CN113782102B - DNA data storage method, device, equipment and readable storage medium - Google Patents

Abstract

The application relates to the technical field of data storage, and provides a method, a device and equipment for storing DNA data and the field of readable storage media. The method comprises the following steps: acquiring a base sequence corresponding to a binary sequence of target data; dividing the base sequence to obtain S sequence units, wherein each sequence unit comprises a plurality of divided sequence fragments, the S sequence units contain K sequence fragments in total, and the length of each sequence fragment is n; labeling the K sequence fragments and the S sequence units by using preset index information to obtain the K labeled sequence fragments and the S labeled sequence units, wherein the index information comprises a first retrieval sequence for expressing the arrangement sequence of the S sequence units in the base sequence and a second retrieval sequence for expressing the arrangement sequence of a plurality of sequence fragments belonging to the same sequence unit in the sequence units. The method provided by the application can realize the storage of large-scale data information in DNA.

Description

DNA data storage method, device, equipment and readable storage medium

technical field

The present application belongs to the technical field of data storage, and in particular relates to a DNA data storage method, device, equipment and readable storage medium.

Background technique

The development of the era of artificial intelligence and big data has higher and higher demand for data storage, and there is an urgent need for new storage media with high storage density, long storage time, and low maintenance costs. Deoxyribonucleic acid (DeoxyriboNucleic Acid, DNA), as an information storage medium developed in recent years, is considered to be one of the most potential mediums for future information storage.

DNA molecules have four bases, which are: adenine (A), cytosine (Cytosine, C), guanine (Guanine, G) and thymine (Thymine, T). The DNA-based data storage technology uses the above four base sequences to represent the data series composed of binary "0" and "1". Compared with traditional storage media, DNA data storage has the characteristics of high storage density, long storage time, low maintenance cost, and good biocompatibility. For example: 1g of DNA can store more than one million high-definition movies, and its data storage density is more than 7 orders of magnitude higher than the current silicon-based storage media such as traditional hard disks; at the same time, DNA can store data stably for more than a thousand years, which is a hundred times the storage time of existing storage media above. In addition, the maintenance cost of DNA is low, and the maintenance cost of storing for a hundred years is only one ten-thousandth of the current existing media.

The DNA data storage process usually includes the following steps: (1) Extract binary information from computer information such as pictures, videos, and texts; (2) According to the preset correspondence between binary and bases A, T, C, and G, the Binary sequence information is converted into A/T/C/G sequences (that is, DNA sequences) formed by base A, T, C, and G codes and stored with data information; (3) DNA synthesis technology or other technologies are used to convert the coded The A/T/C/G sequence is converted into a DNA chemical polymer molecule and stored in a suitable environment. Afterwards, when it is necessary to obtain the stored data, the following steps can be performed: (4) Utilize DNA sequencing technology to interpret the stored DNA chemical polymer molecules into A/T/C/G sequences; (5) Utilize appropriate The decoding method converts A/T/C/G sequences into binary information; (6) converts binary information into computer information such as pictures, videos, and texts.

Among them, the data encoding problem is the core problem in the current DNA data storage methods.

Contents of the invention

One of the purposes of the embodiments of the present application is to provide a DNA data storage method, device, device and readable storage medium, aiming at solving the data encoding problem in the DNA data storage technology.

The technical scheme that the embodiment of the present application adopts is:

In the first aspect, a method for storing DNA data is provided, including:

Obtain the base sequence corresponding to the binary sequence of the target data;

Segmenting the base sequence to obtain S sequence units, and each sequence unit includes a plurality of segmented sequence fragments, wherein, the S sequence units contain K sequence fragments in total, and the sequence fragments The length of is n, and n, S and K are all integers greater than or equal to 2;

The K sequence fragments and the S sequence units are marked by using the preset index information to obtain K mark sequence fragments and S mark sequence units, wherein the index information includes information used to represent the S sequence units A first search sequence for the arrangement order of sequence units in the base sequence, and a second search sequence for indicating the arrangement order of multiple sequence fragments belonging to the same sequence unit in the sequence unit , K pieces of the marker sequence fragments are used to synthesize K first DNA molecules storing the target data.

In one embodiment, using the second retrieval sequence to mark multiple sequence fragments belonging to the same sequence unit includes:

splicing a second search sequence on either side of said sequence fragment, or

The search base groups are spliced simultaneously on both sides of the sequence fragment, and the search base groups on both sides form the second search sequence.

In one embodiment, the first search sequence includes i DNA sequence fragments, i is an integer greater than or equal to 1, and each of the DNA sequence fragments includes the first base sequence used as an index mark and the Mark the second base sequence of the sequence unit number.

In one embodiment, the DNA sequence fragments corresponding to the first search sequence and the second search sequence are obtained using DNA synthesis technology. Exemplarily, DNA synthesis techniques include, but are not limited to, enzymatic synthesis, phosphoramidite synthesis, and the like.

In one embodiment, the DNA sequence fragments corresponding to the first search sequence and the second search sequence can be amplified from a pre-synthesized DNA universal molecular library, such as PCR technology.

In one embodiment, the storage method also includes:

After synthesizing K first DNA molecules storing the target data from the K marker sequence fragments, storing the K first DNA molecules in S first physical spaces, wherein the same sequences belong to one The first DNA molecules corresponding to the marker sequence fragments of the unit are stored in the same first physical space, and the first DNA molecules corresponding to the marker sequence fragments that do not belong to the same sequence unit are stored in Different from said first physical space.

In one embodiment, the s first physical spaces are integrated into one DNA hard disk.

In one embodiment, the storage method also includes:

storing a second DNA molecule in a second physical space corresponding to the first physical space, the second DNA molecule storing the index information.

In one embodiment, the decoding method for the K first DNA molecules includes:

Sequencing the plurality of first DNA molecules stored in each of the first physical spaces to obtain a plurality of marker sequence fragments; pairing each marker sequence unit belonging to the same marker sequence unit according to the second retrieval sequence splicing the sequence fragments corresponding to the marker sequence fragments to obtain the sequence unit;

splicing the obtained S sequence units according to the first search sequence to obtain the base sequence;

The base sequence is converted into the target data.

In a second aspect, a DNA data storage device is provided, including a data processing module,

The data processing module is used to obtain the base sequence corresponding to the binary sequence of the target data; segment the base sequence to obtain K sequence fragments with a length of n, and the K sequence fragments are divided into S Sequence units, S and K are both integers greater than or equal to 2; use preset index information to mark K said sequence fragments and S said sequence units to obtain K marked sequence fragments and S marked sequence units , wherein, the index information includes a first search sequence used to represent the arrangement order of the S sequence units in the base sequence, and a first search sequence used to represent multiple sequences belonging to the same sequence unit The second retrieval sequence of the arrangement order of the fragments in the sequence unit, the K fragments of the marker sequence are used to synthesize the K first DNA molecules storing the target data.

In one embodiment, the device further includes: a DNA synthesis module, configured to synthesize K first DNA molecules storing the target data from the K marker sequence fragments.

In one embodiment, the device further includes: a DNA molecule storage module, configured to store K first DNA molecules in S first physical spaces, wherein the markers belonging to one sequence unit The first DNA molecules corresponding to the sequence fragments are stored in the same first physical space, and the first DNA molecules corresponding to the marker sequence fragments that do not belong to the same sequence unit are stored in different first physical spaces. a physical space.

In one embodiment, the DNA molecule storage module is also used to store the second DNA molecule in the second physical space.

In one embodiment, it also includes a DNA molecule sequencing module, configured to sequence a plurality of first DNA molecules stored in each of the first physical spaces to obtain a plurality of marker sequence fragments; the data The processing module is further configured to splice the sequence fragments corresponding to each of the tag sequence fragments belonging to the same tag sequence unit according to the second retrieval sequence, to obtain the sequence unit;

splicing the obtained S sequence units according to the first search sequence to obtain the base sequence;

The base sequence is converted into the target data.

In a third aspect, a DNA data storage device is provided, including a terminal device, the terminal device includes a memory, a processor, and a computer program stored in the memory and operable on the processor, characterized in That is, when the processor executes the computer program, the method for storing DNA data as described in the first aspect is realized.

In a fourth aspect, a computer-readable storage medium is provided. The computer-readable storage medium stores a computer program. When the computer program is executed by a processor, the method for storing DNA data in the first aspect is implemented.

In a fifth aspect, a DNA hard disk is provided, including a plurality of physical spaces, the physical spaces are made of physical materials, and each of the physical spaces is used to store DNA molecules.

In one embodiment, the physical material is at least one of SiO ₂ , metal oxides, and polymer materials. These physical materials form a physical space that wraps DNA molecules, and at the same time, isolates different DNA molecules.

The beneficial effect of the DNA data storage method, device, device and readable storage medium provided by the embodiment of the present application is that: the present application divides the base sequence corresponding to the binary sequence of the target data into S sequences, and each sequence unit includes A plurality of segmented sequence fragments, the S sequence units contain K sequence fragments in total, and the length of the sequence fragments is n, n, S and K are all integers greater than or equal to 2, using the preset The index information marks the position information of sequence fragments and sequence units, and the marked sequence fragments are synthesized into DNA molecules and stored separately. Through this method, the storage capacity of data information can be increased, and the storage of large-scale data information in DNA can be realized.

In one implementation situation, the length of the first retrieval sequence is different from that of the marker sequence fragment (the sequence fragment with the second retrieval sequence), and the first retrieval sequence and the marker sequence fragment are distinguished from the sequence units by length difference. Specifically, m represents the base number of the marker sequence fragment, q represents the base number of the second retrieval sequence, i represents the number of DNA sequence fragments in the first retrieval sequence, and p represents the number of DNA sequence fragments in the first retrieval sequence The number of bases in the two-base sequence, through the method provided by this application, can realize the storage of DNA data containing D bases, wherein, the calculation formula of D is as follows:

D ^＝ 4q×(mq)× ^4i×p

In a specific embodiment, when the length of m is 8 bases, q=4, i=10, p=4, D=256×4×4 ⁴⁰ =1.23×10 ²⁷ . When 1 base stores 2 bits of information, the amount of information that can be stored is L=2.46×10 ²⁷ bits=3.075×10 ²⁶ bytes=3.075×10 ⁵ ZB, which is much larger than the current data storage scale.

In another implementation situation, the length of the first search sequence is the same as the length of the marker sequence fragment (sequence fragment with the second search sequence); the first base sequence used as an index mark in the first search sequence can be It is a part of the second search base sequence, and the base number of the first base sequence is the same as that of the second search base sequence. In this case, let m represent the base number of the marker sequence fragment, q represent the base number of the second search sequence, use i to represent the number of DNA sequence fragments in the first search sequence, and p represent the first search sequence The number of bases in the second base sequence in the sequence can be stored by the method provided by this application, and the DNA data containing D bases can be stored, wherein, the calculation formula of D is as follows:

D＝( ^4q- i)×(mq)× ^4i×p

In a specific embodiment, when the length of m is 8 bases, q=4, i=10, p=4, D=(256-10)×4×4 ⁴⁰ =1.18×10 ²⁷ . When 1 base stores 2 bits of information, the amount of information that can be stored is L=2.36×10 ²⁷ bits=2.95×10 ²⁶ bytes=2.95×10 ⁵ ZB, which is much larger than the current data storage scale.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the accompanying drawings that need to be used in the embodiments or exemplary technical descriptions. Obviously, the accompanying drawings in the following descriptions are only for this application. For some embodiments, those skilled in the art can also obtain other drawings based on these drawings without creative efforts.

Figure 1 is a schematic diagram of the composition of the DNA storage device provided by the embodiment of the present application;

Fig. 2 is the process flow diagram of the storage and writing of DNA data provided by the embodiment of the present application;

Fig. 3 is a schematic diagram of a sequence unit comprising multiple sequence fragments formed after the base sequence in S101 provided in the embodiment of the present application is segmented;

Fig. 4 is the sequence unit in S103 provided by the embodiment of the present application after the first search sequence mark, and each sequence fragment in the sequence unit has been marked by the second search sequence, to obtain information sequence units respectively containing multiple information sequence fragments schematic diagram;

Fig. 5 is a schematic diagram of DNA storage after K first DNA molecules are stored in S different first physical spaces provided by the embodiment of the present application;

Fig. 6 is the flow chart of the interpretation process of the DNA data provided by the embodiment of the present application;

FIG. 7 is a schematic structural diagram of a terminal device provided by an embodiment of the present application.

detailed description

In order to make the purpose, technical solution and advantages of the present application clearer, the present application will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present application.

In the following description, specific details such as specific system structures and technologies are presented for the purpose of illustration rather than limitation, so as to thoroughly understand the embodiments of the present application. It will be apparent, however, to one skilled in the art that the present application may be practiced in other embodiments without these specific details. In other instances, detailed descriptions of well-known systems, devices, circuits, and methods are omitted so as not to obscure the description of the present application with unnecessary detail.

It should be understood that the term "and/or" used in the description of the present application and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations. In addition, in the description of the specification and appended claims of the present application, the terms "first", "second", "third" and so on are only used to distinguish descriptions, and should not be understood as indicating or implying relative importance.

It should also be understood that references to "one embodiment" or "some embodiments" or the like described in the specification of the present application mean that a particular feature, structure or characteristic described in connection with the embodiment is included in one or more embodiments of the present application . Thus, appearances of the phrases "in one embodiment," "in some embodiments," "in other embodiments," "in other embodiments," etc. in various places in this specification are not necessarily All refer to the same embodiment, but mean "one or more but not all embodiments" unless specifically stated otherwise. The terms "including", "comprising", "having" and variations thereof mean "including but not limited to", unless specifically stated otherwise.

At present, data encoding is the core technical issue in DNA data storage technology, especially for large-scale data, there is a lack of effective data encoding methods to achieve large-scale data storage. In view of this, the present application provides a method for storing DNA data. In the process of data encoding, the method first converts the binary sequence of the data to be stored into the corresponding base sequence, and then divides the base sequence twice. The base sequence is divided into S sequence units, and each sequence unit includes a plurality of segmented sequence fragments, wherein the S sequence units contain K total of the sequence fragments. Then use the preset index information to mark the K sequence fragments and the S sequence units. Finally, the K marker sequence fragments are synthesized into DNA molecules and then stored. Based on the data encoding method provided by this application, DNA data storage can be effectively realized.

Furthermore, the data encoding method provided by the present application has obvious advantages in terms of data storage capacity.

The storage method is realized by the DNA data storage device shown in FIG. 1 . The DNA data storage device includes a data processing module, a DNA molecule synthesis module, a DNA molecule storage module, and a DNA molecule sequencing module.

Wherein, the data processing module is used to implement data encoding and decoding. For example, the data to be stored is converted into binary information, and the binary information is converted into a base sequence according to the preset correspondence between binary data and bases. Afterwards, the base sequence is encoded according to the preset index information to obtain the base sequence that is finally used to generate the DNA molecule.

The DNA molecule synthesis module is used to synthesize DNA molecules according to the encoded base sequence. The DNA molecule storage module can store DNA molecules. The DNA molecular sequencing module is used to translate DNA molecules into base sequences. Correspondingly, the data processing module can also decode the base sequence obtained by sequencing in the DNA molecule according to the index information, and obtain the data stored in the DNA molecule through data conversion.

In the embodiment of the present application, the DNA data storage device may be a complete DNA data storage device, that is, a device integrated with multiple functional modules. The device can realize the complete process of data encoding, DNA molecule synthesis, DNA molecule storage, DNA molecule sequencing and data decoding.

In another embodiment, the DNA data storage device may also be a system composed of individual devices.

For example, the data processing module may be computer equipment such as computers, servers, and robots. Used to implement data encoding and decoding. The DNA molecular synthesis module can be a DNA molecular synthesizer, which is used to synthesize DNA molecules according to the coded base sequence. The DNA molecule storage module may be a DNA hard disk capable of storing DNA molecules. The DNA molecular sequencing module may be a DNA molecular sequencer, capable of realizing the function of DNA molecular sequencing.

Fig. 2 is a schematic diagram of the implementation flow of a DNA data storage method provided by the embodiment of the present application, specifically including:

S101. Obtain the base sequence corresponding to the binary sequence of the data to be stored.

In this step, obtaining the base sequence corresponding to the binary sequence of the data to be stored refers to converting the binary sequence of the data to be stored into a base sequence formed by A, T, C, and G codes and storing data information .

In some embodiments, obtaining the base sequence corresponding to the binary sequence of the data to be stored includes:

S111. Extract the binary sequence corresponding to the data to be stored.

The data to be stored is any data information that may exist in the terminal device, and may include text, pictures, sound, video, software, programs and other information, but is not limited thereto.

When extracting the binary sequence corresponding to the data to be stored, the coding information corresponding to the data to be stored may be obtained, and the corresponding coding information is converted into binary coding information, thereby obtaining the corresponding binary sequence. For example, the text in the text information can be converted into the corresponding ASCII (English full name is American Standard Code for Information Interchange, Chinese full name is American Standard Code for Information Interchange) encoding, UNICODE (English full name is Universal Character Set, Chinese full name is Universal Character Set) ) encoding, and then convert the encoded information into a binary sequence.

示例性的，提取文本“春，已不再是想象之外的那只蝴蝶。”对应的二进制序列为“11100110 10011000 10100101 11101111 10111100 10001100 11100101 1011011110110010 11100100 10111000 10001101 11100101 10000110 10001101 1110011010011000 10101111 11100110 10000011 10110011 11101000 10110001 1010000111100100 10111001 10001011 11100101 10100100110110 11100111 10011011010010010010010010000010 10100011 11100101 100011111010101010100100100100100100100100100110100110110110110110110110110110110110110110110110110110110 1110110 1110110 11100100011110 1110111 100100011110 111110 111100 111100 111100 111100 that

S121. Convert the binary sequence into a base sequence according to a preset mapping rule.

In the embodiment of the present application, the preset mapping rule refers to the preset mapping rule between binary and base. According to the preset mapping rules between the binary code and the bases A, T, C, G, the binary sequence consisting of 0/1 is converted into a code formed by the bases A, T, C, G, and stored with data information base sequence. Exemplarily, the preset correspondence between the binary sequence and bases A, T, C, and G is as follows: a base A represents a 00, a base T represents a 01, a base C represents a 10, and a base C represents a 10. The base G represents an 11. When the binary sequence is 00110110101100101011011011000011001001, according to the preset mapping rules between binary and bases A, T, C, G, the binary data information is converted into a base sequence into a DNA sequence of AGTCCGACCGTCGAAGACT. Of course, the preset correspondence between the binary code and the bases A, T, C, and G is not limited to the above example, for example, it can also be: a base T represents a 00, a base A represents a 01, and a base The base G represents a 10, and the base C represents an 11, but not limited thereto. It should be understood that the preset mapping rules between binary sequences and bases A, T, C, and G only need to be able to convert binary sequences into base sequences according to the preset mapping rules, and are not limited to the above examples.

Exemplarily, the mapping rule between binary and base is: a base A represents a 11, a base T represents a 10, a base C represents a 01, and a base G represents a 00.根据该映射规则，可以将文本“春，已不再是想象之外的那只蝴蝶。”对应的二进制序列“11100110 10011000 1010010111101111 10111100 10001100 11100101 10110111 10110010 11100100 1011100010001101 11100101 10000110 10001101 11100110 10011000 10101111 1110011010000011 10110011 11101000 10110001 10100001 11100100 10111001 1000101111100101 10100100 10010110 11100111 10011010 10000100 11101001 1000001010100011 11100101 10001111 10101010 11101000 10011101 10110100 1110100010011101 10110110 11100011 10000000 10000010”转换为碱基序列“ATCT TCTG TTCCATAA TAAG TGAG ATCC TACA TAGT ATCG TATG TGAC ATCC TGCT TGAC ATCT TCTG TTAAATCT TGGA TAGA ATTG TAGC TTGC ATCG TATC TGTA ATCC TTCG TCCT ATCA TCTT TGCGATTC TGGT TTGA ATCC TGAA TTTT ATTG TCAC TACG ATTG TCAC TACT ATGA TGGG TGGT".

S102. Segment the base sequence to obtain S sequence units, and each sequence unit includes a plurality of segmented sequence fragments, wherein, the S sequence units contain K sequence fragments in total, and the length of the sequence fragments is n, n , S and K are all integers greater than or equal to 2.

In the embodiment of the present application, the base sequence is segmented, and the segmented base sequence becomes s sequence units, and each sequence unit includes a plurality of segmented sequence fragments, thereby reducing the sequence length and facilitating subsequent steps to Sequence units are stored separately. It should be understood that the length referred to in the embodiments of the present application refers to the base length, which can be understood as the number of bases.

Each sequence unit obtained after base sequence segmentation includes multiple sequence fragments with a length of n, therefore, the length of the sequence unit is an integer multiple of the sequence fragments. In some embodiments, the sequence units formed after segmentation have the same length, that is, the base sequence is divided into s sequence units of the same length, and the s sequence units of the same length contain the same number of sequences of length n fragment. In some embodiments, the lengths of the sequence units formed after segmentation are different. Exemplarily, the base sequence is sequentially segmented according to the preset sequence unit length to obtain S-1 sequence units, and the remaining The length of the base sequence is less than the preset length. At this time, the remaining base sequence is regarded as a sequence unit, and its length is less than the length of other sequence units. In some embodiments, the base sequence can be segmented according to other preset segmentation rules to obtain s sequence units with different sequence unit lengths.

In a possible implementation manner, the base sequence is segmented to obtain s sequence units, and each sequence unit includes a plurality of segmented sequence fragments, including:

Divide the base sequence into multiple sequence units, and the length of the sequence unit is an integer multiple of n;

Divide each sequence unit into multiple sequence fragments of length n.

In some embodiments, when the base sequence is divided into multiple sequence units, starting from one end of the base sequence, the base sequence is divided every other preset sequence unit length to obtain s sequence units, wherein , the default length is an integer multiple of n. When the length of the remaining base sequence after the last division is less than the preset length, the remaining base sequence is regarded as a sequence unit. In other embodiments, the base sequence can also be segmented starting from other positions in the base sequence. For example, starting from the middle position of the base sequence, the base sequence is sequentially divided towards both ends at the same time, to obtain sequence units whose length is an integer multiple of n.

In some embodiments, each sequence unit is divided into a plurality of sequence fragments of length n, including:

Starting from one end of the sequence unit, the sequence unit is divided every length n to obtain multiple sequence fragments. In other embodiments, the sequence unit can also be segmented starting from other positions of the sequence unit. For example, starting from the middle position of the sequence unit, the sequence unit is sequentially segmented towards both ends at the same time to obtain a sequence fragment with a length of 1.

Exemplarily, as shown in Figure 3, in the base sequence in S101, according to the standard that the length of the sequence unit is 60 bases, the base sequence is sequentially segmented from one end of the base sequence, and three groups of lengths are obtained. A sequence unit of 60 bases, and a group of sequence units with a length of 12 bases; according to the standard of a sequence fragment length of 4 bases, the sequence units are sequentially divided from one end of the sequence unit, and the length of the three groups is The sequence unit of 60 bases was divided into 15 groups of sequence fragments, and the sequence unit of 12 bases was divided into 3 groups of sequence fragments.

In another possible implementation, the base sequence is segmented to obtain s sequence units, and each sequence unit includes multiple sequence fragments with a length of n, including:

Divide the base sequence into multiple sequence fragments of length n;

The sequence fragments are combined according to a preset combination rule to obtain s sequence units.

In some embodiments, the base sequence is divided into multiple sequence fragments with a length of n, starting from one end of the base sequence, the base sequence is divided every length n to obtain multiple sequence fragments.

In some embodiments, when the sequence fragments are combined according to the preset combination rules, the preset combination rules refer to the rules for assigning multiple sequence fragments as a sequence unit, including the sequence fragments assigned to a sequence unit in the base The position in the base sequence, the number of sequence fragments and the arrangement order of the sequence fragments when the sequence fragments are combined into a sequence unit. The lengths of the combined sequence units can be the same or different, but they are all integer multiples of 1. Exemplarily, according to the order of the sequence fragments in the base sequence, 20 sequence fragments with a number of 6 bases are sequentially combined to form a sequence unit.

Exemplarily, the length of the sequence segment is 4, and the sequence unit includes 15 sequence segments. At this time, the base sequence of step S101 "ATCT TCTG TTCC ATAA TAAG TGAG ATCC TACA TAGT ATCG TATG TGAC ATCC TGCTTGAC ATCT TCTG TTAA ATCT TGGA TAGA ATTG TAGC TTGC ATCG TATC TGTA ATCC TTCGTCCT ATCA TCTT TGCG ATTC TGGT TTGACTTG ATCC TGAA TT TTC A ATTG TCACTACT ATGA TGGG TGGT” to obtain 48 sequence fragments, which are: ATCT, TCTG, TTCC, ATAA, TAAG, TGAG, ATCC, TACA, TAGT, ATCG, TATG, TGAC, ATCC, TGCT, TGAC, ATCT, TCTG, TTAA, ATCT, TGGA, TAGA, ATTG, TAGC, TTGC, ATCG, TATC, TGTA, ATCC, TTCG, TCCT, ATCA, TCTT, TGCG, ATTC, TGGT, TTGA, ATCC, TGAA, TTTT, ATTG, TCAC, TACG, ATTG, TCAC, TACT, ATGA, TGGG, TGGT; according to the preset combination rules and the length of the sequence unit, each sequence fragment is combined in turn, and every 15 sequence fragments are combined into a sequence unit to obtain four sequences The unit contains the following sequence fragments respectively. The first sequence unit includes the following 15 sequence fragments: ATCT, TCTG, TTCC, ATAA, TAAG, TGAG, ATCC, TACA, TAGT, ATCG, TATG, TGAC, ATCC, TGCT, TGAC; the second sequence unit includes the following 15 sequence fragments: ATCT, TCTG, TTAA, ATCT, TGGA, TAGA, ATTG, TAGC, TTGC, ATCG, TATC, TGTA, ATCC, TTCG, TCCT; the third sequence unit includes the following 15 sequence fragments: ATCA, TCTT, TGCG, ATTC, TGGT, TTGA, ATCC, TGAA, TTTT, ATTG, TCAC, TACG, ATTG, TCAC, TACT; the fourth sequence unit includes the following three sequence fragments: ATGA, TGGG, TGGT.

In the embodiment of the present application, after the base sequence is segmented, S sequence units are obtained, and the S sequence units contain K sequence fragments with a length of n in total.

S103. Use the preset index information to mark the K sequence fragments and S sequence units to obtain K sequence fragments and S sequence units, wherein the index information includes information used to indicate that the S sequence units are in the base The first search sequence of the arrangement order in the base sequence, and the second search sequence used to indicate the arrangement order of multiple sequence fragments belonging to the same sequence unit in the sequence unit, K marker sequence fragments are used to synthesize and store the target The K first DNA molecules of the data.

In the embodiment of the present application, the preset index information is used to mark the sequence unit, so as to facilitate the decoding of subsequent DNA storage data. Using the preset index information to mark the sequence units includes: using the first search sequence to mark the arrangement order of the S sequence units in the base sequence, and using the second search sequence to mark the sequence units belonging to the same sequence unit The order in which the multiple sequence fragments are arranged in the sequence unit is marked to obtain K marked sequence fragments and S marked sequence units. In this case, the sequence of S sequence units in the base sequence is recorded, and at the same time, the sequence of multiple sequence fragments belonging to the same sequence unit is also recorded.

In the embodiment of the present application, the second search sequence is a sequence formed of bases, and the base sequence of the second search sequence can be determined according to preset rules. Exemplarily, when the number of sequence fragments in the sequence unit is less than or equal to 4, a single base can be used to represent the second search sequence. For example: the sequence number 1 corresponds to the double base A, the sequence number 2 corresponds to the double base C, the sequence number 3 corresponds to the double base G, and the sequence number 4 corresponds to the double base T. Of course, the correspondence between the sequence number and the base is not limited to this method. Exemplarily, when the number of sequence fragments in the sequence unit is less than or equal to 16, two bases may be used to represent the second search sequence. For example: sequence number 1 corresponds to the two bases AA, sequence number 2 corresponds to the two bases AC, sequence number 3 corresponds to the two bases AG, sequence number 4 corresponds to the two bases AT, sequence number 5 corresponds to the two bases CA, and sequence number 6 corresponds to the two bases CC. The sequence number 7 corresponds to the two-base CG, the sequence number 8 corresponds to the two-base CT, the sequence number 9 corresponds to the two-base GA, the sequence number 10 corresponds to the two-base GC, the sequence number 11 corresponds to the two-base GG, the sequence number 12 corresponds to the two-base GT, and the sequence number 13 Corresponding to the two-base TA, the sequence number 14 corresponds to the two-base TC, the sequence number 15 corresponds to the two-base TG, and the sequence number 16 corresponds to the two-base TT.... Of course, the number of bases in the reference base group is not limited to 2. When the number of sequence fragments in the sequence unit increases, the number of bases in the second search sequence used increases correspondingly, such as when the sequence fragments in the sequence unit When the number of is less than or equal to 64, three bases can be used to represent the second search sequence. According to the rule that the power of 4 bases in the second search sequence is greater than or equal to the number of sequence fragments in the sequence unit, and so on.

In the embodiment of the present application, when the second search sequence is used to mark the sequence fragment, the second search sequence can be spliced at a specific position of the sequence fragment. In some embodiments, using the second search sequence to mark multiple sequence fragments belonging to the same sequence unit includes: splicing the second search sequence on either side of the sequence fragment. Exemplarily, the second search sequence is spliced at the start end, that is, the left end of the sequence fragment; or, the second search sequence is spliced at the end end, that is, the right end of the sequence fragment. In one embodiment, the method of using the second search sequence to mark multiple sequence fragments belonging to the same sequence unit includes: simultaneously splicing base groups on both sides of the sequence fragment, and the base groups on both sides form the second search sequence .

In the embodiment of the present application, K sequence fragments are marked by the second search sequence to form K marked sequence fragments, which are also called information sequence fragments. Exemplarily, after the second search sequence AA is marked before the sequence fragment ATGC, a marked sequence fragment of AAATGC is formed.

In the embodiment of the present application, the position of the S sequence units in the base sequence is marked by using the first search sequence. In some embodiments, the first search sequence includes i DNA sequence fragments, where i is an integer greater than or equal to 1, that is, the first search sequence may be one DNA sequence fragment or multiple DNA sequence fragments. Wherein, each DNA sequence fragment includes a first base sequence used as an index mark and a second base sequence used to indicate the sequence unit number. Wherein, the first base sequence can be placed in a specific position of the first search sequence according to preset settings. Exemplarily, the first base sequence is located at the initial segment (left end) of the first search sequence; exemplary, the first The base sequence is located at the termination segment (right end) of the first search sequence; exemplary, the first base sequence is located at a specific position in the first search sequence, such as the third and fourth bases of the first search sequence, Not limited to this.

The first base sequence can be set in advance. Exemplarily, TT is placed as the first base sequence at the initial segment of the first search sequence, and is used as an index mark, indicating the first search sequence of the DNA sequence fragment starting with TT in the sequence unit. In this example, when the first base sequence is located at the initial segment of the first search sequence, the first base sequence is different from the initial base sequence of the second search sequence, so as to avoid misidentifying sequence fragments during the identification process is the first search sequence.

Similarly, the second base sequence can also be preset. Exemplarily, the sequence number 1 corresponds to the four-base AAAA, the sequence number 2 corresponds to the four-base AAAC, the sequence number 3 corresponds to the four-base AAAG, and the sequence number 4 corresponds to the four-base AAAT. Of course, the correspondence between the sequence number and the base is not limited to this , and the number of bases in the reference base set is not limited to four. In the embodiment of the present application, the sequence unit is marked by the first search sequence, and each sequence fragment in the sequence unit is marked by the second search sequence, and the marked sequence unit is obtained, which is also called the formation information sequence unit. Referring to Fig. 4, after the sequence unit in step S102 (shown on the left side of the arrow in Fig. 4) is marked with the first search sequence, and each sequence segment in the sequence unit is marked with the second search sequence, a sequence containing multiple information is obtained respectively. Four sets of marker sequence units for the fragment (shown to the right of the arrow in Figure 4).

In some embodiments, the DNA sequence fragments corresponding to the first search sequence and the second search sequence are obtained using DNA synthesis technology. Exemplarily, DNA synthesis techniques include, but are not limited to, enzymatic synthesis, phosphoramidite synthesis, and the like.

In some embodiments, the DNA sequence fragments corresponding to the first search sequence and the second search sequence can be amplified from a pre-synthesized DNA universal molecular library, such as PCR technology.

The above steps S101 to S103 are implemented by the processing module in the device shown in FIG. 1 .

In some embodiments, the storage method also includes:

S104. After synthesizing K first DNA molecules storing the target data with K marker sequence fragments, store the K first DNA molecules in S first physical spaces, wherein the marker sequences belonging to the same sequence unit First DNA molecules corresponding to the fragments are stored in the same first physical space, and first DNA molecules corresponding to marker sequence fragments that do not belong to the same sequence unit are stored in different first physical spaces.

In this step, the K marker sequence fragments are respectively synthesized into K first DNA molecules storing the target data, which is realized by the DNA synthesis module in the device shown in FIG. 1 . In the embodiment of the present application, the K marker sequence fragments can be synthesized respectively through the existing synthesis technology to obtain K first DNA molecules.

Store the K first DNA molecules in S different first physical spaces, and store the first DNA molecules corresponding to the marker sequence fragments belonging to one sequence unit in the same first physical space, not belonging to the same sequence unit The first DNA molecules corresponding to the marker sequence fragments are stored in different first physical spaces, and this step is realized by the DNA storage module in the device shown in FIG. 1 . A schematic diagram of DNA storage after storing K first DNA molecules in S different first physical spaces is shown in FIG. 5 , wherein each small square represents a first physical space.

In this step, the synthesized K first DNA molecules are respectively stored in different first physical spaces, so as to realize the separate storage of each information sequence unit. Further, the S first physical spaces are integrated into one DNA hard disk to realize the storage of the base sequence storing the target data. Through the integration, the K first DNA molecules in the S first physical spaces form a complete whole to be preserved, and it is not easy to omit and lose information during the preservation process, which is conducive to improving the integrity of data preservation. Correspondingly, in the process of decoding and releasing, by decoding and releasing the integrated first DNA molecule, the DNA base can be completely recovered and the integrity of the data can be maintained.

In some embodiments, the storage method further includes: storing the second DNA molecule in a second physical space corresponding to the first physical space, where index information is stored in the second DNA molecule. The second DNA molecule storing the index information is stored in a second physical space different from the first physical space to realize preservation of the index information.

In the DNA data storage method provided in the embodiment of the present application, after converting the binary sequence corresponding to the data to be stored into a base sequence, the base sequence corresponding to the binary sequence of the target data is divided into S sequences, and each sequence unit includes multiple segmented sequence fragments, the S sequence units contain K sequence fragments in total, and the length of the sequence fragments is n, n, S and K are all integers greater than or equal to 2, using a preset index The information marks the position information of sequence fragments and sequence units, and the marked sequence fragments are synthesized into DNA molecules and stored separately. Through this method, the storage capacity of data information can be increased, and the storage of large-scale data information in DNA can be realized.

In some specific embodiments, the length of the first retrieval sequence and the length of the marker sequence fragment (the sequence fragment with the second retrieval sequence) are different, and then the first retrieval sequence and the marker sequence fragment are distinguished from the sequence unit by length difference . Specifically, m represents the base number of the marker sequence fragment, q represents the base number of the second retrieval sequence, i represents the number of DNA sequence fragments in the first retrieval sequence, and p represents the number of DNA sequence fragments in the first retrieval sequence The number of bases in the two-base sequence, through the method provided by this application, can realize the storage of DNA data containing D bases, wherein, the calculation formula of D is as follows:

D ^＝ 4q×(mq)× ^4i×p

In a specific embodiment, when the length of m is 8 bases, q=4, i=10, p=4, D=256×4×4 ⁴⁰ =1.23×10 ²⁷ . When 1 base stores 2 bits of information, the amount of information that can be stored is L=2.46×10 ²⁷ bits=3.075×10 ²⁶ bytes=3.075×10 ⁵ ZB, which is much larger than the current data storage scale. In some specific embodiments, the length of the first search sequence is the same as the length of the marker sequence fragment (sequence fragment with the second search sequence); the first base sequence used as an index mark in the first search sequence can be It is a part of the second search sequence and has the same number of bases as the second search sequence. In this case, let m represent the base number of the marker sequence fragment, q represent the base number of the second search sequence, use i to represent the number of DNA sequence fragments in the first search sequence, and p represent the first search sequence The number of bases in the second base sequence in the sequence, through the method of this application, can realize the storage of DNA data containing D bases, wherein, the calculation formula of D is as follows:

D＝( ^4q- i)×(mq)× ^4i×p

In a specific embodiment, when the length of m is 8 bases, q=4, i=10, p=4, D=(256-10)×4×4 ⁴⁰ =1.18×10 ²⁷ . When 1 base stores 2 bits of information, the amount of information that can be stored is L=2.36×10 ²⁷ bits=2.95×10 ²⁶ bytes=2.95×10 ⁵ ZB, which is much larger than the current data storage scale.

Even if the sequence fragment contains 8 bases, it can realize data storage much larger than the current storage capacity. In addition, since each sequence unit and sequence fragment is marked with the search sequence, the method can successfully realize data recovery from sequence fragment to sequence unit, and from sequence unit to base sequence.

In addition, in the embodiment of the present application, the first DNA molecules are stored separately, and amplified and stored according to actual needs. In this case, after the first DNA molecule is included in the DNA molecule library, the backup information of the first DNA molecule can be extracted according to actual needs, thereby avoiding the trouble of re-synthesis each time and greatly reducing storage costs.

In one embodiment, as shown in Figure 6, the decoding method of the first K DNA molecules includes:

S201. Sequencing the multiple first DNA molecules stored in each first physical space to obtain multiple marker sequence fragments; splicing the sequence fragments corresponding to each marker sequence fragment belonging to the same marker sequence unit according to the index information, Get the sequence unit.

The method of sequencing the K first DNA molecules includes any method that can read DNA products, such as second-generation sequencing, third-generation sequencing, etc., to obtain multiple information sequence units to be decoded.

In one embodiment, K first DNA molecules are sequenced to obtain K marker sequence fragments respectively. In some embodiments, when the second DNA molecule is stored in the second physical space corresponding to the first physical space, and the second DNA molecule stores index information, the sequencing further includes: sequencing the second DNA molecule, obtaining the A retrieval sequence and index information of a second retrieval sequence.

The above step S201 can be implemented by the DNA sequencing module in the storage device shown in FIG. 1 .

S202. Splicing the sequence fragments corresponding to each marker sequence fragment belonging to the same marker sequence unit according to the second retrieval sequence to obtain S sequence units; splicing the obtained S sequence units according to the first retrieval sequence to obtain bases sequence.

In this step, the K retrieval fragments belonging to the S sequence units are spliced into base sequences by retrieval information. In the embodiment of the present application, the position information of the marker sequence unit in the base sequence is obtained according to the first search sequence; the position information or numbering information of multiple marker sequence fragments belonging to the same sequence unit is obtained according to the second search sequence. According to the obtained position information or numbering information of the multiple sequence fragments, the multiple sequence fragments are spliced into a sequence unit; according to the obtained position information or numbering information of the sequence units, S sequence units are spliced into a base sequence.

In some embodiments, according to the second search sequence, the sequence fragments corresponding to each marker sequence fragment belonging to the same marker sequence unit are spliced to obtain S sequence units. The sequence fragment corresponding to each marker sequence fragment, and the position of the sequence fragment in the sequence unit; according to the position of the sequence fragment in the sequence unit, the sequence fragments are spliced into a sequence unit.

In one embodiment, the obtained S sequence units are spliced according to the first search sequence to obtain a base sequence, including:

Obtaining the position of the sequence unit in the base sequence according to the first search sequence;

According to the positions of the sequence units in the base sequence, the s sequence units are spliced into a base sequence.

Exemplarily, according to the first search sequence and the second search sequence, read out the complete DNA sequence "ATCT TCTGTTCC ATAA TAAG TGAG ATCC TACA TAGT ATCG TATG TGAC ATCC TGCT TGAC ATCT TCTGTTAA ATCT TGGA TAGA ATTG TAGC TTGC ATCG TATC TGTA ATCC TTCG TCCT ATCA TCTTTGCG ATTC TGGT TTGA ATCC TGAA TTTT ATTG TCAC TACG ATTG TCAC TACT ATGA TGGGTGGT".

S203. Convert the base sequence into target data.

The base sequence can be converted into a binary sequence by a preset mapping relationship that matches the data written in S101.比如，按照A对应11，T对应10，C对应01，G对应00，可以将S102得到的碱基序列转换为二进制序列为：“11100110 10011000 10100101 11101111 10111100 10001100 1110010110110111 10110010 11100100 10111000 10001101 11100101 10000110 1000110111100110 10011000 10101111 11100110 10000011 10110011 11101000 1011000110100001 11100100 10111001 10001011 11100101 10100100 10010110 1110011110011010 10000100 11101001 10000010 10100011 11100101 10001111 1010101011101000 10011101 10110100 11101000 10011101 10110110 11100011 1000000010000010”。

A sequence of computer information is then generated from the binary sequence.

According to the generated binary sequence, combined with the preset encoding rules, the binary sequence can be converted into corresponding data files, including files such as pictures, text, programs, audio, and video.

For example, a computer program is used to recover the binary sequence obtained in the above step S203 into the text information of "Chun, no longer the unimaginable butterfly."

It should be understood that the implementation of each step in the foregoing embodiments may be implemented by human calculation or by computer programs. Moreover, the sequence numbers of the above steps do not mean the order of execution, and the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation to the implementation process of the embodiment of the present application.

In the second aspect, with reference to FIG. 1 , the embodiment of the present application provides a DNA data storage device, including a data processing module, and a data processing module for obtaining the base sequence corresponding to the binary sequence of the target data; Segmentation to obtain S sequence units, and each sequence unit includes multiple segmented sequence segments, wherein, S sequence units contain K sequence segments in total, and the length of the sequence segment is n, and n, S and K are all greater than Or an integer equal to 2; use the preset index information to mark K sequence fragments and S sequence units to obtain K mark sequence fragments and S mark sequence units, wherein the index information includes the information used to represent the S sequence units The first search sequence for the arrangement order of the sequence unit in the base sequence, and the second search sequence for indicating the arrangement order of multiple sequence fragments belonging to the same sequence unit in the sequence unit, and the K marker sequence fragments are used for K first DNA molecules storing target data are synthesized.

In some embodiments, the data processing module is further configured to splice the sequence segments corresponding to each tag sequence segment belonging to the same tag sequence unit according to the second search sequence to obtain the sequence unit; the obtained S according to the first search sequence The sequence units are spliced to obtain the base sequence; the base sequence is converted into the target data.

In some embodiments, the storage device further includes a DNA synthesis module for synthesizing the K marker sequence fragments into K first DNA molecules storing target data. In some embodiments, the storage device further includes a DNA molecule storage module, configured to store K first DNA molecules in S first physical spaces, wherein the first DNA molecules corresponding to the marker sequence fragments belonging to one sequence unit Stored in the same first physical space, the first DNA molecules corresponding to marker sequence fragments that do not belong to the same sequence unit are stored in different first physical spaces.

In some embodiments, the DNA molecule storage module is also used to store a second DNA molecule in a second physical space.

In some embodiments, the storage device further includes a DNA molecule sequencing module, which performs sequencing on a plurality of first DNA molecules stored in each first physical space to obtain a plurality of marker sequence fragments;

The device containing the above modules in FIG. 1 can use DNA for data storage and writing, which corresponds to the method for DNA data storage and writing shown in FIG. 2 .

The embodiment of the present application also provides a DNA data storage device. As shown in FIG. 7 , the terminal device 70 provided in this embodiment includes: a processor 710 , a memory 720 , and a computer program 721 stored in the memory 720 and operable on the processor 710 . When the processor 710 executes the computer program 721 , the steps in the above-mentioned embodiments of the method for storing DNA data are implemented, such as steps S101 to S103 shown in FIG. 2 .

Exemplarily, the computer program 721 can be divided into one or more modules/units, and one or more modules/units are stored in the memory 720 and executed by the processor 710 to complete the present application. One or more modules/units may be a series of computer program instruction segments capable of accomplishing specific functions, and the instruction segments may be used to describe the execution process of the computer program 721 in the terminal device. For example, the computer program 721 may be divided into data processing modules. In some embodiments, the computer program 721 can also be divided into a data processing module, a DNA molecule synthesis module, a DNA molecule storage module and a DNA molecule storage module, and the specific functions of each module are as described in the text. In order to save space, details are not repeated here.

Those skilled in the art can understand that FIG. 7 is only an example of a terminal device 70, and does not constitute a limitation to the terminal device 70. It may include more or less components than those shown in the figure, or combine certain components, or be different. parts.

The processor 710 may be a central processing unit (Central Processing Unit, CPU), and may also be other general-purpose processors, digital signal processors (Digital Signal Processor, DSP), application specific integrated circuits (Application Specific Integrated Circuit, ASIC), off-the-shelf Field-Programmable Gate Array (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. A general-purpose processor may be a microprocessor, or the processor may be any conventional processor, or the like.

The storage 720 may be an internal storage unit of the terminal device 70 , such as a hard disk or memory of the terminal device 70 . The memory 720 can also be an external storage device of the terminal device 70, such as a plug-in hard disk equipped on the terminal device 70, a smart memory card (Smart Media Card, SMC), a secure digital (Secure Digital, SD) card, a flash memory card (Flash Card) and so on. Further, the memory 720 may also include both an internal storage unit of the terminal device 70 and an external storage device. The memory 720 is used to store a computer program 721 and other programs and data required by the terminal device 70 . The memory 720 can also be used to temporarily store data that has been output or will be output.

An embodiment of the present application also provides a computer-readable storage medium, where a computer program is stored in the computer-readable storage medium, and when the computer program is executed by a processor, the processing methods of the foregoing embodiments are implemented.

The embodiment of the present application also provides a computer program product, which, when the computer program product is run on the terminal device, enables the terminal device to execute the methods for storing DNA data in the foregoing embodiments.

An embodiment of the present application provides a DNA hard disk. Referring to FIG. 5 , it includes multiple physical spaces made of physical materials, and each physical space is used to store DNA molecules.

In the embodiment of the present application, the physical space made of physical materials wraps DNA molecules, and the DNA molecules are isolated by physical materials. Wherein, the shape of the physical space is not strictly limited, and may be circular, square, or any other shape.

In some embodiments, the DNA molecules stored in the physical space include the above-mentioned first DNA molecule, and in this case, the physical space is the first physical space. Correspondingly, the first DNA molecule includes the second search sequence and sequence fragments. Each first physical space also stores the above-mentioned second retrieval sequence.

In some embodiments, the DNA molecules stored in the physical space include the above-mentioned second DNA molecules, and in this case, the physical space is the second physical space.

In some embodiments, the physical material is at least one selected from SiO ₂ , metal oxides, and polymer materials. Wherein, the high molecular polymer material includes resin, but is not limited to resin.

The above are only optional embodiments of the application, and are not intended to limit the application. For those skilled in the art, various modifications and changes may occur in this application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present application shall be included within the scope of the claims of the present application.

Claims (14)

1. A method for storing DNA data, comprising:

   acquiring a base sequence corresponding to a binary sequence of target data;

   dividing the base sequence to obtain S sequence units, wherein each sequence unit comprises a plurality of divided sequence fragments, the S sequence units contain K sequence fragments, the length of each sequence fragment is n, and n, S and K are integers greater than or equal to 2;

   labeling the K sequence segments and the S sequence units by using preset index information to obtain K labeled sequence segments and S labeled sequence units, wherein the index information comprises a first retrieval sequence used for representing the arrangement sequence of the S sequence units in the base sequence and a second retrieval sequence used for representing the arrangement sequence of a plurality of sequence segments belonging to the same sequence unit in the sequence unit, and the K labeled sequence segments are used for synthesizing K first DNA molecules storing the target data;

   the storage method further comprises the following steps:

   after synthesizing K marked sequence segments into K first DNA molecules storing the target data, storing the K first DNA molecules in S first physical spaces, wherein the first DNA molecules corresponding to the marked sequence segments belonging to the same sequence unit are stored in the same first physical space, and the first DNA molecules corresponding to the marked sequence segments not belonging to the same sequence unit are stored in different first physical spaces.
2. 2. The method for storing DNA data according to claim 1, wherein the labeling of the plurality of sequence fragments belonging to the same sequence unit with the second search sequence comprises:

   splicing a second search sequence on either side of the sequence fragment, or

   And simultaneously splicing retrieval base groups on two sides of the sequence fragment, wherein the retrieval base groups on the two sides form the second retrieval sequence.
3. 3. The method for storing DNA data according to claim 1, wherein the first search sequence includes i DNA sequence fragments, i is an integer of 1 or more, and each of the DNA sequence fragments includes a first base sequence serving as an index marker and a second base sequence for identifying the sequence unit number.
4. 4. The method according to claim 1, wherein s first physical spaces are integrated in one DNA hard disk.
5. 5. The method for storing DNA data according to claim 1, further comprising:

   storing a second DNA molecule in a second physical space corresponding to the first physical space, the second DNA molecule storing the index information.
6. 6. The method for storing DNA data according to any one of claims 1 to 5, wherein the method for decoding K first DNA molecules comprises:

   sequencing a plurality of the first DNA molecules stored in each of the first physical spaces to obtain a plurality of the marker sequence fragments;

   splicing the sequence fragments corresponding to each of the labeled sequence fragments belonging to the same labeled sequence unit according to the second retrieval sequence to obtain the sequence unit; splicing the obtained S sequence units according to the first retrieval sequence to obtain the base sequence;

   converting the base sequence into the target data.
7. 7. A DNA data storage device is characterized by comprising a data processing module,

   the data processing module is used for acquiring a base sequence corresponding to the binary sequence of the target data; dividing the base sequence to obtain S sequence units, wherein each sequence unit comprises a plurality of divided sequence fragments, the S sequence units contain K sequence fragments in total, the length of each sequence fragment is n, and n, S and K are integers greater than or equal to 2; labeling the K sequence segments and the S sequence units by using preset index information to obtain K labeled sequence segments and S labeled sequence units, wherein the index information comprises a first retrieval sequence used for representing the arrangement sequence of the S sequence units in the base sequence and a second retrieval sequence used for representing the arrangement sequence of a plurality of sequence segments belonging to the same sequence unit in the sequence unit, and the K labeled sequence segments are used for synthesizing K first DNA molecules storing the target data;

   the device further comprises: a DNA molecule storage module,

   for storing K of said first DNA molecules in S first physical spaces, wherein said first DNA molecules corresponding to said marker sequence fragments belonging to one of said sequence units are stored in the same one of said first physical spaces and said first DNA molecules corresponding to said marker sequence fragments not belonging to the same one of said sequence units are stored in a different one of said first physical spaces.
8. 8. The DNA data storage device of claim 7, further comprising: and the DNA molecule synthesis module is used for synthesizing the K marking sequence segments into K first DNA molecules in which the target data are stored.
9. 9. The DNA data storage device of claim 7, wherein the DNA molecule storage module is further configured to store a second DNA molecule in a second physical space.
10. 10. The DNA data storage device of claim 7, further comprising a DNA molecule sequencing module for sequencing a plurality of the first DNA molecules stored in each of the first physical spaces to obtain a plurality of the marker sequence fragments;

    the data processing module is further configured to splice the sequence segments corresponding to each of the labeled sequence segments belonging to the same labeled sequence unit according to the second search sequence to obtain the sequence unit; splicing the obtained S sequence units according to the first retrieval sequence to obtain the base sequence; converting the base sequence into the target data.
11. 11. A DNA data storage device comprising a terminal device including a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor implements the storage method of DNA data according to any one of claims 1 to 6 when executing the computer program.
12. 12. A computer-readable storage medium storing a computer program, wherein the computer program, when executed by a processor, implements the method for storing DNA data according to any one of claims 1 to 6.
13. 13. A DNA hard disk, characterized by comprising a plurality of physical spaces made of physical materials, wherein each physical space is used for storing DNA molecules, and the DNA molecules are stored according to the storage method of any one of claims 1 to 6.
14. 14. The DNA hard disk of claim 13 characterized in that the physical material is selected from SiO ₂ At least one of metal oxide and polymer material.

Images