CN115339135B - A method for preparing silk fibroin colored contact lenses - Google Patents
A method for preparing silk fibroin colored contact lenses Download PDFInfo
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- CN115339135B CN115339135B CN202210949220.3A CN202210949220A CN115339135B CN 115339135 B CN115339135 B CN 115339135B CN 202210949220 A CN202210949220 A CN 202210949220A CN 115339135 B CN115339135 B CN 115339135B
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- silk fibroin
- lens
- solution
- acrylamide
- contact lens
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29D—PRODUCING PARTICULAR ARTICLES FROM PLASTICS OR FROM SUBSTANCES IN A PLASTIC STATE
- B29D11/00—Producing optical elements, e.g. lenses or prisms
- B29D11/00009—Production of simple or compound lenses
- B29D11/00038—Production of contact lenses
- B29D11/00048—Production of contact lenses composed of parts with dissimilar composition
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29D—PRODUCING PARTICULAR ARTICLES FROM PLASTICS OR FROM SUBSTANCES IN A PLASTIC STATE
- B29D11/00—Producing optical elements, e.g. lenses or prisms
- B29D11/00009—Production of simple or compound lenses
- B29D11/00038—Production of contact lenses
- B29D11/00076—Production of contact lenses enabling passage of fluids, e.g. oxygen, tears, between the area under the lens and the lens exterior
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Manufacturing & Machinery (AREA)
- Ophthalmology & Optometry (AREA)
- Mechanical Engineering (AREA)
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Abstract
The invention discloses a preparation method of a silk fibroin color contact lens, which comprises the steps of dissolving and dialyzing silk fibroin crude products, and reacting with acrylamide in a mold to prepare the silk fibroin contact lens; soaking and dyeing the lens by using the prepared anthocyanin aqueous solution after the lens is prepared; solves the technical problems of poor air permeability, hypoxia in cornea, redness, pain, dryness, even vision decline and the like of eyes caused by long-term wearing of the contact lens in the prior art.
Description
Technical Field
The invention belongs to the technical field of contact lenses, and particularly relates to a preparation method of a silk fibroin color contact lens.
Background
The contact lens is also called a cornea contact lens, is currently used for vision correction of patients with ametropia such as myopia, astigmatism and the like, and has the effects of treating and preventing eye diseases and the like. In addition to colorless transparent contact lenses, colored contact lenses incorporating pigment or dye molecules according to the process are also emerging on the market today. Most of the colored contact lenses adopt a sandwich technology, and the middle pigment layer is an iris-like pattern, so that the color contact lenses have the effects of brightening, blackening, enlarging and the like in decoration and beauty. Colored contact lenses are also widely sought after in the young due to their cosmetic effects. However, the present colored contact lenses are still mainly made of polymer hydrogel, silicon hydrogel and other materials, and the colored part is based on more metal-based pigments. The material in the colored contact lens can cause threat risk to the environment, and meanwhile, the potential health hazard to eyes is further increased. In addition, many contact lenses have poor breathability and can cause hypoxia in the cornea of the eye over time, causing redness, pain, dryness and even vision loss in the eye.
Disclosure of Invention
The invention aims to solve the technical problems that: the preparation method of the silk fibroin color contact lens is provided to solve the technical problems that the prior art contact lens has poor air permeability, and the cornea of the eye is lack of oxygen after being worn for a long time, so that the eye is red, swelling, pain, dryness, even vision decline and the like.
The technical scheme of the invention is as follows:
A silk fibroin color contact lens preparation method comprises dissolving silk fibroin crude product, dialyzing, and reacting with acrylamide in a mold to obtain silk fibroin contact lens; the lenses are soaked and dyed by the prepared anthocyanin aqueous solution after being prepared.
And uniformly mixing the acrylamide solution and the silk fibroin solution, then dripping the mixture into a contact lens mould, and air-drying to obtain the lens.
The concentration of the silk fibroin solution is 5% -15% (g/mL), and the concentration of the acrylamide solution is 25% -75% (g/mL); the acrylamide solution and the silk fibroin solution are uniformly mixed according to the volume ratio of 1:10.
The method for dissolving and dialyzing the crude silk fibroin product comprises the following steps: the silk fibroin crude product is put into sterile ultrapure water to be fully immersed, the sterile ultrapure water is dissolved by ultrasonic cleaner, the solution is dialyzed for 3-5 days in the sterile ultrapure water, and the sterile ultrapure water is replaced every 6-24 hours.
Cleaning and drying a contact lens mold by using sterile water, and then adding an acrylamide and silk fibroin mixed solution; the temperature of the air drying process is 10-40 o ℃.
And (3) carrying out anthocyanin dyeing on the air-dried lenses by adopting an integral soaking method, wherein the integral soaking method comprises the following steps of: soaking the air-dried lens in sterile ultrapure water for 60-120 minutes; completely immersing the lens in 5-30% (g/mL) anthocyanin aqueous solution with the pH of 7-8, and immersing at the temperature of 4-15 o ℃ for 1-2 days; taking out the colored lens, and putting the colored lens into sterile ultrapure water to be soaked for 20-200 minutes; and (5) cleaning the lens by using sterile ultrapure water to obtain the integral colored lens.
A silk fibroin color contact lens comprising a silk fibroin lens and anthocyanin bound to the silk fibroin lens; the silk fibroin lens is a porous silk fibroin film which is double-induced by acrylamide molecules, and the preparation method comprises the following steps: blending acrylamide molecules with a silk fibroin solution, inducing silk fibroin with an amorphous structure to form a porous silk fibroin film with stable crystallization, and removing acrylamide by repeated soaking and flushing; and dyeing the lens by adopting an anthocyanin molecular soaking method, so as to obtain the color contact lens with stable dyeing.
The invention has the beneficial effects that:
The main material components and the effects of the silk fibroin color contact lens provided by the invention are as follows:
Silk fibroin: an excellent natural high molecular fibrin has been widely used in the field of biomedical materials. As a natural biomaterial, silk fibroin possesses a number of remarkable superior properties, such as: good biocompatibility, proper mechanical property, low inflammatory reaction, good gas permeability and controllable degradation rate. Silk protein scaffolds are now widely used in a variety of tissue engineering, including repair of cartilage, nerves, blood vessels, and the like. In addition, the source is reliable, the acquisition is convenient, and the method is a great advantage of wide application.
Acrylamide: an organic compound is commonly used as a raw material for organic synthesis and a raw material for polymer materials. Is generally soluble in water and has good effect on flocculation of protein and starch in water. Has excellent flocculation property, thickening property, shearing resistance, resistance reduction, dispersibility and the like.
Anthocyanin: the water-soluble natural pigment widely existing in plants in nature has strong antioxidant, antimutagenic and antiallergic capabilities, and can strengthen vascular elasticity, improve circulatory system, improve sleep and inhibit inflammation and allergy. Meanwhile, the eye drop can be prevented, and the eye protection effect is achieved. It has certain inhibiting effect on elastase and collagenase, and can promote skin smoothness, delay aging, and prevent and treat skin injury caused by insolation. It is sensitive to the environment, and the pH value, the co-color effect and the like can influence the color development of the liquid crystal display.
In summary, the silk fibroin color contact lens provided by the invention has at least the following beneficial effects:
The safety is high: the silk fibroin and anthocyanin used for preparing the lenses are all derived from nature, have good biocompatibility and are safe to human bodies. The porous structure of the lens ensures that the lens has high oxygen permeability, can avoid the redness, swelling and pain of eyes caused by the oxygen deficiency of the cornea of eyes, and protects the health of eyeballs of a wearer.
Multifunctional: the silk fibroin color contact lens can play the role of anthocyanin active ingredients and prevent vision from being reduced. Based on the anthocyanin molecule active ingredient, the detection of the eye ball diseases can be realized.
Environmental protection: the silk fibroin and anthocyanin forming the lens can be degraded in natural environment, and the environment is not polluted.
Solves the technical problems of poor air permeability, hypoxia in cornea, redness, pain, dryness, even vision decline and the like of eyes caused by long-term wearing of the contact lens in the prior art.
Drawings
FIG. 1 is a flow chart of the preparation of a silk fibroin color contact lens in one embodiment of the present invention;
FIG. 2 is a schematic view in longitudinal section of an integrally colored silk fibroin color contact lens in one embodiment of the present invention.
Detailed Description
The invention adopts the following technical scheme: dissolving and dialyzing the crude silk fibroin product, and reacting with acrylamide in a mould to obtain the silk fibroin contact lens; the lenses are soaked and dyed by the prepared anthocyanin aqueous solution after being prepared.
As shown in fig. 1, the preparation process comprises the following main steps: mixing an acrylamide reagent with a silk fibroin solution; dripping the mixed solution into a contact lens mould, and air-drying and shaping; dyeing.
The preparation method of the lens comprises the following steps: and uniformly mixing the acrylamide solution and the silk fibroin solution, then dripping the mixture into a contact lens mold, and air-drying to obtain the lens.
The concentration of the silk fibroin solution is 5% -15% (g/mL), and the concentration of the acrylamide solution is 25% -75% (g/mL); and uniformly mixing the acrylamide solution and the silk fibroin solution according to the volume ratio of 1:10.
The silk fibroin crude product is put into sterile ultrapure water to be fully immersed, and is dissolved by ultrasonic waves of an ultrasonic cleaner; and dialyzing the solution for 3-5 days in sterile ultrapure water, and replacing the sterile ultrapure water every 6-24 hours.
The contact lens mold is cleaned and dried by sterile water, and then acrylamide and silk fibroin mixed solution is added; the temperature of the air drying process is 10-40 o ℃.
Carrying out anthocyanin dyeing on the air-dried lenses by adopting an integral soaking method;
The integral dip dyeing method comprises the following steps: soaking the air-dried lens in sterile ultrapure water for 60-120 minutes; completely immersing the lens in 5-30% (g/mL) anthocyanin aqueous solution with the pH of 7-8, and immersing at the temperature of 4-15 o ℃ for 1-2 days; taking out the colored lens, and putting the colored lens into sterile ultrapure water to be soaked for 20-200 minutes; and (5) cleaning the lens by using sterile ultrapure water to obtain the integral colored lens.
The silk fibroin color contact lens provided by the invention comprises a silk fibroin lens and anthocyanin combined on the silk fibroin lens. The silk fibroin lens is a porous silk fibroin film which is double-induced by acrylamide molecules, and the preparation method comprises the following steps: and (3) blending the acrylamide molecules with a silk fibroin solution, inducing the silk fibroin with an amorphous structure to form a porous silk fibroin film with stable crystallization, and removing the acrylamide by repeated soaking and washing. In order to successfully color the lens by anthocyanin molecules, the invention adopts an anthocyanin molecule soaking method to dye the lens, and in the method, the anthocyanin molecules and amino acid residues in silk fibroin have electrostatic adsorption physical action, so that the color contact lens with stable dyeing is obtained. The dyeing principle can improve the binding stability of anthocyanin molecules and silk fibroin on one hand and retain the physicochemical properties of the anthocyanin molecules on the other hand.
In the reaction of inducing the silk fibroin to form a film by the acrylamide molecule, the concentration of the silk fibroin is 5% -15%, and the concentration of the acrylamide is 2.5% -7.5%. In some embodiments, the temperature range of the acrylamide molecule for inducing the silk fibroin to form a film is 10-40 o ℃. In some embodiments, the humidity range of the acrylamide molecule induced silk fibroin film is 10% -50%. In some embodiments, the anthocyanin-tinted color lens is a unitary color lens. In some embodiments, the method of dyeing the integrally colored sheet is a dipping method.
The technical solutions of the present invention will be clearly and completely described with reference to the following examples, it being understood that the following examples are only for explaining the present invention and are not intended to limit the scope of the present invention.
The main reagent comprises:
Acrylamide is available from Shanghai Ala Biochemical technologies Co., ltd., product No. A108465-100g. Silk fibroin was purchased from summit biotechnology limited, cat No. LPSXKOA001. Anthocyanin was purchased from Shanghai Ala Biochemical technologies Co., ltd., cat# C302404-25g.
Preparing a solution:
2.5% (g/mL) acrylamide solution: 0.25g of acrylamide solid particles was weighed and added to 10mL of ultrapure water to prepare a 2.5% (g/mL) acrylamide solution.
5% (G/mL) silk fibroin solution: 2.5g of solid particles of silk fibroin were weighed and added to 50mL of ultrapure water to prepare a 5% (g/mL) silk fibroin solution.
5% (G/mL) of an aqueous anthocyanin solution (ph=7.5): 0.5g of anthocyanin solid particles are weighed and added into 10mL of sterile ultrapure water solution to prepare 10% (g/mL) anthocyanin aqueous solution.
Unless otherwise indicated, all reagents used in the examples below are conventional in the art and are commercially available or formulated according to conventional methods in the art and are of laboratory grade. Unless otherwise indicated, the experimental procedures and conditions used in the following examples are those conventional in the art and may be referred to the relevant experimental manuals, well-known literature or manufacturer's instructions. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
EXAMPLE 1 preparation of anthocyanin-dyed, integrally colored porous silk fibroin contact lenses
1. Preparation of porous silk fibroin lenses
Adding 0.1 mL of 25% (g/mL) acrylamide solution and 1 mL of 5% (g/mL) silk fibroin solution into a centrifuge tube of 5mL, uniformly mixing, dripping 100 mu L of the mixture into a contact lens mold, placing the contact lens mold at 10-40 ℃ for air drying at a ventilation place to obtain sterile ultrapure water of the silk fibroin lens, soaking for 60 minutes, removing the soaking solution, adding sterile ultrapure water for cleaning for 30 minutes, repeatedly cleaning for three times, and finally placing the cleaned silk fibroin lens into sterile physiological saline for standby.
2. Anthocyanin staining
Placing the silk fibroin lens prepared and cleaned in the step 1 into a centrifuge tube (pH=7.5) filled with 5% (g/mL) anthocyanin aqueous solution, placing into a 4 o C refrigerator, taking out the silk fibroin lens dyed by anthocyanin after 1 day, placing into sterile ultrapure water for soaking for 30 minutes, removing the soaking liquid, adding the sterile ultrapure water for cleaning, repeatedly cleaning for three times, and finally placing the cleaned anthocyanin-dyed lens into sterile physiological saline for standby.
Example 2 preparation of anthocyanin-dyed Ring-shaped colored silk fibroin contact lenses
1. Preparation of porous silk fibroin lenses
Adding 0.1 mL of 50% (g/mL) acrylamide solution and 1 mL of 15% (g/mL) silk fibroin solution into a centrifuge tube of 5mL, uniformly mixing, dripping 100 mu L of the mixture into a contact lens mold, placing the contact lens mold at 10-40 ℃ for air drying at a ventilation place to obtain sterile ultrapure water of the silk fibroin lens, soaking for 80 minutes, removing the soaking solution, adding the sterile ultrapure water for cleaning for 30 minutes, repeatedly cleaning for three times, and finally placing the cleaned silk fibroin lens into sterile physiological saline for standby.
2. Anthocyanin staining
Placing the silk fibroin lens prepared and cleaned in the step 1 into a centrifuge tube (pH=7.5) filled with 10% (g/mL) anthocyanin aqueous solution, placing into a 4 o C refrigerator, taking out the silk fibroin lens dyed by anthocyanin after 1 day, placing into sterile ultrapure water for soaking for 30 minutes, removing the soaking liquid, adding the sterile ultrapure water for cleaning, repeatedly cleaning for three times, and finally placing the cleaned anthocyanin-dyed lens into sterile physiological saline for standby.
Claims (3)
1. A preparation method of a silk fibroin color contact lens is characterized by comprising the following steps: dissolving and dialyzing a crude silk fibroin product, and then reacting with acrylamide in a mould to prepare the silk fibroin contact lens; soaking and dyeing the lens by using the prepared anthocyanin aqueous solution after the lens is prepared; uniformly mixing an acrylamide solution and a silk fibroin solution, then dripping the mixture into a contact lens mold, and air-drying to obtain a lens; the concentration of the silk fibroin solution is 5% -15% g/mL, and the concentration of the acrylamide solution is 25% -75% g/mL; uniformly mixing an acrylamide solution and a silk fibroin solution according to a volume ratio of 1:10; cleaning and drying a contact lens mold by using sterile water, and then adding an acrylamide and silk fibroin mixed solution; the temperature of the air drying process is 10-40 o ℃; and (3) carrying out anthocyanin dyeing on the air-dried lenses by adopting an integral soaking method, wherein the integral soaking method comprises the following steps of: soaking the air-dried lens in sterile ultrapure water for 60-120 minutes; completely immersing the lens in 5-30% g/mL anthocyanin aqueous solution with pH of 7-8 at 4-15 o ℃ for 1-2 days; taking out the colored lens, and putting the colored lens into sterile ultrapure water to be soaked for 20-200 minutes; and (5) cleaning the lens by using sterile ultrapure water to obtain the integral colored lens.
2. The method for preparing the silk fibroin color contact lens according to claim 1, wherein the method comprises the following steps: the method for dissolving and dialyzing the crude silk fibroin product comprises the following steps: the silk fibroin crude product is put into sterile ultrapure water to be fully immersed, the sterile ultrapure water is dissolved by ultrasonic cleaner, the solution is dialyzed for 3-5 days in the sterile ultrapure water, and the sterile ultrapure water is replaced every 6-24 hours.
3. The method for preparing the silk fibroin color contact lens according to claim 1, wherein the method comprises the following steps: a silk fibroin color contact lens comprising a silk fibroin lens and anthocyanin bound to the silk fibroin lens; the silk fibroin lens is a porous silk fibroin film which is double-induced by acrylamide molecules, and the preparation method comprises the following steps: blending acrylamide molecules with a silk fibroin solution, inducing silk fibroin with an amorphous structure to form a porous silk fibroin film with stable crystallization, and removing acrylamide by repeated soaking and flushing; and dyeing the lens by adopting an anthocyanin molecular soaking method, so as to obtain the color contact lens with stable dyeing.
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| CN202210949220.3A CN115339135B (en) | 2022-08-09 | 2022-08-09 | A method for preparing silk fibroin colored contact lenses |
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| CN115128839A (en) * | 2022-08-09 | 2022-09-30 | 重庆大学 | Method for preparing silk fibroin contact lenses based on natural silk |
| CN115327796A (en) * | 2022-08-09 | 2022-11-11 | 重庆大学 | Blue-light-proof organic contact lens and preparation method thereof |
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| JP2740822B2 (en) * | 1992-04-08 | 1998-04-15 | 工業技術院長 | Contact lens and manufacturing method thereof |
| JP3629571B2 (en) * | 1996-05-28 | 2005-03-16 | 独立行政法人農業生物資源研究所 | Method for producing silk fibroin lump and contact lens comprising the lump |
| JP3936999B2 (en) * | 1997-08-07 | 2007-06-27 | 独立行政法人農業生物資源研究所 | Contact lens containing natural biopolymer and method for producing the same |
| CN1394875A (en) * | 2001-07-11 | 2003-02-05 | 中国科学院化学研究所 | Method for extracting fimbrin |
| JP3906927B2 (en) * | 2003-03-14 | 2007-04-18 | 独立行政法人農業生物資源研究所 | Production and utilization of functional protein derived from silk protein |
| JP2005052162A (en) * | 2003-04-10 | 2005-03-03 | Silk Kogei:Kk | Method for producing silk fibroin film |
| US20110008436A1 (en) * | 2009-04-20 | 2011-01-13 | Altman Gregory H | Silk Fibroin Hydrogels and Uses Thereof |
| RU2443805C1 (en) * | 2010-08-20 | 2012-02-27 | Государственное образовательное учреждение высшего профессионального образования "Санкт-Петербургский государственный университет технологии и дизайна" (СПГУТД) | Method of producing silk fibroin based film for making contact lenses |
| CN104693298A (en) * | 2015-02-16 | 2015-06-10 | 李志强 | Technique for industrialized and batch collection of liquid silk in various silkworm silk-glands |
| US10857262B2 (en) * | 2016-10-31 | 2020-12-08 | Sofregen Medical, Inc. | Compositions comprising low molecular weight silk fibroin fragments and plasticizers |
| TWI593995B (en) * | 2016-12-09 | 2017-08-01 | 國立虎尾科技大學 | Preparation of Dye-free Colored Contact Lens with Silk Fibroin Protein and Its Products |
| WO2019182905A1 (en) * | 2018-03-23 | 2019-09-26 | Arch Biosurgery, Inc. | Sap and peptidomimetics for treatment of eye disease |
| TWI670303B (en) * | 2018-11-14 | 2019-09-01 | 國立虎尾科技大學 | Anti-ultraviolet biodegradable transparent film based on bionic structure and method fabricating the same |
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|---|---|---|---|---|
| CN115128839A (en) * | 2022-08-09 | 2022-09-30 | 重庆大学 | Method for preparing silk fibroin contact lenses based on natural silk |
| CN115327796A (en) * | 2022-08-09 | 2022-11-11 | 重庆大学 | Blue-light-proof organic contact lens and preparation method thereof |
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