CN1126441C - Florescence controlling method for rice hybrid production - Google Patents

Abstract

The invention relates to a flowering time regulating method for rice hybrid production, belongs to the application field of jasmonic acid plant hormones, and is specially used for the flowering time regulation of rice sterile line breeding and hybrid production. It is characterized in that: in the breeding of rice sterile lines or rice hybrid seed production, 10-120 minutes before the flowering peak of maintainer lines or restorer lines (or sterile lines), it is effective to use jasmonic acid plant hormones and their analogues. As a component regulator, the sterile line (or restorer line) is sprayed at a dose of 1.5-600 g active ingredient/ha. The advantages of the present invention are: within 10-120 minutes after the implementation of the technology, the sterile line (or restorer line) can be made to flower, and the flowering peak period formed is the same as the flowering peak of the maintainer line or restorer line or sterile line. meet.

Description

A method for regulating flowering time for rice hybrid production

1. Technical field

The invention relates to a flowering time regulation method for rice hybrid production, belongs to the application field of jasmonic acid plant hormones and analogues thereof, and is specially used for the flowering time regulation of rice sterile line breeding and hybrid production.

2. Technical Background

The production of hybrid seeds is a key link in the utilization of rice heterosis, and the flowering time mismatch between sterile lines and maintainer lines or between sterile lines and restorer lines is one of the main limiting factors for improving seed production. Quite a number of CMS lines have the disadvantage of poor flowering habit, that is, late flowering and inconcentrated flowering time, resulting in poor flowering time encounter with maintainer lines or restorer lines, which seriously affects the yield of CMS breeding and hybrid production. Some combinations with strong advantages cannot even be used because the flowering habit of the sterile lines is too poor to obtain F1 seeds. In the inter-subspecies production of the two-line method, if japonica rice is used as the male parent (restorer line), the flowering time of the male parent is later than that of the female parent, and the flowering time of the male parent needs to be advanced. In short, there is an urgent need for a practical and specialized technology to solve the above-mentioned problems, that is, to advance the flowering time of the female parent or the male parent, and make its flowering peak meet the flowering peak of the male or female parent. Although there are some flowering time regulators, such as: CO2, warming agent, Huaxinling, Tiaohuabao, etc., they are either inconvenient to be used in production because they are gases, or they are mainly used to adjust the flowering period. The adjustment of time can only play an auxiliary role, and it is difficult to produce direct and visible effects.

3. Technical issues of invention content

The purpose of the present invention is to provide a fast, sensitive and easy flowering time regulation technology of rice male parent or female parent, so that the sterile line or restorer line will start to flower within 10-120 minutes after the technology is implemented, and form a flowering time. peak period, and make it with the maintainer line (such as the sterile line breeding), the restorer line (such as: "three-line method" seed production, or indica as the male parent "two-line method" seed production) or sterile line ( For example: the flowering peaks of japonica rice as the male parent of the "two-line method" seed production) meet. Thereby, breeders of rice sterile lines and producers of hybrid seeds are directly benefited. Technical solutions

The method for regulating and controlling flowering time for rice hybrid production provided by the present invention is characterized in that:

In the breeding of rice sterile lines, at the heading and flowering stage of sterile lines, jasmonic acid plant hormones, coronatine (Coronatine), linolenic acid, 12-oxophytodienoic acid, yamic acid, and methyl yamate As a regulator of the active ingredient, the sterile line is sprayed at a dose of 0.15-60.0 mg active ingredient/square meter, so that the flowering peak of the sterile line can be compared with the flowering peak of the maintainer line or with the flowering peak after 10-120 minutes. The restorer lines meet at the flowering peak;

Or in the heading and flowering stage of the restorer line, use jasmonic acid plant hormones, coronatine (Coronatine), linolenic acid, 12-oxophytodienoic acid, yamic acid, and methyl yamate as regulators of active ingredients, according to The dose of 0.15-60.0 mg active ingredient/square meter is sprayed on the restorer line, so that the flowering peak of the restorer line can meet the flowering peak of the sterile line after 10-120 minutes.

Among them, rice sterile lines refer to three-line sterile lines and two-line sterile lines. The above-mentioned modulators with jasmonic acid phytohormones, corona toxin, linolenic acid, 12-oxo-phytodienoic acid, yamic acid and methyl yamate as active ingredients, the dosage is 0.15-60.0 mg active ingredient/ Square meters work better. The structural formulas of jasmonic acid phytohormones, corona toxin, linolenic acid, 12-oxo-phytodienoic acid, yamic acid and yamic acid methyl ester are shown in Figure 1.

Jasmonic acid is a new class of plant hormones, and methyl jasmonate is a methyl derivative of jasmonic acid. At present, jasmonic acid, as a class of plant hormones, has been recognized by the international academic community. It is known that jasmonic acid hormones have the effects of inhibiting stem and leaf growth, inhibiting germination, promoting rooting of cuttings, promoting senescence, promoting ethylene production, affecting the activity of certain enzymes, promoting stomatal closure, inhibiting flower bud differentiation, and improving stress resistance. But so far, there is no report of jasmonic acid inducing the opening of rice or other cereal crops. (See the reference "Botany Bulletin" 1996.13 (addition): 39-45)

After years of exploration, the inventor found that jasmonic acid phytohormones can quickly and strongly promote the flowering of rice, and further studied their physiological mechanism for the opening of rice spikelets, and formed a jasmonic acid phytohormone, corona toxin, linolenic acid, 12-oxo-phytodienoic acid, yamic acid and methyl yamate as active ingredients regulate the flowering time of parental parents in rice male sterile line breeding and hybrid seed production. Beneficial effect

Using the technology described in the present invention, the rice sterile line (or restorer line) can be made to start flowering within 10-120 minutes after implementing the technology, and an obvious flowering peak will appear. When the time difference is selected and the specific implementation time is selected, the flowering peaks of the sterile line and the maintainer line or the restorer line can be well met, thereby improving the yield of rice sterile line breeding or hybrid seed production. Compared with the existing flowering time regulation technology, the present invention has the advantage that: on the day (within 10-120 minutes) after the technology is implemented, the sterile line or the restorer line can be made to bloom, forming a flowering peak period, and With maintainer line (such as male sterile line breeding), restorer line (such as: "three-line method" seed production, or indica rice as male parent "two-line method" seed production) or sterile line (such as: japonica rice as male parent The flowering peaks of the "two-line method" seed production) of the subspecies meet.

4. Description of Drawings Figure 1 Chemical Structural Formula

5. Specific implementation

Example 1: In September 1998, this technology was implemented in the Aiyou 15 combination (Ai A (japonica three-line male sterile line)×D15) farming field of Wuxian City Agricultural Science Institute, Suzhou City, Jiangsu Province.

Spray 0.2 mg, 1.5 mg, 10 mg, 30 mg, and 50 mg of methyl jasmonate/square meter on the dwarf A CMS line, and the CMS line starts to bloom 100, 80, 60, 40, and 30 minutes after treatment , the flowering peak formed meets the flowering peak of the restorer line D15. The seed setting rate of the control was 5.2%, and the seed setting rates of the treatments were 10.1%, 12.5%, 15.8%, 20.1%, and 24.1%, respectively.

Spray 0.2 mg, 1.5 mg, 10 mg, 30 mg, and 50 mg of jasmonic acid/square meter respectively to the dwarf A male sterile lines, and the male sterile lines begin to bloom at 110, 95, 72, 45, and 33 minutes after treatment, forming The flowering peak of the restorer line D15 met. The seed setting rate of the control was 5.2%, and the seed setting rates of the treatments were 13.1%, 15.1%, 15.8%, 20.1%, and 22.1%, respectively.

Spray 0.2 mg, 1.5 mg, 10 mg, 30 mg, and 50 mg of yamsic acid/square meter respectively to the dwarf A CMS, and the CMS begins to flower at 115, 100, 75, 60, and 53 minutes after treatment respectively. The formed flowering peak coincides with the flowering peak of the restorer line D15. The seed setting rate of the control was 5.2%, and the seed setting rates of the treatments were 10.1%, 10.1%, 12.8%, 14.3%, and 18.1%, respectively.

Embodiment 2: In September 1998, this technology was implemented in the dwarf A (japonica type three-line sterile line) sterile line breeding field of Wuxian City Agricultural Science Institute, Suzhou City, Jiangsu Province.

Spray 1.5 milligrams, 10 milligrams, 30 milligrams, 60 milligrams of methyl jasmonate/square meter respectively to the dwarf A male sterile line, and the male sterile line begins to bloom at 80, 60, 40, and 28 minutes respectively, and the flowering peak period formed is the same as The flowering peaks of the maintainer lines meet.

Spray 1.5 mg, 10 mg, 30 mg, and 60 mg of jasmonic acid/square meter to the dwarf A CMS line, and the CMS line starts to bloom at 95, 72, 45, and 30 minutes respectively, and the flowering peak period formed is the same as that of the maintainer line The flowering peaks meet.

Embodiment 3: In August 1998, this technology was implemented in Xinlu A (indica type three-line CMS) and Xiang 125s (indica type two-line CMS) test field at the experimental station of the Department of Agronomy of Jiangxi Agricultural University in August 1998.

Spray 1.5 mg, 10 mg, and 30 mg methyl jasmonate/square meter on the Xinlu A CMS line respectively, and the CMS line starts to bloom 60, 40, and 28 minutes after treatment, and forms a peak flowering period.

Spraying 1.5 mg, 10 mg, and 30 mg of methyl jasmonate/square meter to the Xiang 125s indica-type two-line CMS respectively, the CMS began to bloom 72, 45, and 34 minutes after treatment, and formed a peak flowering period .

Example 4: In August 1998, this technology was implemented in two-line legal farming of Peiai 64s5JR1068 (indica and japonica subspecies hybrid combination) in Jiangxi Academy of Agricultural Sciences.

Spray 0.5 mg, 1 mg, 10 mg, 30 mg, and 600 mg of methyl jasmonate/square meter to the restorer line JR1068 (japonica type), respectively, and the sterile lines were treated at 100, 80, 60, 40, and 28 minutes respectively. Flowering begins, and the flowering peak formed meets the flowering peak of the sterile line Pei'ai 64s.

Spray 0.5 mg, 1 mg, 10 mg, 30 mg, and 600 mg of linolenic acid/m2 on the restorer line JR1068 (japonica type), and the sterile lines began to flower at 112, 97, 66, 54, and 48 minutes after treatment. , the formed flowering peak meets the flowering peak of the male sterile line Pei'ai 64s.

Example 5: In September 1998, this technology was implemented in the CMS dwarf A test field of Nanjing Agricultural University.

Spray 0.5 mg, 10 mg, and 40 mg of methyl potato acid per square meter to the male sterile line Dwarf A, and the male sterile lines began to flower at 70, 50, and 38 minutes after treatment, and formed a peak flowering period.

Spray 0.5 mg, 10 mg, and 40 mg of 12-oxophytodienoic acid/square meter on the dwarf A of the sterile line, and the sterile line begins to bloom 70, 45, and 30 minutes after the treatment, and forms a flowering peak .

Most of the flowering periods of the sterile lines mentioned in the document are familiar and mastered by those of ordinary skill in the art during the implementation process.

Claims (2)

1. a method for regulating and controlling flowering time for rice hybrid production, characterized in that: In the breeding of rice sterile lines, at the heading and flowering stage of sterile lines, jasmonic acid plant hormones, coronatine (Coronatine), linolenic acid, 12-oxophytodienoic acid, yamic acid, and methyl yamate As a regulator of the active ingredient, the sterile line is sprayed at a dose of 0.15-60.0 mg active ingredient/square meter, so that the flowering peak of the sterile line can be compared with the flowering peak of the maintainer line or with the flowering peak after 10-120 minutes. The restorer lines meet at the flowering peak; Or in the heading and flowering stage of the restorer line, use jasmonic acid plant hormones, coronatine (Coronatine), linolenic acid, 12-oxophytodienoic acid, yamic acid, and methyl yamate as regulators of active ingredients, according to The dose of 0.15-60.0 mg active ingredient/square meter is sprayed on the restorer line, so that the flowering peak of the restorer line can meet the flowering peak of the sterile line after 10-120 minutes. 2. The method for regulating flowering time for rice hybrid production according to claim 1, characterized in that: rice sterile lines refer to three-line sterile lines and two-line sterile lines.