CN110763531A - 用于从颗粒悬浮液中分离单颗粒的设备和方法 - Google Patents

用于从颗粒悬浮液中分离单颗粒的设备和方法 Download PDF

Info

Publication number
CN110763531A
CN110763531A CN201910665596.XA CN201910665596A CN110763531A CN 110763531 A CN110763531 A CN 110763531A CN 201910665596 A CN201910665596 A CN 201910665596A CN 110763531 A CN110763531 A CN 110763531A
Authority
CN
China
Prior art keywords
droplet
droplet dispenser
suspension sample
sample
suspension
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910665596.XA
Other languages
English (en)
Chinese (zh)
Inventor
德里克·博格达诺夫
埃里克·周
迈克尔·威尔逊
阿里埃勒·劳伦·格林菲尔德
乔舒亚·坎特隆-布鲁斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sinan AG
University of California
Original Assignee
Sinan AG
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sinan AG, University of California filed Critical Sinan AG
Publication of CN110763531A publication Critical patent/CN110763531A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0265—Drop counters; Drop formers using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0622—Valves, specific forms thereof distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G01N2001/385—Diluting, dispersing or mixing samples diluting by adsorbing a fraction of the sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G01N2035/1058—General features of the devices using the transfer device for another function for mixing

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201910665596.XA 2018-07-23 2019-07-23 用于从颗粒悬浮液中分离单颗粒的设备和方法 Pending CN110763531A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18185007.4 2018-07-23
EP18185007.4A EP3599021B1 (de) 2018-07-23 2018-07-23 Vorrichtung und verfahren zum isolieren von einzelnen partikeln aus einer partikelsuspension

Publications (1)

Publication Number Publication Date
CN110763531A true CN110763531A (zh) 2020-02-07

Family

ID=63165172

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910665596.XA Pending CN110763531A (zh) 2018-07-23 2019-07-23 用于从颗粒悬浮液中分离单颗粒的设备和方法

Country Status (4)

Country Link
US (1) US12000852B2 (de)
EP (1) EP3599021B1 (de)
JP (1) JP7378994B2 (de)
CN (1) CN110763531A (de)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111624085A (zh) * 2020-07-29 2020-09-04 天津中新科炬生物制药股份有限公司 一种用于新型冠状病毒抗体检测的样本稀释装置及试剂卡
CN116355725A (zh) * 2023-03-07 2023-06-30 广州市艾贝泰生物科技有限公司 分配器、分配装置及分配方法

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1315913A (zh) * 1998-07-07 2001-10-03 笛卡尔技术公司 用于微量液体移液的管尖设计和随机存取系统
US20020001675A1 (en) * 1996-07-26 2002-01-03 Thomas C. Tisone Method for dispensing reagent onto substrate
US20020064482A1 (en) * 2000-02-02 2002-05-30 Tisone Thomas C. Method and apparatus for developing DNA microarrays
US20050003458A1 (en) * 2003-06-12 2005-01-06 Mathew Moore Method and system for the analysis of high density cells samples
US20130095469A1 (en) * 2010-06-10 2013-04-18 Albert-Ludwigs-Universitaet Freiburg Apparatus and method for dispensing cells or particles confined in a free flying droplet
WO2013155288A1 (en) * 2012-04-12 2013-10-17 Bio-Rad Laboratories, Inc. Particle dispensing apparatus and method
US20140065704A1 (en) * 2011-04-11 2014-03-06 Masataka Shirai Cell collection system
CN104541146A (zh) * 2012-07-13 2015-04-22 罗氏血液诊断股份有限公司 基片上的样品受控分配
EP3222353A1 (de) * 2016-03-23 2017-09-27 Scienion AG Verfahren und vorrichtung für einzelpartikelabscheidung
US20180056294A1 (en) * 2016-08-29 2018-03-01 The Regents Of The University Of California Integrated system for isolation and emulsification of particles and cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2966904B2 (ja) * 1990-08-08 1999-10-25 株式会社日立製作所 細胞の処理方法、および処理装置
US6551557B1 (en) 1998-07-07 2003-04-22 Cartesian Technologies, Inc. Tip design and random access array for microfluidic transfer
US6579724B2 (en) * 2001-09-13 2003-06-17 First Ten Angstroms Dispensing method and apparatus for dispensing very small quantities of fluid
DE102005047131A1 (de) 2005-09-30 2007-04-12 Evotec Technologies Gmbh Verfahren und Vorrichtung zur Manipulation von sedimentierenden Partikeln

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020001675A1 (en) * 1996-07-26 2002-01-03 Thomas C. Tisone Method for dispensing reagent onto substrate
CN1315913A (zh) * 1998-07-07 2001-10-03 笛卡尔技术公司 用于微量液体移液的管尖设计和随机存取系统
US20020064482A1 (en) * 2000-02-02 2002-05-30 Tisone Thomas C. Method and apparatus for developing DNA microarrays
US20050003458A1 (en) * 2003-06-12 2005-01-06 Mathew Moore Method and system for the analysis of high density cells samples
EP3323877A1 (de) * 2003-06-12 2018-05-23 Accupath Diagnostic Laboratories, Inc. Verfahren und system zur analyse von proben von zellen mit hoher dichte
US20130095469A1 (en) * 2010-06-10 2013-04-18 Albert-Ludwigs-Universitaet Freiburg Apparatus and method for dispensing cells or particles confined in a free flying droplet
US20140065704A1 (en) * 2011-04-11 2014-03-06 Masataka Shirai Cell collection system
CN104321634A (zh) * 2012-04-12 2015-01-28 生物辐射实验室股份有限公司 颗粒分配装置和方法
WO2013155288A1 (en) * 2012-04-12 2013-10-17 Bio-Rad Laboratories, Inc. Particle dispensing apparatus and method
CN104541146A (zh) * 2012-07-13 2015-04-22 罗氏血液诊断股份有限公司 基片上的样品受控分配
EP3222353A1 (de) * 2016-03-23 2017-09-27 Scienion AG Verfahren und vorrichtung für einzelpartikelabscheidung
US20170274689A1 (en) * 2016-03-23 2017-09-28 Scienion Ag Method and apparatus for single particle deposition
US20180056294A1 (en) * 2016-08-29 2018-03-01 The Regents Of The University Of California Integrated system for isolation and emulsification of particles and cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
洪龙烨;郭峰;国世上;赵兴中;: "基于液滴微流控芯片的单细胞分离", 湖北大学学报(自然科学版), no. 03, pages 282 - 285 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111624085A (zh) * 2020-07-29 2020-09-04 天津中新科炬生物制药股份有限公司 一种用于新型冠状病毒抗体检测的样本稀释装置及试剂卡
CN116355725A (zh) * 2023-03-07 2023-06-30 广州市艾贝泰生物科技有限公司 分配器、分配装置及分配方法
CN116355725B (zh) * 2023-03-07 2024-04-05 广州市艾贝泰生物科技有限公司 分配器、分配装置及分配方法

Also Published As

Publication number Publication date
US12000852B2 (en) 2024-06-04
EP3599021B1 (de) 2021-04-21
JP7378994B2 (ja) 2023-11-14
EP3599021A1 (de) 2020-01-29
JP2020014461A (ja) 2020-01-30
US20200025783A1 (en) 2020-01-23

Similar Documents

Publication Publication Date Title
AU2022201238B2 (en) Flow cells utilizing surface-attached structures, and related systems and methods
JP5920895B2 (ja) マイクロ流体捕獲渦を使用して不均一溶液から細胞を単離する方法及びデバイス
US8263387B2 (en) Sheath flow devices and methods
EP2731723B1 (de) Verfahren für magnetische trennung
JP6714603B2 (ja) 検体処理チップ、検体処理装置および検体処理方法
CN114302643A (zh) 用于自动化单细胞处理和分析的系统和方法
JP2013541959A5 (de)
JP2017158491A (ja) 検体処理方法、検体処理チップおよび検体処理装置
CN108117984B (zh) 受试体处理方法及受试体处理装置
US20210316303A1 (en) Flow cells utilizing surface-attached structures, and related systems and methods
JP2022544851A (ja) ハイスループット分析及びソーティング、並びにハイスループット分析及びソーティングためのサンプリングインターフェース及びアセンブリ
US12000852B2 (en) Apparatus and method for isolating single particles from a particle suspension
CN107884562B (zh) 粒子的磁标记方法和标记装置
CA3215784A1 (en) Method and device for trapping at least one nucleated cell using at least one electrode for a microfluidic device, and microfluidic device
KR20220160507A (ko) 미소입자 분리장치 및 이를 이용한 미소입자 분리방법
KR20230062808A (ko) 샘플링 디바이스 및 시스템
CN109843438B (zh) 用于隔离微粒的微流控装置、微流控系统和方法
JP6754345B2 (ja) 粒子の磁気標識方法及び標識装置
JP2014083000A (ja) 目的細胞の分離方法およびその目的細胞の分離装置
Zhang et al. On-chip sample preparations for point-of-care cellular analysis of blood

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB02 Change of applicant information
CB02 Change of applicant information

Address after: Berlin, Germany

Applicant after: Sinon Co.,Ltd.

Applicant after: THE REGENTS OF THE University OF CALIFORNIA

Address before: Berlin, Germany

Applicant before: Sinan AG

Applicant before: THE REGENTS OF THE University OF CALIFORNIA

WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20200207