CN110012694A - Method for promoting rapid germination of kalopanax septemlobus seeds - Google Patents
Method for promoting rapid germination of kalopanax septemlobus seeds Download PDFInfo
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- 230000035784 germination Effects 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 36
- 230000001737 promoting effect Effects 0.000 title claims abstract description 14
- 241000319057 Kalopanax septemlobus Species 0.000 title 1
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 38
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000003448 gibberellin Substances 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 241000723422 Catalpa Species 0.000 claims abstract description 26
- 241000196324 Embryophyta Species 0.000 claims abstract description 22
- 239000003415 peat Substances 0.000 claims abstract description 22
- 238000002791 soaking Methods 0.000 claims abstract description 10
- 238000010438 heat treatment Methods 0.000 claims abstract description 7
- 239000000758 substrate Substances 0.000 claims abstract description 4
- 239000004576 sand Substances 0.000 claims description 25
- 239000011159 matrix material Substances 0.000 claims description 11
- 238000009331 sowing Methods 0.000 claims description 8
- 235000019362 perlite Nutrition 0.000 claims description 7
- 239000010451 perlite Substances 0.000 claims description 7
- 238000004140 cleaning Methods 0.000 abstract description 4
- 238000005498 polishing Methods 0.000 abstract description 2
- 238000005406 washing Methods 0.000 abstract 1
- 238000011160 research Methods 0.000 description 11
- 235000013399 edible fruits Nutrition 0.000 description 9
- 239000002775 capsule Substances 0.000 description 8
- 238000013401 experimental design Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 239000012535 impurity Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000007226 seed germination Effects 0.000 description 3
- 241000221017 Euphorbiaceae Species 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 208000032544 Cicatrix Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 229930184510 Mallotus Natural products 0.000 description 1
- 241001060384 Mallotus <angiosperm> Species 0.000 description 1
- 240000005468 Mallotus paniculatus Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000037237 body shape Effects 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000037387 scars Effects 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
本发明公开了一种促进白楸种子快速发芽的方法，包括步骤:将成熟的白楸种子使用草木灰溶液浸泡；种子打磨；清洗后恒温加热；放入赤霉素溶液中浸泡，浸泡后清洗；放入泥炭基质中播种，每两天浇一次水。本发明通过草木灰溶液浸泡、打磨、赤霉素溶液浸泡等多种工序处理，能有效除去白楸种子外的蜡质层，成功提高了白楸种子的发芽率和降低了发芽所需时间。The invention discloses a method for promoting the rapid germination of Catalpa chinensis seeds, which comprises the steps of: soaking mature Catalpinia chinensis seeds in a plant ash solution; grinding the seeds; heating at a constant temperature after cleaning; soaking in a gibberellin solution, and washing after soaking; Plant in a peat substrate and water every two days. The invention can effectively remove the waxy layer outside the Catalpa chinensis seeds through various processes such as plant ash solution soaking, polishing, gibberellin solution soaking, and successfully improve the germination rate and reduce the time required for germination.
Description
技术领域technical field
本发明属于植物种质资源保存技术领域，具体涉及一种促进白楸种子快速发芽的方法。The invention belongs to the technical field of plant germplasm resource preservation, and in particular relates to a method for promoting rapid germination of Catalpa chinensis seeds.
背景技术Background technique
白楸(Mallotus paniculatus(Lam.)Muell.Arg.),是大戟科野桐属(Euphorbiaceae，Mallotus Lour.)灌木或乔木树种；树种高3-15米；种子近球形，深褐色，常具皱纹；花期7-10月，果期11-12月；产于云南、贵州、广西、广东、海南、福建和台湾；生于海拔50-1300米林缘或灌丛中；木材质地轻软,种子油可作工业用油。Catalpa (Mallotus paniculatus (Lam.) Muell.Arg.), is a shrub or tree species of Euphorbiaceae (Euphorbiaceae, Mallotus Lour.); tree species height 3-15 meters; seeds nearly spherical, dark brown, often with Wrinkle; flowering from July to October, fruit from November to December; produced in Yunnan, Guizhou, Guangxi, Guangdong, Hainan, Fujian and Taiwan; born in forest margins or shrubs at an altitude of 50-1300 meters; the wood texture is light and soft, Seed oil can be used as industrial oil.
由于白楸种子外富有蜡质层，种子萌发受到抑制。采用传统播种方法，白楸种子需要近一个月的时间才会萌发，且发芽率只有25％左右。由于每一种植物的体态、特征并不相同，每种植物之间的萌发方法很难借鉴，目前缺乏对于针对于白楸种子萌发的研究和相关报道。Seed germination was inhibited due to the rich waxy layer outside the Catalpa seeds. Using the traditional sowing method, it takes nearly a month for the seeds of Catalpa to germinate, and the germination rate is only about 25%. Due to the different body shape and characteristics of each plant, the germination method between each plant is difficult to learn from, and there is currently a lack of research and related reports on the germination of Catalpa chinensis seeds.
专利CN107182344A公开了一种促进低温保存后白楸种子萌发的方法，但此方法是针对于经过低温储藏(-18℃至-20℃)的白楸种子再萌发的研究；且此专利中记载的种子萌发率为6.7％。并没有涉及提高新鲜种子萌发率的相关研究。Patent CN107182344A discloses a method for promoting the germination of Catalpa chinensis seeds after cryopreservation, but this method is aimed at the research on the re-germination of Catalpa chinensis seeds after low temperature storage (-18 ℃ to -20 ℃); The seed germination rate was 6.7%. There are no related studies on improving the germination rate of fresh seeds.
专利CN107155444A公开了一种促进白背叶种子萌发的方法，此专利针对的是新鲜种子萌发的研究，其种子萌发率接近66.7％。仍有进一步提升的空间。The patent CN107155444A discloses a method for promoting the germination of white-backed leaves. This patent is aimed at the research on the germination of fresh seeds, and the seed germination rate is close to 66.7%. There is still room for further improvement.
发明内容SUMMARY OF THE INVENTION
本发明目的是提供一种促进白楸种子快速发芽的方法，针对于新鲜白楸种子提高其萌发率。The purpose of the present invention is to provide a method for promoting the rapid germination of Catalpa chinensis seeds, and to improve the germination rate of fresh Catalpa chinensis seeds.
本发明为解决其技术问题所采用的技术方案是：The technical scheme adopted by the present invention for solving its technical problem is:
一种促进白楸种子快速发芽的方法，其包括以下步骤：A method for promoting the rapid germination of catalpa seeds, comprising the following steps:
(1)将成熟的白楸种子使用4-6％浓度的草木灰溶液浸泡2-4小时；(1) using the plant ash solution of 4-6% concentration to soak the mature catalpa seeds for 2-4 hours;
(2)经步骤(1)处理后的种子打磨除去蜡质层；(2) polished the seeds after step (1) to remove the waxy layer;
(3)经步骤(2)处理后的种子清洗后放入35℃-60℃中恒温加热1-2小时；(3) the seeds treated in step (2) are cleaned and placed in 35°C-60°C for constant temperature heating for 1-2 hours;
(4)经步骤(3)处理后的种子放入150-400mg/l的赤霉素溶液中浸泡12小时，浸泡后清洗；(4) the seeds treated by step (3) are put into the gibberellin solution of 150-400mg/l and soaked for 12 hours, and cleaned after soaking;
(5)经步骤(4)处理后的种子放入泥炭基质中播种，每两天浇一次水。(5) The seeds treated in step (4) are placed in a peat substrate for sowing, and watered every two days.
进一步地，所述步骤(1)所述的成熟的白楸种子为当年采收并经过去壳的种子,优选为饱满、无病虫害、表面无明显伤痕的种子.Further, the described step (1) mature Catalpa chinensis seeds are the seeds that were harvested in the same year and passed through hulling, preferably the seeds that are plump, free from diseases and insect pests, and without obvious scars on the surface.
进一步地，所述步骤(2)中为种子和沙混合后，揉搓打磨2-5分钟。更进一步地，所述步骤(2)中使用的沙为含水量≥50％的湿沙。Further, in the step (2), after the seeds and sand are mixed, rub and polish for 2-5 minutes. Further, the sand used in the step (2) is wet sand with a water content ≥ 50%.
进一步地，所述步骤(3)中使用水浴锅恒温加热。Further, in the step (3), a water bath is used for constant temperature heating.
进一步地，所述步骤(5)中使用的泥炭基质中泥炭和珍珠岩的质量比为3：1-2：1。Further, the mass ratio of peat and perlite in the peat matrix used in the step (5) is 3:1-2:1.
本发明通过草木灰溶液浸泡、打磨、赤霉素溶液浸泡等多种工序处理，能有效除去白楸种子外的蜡质层，操作方便，成本低。成功提高了白楸种子的发芽率和降低了发芽所需时间。The invention can effectively remove the waxy layer outside the seeds of Catalpa chinensis through various processes such as soaking in plant ash solution, polishing, and soaking in gibberellin solution, and has the advantages of convenient operation and low cost. Successfully increased the germination rate of Catalpa chinensis seeds and reduced the time required for germination.
具体实施方式Detailed ways
下面具体实施例对本发明作进一步说明，以使本领域技术人员可以更好的理解本发明并能予以实施，但所举实施例不作为对本发明的限定。The following specific examples further illustrate the present invention, so that those skilled in the art can better understand the present invention and implement it, but the examples are not intended to limit the present invention.
实施例1：Example 1:
1.材料和方法1. Materials and methods
1.1研究材料：2018年12月15号，深圳市大鹏半岛杨梅坑路边采集新鲜成熟的白楸种子，采集后带回学校进行室内储藏。1.1 Research materials: On December 15, 2018, fresh and mature seeds were collected from Yangmeikeng roadside in Dapeng Peninsula, Shenzhen, and brought back to the school for indoor storage after collection.
1.2研究方法：1.2 Research methods:
1.2.1选种：成熟果实用手揉搓后，将果实去掉外壳杂质，留下饱满、无病虫害、成熟的种子；1.2.1 Seed selection: After the mature fruit is rubbed by hand, the outer shell impurities are removed from the fruit, leaving plump, disease-free and mature seeds;
1.2.2草木灰溶液浸泡：由于种子附有蜡质层，将筛选的成熟种子，用5％的草木灰溶液浸泡2小时以除去蜡质层；1.2.2 Soak in plant ash solution: Since the seeds are attached with waxy layer, the mature seeds that are screened are soaked in 5% plant ash solution for 2 hours to remove the waxy layer;
1.2.3湿沙打磨：将经过5％的草木灰溶液浸泡处理后的种子与含水量≥50％的湿沙混合，用手揉搓打磨2分钟；1.2.3 Wet sand grinding: Mix the seeds soaked in 5% plant ash solution with wet sand with a water content ≥ 50%, and rub and polish by hand for 2 minutes;
1.2.4温水处理实验设计和方法：将种子分为两组处理，分别为对照组和温水处理组，温水处理组的温度为40℃、60℃两个梯度，每个处理三个重复，每个重复30粒。具体方法为：将湿沙打磨处理后的种子，清洗后去掉沙，分别放入40℃、60℃水浴锅中恒温加热2小时，加热后捞出；1.2.4 Experimental design and method of warm water treatment: The seeds were divided into two groups, the control group and the warm water treatment group. The temperature of the warm water treatment group was two gradients of 40 °C and 60 °C, and each treatment was repeated three times. Repeat 30 capsules. The specific method is as follows: the seeds treated with wet sand are polished, and the sand is removed after cleaning.
1.2.5赤霉素处理实验设计和方法：将种子分为两组处理，分别为对照组和赤霉素处理组，赤霉素处理组的赤霉素浓度为200㎎.l-1、300㎎.l-1，每个处理三个重复，每个重复30粒。具体方法为：将温水处理后的种子，分别放入200㎎.l-1、300㎎.l-1的赤霉素溶液中浸泡12小时，捞出清水洗净；1.2.5 The experimental design and method of gibberellin treatment: the seeds were divided into two groups, the control group and the gibberellin treatment group. The gibberellin concentration of the gibberellin treatment group was 200㎎.l -1 , 300 ㎎.l -1 , three replicates for each treatment, 30 capsules for each replicate. The concrete method is as follows: the seeds after the warm water treatment are respectively put into the gibberellin solution of 200㎎.l -1 and 300㎎.l -1 and soaked for 12 hours, then take out clean water and wash;
1.2.6泥炭基质播种：将赤霉素处理后种子，分别播种在泥炭基质中，泥炭：珍珠岩＝3:1，两天浇一次水；试验开始四周后连续两周没有萌发即为结束试验。1.2.6 Sowing in peat matrix: The seeds treated with gibberellin were sown in peat matrix, peat: perlite = 3:1, watered once every two days; the test was terminated if there was no germination for two consecutive weeks after the start of the test for four weeks. .
1.2.5结果分析：对照组35d发芽率为23.3％，温水处理组中40℃发芽率最高为33％，赤霉素处理组中300㎎.l-1发芽率为40％，经过40℃温水处理和300㎎.l-1赤霉素同时处理后的发芽率为73.3％。1.2.5 Results analysis: The germination rate of the control group at 35 days was 23.3%, the highest germination rate at 40°C in the warm water treatment group was 33%, and the 300㎎.l -1 germination rate in the gibberellin treatment group was 40%. After 40°C warm water The germination rate after treatment with 300㎎.l -1 gibberellin was 73.3%.
实施例2：Example 2:
1.材料和方法：1. Materials and methods:
1.1研究材料：2018年12月28号，深圳市大鹏半岛杨梅坑路边采集新鲜成熟的白楸种子，采集后带回学校进行室内储藏。1.1 Research materials: On December 28, 2018, fresh and mature seeds were collected from Yangmeikeng Roadside in Dapeng Peninsula, Shenzhen, and brought back to the school for indoor storage after collection.
1.2研究方法：1.2 Research methods:
1.2.1选种：成熟果实用手揉搓后，将果实去掉外壳杂质，留下饱满、无病虫害、成熟的种子；1.2.1 Seed selection: After the mature fruit is rubbed by hand, the outer shell impurities are removed from the fruit, leaving plump, disease-free and mature seeds;
1.2.2草木灰溶液浸泡：由于种子附有蜡质层，将筛选的成熟种子，用5％的草木灰溶液浸泡2小时；1.2.2 Soak in plant ash solution: Because the seeds are attached with a waxy layer, soak the screened mature seeds with 5% plant ash solution for 2 hours;
1.2.3湿沙打磨：将经过5％的草木灰溶液浸泡处理后的种子与湿沙混合，用手揉搓打磨2分钟；1.2.3 Wet sand sanding: Mix the seeds soaked in 5% plant ash solution with wet sand, rub and sand by hand for 2 minutes;
1.2.4温水处理实验设计和方法：将种子分为两组处理，分别为对照组和温水处理组，温水处理组的温度为40℃、60℃两个梯度，每个处理三个重复，每个重复30粒。具体方法为：将湿沙打磨处理后的种子，清洗后去掉沙，分别放入40℃、60℃水浴锅中恒温加热2小时，加热后捞出；1.2.4 Experimental design and method of warm water treatment: The seeds were divided into two groups, the control group and the warm water treatment group. The temperature of the warm water treatment group was two gradients of 40 °C and 60 °C, and each treatment was repeated three times. Repeat 30 capsules. The specific method is as follows: the seeds treated with wet sand are polished, and the sand is removed after cleaning.
1.2.5赤霉素处理实验设计和方法：将种子分为两组处理，分别为对照组和赤霉素处理组，赤霉素处理组的赤霉素浓度为2001.2.5 Experimental design and method of gibberellin treatment: The seeds were divided into two groups, the control group and the gibberellin treatment group. The gibberellin concentration of the gibberellin treatment group was 200
㎎.l-1、300㎎.l-1，每个处理三个重复，每个重复30粒。具体方法为：将温水处理后的种子，分别放入200㎎.l-1、300㎎.l-1的赤霉素溶液中浸泡12小时，捞出清水洗净；㎎.l -1 , 300㎎.l -1 , three replicates for each treatment, 30 capsules for each replicate. The concrete method is as follows: the seeds after the warm water treatment are respectively put into the gibberellin solution of 200㎎.l -1 and 300㎎.l -1 and soaked for 12 hours, then take out clean water and wash;
1.2.6泥炭基质播种：将赤霉素处理后种子，分别播种在泥炭基质中，泥炭：珍珠岩＝3:1，两天浇一次水；试验开始四周后连续两周没有萌发即为结束试验。1.2.6 Sowing in peat matrix: The seeds treated with gibberellin were sown in peat matrix, peat: perlite = 3:1, watered once every two days; the test was terminated if there was no germination for two consecutive weeks after the start of the test for four weeks. .
1.2.5结果分析：对照组35d发芽率为26.5％，温水处理组中40℃发芽率最高为30％，赤霉素处理组中300㎎.l-1发芽率为35.5％，经过40℃温水处理和300㎎.l-1赤霉素一起处理后的发芽率为72％。1.2.5 Results analysis: The germination rate of the control group at 35 days was 26.5%, the highest germination rate at 40°C in the warm water treatment group was 30%, and the 300㎎.l -1 germination rate in the gibberellin treatment group was 35.5%. After 40°C warm water The germination rate after treatment with 300㎎.l -1 gibberellin was 72%.
实施例3：Example 3:
1.材料和方法：1. Materials and methods:
1.1研究材料：与实施例2相同。1.1 Research materials: the same as in Example 2.
1.2研究方法：1.2 Research methods:
1.2.1选种：成熟果实用手揉搓后，将果实去掉外壳杂质，留下饱满、无病虫害、成熟的种子；1.2.1 Seed selection: After the mature fruit is rubbed by hand, the outer shell impurities are removed from the fruit, leaving plump, disease-free and mature seeds;
1.2.2草木灰溶液浸泡：由于种子附有蜡质层，将筛选的成熟种子，用4％的草木灰溶液浸泡2小时；1.2.2 Soak in plant ash solution: Because the seeds are attached with a waxy layer, soak the screened mature seeds with 4% plant ash solution for 2 hours;
1.2.3湿沙打磨：将经过4％的草木灰溶液浸泡处理后的种子与湿沙混合，用手揉搓打磨2分钟；1.2.3 Wet sand sanding: Mix the seeds soaked in 4% plant ash solution with wet sand, rub and sand by hand for 2 minutes;
1.2.4温水处理实验设计和方法：将种子分为两组处理，分别为对照组和温水处理组，温水处理组的温度为35℃、55℃两个梯度，每个处理三个重复，每个重复30粒。具体方法为：将湿沙打磨处理后的种子，清洗后去掉沙，分别放入35℃、55℃水浴锅中恒温加热1.5小时，加热后捞出；1.2.4 Experimental design and method of warm water treatment: The seeds were divided into two groups, the control group and the warm water treatment group. The temperature of the warm water treatment group was two gradients of 35 °C and 55 °C, and each treatment was repeated three times. Repeat 30 capsules. The specific method is as follows: the seeds treated with wet sand are polished, and the sand is removed after cleaning.
1.2.5赤霉素处理实验设计和方法：将种子分为两组处理，分别为对照组和赤霉素处理组，赤霉素处理组的赤霉素浓度为150㎎.l-1、400㎎.l-1，每个处理三个重复，每个重复30粒。具体方法为：将温水处理后的种子，分别放入150㎎.l-1、400㎎.l-1的赤霉素溶液中浸泡16小时，捞出清水洗净；1.2.5 The experimental design and method of gibberellin treatment: the seeds were divided into two groups for treatment, namely the control group and the gibberellin treatment group. The gibberellin concentration of the gibberellin treatment group was 150㎎.l -1 , 400 ㎎.l -1 , three replicates for each treatment, 30 capsules for each replicate. The concrete method is as follows: the seeds after the warm water treatment are respectively put into the gibberellin solution of 150㎎.l -1 and 400㎎.l -1 and soaked for 16 hours, then take out clean water and wash;
1.2.6泥炭基质播种：将赤霉素处理后种子，分别播种在泥炭基质中，泥炭：珍珠岩＝3:1.2，两天浇一次水；试验开始四周后连续两周没有萌发即为结束试验。1.2.6 Sowing in peat matrix: the seeds treated with gibberellin were sown in peat matrix, peat: perlite = 3:1.2, watered once every two days; the test was ended if there was no germination for two consecutive weeks after the start of the test .
1.2.5结果分析：对照组35d发芽率为25.0％，温水处理组中35℃发芽率最高为30％，赤霉素处理组中400㎎.l-1发芽率为34.0％，经过35℃温水处理和400㎎.l-1赤霉素一起处理后的发芽率为67％。1.2.5 Results analysis: The germination rate of the control group at 35 days was 25.0%, the highest germination rate at 35°C in the warm water treatment group was 30%, and the germination rate at 400㎎.l -1 in the gibberellin treatment group was 34.0%. After 35°C warm water The germination rate after treatment with 400㎎.l -1 gibberellin was 67%.
实施例4：Example 4:
1.材料和方法：1. Materials and methods:
1.1研究材料：与实施例2相同；1.1 Research materials: same as Example 2;
1.2研究方法：1.2 Research methods:
1.2.1选种：成熟果实用手揉搓后，将果实去掉外壳杂质，留下饱满、无病虫害、成熟的种子；1.2.1 Seed selection: After the mature fruit is rubbed by hand, the outer shell impurities are removed from the fruit, leaving plump, disease-free and mature seeds;
1.2.2草木灰溶液浸泡：由于种子附有蜡质层，将筛选的成熟种子，用6％的草木灰溶液浸泡4小时；1.2.2 Soak in plant ash solution: Because the seeds are attached with a waxy layer, soak the screened mature seeds with 6% plant ash solution for 4 hours;
1.2.3湿沙打磨：将经过6％的草木灰溶液浸泡处理后的种子与湿沙混合，用手揉搓打磨5分钟；1.2.3 Wet sand sanding: Mix the seeds soaked in 6% plant ash solution with wet sand, rub and sand by hand for 5 minutes;
1.2.4温水处理实验设计和方法：将种子分为两组处理，分别为对照组和温水处理组，温水处理组的温度为45℃、60℃两个梯度，每个处理三个重复，每个重复30粒。具体方法为：将湿沙打磨处理后的种子，清洗后去掉沙，分别放入45℃、60℃水浴锅中恒温加热2小时，加热后捞出；1.2.4 Experimental design and method of warm water treatment: The seeds were divided into two groups, the control group and the warm water treatment group. The temperature of the warm water treatment group was two gradients of 45 °C and 60 °C, and each treatment was repeated three times. Repeat 30 capsules. The specific method is as follows: the seeds treated with wet sand are polished, washed and removed from the sand, respectively placed in a 45°C and 60°C water bath for constant temperature heating for 2 hours, and then removed after heating;
1.2.5赤霉素处理实验设计和方法：将种子分为两组处理，分别为对照组和赤霉素处理组，赤霉素处理组的赤霉素浓度为250㎎.l-1、350㎎.l-1，每个处理三个重复，每个重复30粒。具体方法为：将温水处理后的种子，分别放入250㎎.l-1、350㎎.l-1的赤霉素溶液中浸泡18小时，捞出清水洗净；1.2.5 The experimental design and method of gibberellin treatment: the seeds were divided into two groups of treatment, namely the control group and the gibberellin treatment group, the gibberellin concentration of the gibberellin treatment group was 250㎎.l -1 , 350 ㎎.l -1 , three replicates for each treatment, 30 capsules for each replicate. The concrete method is as follows: the seeds after the warm water treatment are respectively put into the gibberellin solution of 250㎎.l -1 and 350㎎.l -1 and soaked for 18 hours, then take out the clear water and wash;
1.2.6泥炭基质播种：将赤霉素处理后种子，分别播种在泥炭基质中，泥炭：珍珠岩＝2:1，两天浇一次水；试验开始四周后连续两周没有萌发即为结束试验。1.2.6 Sowing in peat matrix: the seeds treated with gibberellin were sown in peat matrix respectively, peat: perlite = 2:1, watered once every two days; the test was terminated if there was no germination for two consecutive weeks after the start of the test for four weeks. .
1.2.5结果分析：对照组35d发芽率为23.5％，温水处理组中45℃发芽率最高为33％，赤霉素处理组中350㎎.l-1发芽率为41.0％，经过45℃温水处理和350㎎.l-1赤霉素一起处理后的发芽率为74％。1.2.5 Results analysis: The germination rate of the control group at 35 days was 23.5%, the highest germination rate at 45°C in the warm water treatment group was 33%, and the 350㎎.l -1 germination rate in the gibberellin treatment group was 41.0%. After 45°C warm water The germination rate after treatment with 350㎎.l -1 gibberellin was 74%.
本发明的上述实施例并不是对本发明保护范围的限定，本发明的实施方式不限于此，凡此种种根据本发明的上述内容，按照本领域的普通技术知识和惯用手段，在不脱离本发明上述基本技术思想前提下，对本发明上述结构做出的其它多种形式的修改、替换或变更，均应落在本发明的保护范围。The above-mentioned embodiments of the present invention are not intended to limit the protection scope of the present invention, and the embodiments of the present invention are not limited thereto. All of the above-mentioned contents of the present invention, in accordance with common technical knowledge and conventional means in the field, do not depart from the present invention. Under the premise of the above-mentioned basic technical idea, other various modifications, replacements or changes made to the above-mentioned structure of the present invention shall fall within the protection scope of the present invention.
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