CN112111402A - Culture system for cell biological electromagnetic effect evaluation - Google Patents

Abstract

The invention belongs to the research field of the biological effects of pulsed electromagnetic fields. The invention discloses a culture system for evaluating the biological electromagnetic effect of cells, which provides a constant temperature and humidity environment for cells, reduces the influence of the external environment on the biological effects of cells, and provides a basis for accurately evaluating the radiation effect of electromagnetic fields on cells and molecules. The system includes: a signal generator, a power amplifier and a cell culture incubator; the cell culture incubator includes a box body, a support, a cell culture plate and a magnetic field detection sensor arranged in the box body; the cell culture plate is arranged on the support, and the cell culture plate There are evenly distributed plate holes for containing cell culture liquid; a layer of array coils is arranged above and below the cell culture plate; the signal generator is electrically connected to the power amplifier, and the power amplifier is electrically connected to the array coil; the magnetic field detection sensor Electrically connected to the signal amplifier.

Description

A culture system for the assessment of biological electromagnetic effects in cells

technical field

The invention relates to a culture system for evaluating the biological electromagnetic effect of cells, and belongs to the field of research on the biological effect of pulsed electromagnetic fields.

Background technique

The biological effects of alternating electromagnetic fields at the cellular level are the basis for the study of biological electromagnetic effects, and have certain guiding significance in the application of electromagnetic fields to the treatment and prevention of diseases.

The current conventional method for evaluating the biological effects of alternating electromagnetic fields on cells is to expose cells to alternating electromagnetic fields in an open environment. In an open environment, temperature, air humidity and pH in the culture medium will cause biological changes in cells to a certain extent, making it difficult to distinguish and study the biological effects of alternating electromagnetic fields on cells; Increased exposure time resulted in decreased cell viability.

SUMMARY OF THE INVENTION

The technical problem to be solved by the present invention is: to propose a culture system for evaluating the biological electromagnetic effect of cells, providing a constant temperature and humidity environment for cells, reducing the influence of the external environment on the biological effect of cells, and accurately evaluating the effect of electromagnetic fields on the cellular and molecular levels. provide the basis for the radiation effect.

The technical scheme adopted by the present invention to solve the above-mentioned technical problems is:

A culturing system for evaluating the electromagnetic effect of cell biology, comprising: a signal generator, a power amplifier and a cell incubator; the cell incubator includes a box and a bracket, a cell culture plate and a magnetic field detection sensor arranged in the box The cell culture plate is arranged on a support, and the cell culture plate is provided with evenly distributed plate holes for containing cell culture liquid; a layer of array coils is arranged above and below the cell culture plate; the signal The generator is electrically connected to the power amplifier for generating a variety of driving currents with different waveforms and with adjustable intensity and frequency; the power amplifier is electrically connected to the array coil for amplifying the driving current to drive the array coil to act on the cells The alternating magnetic field of the culture plate; the magnetic field detection sensor is electrically connected to the signal generator for detecting the actual magnetic field strength in the cell incubator and feeding it back to the signal generator to form a closed-loop control, which is used as the signal generator for the generated magnetic field Intensity adjustment.

As a further optimization, the array coil includes a plurality of circular coils connected in series, the number of circular coils is equal to the number of the plate holes, and the inner diameter of the circular coils is equal to the inner diameter of the plate hole.

As a further optimization, the circular coils in the array coils above and below the cell culture plate are in one-to-one correspondence with the plate wells, and the center of each circular coil is aligned with the center of the corresponding plate well.

As a further optimization, the distances between the array coils above and below the cell culture plate are equal to the cell culture plate, and the interval between the upper array coil and the lower array coil is equal to the radius of the circular coil.

The beneficial effects of the present invention are:

Based on the above-designed culture system, the cell culture plate and the array coil are integrated into the box, and are connected to the external power amplifier with a cable through the sealing port, so as to avoid exposing the cells to the open alternating electromagnetic environment and reduce the The influence of the external environment on the biological effect of cells; and through the arrangement of the upper and lower array coils of the cell culture plate, a Helmholtz coil array is formed, so that the cell culture plate in the middle can obtain a magnetic field environment with better magnetic field uniformity. This ensures the stability and standardization of cell culture, and provides a basis for accurately evaluating the radiation effects of electromagnetic fields on cells and molecules.

Description of drawings

Fig. 1 is the principle block diagram of the culture system in the present invention;

2 is a schematic structural diagram of a culture system in an embodiment of the present invention;

Figure 3 is a schematic diagram of a cell culture plate and the upper and lower array coils;

Figure 4 is a schematic diagram of the electromagnetic field of the plate wells on the cell culture plate;

In the figure, 1 is the box body, 2 is the array coil on the upper layer, 3 is the cell culture plate, 4 is the array coil on the lower layer, 5 is the bracket, and 6 is the power amplifier.

Detailed ways

The invention aims to provide a culture system for evaluating the biological electromagnetic effect of cells, which provides a constant temperature and humidity environment for cells, reduces the influence of the external environment on the biological effect of cells, and provides a basis for accurately evaluating the radiation effect of electromagnetic fields on cells and molecules. . The system includes: a signal generator, a power amplifier, and a cell incubator. The cell culture incubator integrates a cell culture plate and an array coil, and a magnetic field detection sensor is set. The principle is shown in Figure 1. The signal generator generates a specific waveform After the signal is amplified by the power amplifier, the array coil is driven to generate an alternating magnetic field, and the alternating magnetic field acts on the cell culture plate, thereby providing a uniform magnetic field environment for the cells in the plate hole, and the actual magnetic field strength is measured by the alternating magnetic field sensor and feedback For the signal generator, a closed-loop control is formed, so as to control the magnetic field intensity by adjusting the waveform parameters output by the signal generator. Under the condition of ensuring the normal temperature, humidity and pH value of the cells, the culture system uses the magnetic field as a variable variable to evaluate the influence of the electromagnetic field on the cellular molecular level. While minimizing the impact of the external environment on the biological effects of cells, it ensures cell culture. The stability and standardization of electromagnetic fields provide a basis for accurate assessment of the radiation effects of electromagnetic fields on cells and molecules.

Example:

Referring to FIG. 2 , the culture system for evaluating the electromagnetic effect of cells in this embodiment includes: a signal generator, a power amplifier 6 and a cell incubator; the cell incubator includes a box 1 and a A bracket 5, a cell culture plate 3 and a magnetic field detection sensor; the cell culture plate 3 is arranged on the bracket 5, and the cell culture plate 3 is provided with evenly distributed plate holes for containing cell culture liquid; A layer of array coil 3 is arranged above, and a layer of array coil 4 is arranged below, the array coil 3 and the array coil 4 are electrically connected with the power amplifier 6, the power amplifier 6 is electrically connected with the signal generator, and the magnetic field detection sensor It is used to detect the actual magnetic field strength in the cell incubator and feed it back to the signal generator, which is used as the signal generator to adjust the generated magnetic field strength.

Specifically, the signal generator in this embodiment is used to generate specific waveforms, including sine waves, triangle waves, square waves, pulse waves and other waveforms, and set specific waveform parameters (frequency, size, offset, phase, etc.) , the frequency range supports 0Hz-1MHz, which generates the corresponding alternating magnetic field (sinusoidal alternating magnetic field, triangular wave alternating magnetic field, square wave alternating magnetic field, pulse wave alternating magnetic field) through the power amplifier control coil.

The power amplifier in this embodiment is used to amplify the waveform of the signal generator to drive the coil. In the specific selection, a power amplifier with controllable gain can be selected, the harmonic distortion is less than 1%, the driving power is greater than 500w, and the frequency response The range is 0Hz-10khz and the voltage range is 0-220v.

The cell culture incubator can be a carbon dioxide incubator for standardized cell culture, and its environmental parameters are: 37 degrees Celsius, 95% space humidity, 5% carbon dioxide gas; in this embodiment, the cell culture plate and the array coil are integrated and placed in the carbon dioxide incubator, An alternating magnetic field sensor is set in the carbon dioxide incubator; then the array coil is connected to the power amplifier outside the incubator with a cable through the sealing port, and the alternating magnetic field sensor is connected to the signal generator through a signal line.

In this embodiment, there are one layer of array coils above and below the cell culture plate. The array coils are composed of uniformly arranged circular coils. The number of circular coils is the same as the number of plate holes on the cell culture plate. The inner diameter of the coil is the same as the inner diameter of the plate hole, and each circular coil corresponds to one plate hole, that is, when setting, ensure that the center of the plate hole corresponds to the center of the circular coil, and each plate hole will be in the upper and lower circles. in the middle of the coil, thereby improving the magnetic field uniformity of the cells in the wells of the plate, as shown in Figures 3 and 4. In addition, in order to further improve the uniformity of the magnetic field, the cell culture plate and the upper and lower array coils are arranged at equal intervals, and the interval between the upper and lower array coils is guaranteed to be equal to the radius of the circular coil. In this way, the upper and lower array coils form a Helm. According to the characteristics of the Helmholtz coil array, when the upper and lower coils pass the same direction and the same magnitude of current, a relatively wide uniform magnetic field will be generated near the midpoint of the common axis of the two coils. Therefore, the cell culture plate can be in a relatively uniform magnetic field environment.

In actual operation, we can simulate the magnetic field through electromagnetic software, and fine-tune the relative position of the array coil and the cell culture plate according to the simulation results, so as to ensure that the electromagnetic radiation environment on the cell plate is a uniform field.

It should be noted that the claimed scheme of the present invention includes but is not limited to the above-mentioned embodiments. Those skilled in the art can make equivalent modifications/substitutions based on the above-mentioned embodiments without departing from the protection scope of the present invention.

Claims (4)

1. a kind of culture system for the evaluation of cell biological electromagnetic effect, is characterized in that, It includes: a signal generator, a power amplifier and a cell incubator; the cell incubator includes a box and a support, a cell culture plate and a magnetic field detection sensor arranged in the box; the cell culture plate is arranged on the support, and the cell culture The plate is provided with evenly distributed plate holes for containing the cell culture liquid; a layer of array coils is arranged above and below the cell culture plate; the signal generator is electrically connected to the power amplifier for generating a variety of drive currents with different waveforms and adjustable intensity and frequency; the power amplifier is electrically connected to the array coil for amplifying the drive current to drive the array coil to generate an alternating magnetic field acting on the cell culture plate; the magnetic field detection sensor is connected to the array coil. The signal generator is electrically connected to detect the actual magnetic field strength in the cell culture incubator and feed it back to the signal generator to form a closed-loop control, which is used as the signal generator to adjust the generated magnetic field strength. 2. a kind of culture system for cell biological electromagnetic effect assessment as claimed in claim 1, is characterized in that, The array coil includes a plurality of circular coils connected in series, the number of circular coils is equal to the number of the plate holes, and the inner diameter of the circular coils is equal to the inner diameter of the plate holes. 3. a kind of culturing system for cell bio-electromagnetic effect assessment as claimed in claim 2, is characterized in that, The circular coils in the array coils above and below the cell culture plate are in one-to-one correspondence with the plate holes, and the center of each circular coil is aligned with the center of the corresponding plate hole. 4. a kind of culture system for cell biological electromagnetic effect assessment as claimed in claim 3, is characterized in that, The distances between the array coils above and below the cell culture plate and the cell culture plate are equal, and the interval between the upper array coil and the lower array coil is equal to the radius of the circular coil.

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