CN120501112B - Mite-killing composition and application thereof - Google Patents
Mite-killing composition and application thereofInfo
- Publication number
- CN120501112B CN120501112B CN202510628888.1A CN202510628888A CN120501112B CN 120501112 B CN120501112 B CN 120501112B CN 202510628888 A CN202510628888 A CN 202510628888A CN 120501112 B CN120501112 B CN 120501112B
- Authority
- CN
- China
- Prior art keywords
- active ingredient
- formula
- agent
- acaricidal composition
- test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/14—Ethers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/713—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with four or more nitrogen atoms as the only ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/02—Acaricides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticide mite killing, and discloses a mite killing composition and application thereof, wherein the mite killing composition comprises an active ingredient A and an active ingredient B, and the active ingredient A is a compound shown in a formula I:
Description
The invention relates to a mite-killing composition and application thereof, wherein the application number is 202311021235.4, the application date is 2023, 8 and 15, and the application name is a divisional application of 'mite-killing composition and application thereof'.
Technical Field
The invention belongs to the technical field of pesticide mite killing, and discloses a mite killing composition and application thereof.
Background
Mites are arachnids pests, and are one of important pests of various crops in China. The individual of the pests is tiny, quick in propagation, quick in variation and easy to produce
Resistance is generated, and chemical control is very difficult.
The chemical control and biological control are combined, so that the harm of mites can be effectively controlled, and the dosage of pesticides is reduced, thereby coordinating
The relationship between biological control and chemical control reduces the influence of pesticides on the environment, natural enemies and the drug resistance of pests, and it is important to find chemical pesticides which are efficient on target pests and have small influence on natural enemies.
Therefore, the applicant selects any one of the compound shown in the formula I and the hexythiazox or clofentezine for reasonable compounding and use, determines that the compound has synergistic effect on target mites, and is safe and optimal in proportion to natural enemy beneficial insects, the dosage of the medicament is reduced, and the natural enemy of the mites is protected to the greatest extent
The mite killing agent is provided with a basis for reasonably using the mite killing agent, so that the safe use of the agent is realized, and the influence of pesticides on the environment and natural enemies is reduced.
Disclosure of Invention
Based on the problems in the prior art, the invention provides a mite-killing composition which has better synergistic effect on common plant mites, basically has no poison on natural enemy beneficial insects, reduces the dosage of medicaments, prevents and controls the mites, and simultaneously furthest protects the mites
The preparation prepared from the miticidal composition has the advantages of good quick-acting property, long lasting period and environmental pollution reduction.
In order to achieve the aim, the invention adopts the following technical scheme that the acaricidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is a compound shown in a formula I:
the active ingredient B is selected from any one of hexythiazox or clofentezine;
further, the mass ratio of the active ingredient A to the active ingredient B is 1:50-50:1;
Further, the active ingredient B is hexythiazox, and the mass ratio of the active ingredient A to the active ingredient B is 1:45-45:1;
the active ingredient B is clofentezine, and the mass ratio of the active ingredient A to the active ingredient B is 1:42-36:1.
Further, the active ingredient B is hexythiazox, and the mass ratio of the active ingredient A to the active ingredient B is 1:22-35:1;
the active ingredient B is clofentezine, and the mass ratio of the active ingredient A to the active ingredient B is 1:20-30:1;
further, the acaricidal composition also comprises auxiliary components, wherein the auxiliary components are carriers or auxiliary agents;
Further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl sodium succinate, alpha-olefin sulfonate, alkylphenol ethoxylate, castor oil ethoxylate, alkylphenol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm excrement, chinese honeylocust fruit powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrating agent F, and/or
The dispersant is selected from one or more of lignosulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, fatty amine polyoxyethylene ether, glycerin fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylic acid, phosphate, EO-PO block copolymer and EO-PO graft copolymer, and/or
The thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate, and carboxymethyl cellulose, and/or
The disintegrating agent is one or more selected from sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid, and/or
The emulsifier is one or more selected from fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenethyl phenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrylphenol polyoxyethylene ether and fatty acid polyoxyethylene ester, and/or
The defoamer is one or more selected from silicone oil, C 10～C20 saturated fatty acid compound, C 8～C10 fatty alcohol compound or silicone compound, and/or
The preservative is one or more selected from sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, and/or
The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2, 6-di-tert-butyl-p-cresol, and epoxidized vegetable oil, and/or
The synergistic agent is selected from synergistic phosphorus, synergistic ether, and/or
The carrier is one or more selected from kaolin, bentonite, attapulgite, light calcium carbonate, diatomite and white carbon black, and/or
The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, N-butanol, diesel oil, N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil derivatives and deionized water, and/or
The above auxiliary components are all commercially available.
Further, the total weight of the active ingredient A and the active ingredient B accounts for 0.5% -80% of the total weight of the acaricidal composition;
further, the acaricidal composition can be prepared into a liquid preparation or a solid preparation, wherein the liquid preparation is selected from soluble agents, oil agents, spreading oil agents, emulsifiable concentrates, emulsion, dispersible agents, paste, aqueous emulsion, oil emulsion, microemulsion, lipid suspending agents, microcapsule suspending agents, oil suspending agents, dispersible oil suspending agents, suspending emulsion, microcapsule suspension-suspending agents or microcapsule suspension-suspending agents, and the solid preparation is selected from powder, granule, ball, tablet, strip, wettable powder, oil dispersion powder, emulsion powder, water dispersible granule, emulsion granule, water dispersible tablet, soluble powder, soluble tablet or soluble granule.
Further, the preparation formulation is suspending agent, missible oil, microemulsion, aqueous emulsion, water dispersible granule or wettable powder.
The invention also discloses the use of the acaricidal composition for preventing and controlling plant pest mites.
Further, the plant is an agricultural crop or a forestry crop;
further, the plant pest mites are tetranychidae, echinococcidae and/or dermatophagoides pteronyssinus;
Further, the Tetranychus urticae is Tetranychus urticae, tetranychus cinnabarinus, tetranychus truncatum, tetranychus urticae, tetranychus citri and/or Tetranychus urticae, wherein the Tetranychus urticae is Alternaria citri, alternaria aegypti and/or Alternaria sinensis, and the Tetranychus urticae is Tetranychus urticae.
The acaricidal composition disclosed by the invention has the beneficial effects that acaricides with different action mechanisms are reasonably compounded, the acaricidal composition has a good synergistic effect on common mites, is safe to natural enemies, reduces the dosage of the medicament, and is good in quick-acting performance and long in lasting period.
Detailed Description
The present invention will be described in more detail with reference to the following examples, but the present invention can be embodied in various forms and should not be construed as being limited to the embodiments set forth herein.
Preparation example:
Preparation 1:14% Compound of formula I, hexythiazox aqueous emulsion (1:6)
The formula comprises 2% of a compound of formula I, 12% of hexythiazox, 1% of Guerbet alcohol polyoxyethylene ether, 6% of alkylaryl polyoxyethylene ether polyoxypropylene ether, 8% of cyclohexanone, 0.2% of xanthan gum, 5% of ethylene glycol, 0.1% of sodium benzoate, 0.1% of silicone oil and deionized water, and the balance of the components;
The preparation method comprises the steps of dissolving active ingredients in a solvent according to the formula proportion of a preparation example, adding an emulsifier, dissolving into a uniform oil phase, mixing deionized water and an antifreezing agent together to form a uniform water phase, and adding the water phase into the oil phase under high-speed shearing to form a well-dispersed aqueous emulsion product.
PREPARATION EXAMPLE 25% Compound of formula I Thioxazin microemulsion (4:1)
The formula comprises 4% of a compound of formula I, 1% of hexythiazox, 1% of alkylphenol polyoxyethylene ether phosphate, 10% of styrylphenol polyoxyethylene ether, 2% of fatty alcohol polyoxyethylene ether sodium sulfate, 2% of calcium dodecyl benzene sulfonate, 5% of alkyl glycoside, 2% of xylene, 7% of cyclohexanone, 3% of ethylene glycol, 0.1% of kathon, 0.5% of glycerol, 0.1% of xanthan gum and deionized water, wherein the balance is the balance;
the preparation method comprises the steps of uniformly mixing active ingredients, a solvent, an emulsifier and the like according to the formula proportion of a preparation example to prepare an oil phase, uniformly mixing an antifreezing agent with water to prepare a water phase, adding the oil phase into the water phase under a stirring state, uniformly stirring, continuously shearing for 10min, adding silicone oil, a defoaming agent, xanthan gum and the like, and uniformly stirring to obtain small liquid drops with the oil phase particles of 0.01-0.1 micrometer, thus preparing the microemulsion.
Preparation example 3:9% of Compound of formula I, hexythiazox emulsifiable concentrate (8:1)
8% Of a compound of formula I, 1% of hexythiazox, 10% of DMF, 8% of alkylphenol ethoxylates, 2% of calcium dodecyl benzene sulfonate, 15% of propylene carbonate and the balance of dimethylbenzene;
the preparation method comprises the steps of adding the metered active ingredients, the solvent and the cosolvent into a blending kettle, stirring to dissolve the active ingredients, adding the emulsifier, supplementing the rest with the rest solvent, uniformly stirring in the stirring kettle, and filtering to obtain the emulsifiable concentrate required by the invention.
Preparation example 4. 22% Compound of formula I, hexythiazox wettable powder (10:1)
The formula comprises 20% of a compound of formula I, 2% of hexythiazox, 3% of sodium polycarboxylate, 8% of naphthalene sulfonate formaldehyde condensate, 2% of nekal BX, 5% of white carbon black and the balance of kaolin;
The preparation method comprises the steps of mixing the effective components, the dispersing agent, the wetting agent and the filler according to the formula proportion, uniformly stirring in a stirring kettle, and carrying out multiple crushing and uniform mixing through a jet mill to obtain the wettable powder of the composition.
PREPARATION EXAMPLE 5 18% Compound of formula I Thioxazin suspension (5:1)
15% Of a compound shown in a formula I, 3% of hexythiazox, 2% of isomeric tridecanol polyoxyethylene ether, 4% of styrol polyoxyethylene ether phosphate, 1% of sodium lignin sulfonate, 0.25% of xanthan gum, 3% of ethylene glycol, 0.5% of kathon, 0.5% of silicone oil and the balance of deionized water;
the preparation method comprises the steps of sequentially placing active ingredients, a surfactant and other functional additives in a reaction kettle according to the formula proportion, adding water, uniformly mixing, shearing at a high speed, performing wet sanding, and finally homogenizing and filtering to obtain a suspending agent product.
Preparation example 6:18% Compound of formula I clofentezine suspension agent (1:8)
The formula comprises 2% of compound of formula I, 16% of clofentezine, 1% of alkylaryl polyoxyethylene ether polyoxypropylene ether, 1% of sodium lignin sulfonate, 3% of styrol polyoxyethylene ether sulfate, 2% of fatty alcohol polyoxyethylene ether, 0.5% of magnesium aluminum silicate, 0.2% of xanthan gum, 5% of ethylene glycol, 0.2% of potassium benzoate, 0.5% of silicone oil and deionized water, and the balance is filled;
the preparation method is the same as that of preparation example 5.
Preparation example 7:18% Compound of formula I clofentezine wettable powder (8:1)
The formula comprises 16% of a compound of a formula I, 2% of clofentezine, 4% of sodium polycarboxylate, 8% of sodium lignin sulfonate, 2% of fenpropargyl BX, 5% of white carbon black and the balance of kaolin;
the preparation method is the same as that of preparation example 4.
Preparation example 8:33% Compound of formula I clofentezine Water dispersible granule (10:1)
The formula comprises 30% of a compound shown in a formula I, 3% of clofentezine, 1% of succinate sulfonate, 10% of naphthalene sulfonate formaldehyde condensate, 5% of sodium polycarboxylate, 1% of sodium dodecyl sulfate, 5% of white carbon black, 30% of starch and the balance of kaolin.
The preparation method comprises the steps of adding active ingredients into a carrier according to the formula proportion of the embodiment, adding a surfactant and other functional additives into the carrier, mixing, adding 10-25% of water after jet milling, and then kneading, granulating, drying and screening to obtain a water dispersible granule product, or spraying water, granulating, drying and screening the crushed powder in a boiling granulator to obtain the product.
Indoor Activity test
Example 1 Compound of formula I test of indoor Activity of Tetranychus urticae
The test was carried out with reference to NY/T1154.12-2008 "pesticide laboratory biological assay test criterion pesticide part 12: tetranychus slide dipping method".
Biological test material, tetranychus urticae.
Test material preparation, namely selecting the mites with consistent indoor feeding and physiological states. Cutting double-sided adhesive tape into 2cm long, attaching to one end of a glass slide, selecting healthy mites, adhering the back of each piece of the double-sided adhesive tape to 30 heads of the double-sided adhesive tape, placing the double-sided adhesive tape into an insect-raising box filled with wet sponge, covering a cover, placing the double-sided adhesive tape under the condition of (25+/-1) DEG C, performing microscopic examination after 2 hours, removing dead individuals and injured individuals, and supplementing each piece of the double-sided adhesive tape with 30 heads.
The preparation method comprises dissolving the compound original drug, clofentezine original drug and hexythiazox original drug in the test formula I with acetone to obtain mother solution, and preparing 5 series of mass concentrations with 0.1% Tween-80 aqueous solution according to an equal ratio method.
And (3) medicament treatment, namely immersing the glass slide in the medicament, slightly shaking for 5 seconds, taking out, sucking the excessive medicament liquid by using water absorbing paper, and placing the glass slide in a white porcelain plate filled with wet sponge to cover the glass slide by using a plastic film with good light transmittance.
Each treatment was repeated 4 times and a treatment without agent (with all organic solvents and emulsifiers) was set as a blank.
Feeding and observing, namely feeding and observing the container with the treated test insects under proper conditions.
Checking, namely checking the death condition of the test insects 48 hours after treatment, and respectively recording the total number of insects and the death number of the test insects.
Data statistics and analysis:
and calculating the corrected mortality of each treatment according to the investigated data, and calculating according to the following formula, wherein the calculation results are reserved to the two decimal places.
Wherein:
P-mortality in percent (%);
K-represents the number of dead insects in units of heads;
n-represents the total number of insects treated in units of heads.
Wherein:
P 1 -correct mortality in percent (%);
P t -mortality in percent (%);
P 0 -control mortality in percent (%).
If the control mortality rate is less than 5% and no correction is needed, the control mortality rate is between 5% and 20%, the correction is carried out according to a correction mortality rate formula, the control mortality rate is more than 20%, and the test is reworked.
The LC 50 value was obtained by analysis using DPS statistical analysis system, and the activity of the test agent on the biological test material was evaluated.
The co-toxicity coefficient (CTC value) of the blend is calculated as follows:
wherein:
ati—actual measured virulence index of the mixture;
S-LC 50 of standard acaricide in milligrams per liter (mg/L);
M-LC 50 of the mix in milligrams per liter (mg/L).
TTI=TIA×PA+TIB×PB
Wherein:
TTI-the theoretical toxicity index of the mixture;
TI A A agent toxicity index;
the percentage content of the P A -A medicament in the mixture is expressed as percentage (%);
TI B -agent toxicity index;
The percentage of the P B -B medicament in the mixture is expressed as percentage (%).
Wherein:
Ctc—co-toxicity coefficient;
ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
The compounded co-toxicity coefficient CTC is more than or equal to 120 and shows synergistic effect, CTC is less than or equal to 80 and shows antagonistic effect, and CTC 80 is less than or equal to 120 and shows additive effect. The results of the indoor test are shown in the following table:
table 1 Compound of formula I and hexythiazox type test of indoor biological Activity against Tetranychus urticae
| Test agent | LC50(mg/L) | (ATI) | (TTI) | Co-toxicity coefficient (CTC) |
| Compounds of formula I (A) | 2.5833 | 100.000 | / | / |
| Hexythiazox (B 1) | 11.4095 | 22.642 | / | / |
| A:B1=1:45 | 10.0802 | 25.627 | 24.323 | 105.362 |
| A:B1=1:22 | 8.0102 | 32.250 | 26.005 | 124.015 |
| A:B1=1:15 | 6.9503 | 37.168 | 27.477 | 135.272 |
| A:B1=1:8 | 5.1082 | 50.572 | 31.237 | 161.896 |
| A:B1=4:1 | 1.4992 | 172.312 | 84.528 | 203.851 |
| A:B1=8:1 | 1.4924 | 173.097 | 91.405 | 189.374 |
| A:B1=12:1 | 1.6093 | 160.523 | 94.049 | 170.680 |
| A:B1=20:1 | 1.8227 | 141.729 | 96.316 | 147.150 |
| A:B1=25:1 | 1.9812 | 130.391 | 97.025 | 134.389 |
| A:B1=35:1 | 2.1049 | 122.728 | 97.851 | 125.423 |
Table 2 indoor biological Activity test of Compound of formula I and clofentezine on Tetranychus urticae
| Test agent | LC50(mg/L) | (ATI) | (TTI) | Co-toxicity coefficient (CTC) |
| Compounds of formula I (A) | 2.5833 | 100.000 | / | / |
| Clofentezine (B 2) | 6.0274 | 42.859 | / | / |
| A:B2=1:42 | 4.6004 | 56.154 | 44.188 | 127.079 |
| A:B2=1:21 | 3.9406 | 65.556 | 45.457 | 144.217 |
| A:B2=1:11 | 3.4753 | 74.333 | 47.621 | 156.093 |
| A:B2=1:5 | 2.4377 | 105.973 | 52.383 | 202.305 |
| A:B2=4:3 | 1.5097 | 171.113 | 75.511 | 226.607 |
| A:B2=7:1 | 1.5554 | 166.086 | 92.857 | 178.861 |
| A:B2=10:1 | 1.6932 | 152.569 | 94.805 | 160.929 |
| A:B2=15:1 | 1.8856 | 137.001 | 96.429 | 142.075 |
| A:B2=22:1 | 2.0573 | 125.567 | 97.516 | 128.767 |
| A:B2=36:1 | 2.1081 | 122.542 | 98.456 | 124.464 |
As can be seen from tables 1 and 2, the compound of the formula I, clofentezine and hexythiazox show higher toxicity to the two-spotted spider mites, and the compound of the formula I has the best control effect on the two-spotted spider mites. The compound of the formula I and the hexythiazox do not have antagonism to the tetranychus urticae in the range of 1:45-35:1, wherein the co-toxicity coefficient of the compound of the formula I and the hexythiazox is greater than 120 in the range of 1:22-25:1, and the co-toxicity coefficient of the compound of the formula I and the tetranychus methyl in the range of 1:42-36:1 is greater than 120, and the synergistic effect is shown.
Example 2 safety test of Compound of formula I on Natural enemy, isoaybug
Test target is 3-year larva of the ladybug.
Test agent the original organic solvent was prepared as mother liquor and dissolved in 0.1% tween-80 aqueous solution, the agent was prepared according to the optimum ratio of the compound of formula I in example 1 to clofentezine or hexythiazox, and the treatment without agent (containing all organic solvents and emulsifiers) was set as blank control.
The preparation of test materials comprises selecting clean cotton leaf which grows consistently and is not applied with the agent, cutting into 6cm diameter leaf discs, and spreading in plastic cup containing agar. 200 heads of indoor-bred spider mites are inoculated on leaf discs, and 1 head of the heteroladybug larvae starved for 4 hours are inoculated into each cup.
And (3) medicament treatment, namely placing the plastic cup into a potter spray tower for spray treatment, taking out after the liquid medicament is settled for 1min, and sealing the top of the cup by using a gauze with the aperture of 0.15mm so as to prevent test insects from escaping.
Feeding and observing, namely feeding and observing the treated test insects under the conditions of temperature (25+/-1) DEGC, relative humidity (70+/-5)%, photoperiod L: D= (16:8) h, checking the death condition of the heteroladybug after 48h and recording the death quantity, wherein the dead of the brush pen light touch insects is regarded as death.
Each agent concentration and control was replicated 4 times, each replicate tested for 30 head 3-year-old heteroladybug larvae.
Data statistics and analysis:
and calculating the corrected mortality of each treatment according to the investigated data, and calculating according to the following formula, wherein the calculation results are reserved to the two decimal places.
Wherein:
P-mortality in percent (%);
K-represents the number of dead insects in units of heads;
n-represents the total number of insects treated in units of heads.
Wherein:
P 1 -correct mortality in percent (%);
P t -mortality in percent (%);
P 0 -control mortality in percent (%).
If the control mortality rate is less than 5% and no correction is needed, the control mortality rate is between 5% and 20%, the correction is carried out according to a correction mortality rate formula, the control mortality rate is more than 20%, and the test is reworked.
And (3) analyzing by using a DPS statistical analysis system, solving a virulence regression equation and an LC 50 value, and evaluating the activity of the test agent on the biological test material.
The safety coefficient is adopted to evaluate the safety degree of various medicaments on the heteroladybug:
when the toxicity selection index STR is less than 1, the chemical agent has negative selectivity to natural enemies and pests (mites), when STR=1, the chemical agent has no obvious selectivity to the natural enemies and the pests (mites), when 1< STR is less than or equal to 10, the chemical agent has positive selectivity to the natural enemies and the pests (mites), when 10< STR is less than or equal to 100, the chemical agent has moderate positive selectivity to the natural enemies and the pests, when 100< STR is less than or equal to 1000, the chemical agent has high positive selectivity to the natural enemies and the pests (mites), and when STR is more than 1000, the chemical agent has strong positive selectivity to the natural enemies and the pests (mites).
TABLE 3 safety test results of Compound of formula I on natural enemy, isoaybug, with clofentezine or hexythiazox
As can be seen from Table 3, the toxicity of the compound of formula I to the heteroladybug by compounding with clofentezine or hexythiazox is obviously lower than that of the heteroladybug to mites, and the heteroladybug is relatively safe to natural enemy insects.
Field efficacy test
Example 3 field efficacy test of acaricidal composition on Cotton Red spider
The cotton field Yuan Zhuangcun is paved in thirty-county high in Liaocheng city in Shandong province, the soil fertility is medium, and the cultivation conditions of all the test communities are uniform and consistent with the local agricultural cultivation measures.
And the test target is cotton red spider.
The test method is that the medicine is applied for 1 time at the initial stage of the occurrence of the cotton red spider, and quantitative spraying is carried out by a knapsack sprayer during the medicine application, and no other medicines for preventing and treating diseases and insect pests are used during the test.
Test investigation, wherein investigation is carried out 3d, 7d and 14d after the drug administration, 20 leaves are fixed in each district for 3 times of investigation, the number of live dynamic mites is counted, and the mite mouth reduction rate and the prevention and treatment effect are calculated.
Safety investigation each trial investigation was accompanied by an observation of whether each treatment agent had an abnormal effect on cotton growth and a negative effect on non-target organisms.
And (3) carrying out statistics on test data:
the test results are shown in the following table:
Table 4 results of field efficacy test of the acaricidal composition on Cotton Red spider
The field efficacy test results show that the acaricidal composition can effectively prevent and treat the harm of cotton red spiders, and each compound preparation has good quick-acting property and long lasting period. Whether each treatment agent had an abnormal effect on cotton growth or not and whether there was a detrimental effect on non-target organisms was not found throughout the test.
Example 4 field efficacy test of acaricidal composition on Tetranychus urticae
The test is set in a small Liu Cun apple garden in the suburb of Zhengzhou, henan province, the variety is vine wood, 15 years old, the plant-row spacing is 2m multiplied by 4m, the height of the tree is about 2.5m, the apple leaf mites are serious in successive years, and the type of the mites is mainly hawthorn leaf mites through field investigation and indoor microscopic examination. The test was administered on day 22 of 5 months 2020 (weather, temperature 21 ℃ C./35 ℃ C.).
Test and control agents are shown in the following table (table 5).
The application method is that the conventional application is adopted in the test, and the backpack electric sprayer is adopted to uniformly apply the pesticide to the whole plant of the fruit tree, and the front and the back of the leaf blade are sprayed.
Test design, the area of the test plot is 2 fruit trees, and each treatment is repeated 4 times.
The test is carried out according to GB/T17980.7-2000, the mite base number is investigated before administration, and the number of active mites is investigated 3d, 7d and 14d after administration. When the method is used for investigation, the number of the active mites on 5 leaves of each tree in each district is randomly investigated according to 5 directions of east, south, west, north and middle, the leaves are investigated by using a handheld magnifying glass, and the number of the active mites is recorded.
And (3) carrying out statistics on test data:
the test results are shown in the following table:
table 5 results of field efficacy test of mite-killing composition on Tetranychus urticae
As can be seen from an indoor toxicity test and a field efficacy test, the acaricidal composition provided by the invention has a good control effect on mites and is safe to natural enemy insects.
While the invention has been described in detail in terms of the general description and the specific embodiments, it will be apparent to those skilled in the art that various modifications and improvements can be made thereto without departing from the spirit of the invention.
Claims (7)
1. The acaricidal composition is characterized by comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is a compound shown in a formula I: (formula I),
The active ingredient B is clofentezine, and the mass ratio of the active ingredient A to the active ingredient B is 1:42-36:1.
2. The acaricidal composition according to claim 1, wherein the mass ratio of the active ingredient a to the active ingredient B is 1:20-30:1.
3. The acaricidal composition according to claim 1, further comprising an auxiliary ingredient, wherein the auxiliary ingredient is a carrier or an adjuvant.
4. The acaricidal composition according to claim 1, wherein the total weight of the active ingredient a and the active ingredient B is 0.5% -80% of the total weight of the acaricidal composition.
5. The acaricidal composition according to claim 1, wherein the acaricidal composition is prepared into a liquid preparation or a solid preparation, the liquid preparation is selected from the group consisting of a soluble agent, a spreading oil agent, an emulsifiable concentrate, a latex, a dispersible agent, a paste, an aqueous emulsion, an oil emulsion, a microemulsion, a lipid suspending agent, a microcapsule suspending agent, a dispersible oil suspending agent, a suspoemulsion, a microcapsule suspension-suspending agent, a microcapsule suspension-aqueous emulsion or a microcapsule suspension-suspending agent, and the solid preparation is selected from the group consisting of a sphere, a strip, a wettable powder, an oil-dispersible powder, a emulsion powder, a water dispersible granule, a milk granule, a water dispersible tablet, a soluble powder, a soluble tablet or a soluble granule.
6. The acaricidal composition according to claim 5, wherein the formulation is a suspension, an emulsifiable concentrate, a microemulsion, an aqueous emulsion, a dispersible oil suspension, a water dispersible granule or a wettable powder.
7. Use of the acaricidal composition according to any one of claims 1 to 6 for the prevention and control of plant pest mites, characterised in that said pest mites are spider mites.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202510628888.1A CN120501112B (en) | 2023-08-15 | 2023-08-15 | Mite-killing composition and application thereof |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311021235.4A CN117063928B (en) | 2023-08-15 | 2023-08-15 | Mite-killing composition and application thereof |
| CN202510628888.1A CN120501112B (en) | 2023-08-15 | 2023-08-15 | Mite-killing composition and application thereof |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311021235.4A Division CN117063928B (en) | 2023-08-15 | 2023-08-15 | Mite-killing composition and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN120501112A CN120501112A (en) | 2025-08-19 |
| CN120501112B true CN120501112B (en) | 2026-04-17 |
Family
ID=88718821
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311021235.4A Active CN117063928B (en) | 2023-08-15 | 2023-08-15 | Mite-killing composition and application thereof |
| CN202510628888.1A Active CN120501112B (en) | 2023-08-15 | 2023-08-15 | Mite-killing composition and application thereof |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311021235.4A Active CN117063928B (en) | 2023-08-15 | 2023-08-15 | Mite-killing composition and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN117063928B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN120021623B (en) * | 2023-11-23 | 2026-02-17 | 海利尔药业集团股份有限公司 | Mite-killing composition and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110037021A (en) * | 2019-05-22 | 2019-07-23 | 侨昌现代农业有限公司 | A kind of acaricidal miticide composition of Synergistic type of class containing biphenyl |
| CN113943238A (en) * | 2020-07-17 | 2022-01-18 | 沈阳中化农药化工研发有限公司 | Substituted diphenyl sulfide compound and application thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69934224T2 (en) * | 1998-04-27 | 2007-10-04 | Kumiai Chemical Industry Co., Ltd. | 3-ARYLPHENYLSULFIDE DERIVATIVES AND INSECTICIDES AND MITICIDES |
| JP4950894B2 (en) * | 2005-09-21 | 2012-06-13 | クミアイ化学工業株式会社 | Biphenyl sulfide compounds and insecticide / acaricide |
| JP2009023910A (en) * | 2007-07-17 | 2009-02-05 | Kumiai Chem Ind Co Ltd | Biphenyl sulfide compounds and insecticide / acaricide |
| CN114957062B (en) * | 2022-07-05 | 2023-12-29 | 海利尔药业集团股份有限公司 | Para-substituted biphenyl thioether compound, composition and application thereof |
-
2023
- 2023-08-15 CN CN202311021235.4A patent/CN117063928B/en active Active
- 2023-08-15 CN CN202510628888.1A patent/CN120501112B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110037021A (en) * | 2019-05-22 | 2019-07-23 | 侨昌现代农业有限公司 | A kind of acaricidal miticide composition of Synergistic type of class containing biphenyl |
| CN113943238A (en) * | 2020-07-17 | 2022-01-18 | 沈阳中化农药化工研发有限公司 | Substituted diphenyl sulfide compound and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117063928A (en) | 2023-11-17 |
| CN120501112A (en) | 2025-08-19 |
| CN117063928B (en) | 2025-10-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101669492A (en) | Synergic agricultural pesticide composition | |
| CN120501112B (en) | Mite-killing composition and application thereof | |
| CN114916555B (en) | Application of pesticide composition containing bistrifluron in controlling plant pest mites | |
| CN117770263A (en) | Mite-killing composition and application thereof | |
| CN118020769B (en) | A mite-killing composition and its application | |
| CN117958268A (en) | Mite-killing composition and application thereof | |
| CN116491512A (en) | Nematicidal composition containing cyclob rifluram and application thereof | |
| CN118830545B (en) | A mite-killing composition and its application | |
| CN119138424B (en) | A miticide composition containing propargite and its application | |
| CN117598317B (en) | Pesticide composition and application thereof | |
| CN116369327B (en) | Mite-killing composition containing hexythiazox and application thereof | |
| CN118216501B (en) | A pesticide composition containing profenofos and its application | |
| CN117770267B (en) | Mite-killing composition and application thereof | |
| CN114586789B (en) | Mite-killing composition and application thereof | |
| CN119791115B (en) | A pesticide composition and its application | |
| CN120130478B (en) | A pesticide composition and its use | |
| CN120113669B (en) | Acaricide and its application | |
| CN119949325B (en) | A pesticide composition containing flufenoxuron and its application | |
| CN119699344B (en) | A pesticide composition and its application | |
| CN119999688B (en) | A mite-killing composition and its application | |
| CN119234816B (en) | Insecticide and acaricide composition containing tetronic acid compounds and its application | |
| CN118661737B (en) | A mite-killing composition | |
| CN121867224A (en) | A mite-killing composition and its application | |
| CN121621357A (en) | Mite-killing composition and application thereof | |
| CN117397693A (en) | Acaricide and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |