CN1255191C - Stem cell cardiac muscle steoro transplanter - Google Patents
Stem cell cardiac muscle steoro transplanter Download PDFInfo
- Publication number
- CN1255191C CN1255191C CN 200310121735 CN200310121735A CN1255191C CN 1255191 C CN1255191 C CN 1255191C CN 200310121735 CN200310121735 CN 200310121735 CN 200310121735 A CN200310121735 A CN 200310121735A CN 1255191 C CN1255191 C CN 1255191C
- Authority
- CN
- China
- Prior art keywords
- stem cell
- cardiac muscle
- syringe
- syringe needle
- needle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000004165 myocardium Anatomy 0.000 title claims abstract description 35
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 35
- 239000000725 suspension Substances 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 10
- 230000000302 ischemic effect Effects 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 3
- 239000006285 cell suspension Substances 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 108010080379 Fibrin Tissue Adhesive Proteins 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 abstract description 8
- 230000004217 heart function Effects 0.000 abstract description 4
- 206010012559 Developmental delay Diseases 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 3
- 206010058314 Dysplasia Diseases 0.000 description 2
- 230000000747 cardiac effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003387 muscular Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 238000010999 medical injection Methods 0.000 description 1
- 208000031225 myocardial ischemia Diseases 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000002629 repopulating effect Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
The present invention discloses a stem cell cardiac muscle stereo planter which is composed of a needle head, a needle holder, a flexible pipe and a syringe, wherein a suspension groove is arranged in the needle holder, and the combined needle head is inserted in the suspension groove from down to top; a joint of the flexible pipe is connected with an outlet end of the syringe, and the other end of the flexible pipe is connected with the needle holder and is inserted into the suspension groove. The stem cell cardiac muscle stereo planter specially used for planting stem cells of ischemic cardiac muscle can carry out stereo planting for the stem cells in the outer layer, the middle layer and the inner layer of the cardiac muscle simultaneously, avoid the stem cells from developmental disturbance in the cardiac muscle due to the uneven distribution of cells in the traditional stem cell planting process and preferably improve heart functions. The present invention also discloses a preparation process and a use method of the stem cell cardiac muscle stereo planter.
Description
Technical field
The present invention relates to a kind of medical injection apparatus.
Background technology
The method of present plantation stem cell has multiple: coronary perfusion method, intracardiac intervention injection, the interior injecting method of the direct cardiac muscle of ordinary syringe etc.The shortcoming that can not make all myocardial ischemia positions plant a considerable amount of cells and cardiac muscle all be had the secondary damage effect is all arranged in these methods.
Summary of the invention
The purpose of this invention is to provide a kind of stem cell cardiac muscle stereo planting device, can carry out stereo planting, avoid in the conventional dry cell seeding process improving cardiac function better because the cell distribution inequality causes stem cell dysplasia in cardiac muscle to stem cell.
For achieving the above object, the present invention takes following scheme:
Constitute by combination syringe needle, needle stand, flexible pipe and syringe; One suspension groove is arranged, in the bottom-up insertion suspension of the combination syringe needle groove in needle stand; The joint of flexible pipe and the port of export of syringe join, and the other end and the needle stand of flexible pipe join, and are inserted in the suspension groove.
Combination syringe needle part: be injected directly in the cardiac muscular tissue, short, in, long three degree of depth enter skin, middle level, and the internal layer of cardiac muscle respectively.The head end of syringe needle becomes blunt round, and the length on syringe needle slope is less than 1mm, is the damage that causes for the cutting that reduces cardiac muscle as far as possible.Distance among every row between adjacent two syringe needles should be equal substantially, and this is even for repopulating cell, and the cell quantity of every bit is unlikely also very little unlikely too much.Syringe needle quantity can be 9 or 16.In order to be expelled to cardiac muscular tissue exactly, the length of syringe needle being stretched out the needle stand lower surface has qualification clearly.If 9 syringe needles then can be by 2 3.5mm syringe needles, 3 4.5mm syringe needles and 4 6.5mm syringe needles; Or 2 3.0mm syringe needles, 3 2.0mm syringe needles and 4 5.0mm syringe needles.
Needle stand: be used for fixing syringe needle, the even vertical distribution of each syringe needle is on needle stand.Needle stand is that medical plastic is made, and tissue is not had toxic and side effects.
The syringe of band bayonet socket piston: be the container of stem cell suspension, make by common syringe, 2～10 equidistant bayonet sockets are arranged on the piston of this syringe, bayonet socket can be stuck on the baffle plate of syringe end, when the piston of syringe when the clockwise direction rotation is advanced, revolve whenever that syringe can spray 0.5ml cell suspension when adjacent two bayonet sockets.
Preparation method of the present invention can be to choose length 1 * 1 * 0.8cm
3Medical grade plastic materials is made a call to 9 or 16 holes with laser along the vertical planar direction of needle stand from the lower surface of needle stand, the syringe needle 9 that grinds or 16 are installed, and becomes above-mentioned shape.
Extract stem cell suspension with stem cell cardiac muscle stereo planting device of the present invention, along direction perpendicular to the ischemic myocardium outer surface, the suspension that will contain stem cell evenly slowly injects in the ischemic myocardium, smears the injection site with biological fibrin glue then, prevents that cell suspension from overflowing.Can make like this in the internal layer, middle level, outer cardiac muscle of ischemic myocardium all has stem cell, reaches even plantation stem cell, improves the purpose of cardiac function to greatest extent.
Advantage of the present invention is: the stem cell plantation that is exclusively used in ischemic myocardium.The skin, middle level of cardiac muscle, and internal layer can carry out stereo planting to stem cell, avoid in the conventional dry cell seeding process improving cardiac function better because the cell distribution inequality causes stem cell dysplasia in cardiac muscle.
Description of drawings
Fig. 1 is the structural representation of the embodiment of the invention 1
Fig. 2 is the schematic top plan view of the embodiment of the invention 1 arrangement of combination syringe needle in the suspension groove.Represent syringe needle with stain, numeral is stretched out the length of needle stand lower surface for this syringe needle.
Fig. 3 is the schematic top plan view of the embodiment of the invention 2 arrangements of combination syringe needle in the suspension groove.Represent syringe needle with stain, numeral is stretched out the length of needle stand lower surface for this syringe needle.
The specific embodiment
As shown in Figure 1, the present invention is made of combination syringe needle 1, needle stand 2, flexible pipe 3 and syringe 4; One suspension groove 9 is arranged, in the combination syringe needle 1 bottom-up insertion suspension groove 9 in needle stand 2; The joint 5 of flexible pipe 3 joins with the port of export of syringe 4, and the other end of flexible pipe 3 and needle stand 2 join, and are inserted in the suspension groove 9.The syringe end of syringe 4 has a baffle plate 8, and 3 equidistant bayonet sockets 7 are arranged on the piston 6 of syringe 4.
In order to express the structure of present embodiment better in Fig. 1, the joint 5 of flexible pipe 3 and the port of export of syringe 4 are off working state separately.
As shown in Figure 2, the figure shows the arrangement of combination syringe needle in the suspension groove of embodiment 1.This combination syringe needle is made of 2 3.5mm syringe needles, 3 4.5mm syringe needles and 4 6.5mm syringe needles.The head end of each syringe needle becomes blunt round, and the length on syringe needle slope is less than 1mm.
Except that the combination arrangement of syringe needle in the suspension groove and embodiment 1 were different, other was identical with embodiment 1.As shown in Figure 3, the figure shows the arrangement of combination syringe needle in the suspension groove of embodiment 2.This combination syringe needle is made of 2 3.0mm syringe needles, 3 2.0mm syringe needles and 4 5.0mm syringe needles.
Claims (10)
1. a stem cell cardiac muscle stereo planting device is characterized in that: be made of combination syringe needle, needle stand, flexible pipe and syringe; One suspension groove is arranged, in the bottom-up insertion suspension of the combination syringe needle groove in needle stand; The joint of flexible pipe and the port of export of syringe join, and the other end and the needle stand of flexible pipe join, and are inserted in the suspension groove.
2. stem cell cardiac muscle stereo planting device according to claim 1, it is characterized in that: described syringe, its syringe end has a baffle plate, and 2～10 equidistant bayonet sockets are arranged on its piston.
3. stem cell cardiac muscle stereo planting device according to claim 1, it is characterized in that: described needle stand is made by medical plastic.
4. stem cell cardiac muscle stereo planting device according to claim 1, it is characterized in that: the head end of described combination syringe needle becomes blunt round.
5. stem cell cardiac muscle stereo planting device according to claim 1, it is characterized in that: described combination syringe needle is made of 9 pins.
6. stem cell cardiac muscle stereo planting device according to claim 1, it is characterized in that: described combination syringe needle is made of 16 pins.
7. stem cell cardiac muscle stereo planting device according to claim 5, it is characterized in that: described combination syringe needle is that 3.5mm syringe needle, 3 length are that 4.5mm syringe needle and 4 length constitute for the 6.5mm syringe needle by 2 length.
8. stem cell cardiac muscle stereo planting device according to claim 5, it is characterized in that: described combination syringe needle is that 3.0mm syringe needle, 3 length are that 2.0mm syringe needle and 4 length constitute for the 5.0mm syringe needle by 2 length.
9. the preparation method of the described stem cell cardiac muscle of a claim 1 stereo planting device, it is characterized in that described needle stand is made by following method: choosing length is 1 * 1 * 0.8cm
3Medical grade plastic materials, make a call to 9 or 16 pin holes with laser along the vertical planar direction of needle stand, the syringe needle 9 that grinds or 16 are installed promptly.
10. the using method of the described stem cell cardiac muscle of a claim 1 stereo planting device, it is characterized in that: extract stem cell suspension with stem cell cardiac muscle stereo planting device earlier, along direction perpendicular to the ischemic myocardium outer surface, the suspension that will contain stem cell evenly slowly injects in the ischemic myocardium, smear the injection site with biological fibrin glue then, prevent that cell suspension from overflowing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310121735 CN1255191C (en) | 2003-12-22 | 2003-12-22 | Stem cell cardiac muscle steoro transplanter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310121735 CN1255191C (en) | 2003-12-22 | 2003-12-22 | Stem cell cardiac muscle steoro transplanter |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1554452A CN1554452A (en) | 2004-12-15 |
| CN1255191C true CN1255191C (en) | 2006-05-10 |
Family
ID=34338530
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200310121735 Expired - Fee Related CN1255191C (en) | 2003-12-22 | 2003-12-22 | Stem cell cardiac muscle steoro transplanter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1255191C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101342085B (en) * | 2007-07-09 | 2012-10-10 | 王迎峰 | Apparatus for implantation of tissue sticking patch |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112015014386A2 (en) * | 2012-12-17 | 2017-09-26 | Cormatrix Cardiovascular Inc | intramyocardial agent delivery device and related methods |
| CN112300933B (en) * | 2020-10-30 | 2023-10-03 | 广州迈普再生医学科技股份有限公司 | Organoid molding device and method |
| CN112391273A (en) * | 2020-11-20 | 2021-02-23 | 天康生物股份有限公司 | Single cell separator, application of single cell separator in single cell separation process and preparation method of monoclonal cells |
-
2003
- 2003-12-22 CN CN 200310121735 patent/CN1255191C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101342085B (en) * | 2007-07-09 | 2012-10-10 | 王迎峰 | Apparatus for implantation of tissue sticking patch |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1554452A (en) | 2004-12-15 |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |