CN113005633A - Antibacterial nanofiber membrane and preparation method and application thereof - Google Patents
Antibacterial nanofiber membrane and preparation method and application thereof Download PDFInfo
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- 239000002121 nanofiber Substances 0.000 title claims abstract description 106
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- 238000002360 preparation method Methods 0.000 title claims abstract description 21
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 32
- BERDEBHAJNAUOM-UHFFFAOYSA-N copper(I) oxide Inorganic materials [Cu]O[Cu] BERDEBHAJNAUOM-UHFFFAOYSA-N 0.000 claims abstract description 29
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- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 17
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- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 15
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- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims abstract description 13
- 238000009987 spinning Methods 0.000 claims description 24
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- 238000001035 drying Methods 0.000 claims description 12
- 238000001291 vacuum drying Methods 0.000 claims description 12
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- 238000005406 washing Methods 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 230000000845 anti-microbial effect Effects 0.000 claims description 4
- 239000004599 antimicrobial Substances 0.000 claims description 3
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 abstract description 6
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 6
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- 238000011065 in-situ storage Methods 0.000 abstract description 6
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- 239000003638 chemical reducing agent Substances 0.000 abstract description 4
- 238000005265 energy consumption Methods 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 239000002086 nanomaterial Substances 0.000 abstract description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 7
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- 238000012360 testing method Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003592 biomimetic effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
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- 229910001431 copper ion Inorganic materials 0.000 description 1
- PRNVEHKPCAVECT-UHFFFAOYSA-M copper(1+) sulfanide Chemical class [SH-].[Cu+] PRNVEHKPCAVECT-UHFFFAOYSA-M 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
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- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M11/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising
- D06M11/32—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with oxygen, ozone, ozonides, oxides, hydroxides or percompounds; Salts derived from anions with an amphoteric element-oxygen bond
- D06M11/36—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with oxygen, ozone, ozonides, oxides, hydroxides or percompounds; Salts derived from anions with an amphoteric element-oxygen bond with oxides, hydroxides or mixed oxides; with salts derived from anions with an amphoteric element-oxygen bond
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Abstract
The invention provides an antibacterial nanofiber membrane and a preparation method and application thereof, and belongs to the technical field of nanomaterials. According to the preparation method of the antibacterial nano-fiber membrane, the chitosan nano-fiber membrane is prepared by adopting an electrostatic spinning method, vitamin C beneficial to a human body is used as a reducing agent, and the nano-cuprous oxide antibacterial agent is prepared on the chitosan nano-fiber membrane by adopting an in-situ synthesis method, so that the chitosan nano-fiber membrane contains nano-cuprous oxide, and the antibacterial effectiveness and the broad spectrum of the chitosan nano-fiber membrane are improved; the adopted chitosan resource is rich, copper sulfate, sodium hydroxide and vitamin C are all common substances, the raw materials are low in price, the preparation process does not need high-temperature treatment, and the chitosan preparation method is simple and convenient and has the technical effects of low cost, high efficiency, no pollution and low energy consumption; the antibacterial nanofiber membrane has a structure of bionic natural extracellular matrix, nano cuprous oxide can be uniformly dispersed on the chitosan nanofiber membrane, and the antibacterial function is good.
Description
The present application is a divisional application of chinese patent application filed on 5.2.2018.2, CN201810111002.6, entitled "an antibacterial nanofiber membrane and method for making same", the entire contents of which are incorporated herein by reference.
Technical Field
The invention relates to the technical field of nano materials, in particular to an antibacterial nanofiber membrane and a preparation method and application thereof.
Background
Chitosan is the second largest polysaccharide next to cellulose on earth, is also the only basic amino acid polysaccharide chitosan found in nature at present, has good biocompatibility, biodegradability and antibacterial property, and is widely used in the biomedical fields of medical dressings, hemostatic articles, anti-adhesion membranes, tissue engineering scaffold materials and the like.
When the chitosan is used for medical dressing, the prepared chitosan nano fiber has larger specific surface area and high porosity, so the chitosan nano fiber has better oxygen permeation and air permeability, is beneficial to the respiration of wound tissue cells and the growth and regeneration of epithelial tissues, and can obviously accelerate the healing of wounds. Although chitosan has a certain antibacterial property and has a high fatality rate only to fungi, it does not have a broad antibacterial spectrum. Therefore, in order to increase the antibacterial performance of chitosan nanofibers, the prior art generally loads a nano silver antibacterial agent on chitosan nanofibers, for example, chinese patent CN101297976A discloses a preparation method of an antibacterial silver/chitosan nanofiber membrane, and patent CN104511045A discloses a dressing containing nano silver polyvinyl alcohol/chitosan nanofiber membrane and preparation thereof. Patent CN101187111A discloses a gelatin/chitosan composite nanofiber felt containing nano silver for medical dressing and preparation thereof. However, in these patents, nano silver is loaded on chitosan nanofiber membrane, so as to improve the antibacterial performance of chitosan nanofiber membrane, and although the antibacterial performance of nano silver is good, the price is high, the cost is difficult to control, and the nano silver is easy to discolor, which is not beneficial to production and popularization.
Disclosure of Invention
The invention aims to provide an antibacterial nanofiber membrane and a preparation method and application thereof, aiming at the problems in the prior art.
In order to achieve the above object, the present invention provides the following technical solutions:
in a first aspect of embodiments of the present invention, there is provided a method for preparing an antibacterial nanofiber membrane, the method including:
dissolving purified chitosan in a mixed solvent of 3% acetic acid and dimethyl sulfoxide, dissolving for 12h at 30 ℃ by using a magnetic stirrer to obtain a spinning solution with the concentration of 3.0%, and then performing an electrostatic spinning process on the spinning solution to obtain a chitosan nanofiber membrane with the average fiber diameter of 188 +/-62 nm, wherein the process parameters of the electrostatic spinning process comprise: the spinning voltage is 16kV, the receiving distance is 150mm, and the spinning speed is 0.6 mL/h;
crosslinking the chitosan nanofiber membrane by glutaraldehyde steam for 12h, then putting the chitosan nanofiber membrane into a drying chamber for vacuum drying, and then putting the chitosan nanofiber membrane into a culture dish;
sequentially adding 5mL of copper sulfate solution, 2.5mL of sodium hydroxide solution and 5-10 mL of vitamin C solution into the culture dish in which the chitosan nanofiber membrane is placed, and standing at a preset temperature for a preset time to obtain the chitosan nanofiber membrane containing nano cuprous oxide;
and taking out the chitosan nano fibrous membrane containing the nano cuprous oxide from the culture dish, washing with deionized water, and putting into a drying chamber for vacuum drying to obtain the antibacterial nano fibrous membrane.
In a preferred embodiment, the solvents of the copper sulfate solution, the sodium hydroxide solution and the vitamin C solution are all deionized water, and the concentration of each solution is 0.02mol/L, 0.2mol/L and 0.04mol/L respectively.
In a preferred embodiment, the molar ratio of copper sulfate, sodium hydroxide and vitamin C in the culture dish is 1:5: 1.5-3.
In a preferred embodiment, the antibacterial nanofiber membrane has a specification of 3cm × 3 cm.
In a preferred embodiment, the preset time period is 6-24 h.
In a preferred embodiment, the predetermined temperature is 20-25 ℃.
According to a second aspect of the embodiments of the present invention, there is provided an antibacterial nanofiber membrane prepared by any one of the above methods for preparing an antibacterial nanofiber membrane, wherein the antibacterial nanofiber membrane contains cuprous oxide nanoparticles and chitosan.
The invention has the following positive technical effects:
according to the antibacterial nano fibrous membrane and the preparation method thereof provided by the invention, the chitosan nano fibrous membrane is prepared by adopting an electrostatic spinning method from the structure of a bionic extracellular matrix, vitamin C beneficial to a human body is used as a reducing agent, and the nano cuprous oxide antibacterial agent is prepared on the chitosan nano fibrous membrane by adopting an in-situ synthesis method, so that the chitosan nano fibrous membrane contains nano cuprous oxide, and the antibacterial effectiveness and the broad spectrum property of the chitosan nano fibrous membrane are improved; in addition, the chitosan resource adopted by the invention for preparing the antibacterial nanofiber membrane is rich, copper sulfate, sodium hydroxide and vitamin C are all common substances, the price of raw materials is low, the preparation process does not need high-temperature treatment, the preparation process is simple and convenient, and the technical effects of low cost, high efficiency, no pollution and low energy consumption are achieved; finally, the antibacterial nanofiber membrane provided by the invention has a structure of a bionic natural extracellular matrix, and the nano cuprous oxide synthesized by the in-situ synthesis method can be uniformly dispersed on the chitosan nanofiber membrane, so that two substances with antibacterial properties are organically combined together, a better antibacterial function is given to the antibacterial nanofiber membrane, and the application field of the antibacterial nanofiber membrane is wider.
Drawings
Fig. 1 is a method flow diagram illustrating a method of making an antimicrobial nanofiber membrane according to one exemplary embodiment.
Fig. 2 is a scanning electron microscope image of an antibacterial nanofiber provided by the invention.
Detailed Description
The present invention is described in detail below with reference to specific embodiments (but not limited to) and the accompanying drawings, the specific method of the embodiments is only for illustrating the invention, the scope of the invention is not limited by the embodiments, the invention can be applied to various modifications and changes of shape and structure, and these equivalents based on the invention are also within the scope of the claims of the present invention.
Fig. 1 is a process flow diagram illustrating a method of making an antimicrobial nanofiber membrane according to one exemplary embodiment, the method comprising:
step 101, dissolving purified chitosan in a mixed solvent of 3% acetic acid and dimethyl sulfoxide, dissolving for 12 hours at 30 ℃ by using a magnetic stirrer to obtain a spinning solution with the concentration of 3.0%, and then performing an electrostatic spinning process on the spinning solution to obtain a chitosan nanofiber membrane with the average fiber diameter of 188 +/-62 nm, wherein the process parameters of the electrostatic spinning process comprise: the spinning voltage was 16kV, the take-up distance was 150mm, and the spinning rate was 0.6 mL/h.
102, crosslinking the chitosan nanofiber membrane by glutaraldehyde steam for 12 hours, then putting the chitosan nanofiber membrane into a drying chamber for vacuum drying, and then putting the chitosan nanofiber membrane into a culture dish.
The chitosan nanofiber membrane after crosslinking is insoluble in water.
And 103, sequentially adding 5mL of copper sulfate solution, 2.5mL of sodium hydroxide solution and 5-10 mL of vitamin C solution into the culture dish on which the chitosan nanofiber membrane is placed, and standing at a preset temperature for a preset time to obtain the chitosan nanofiber membrane containing nano cuprous oxide.
In the chitosan nanofiber membrane containing cuprous oxide nanoparticles, cuprous oxide nanoparticles can be uniformly dispersed in the chitosan nanofiber membrane.
And 104, taking out the chitosan nano fibrous membrane containing the nano cuprous oxide from the culture dish, washing with deionized water, and putting into a drying chamber for vacuum drying to obtain the antibacterial nano fibrous membrane.
In a preferred embodiment, the solvents of the copper sulfate solution, the sodium hydroxide solution and the vitamin C solution are all deionized water, and the concentration of each solution is 0.02mol/L, 0.2mol/L and 0.04mol/L respectively.
In a preferred embodiment, the molar ratio of copper sulfate, sodium hydroxide and vitamin C in the culture dish is 1:5: 1.5-3.
In a preferred embodiment, the antibacterial nanofiber membrane is 3cm × 3cm in size.
In a preferred embodiment, the preset time period is 6-24 h.
In a preferred embodiment, the predetermined temperature is 20-25 ℃.
The nano cuprous oxide is a novel antibacterial agent, is low in cost and non-toxic relative to silver ions, has an antibacterial effect, and is derived from the fact that the nano cuprous oxide can react with SH-, -S-S bonds in microorganisms to generate corresponding sulfhydryl copper compounds to disturb the physiological reaction of the microorganisms to cause the death of the microorganisms.
The chitosan antibacterial nanofiber membrane containing the nano cuprous oxide is prepared by using amino complexing copper ions on chitosan molecules, using vitamin C beneficial to human bodies as a reducing agent and adopting an in-situ synthesis method, and the whole preparation process is simple in method, low in cost and environment-friendly.
As shown in fig. 2, fig. 2 is a scanning electron microscope image of an antibacterial nanofiber provided by the present invention, and as is apparent from fig. 2, the antibacterial nanofiber membrane prepared by the present invention has a structure of a biomimetic natural extracellular matrix, and has a large specific surface area, a high porosity, and good air permeability and moisture permeability, and the nano cuprous oxide is uniformly dispersed on the chitosan nanofiber, which is more favorable for inhibiting the growth of bacteria and healing of wounds.
To better illustrate the technical effects of the antibacterial nanofiber membrane prepared by the preparation method of the antibacterial nanofiber membrane provided by the invention, examples 1-3 are specifically shown.
Example 1
(1) Dissolving purified chitosan in a mixed solvent of 3% acetic acid and dimethyl sulfoxide, dissolving for 12h at 30 ℃ by using a magnetic stirrer to obtain a spinning solution with the concentration of 3.0%, and then performing an electrostatic spinning process on the spinning solution to obtain a chitosan nanofiber membrane with the average fiber diameter of 188 +/-62 nm, wherein the process parameters of the electrostatic spinning process comprise: the spinning voltage was 16kV, the take-up distance was 150mm, and the spinning rate was 0.6 mL/h.
(2) And (3) crosslinking the chitosan nanofiber membrane by glutaraldehyde steam for 12h, putting the chitosan nanofiber membrane into a drying chamber for vacuum drying, and then putting the chitosan nanofiber membrane into a culture dish.
(3) And sequentially adding 5mL of 0.02mol/L copper sulfate solution, 2.5mL of 0.2mol/L sodium hydroxide solution and 5mL of 0.04mol/L vitamin C solution into the culture dish on which the chitosan nanofiber membrane is placed, and standing at 20 ℃ for 6 hours to obtain the chitosan nanofiber membrane containing cuprous oxide nanoparticles.
(4) And taking out the chitosan nano fibrous membrane containing the nano cuprous oxide from the culture dish, washing with deionized water, and putting into a drying chamber for vacuum drying to obtain the antibacterial nano fibrous membrane.
Example 2
(1) Dissolving purified chitosan in a mixed solvent of 3% acetic acid and dimethyl sulfoxide, dissolving for 12h at 30 ℃ by using a magnetic stirrer to obtain a spinning solution with the concentration of 3.0%, and then performing an electrostatic spinning process on the spinning solution to obtain a chitosan nanofiber membrane with the average fiber diameter of 188 +/-62 nm, wherein the process parameters of the electrostatic spinning process comprise: the spinning voltage was 16kV, the take-up distance was 150mm, and the spinning rate was 0.6 mL/h.
(2) And (3) crosslinking the chitosan nanofiber membrane by glutaraldehyde steam for 12h, putting the chitosan nanofiber membrane into a drying chamber for vacuum drying, and then putting the chitosan nanofiber membrane into a culture dish.
(3) And sequentially adding 5mL of 0.02mol/L copper sulfate solution, 2.5mL of 0.2mol/L sodium hydroxide solution and 5mL of 0.04mol/L vitamin C solution into the culture dish on which the chitosan nanofiber membrane is placed, and standing at 20 ℃ for 12 hours to obtain the chitosan nanofiber membrane containing cuprous oxide nanoparticles.
(4) And taking out the chitosan nano fibrous membrane containing the nano cuprous oxide from the culture dish, washing with deionized water, and putting into a drying chamber for vacuum drying to obtain the antibacterial nano fibrous membrane.
Example 3
(1) Dissolving purified chitosan in a mixed solvent of 3% acetic acid and dimethyl sulfoxide, dissolving for 12h at 30 ℃ by using a magnetic stirrer to obtain a spinning solution with the concentration of 3.0%, and then performing an electrostatic spinning process on the spinning solution to obtain a chitosan nanofiber membrane with the average fiber diameter of 188 +/-62 nm, wherein the process parameters of the electrostatic spinning process comprise: the spinning voltage was 16kV, the take-up distance was 150mm, and the spinning rate was 0.6 mL/h.
(2) And (3) crosslinking the chitosan nanofiber membrane by glutaraldehyde steam for 12h, putting the chitosan nanofiber membrane into a drying chamber for vacuum drying, and then putting the chitosan nanofiber membrane into a culture dish.
(3) And sequentially adding 5mL of 0.02mol/L copper sulfate solution, 2.5mL of 0.2mol/L sodium hydroxide solution and 10mL of 0.04mol/L vitamin C solution into the culture dish on which the chitosan nanofiber membrane is placed, and standing at 20 ℃ for 12 hours to obtain the chitosan nanofiber membrane containing cuprous oxide nanoparticles.
(4) And taking out the chitosan nano fibrous membrane containing the nano cuprous oxide from the culture dish, washing with deionized water, and putting into a drying chamber for vacuum drying to obtain the antibacterial nano fibrous membrane.
The hygienic standards of GB 15979-: x ═ (a-B)/a × 100%, where: x is the bacteriostasis rate (%), A is the average colony number of the sample before oscillation, and B is the average colony number of the sample after oscillation. Evaluation criteria: the number of colonies without addition of the sample group was 1 x 104-9*104Between cfu/mL, andthe difference of the average colony number before and after sample oscillation is within 10 percent, and the test is effective.
Before the antibacterial performance test, the chitosan nanofiber and the antibacterial nanofiber membrane are treated by 75% ethanol steam, and the specifications of the chitosan nanofiber and the antibacterial nanofiber membrane are both 3cm multiplied by 3 cm.
Table 1 antibacterial property test results of antibacterial nanofiber membrane
Table 1 the results show that: in terms of antibacterial performance, the antibacterial effect of the antibacterial nanofiber membrane prepared in the embodiments 1 to 3 on escherichia coli is obviously superior to that of the common chitosan nanofiber membrane, and the antibacterial effect on staphylococcus aureus is also obviously superior to that of the common chitosan nanofiber membrane, so that the antibacterial nanofiber membrane prepared by the preparation method of the antibacterial nanofiber membrane provided by the invention has a more excellent antibacterial effect.
In conclusion, the antibacterial nanofiber membrane and the preparation method thereof provided by the invention start from the structure of the bionic extracellular matrix, the chitosan nanofiber membrane is prepared by adopting an electrostatic spinning method, vitamin C beneficial to a human body is used as a reducing agent, and the nano cuprous oxide antibacterial agent is prepared on the chitosan nanofiber membrane by adopting an in-situ synthesis method, so that the chitosan nanofiber membrane contains nano cuprous oxide, and the antibacterial effectiveness and the broad spectrum of the chitosan nanofiber membrane are improved; in addition, the chitosan resource adopted by the invention for preparing the antibacterial nanofiber membrane is rich, copper sulfate, sodium hydroxide and vitamin C are all common substances, the price of raw materials is low, the preparation process does not need high-temperature treatment, the preparation process is simple and convenient, and the technical effects of low cost, high efficiency, no pollution and low energy consumption are achieved; finally, the antibacterial nanofiber membrane provided by the invention has a structure of a bionic natural extracellular matrix, and the nano cuprous oxide can be uniformly dispersed on the chitosan nanofiber membrane by an in-situ synthesis method, so that two substances with antibacterial properties are organically combined together, a better antibacterial function is given to the antibacterial nanofiber membrane, and the application field of the antibacterial nanofiber membrane is wider.
While the invention has been described in detail in the foregoing by way of general description, and specific embodiments and experiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. This invention is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. It will be understood that the invention is not limited to the precise arrangements described above and shown in the drawings and that various modifications and changes may be made without departing from the scope thereof.
Claims (4)
1. A method of preparing an antimicrobial nanofiber membrane, comprising:
dissolving purified chitosan in a mixed solvent of 3% acetic acid and dimethyl sulfoxide, dissolving for 12h at 30 ℃ by using a magnetic stirrer to obtain a spinning solution with the concentration of 3.0%, and then performing an electrostatic spinning process on the spinning solution to obtain a chitosan nanofiber membrane with the average fiber diameter of 188 +/-62 nm, wherein the process parameters of the electrostatic spinning process comprise: the spinning voltage is 16kV, the receiving distance is 150mm, and the spinning speed is 0.6 mL/h;
crosslinking the chitosan nanofiber membrane by glutaraldehyde steam for 12h, then putting the chitosan nanofiber membrane into a drying chamber for vacuum drying, and then putting the chitosan nanofiber membrane into a culture dish;
sequentially adding 5mL of 0.02mol/L copper sulfate solution, 2.5mL of 0.2mol/L sodium hydroxide solution and 10mL of 0.04mol/L vitamin C solution into the culture dish on which the chitosan nanofiber membrane is placed, and standing at 20 ℃ for 12 hours to obtain the chitosan nanofiber membrane containing cuprous oxide nanoparticles;
and taking out the chitosan nano fibrous membrane containing the nano cuprous oxide from the culture dish, washing with deionized water, and putting into a drying chamber for vacuum drying to obtain the antibacterial nano fibrous membrane.
2. The method of claim 1, wherein the antimicrobial nanofiber membrane has a gauge of 3cm x 3 cm.
3. An antibacterial nanofiber membrane, which is prepared by the preparation method of the antibacterial nanofiber membrane of claim 1 or 2, wherein the antibacterial nanofiber membrane contains cuprous oxide nanoparticles and chitosan.
4. Use of the antibacterial nanofibrous membrane of claim 3 in the antibacterial field, characterized in that before use, the antibacterial nanofibrous membrane is steamed with ethanol.
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| CN115387116B (en) * | 2022-09-02 | 2023-08-22 | 浙江技立新材料股份有限公司 | A kind of biological antibacterial dyed nanofiber film and preparation method thereof |
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