CN109825486A - 用于进行同源重组的方法、宿主细胞和试剂盒 - Google Patents

用于进行同源重组的方法、宿主细胞和试剂盒 Download PDF

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Publication number
CN109825486A
CN109825486A CN201810875866.5A CN201810875866A CN109825486A CN 109825486 A CN109825486 A CN 109825486A CN 201810875866 A CN201810875866 A CN 201810875866A CN 109825486 A CN109825486 A CN 109825486A
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nucleic acid
rece
linear
red
acid molecule
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CN201810875866.5A
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English (en)
Chinese (zh)
Inventor
Y·张
J·付
F·斯图尔特
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Gene Bridges GmbH
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Gene Bridges GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/16—Exonucleases active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters (3.16)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201810875866.5A 2010-06-10 2011-06-10 用于进行同源重组的方法、宿主细胞和试剂盒 Pending CN109825486A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1009732.7A GB201009732D0 (en) 2010-06-10 2010-06-10 Direct cloning
GB1009732.7 2010-06-10
CN201180038908.6A CN103068995B (zh) 2010-06-10 2011-06-10 直接克隆

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201180038908.6A Division CN103068995B (zh) 2010-06-10 2011-06-10 直接克隆

Publications (1)

Publication Number Publication Date
CN109825486A true CN109825486A (zh) 2019-05-31

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
CN201810875866.5A Pending CN109825486A (zh) 2010-06-10 2011-06-10 用于进行同源重组的方法、宿主细胞和试剂盒
CN201180038908.6A Active CN103068995B (zh) 2010-06-10 2011-06-10 直接克隆

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201180038908.6A Active CN103068995B (zh) 2010-06-10 2011-06-10 直接克隆

Country Status (8)

Country Link
US (2) US10443051B2 (2)
EP (2) EP3138921A1 (2)
JP (2) JP5934196B2 (2)
CN (2) CN109825486A (2)
CA (1) CA2802167C (2)
GB (1) GB201009732D0 (2)
SG (1) SG186219A1 (2)
WO (1) WO2011154927A2 (2)

Cited By (1)

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CN115717152A (zh) * 2022-11-29 2023-02-28 江南大学 一种控制枯草芽孢杆菌基因组同源重组概率的方法

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GB201009732D0 (en) * 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
EP2612918A1 (en) * 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
CN102558309B (zh) * 2012-02-10 2014-09-17 浙江大学 一对转录激活子样效应因子核酸酶及其编码基因与应用
CN102627691B (zh) * 2012-04-05 2014-09-17 浙江大学 一对转录激活子样效应因子核酸酶及其编码基因与应用
SG11201406547YA (en) * 2012-04-25 2014-11-27 Regeneron Pharma Nuclease-mediated targeting with large targeting vectors
CN102702335B (zh) * 2012-05-23 2014-07-09 上海斯丹赛生物技术有限公司 重组转录激活子样效应因子、转录激活子样效应因子核酸酶及其编码基因及应用
GB2520903A (en) 2012-10-16 2015-06-03 Gene Bridges Gmbh Electrocompetent cells and preparation thereof
DK3456831T3 (da) 2013-04-16 2021-09-06 Regeneron Pharma Målrettet modifikation af rottegenom
KR102170502B1 (ko) 2013-12-11 2020-10-28 리제너론 파마슈티칼스 인코포레이티드 게놈의 표적화된 변형을 위한 방법 및 조성물
EP3083958B1 (en) * 2013-12-19 2019-04-17 Amyris, Inc. Methods for genomic integration
HRP20200529T1 (hr) 2014-06-06 2020-09-04 Regeneron Pharmaceuticals, Inc. Postupci i pripravci za modificiranje ciljnog lokusa
ES2781323T3 (es) 2014-06-23 2020-09-01 Regeneron Pharma Montaje de ADN mediado por nucleasas
SI3161128T1 (sl) 2014-06-26 2019-02-28 Regeneron Pharmaceuticals, Inc. Postopki in sestavki za ciljano genetsko spremembo in postopki uporabe
KR102531016B1 (ko) 2014-11-21 2023-05-10 리제너론 파마슈티칼스 인코포레이티드 쌍 형성된 가이드 rna를 사용하는 표적화된 유전자 변형을 위한 방법 및 조성물
CN105713885B (zh) * 2014-12-04 2019-06-11 清华大学 特异识别并修复β地中海贫血症beta-globin基因的嵌合核酸酶
ES2947714T3 (es) * 2014-12-19 2023-08-17 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida a través de múltiples direccionamientos en un solo paso
CN105734061B (zh) * 2016-03-25 2019-07-23 中国农业科学院生物技术研究所 从毕赤酵母中分离的鼠李糖诱导型启动子及其应用
EP3440213A1 (en) * 2016-04-06 2019-02-13 Gene Bridges GmbH Adenoviral vectors
CN106995813B (zh) * 2017-03-23 2020-06-16 山东大学 基因组大片段直接克隆和dna多分子组装新技术
CN109385417A (zh) * 2017-08-03 2019-02-26 华东理工大学 体内dna无缝组装方法
KR102931179B1 (ko) * 2019-04-05 2026-02-25 다니스코 유에스 인크. 선형 재조합 dna 작제물 및 이의 조성물을 이용하여 공여 dna 서열을 바실러스 게놈 내에 통합시키기 위한 방법
CN110184287B (zh) * 2019-05-24 2024-01-30 华南农业大学 一种制备重组病毒的方法及其应用
CN114630910A (zh) 2019-06-25 2022-06-14 伊纳瑞农业技术有限公司 改善的同源依赖修复基因组编辑
EP4715055A1 (en) 2024-09-19 2026-03-25 European Molecular Biology Laboratory Method for high efficiency bacterial in vivo or in vitro cloning

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Publication number Priority date Publication date Assignee Title
CN115717152A (zh) * 2022-11-29 2023-02-28 江南大学 一种控制枯草芽孢杆菌基因组同源重组概率的方法

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Publication number Publication date
EP3138921A1 (en) 2017-03-08
US12195732B2 (en) 2025-01-14
US20130210681A1 (en) 2013-08-15
CA2802167A1 (en) 2011-12-15
WO2011154927A9 (en) 2013-01-24
WO2011154927A2 (en) 2011-12-15
SG186219A1 (en) 2013-01-30
CN103068995B (zh) 2020-10-16
JP2016135158A (ja) 2016-07-28
JP2013530689A (ja) 2013-08-01
CA2802167C (en) 2023-03-07
US20200283759A1 (en) 2020-09-10
US10443051B2 (en) 2019-10-15
WO2011154927A3 (en) 2012-02-16
GB201009732D0 (en) 2010-07-21
EP2580338A2 (en) 2013-04-17
JP5934196B2 (ja) 2016-06-15
EP2580338B1 (en) 2016-10-05
CN103068995A (zh) 2013-04-24

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