taiwan, Guangdong, Guangxi, Yunnan and other places in China, especially in Guangxi, where most of them are artificial pure forests.

Efficacy and function of cinnamon, cinnamon is spicy and sweet, hot in nature, and enters the kidney, spleen, and bladder meridian; Mild cold pulse, yang collapse, abdominal pain and diarrhea, cold colic, cold pain in the waist and knees, bleeding from the yin and gangrene, deficiency of yang floating above the heat and below the cold, etc.

Polygonatum sibiricum Delar.ex Redonte

Polygonatum Polygonatum is the rhizome of Polygonatum Polygonatum, Polygonatum Polyflora and Polygonatum Dianensis of Liliaceae. It belongs to the tonic traditional anti-aging health food. This taste is sweet, flat and non-toxic, and it belongs to the three meridians of the lung, spleen and kidney.

Polygonatum contains a variety of steroidal soaps, Polygonatum polysaccharides, digitalis sugar and a small amount of onion-like compounds, etc., which can prolong the survival period of silkworms, resist free radical damage, enhance immune function, regulate the level of the second messenger cyclic adenophosphate (cAMP), enhance Myocardial contractility, enhanced coronary blood flow, hypoglycemic, hypolipidemic, antibacterial, antiviral, antiradiation, antimutagenic, antifatigue, etc. It is a traditional Chinese medicine with good development prospects.

Tribulus terrestris L.

It is the fruit of Tribulus terrestris, an annual or perennial herb of the family Tribulus, mainly produced in Northeast China, North China, and Northwest China. Tribulus terrestris contains fatty oil, volatile oil, tannin, resin, sterol, potassium salt, trace alkaloid, saponin, etc. Its water extract and ethanol extract have antihypertensive effects on anesthetized animals; saponins from stems and leaves of Tribulus terrestris have obvious protective effects on mice subjected to high temperature, low temperature, hypoxia and swimming stress; Vitamin C (Vc) content in the body can also inhibit the depletion phenomenon caused by high-dose hydrocortisone, and play a protective role in the decline of Vc content caused by ACTH. After the removal of bilateral adrenal glands in mice, the hypoxia tolerance effect was no longer reproduced. These effects may be related to the adrenal cortex, and regulating its function from exhaustion may be one of the ways to achieve strength. Oral administration of Tribesta (a preparation made from the aerial parts of Tribulus terrestris) to male rats can significantly stimulate sperm formation, stimulate the activity of Sertolicell cells (Sertolicell), and increase libido; administration to female rats can promote estrus and improve fertility.

Okra (Hibiscus esculentus L.)

The aliases of okra include coffee yellow sunflower, yellow okra, hair eggplant and so on. Originated in Africa, it was introduced into my country from India in the early 20th century. Its edible part is the fruit pod, which is crisp, tender and juicy, smooth and not greasy, and has a unique fragrance, which is very popular among people.

The tender okra fruit contains a viscous liquid, arabinan, galactan, rhamnolan, protein, etc. It is often eaten to help digestion, enhance physical strength, protect the liver, and strengthen the stomach and intestines. Okra contains various nutrients such as iron, calcium and sugar, and has the effect of preventing anemia. Okra contains vitamin A and β-carotene, which are beneficial to the health of the retina and maintain vision; it is rich in trace elements such as zinc and selenium, which can enhance the body's anti-cancer and anti-cancer. The mucin in okra can inhibit the absorption of sugar, so it has been used as a folk prescription for treating diabetes in recent years. Okra also contains special medicinal ingredients, which can strengthen the kidney and tonify deficiency, and have an auxiliary therapeutic effect on male organic diseases. It enjoys the reputation of "plant Viagra".

Maca

Maca (Lepidium meyemii Walp) is an annual or biennial herb of the genus Brassicaceae. It is native to the Andes in central Peru at an altitude of 4000m or more. Because Maca is resistant to low temperature and strong wind, it can grow under harsh ecological conditions and has become one of the main local crops. The main edible part is the root. The diameter of the underground enlarged storage root is 2-5cm. The surface color is mostly yellow or purple, and the flesh is white or light yellow. It has a pungent smell and a special salty butterscotch smell. At present, the high-yield areas of maca (mainly cultivated products) are still in the Carhuamayo and Junin regions of the Andes. The situation of introduction to other regions of Peru or foreign countries such as Germany and the Czech Republic is not good, and satisfactory results have not been obtained due to dysplasia. Maca is suitable for growing in areas with an altitude of 4000-4500m in high cold, strong wind and high sunshine.

Maca (Maca) is a plant that can be used both as medicine and food. It is specially produced in Peru, South America. The local Incas used it as a tonic and called it Peruvian ginseng. In places such as Peru in South America, maca root can be eaten fresh and fried with meat or other vegetables, and cooked in water or milk after drying. For thousands of years, maca root has been used as food and herbal medicine to enhance Energy, anti-fatigue, improve sexual function, increase fertility, treat menopausal syndrome in women, etc.

experiment

Weight-bearing swimming is an important index to evaluate the body's exercise fatigue, and the length of swimming time can reflect the degree of animal exercise fatigue. Glycogen stored in the body is the main energy source for mice to exercise, and the body needs to consume a large amount of glycogen during strenuous exercise, so glycogen storage is also an important indicator for evaluating the body's resistance to fatigue. Lactic acid plays an important role in the energy supply system of the body. It is the final product of glycolysis energy supply and an important oxygen metabolism substrate in the aerobic metabolism energy supply system, and lactic acid can be used in the liver through glucose neogenesis converted to glucose for energy. However, if excessive lactic acid is produced in the body, the H+ concentration in the muscle will increase and the pH will decrease, which will cause a series of biochemical changes, which will cause fatigue. Studies have shown that the increase of urea nitrogen (BUN) is negatively correlated with the body's ability to adapt to load, and the body's BUN content increases with the increase of exercise load, so BUN is an ideal and sensitive fatigue indicator.

1 Materials and methods

1.1 Sample:

1.2 Experimental animals: five-month-old middle-aged male ICR mice; eight-month-old old male C57 mice.

1.3 Instruments and reagents: swimming box, lead wire, full-wavelength microplate reader, constant temperature water bath, centrifuge, liver glycogen detection kit (Nanjing Jiancheng, product number A043), lactic acid detection kit (Nanjing Jiancheng, product number A019-1) , Burea Nitrogen Kit (Nanjing Jiancheng, Item No. C013-1).

1.4 Experimental method:

A. The first batch: 60 male five-month-old middle-aged ICR mice were randomly divided into 4 groups, 15 in each group. The low-dose group received 400 mg/kg of drugs by intraperitoneal injection, the middle-dose group received 800 mg/kg of drugs by intraperitoneal injection, and the high-dose group received 1600 mg/kg of drugs by intraperitoneal injection. The control group was given the same volume of normal saline. Administer once a day from 10:00 am to 11:00 am for 7 consecutive days.

B. The second batch: 100 male eight-month old C57 mice were randomly divided into 4 groups, 20 in each group. The medicine of 400mg/kg was administered orally in the small dose group, the medicine of 800mg/kg was administered orally in the middle dose, and the medicine of 1600mg/kg was administered in large dose. The control group was given the same volume of water. Administer once a day from 10:00 am to 11:00 am for 7 consecutive days.

C. The third batch: 80 male five-month-old middle-aged ICR mice were randomly divided into 4 groups, 20 in each group. The control group was fed for 14 days under normal conditions, and the remaining three groups were subjected to chronic fatigue syndrome modeling: they were restrained from 9:00 am to 11:00 am every day for one hour each time, and the mice were put into water at a depth of 35 cm and a water temperature of Compulsory swimming in a swimming box at 21±1°C for 1 hour, and continuous modeling for 14 days. Begin to administer from the 15th day, the medicine of 400mg/kg is gavaged by stomach in a small dose, and the medicine of 1600mg/kg is gavaged by a large dose. The control group was given the same volume of water. Administer once a day from 10:00 am to 11:00 am for 7 consecutive days.

1.4.1 Weight-bearing swimming test

30 minutes after the last administration of health food, a lead wire with a weight of 4% of its body weight was given to the tail of the mice. The mice were put into a swimming box with a water depth of 35cm and a water temperature of 21±1°C to swim until exhausted. The criterion for judging exhaustion was that the head of the mouse did not surface within 10 seconds after being submerged in the water, and the exhaustion time was recorded.

1.4.2 Determination of liver glycogen

After swimming with weight, the mice rested for 10 minutes, and the mice were sacrificed after the eyeballs were removed for blood collection. The liver was taken out, rinsed with normal saline and blotted dry with filter paper, and 50 mg of the liver was accurately weighed. Hepatic glycogen was measured according to the kit instructions.

1.4.3 Determination of lactic acid in whole blood

After swimming with weight, the mice rested for 10 minutes, their eyeballs were removed, blood was collected, and whole blood lactic acid was measured according to the kit instructions.

1.4.4 Determination of plasma indicators

After swimming with weight, the mice rested for 10 minutes, blood was collected from the eyeballs, anticoagulated with EDTA sodium salt, centrifuged at 3500 rpm for 10 minutes, and the plasma was separated, and the plasma urea nitrogen was measured according to the kit instructions.

2 results

2.1 Determination of weight-bearing swimming time of mice

Figure 1A, Figure 1B, and Figure 1C show the effects of anti-fatigue health food on the weight-bearing swimming time of mice. Figure 1A shows the results of the first batch of intraperitoneal injection experiments; Figure 1B shows the results of the second batch of oral experiments, * indicates p<0.05. Compared with the control group, C is the result of the third batch of oral anti-chronic fatigue syndrome experiments. It can be seen from Figure 1A that intraperitoneal injection of this health food has a tendency to prolong the weight-bearing swimming time of mice, and this trend is dose-dependent; it can be seen from Figure 1B that compared with the control group, after oral administration of this health food The weight-bearing swimming time of the mice was prolonged in a dose-dependent manner, and the analysis of variance showed that there was a significant difference between the medium and high dose groups and the control group (p<0.05). It can be seen from Figure 1C that compared with the control group, the weight-bearing swimming time of the mice in the model group was significantly reduced, and the weight-bearing swimming time of the mice after oral administration of the health food was significantly prolonged compared with the model group.

2.2 Determination of mouse liver glycogen

Figure 2A, Figure 2B, and Figure 2C show the effects of anti-fatigue health food on mice liver glycogen storage, wherein Figure 2A is the first batch of intraperitoneal injection experiment results, * means p<0.05, compared with the control group; Figure 2B is The results of the second batch of oral experiments, * indicates p<0.05, compared with the control group; Figure 2C is the results of the third batch of oral anti-chronic fatigue syndrome experiments, * indicates p<0.05, compared with the control group. It can be seen from Figure 2 that, compared with the control group, no matter whether the health food is given by injection or orally, the liver glycogen content of the mice is significantly increased. reached a significant level (Fig. 2A), and the difference between the oral administration medium and high-dose groups and the control group reached a significant level, which was statistically significant (p<0.05) (Fig. 2B). In the anti-chronic fatigue syndrome model experiment, oral administration The difference between the small and medium dose groups and the model group reached a significant level (Fig. 2C), which was statistically significant (p<0.05).

2.3 Determination of lactic acid in whole blood of mice

Figure 3A, Figure 3B, Figure 3C show the effect of anti-fatigue health food on the level of lactic acid in whole blood of mice. Among them, Figure 3A is the result of the first batch of intraperitoneal injection experiments, * indicates p<0.05, compared with the control group; Figure 3B is the result of the second batch of oral administration, C is the third batch of oral anti-chronic fatigue syndrome experiment results, ** indicates p<0.01, compared with the control group. It can be seen from Figure 3A that after injection of this health food, compared with the control group, the whole blood lactic acid content decreased significantly, and the middle dose and high dose were the most significant (p<0.05); it can be seen from Figure 3B that compared with the control group, In comparison, the whole blood lactic acid content decreased after oral administration of this health food, but only the high dose group had statistical significance (p<0.05). It can be seen from Figure 3C that compared with the model group, the whole blood lactic acid content of the mice in the high-dose group after oral administration of the health food was significantly prolonged, which was statistically significant (p<0.01).

2.4 Determination of plasma urea nitrogen content in mice

Fig. 4A, Fig. 4B, Fig. 4C show the effect of anti-fatigue health food on the plasma urea nitrogen level of mice. Wherein Fig. 4A is the result of the first batch of intraperitoneal injection experiments, * means p<0.05, compared with the control group; Fig. 4B is the result of the second batch of oral experiments, * means p<0.05, compared with the control group; Fig. 4C is the Three batches of oral anti-chronic fatigue syndrome experimental results. It can be seen from Figure 4 that the plasma urea nitrogen content of each dose group of the health food decreased compared with the control group. Through analysis of variance, injection administration (Fig. 4A) middle and large dose groups had a high degree of significance with the control group, oral administration (Fig. 4B) high dose group had a high degree of significance with the control group (p<0.05), oral administration of anti Compared with the model group, chronic fatigue syndrome (Figure 4C) high-dose group had a downward trend, which was statistically significant (p<0.05).

Tests have shown that intraperitoneal injection of this anti-fatigue health food of the present invention has a tendency to prolong the weight-bearing swimming time of mice, and this trend is dose-dependent (Fig. 1A); After the anti-fatigue health food mixture, the weight-bearing swimming time of the mice was prolonged in a dose-dependent manner. According to variance analysis, there was a significant difference between each dose group and the control group (p<0.05) (Figure 1B). Compared with the control group, the weight-bearing swimming time of the mice in the model group was significantly decreased, and the weight-bearing swimming time of the mice after oral administration of the anti-fatigue health food was significantly prolonged compared with the model group ( FIG. 1C ).

Compared with the control group, whether the anti-fatigue health food was administered orally, the liver glycogen content of the mice was significantly increased, and the difference between the small, medium and high-dose injection groups and the control group reached a significant level (Fig. 2A), the difference between the oral administration medium and high dose groups and the control group reached a significant level (Figure 2B), which was statistically significant (p<0.05). In the anti-chronic fatigue syndrome model experiment, oral administration of large and small doses The difference between the model group and the model group reached a significant level (Fig. 2C), which was statistically significant (p<0.05).

After injecting the anti-fatigue health food, compared with the control group, the whole blood lactic acid content decreased significantly, especially in the medium dose and high dose (p<0.05) (Fig. 3A); compared with the control group, the oral administration of the anti-fatigue health food After treatment, the lactate content in whole blood decreased, but only the high-dose group had statistical significance (p<0.05) (Fig. 3B). Compared with the model group, after oral administration of anti-fatigue health food, the lactic acid content in the whole blood of the mice was significantly prolonged, which was statistically significant (p<0.01) (Figure 3C).

Compared with the control group, the plasma urea nitrogen content of each dose group of the anti-fatigue health food of the present invention has decreased. There is a high significance (p<0.05) (p<0.05) between the injection administration group and the control group through analysis of variance, and the oral administration high-dose group and the control group have a high significance, and the oral administration is anti-chronic fatigue syndrome (Fig. 4C) Compared with the model group, the high-dose group had a downward trend, which was statistically significant (p<0.05) (Fig. 4C).

Based on the above experimental results, the anti-fatigue health food provided by the present invention can significantly prolong the weight-bearing swimming time of mice, improve liver glycogen reserves of fatigue mice, and reduce the content of lactic acid and urea nitrogen in fatigue mice, suggesting that the present invention has obvious anti-fatigue effects. Fatigue effect.

The anti-fatigue syndrome treatment of this anti-fatigue health food obtains a similar effect.

According to the anti-fatigue function test method of health products, two of the three experiments were prolonging sleep time of pentobarbital sodium (or barbital sodium) subthreshold dose hypnosis test and pentobarbital sodium sleep latency test. If the item is positive, and there is no obvious direct sleep effect, it can be judged that the anti-fatigue function of the tested sample is positive in animal experiments. The above experimental results show that the health food has anti-fatigue function.

Animal experiments of the present invention prove that the health food has obvious improvement effect on fatigue, and has the advantages of non-toxicity, safety, oral administration, edible and the like.

The present invention finds that the health food is positive in the anti-fatigue function test method experiment, and can correct the sub-health state or treat chronic fatigue syndrome; the correction of the sub-health state is to increase liver glycogen content, reduce whole blood lactic acid content or reduce Plasma urea nitrogen content. The results of animal experiments show that it can prolong the weight-bearing swimming time of middle-aged and old mice, increase liver glycogen storage, reduce lactic acid and urea nitrogen content, have the advantages of small dosage, safety, injection and oral administration, etc., and have great health care and clinical application value. The anti-fatigue function provided by the invention can be applied to the preparation of anti-fatigue health products or food.

The specific research on the anti-fatigue of this health food has practical significance and application value for confirming its anti-fatigue and its health care function.

The advantages of the present invention are:

1. Safe, non-toxic and can be taken orally.

2. Made a new exploration of the function of health food, made a good supplement on the basis of its original function, and found its anti-fatigue effect.

3. This health food is convenient to take orally or eat, and has obvious anti-fatigue health care function. The recommended human dosage is about 25 grams (60kg.BW) of maca per day, and no more than 3 grams (60kg.BW) of ginseng per day.

Description of drawings

Fig. 1A is the histogram of the first batch of intraperitoneal injection experiment results of the health food of the present invention on the weight-bearing swimming time of mice;

Fig. 1B is a histogram of the results of the second batch of oral experiments on the weight-bearing swimming time of mice by the health food of the present invention;

Fig. 1C is a histogram of the third batch of oral anti-chronic fatigue syndrome experimental results of the health food of the present invention on the weight-bearing swimming time of mice;

Fig. 2A is a histogram of the first batch of intraperitoneal injection experiment results of the health food of the present invention on mouse liver glycogen reserve;

Fig. 2B is a histogram of the results of the second batch of oral administration of the health food of the present invention on mouse liver glycogen reserve;

Fig. 2C is a histogram of the third batch of oral anti-chronic fatigue syndrome experimental results of the health food of the present invention on mouse liver glycogen reserve;

Fig. 3A is a histogram of the results of the first batch of intraperitoneal injection experiments on the whole blood lactic acid level of mice by the health food of the present invention;

Fig. 3B is a histogram of the results of the second batch of oral experiments on the whole blood lactic acid level of mice by the health food of the present invention;

Fig. 3C is a histogram of the third batch of oral anti-chronic fatigue syndrome experimental results of the health food of the present invention on the whole blood lactic acid level of mice;

Fig. 4A is a histogram of the first batch of intraperitoneal injection experiment results of the health food of the present invention on the plasma urea nitrogen level in mice;

Fig. 4B is a histogram of the results of the second batch of oral experiments on the level of blood urea nitrogen in mice by the health food of the present invention;

Fig. 4C is a histogram of the third batch of oral anti-chronic fatigue syndrome test results of the health food of the present invention on the plasma urea nitrogen level of mice.

detailed description

The present invention will be further described below in conjunction with embodiment.

Example 1:

An anti-fatigue health food, the following components are prepared in parts by weight:

Maca: 30 parts;

Ginseng: 12 parts;

Okra extract: 5 parts; (okra extract: light yellow powder, extracted after mixing and crushing the roots, leaves, flowers and seeds of okra. Main extraction process: washing with water → adding boiled water to pulverize → filtering Residue → concentrated and dried. The concentration of the active ingredient in the extract is 10%).

Polygonatum: 4 parts;

Ginkgo biloba extract: 1 part; (Ginkgo biloba extract: light brown to tan powder. The active ingredient extract is extracted from the leaves of Ginkgo biloba L. with an appropriate solvent. The main production process It is: Ginkgo biloba → pretreatment such as washing with water → alcohol extraction → recovery and concentration → refining → spray drying. The concentration of the active ingredient in the extract is 20%).

Tribulus terrestris extract: 2 parts; (Tribulus terrestris extract: brown-yellow powder, the main production process is: Tribulus terrestris raw material→crushing→alcohol extraction→concentration→extraction→drying. Saponin content is 40%).

Cinnamon extract: 3 parts; (cinnamon extract: yellow-brown powder, mainly obtained from the bark of Lauraceae Cinnamomum cassia Presl. The main production process is: material selection → decoction → percolation → reflux → Distillation → Precipitation → Standing → Filtration → Concentration → Drying. The active ingredient content of the extract is 15%).

Citrulline: 4 servings;

L-arginine 8 parts

Add 2 parts of pharmaceutical excipients microcrystalline cellulose and 1 part of pharmaceutical excipients magnesium stearate to make tablets.

Then crush and sieve the raw materials → premixing → granulation and drying → granulation → total mixing → tabletting → coating → inner packaging → outsourcing → storage.

According to the dosage of 100-2000mg/kg body weight each time, 1-2 times a day, it can also be produced as oral liquid, beverage, capsule, spray or transdermal absorption agent.

Example 2:

An anti-fatigue health food, the following components are prepared in parts by weight:

Maca: 35 parts;

Ginseng: 16 parts;

Okra extract: 12 parts; (okra extract: light yellow powder, extracted after mixing and pulverizing the roots, leaves, flowers and seeds of okra. Main extraction process: washing with water → adding boiling water to pulverize → filtering Residue → concentrated and dried. The concentration of the active ingredient in the extract is 10%).

Polygonatum: 10 parts;

Ginkgo biloba extract: 3 parts; (Ginkgo biloba extract: light brown to tan powder. The active ingredient extract is extracted from the leaves of Ginkgo biloba L. with an appropriate solvent. The main production process It is: Ginkgo biloba leaves → pre-treatment such as washing with water → alcohol extraction → recovery and concentration → refining → spray drying. The concentration of active ingredients in the extract is 20%).

Tribulus terrestris extract: 6 parts; Tribulus terrestris extract: 2 parts; (Tribulus terrestris extract: brown yellow powder, the main production process is: Tribulus terrestris raw material→crushing→alcohol extraction→concentration→extraction→drying. Saponin content is 40%).

Cinnamon extract: 7 parts; (cinnamon extract: yellow-brown powder, mainly obtained from the bark of Lauraceae Cinnamomum cassia Presl. The main production process is: material selection → decoction → percolation → reflux → Distillation → Precipitation → Standing → Filtration → Concentration → Drying. The active ingredient content of the extract is 15%).

Citrulline: 9 servings;

L-arginine 10 parts

Add 2 parts of pharmaceutical excipients microcrystalline cellulose and 1 part of pharmaceutical excipients magnesium stearate to make tablets.

Then crush and sieve the raw materials → premixing → granulation and drying → granulation → total mixing → tabletting → coating → inner packaging → outsourcing → storage.

According to the dosage of 100-2000mg/kg body weight each time, 1-2 times a day, it can also be produced as oral liquid, beverage, capsule, spray or transdermal absorption agent.

Example 3:

An anti-fatigue health food, the following components are prepared in parts by weight:

Maca: 32 parts;

Ginseng: 14 parts;

Okra extract: 10 parts; (okra extract: light yellow powder, extracted after mixing and crushing the roots, leaves, flowers and seeds of okra. Main extraction process: washing with water → adding boiling water to pulverize → filtering Residue → concentrated and dried. The concentration of the active ingredient in the extract is 10%).

Polygonatum: 8 parts;

Ginkgo biloba extract: 2 parts; (Ginkgo biloba extract: light brown to tan powder. The active ingredient extract is extracted from the leaves of Ginkgo biloba L. with an appropriate solvent. The main production process It is: Ginkgo biloba → pretreatment such as washing with water → alcohol extraction → recovery and concentration → refining → spray drying. The concentration of the active ingredient in the extract is 20%).

Tribulus terrestris extract: 5 parts; (Tribulus terrestris extract: brown-yellow powder, the main production process is: Tribulus terrestris raw material→crushing→alcohol extraction→concentration→extraction→drying. Saponin content is 40%).

Cinnamon extract: 5 parts; (cinnamon extract: yellow-brown powder, mainly obtained from the bark of Lauraceae Cinnamomum cassia Presl. The main production process is: material selection → decoction → percolation → reflux → Distillation → Precipitation → Standing → Filtration → Concentration → Drying. The active ingredient content of the extract is 15%).

Citrulline: 6 servings;

L-arginine 9 parts

Add 2 parts of pharmaceutical excipients microcrystalline cellulose and 1 part of pharmaceutical excipients magnesium stearate to make tablets.

Then crush and sieve the raw materials → premixing → granulation and drying → granulation → total mixing → tabletting → coating → inner packaging → outsourcing → storage.

According to the dosage of 100-2000mg/kg body weight each time, 1-2 times a day, it can also be produced as oral liquid, beverage, capsule, spray or transdermal absorption agent.

Finally, it should be noted that the above description is only a preferred embodiment of the present invention, and those of ordinary skill in the art can make a variety of similar implementations under the inspiration of the present invention without violating the purpose and claims of the present invention. It means that such transformations all fall within the protection scope of the present invention.

Claims (4)

1. An anti-fatigue health food, comprising the following components in parts by weight: 2. A kind of anti-fatigue health food according to claim 1, characterized in that: said health food is also added with pharmaceutical excipients. 3. The anti-fatigue health food according to claim 1, characterized in that: the health food is oral liquid, beverage, tablet, capsule, spray or transdermal absorbent. 4. A preparation method of an anti-fatigue health food as claimed in claim 1-2, which is carried out as follows: raw and auxiliary materials are crushed and sieved → premixed → granulated and dried → granulated → total blended → tabletted → packaged clothing→inner packaging→outsourcing→warehousing.