CN113366120A - 纳米孔测序方法 - Google Patents

纳米孔测序方法 Download PDF

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CN113366120A
CN113366120A CN201880099752.4A CN201880099752A CN113366120A CN 113366120 A CN113366120 A CN 113366120A CN 201880099752 A CN201880099752 A CN 201880099752A CN 113366120 A CN113366120 A CN 113366120A
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nanopore
nucleic acid
stranded
nanopore sequencing
loop
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CN113366120B (zh
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朱国丽
沈寒婕
林建勋
江媛
曾涛
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Hangzhou Huada Xufeng Technology Co ltd
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BGI Shenzhen Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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Abstract

一种纳米孔测序方法，包括：在纳米孔测序装置中，以核酸单链环或双链环为模板进行滚环扩增，产生出扩增的单链，在电场的作用下，将扩增的单链引入纳米孔测序装置的纳米孔中，使扩增的单链通过纳米孔并产生每个碱基的电信号，通过判断不同碱基引起的电信号差异，实现对模板核酸上碱基序列的测定。

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PCT国内申请，说明书已公开。

Claims (20)

  1. PCT国内申请，权利要求书已公开。
CN201880099752.4A 2018-12-07 2018-12-07 纳米孔测序方法 Active CN113366120B (zh)

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PCT/CN2018/119911 WO2020113581A1 (zh) 2018-12-07 2018-12-07 纳米孔测序方法

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CN113366120B CN113366120B (zh) 2024-05-14

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114807331A (zh) * 2022-05-12 2022-07-29 中国海洋大学 一种短链dna的纳米孔测序方法
WO2023122883A1 (zh) * 2021-12-27 2023-07-06 深圳华大生命科学研究院 成膜装置及成膜方法
WO2024124567A1 (zh) 2022-12-16 2024-06-20 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
WO2025138260A1 (zh) * 2023-12-29 2025-07-03 深圳华大生命科学研究院 多肽和蛋白质的测序方法、试剂盒及应用

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* Cited by examiner, † Cited by third party
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CN113736850A (zh) * 2021-08-13 2021-12-03 纳昂达(南京)生物科技有限公司 基于双链环化的文库构建方法及其在测序中的应用
CN114774411B (zh) * 2022-06-16 2022-10-21 序康医疗科技（苏州）有限公司 大片段dna环化连接方法
EP4613874A4 (en) * 2022-12-29 2025-12-31 Bgi Shenzhen Sequencing locator, sequencing locator complex, method for multiple target nucleic acid sequence amplifications, and nanopore sequencing method
CN120265789A (zh) * 2022-12-29 2025-07-04 深圳华大生命科学研究院 测序接头、测序接头复合物、靶核酸序列多次纳米孔测序的方法
CN117987521A (zh) * 2023-02-08 2024-05-07 深圳市真迈生物科技有限公司 测序方法
CN117987522A (zh) * 2023-02-08 2024-05-07 深圳市真迈生物科技有限公司 测序方法
WO2025222416A1 (zh) * 2024-04-24 2025-10-30 深圳华大生命科学研究院 测序文库的构建方法、核酸测序方法、纳米孔测序方法及应用

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WO2000018957A1 (en) * 1998-09-30 2000-04-06 Applied Research Systems Ars Holding N.V. Methods of nucleic acid amplification and sequencing
US20100331194A1 (en) * 2009-04-10 2010-12-30 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
CN103733063A (zh) * 2011-05-27 2014-04-16 牛津纳米孔技术有限公司 偶联方法
CN107002130A (zh) * 2014-11-11 2017-08-01 深圳华大基因研究院 多程测序
WO2018114706A1 (en) * 2016-12-20 2018-06-28 F. Hoffmann-La Roche Ag Single stranded circular dna libraries for circular consensus sequencing

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US20020197618A1 (en) * 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
CA2577079C (en) * 2004-08-13 2014-05-20 President And Fellows Of Harvard College An ultra high-throughput opti-nanopore dna readout platform
EP2652153B1 (en) * 2010-12-17 2017-07-05 The Trustees of Columbia University in the City of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
JP6333179B2 (ja) 2012-01-20 2018-05-30 ジニア テクノロジーズ， インコーポレイテッド ナノポアベースの分子検出および配列決定
US9650670B2 (en) * 2012-10-28 2017-05-16 The Regents Of The University Of California Capacitive feedback (transimpedance) amplifier
CN104406952B (zh) * 2014-11-19 2017-03-29 上海纳米技术及应用国家工程研究中心有限公司 基于滚环扩增技术和核壳纳米金结构的sers基底制备方法
CN114703264B (zh) * 2015-06-03 2024-09-20 亿明达股份有限公司 使用锚定至邻近纳米孔的聚合酶的系链对多核苷酸测序的组合物、系统和方法
CN107727705B (zh) * 2017-09-28 2019-12-10 东南大学 一种酶反应检测纳米孔电学传感器

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000018957A1 (en) * 1998-09-30 2000-04-06 Applied Research Systems Ars Holding N.V. Methods of nucleic acid amplification and sequencing
US20100331194A1 (en) * 2009-04-10 2010-12-30 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
CN103733063A (zh) * 2011-05-27 2014-04-16 牛津纳米孔技术有限公司 偶联方法
CN107002130A (zh) * 2014-11-11 2017-08-01 深圳华大基因研究院 多程测序
WO2018114706A1 (en) * 2016-12-20 2018-06-28 F. Hoffmann-La Roche Ag Single stranded circular dna libraries for circular consensus sequencing

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023122883A1 (zh) * 2021-12-27 2023-07-06 深圳华大生命科学研究院 成膜装置及成膜方法
CN114807331A (zh) * 2022-05-12 2022-07-29 中国海洋大学 一种短链dna的纳米孔测序方法
WO2024124567A1 (zh) 2022-12-16 2024-06-20 深圳华大生命科学研究院 纳米孔测序方法和试剂盒
EP4636094A1 (en) 2022-12-16 2025-10-22 BGI Shenzhen Nanopore sequencing method and kit
WO2025138260A1 (zh) * 2023-12-29 2025-07-03 深圳华大生命科学研究院 多肽和蛋白质的测序方法、试剂盒及应用

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US12398421B2 (en) 2025-08-26
CN113366120B (zh) 2024-05-14
US20220002798A1 (en) 2022-01-06

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