CN110772995A - Barley β -amylase extraction process - Google Patents
Barley β -amylase extraction process Download PDFInfo
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- CN110772995A CN110772995A CN201911019935.3A CN201911019935A CN110772995A CN 110772995 A CN110772995 A CN 110772995A CN 201911019935 A CN201911019935 A CN 201911019935A CN 110772995 A CN110772995 A CN 110772995A
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- 235000007340 Hordeum vulgare Nutrition 0.000 title claims abstract description 33
- 108010019077 beta-Amylase Proteins 0.000 title claims abstract description 28
- 238000000605 extraction Methods 0.000 title claims abstract description 20
- 240000005979 Hordeum vulgare Species 0.000 title abstract 2
- 238000003825 pressing Methods 0.000 claims abstract description 63
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000001125 extrusion Methods 0.000 claims description 69
- 238000001914 filtration Methods 0.000 claims description 36
- 241000209219 Hordeum Species 0.000 claims description 31
- 102000004190 Enzymes Human genes 0.000 claims description 30
- 108090000790 Enzymes Proteins 0.000 claims description 30
- 229940088598 enzyme Drugs 0.000 claims description 30
- 238000000108 ultra-filtration Methods 0.000 claims description 30
- 239000012528 membrane Substances 0.000 claims description 24
- 239000002994 raw material Substances 0.000 claims description 20
- 239000002002 slurry Substances 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 14
- 108090000637 alpha-Amylases Proteins 0.000 claims description 4
- 102000004139 alpha-Amylases Human genes 0.000 claims description 4
- 229940024171 alpha-amylase Drugs 0.000 claims description 4
- 108090000145 Bacillolysin Proteins 0.000 claims description 3
- 101710130006 Beta-glucanase Proteins 0.000 claims description 3
- 102000035092 Neutral proteases Human genes 0.000 claims description 3
- 108091005507 Neutral proteases Proteins 0.000 claims description 3
- 229910000831 Steel Inorganic materials 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 238000002386 leaching Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000010959 steel Substances 0.000 claims description 3
- 230000001502 supplementing effect Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 12
- 229940079919 digestives enzyme preparation Drugs 0.000 abstract description 2
- 239000012065 filter cake Substances 0.000 description 41
- 229920002472 Starch Polymers 0.000 description 11
- 235000019698 starch Nutrition 0.000 description 11
- 239000008107 starch Substances 0.000 description 11
- 238000011085 pressure filtration Methods 0.000 description 8
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 5
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 5
- 239000006188 syrup Substances 0.000 description 5
- 235000020357 syrup Nutrition 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000000717 retained effect Effects 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 244000017020 Ipomoea batatas Species 0.000 description 2
- 235000002678 Ipomoea batatas Nutrition 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 235000013405 beer Nutrition 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000005056 compaction Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 241000194105 Paenibacillus polymyxa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/18—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/08—Flat membrane modules
- B01D63/082—Flat membrane modules comprising a stack of flat membranes
Landscapes
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention belongs to the technical field of biological enzyme preparations, and particularly relates to a barley β -amylase extraction process, wherein a filter pressing device used in the process comprises two crossbeams, a hydraulic cylinder is fixed at one end of each crossbeam, a pressing plate is fixed at the output shaft end of the hydraulic cylinder, the hydraulic cylinder is used for driving the pressing plate to move, a baffle is fixed at the other end of each crossbeam far away from the hydraulic cylinder, more than two filter frames are movably arranged between the two crossbeams, and water pipes are arranged at the top ends of the pressing plate and the baffle.
Description
Technical Field
The invention belongs to the technical field of biological enzyme preparations, and particularly relates to a barley β -amylase extraction process.
Background
β -amylase (β -amylase), also called starch β -1, 4-maltosidase, is one of amylase class, widely exists in higher plants such as barley, wheat, sweet potato, soybean, etc. and in microorganisms such as Bacillus, is the main saccharifying agent for beer brewing and maltose (maltose syrup) production, β -amylase produced by microorganisms such as Bacillus polymyxa, Bacillus megaterium, etc. is used to saccharify acidified or α -amylase liquefied starch raw materials, and can produce high maltose syrup with maltose content of 60% -70%.
When the barley β -amylase is used for obtaining a concentrated enzyme solution, a filter pressing step is needed, but the traditional steps are complicated, time and labor are consumed, moisture is contained in a filter cake in the filter pressing process, the loss of a filter pressing solution is caused, in addition, the filter cake is not easy to separate from a filter pressing device, the filter pressing efficiency of the enzyme solution is low, and the extraction of the barley β -amylase is influenced, so that the barley β -amylase extraction process is provided.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides a barley β -amylase extraction process, wherein a filter pressing device used in the process realizes the graded ultrafiltration concentration of β -amylase raw material slurry through ultrafiltration membranes with different apertures, the concentration efficiency of the raw material slurry is improved, and meanwhile, an extrusion air bag is arranged to extrude filter residues, so that water in the filter residues is fully extruded out, the utilization rate of the raw material slurry is increased, and the filter pressing efficiency of the filter pressing device is improved.
The technical scheme adopted by the invention for solving the technical problems is that the barley β -amylase extraction process comprises the following steps:
s1, mixing barley and water in proportion, grinding the mixture in a refiner, transferring the ground raw material slurry into a test tube, adding α -amylase, β -glucanase and neutral protease into the raw material slurry, and performing enzymolysis and leaching for 1-3 hours;
s2: carrying out fractional ultrafiltration concentration on the raw material slurry in the S1 through a filter pressing device to obtain a concentrated enzyme solution, heating the concentrated enzyme solution to 25-45 ℃, removing thermal condensate in the concentrated enzyme solution, simultaneously adjusting the pH value of the concentrated enzyme solution to 4, and removing impure protein in the concentrated enzyme solution through an isoelectric point method;
s3: carrying out fine filtration treatment on the concentrated enzyme liquid subjected to impurity removal treatment in S2 to obtain high-purity enzyme liquid, and sealing and storing the enzyme liquid;
the method comprises the following steps that preferably, a filter pressing device used in S2 comprises two crossbeams, a hydraulic cylinder is fixed to one end of each crossbeam, a pressing plate is fixed to an output shaft end of each hydraulic cylinder, the hydraulic cylinders are used for driving the pressing plates to move, a baffle is fixed to the other end, far away from the hydraulic cylinders, of each crossbeam, more than two filter frames are movably arranged between the two crossbeams, water pipes are arranged at top ends of the pressing plates and the baffle and are communicated with inner cavities of the filter frames respectively, rectangular frames are fixed to side walls of the filter frames along the length direction of the crossbeams, extrusion air bags are fixed in the rectangular frames, four corners of the extrusion air bags are fixed to inner side walls of the rectangular frames through steel wire ropes, the extrusion air bags are connected with air pumps through connecting pipes, the extrusion air bags are used for extruding, ultrafiltration membranes are fixed to one side of the filter frames far away from the baffle, a controller and a hydraulic station are arranged at the bottom of the crossbeams and used for controlling the filter pressing device to work, the hydraulic station is used for controlling the filter pressing device to control the filter pressing work, the hydraulic cylinder, and the hydraulic station is used for controlling the filter pressing work, and the filter pressing of the air bags, when the filter pressing device, β -amylase β -amylase is used for pressing, so that starch sugar solution, so that starch syrup is not easily extruded, so that starch glucoside 54-1, 4- β -amylase is retained in a starch syrup, a filtration plant air bag, a filtration machine is not easily retained in a filtration machine, a filtration machine is not easily retained in a filtration machine, a filtration machine is used for pressing machine, a filtration machine is used for pressing machine, a filtration machine for pressing a filtration process, a filtration process for pressing machine for pressing a filtration process for pressing a filtration, a filtration process for pressing a filtration, a filtration process for pressing a filtration, a filtration process.
Preferably, a folding airbag is arranged between the adjacent filter frames, two ends of the folding airbag are respectively fixed on the side walls of the adjacent filter frames, the folding airbag is communicated with the extrusion airbag through an air pipe, an electromagnetic valve is arranged in the middle of the air pipe, the folding airbag is used for supplementing gas for the extrusion airbag, during operation, the extrusion airbag needs to be inflated during operation, the extrusion airbag needs larger pressure during inflation, the requirement on an air pump is larger, the folding airbag is arranged, when the filter frames are pressed, the folding airbag is compressed, so that the gas pressure in the folding airbag is increased, when the extrusion airbag extrudes a filter cake, the electromagnetic valve is opened by a controller, so that the gas in the folding airbag enters the extrusion airbag, the extrusion airbag is pressurized, the expansion degree of the extrusion airbag is increased, the extrusion effect of the extrusion airbag is improved, and the compaction degree of the filter cake is further improved, when the filter frames are far away from each other, the folded airbag inhales and stores the gas, so that the folded airbag can continuously work.
Preferably, the four corners of the inner side wall of the filter frame are all fixed with mounting blocks, the side wall of each mounting block is provided with an arc-shaped groove, a connecting rod is movably connected between the diagonal mounting blocks, the middle parts of the connecting rods are connected in a rotating manner through bolts, the two ends of each connecting rod are positioned in the arc-shaped grooves, two elastic ropes are symmetrically fixed between the connecting rods, when the filter press works, a filter cake is positioned between adjacent ultrafiltration membranes and is not easily separated from the filter frame when the filter press works, so that the continuous working efficiency of the filter press device is influenced, the connecting rods are arranged, when the extrusion air bags expand, the extrusion air bags extrude the connecting rods to enable the connecting rods to rotate, the two ends of each connecting rod slide in the arc-shaped grooves, meanwhile, the elastic ropes are tightened by the rotation of the connecting rods, when the extrusion air bags shrink, the acting force of the extrusion air bags on the connecting rods is reduced, the connecting rods generate, the cleaning difficulty of the filter cake is reduced, and the filter pressing efficiency of the filter pressing device is improved.
Preferably, the molecular weight of milipore filter diminishes along with the grow of distance between milipore filter and the clamp plate, the milipore filter is used for concentrated filtration in grades, in operation, the milipore filter molecular weight that is close to clamp plate one end is big, the solute through the milipore filter is many, and the milipore filter molecular weight that keeps away from clamp plate one end is little, the solute through the milipore filter diminishes, make the milipore filter realize the filtration in grades, guaranteed the filtration efficiency of raw materials thick liquid on the one hand, obtain the concentrated enzyme liquid of wanting, on the other hand makes the solute in the raw materials thick liquid reduce gradually through the volume of milipore filter, the filtration burden of milipore filter has been reduced, make the life extension of milipore filter, the.
Preferably, the thickness at extrusion gasbag lateral wall middle part is the twice of extrusion gasbag lateral wall edge, make the even expansion of extrusion gasbag, in operation, the extrusion gasbag expands when aerifing, under the general condition, the middle part position of extrusion gasbag expands earlier, form the globular structure, it is inhomogeneous to the extrusion of filter cake to make the extrusion gasbag, lead to the filter cake to distribute in the edge of filter frame, influence the filtration efficiency of milipore filter, and extrusion gasbag middle part thickness sets up the twice of edge position thickness, make the even inflation of extrusion gasbag, thereby carry out the extrusion of overlay type rather than extruding the filter cake to the edge of filter frame from the middle part of filter frame, the even degree of filter cake has been guaranteed, also make the milipore filter can evenly filter, the filter-pressing efficiency of filter pressing device has been improved.
The invention has the following beneficial effects:
1. according to the barley β -amylase extraction process, the filter pressing device used in the process is provided with the ultrafiltration membranes with the gradually-reduced pore diameters, so that the filter pressing device can be used for carrying out graded concentration on filter pressing slurry, the stress of each ultrafiltration membrane is uniform, the service life of each ultrafiltration membrane is prolonged, the intercepted solutes in the raw material slurry are uniformly distributed in the filter frame, the phenomenon that the solutes are blocked at the ultrafiltration membrane at one end of the pressing plate is avoided, and the filter pressing efficiency is improved.
2. According to the barley β -amylase extraction process, the filter pressing device used in the process is used for fully extruding the water in the filter cake through the extrusion of the extrusion air bag, so that the waste of raw material slurry is reduced, and the extraction efficiency of the enzyme liquid is improved.
3. According to the barley β -amylase extraction process, the filter pressing device used in the process extrudes the filter cake through the connecting rod in the rotating process through the arrangement of the connecting rod, so that the filter cake is quickly crushed and falls off from the filter frame, the separation efficiency of the filter cake and the filter frame is improved, and the filter pressing efficiency of the filter pressing device is directly improved.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a flow chart of the present invention;
FIG. 2 is a three-dimensional view of a press filtration device for use in the present invention;
FIG. 3 is an enlarged view of a portion of FIG. 2 at A;
FIG. 4 is a schematic view of the effect of the filter press apparatus used in the present invention;
FIG. 5 is a three-dimensional view of a filter frame;
FIG. 6 is a right side partial cross-sectional view of the filter frame;
FIG. 7 is a three-dimensional view of a rectangular box;
in the figure: girder 1, pneumatic cylinder 2, clamp plate 3, baffle 4, filter frame 5, water pipe 6, rectangle frame 7, wire rope 71, extrusion gasbag 8, milipore filter 9, folding gasbag 10, installation piece 11, connecting rod 12, arc wall 13, stretch cord 14.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 7, the barley β -amylase extraction process of the invention comprises the following steps:
s1, mixing barley and water in proportion, grinding the mixture in a refiner, transferring the ground raw material slurry into a test tube, adding α -amylase, β -glucanase and neutral protease into the raw material slurry, and performing enzymolysis and leaching for 1-3 hours;
s2: carrying out fractional ultrafiltration concentration on the raw material slurry in the S1 through a filter pressing device to obtain a concentrated enzyme solution, heating the concentrated enzyme solution to 25-45 ℃, removing thermal condensate in the concentrated enzyme solution, simultaneously adjusting the pH value of the concentrated enzyme solution to 4, and removing impure protein in the concentrated enzyme solution through an isoelectric point method;
s3: carrying out fine filtration treatment on the concentrated enzyme liquid subjected to impurity removal treatment in S2 to obtain high-purity enzyme liquid, and sealing and storing the enzyme liquid;
according to a specific embodiment of the invention, a filter pressing device used in S2 comprises two girders 1, a hydraulic cylinder 2 is fixed at one end of each girder 1, a pressing plate 3 is fixed at an output shaft end of each hydraulic cylinder 2, each hydraulic cylinder 2 is used for driving the pressing plate 3 to move, a baffle 4 is fixed at the other end of each girder 1 far away from the hydraulic cylinder 2, two or more filter frames 5 are movably arranged between the two girders 1, water pipes 6 are arranged at the top ends of the pressing plate 3 and the baffle 4, the water pipes 6 respectively penetrate through the pressing plate 3 and the baffle 4 and are communicated with an inner cavity of each filter frame 5, a rectangular frame 7 is fixed on the side wall of each filter frame 5 along the length direction of the girder 1, an extrusion air bag 8 is fixed in the rectangular frame 7, four corners of the extrusion air bag 8 are fixed on the inner side wall of the rectangular frame 7 through steel wire ropes 71, the extrusion air pump is connected with the extrusion air bag 8 through connecting pipes, the extrusion air bag 8 is used for extruding filter residue, one side of the filter pressing air bag 5 is fixed with an air bag 9, the filter cake 5, the cross filter cake 5 is fixed on one side far away from the baffle 4, the crossbeam 9 is used for filtering the filter cake, the girder 9 is used for filtering the filter cake, the girder 9 is used for filtering device, the filter cake 5, the filter cake is arranged at the bottom of the barley cake, a controller and the barley filter cake, the barley filter cake is arranged at a high starch cake, the barley filter cake is more starch cake, the barley filter cake is more starch cake, the barley filter cake is more starch cake, the barley filter cake.
As a specific embodiment of the invention, a folding airbag 10 is arranged between adjacent filter frames 5, two ends of the folding airbag 10 are respectively fixed on the side walls of the adjacent filter frames 5, the folding airbag 10 is communicated with an extrusion airbag 8 through an air pipe, an electromagnetic valve is arranged in the middle of the air pipe, the folding airbag 10 is used for supplementing air for the extrusion airbag 8, when the extrusion airbag 8 works, the extrusion airbag 8 needs to be inflated, when the extrusion airbag 8 is inflated, a large pressure is needed inside the extrusion airbag 8, the requirement on an air pump is large, the folding airbag 10 is arranged, when the filter frames 5 are pressed, the folding airbag 10 is compressed, so that the air pressure inside the folding airbag 10 is increased, when the extrusion airbag 8 presses a filter cake, a controller opens the electromagnetic valve, so that the air in the folding airbag 10 enters the extrusion airbag 8, the extrusion airbag 8 is pressurized, so that the expansion degree of the extrusion airbag 8 is increased, thereby improve the extrusion effect of extrusion gasbag 8, and then improve the degree of compaction of filter cake, when straining frame 5 and keeping away from each other, folding gasbag 10 inhales gas and stores for folding gasbag 10 can last work.
As a specific embodiment of the invention, the four corners of the inner side wall of the filter frame 5 are all fixed with mounting blocks 11, the side wall of the mounting block 11 is provided with an arc-shaped groove 12, a connecting rod 13 is movably connected between the diagonal mounting blocks 11, the middle part of the connecting rod 13 is rotatably connected through a bolt, two ends of the connecting rod 13 are positioned in the arc-shaped groove 12, two elastic ropes 14 are symmetrically fixed between the connecting rods 13, when the filter press is in work, a filter cake is positioned between adjacent ultrafiltration membranes 9 and is not easy to be separated from the filter frame 5 during cleaning, so that the continuous work efficiency of the filter press device is affected, the connecting rod 13 is arranged, when the extrusion air bag 8 expands, the extrusion air bag 8 extrudes the connecting rod 13 to enable the connecting rod 13 to rotate, two ends of the connecting rod 13 slide in the arc-shaped groove 12, meanwhile, the rotation of the connecting rod 13 enables the elastic ropes 14 to be tightened, at the moment, the connecting rod 13 generates reset rotation under the contraction of the elastic rope 14, so that the filter cake is extruded to deform, the filter cake is broken and falls off from the filter frame 5, the cleaning difficulty of the filter cake is reduced, and the filter pressing efficiency of the filter pressing device is improved.
As a specific embodiment of the present invention, the molecular weight of the ultrafiltration membrane 9 decreases as the distance between the ultrafiltration membrane 9 and the pressure plate 3 increases, and the ultrafiltration membrane 9 is used for fractional concentration filtration, and during operation, the ultrafiltration membrane 9 near the end of the pressure plate 3 has a large molecular weight, and the solute passing through the ultrafiltration membrane 9 is large, while the ultrafiltration membrane 9 far from the end of the pressure plate 3 has a small molecular weight, and the solute passing through the ultrafiltration membrane 9 is small, so that the ultrafiltration membrane 9 realizes fractional filtration, thereby ensuring the filtration efficiency of the raw material slurry to obtain the desired concentrated enzyme solution, and on the other hand, gradually reducing the amount of the solute in the raw material slurry passing through the ultrafiltration membrane 9, reducing the filtration burden of the ultrafiltration membrane 9, prolonging the service life of the ultrafiltration membrane 9, and ensuring the pressure filtration balance inside the pressure.
As a specific embodiment of the present invention, the thickness of the middle portion of the sidewall of the extrusion airbag 8 is twice that of the edge of the sidewall of the extrusion airbag 8, so that the extrusion airbag 8 expands uniformly, when the extrusion airbag 8 inflates during operation, the middle portion of the extrusion airbag 8 expands first to form a spherical structure, so that the extrusion of the extrusion airbag 8 on the filter cake is not uniform, the filter cake is distributed at the edge of the filter frame 5, and the filtration efficiency of the ultrafiltration membrane 9 is affected, and the thickness of the middle portion of the extrusion airbag 8 is set to be twice that of the edge portion, so that the extrusion airbag 8 expands uniformly, so that the filter cake is extruded in a covering manner instead of extruding the filter cake from the middle portion of the filter frame 5 to the edge of the filter frame 5, thereby ensuring the uniformity of the filter cake, enabling the ultrafiltration membrane 9 to filter uniformly, and improving the filter pressing efficiency of the filter pressing device.
When the pressure filtration process is carried out, β -amylase β -amyloylase, also called starch β -1, 4-maltosidase, which is one of amylase types and widely exists in high plants such as barley, wheat, sweet potatoes, soybeans and microorganisms such as bacillus, is a main saccharifying agent for brewing beer and manufacturing maltose syrup, when the barley β -amylase obtains concentrated enzyme liquid, a pressure filtration step is needed, the traditional step needs filtration and ultrafiltration after cleaning, is relatively complex and time-consuming, meanwhile, the filter cake in the pressure filtration process is not easy to separate from the pressure filtration device, the pressure filtration efficiency is low, more raw material slurry remains in the filter cake, waste is easy to cause, the extraction efficiency of the barley β -amylase is directly influenced, the filter cake 5 is arranged, when the ultrafiltration operation is carried out, a controller controls a hydraulic station to work, a hydraulic cylinder 2 drives a pressure plate 3 to move, the pressure plate 3 presses the filter cake 3 to the baffle 4, so that a filter chamber is formed by an inner cavity of the filter frame 5, then the water pipe 6 on the pressure filtration plate 3 is introduced, the air bag 6 enters the air bag to drive the pressure filtration frame 3 to move, the air bag to enable the air bag to be cleaned, the air bag to enable the air bag to be in the air bag to be discharged, the air bag to enable the air bag to be discharged from the air bag to enable the air bag to be discharged, the air bag to enable the air bag to be discharged, the air bag to enable the air bag to be discharged, the air bag to enable the air.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A barley β -amylase extraction process is characterized by comprising the following steps:
s1, mixing barley and water in proportion, grinding the mixture in a refiner, transferring the ground raw material slurry into a test tube, adding α -amylase, β -glucanase and neutral protease into the raw material slurry, and performing enzymolysis and leaching for 1-3 hours;
s2: carrying out fractional ultrafiltration concentration on the raw material slurry in the S1 through a filter pressing device to obtain a concentrated enzyme solution, heating the concentrated enzyme solution to 25-45 ℃, removing thermal condensate in the concentrated enzyme solution, simultaneously adjusting the pH value of the concentrated enzyme solution to 4, and removing impure protein in the concentrated enzyme solution;
s3: and (4) carrying out fine filtration treatment on the concentrated enzyme liquid subjected to impurity removal treatment in S2 to obtain high-purity enzyme liquid, and sealing and storing the enzyme liquid.
2. The barley β -amylase extraction process of claim 1, wherein a filter pressing device used in S2 comprises two girders (1), one end of each girder (1) is fixed with a hydraulic cylinder (2), an output shaft end of each hydraulic cylinder (2) is fixed with a pressing plate (3), each hydraulic cylinder (2) is used for driving the pressing plate (3) to move, the other end of each girder (1) far away from the hydraulic cylinder (2) is fixed with a baffle (4), two filter frames (5) more than two are movably arranged between the girders (1), the tops of the pressing plates (3) and the baffles (4) are all provided with a water pipe (6), the water pipes (6) respectively penetrate through the insides of the pressing plates (3) and the baffles (4) and are communicated with inner cavities of the filter frames (5), the filter frames (5) are fixed with rectangular air bags (7) along the side walls of the girders (1) in the length direction, the rectangular air bags (7) are internally fixed with extrusion air bags (8), the four corners of the extrusion air bags (8) are all fixed on the inner side walls of the steel wire ropes (7), the extrusion air bags (8) are connected with an air pump (9), and the extrusion control hydraulic filter press filter frames (9) and hydraulic control hydraulic filter hydraulic control hydraulic pressure filter cylinders (1) and the hydraulic filter frames (9).
3. The barley β -amylase extraction process according to claim 2, wherein a folding air bag (10) is arranged between adjacent filter frames (5), two ends of the folding air bag (10) are respectively fixed on the side walls of the adjacent filter frames (5), the folding air bag (10) is communicated with the extrusion air bag (8) through an air pipe, an electromagnetic valve is arranged in the middle of the air pipe, and the folding air bag (10) is used for supplementing air for the extrusion air bag (8).
4. The barley β -amylase extraction process according to claim 3, wherein mounting blocks (11) are fixed at four corners of the inner side wall of the filter frame (5), arc-shaped grooves (12) are formed in the side wall of each mounting block (11), a connecting rod (13) is movably connected between the diagonal mounting blocks (11), the middle parts of the connecting rods (13) are rotatably connected through bolts, two ends of each connecting rod (13) are located in the arc-shaped grooves (12), and two elastic ropes (14) are symmetrically fixed between the connecting rods (13).
5. The barley β -amylase extraction process of claim 2, wherein the molecular weight of the ultrafiltration membrane (9) becomes smaller as the distance between the ultrafiltration membrane (9) and the pressing plate (3) becomes larger, and the ultrafiltration membrane (9) is used for fractional concentration filtration.
6. The barley β -amylase extraction process of claim 2, wherein the thickness of the middle of the side wall of the extrusion air bag (8) is twice as thick as the edge of the side wall of the extrusion air bag (8), so that the extrusion air bag (8) expands uniformly.
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| CN201911019935.3A CN110772995A (en) | 2019-10-24 | 2019-10-24 | Barley β -amylase extraction process |
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| CN201911019935.3A CN110772995A (en) | 2019-10-24 | 2019-10-24 | Barley β -amylase extraction process |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113199790A (en) * | 2021-04-16 | 2021-08-03 | 梁银清 | Edible oil squeezing process with high oil yield |
| CN114350483A (en) * | 2021-12-21 | 2022-04-15 | 路莹 | Beta amylase production system and production process thereof |
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| CN114350483A (en) * | 2021-12-21 | 2022-04-15 | 路莹 | Beta amylase production system and production process thereof |
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Application publication date: 20200211 |