CN111908798A - Sr/Mg/Zn/Cu doped silicon-based sol-gel bioactive glass powder and preparation method and application thereof - Google Patents

Abstract

The invention discloses Sr/Mg/Zn/Cu doped silicon-based sol-gel bioactive glass powderAnd a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing the catalyst with water, adding ethyl orthosilicate, triethyl phosphate, calcium nitrate tetrahydrate and Sr²⁺、Mg²⁺、Zn²⁺And Cu²⁺Stirring and hydrolyzing one or more ionic salts to obtain ion-doped silicon-based bioactive glass sol; (2) standing, aging and drying the obtained ion-doped silicon-based bioactive glass sol to obtain an ion-doped silicon-based sol-gel bioactive glass xerogel precursor; (3) and carrying out heat treatment on the obtained bioactive glass xerogel precursor to obtain bioactive glass powder. The bioactive glass powder can meet different clinical special repair effects, and has wide application prospects in bone tissue repair, oral repair, skin repair and body immune regulation.

Description

A silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu and Its preparation method and application

technical field

The invention belongs to the field of bioactive glass, and in particular relates to a silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu and a preparation method and application thereof.

Background technique

Bioactive Glass (BG) was first developed by the American scientist Larry Hench's research group in 1969. It has good biocompatibility, the ability to promote osteoblast proliferation and differentiation, and gene activation. Biomaterials with tissue repair properties. Recent studies have shown that bioactive glass can not only promote the repair of hard tissues such as bones and teeth, but also integrate well with soft tissues to promote the regeneration and repair of soft tissues such as skin.

The sol-gel method is a common technique for preparing nanomaterials. The reaction of sol-gel technology to prepare bioactive glass is completed at room temperature, the calcination temperature is low, the preparation conditions are easy to achieve, and its composition changes can be finely and uniformly regulated, and it can be adjusted at the molecular level to control the degradation rate. The unity of biological activity and cellular activity. The bioactive glass prepared by the sol-gel method has a micro-nano porous structure and a large specific surface area, which is conducive to the ion dissolution of the material, enables the material to exchange ions with the external environment, achieves controllable degradation of the material, and achieves rapid biomineralization. Helps to promote tissue regeneration and repair. After being implanted into the body, the material forms hydroxycarbonated apatite on the surface by virtue of its own surface area and mesoporous pores, which chemically binds with bone tissue, and adsorbs related proteins to synthesize collagen fibers, which promotes the adhesion, differentiation and development of osteoprogenitor cells. Metabolism of the extracellular matrix of osteocytes. On the other hand, along with the material degradation process, ions from the bioactive glass such as silicon, calcium, phosphorus and other ionized lysates activate the expression of osteogenesis-related genes, activate osteogenesis-related signaling pathways, and regulate the adhesion, proliferation and proliferation of osteogenesis-related cells. Differentiation, regulate the process of bone defect repair at the molecular level, and promote the regeneration of bone tissue. Compared with the modification methods such as small molecule drug loading and RNA grafting, the introduction of trace ions with specific repair effects into the glass network requires only trace ion complexes to be added in the synthesis preparation, which simplifies the preparation process. The network structure is relatively stable and is less affected by temperature and humidity. The introduced ions can be slowly released as the bioactive glass degrades, absorbed and utilized by the human body, participating in the formation of new tissues and exerting its biological effects.

Strontium (Strontium, Sr) is an essential trace element for the human body, which is involved in maintaining normal functions in the body, and has the effect of preventing caries and enhancing bone strength. Low-dose Sr can effectively treat osteoporosis, and the current drug strontium ranelate has a good effect on the treatment of osteoporosis. Studies have found that a certain amount of Sr can promote the proliferation and differentiation of osteoblasts, and at the same time prevent the resorption of bone tissue by inhibiting the formation of osteoclasts, thereby promoting the growth of new bone.

Magnesium (Mg) is an important element in bone metabolism, affecting osteoblast and osteoclast cell activity and bone growth, especially stimulating osteoblast differentiation in the early stages of osteogenesis. Mg deficiency affects the entire process of bone metabolism, resulting in restricted bone growth, decreased osteoblast activity, osteoporosis, and brittle bone disease.

Zinc (Zn) element plays an important role in maintaining various functions of the human body. It participates in the synthesis of DNA and protein, and stimulates bone formation through protein synthesis. It can stimulate osteoblasts to release alkaline phosphatase and enhance osteogenesis-related genes. expression and collagen synthesis to achieve synergistic osteogenesis. Zn has a direct effect on skeletal development, and Zn deficiency in fetuses and early childhood can cause abnormal skeletal development. Studies have shown that Zn can promote bone formation, accelerate bone protein synthesis, increase the concentration of Ca in bone and the content of alkaline phosphatase, and increase the amount of collagen in bone tissue. An appropriate amount of Zn can also promote the proliferation and differentiation of osteoblasts. And effectively inhibit the bone resorption of osteoclasts.

Copper (Cu) element can induce human bone marrow mesenchymal cells to release hypoxia-inducible factor and up-regulate vascular endothelial growth factor, which contributes to the revascularization of bone defect areas, and the formation of new blood vessels is conducive to the repair and regeneration of bone tissue. Studies have shown that low concentrations of copper and silicon can synergistically stimulate revascularization, and the formation of new blood vessels is beneficial to the repair and regeneration of bone tissue.

Therefore, the present invention organically combines the diversity of components and the special clinical repairing effect of ions with the sol-gel bioactive glass, so as to solve the problem of uneven components of the ion-doped bioactive glass, poor mineralization effect, and in vitro hydroxyapatite Due to the problem of unsatisfactory stone formation ability, silicon-based sol-gel bioactive glass powders doped with Sr/Mg/Zn/Cu components with different therapeutic effects were obtained.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu and its preparation method and application in view of the deficiencies of the prior art, and the preparation method can obtain components Uniform, nano-porous and high specific surface area, excellent hydroxyapatite forming ability, bioactive tissue repair material containing specific therapeutic ions, and simple and convenient preparation method and high conversion efficiency, for sol-gel method bioactive glass related products Development is important.

In order to achieve the above objects, the technical solutions of the present invention are as follows.

A preparation method of silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu, comprising the following steps:

(1) The catalyst and water are prepared into a mixed solution, stirred until completely dissolved to obtain a catalyst solution, and then ethyl orthosilicate, triethyl phosphate, calcium nitrate tetrahydrate and ionic salt are added successively, and the ionic doping is obtained by fully stirring and hydrolyzing The silicon-based bioactive glass sol; the ionic salt is an inorganic salt or related alkoxide containing one or more of Sr ²⁺ , Mg ²⁺ , Zn ²⁺ and Cu ²⁺ ;

(2) The ion-doped silicon-based bioactive glass sol obtained in step (1) is allowed to stand and age, so that the hydrolysis and polycondensation reaction can be fully carried out to form a wet gel, which is then placed in a drying oven for gradient drying to obtain the ion-doped glass sol. Silica-based sol-gel bioactive glass xerogel precursor;

(3) placing the bioactive glass xerogel precursor obtained in step (2) in a program-controlled high-temperature resistance furnace for heat treatment, wet grinding and sieving to obtain a doped Sr/Mg/Zn/Cu with nanopores Silica-based sol-gel bioactive glass powder.

Preferably, the molar ratio of catalyst: water: ethyl orthosilicate in step (1) is (2-8): 1000: (70-140).

Preferably, the catalyst in step (1) is hydrochloric acid or ammonia water.

Preferably, the molar ratio of ethyl orthosilicate, triethyl phosphate, calcium nitrate tetrahydrate and ionic salt in step (1) is ethyl orthosilicate: triethyl phosphate; calcium nitrate tetrahydrate: ionic salt =(50~80):(14~4):(5~40):(0~15), according to the molar ratio of ethyl orthosilicate, triethyl phosphate, calcium nitrate tetrahydrate and ionic inorganic salt, The molar ratio of silica, phosphorus pentoxide, calcium oxide and inorganic salt in the finally obtained silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu with nanopores=(40～ 70): (36-16): (1-8): (0-15).

Preferably, the ionic inorganic salts in step (1) are chloride salts and their hydrates or nitrates and their hydrates or their related alkoxides, which can be selected from zinc nitrate, copper chloride, magnesium nitrate, Silver nitrate, strontium nitrate, cobalt nitrate, ferric nitrate, copper nitrate, strontium chloride, magnesium methoxide, zinc acetate, etc., including but not limited to the aforementioned substances.

Preferably, the hydrolysis and stirring time in step (1) is 2 to 8 hours.

Preferably, the aging temperature in step (2) is 15-35° C., the aging time is 2-5 days, and the sol is left to stand for aging to undergo hydrolysis and polycondensation reaction to obtain wet gel.

Preferably, in step (2), the gradient temperature of the drying oven is set to be 50-75°C and 100-140°C, and the drying time is 2-9 days to obtain a uniform large granular xerogel precursor.

Preferably, the high-temperature sintering temperature in step (3) is 550-850° C., and the sintering holding time is 1-4 hours.

Preferably, in the wet ball milling described in step (3), the ball milling tank is made of polytetrafluoroethylene, the ball milling time is 3 to 10 hours, the ball milling medium is anhydrous ethanol, the grinding body is a quartz ball, and the mass ratio of the ball milling material is It is a bioactive glass powder: grinding body: ball milling medium = (1~5): (2~10): (1~2); after ball milling, the ball milling medium is removed by drying and sieving to obtain a dopant with uniform particles and nano pores Silica-based sol-gel bioactive glass powders of Sr/Mg/Zn/Cu.

A silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu obtained by the above-mentioned preparation method, the silicon-based sol-gel doped with Sr/Mg/Zn/Cu The particle size distribution of the bioactive glass powder is 10-900 μm, and the specific surface area is 100-300 m ² /g.

The silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu described above can stably release the silicon, calcium, phosphorus plasma dissolved products of the bioactive glass body and its doped Sr ²⁺ and Or Mg ²⁺ and or Zn ²⁺ and or Cu ²⁺ plasma, good in vitro hydroxyapatite formation ability and promote cell growth, ions meet different clinical special repair effects, in bone tissue repair, oral repair, skin repair and body repair It has broad application prospects in immune regulation.

The invention adopts a new generation sol-gel method to prepare silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu, and introduces specific therapeutic elements strontium/magnesium/zinc in the preparation process of the bioactive glass /Cu, to achieve excellent tissue repair effect; the microstructure and biocompatibility of bioactive glass are not changed after doping with Sr/Mg/Zn/Cu.

Compared with the prior art, the present invention has the following advantages:

In the present invention, two materials of bioactive glass and therapeutic elements strontium/magnesium/zinc/copper are selected for compounding, and ions are added to the synthesis of bioactive glass in the form of inorganic salts. The silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu has uniform composition, excellent hydroxyapatite formation ability in vitro, and stable release of ions for specific tissue repair effects; appropriate amount of element doping, in vitro The mineralization effect is good, and the biological activity of the material is not changed; the synthetic material has a wide range of sources and low price; the preparation method is simple and convenient, and the conversion efficiency is high, which is of great significance for the development of sol-gel bioactive glass related products.

Description of drawings

FIG. 1 is a flow chart of the preparation process of the silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu according to the present invention.

2 is a scanning electron microscope image of the silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu prepared in Example 1 at a magnification of 101 times.

3 is a scanning electron microscope image of the silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu prepared in Example 1 at a magnification of 100,000 times.

4 is a graph showing the in vitro mineralization properties of the Zn/Cu-doped silicon-based sol-gel bioactive glass powder prepared in Example 2.

5 is an analysis diagram of the surface pore structure of the silicon-based sol-gel bioactive glass powder doped with Mg/Zn/Cu prepared in Example 3. FIG.

6 is an X-ray energy dispersive spectroscopy (EDS) image of the Sr-doped silicon-based sol-gel bioactive glass powder prepared in Example 4.

FIG. 7 is an X-ray photoelectron spectroscopy (XPS) image of the Sr-doped silicon-based sol-gel bioactive glass powder prepared in Example 4. FIG.

FIG. 8 is the analysis result of cell proliferation activity of the Sr-doped silicon-based sol-gel bioactive glass powder prepared in Example 4. FIG.

Detailed ways

The present invention will be described in further detail below with reference to the embodiments and the accompanying drawings, but the embodiments of the present invention are not limited thereto.

The flow chart of the preparation process of the silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu in the following embodiments is shown in FIG. 1 .

Example 1

A silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu, the preparation method thereof is as follows:

(1) Preparation steps of bioactive glass sol containing Sr/Mg/Zn/Cu:

26ml of hydrochloric acid with a concentration of 2mol/L and 78ml of deionized water were prepared into a mixed solution, stirred for 10min until completely dissolved, to obtain a catalyst solution, and then 110.1g of ethyl orthosilicate, 10.9g of triethyl phosphate, 58.6g of tetraethyl phosphate were added in sequence. Water calcium nitrate, 5.2g strontium nitrate, 3g magnesium nitrate, 4.4g copper nitrate, 4.4g zinc nitrate, fully stirred for 4h and hydrolyzed to obtain a transparent and uniform bioactive glass sol containing 3Sr-1Mg-1Zn-1Cu (mol%);

(2) Preparation steps of bioactive glass xerogel precursor containing Sr/Mg/Zn/Cu:

The bioactive glass sol obtained in step (1) was allowed to stand at 35°C for 2 days, so that the hydrolysis and polycondensation reaction was fully carried out to form a wet gel, which was then placed in a drying oven for gradient drying, dried at 50°C for 4 days and dried at 135°C. After 3 days, the precursor of Sr/Mg/Zn/Cu bioactive glass xerogel was obtained;

(3) Sintering the Sr/Mg/Zn/Cu-doped bioactive glass xerogel precursor:

The bioactive glass xerogel precursor obtained in step (2) is placed in a high-temperature resistance furnace for heat treatment and sintering at 600° C. for 4 hours, followed by wet grinding for 3 hours and sieving to obtain the doped Sr/Mg/Zn with nano-pore structure. /Cu silica-based sol-gel bioactive glass powder.

The process flow diagram of the preparation method of the Sr/Mg/Zn/Cu doped silicon-based sol-gel bioactive glass powder in this embodiment is shown in FIG. 1 , and FIG. 2 and FIG. 3 The SEM images of the doped Sr/Mg/Zn/Cu silicon-based sol-gel bioactive glass powder with magnifications of 101 times and 100,000 times can be seen from Figures 2 and 3 that the doped Sr/ The silicon-based sol-gel bioactive glass powder of Mg/Zn/Cu is composed of a large number of uniform nano-scale microspheres, and there are tiny nano-pores between the particles. Doping ions does not change the surface morphology of the bioactive glass. According to the laser particle size analyzer and BET multi-point method, the particle size distribution of the silicon-based sol-gel bioactive glass powder doped with Sr/Mg/Zn/Cu in this embodiment is 10-810 μm, and the specific surface area is 204.9 m ² /g.

Example 2

A silicon-based sol-gel bioactive glass powder doped with Zn/Cu, the preparation method thereof is as follows:

(1) Preparation steps of bioactive glass sol containing Zn/Cu:

26ml of hydrochloric acid with a concentration of 2mol/L and 78ml of deionized water were prepared into a mixed solution, stirred for 10min until completely dissolved, to obtain a catalyst solution, and then 110.1g of ethyl orthosilicate, 10.9g of triethyl phosphate, 58.6g of tetraethyl phosphate were added in sequence. Water calcium nitrate, 13.2g copper nitrate, 13.2g zinc nitrate, fully stirred for 4h and hydrolyzed to obtain a transparent and uniform bioactive glass sol containing 3Zn-3Cu (mol%);

(2) Preparation steps of Zn/Cu-containing bioactive glass xerogel precursor:

The bioactive glass sol obtained in step (1) was left to age at 25°C for 3 days, so that the hydrolysis and polycondensation reaction was fully carried out to form a wet gel, which was then placed in a drying oven for gradient drying, dried at 60°C for 3 days and dried at 140°C. After 2 days, the Zn-Cu bioactive glass xerogel precursor was obtained;

(3) Sintering the Zn/Cu-doped bioactive glass xerogel precursor:

The bioactive glass xerogel precursor obtained in step (2) is placed in a high-temperature resistance furnace for heat treatment and sintering at 600° C. for 4 hours, wet grinding for 3 hours, and sieved to obtain the Zn/Cu doped silicon with nano-pore structure. Based sol-gel bioactive glass powder.

The Zn/Cu-doped silicon-based sol-gel bioactive glass powders prepared in this example were soaked in simulated body fluids for 5 days according to the standard ISO/FDIS 23317:2007(E) to characterize their in vitro mineralization properties. Scanning electron microscopy The results of the figure (as shown in Figure 4) show that the material can generate needle-like hydroxyapatite with good crystallinity in a short time, indicating that the silicon-based sol-gel bioactive glass powder doped with Zn/Cu prepared in this example It has good hydroxyapatite forming ability and excellent mineralization effect. According to the laser particle size analyzer and the BET multi-point method, the particle size distribution of the Zn/Cu-doped silicon-based sol-gel bioactive glass powder in this embodiment is calculated to be 15-850 μm, and the specific surface area is 125.5 m ² /g.

Example 3

A silicon-based sol-gel bioactive glass powder doped with Mg/Zn/Cu, the preparation method thereof is as follows:

(1) Preparation steps of bioactive glass sol containing Mg/Zn/Cu:

26ml of hydrochloric acid with a concentration of 2mol/L and 78ml of deionized water were prepared into a mixed solution, stirred for 10min until completely dissolved, to obtain a catalyst solution, and then 110.1g of ethyl orthosilicate, 10.9g of triethyl phosphate, 58.6g of tetraethyl phosphate were added in sequence. Water calcium nitrate, 6g magnesium nitrate, 8.8g copper nitrate, 8.8g zinc nitrate, fully stirred for 4h and hydrolyzed to obtain a transparent and uniform bioactive glass sol containing 2Mg-2Zn-2Cu (mol%);

(2) Preparation steps of bioactive glass xerogel precursor containing Mg/Zn/Cu:

The bioactive glass sol obtained in step (1) was allowed to stand at 35°C for 2 days, so that the hydrolysis and polycondensation reaction was fully carried out to form a wet gel, which was then placed in a drying oven for gradient drying, dried at 50°C for 4 days and dried at 135°C. After 3 days, the Mg/Zn/Cu bioactive glass xerogel precursor was obtained;

(3) Sintering the Mg/Zn/Cu-doped bioactive glass xerogel precursor:

The bioactive glass xerogel precursor obtained in step (2) is placed in a high-temperature resistance furnace for heat treatment and sintering at 600° C. for 4 hours, wet-grinding for 3 hours, and sieved to obtain the doped Mg/Zn/Cu with nano-pore structure. Silica-based sol-gel bioactive glass powder.

The specific surface area and particle size of the silicon-based sol-gel bioactive glass powder doped with Mg/Zn/Cu prepared in this example were tested by using a specific surface and pore size analyzer and a laser particle size analyzer. The specific surface area of the material and mesoporous pore size were tested according to the regulations of GB/T 19587-2004 and GB/T 21650.2-2008. The results are shown in Figure 5. It can be seen that the obtained silicon-based sol-gel bioactive glass powder doped with Mg/Zn/Cu is rich in It contains a large number of slit mesopores formed by the accumulation of nanoparticles, the particle size distribution is 10-740μm, the pore size is 6.546nm, and the specific surface area is 161.6m ² /g, which is relatively high.

Example 4

A silicon-based sol-gel bioactive glass powder doped with Sr, its preparation method is as follows:

(1) Preparation steps of Sr-containing bioactive glass sol:

26ml of hydrochloric acid with a concentration of 2mol/L and 78ml of deionized water were prepared into a mixed solution, stirred for 10min until completely dissolved, to obtain a catalyst solution, and then 110.1g of ethyl orthosilicate, 10.9g of triethyl phosphate, 58.6g of tetraethyl phosphate were added in sequence. Water calcium nitrate and 10.4g strontium nitrate, fully stirred for 4h and hydrolyzed to obtain a transparent and uniform bioactive glass sol containing 6Sr (mol%);

(2) Preparation steps of Sr-containing bioactive glass xerogel precursor:

The bioactive glass sol obtained in step (1) was allowed to stand at 35°C for 2 days, so that the hydrolysis and polycondensation reaction was fully carried out to form a wet gel, which was then placed in a drying oven for gradient drying, dried at 50°C for 4 days and dried at 135°C. After 3 days, the Sr-doped bioactive glass xerogel precursor was obtained;

(3) sintering the Sr-doped bioactive glass xerogel precursor:

The bioactive glass xerogel precursor obtained in step (2) is placed in a high temperature resistance furnace for heat treatment and sintering at 700 ° C for 4 hours, wet grinding for 3 hours, and sieving to obtain the Sr-doped silicon-based sol with nano-pore structure. -Gel bioactive glass powder.

The Sr-doped silicon-based sol-gel bioactive glass powder prepared in this example was characterized as follows: X-ray energy spectroscopy (EDS) diagram (see Figure 6), X-ray photoelectron spectroscopy (XPS) diagram ( See Figure 7), and the results of biocompatibility analysis (see Figure 8). It can be seen from FIG. 6 that the Sr-doped silicon-based sol-gel bioactive glass powder obtained in this example can observe obvious characteristic peaks corresponding to Si, O, P, Ca, and Sr. The corresponding molar ratio of each element in the Sr-doped silicon-based sol-gel bioactive glass powder is roughly the molar ratio of silicon dioxide, phosphorus pentoxide, calcium oxide and strontium oxide=53.9:36.7:3.6:5.9; Fig. 7 shows that the Sr-doped silicon-based sol-gel bioactive glass powder obtained in this example has been successfully doped with strontium element; The particle size distribution of the silica-based sol-gel bioactive glass powder is 10-750 μm, and the specific surface area is 125.5 m ² /g. It can be seen from FIG. 8 that the Sr-doped silica-based sol-gel bioactive glass powder obtained in this example was co-cultured with mouse bone marrow mesenchymal stem cells (BMSC) for different days (1, 3, 5 days). ) histogram of CCK-8 cell proliferation results, from left to right are the cell culture solutions of the Sr-doped silica-based sol-gel bioactive glass powder with different concentrations, 1000 μg/mL, 500 μg/mL mL, 200 μg/mL, 20 μg/mL, and blank group (only cells), the abscissa is the material and cell culture time, and the ordinate is the cell viability value. Compared with the blank group, the silicon-based sol-gel bioactive glass powder doped with Sr can promote cell proliferation, indicating that the material has good biocompatibility and promotes cell adhesion and proliferation.

The above-mentioned embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited by the above-mentioned embodiments, and any other changes, modifications, substitutions, combinations, The simplification should be equivalent replacement manners, which are all included in the protection scope of the present invention.

Claims (10)

1. A preparation method of Sr/Mg/Zn/Cu doped silicon-based sol-gel bioactive glass powder is characterized by comprising the following steps:

(1) preparing a mixed solution of a catalyst and water, stirring until the mixed solution is completely dissolved to obtain a catalyst solution, then sequentially adding tetraethoxysilane, triethyl phosphate, calcium nitrate tetrahydrate and an ionic salt, and fully stirring and hydrolyzing to obtain ion-doped silicon-based bioactive glass sol; the ionic salt contains Sr²⁺、Mg²⁺、Zn²⁺And Cu²⁺Inorganic salts or related alkoxides of one or more of (a);

(2) standing and aging the ion-doped silicon-based bioactive glass sol obtained in the step (1) to fully perform a hydrolysis polycondensation reaction to form wet gel, and then placing the wet gel in a drying box for gradient drying to obtain an ion-doped silicon-based sol-gel bioactive glass xerogel precursor;

(3) and (3) placing the bioactive glass xerogel precursor obtained in the step (2) into a program-controlled high-temperature resistance furnace for heat treatment, and obtaining the Sr/Mg/Zn/Cu-doped silicon-based sol-gel bioactive glass powder with nano pores through wet grinding and screening.

2. The method of claim 1, wherein: the catalyst in the step (1): water: the molar ratio of the ethyl orthosilicate is (2-8): (800-1000): (70-140); the catalyst is hydrochloric acid or ammonia water; the stirring hydrolysis time is 2-8 hours.

3. The method of claim 1, wherein: in the step (1), the molar ratio of the ethyl orthosilicate, the triethyl phosphate, the calcium nitrate tetrahydrate and the ionic salt is that the ethyl orthosilicate is as follows: triethyl phosphate; calcium nitrate tetrahydrate: an ionic salt (50-80): (14-4): (5-40): (0-15).

4. The method of claim 1, wherein: the ionic salt in the step (1) is selected from one or more of zinc nitrate, copper chloride, magnesium nitrate, silver nitrate, strontium nitrate, cobalt nitrate, ferric nitrate, copper nitrate, strontium chloride, magnesium methoxide and zinc acetate.

5. The method of claim 1, wherein: the aging temperature in the step (2) is 15-35 ℃, and the aging time is 2-5 days.

6. The method of claim 1, wherein: and (2) gradient drying, namely setting the gradient temperature of a drying box to be 50-75 ℃ and 100-140 ℃, and drying for 2-9 days.

7. The method of claim 1, wherein: the temperature of the heat treatment in the step (3) is 550-850 ℃, and the heat preservation time of the heat treatment is 1-4 hours.

8. The method of claim 1, wherein: and (3) carrying out wet grinding, wherein a ball milling tank is made of polytetrafluoroethylene, the ball milling time is 3-10 hours, and a ball milling medium is absolute ethyl alcohol.

9. The Sr/Mg/Zn/Cu doped silicon-based sol-gel bioactive glass powder prepared by the preparation method of any one of claims 1 to 8 is characterized in that the Sr/Mg/Zn/Cu doped silicon-based sol-gel bioactive glass powder has the particle size distribution of 10 to 900 microns and the specific surface area of 100 to 300m²/g。

10. The use of the Sr/Mg/Zn/Cu doped silica-based sol-gel bioactive glass powder of claim 9 in bone tissue repair, oral repair, skin repair and immune regulation of the body.

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