CN109750077A - The Preparation method and use of Doby Asia cockroach symbiotic effects activity secondary metabolite - Google Patents
The Preparation method and use of Doby Asia cockroach symbiotic effects activity secondary metabolite Download PDFInfo
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Abstract
本发明公开了杜比亚蟑螂共生真菌活性次生代谢产物的制备方法及用途，该方法包含：将从杜比亚蟑螂上分离得到的共生真菌采用PDB培养基进行发酵培养，抽滤，将菌丝和菌液分离，菌液直接采用乙酸乙酯萃取，减压浓缩，得到菌液活性次生代谢产物；具有活性次生代谢产物的杜比亚蟑螂共生真菌包含：Penicillium sp.Aspergillus sp.和Clonostachys sp.其中，所述的Penicillium sp.具有抗菌和抗氧化活性；其中，所述的Aspergillus sp.和Clonostachys sp.具有抗菌活性。本发明的方法能够通过发酵培养提取共生真菌的次生代谢产物，并测定其抗菌和抗氧化活性，以期为杜比亚蟑螂及其共生真菌的综合开发与利用提供重要的理论依据。The invention discloses a preparation method and application of active secondary metabolites of Dubia cockroach symbiotic fungi. The method comprises: fermenting and culturing symbiotic fungi isolated from Dubia cockroaches using PDB medium, suction filtration, and filtering the bacteria. The filaments and the bacterial liquid are separated, the bacterial liquid is directly extracted with ethyl acetate, and concentrated under reduced pressure to obtain the active secondary metabolites of the bacterial liquid; the Dubia cockroach symbiotic fungi with active secondary metabolites include: Penicillium sp.Aspergillus sp. and Clonostachys sp. wherein the Penicillium sp. has antibacterial and antioxidant activity; wherein, the Aspergillus sp. and Clonostachys sp. have antibacterial activity. The method of the invention can extract the secondary metabolites of the symbiotic fungi through fermentation and culture, and measure their antibacterial and antioxidant activities, so as to provide an important theoretical basis for the comprehensive development and utilization of Dubia cockroach and its symbiotic fungi.
Description
Technical field
The present invention relates to a kind of fungal component secondary metabolites, and in particular to Doby Asia cockroach symbiotic effects activity secondary generation
Thank to the Preparation method and use of product.
Background technique
Doby Asia cockroach (Blaptica dubia) also known as big Lian, Du Biya cockroach of Du Biya belong to Blattaria
(Blattaria), Blattidae (Blattidae), green Lian belong to (Blaptica) soft viviparous insect, are distributed in Central America, South America,
Belong to sanitary insect pest.The intracorporal rich in protein of Doby Asia cockroach, is now chiefly used in the living body of reptile and amphibian
Feed is one of biggish resource insect of potentiality to be exploited.Chitin, antibacterial peptide and the chitosan etc. that Doby Asia cockroach generates have been used
In production Medicines and Health Product, cosmetics etc..
With the continuous improvement to microbe research method in insect bodies, a large amount of symbiotic effects are constantly by people in vivo
It is known.Research finds that microorganism participates in many aspects that insect lives in insect bodies, and physiology and evolution including insect are assisted
Enteron aisle digests food, improves host's nutrition, is conducive to communication in inter-species kind, resists entering for predator, pathogen and helminth
It invades.
Insect symbiotic effects and its secondary metabolite type of generation be abundant, structure novel, resistance are preferable, is current state
The emphasis of inside and outside research.Sorres etc. (Phytochemistry, 2018,151:69-77) isolates symbiosis from the nest of termite
Fungi Neonectria discophora SNB-CN63, the 6 ilicicolin derivatives obtained after fermented and cultured are shown
Preferable antimicrobial acivity.(Indian Journal of Microbiology, 2018,58 (2): 146- such as Barretto
158) it is separated to staphylococcus flavine from the commensal gut bacterium Staphylococcusgallinarum of silkworm, the material exhibits
Good antibacterium, anti-oxidant and anticancer activity out.
The research about Doby Asia cockroach symbiotic effects is had not yet to see, therefore it is necessary to whether there is symbiotic effects for it
And the biological characteristics of symbiotic effects are studied.
Summary of the invention
The object of the present invention is to provide the Preparation method and use of Doby Asia cockroach symbiotic effects activity secondary metabolite,
The problem of this method solve there has been no the researchs to Doby Asia cockroach symbiotic effects at present can be extracted altogether by fermented and cultured
The secondary metabolite of raw fungi, and measure its antibacterial and antioxidant activity, to be Doby Asia cockroach and its symbiotic effects
Comprehensive exploitation and the theoretical foundation important using offer.
In order to achieve the above object, the present invention provides the preparations of Doby Asia cockroach symbiotic effects activity secondary metabolite
Method, this method include: will carry out fermentation training from the upper isolated symbiotic effects of Doby Asia cockroach using PDB culture medium bacterium
It supports, filters, mycelia and bacterium solution are separated, bacterium solution directlys adopt ethyl acetate extraction, and extract liquor is concentrated under reduced pressure, and it is living to obtain bacterium solution
Property secondary metabolite；The Doby Asia cockroach symbiotic effects of active secondary metabolite include: Penicillium sp.,
Aspergillus sp. and Clonostachys sp..
Wherein, the Penicillium sp. has antibacterial and antioxidant activity.
Wherein, Aspergillus sp. and the Clonostachys sp. has antibacterial activity.
Preferably, the in vitro organ of Doby Asia cockroach is flat in the PDA culture medium of the streptomycin sulphate containing 500 μ g/mL
In dark culture in 28 DEG C of constant incubators in plate, obtain symbiotic effects, from a small amount of mycelium inoculation of edge picking of each bacterium colony to
In new PDA culture medium, continuous purification is multiple, the symbiotic effects isolated and purified.
The present invention also provides the Doby Asia cockroach symbiotic effects activity secondary metabolisms that the preparation method described in one kind obtains
The Doby Asia cockroach symbiotic effects of the purposes of product, active secondary metabolite include: Penicillium sp.,
Aspergillus sp. and Clonostachys sp.；Wherein, the Penicillium sp. has antibacterial and anti-oxidant
Activity；Wherein, Aspergillus sp. and the Clonostachys sp. has antibacterial activity.
Preferably, the Penicillium sp., Aspergillus sp. and Clonostachys sp. are to large intestine
Bacillus, avenae subsp.citrull, tomato Streptomyces scabies, bacillus subtilis, ralstonia solanacearum, Agrobacterium tumefaciens and haemolysis grape ball
Bacterium has bacteriostasis.
The Preparation method and use of Doby Asia cockroach symbiotic effects activity secondary metabolite of the invention solves at present
The problem of there has been no researchs to Doby Asia cockroach symbiotic effects, has the advantage that
The present invention passes through time that fermented and cultured extracts symbiotic effects by separating, purifying and identify symbiotic effects therein
Raw metabolite, and measure its antibacterial and antioxidant activity, to for Doby Asia cockroach and its symbiotic effects comprehensive exploitation and
The important theoretical foundation of offer is provided.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
The separation of 1 Doby Asia cockroach symbiotic effects of experimental example
Living body Doby Asia cockroach is cleaned into 20min with clear water, is dried.First with 75% alcohol treatment 30s, then with 0.2% chlorine
Change mercury and handle 20min, then uses aseptic water washing 3 times, each 5min is finally placed on aseptic filter paper and dries.It is sub- to remove Doby
Wing, foot and the cephalothorax of cockroach are placed in PDA culture medium plate the (sulfuric acid containing 500 μ g/mL after splitting the abdomen of cockroach
Streptomysin), the dark culture in 28 DEG C of constant incubators.After symbiotic effects are grown, from a small amount of bacterium of edge picking of each bacterium colony
Silk is inoculated into new PDA culture medium, and continuous purification is multiple, until colonial morphology uniformity.It will isolate and purify to obtain
Doby Asia cockroach symbiotic effects number consecutively be Bdf-1~Bdf-5.Purified symbiotic effects are inoculated on the inclined-plane PDA,
4 DEG C of preservations, it is spare.
The identification of 2 Doby Asia cockroach symbiotic effects of experimental example
(1) Morphological Identification
By symbiotic effects strain inoculated on PDA plate, 28 DEG C of 5~10d of constant temperature incubation, observation, record colonial morphology are simultaneously
It takes pictures, and observation hypha form and production spore situation under an optical microscope, whether there is or not diaphragms and the size of spore etc. for record mycelia
Deng.
It has been observed that all bacterial strain mycelia being separated to are septate hypha, in regular culture conditions under optical microscopy
Under have spore generation.
(2) molecular biology identification
Bacterial strain (5d is grown on PDA plate) after purification is inoculated into PDB culture medium, in 28 DEG C of 150rpm oscillation trainings
6~7d is supported, decompression obtains its mycelia after filtering.Mycelia is fully ground to powdered with liquid nitrogen, using RNA isolation kit, (Shanghai is raw
Work DNA takes out extracts kit) extract its DNA.
Use fungi universal primer ITS4 (5 '-TCCTCCGCTTATTGATATGC-3 ') and ITS5 (5 '-
GGAAGTAAAAGTCGTAACAAGG-3 ') expand its ITS sequence.
PCR reaction system (50 μ L): 2 × Taq PCR MasterMix (containing dyestuff) 25 μ L, ITS4 (10 μm of ol/L) 1 μ L,
ITS5 (10 μm of ol/L) 1 μ L, DNA profiling (10ng/ μ L) 2 μ L；21 μ L of distilled water is mixed.
Amplification program: 94 DEG C of initial denaturation 3min；94 DEG C of denaturation 40s, 56 DEG C of annealing 40s, 72 DEG C of extension 1min 20s, totally 30
A circulation；Last 72 DEG C of extensions 10min, 4 DEG C of preservations.
Gained sequence carries out complementary splicing using DNAMAN software, and positive add with reverse primer complementary series both ends is drawn
Object sequence assembly is at complete sequence.
Resulting rDNA-ITS sequence will be expanded and carry out Blast on the website NCBI, carried out in GeneBank database
Homology search.Bdf-1 is Penicillium sp. (GenBank accession number: MH681591), Bdf-2 Aspergillus
Sp. (GenBank accession number: MH681592), Bdf-3 are Aspergillus sp. (GenBank accession number: MH681593),
Bdf-4 is Clonostachys sp. (GenBank accession number: MH681594), and Bdf-5 is Aspergillus sp.
(GenBank accession number: MH681595), all bacterial strains are known bacterial strain.
The identification table of the Doby Asia cockroach symbiotic effects of the invention of table 1
The preparation of 3 Doby Asia cockroach symbiotic effects activity secondary metabolite of experimental example
Fermented and cultured is carried out to the symbiotic effects being separated and identified using PDB culture medium, is respectively obtained by depressurizing to filter
Mycelia and bacterium solution.Mycelia is extracted 3 times, each 7d through ethyl acetate cold soaking, and bacterium solution directlys adopt ethyl acetate and extracts 3 times, respectively
Extracting solution and extract liquor are concentrated under reduced pressure, obtain mycelia and bacterium solution secondary metabolite, 4 DEG C save backup.
The measurement of 4 Doby Asia cockroach symbiotic effects activity secondary metabolite antibacterial activity of experimental example
Symbiotic effects secondary metabolite pair is measured using thin-layer chromatography (TLC)-thiazolyl blue (MTT)-bioautography
Inhibitory activity of the difference for examination bacterium.
For trying bacterium: Escherichia coli (Escherichia coli, G-), avenae subsp.citrull (Pseudomonas
Lachrymans, G-), tomato Streptomyces scabies (Xanthomonas vesicatoria, G-), bacillus subtilis (Bacillus
Subtilis, G+), ralstonia solanacearum (Ralstonia solanacearum, G-), Agrobacterium tumefaciens (Agrobacterium
Tumefaciens, G-) and staphylococcus haemolyticus (Staphylococcus haemolyticus, G+)。
By mycelia or bacterium solution secondary metabolite acetone solution, the capillary of 0.5mm diameter is used in thin layer chromatography board
Point sample, point sample amount are 5 μ L.Thin-layer chromatography is carried out as solvent using the mixed solution of methylene chloride and methanol, concentration is
The streptomycin sulphate of 0.2mg/mL is as positive control.Bacterium solution (45mL LB+5mL is added into the LB semisolid culturemedium of sterilizing
Bacterium solution), adjusting its concentration is about 108CFU/mL vibrates, uniformly.Bacterium solution is uniformly and quickly sprayed on silica gel plate with spray sample device,
After culture medium cooled and solidified, silica gel plate is placed in culture dish and places 4h in 4 DEG C of refrigerators, in favor of the expansion of antimicrobial component
It dissipates；Silica gel plate is placed in moisturizing culture at 28 DEG C again, takes out silica gel plate after 12h, the MTT of 0.5mg/mL is uniformly sprayed, waits for quietly several
Observable experimental result after minute.
Have at Antibacterial Constituents, antibacterial spot occurs due to being inhibited by active constituent for examination bacterium；No antibacterial is living
Property ingredient at, for try bacterium normal growth, MTT colour developing after show blue.By the mobility of antibacterial spot come in preliminary assessment sample
The polarity of antimicrobial compound, according to the size of antibacterial spot and how much come the bacteriostatic activity and quantity of preliminary assessment reactive compound,
It as shown in table 2 below, is Doby Asia cockroach symbiotic effects activity secondary metabolite antibacterial activity result table of the invention.
The Doby Asia cockroach symbiotic effects activity secondary metabolite antibacterial activity result table of the invention of table 2
Note: solvent is methylene chloride: methanol=20:1 (v/v), and color developing agent is 5%MTT solution." -- " shows without antibacterial
Spot；"+" shows the maximum gauge d=0-5mm of antibacterial spot, and " ++ " shows d=5-10mm, and " +++ " shows d >=10mm.
The result shows that 5 kinds of symbiotic effects bacterium solutions and mycelia extract show certain antibacterial activity, but same extraction
Object has a certain difference the different inhibitory activity for trying bacterium.Wherein, Bdf-1 mycelia extract to avenae subsp.citrull not
Inhibitory activity is shown, and Bdf-3 mycelia extract does not show inhibitory activity to bacillus subtilis.All bacterial strain bacterium solutions mention
It takes Rf value (mobility of antibacterial spot) range of the antibacterial spot of object to be significantly greater than mycelia extract, illustrates in bacterium solution containing more anti-
Bacterium reactive compound.
Rf value is mainly related with the polarity of compound, and Rf value is smaller, and compound polarity is bigger, Bdf-1, Bdf-3 and Bdf-4
Bacterium solution extract Rf value is distributed between 0.0~0.5, illustrates the secondary metabolism in the bacterium solution of three plants of bacterium with antibacterial activity
Its polarity of product is medium bigger than normal.Bdf-1 and Bdf-3 mycelia extract Rf value is mainly distributed between 0.35-0.60, illustrates two
Substance in the mycelia of bacterium with antibacterial activity is mainly moderately polar secondary metabolite, and Bdf-4 mycelia extract Rf
Value is mainly distributed between 0.0-0.12, illustrates that the polarity of antibacterial substance is bigger than normal.The suppression of Bdf-2 and Bdf-5 bacterium solution extract
Mycelia extract is completely covered in the Rf value range of bacterial plaque, illustrates there is the secondary metabolite of bacteriostatic activity more in bacterium solution extract
Horn of plenty.From the point of view of the maximum gauge of antibacterial spot, Bdf-4 and Bdf-5 bacterium solution extract to it is all for try bacterium antibacterial spots most
Major diameter is more than 10mm, and mycelia extract will be markedly less than bacterium solution, antibacterial spot maximum gauge generally between 5-10mm,
Middle Bdf-4 mycelia extract is greater than 10mm to the maximum gauge of avenae subsp.citrull and the antibacterial spot of Escherichia coli.Except cucumber angle
Outside pinta bacterium, Bdf-2 bacterium solution extract to other for try bacterium antibacterial spot maximum gauge also above 10mm, mycelia extract
It is weaker than bacterium solution extract.Bdf-1 and Bdf-3 will be weaker than Bdf-2, Bdf-4 and Bdf-5 to the different inhibitory activity for trying bacterium,
But certain antibacterial activity is also showed, the maximum gauge of antibacterial spot focuses mostly between 5-10mm.
The measurement of 5 Doby Asia cockroach symbiotic effects activity secondary metabolite antioxidant activity of embodiment
Using porous plate-DPPH determination of color symbiotic effects secondary metabolite to the clearance rate of DPPH, with IC50Value is come
Indicate the oxidation resistance of endogenetic fungus secondary metabolite.
Precision weighs DPPH 20.0mg and is dissolved in 100mL dehydrated alcohol, and oscillation shakes up, and makes its final concentration of 0.2mg/mL.
The initial concentration of positive control BHT and each extract is 10.0mg/mL, and it is 5mg/ that concentration is successively half-and-half then diluted to DMSO
The solution of mL~0.0390625mg/mL.It is molten that the DPPH dehydrated alcohol that 80 μ L concentration are 0.2mg/mL is added into 96 microwell plates
Liquid, is then added the testing sample solution or positive control solution of 20 μ L series of concentrations, and concussion shakes up.The water-bath at 37 DEG C
Light absorption value is measured under 30min, 517nm.Replace sample solution as blank control, 3 weights of each processing using 20 μ L DMSO solutions
It is multiple, median effective concentration (IC of the test sample to DPPH clearance rate50) calculation formula is as follows:
The data obtained carries out mapping analysis using Excel software, and test sample concentration takes logarithm (X), and clearance rate is converted into
Probit value (Y) tentatively acquires the regression equation (Y=aX+b) and IC of antioxidant activity50Value.
The antioxidant activity of 5 plants of Doby Asias cockroach symbiotic effects bacterium solutions and mycelia extract is determined using DPPH method, such as
It is Doby Asia cockroach symbiotic effects activity secondary metabolite antioxidant activity result table of the present invention shown in table 3.The result shows that
Bdf-4 and Bdf-5 bacterium solution and mycelia extract do not show any antioxidant activity under for examination concentration.Bdf-1, Bdf-2
Apparent antioxidant activity, IC are shown with the bacterium solution extract of Bdf-350Value be respectively 0.26 ± 0.01mg/mL, 2.20 ±
0.99mg/mL and 0.75 ± 0.16mg/mL；And the IC of mycelia extract50Value is all larger than 5mg/mL.Illustrate Bdf-1, Bdf-2 and
Secondary metabolite in Bdf-3 with antioxidant activity is distributed mainly in bacterium solution.
The Doby Asia cockroach symbiotic effects activity secondary metabolite antioxidant activity result table of the present invention of table 3
It is discussed in detail although the contents of the present invention have passed through above preferred embodiment, but it should be appreciated that above-mentioned
Description is not considered as limitation of the present invention.After those skilled in the art have read above content, for of the invention
A variety of modifications and substitutions all will be apparent.Therefore, protection scope of the present invention should be limited to the appended claims.
Claims (4)
1. the preparation method of Doby Asia cockroach symbiotic effects activity secondary metabolite, which is characterized in that this method includes:
Fermented and cultured will be carried out from the upper isolated symbiotic effects of Doby Asia cockroach using PDB culture medium bacterium, filtered, by mycelia
It is separated with bacterium solution, bacterium solution directlys adopt ethyl acetate extraction, is concentrated under reduced pressure, obtains bacteria solution active secondary metabolite；
The Doby Asia cockroach symbiotic effects of active secondary metabolite include: Penicillium sp., Aspergillus
Sp. with Clonostachys sp.；
Wherein, the Penicillium sp. has antibacterial and antioxidant activity；
Wherein, Aspergillus sp. and the Clonostachys sp. has antibacterial activity.
2. the preparation method of Doby Asia cockroach symbiotic effects activity secondary metabolite according to claim 1, feature
It is, the in vitro organ of Doby Asia cockroach is in the PDA culture medium plate of the streptomycin sulphate containing 500 μ g/mL in 28 DEG C
Dark culture in constant incubator obtains symbiotic effects, cultivates from a small amount of mycelium inoculation of edge picking of each bacterium colony to new PDA
On base, continuous purification is multiple, the symbiotic effects isolated and purified.
3. a kind of Doby Asia cockroach symbiotic effects activity secondary metabolism that preparation method according to claim 1 or 2 obtains
The purposes of product, which is characterized in that the Doby Asia cockroach symbiotic effects of active secondary metabolite include:
Penicillium sp., Aspergillus sp. and Clonostachys sp.；Wherein, the Penicillium sp.
With antibacterial and antioxidant activity；Wherein, Aspergillus sp. and the Clonostachys sp. is living with antibacterial
Property.
4. the preparation method of Doby Asia cockroach symbiotic effects activity secondary metabolite according to claim 3, feature
It is, the Penicillium sp., Aspergillus sp. and Clonostachys sp. are to Escherichia coli, cucumber angle
Pinta bacterium, tomato Streptomyces scabies, bacillus subtilis, ralstonia solanacearum, Agrobacterium tumefaciens and staphylococcus haemolyticus have antibacterial
Effect.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110283053A (en) * | 2019-07-08 | 2019-09-27 | 华南农业大学 | A rapid separation and preparation method of symbiotic fungal monomer compound and its application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110207604A1 (en) * | 2010-02-25 | 2011-08-25 | Marrone Bio Innovations | Isolated bacterial strain of the genus burkholderia and pesticidal metabolites therefrom |
| CN103787953A (en) * | 2014-01-17 | 2014-05-14 | 杭州维康科技有限公司 | Compound Aspochalasin V and preparation method and application thereof |
-
2019
- 2019-01-15 CN CN201910036819.6A patent/CN109750077A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110207604A1 (en) * | 2010-02-25 | 2011-08-25 | Marrone Bio Innovations | Isolated bacterial strain of the genus burkholderia and pesticidal metabolites therefrom |
| CN103261398A (en) * | 2010-02-25 | 2013-08-21 | 马罗内生物创新公司 | Isolated Bacterial Strains of Burkholderia and Their Pesticide Metabolites |
| CN103787953A (en) * | 2014-01-17 | 2014-05-14 | 杭州维康科技有限公司 | Compound Aspochalasin V and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| HAMID KASSIRI等: "Pathogenic Fungal Species Associated with Digestive System of Periplaneta americana (Blattaria: Blattidae) Trapped from Residential Dwellings in Ahvaz City, Southwestern Iran", 《J ARTHROPOD-BORNE DIS.》 * |
| 赵斌: "蜚蠊共生菌Aspergillus sp.次级代谢产物及其功能的研究", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110283053A (en) * | 2019-07-08 | 2019-09-27 | 华南农业大学 | A rapid separation and preparation method of symbiotic fungal monomer compound and its application |
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