CN116271237A - A kind of breast autologous collagen filler preparation method - Google Patents

Abstract

The invention discloses a chest autologous collagen filler preparation method, which relates to the field of collagen fillers and solves the problems that the existing cellulite is easy to be absorbed in a body and cannot achieve an effect, and the invention provides a scheme that the method comprises the steps of culturing extracted autologous fat particle cells to 90% of fusion degree, sucking out an original culture medium, replacing the culture medium for culturing for 48 hours, concentrating filtrate to 1000mg/L of protein concentration, and obtaining the culture medium for continuous culture; extracting fat after culturing, centrifuging, wherein the upper layer of the injector is fat drop, the lower layer is reddish swelling liquid and blood, the middle layer is purified fat after centrifuging and concentrating, and the layer is observed, and 1/4 of purified fat is slightly dense and whitish, namely high-density fat; taking 3/4 layers of fat after centrifugation, and performing low-oxygen culture and freezing preservation. The method has the advantages that the activity rate of the filler prepared by the method is obviously improved, and the one-time filling effect is also greatly improved.

Description

A kind of breast autologous collagen filler preparation method

technical field

The invention relates to the field of collagen fillers, in particular to a method for preparing breast autologous collagen fillers.

Background technique

Breast enlargement, also known as breast augmentation or breast enlargement, is a kind of breast enlargement behavior performed by women in order to enhance their personal femininity. It mainly relies on external force to shape the shape of the breast. The common breast enhancement methods are: food breast enhancement, that is, by adjusting the diet, borrowing the nutrients in vegetables to achieve the purpose of breast enhancement; drug breast enhancement, that is, using or taking drugs for breast enhancement; surgical breast enhancement, filling the breast with silica gel and other products to achieve the purpose of breast enhancement, breast augmentation The operation needs to cut out the wound to put in the stuffing. Of course, this wound must be concealed to avoid being discovered. Therefore, many people choose to operate under the armpit, in the areola or under the breast for beautiful wounds. Experts suggest that sports breast enhancement can be used to achieve the ideal bust.

There are currently two main methods of breast enhancement on the market, namely: breast augmentation with prosthesis and breast augmentation with fat. Prosthesis breast augmentation is formed at one time, but the prosthesis itself has a lifespan and many side effects, such as: may cause hematoma, prosthesis rupture, capsular contracture, breast hardening, paresthesia, abnormal position caused by improper operation, and chest X-ray ray will be blocked.

Fat breast augmentation is to inject untreated cellulite into the breast, but the injected cellulite is easily absorbed in the body and cannot achieve the effect. Therefore, a method for preparing breast autologous collagen filler is proposed.

Contents of the invention

The purpose of the present invention is to provide a method for preparing breast autologous collagen filler, which solves the problem that the existing cellulite is easily absorbed in the body and cannot achieve the effect.

In order to achieve the above object, the present invention provides the following technical scheme: a method for preparing breast autologous collagen filler, comprising the following steps:

S1: Use a special liposuction blunt tube and a 20ml syringe to manually extract fat particles in a low-speed, continuous, fan-shaped manner, and discard the water after standing;

S2: Culture the extracted autologous fat granule cells to a confluence of 90%, suck out the original medium, replace the medium and culture for 48 hours, concentrate the filtrate to a protein concentration of 1000mg/L, obtain the medium and continue to cultivate;

S3: Extract fat after cultivation, and perform centrifugation. After centrifugation, the upper layer of the syringe can be seen as fat droplets, the lower layer is light red swelling fluid and blood, and the middle layer is the purified fat after centrifugal concentration. Observe this layer, see below 1/4 of the purified fat is slightly dense and whitish, which is high-density fat;

S4: take the upper 3/4 layer of fat after centrifugation, culture in hypoxia, and cryopreserve;

S5: Put fat particles into a micro-stirrer and stir at a high speed to make nano-fat.

Preferably, the centrifugation operation in S3 is performed by a low-speed centrifuge, and its operating conditions are: 1200G centrifugal force, 3 minutes.

Preferably, the rotating speed of the micro-stirrer in S5 is 120 rpm, and the stirring time is 20-30 minutes.

Compared with related technologies, a method for preparing breast autologous collagen filler provided by the present invention has the following beneficial effects:

1. The present invention provides a method for preparing breast autologous collagen fillers. By transplanting high-density fat (deep layer) combined with nano-fat technology (shallow layer) to correct tear trough deformities, the survival rate of fat is significantly improved, and it is also greatly improved once. fill effect.

Detailed ways

The technical solution will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them; based on the embodiments of the present invention, those skilled in the art All other embodiments obtained by the skilled person without creative work belong to the protection scope of the present invention.

Embodiment one:

The present invention provides a technical solution: a method for preparing breast autologous collagen filler, comprising the following steps:

S1: Use a special liposuction blunt tube and a 20ml syringe to manually extract fat particles in a low-speed, continuous, fan-shaped manner, and discard the water after standing;

S2: Culture the extracted autologous fat granule cells to a confluence of 90%, suck out the original medium, replace the medium and culture for 48 hours, concentrate the filtrate to a protein concentration of 1000mg/L, obtain the medium and continue to cultivate;

S3: Extract fat after cultivation, and perform centrifugation. After centrifugation, the upper layer of the syringe can be seen as fat droplets, the lower layer is light red swelling fluid and blood, and the middle layer is the purified fat after centrifugal concentration. Observe this layer, see below 1/4 of the purified fat is slightly dense and whitish, which is high-density fat;

S4: Take the upper 3/4 layer of fat after centrifugation, culture in hypoxia, and cryopreserve;

S5: Put fat particles into a micro-stirrer and stir at a high speed to make nano-fat.

The centrifugation operation in S3 is carried out by a low-speed centrifuge, and its operating conditions are: 1200G centrifugal force, 3 minutes.

The rotating speed of the micro-stirrer in S5 is 120 rpm, and the stirring time is 20-30 minutes.

In this embodiment, a dedicated fat injection blunt tube (length 6 cm, inner diameter 1.2 mm) is selected to be connected to a 1 ml threaded syringe. We generally inject high-density fat in the superficial periosteum, inject nano-fat in the superficial subcutaneous layer, and inject layered three-dimensionally.

Select the middle part of the cheek for the superficial layer of the periosteum, use the left hand to first determine the position of the orbital bone margin, and enter vertically, and the superficial layer of the periosteum will feel empty when advancing, indicating that the orbital septum is touched, and the injection is performed slowly along the orbital rim; each cellulite is not more than 0.05ml ;From far to near, injection while withdrawing, and slow injection, multi-point, small amount, multi-level, and multi-tunnel cross-injection is carried out, so that the injected high-density fat can be evenly distributed in the entire recipient area, and it can be naturally connected with the surrounding tissue. Integration until the tear trough depression is basically flattened. Generally, high-density fat injection is 1.2-3.0m1 on each side.

After the deep high-density fat filling is completed, the surface of the lower eyelid skin generally has a slight depression, which can be filled with subcutaneous superficial nano-fat in the depression. Needle entry point: the intersection of the horizontal line of the infraorbital rim and the vertical line of the lateral canthus. Thin blunt fat injection needle, level: the superficial layer of the orbicularis oculi muscle sneaks horizontally, reaching the innermost side of the lower eyelid (pay attention to avoiding the inner canthal vein), radially withdraws the needle while injecting, milky fine-grained fat, evenly spreads on the eye wheel in the sunken area For the superficial layer of the orbicularis muscle, do not inject too much or protrude, and the volume of each injection should not exceed 0.02ml; generally, the volume of unilateral injection should be less than 0.8ml.

The surgical area was not bandaged. The needle port is pasted with sterile adhesive tape, removed within 24 hours, the injection needle port is kept dry, and erythromycin eye ointment is applied externally, and it heals after 5 days and can be soaked in water.

The patients in this group were followed up for 2 to 18 months, with an average follow-up of 6 months. There was no fat infection, liquefaction, cyst formation or other serious complications. 35 patients were significantly improved after one operation. The fat in the tear trough filling area was full, the surface skin was firm and elastic, the skin pigment was significantly reduced, and the vision was younger and the improvement was obvious. After the first operation, 7 patients still hoped for further improvement subjectively, or found mild atrophy of fat in the transplanted area during follow-up, etc., 3 to 6 months later, 2 more filling operations were performed, and finally they were basically satisfied. Five patients had tear trough swelling for more than 3 months after operation, and basically recovered half a year later.

Claims (3)

1. The preparation method of the chest autologous collagen filler is characterized by comprising the following steps of:

s1: manually extracting fat particles at low speed, continuously and in a fan shape by using a special fat sucking blunt tube and a 20 ml syringe, standing and removing water;

s2: culturing the extracted autologous fat granule cells to 90% of fusion degree, sucking out the original culture medium, replacing the culture medium for culturing for 48h, concentrating the filtrate until the protein concentration is 1000mg/L, and obtaining the culture medium for continuous culture;

s3: extracting fat after culturing, centrifuging, wherein the upper layer of the injector is fat drop, the lower layer is reddish swelling liquid and blood, the middle layer is purified fat after centrifuging and concentrating, and the layer is observed, and 1/4 of purified fat is slightly dense and whitish, namely high-density fat;

s4: taking 3/4 layers of fat after centrifugation, and performing hypoxia culture and freeze preservation;

s5: and (3) placing the fat particles into a micro-stirrer, and stirring at a high speed to obtain the nano fat.

2. The method for preparing the autologous chest collagen filling according to claim 1, wherein the centrifugation operation in S3 is performed by a low-speed centrifuge under the following operating conditions: centrifugal force of 1200G, 3 minutes.

3. The method for preparing the autologous chest collagen filling according to claim 1, wherein the rotation speed of the mini-mixer in the step S5 is 120 revolutions and the mixing time is 20-30 minutes.