CN118441414A

CN118441414A - Silk fibroin peptide nanofiber mask and preparation method thereof

Info

Publication number: CN118441414A
Application number: CN202410368462.2A
Authority: CN
Inventors: 周宁; 堐梦娜; 倪婧怡; 董伊航; 张文凯; 张克勤
Original assignee: Suzhou Best Color Nanotechnology Co ltd
Current assignee: Suzhou Best Color Nanotechnology Co ltd
Priority date: 2024-03-28
Filing date: 2024-03-28
Publication date: 2024-08-06

Abstract

The invention relates to a silk fibroin peptide nanofiber facial mask and a preparation method thereof, and belongs to the technical field of facial masks. The raw materials of the silk fibroin peptide nanofiber facial mask comprise, by weight, 10% -15% of a water-soluble polymer, 0.5% -3% of silk fibroin peptide, 0.5% -3% of an active substance, 3% -5% of a polysaccharide substance, 2% -5% of a thickening substance and the balance of water; the silk fibroin peptide nanofiber facial mask is prepared by a double-needle electrostatic spinning technology. The preparation method reduces the influence of voltage environment, temperature and solvent on the silk fibroin peptide by a double-needle electrostatic spinning technology, reduces the damage of the pH value of the solution to the silk fibroin peptide, and protects the silk fibroin peptide to play a role after the silk fibroin peptide is dissolved quickly on the face.

Description

Silk fibroin peptide nanofiber mask and preparation method thereof

Technical Field

The invention belongs to the technical field of masks, and particularly relates to a silk fibroin peptide nanofiber mask and a preparation method thereof.

Background

Silk Fibroin (SF) is a natural structural protein secreted from salivary glands and pupae of insects of the arachnidae family such as silk. It has excellent biocompatibility, biodegradability, adjustable form and structure, etc. and is thus widely used in medicine, tissue engineering, medicine delivery, textile, food, etc.

The silk fibroin contains 18 amino acids such as serine, glutamic acid, glycine and the like, can effectively prevent water loss after being formed into a film on the surface of skin, has a moisturizing effect, and therefore has strong advantages and development space in the field of beauty and make-up. However, the molecular weight of general silk fibroin is large (200 kDa-250 kDa), and these macromolecular proteins are difficult to be directly absorbed by human body. If silk fibroin peptide with smaller molecular weight is selected, the absorption of skin to the silk fibroin peptide can be effectively promoted.

The silk fibroin peptide is degradation product of silk fibroin, silk fibroin fiber is dissolved in salt solution, neutral protease is added, enzyme is deactivated in a low-temperature freezing state through enzymolysis reaction, stirring and hydrolysis according to a certain proportion, and the silk fibroin peptide is obtained after filtration and vacuum drying. A silk fibroin peptide is a biochemical substance between amino acids and proteins, has a smaller molecular weight than silk fibroin and a larger molecular weight than amino acids, and is a fragment of silk fibroin. Has the characteristics of small molecular weight, easy absorption, good stability, good solubility and the like, and can directly permeate into the dermis layer to play a role. In addition, the silk fibroin peptide is similar to the facial skin components of human, is nontoxic and harmless to human tissues, permeates into the skin epidermis layer during the process of pasting the facial mask, and can effectively supplement the missing nutritional components. The micromolecular silk fibroin peptide can promote degradation of collagen disordered by aging through various ways, and stimulates fibroblasts of a dermis layer to synthesize more fresh regular collagen to replace the collagen, so that growth of the fibroblasts is promoted, and skin can be well improved.

However, in the early stage, the silk fibroin is dissolved by using inorganic acid as a solvent, and the silk fibroin can be dissolved, but the damage to the silk fibroin is serious, the silk fibroin is degraded into small molecular peptide chains, the formed silk fibroin peptide has almost no mechanical property, and the film prepared from the silk fibroin peptide has small strength, compactness, no pore and poor supporting property. Subsequent modification methods have not been able to improve the mechanical properties of silk fibroin peptides. In addition, silk fibroin peptides are susceptible to temperature and solvents, limiting the application of silk fibroin peptides in the cosmetic field.

Disclosure of Invention

In order to solve the technical problems, the invention provides a silk fibroin peptide nanofiber facial mask and a preparation method thereof.

The first object of the invention is to provide a silk fibroin peptide nanofiber facial mask, which comprises, by weight, 10% -15% of a water-soluble polymer, 0.5% -3% of silk fibroin peptide, 0.5% -3% of an active substance, 3% -5% of a polysaccharide substance, 2% -5% of a thickening substance and the balance of water; the silk fibroin peptide nanofiber facial mask is prepared by a double-needle electrostatic spinning technology.

In one embodiment of the invention, the water-soluble polymer is selected from one or more of hydrogenated starch, hydroxymethyl cellulose, ethylene oxide PEO, polyvinylpyrrolidone PVP, and polyvinyl alcohol PVA.

In one embodiment of the invention, the active substance is selected from one or more of sodium hyaluronate, nicotinamide, retinol and derivatives thereof.

Further, the active substance is sodium hyaluronate, which is a polysaccharide substance naturally occurring in human tissue and is also a common skin care ingredient. It has strong moisture keeping ability and water keeping ability, can absorb and lock moisture, increase moisture content of skin, and keep skin soft, smooth and elastic.

In one embodiment of the invention, the molecular weight of the silk fibroin peptide is 500Da-2000Da, and the silk fibroin peptide has natural affinity effect with skin and is easily absorbed by the skin.

In one embodiment of the present invention, the polysaccharide is selected from one or more of sodium alginate, chitosan and mannose.

Further, the polysaccharide substance is selected from sodium alginate and/or chitosan. The sodium alginate can change the cohesiveness of the tissue, so that the tensile force is strong, the bending degree is large, and the hydrophilic property of the sodium alginate enables the sodium alginate to have high moisturizing property. Chitosan is a hydrophilic polysaccharide which is formed by deacetylating chitin and structurally connecting D-glucosamine by beta-1, 4-glycosidic bond, has a large number of amino groups on molecular side chains, has the characteristics of biodegradability, cell affinity, certain bacteriostasis, no toxicity, anticancer and the like, and can promote tissue repair, inhibit scar hyperplasia and regulate immune function.

In one embodiment of the invention, the thickening material is selected from one or more of gum arabic, gelatin and xanthan gum.

Further, the thickening substance is gelatin, the gelatin is a natural protein derived from collagen hydrolysis, and has very similar molecular structure and function with collagen, and better biological activity. Gelatin has a large number of hydroxyl groups, amino groups and carboxyl groups in the molecule, which makes the electrospun film itself which uses it as a main raw material have extremely strong hydrophilicity. In addition, gelatin has better degradability, good biocompatibility, better fibrosis and other physical and chemical properties, and is often used for preparing tissue engineering scaffold materials as carriers for cell and tissue culture, so that the gelatin is a popular biological material for clinical medicine and biomedical theory research.

The second object of the invention is to provide a preparation method of the silk fibroin peptide nanofiber facial mask, which comprises the following steps:

s1, dissolving a water-soluble polymer, silk fibroin peptide, an active substance, a polysaccharide substance and a thickening substance in water, uniformly stirring, standing and defoaming to obtain spinning solution;

s2, preparing the spinning solution in the step S1 into the silk fibroin peptide nanofiber facial mask through a double-needle electrostatic spinning technology.

In one embodiment of the present invention, in S1, the water-soluble polymer has a dissolution temperature of 70 ℃ to 80 ℃ and a stirring time of 2h to 4h; the dissolution temperature of the silk fibroin peptide, the active substances, the polysaccharide substances and the thickening substances is 70-80 ℃, and the stirring time is independently 4-6 h.

In one embodiment of the present invention, in S1, the time for the standing and defoaming is 5h to 8h.

In one embodiment of the present invention, in S2, the process conditions of the twin-needle electrospinning are: the first voltage is 10kV-20kV, the second voltage is 15kV-25kV, the sample injection rate is 1mL/h-2mL/h, the temperature is 15-30 ℃, and the humidity is 20-30%.

In one embodiment of the invention, in S2, the receiving substrate during double needle electrospinning is selected from a nonwoven fabric, a gauze, or a release paper.

In one embodiment of the invention, in S2, the double-needle electrostatic spinning technology is to generate taylor cone between the steel wire and the base cloth conducting the spinning solution while fast moving through an electric field generated by high pressure, and the polymer liquid drops are sprayed into trickles against the surface tension and then converted into curled spiral fibers to prepare the silk fibroin peptide nanofiber membrane.

Compared with the prior art, the technical scheme of the invention has the following advantages:

(1) According to the preparation method disclosed by the invention, water is used as a solvent, the water-soluble polymer and the polysaccharide substance are mixed, and the spinnability of the spinning solution is greatly improved through intermolecular interaction of hydrogen bonds, so that uniform and continuous nanofiber electrostatic spinning is realized, the prepared silk fibroin peptide nanofiber mask can keep good biological properties, a good moisturizing effect is achieved, and an anti-wrinkle effect is achieved to a certain extent. In addition, after the water-soluble polymer is mixed with the silk fibroin peptide, the water-soluble polymer plays a role in leading the mechanical strength of the fiber membrane, so that the mechanical property of the silk fibroin peptide fiber membrane can be improved, and the elasticity of the membrane can be enhanced.

(2) According to the preparation method, the spinning solution is prepared into the silk fibroin peptide nanofiber facial mask with high specific surface area, small diameter, small pore diameter, large porosity and strong uniformity by a double-needle electrostatic spinning technology. The mask prepared by the electrostatic spinning technology can ensure the maximum fitting between the mask and the skin surface, can be well adhered to the face, is softer than the traditional mask, and can release nutrients to the skin surface more easily, thereby being beneficial to enhancing the skin permeability and restoring the youthful state of the skin. In addition, the electrostatic spinning technology mostly adopts organic reagent as solvent, and can bring additional adverse effect when being used in cosmetics. The silk fibroin peptide nanofiber facial mask can accelerate the release of silk fibroin peptide under the assistance of water or essence, so as to achieve the purpose of rapid skin care.

(3) The preparation method reduces the influence of voltage environment, temperature and solvent on the silk fibroin peptide by a double-needle electrostatic spinning technology, reduces the damage of the pH value of the solution to the silk fibroin peptide, and protects the silk fibroin peptide from being effective after the facial instant dissolution.

Drawings

In order that the invention may be more readily understood, a more particular description of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings, in which:

FIG. 1 is a schematic diagram of a double needle electrostatic spinning apparatus of the present invention;

FIG. 2 shows the microstructure of the silk fibroin peptide nanofiber mask of example 3 of the present invention at 500 Xmagnification in an electron scanning microscope;

FIG. 3 shows the microstructure of the silk fibroin peptide nanofiber mask of example 3 of the present invention at 2000 Xmagnification in an electron scanning microscope;

FIG. 4 is an electron scanning microscope image of a silk fibroin peptide nanofiber mask of comparative example 1 of the present invention;

FIG. 5 is an electron scanning microscope image of a silk fibroin peptide nanofiber mask of comparative example 2 of the present invention;

FIG. 6 is a graph showing the distribution of fiber diameters of a silk fibroin peptide nanofiber mask in accordance with example 3 of the present invention;

FIG. 7 shows the absorption test result of test example 3 according to the present invention.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and specific examples, which are not intended to be limiting, so that those skilled in the art will better understand the invention and practice it.

In the present invention, the molecular weight of the silk fibroin peptides used in examples and comparative examples was about 1200Da unless otherwise specified.

Example 1

The silk fibroin peptide nanofiber facial mask and the preparation method thereof specifically comprise the following steps:

s1, taking 10% hydrogenated starch in deionized water, heating to 75 ℃, and stirring for 3 hours until the hydrogenated starch is completely dissolved; after the temperature is reduced to 30 ℃, 0.5% of silk fibroin peptide, 1% of sodium hyaluronate, 3% of chitosan and 2% of gelatin are added, and after the mixture is fully stirred for 5 hours, the mixture is left to stand for defoaming for 6 hours, so that uniform spinning solution is formed;

S2, preparing a silk fibroin peptide nanofiber membrane from the spinning solution through double-needle electrostatic spinning equipment (figure 1); the electrostatic spinning conditions are as follows: the first voltage is 15kV, the second voltage is 20kV, and the sample injection rate is 1mL/h. The ambient temperature is controlled at 25 ℃, the humidity is 30%, and the receiving substrate is non-woven fabric.

Example 2

S1, taking PEO with the mass concentration of 10% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, 0.5% of silk fibroin peptide, 1% of sodium hyaluronate, 3% of chitosan and 2% of gelatin are added, and after the mixture is fully stirred for 5 hours, the mixture is left to stand for defoaming for 6 hours, so that uniform spinning solution is formed;

Example 3

s1, taking PVA with the mass concentration of 10% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PVA is completely dissolved; after the temperature is reduced to 30 ℃, 0.5% of silk fibroin peptide, 1% of sodium hyaluronate, 3% of chitosan and 2% of gelatin are added, and after the mixture is fully stirred for 5 hours, the mixture is left to stand for defoaming for 6 hours, so that uniform spinning solution is formed;

Example 4

S1, PVP with the mass concentration of 10% is taken and heated to 75 ℃ in deionized water, and stirred for 3 hours until the PVP is completely dissolved; after the temperature is reduced to 30 ℃, 0.5% of silk fibroin peptide, 1% of sodium hyaluronate, 3% of chitosan and 2% of gelatin are added, and after the mixture is fully stirred for 5 hours, the mixture is left to stand for defoaming for 6 hours, so that uniform spinning solution is formed;

Example 5

S1, taking PEO with the mass concentration of 10% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 1% of silk fibroin peptide, 1% of sodium hyaluronate, 3% of chitosan and 2% of gelatin, fully stirring for 5 hours, and standing for defoaming for 6 hours to form uniform spinning solution;

Example 6

S1, taking PEO with the mass concentration of 10% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 2% of silk fibroin peptide, 1% of sodium hyaluronate, 3% of chitosan and 2% of gelatin, fully stirring for 5 hours, and standing for defoaming for 6 hours to form uniform spinning solution;

Example 7

s1, taking PEO with the mass concentration of 10% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 1% of silk fibroin peptide, 1.5% of sodium hyaluronate, 3% of chitosan and 2% of gelatin, fully stirring for 5 hours, and standing for defoaming for 6 hours to form uniform spinning solution;

Example 8

S1, taking PEO with the mass concentration of 10% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 1% of silk fibroin peptide, 2% of sodium hyaluronate, 3% of chitosan and 2% of gelatin, fully stirring for 5 hours, and standing for defoaming for 6 hours to form uniform spinning solution;

Example 9

s1, taking PEO with the mass concentration of 10% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 1% of silk fibroin peptide, 2% of sodium hyaluronate, 3% of sodium alginate and 2% of gelatin, fully stirring for 5 hours, standing and defoaming for 6 hours to form uniform spinning solution;

Example 10

s1, taking PEO with the mass concentration of 10% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 1% of silk fibroin peptide, 2% of sodium hyaluronate, 3% of mannose and 2% of gelatin, fully stirring for 5 hours, standing for deaeration for 6 hours, and forming uniform spinning solution;

Example 11

S1, taking PEO with the mass concentration of 10% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 1% of silk fibroin peptide, 2% of sodium hyaluronate, 3% of sodium alginate and 2% of Arabic gum, fully stirring for 5 hours, standing for defoaming for 6 hours, and forming uniform spinning solution;

Example 12

s1, taking PEO with the mass concentration of 10% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 1% of silk fibroin peptide, 2% of sodium hyaluronate, 3% of sodium alginate and 2% of xanthan gum, fully stirring for 5 hours, standing and defoaming for 6 hours to form uniform spinning solution;

Example 13

S1, taking PEO with the mass concentration of 11% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 1% of silk fibroin peptide, 2% of sodium hyaluronate, 3% of sodium alginate and 2% of gelatin, fully stirring for 5 hours, standing and defoaming for 6 hours to form uniform spinning solution;

Example 14

S1, taking PEO with the mass concentration of 12% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 1% of silk fibroin peptide, 2% of sodium hyaluronate, 3% of sodium alginate and 2% of gelatin, fully stirring for 5 hours, standing and defoaming for 6 hours to form uniform spinning solution;

Example 15

S1, taking PEO with the mass concentration of 13% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PEO is completely dissolved; after the temperature is reduced to 30 ℃, adding 1% of silk fibroin peptide, 2% of sodium hyaluronate, 3% of sodium alginate and 2% of gelatin, fully stirring for 5 hours, standing and defoaming for 6 hours to form uniform spinning solution;

Example 16

s2, preparing a silk fibroin peptide nanofiber membrane from the spinning solution through double-needle electrostatic spinning equipment (figure 1); the electrostatic spinning conditions are as follows: the first voltage is 15kV, the second voltage is 22kV, and the sample injection rate is 1mL/h. The ambient temperature is controlled at 25 ℃, the humidity is 30%, and the receiving substrate is non-woven fabric.

Example 17

S2, preparing a silk fibroin peptide nanofiber membrane from the spinning solution through double-needle electrostatic spinning equipment (figure 1); the electrostatic spinning conditions are as follows: the first voltage is 15kV, the second voltage is 25kV, and the sample injection rate is 1mL/h. The ambient temperature is controlled at 25 ℃, the humidity is 30%, and the receiving substrate is non-woven fabric.

Example 18

S2, preparing a silk fibroin peptide nanofiber membrane from the spinning solution through double-needle electrostatic spinning equipment (figure 1); the electrostatic spinning conditions are as follows: the first voltage is 15kV, the second voltage is 25kV, and the sample injection rate is 1mL/h. The ambient temperature is controlled at 25 ℃, the humidity is 35%, and the receiving substrate is non-woven fabric.

Example 19

S2, preparing a silk fibroin peptide nanofiber membrane from the spinning solution through double-needle electrostatic spinning equipment (figure 1); the electrostatic spinning conditions are as follows: the first voltage is 15kV, the second voltage is 25kV, and the sample injection rate is 1mL/h. The ambient temperature is controlled at 25 ℃, the humidity is 40%, and the receiving substrate is non-woven fabric.

Example 20

s2, preparing a silk fibroin peptide nanofiber membrane from the spinning solution through double-needle electrostatic spinning equipment (figure 1); the electrostatic spinning conditions are as follows: the first voltage is 15kV, the second voltage is 25kV, and the sample injection rate is 1mL/h. The ambient temperature was controlled at 25 ℃ and the humidity at 30% with the receiving substrate being a gauze.

Example 21

S2, preparing a silk fibroin peptide nanofiber membrane from the spinning solution through double-needle electrostatic spinning equipment (figure 1); the electrostatic spinning conditions are as follows: the first voltage is 15kV, the second voltage is 20kV, and the sample injection rate is 1mL/h. The ambient temperature is controlled at 25 ℃, the humidity is 30%, and the receiving base material is release paper.

Comparative example 1 substantially the same as example 3, except that the concentration of PVA was changed

S1, taking PVA with the mass concentration of 30% in deionized water, heating to 75 ℃, and stirring for 3 hours until the PVA is completely dissolved; after the temperature is reduced to 30 ℃, 0.5% of silk fibroin peptide, 1% of sodium hyaluronate, 3% of chitosan and 2% of gelatin are added, and after the mixture is fully stirred for 5 hours, the mixture is left to stand for defoaming for 6 hours, so that uniform spinning solution is formed;

Comparative example 2 substantially the same as example 3, except for the electrospinning conditions

S2, preparing a silk fibroin peptide nanofiber mask from the spinning solution through double-needle electrostatic spinning equipment; the electrostatic spinning conditions are as follows: the first voltage is 20kV, the second voltage is 30kV, and the sample injection rate is 1mL/h. The ambient temperature is controlled at 25 ℃, the humidity is 30%, and the receiving substrate is non-woven fabric.

Test example 1

The silk fibroin peptide nanofiber masks prepared in example 3 and comparative examples 1-2 were characterized and the results are shown in fig. 2-5. From fig. 2-3, it can be seen that the silk fibroin peptide nanofiber membrane has uniform fiber distribution and no beading and agglomeration. As can be seen from fig. 4 to 5, the fiber diameter of the silk fibroin peptide nanofiber membrane becomes large, uniformity becomes poor, and beaded or beaded nanofibers appear. The result of comparative example 1 is that the viscosity and the external tension become large due to the excessively high concentration of the water-soluble polymer, resulting in a coarsening of the fiber diameter under the same electric field conditions. The result of comparative example 2 is that the stretching and splitting of the jet is not favored because the electric field strength is too great due to the too high applied voltage.

Test example 2

The fiber diameter distribution of the silk fibroin peptide nanofiber membrane prepared in example 3 is shown in fig. 6. As can be seen from fig. 6, the fiber diameter is mainly distributed between 0.2 μm and 0.4 μm, and has a larger specific surface area and a higher pore structure, and can load more active substances.

Test example 3

After spraying a proper amount of water on the skin, the silk fibroin peptide nanofiber facial mask prepared in example 3 was applied to the surface, and after 8 seconds, as shown in fig. 7. As can be seen from fig. 7, the silk fibroin peptide nanofiber mask was completely absorbed and dissolved. The skin intelligent instrument detects the moisture content of the skin after application, the moisture content reaches 45%, and compared with the normal skin moisture content, the moisture content is obviously improved by about 50% -80%, and the skin absorbs partial moisture and active substances, so that the effect of rapid and efficient moisture preservation can be realized.

It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations and modifications of the present invention will be apparent to those of ordinary skill in the art in light of the foregoing description. It is not necessary here nor is it exhaustive of all embodiments. And obvious variations or modifications thereof are contemplated as falling within the scope of the present invention.

Claims

1. A silk fibroin peptide nanofiber facial mask, characterized in that the raw materials of the silk fibroin peptide nanofiber facial mask, measured by weight, include 10%-15% of water-soluble polymers, 0.5%-3% of silk fibroin peptides, 0.5%-3% of active substances, 3%-5% of polysaccharides, 2%-5% of thickening substances, and the balance is water; the silk fibroin peptide nanofiber facial mask is prepared by double-needle electrospinning technology.

2. The silk fibroin peptide nanofiber facial mask according to claim 1, characterized in that the water-soluble polymer is selected from one or more of hydrogenated starch, hydroxymethyl cellulose, ethylene oxide, polyvinyl pyrrolidone and polyvinyl alcohol.

3. The silk fibroin peptide nanofiber mask according to claim 1, characterized in that the active substance is selected from one or more of sodium hyaluronate, niacinamide, retinol and their derivatives.

4. The silk fibroin peptide nanofiber facial mask according to claim 1, characterized in that the molecular weight of the silk fibroin peptide is 500Da-2000Da.

5. The silk fibroin peptide nanofiber facial mask according to claim 1, characterized in that the polysaccharide substance is selected from one or more of sodium alginate, chitosan and mannose.

6. The silk fibroin peptide nanofiber facial mask according to claim 1, characterized in that the thickening substance is selected from one or more of gum arabic, gelatin and xanthan gum.

7. A method for preparing the silk fibroin peptide nanofiber mask according to any one of claims 1 to 6, characterized in that it comprises the following steps:

S1, dissolving a water-soluble polymer, silk protein peptide, active substance, polysaccharide substance and thickening substance in water, stirring evenly, standing to defoam to obtain a spinning solution;

S2. The spinning solution described in S1 is made into the silk fibroin peptide nanofiber mask through double-needle electrospinning technology.

8. The method for preparing a silk fibroin peptide nanofiber mask according to claim 7 is characterized in that, in S1, the dissolution temperature of the water-soluble polymer is 70°C-80°C, and the stirring time is 2h-4h; the dissolution temperature of the silk fibroin peptide, active substance, polysaccharide substance and thickening substance is 70°C-80°C, and the stirring time is independently 4h-6h.

9. The method for preparing a silk fibroin peptide nanofiber facial mask according to claim 7, characterized in that, in S1, the standing degassing time is 5h-8h.

10. The method for preparing a silk fibroin peptide nanofiber mask according to claim 7 is characterized in that, in S2, the process conditions of the double-needle electrospinning are: the first voltage is 10kV-20kV, the second voltage is 15kV-25kV, the injection rate is 1mL/h-2mL/h, the temperature is 15℃-30℃, and the humidity is 20%-30%.