CN110840396A - Patterned microarray of odor molecules, preparation method and application thereof - Google Patents

Abstract

The invention relates to printing technology, in particular to a patterned microarray of odor molecules and a preparation method and application thereof. The patterned microarray includes a substrate and a core-shell structure formed on the substrate, wherein the core-shell structure uses an odor molecule as a core and a poly(lactic-co-glycolic acid) as a shell. The preparation method includes: printing a suspension containing odor molecules on a substrate according to a predetermined pattern; printing a poly(lactic-co-glycolic acid) solution on the suspension of odor molecules on the substrate in situ; After the solvent in the co-glycolic acid) solution is volatilized, a patterned microarray with the core-shell structure is obtained. The effective odor release properties of the patterned microarrays of odor molecules provided by the present invention make them promising for on-demand odor release, studies of olfactory sensitivity and enhanced olfactory exercise. In addition, the patterned microarray of odor molecules provided by the present invention can have the characteristics of high stability, and will not deform during long-term storage in the atmospheric environment.

Description

Patterned microarray of odor molecules and preparation method and application thereof

technical field

The invention relates to printing technology, in particular to a patterned microarray of odor molecules and a preparation method and application thereof.

Background technique

Smell is a perception experienced by the organs. Humans also have an exceptionally sensitive sense of smell, and it has a great impact on our lives unknowingly. Subtle scents can affect mood, behavior, and decisions without us noticing. The sense of smell declines with age and with the development of olfactory disorders. Among them, olfactory sensitivity is the main indicator for evaluating some neurodegenerative diseases, which can be detected by odor test, but olfactory performance is greatly affected by the type of odor tested.

Deteriorated sense of smell requires treatment, and steps can be taken in everyday life to improve and maintain the sense of smell. One of the measures taken is to continuously "exercise" the olfactory receptors and continuously stimulate the olfactory nerves to delay degeneration. Therefore, a product with multiple types and quantitative release of odor has potential application value in the detection of olfactory sensitivity.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a product that can release odor quantitatively, so as to effectively detect or "exercise" olfactory sensitivity.

In order to achieve the above object, one aspect of the present invention provides a patterned microarray of odor molecules, the patterned microarray includes a substrate and a core-shell structure formed on the substrate, and the core-shell structure takes the odor molecule as a core, With poly(lactic-co-glycolic acid) as the shell.

In a second aspect, the present invention provides a method for preparing a patterned microarray of odor molecules, the method comprising:

(1) printing the suspension containing odor molecules on the substrate according to a predetermined pattern;

(2) The poly(lactic acid-co-glycolic acid) solution is in-situ printed on the suspension of the odor molecules printed on the substrate, and after the solvent in the poly(lactic acid-co-glycolic acid) solution is volatilized to obtain a A patterned microarray of a core-shell structure with odor molecules as the core and poly(lactic-co-glycolic acid) as the shell arranged in a predetermined pattern.

In a third aspect, the present invention provides a patterned microarray prepared by the method as described above.

In a fourth aspect, the present invention provides the use of patterned microarrays as described above in the manufacture of products for odor-on-demand release, olfactory sensitivity detection and olfactory sensitivity exercise.

As a technical means that can precisely distribute micro-nano materials in space and function, inkjet printing technology reflects its unique properties in realizing the integration of materials and functions. This technology directly patterned deposition of various types of specific functional materials in a non-contact way has the advantages of simple method, low cost, flexibility and rapidity, and has become one of the most promising patterning methods. Its promotion and application fields have received extensive attention. In recent years, inkjet printing technology can realize large-area direct writing of microstructures and functional patterns, which is widely used in the patterning of biological functional materials. The inventor of the present invention starts from the existing inkjet printing technology for preparing microstructures, expands the application of inkjet printing, and proposes a simple, efficient, green and environmentally friendly odor patterning technology that can be prepared in a large area. Odor core-shell structures (with odor molecules as the core and poly(lactic-co-glycolic acid) (PLGA) as the shell) microarrays for programmable release of multiple fragrances. After scratching the protective PLGA shell, quantitative concentrations of odorant molecules can be released from the microarray-based chip (the concentration of odor released can be controlled by scratching different numbers of shell layers). By setting the core layer of odor molecules as one or more odors, the release of multiple mixed odors can be achieved. The effective odor release properties of the patterned microarrays of odor molecules provided by the present invention make them promising for on-demand odor release, studies of olfactory sensitivity and enhanced olfactory exercise.

In addition, the patterned microarray of odor molecules provided by the present invention can have the characteristics of high stability, and will not deform during long-term storage in the atmospheric environment.

Description of drawings

The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification, and together with the following specific embodiments, are used to explain the present invention, but do not constitute a limitation to the present invention.

FIG. 1 is a schematic diagram of a patterned microarray of odor molecules prepared by inkjet printing of the present invention.

2 is a detailed view of a patterned microarray of odor molecules prepared by inkjet printing in Example 1 of the present invention.

3 is a patterned microarray diagram of three kinds of odor molecules of longan, milk and vanilla prepared by inkjet printing in Examples 1-3 of the present invention.

4 is a patterned microarray diagram of multi-patterned odor molecules prepared by inkjet printing in Examples 4 and 5 of the present invention.

5 is a patterned microarray diagram of odor molecules prepared by inkjet printing on the surfaces of glass sheets, silicon sheets (hard materials) and PET films (flexible materials) in Example 6 of the present invention.

6 is a patterned microarray diagram of odor molecules of ammonia water odor prepared by inkjet printing in Example 7 of the present invention, and the quantitative release of odor molecules is detected by a precision test paper of pH 8.0-10.0.

Figure 7 shows the stability test results of the products prepared in each example in KMnO ₄ solution.

Detailed ways

The endpoints of ranges and any values disclosed herein are not limited to the precise ranges or values, which are to be understood to encompass values proximate to those ranges or values. For ranges of values, the endpoints of each range, the endpoints of each range and the individual point values, and the individual point values can be combined with each other to yield one or more new ranges of values that Ranges should be considered as specifically disclosed herein.

In a first aspect, the present invention provides a patterned microarray of odor molecules, the patterned microarray comprising a substrate and a core-shell structure formed on the substrate, wherein the core-shell structure takes the odor molecule as the core, and the poly(( lactic acid-co-glycolic acid) as the shell.

According to the present invention, in the core of the core-shell structure, in addition to the odorant molecule, a carrier for the odorant molecule, for example, a solvent for dissolving the odorant molecule, and a carrier for the odorant molecule, and An adjuvant whose molecules are uniformly dispersed in the solvent. According to a preferred embodiment of the present invention, the odorant molecules are present in the form of a suspension of odorant molecules, and the suspension contains odorant molecules, an emulsifier, an alcohol and a solvent. In the suspension, the amount of each component can be selected within a wide range, as long as the odor molecules can be stably and uniformly present in the suspension. In the molecular suspension, the content of the odor molecules is 0.01-50% by weight, the content of the alcohol is 2-3% by weight, and the content of the emulsifier is 0.25-5% by weight, preferably 2-4.5% by weight %.

Preferably, the emulsifier is polyvinyl alcohol.

Preferably, the alcohol is ethylene glycol.

Preferably, the solvent is water.

According to a preferred embodiment of the present invention, the suspension contains odor molecules, polyvinyl alcohol, ethylene glycol and water, wherein the content of the odor molecules is 0.01-50% by weight, the ethylene glycol The content of the polyvinyl alcohol is 2-3% by weight, and the content of the polyvinyl alcohol is 0.25-5% by weight. In this preferred case, the prepared core-shell structure is more stable for storage in an atmospheric environment, and the release of odor molecules is also more stable and easier to control.

According to the present invention, the types of odor molecules are not limited, and can be any substance with odor, for example, but not limited to alcohol, aldehyde, ester, protein, etc., more specifically, can be various flavors , such as but not limited to one or more of milk flavor, longan flavor, vanilla flavor, cola flavor, apple flavor, cumin flavor, lemon flavor, walnut flavor, grape flavor, almond flavor, rose fragrance and ammonia water. Those skilled in the art can choose according to actual needs. In addition, the core-shell structure of the present invention may contain a single odorant molecule, or may contain different odorant molecules, and the different odorant molecules may be a mixture of different odorant molecules in one core-shell structure, or a combination of multiple odorant molecules. A single odor molecule is arranged in different core-shell structures, and different odors can be released according to requirements.

According to the present invention, the substrate can be various substrates, can be rigid substrates, or can be flexible substrates, and the rigid substrates can be but not limited to silicon wafers, glass wafers, quartz wafers, iron wafers, copper wafers, and aluminum wafers. and one of alumina sheets; the flexible substrate can be but not limited to one of PDMS film, PET film, PS film, PU film and PI film.

According to the present invention, the shape of the core-shell structure can be specifically varied according to the surface tension of the suspension and the type of the substrate, and is generally hemispherical. Preferably, the particle size of the core-shell structure is 0.5-1.5 mm.

According to the present invention, preferably, in the core-shell structure, the particle size of the core layer is 0.8-1.0 mm, and the thickness of the shell layer is 0.10-0.15 mm.

According to the present invention, the pattern in the patterned microarray can be any pattern, and can be designed according to specific conditions and actual preferences. According to a specific embodiment of the present invention, the pattern is a lattice pattern of a core-shell structure. Preferably, the lattice pattern is a periodic single-point array. More preferably, the distance between the center lines of two adjacent core-shell structures is 0.5-1.5 cm.

According to the present invention, the preparation method of the patterned microarray is not particularly limited, for example, the suspension containing odor molecules can be spread uniformly on the substrate using fine needles, and then the poly(lactic acid-co- Glycolic acid) solution wraps the spreading suspension liquid, and the core-shell structure is obtained after the solution containing poly(lactic-co-glycolic acid) is dried.

Wherein, the solvent in the solution containing poly(lactic acid-co-glycolic acid) can be any solvent capable of dissolving poly(lactic acid-co-glycolic acid), but since the subsequent drying procedure is involved, therefore, the The solvent is preferably an easily volatile solvent. According to a preferred embodiment of the present invention, the solvent is dichloromethane.

According to the present invention, in the solution containing poly(lactic acid-co-glycolic acid), the concentration of poly(lactic acid-co-glycolic acid) is not particularly limited, considering the stability of the formed core-shell structure Preferably, the concentration of the poly(lactic-co-glycolic acid) is 2-3% by weight.

According to a preferred embodiment of the present invention, the patterned microarray of the odorant molecules is prepared by printing technology. Thus, in a second aspect, as shown in FIG. 1 , the present invention provides a method for preparing a patterned microarray of odor molecules, the method comprising:

(1) printing the suspension containing odor molecules on the substrate according to a predetermined pattern;

(2) The poly(lactic acid-co-glycolic acid) solution is in-situ printed on the suspension of the odor molecules printed on the substrate, and after the solvent in the poly(lactic acid-co-glycolic acid) solution is volatilized to obtain a A patterned microarray of a core-shell structure with odor molecules as the core and poly(lactic-co-glycolic acid) as the shell arranged in a predetermined pattern.

According to the present invention, in addition to the odor molecules, the suspension may also contain a carrier for the odor molecules, such as a solvent for dissolving the odor molecules, and a solvent that helps the odor molecules to disperse uniformly adjuvants in the solvent. According to a preferred embodiment of the present invention, the odorant molecules are present in the form of a suspension of odorant molecules, and the suspension contains odorant molecules, an emulsifier, an alcohol and a solvent. In the suspension, the amount of each component can be selected within a wide range, as long as the odor molecules can be stably and uniformly present in the suspension. In the molecular suspension, the content of the odor molecules is 0.01-50% by weight, the content of the alcohol is 2-3% by weight, and the content of the emulsifier is 0.25-5% by weight, preferably 2-4.5% by weight %.

Preferably, the emulsifier is polyvinyl alcohol.

Preferably, the alcohol is ethylene glycol.

Preferably, the solvent is water.

According to a preferred embodiment of the present invention, the suspension contains odor molecules, polyvinyl alcohol, ethylene glycol and water, wherein the content of the odor molecules is 0.01-50% by weight, the ethylene glycol The content of the polyvinyl alcohol is 2-3% by weight, and the content of the polyvinyl alcohol is 0.25-5% by weight. In this preferred case, the prepared core-shell structure is more stable for storage in an atmospheric environment, and the release of odor molecules is also more stable and easier to control.

According to the present invention, the types of odor molecules are not limited, and can be any substance with odor, for example, but not limited to alcohol, aldehyde, ester, protein, etc., more specifically, can be various flavors , such as but not limited to one or more of milk flavor, longan flavor, vanilla flavor, cola flavor, apple flavor, cumin flavor, lemon flavor, walnut flavor, grape flavor, almond flavor, rose fragrance and ammonia water. Those skilled in the art can choose according to actual needs. In addition, the core-shell structure of the present invention may contain a single odorant molecule, or may contain different odorant molecules, and the different odorant molecules may be a mixture of different odorant molecules in one core-shell structure, or a combination of multiple odorant molecules. A single odor molecule is arranged in different core-shell structures, and different odors can be released according to requirements.

According to the present invention, the substrate can be various substrates, can be rigid substrates, or can be flexible substrates, and the rigid substrates can be but not limited to silicon wafers, glass wafers, quartz wafers, iron wafers, copper wafers, and aluminum wafers. and one of alumina sheets; the flexible substrate can be but not limited to one of PDMS film, PET film, PS film, PU film and PI film.

According to the present invention, the shape of the core-shell structure can be specifically varied according to the surface tension of the suspension and the type of the substrate, and is generally hemispherical. Preferably, the particle size of the core-shell structure is 0.5-1.5 mm.

According to the present invention, preferably, in the core-shell structure, the particle size of the core layer is 0.8-1.0 mm, and the thickness of the shell layer is 0.10-0.15 mm.

According to the present invention, the pattern in the patterned microarray can be any pattern, and can be designed according to specific conditions and actual preferences. According to a specific embodiment of the present invention, the pattern is a lattice pattern of a core-shell structure. Preferably, the lattice pattern is a periodic single-point array. More preferably, the distance between the center lines of two adjacent core-shell structures is 0.5-1.5 cm.

Wherein, the solvent in the solution containing poly(lactic acid-co-glycolic acid) can be any solvent capable of dissolving poly(lactic acid-co-glycolic acid), but since the subsequent drying procedure is involved, therefore, the The solvent is preferably an easily volatile solvent. According to a preferred embodiment of the present invention, the solvent is dichloromethane.

According to the present invention, in the solution containing poly(lactic acid-co-glycolic acid), the concentration of poly(lactic acid-co-glycolic acid) is not particularly limited, considering the stability of the formed core-shell structure Preferably, the concentration of the poly(lactic-co-glycolic acid) is 2-3% by weight.

According to the invention, the printing is preferably ink jet printing. Specifically, the suspension containing the odor molecules is loaded into the ink cartridge of the inkjet printer as ink, and a printing nozzle with a suitable inner diameter (for example, 0.06mm) is selected to print according to a preset pattern, and then the poly(lactic acid-co-co - glycolic acid) solution is loaded into the ink cartridge of the inkjet printer, in-situ printing is carried out according to the preset pattern, and it is covered on the odor molecular ink droplets, and the solvent in the poly(lactic acid-co-glycolic acid) solution is volatilized to separate out the PLGA solid, that is, The core-shell structure can be obtained.

Among them, the term "in situ printing" is to use the same pattern to coat poly(lactic-co-glycolic acid) on the odor molecular suspension pattern to form a core-shell protective structure.

In a third aspect, the present invention provides a patterned microarray prepared by the method as described above.

In a fourth aspect, the present invention provides the use of patterned microarrays as described above in the manufacture of products for odor-on-demand release, olfactory sensitivity detection and olfactory sensitivity exercise.

The present invention will be described in detail below by means of examples.

Example 1

This example is used to illustrate the patterned microarray of odor molecules provided by the present invention and its preparation

Dissolve 5g of longan essence solution (water-based fragrance), 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10wt%) in 100g of water and mix to form a suspension containing odor molecules. Among them, a printing nozzle with an inner diameter of 0.06 mm was selected to print a 9×4 dot matrix droplet pattern on the PET film substrate according to the preset pattern. The poly(lactic acid-co-glycolic acid) (PLGA, 2.5wt%, the solvent is dichloromethane) is loaded into the inkjet printer cartridge, the printing nozzle with the inner diameter of 0.06mm is selected, and the in-situ printing is carried out according to the preset pattern, covering the fragrance. On the molecular ink droplets, the PLGA solid is formed by the volatilization of dichloromethane to form a core-shell structure (the core-shell structure is a hemisphere with a particle size of 0.9-1.15 mm; in the core-shell structure, the particle size of the core layer is is 0.8-1.0mm, and the thickness of the shell is 0.1-0.15mm), as shown in Figure 2 and Figure 3. By scratching different numbers of scent balls, different concentrations of longan aroma can be smelled.

Example 2

This example is used to illustrate the patterned microarray of odor molecules provided by the present invention and its preparation

Mix 5g of milk flavor solution (water-based flavor), 2g of ethylene glycol and 2g of polyvinyl alcohol (PVA, 10wt%) into a suspension containing odor molecules. After configuration, put it into the ink cartridge of the inkjet printer, and select an inner diameter of 0.06mm. The printing nozzle prints a 9×4 dot matrix droplet pattern on the PET film substrate according to the preset pattern. The poly(lactic acid-co-glycolic acid) (PLGA, 2wt%, the solvent is dichloromethane) was loaded into an inkjet printer cartridge, and a printing nozzle with an inner diameter of 0.06 mm was selected to perform in-situ printing according to a preset pattern, covering the fragrance molecules. On the ink droplets, the PLGA solid is formed when the dichloromethane is volatilized to form a core-shell structure (the core-shell structure is a hemisphere with a particle size of 0.9-1.15 mm; in the core-shell structure, the particle size of the core layer is 0.8-1.0mm, the thickness of the shell is 0.1-0.15mm), as shown in Figure 3. By scratching different numbers of scent balls, different concentrations of milk aroma can be smelled.

Example 3

This example is used to illustrate the patterned microarray of odor molecules provided by the present invention and its preparation

Mix 5g vanilla flavor solution (oily flavor), 3g ethylene glycol and 3g polyvinyl alcohol (PVA, 10wt%) to form a suspension containing odor molecules. After configuration, put it into the ink cartridge of the inkjet printer, and select an inner diameter of 0.06mm. The printing nozzle prints a 9×4 dot matrix droplet pattern on the PET film substrate according to the preset pattern. The poly(lactic acid-co-glycolic acid) (PLGA, 3wt%, the solvent is dichloromethane) was loaded into an inkjet printer cartridge, and a printing nozzle with an inner diameter of 0.06 mm was selected to perform in-situ printing according to a preset pattern, covering the fragrance molecules. On the ink droplets, the PLGA solid is formed when the dichloromethane is volatilized to form a core-shell structure (the core-shell structure is a hemisphere with a particle size of 0.9-1.15 mm; in the core-shell structure, the particle size of the core layer is 0.8-1.0mm, the thickness of the shell is 0.1-0.15mm), as shown in Figure 3. By scratching different numbers of scent balls, different concentrations of vanilla scent can be smelled.

Example 4

This example is used to illustrate the patterned microarray of odor molecules provided by the present invention and its preparation

和

的点阵液滴图案。将聚(乳酸-共-乙醇酸)(PLGA，2.5wt％，溶剂为二氯甲烷)装入喷墨打印机墨盒，选择0.06mm内径的打印喷头，根据预设图案进行原位打印，覆盖于香料分子墨滴之上，待二氯甲烷挥发析出PLGA固体，形成核壳型结构(所述核壳结构为粒径0.9-1.15mm的半球形；在所述核壳结构中，核层的粒径为0.8-1.0mm，壳层的厚度为0.1-0.15mm)，如图4所示。通过刮擦不同数量的气味球，可以闻到不同浓度的桂圆香味。Mix 5g of longan essence solution (water-based fragrance), 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10wt%) into a suspension containing odor molecules. After configuration, put it into the ink cartridge of the inkjet printer, select 0.06 Printing nozzles with an inner diameter of mm, respectively print on the PET film substrate according to the preset pattern

and

dot matrix droplet pattern. The poly(lactic acid-co-glycolic acid) (PLGA, 2.5wt%, the solvent is dichloromethane) is loaded into the inkjet printer cartridge, the printing nozzle with the inner diameter of 0.06mm is selected, and the in-situ printing is carried out according to the preset pattern, covering the fragrance. On the molecular ink droplets, the PLGA solid is formed by the volatilization of dichloromethane to form a core-shell structure (the core-shell structure is a hemisphere with a particle size of 0.9-1.15 mm; in the core-shell structure, the particle size of the core layer is is 0.8-1.0mm, and the thickness of the shell is 0.1-0.15mm), as shown in Figure 4. By scratching different numbers of scent balls, different concentrations of longan aroma can be smelled.

Example 5

This example is used to illustrate the patterned microarray of odor molecules provided by the present invention and its preparation

Mix 5g of longan essence solution (water-based fragrance), 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10wt%) into a suspension containing odor molecules. After configuration, put it into the ink cartridge of the inkjet printer, select 0.06 The printing nozzle with an inner diameter of mm prints a "milk" dot matrix droplet pattern on the PET film substrate according to the preset pattern. The poly(lactic acid-co-glycolic acid) (PLGA, 2.5wt%, the solvent is dichloromethane) is loaded into the inkjet printer cartridge, the printing nozzle with the inner diameter of 0.06mm is selected, and the in-situ printing is carried out according to the preset pattern, covering the fragrance. On the molecular ink droplets, the PLGA solid is formed by the volatilization of dichloromethane to form a core-shell structure (the core-shell structure is a hemisphere with a particle size of 0.9-1.15 mm; in the core-shell structure, the particle size of the core layer is is 0.8-1.0mm, and the thickness of the shell is 0.1-0.15mm), as shown in Figure 4. By scratching different numbers of scent balls, different concentrations of longan aroma can be smelled.

Example 6

This example is used to illustrate the patterned microarray of odor molecules provided by the present invention and its preparation

Mix 5g of longan essence solution (water-based fragrance), 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10wt%) into a suspension containing odor molecules. After configuration, put it into the ink cartridge of the inkjet printer, select 0.06 The printing nozzle with an inner diameter of mm prints a 9×4 dot matrix droplet pattern on a glass sheet, a silicon wafer (hard material), and a PET film (flexible material) according to the preset pattern. The poly(lactic acid-co-glycolic acid) (PLGA, 2.5wt%, the solvent is dichloromethane) is loaded into the inkjet printer cartridge, the printing nozzle with the inner diameter of 0.06mm is selected, and the in-situ printing is carried out according to the preset pattern, covering the fragrance. On the molecular ink droplets, after the dichloromethane volatilizes and separates out the PLGA solid, the materials of the three substrates can form a core-shell structure (the core-shell structure is a hemisphere with a particle size of 0.9-1.15mm; in the core-shell structure , the particle size of the core layer is 0.8-1.0 mm, and the thickness of the shell layer is 0.1-0.15 mm), as shown in FIG. 5 . By scratching different numbers of scent balls, different concentrations of longan fragrance can be smelled.

Example 7

This example is used to illustrate the patterned microarray of odor molecules provided by the present invention and its preparation

Mix 5g of 25-28% ammonia solution, 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10wt%) into a suspension containing odor molecules. After configuration, put it into the ink cartridge of the inkjet printer, select 0.06mm The inner diameter printing nozzle prints a 9×4 dot matrix droplet pattern on the PET film substrate according to the preset pattern. The poly(lactic acid-co-glycolic acid) (PLGA, 2.5wt%, the solvent is dichloromethane) is loaded into the inkjet printer cartridge, the printing nozzle with the inner diameter of 0.06mm is selected, and the in-situ printing is carried out according to the preset pattern, covering the fragrance. On the molecular ink droplets, the PLGA solid is formed by the volatilization of dichloromethane to form a core-shell structure (the core-shell structure is a hemisphere with a particle size of 0.9-1.15 mm; in the core-shell structure, the particle size of the core layer is is 0.8-1.0mm, and the thickness of the shell is 0.1-0.15mm). Prepare precision test paper with pH 8.2-10.0, moisten it with secondary water (pH=5.9) for later use; after scratching different numbers of odor balls, use the wet test paper to detect the odor emitted, as shown in Figure 6, It can be seen that the color of the test paper gradually turns blue as the amount of scratching the odor ball increases.

Example 8

This example is used to illustrate the patterned microarray of odor molecules provided by the present invention and its preparation

Mix 5g of rose essence solution (oily spice), 2.5g of ethylene glycol and 4.5g of polyvinyl alcohol (PVA, 10wt%) into a suspension containing odor molecules. After configuration, put it into the ink cartridge of the inkjet printer, select 0.06 The printing nozzle with an inner diameter of mm prints a 9×4 dot matrix droplet pattern on the PET film according to the preset pattern. The poly(lactic acid-co-glycolic acid) (PLGA, 2.5wt%, the solvent is dichloromethane) is loaded into the inkjet printer cartridge, the printing nozzle with the inner diameter of 0.06mm is selected, and the in-situ printing is carried out according to the preset pattern, covering the fragrance. On the molecular ink droplets, after the dichloromethane volatilizes and separates out the PLGA solid, the materials of the three substrates can form a core-shell structure (the core-shell structure is a hemisphere with a particle size of 0.9-1.15mm; in the core-shell structure , the particle size of the core layer is 0.8-1.0 mm, and the thickness of the shell layer is 0.1-0.15 mm). Different concentrations of rose scent can be smelled by scratching different numbers of scent balls.

Example 9

This example is used to illustrate the patterned microarray of odor molecules provided by the present invention and its preparation

The patterned microarrays of odor molecules were prepared according to the method of Example 1, except that the concentration of poly(lactic-co-glycolic acid) was 0.5wt%, 1wt%, 1.5wt%, 2wt%, 2.25wt%, respectively. wt%, 2.5wt%, 3wt%, 5wt%.

Example 10

This example is used to illustrate the patterned microarray of odor molecules provided by the present invention and its preparation

The patterned microarrays of odor molecules were prepared according to the method of Example 1, except that,

Comparative ratio

This comparative example is used to illustrate the reference odor molecule product and its preparation

Referring to the components and proportions of Example 1, the difference is that the traditional fragrance test paper preparation method is used, and the fragrance is directly sprayed on the A4 printing paper, and the fragrance is not obvious within 2 days.

Test Example 1

Prepare KMnO ₄ solution (6.13× ^10-5 mg/mL, several portions, 15 mL each, placed in a petri dish for use), take the samples prepared in the above examples for testing, each sample is tested by scraping a different amount After the odor spheres were scraped, they were placed in KMnO ₄ solution. As the amount of the microarrays prepared in Examples 1-8 increased, the solution changed from purple to orange-red, then orange, and finally yellow. It can be seen that the unscratched odor ball has stable odor and does not leak, as shown in Figure 7.

In Example 9, when the concentration of poly(lactic acid-co-glycolic acid) is lower than 2wt%, although the core-shell coating structure can be formed, the substances in the core will leak to different degrees; when the concentration is higher than 3wt% Although the core-shell coating structure can be formed, it is difficult to release substances in the core in the later stage; when the concentration is between 2-3wt%, the solution will change from purple to orange-red, and then to red as the amount of scratched odor globules increases. Orange and finally yellow.

Test case 2

Prepare KMnO ₄ solution (6.13×10 ^-5 mg/mL, several portions, 15 mL each, placed in a petri dish for use), take the samples prepared in Examples 1-9 for testing, put them in KMnO Leave for 24 hours. After 24 hours, the color of the solutions of Examples 1-8 did not change (as shown in Figure 7), and it was known that the odor balls that were not scratched were stable and did not leak. In addition, take the samples of Examples 1-9, store them in the air, and scrape them off after one month. The samples of Examples 1-8 still have a strong fragrance, and as mentioned above, the taste of the comparative example is not obvious after 2 days of trial production. .

In Example 9, when the concentration of poly(lactic acid-co-glycolic acid) was lower than 2wt%, although a core-shell coating structure could be formed, the substance in the core would leak to varying degrees, and the color of the solution would change; When the concentration is between 2-5wt%, the color of the solution does not change. After being stored for one month, the samples with a concentration of 2-3% by weight of poly(lactic acid-co-glycolic acid) still have a strong fragrance after scraping off, and other smells are light.

The preferred embodiments of the present invention have been described above in detail, however, the present invention is not limited thereto. Within the scope of the technical concept of the present invention, a variety of simple modifications can be made to the technical solutions of the present invention, including the combination of various technical features in any other suitable manner. These simple modifications and combinations should also be regarded as the content disclosed in the present invention. All belong to the protection scope of the present invention.

Claims (10)

1. A patterned microarray of odor molecules, characterized in that, the patterned microarray comprises a substrate and a core-shell structure formed on the substrate, wherein the core-shell structure takes the odor molecule as a core, and uses poly(lactic acid) as a core. -co-glycolic acid) as the shell. 2. The microarray according to claim 1, wherein the core-shell structure is a hemisphere with a particle diameter of 0.5-1.5 mm; Preferably, in the core-shell structure, the particle size of the core layer is 0.8-1.0 mm, and the thickness of the shell layer is 0.10-0.15 mm. 3. The patterned microarray according to claim 1 or 2, wherein the odorant molecule is selected from the group consisting of alcohol, aldehyde, ester, protein, milk flavor, longan flavor, vanilla flavor, cola flavor, apple flavor, cumin flavor , one or more of lemon essence, walnut essence, grape essence, almond essence, rose perfume and ammonia water; and/or The substrate is selected from one of silicon wafers, glass wafers, quartz wafers, iron wafers, copper wafers, aluminum wafers, alumina wafers, PDMS films, PET films, PS films, PU films and PI films. 4. The patterned microarray according to any one of claims 1-3, wherein the patterned microarray is a lattice pattern of the core-shell structure; Preferably, the lattice pattern is a periodic single-dot array. 5. A method for preparing a patterned microarray of odor molecules, characterized in that the method comprises: (1) printing the suspension containing odor molecules on the substrate according to a predetermined pattern; (2) The poly(lactic acid-co-glycolic acid) solution is in-situ printed on the suspension of the odor molecules printed on the substrate, and after the solvent in the poly(lactic acid-co-glycolic acid) solution is volatilized to obtain a A patterned microarray of a core-shell structure with odor molecules as the core and poly(lactic-co-glycolic acid) as the shell arranged in a predetermined pattern. 6. The method of claim 5, wherein the suspension containing odor molecules consists of odor molecules, an emulsifier, an alcohol, and a solvent; Preferably, in the suspension containing odor molecules, the content of the odor molecules is 0.01-50% by weight, the content of the alcohol is 2-3% by weight, and the content of the emulsifier is 0.25-5% by weight %; Preferably, the emulsifier is polyvinyl alcohol; Preferably, the solvent is water; Preferably, the alcohol is ethylene glycol. 7. The method according to claim 5 or 6, wherein the printing in step (1) and step (2) are each independently inkjet printing. 8. The method of claim 5, wherein the poly(lactic-co-glycolic acid) solution is a dichloromethane solution of poly(lactic-co-glycolic acid); Preferably, the concentration of poly(lactic acid-co-glycolic acid) in the poly(lactic acid-co-glycolic acid) solution is 2-3% by weight. 9. A patterned microarray prepared by the method of any one of claims 5-8. 10. Use of the patterned microarray of any one of claims 1-4 and 9 in the manufacture of a product for odor-on-demand release, olfactory sensitivity detection and olfactory sensitivity exercise.

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