CN1367840A - 突变型Tn5转座酶及其使用方法 - Google Patents
突变型Tn5转座酶及其使用方法 Download PDFInfo
- Publication number
- CN1367840A CN1367840A CN00811220.7A CN00811220A CN1367840A CN 1367840 A CN1367840 A CN 1367840A CN 00811220 A CN00811220 A CN 00811220A CN 1367840 A CN1367840 A CN 1367840A
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- transposase
- mutant
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14668699P | 1999-08-02 | 1999-08-02 | |
| US60/146,686 | 1999-08-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1367840A true CN1367840A (zh) | 2002-09-04 |
Family
ID=22518527
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN00811220.7A Pending CN1367840A (zh) | 1999-08-02 | 2000-08-02 | 突变型Tn5转座酶及其使用方法 |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP1198583A1 (pl) |
| JP (1) | JP2003505104A (pl) |
| CN (1) | CN1367840A (pl) |
| AU (1) | AU775043B2 (pl) |
| CA (1) | CA2380850A1 (pl) |
| PL (1) | PL353241A1 (pl) |
| RU (1) | RU2002105508A (pl) |
| WO (1) | WO2001009363A1 (pl) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101698848B (zh) * | 2009-08-26 | 2012-10-03 | 上海海洋大学 | 金鱼自主转座子基因gfTol2、该基因编码的转座酶及该基因的用途 |
| CN103602654A (zh) * | 2013-11-20 | 2014-02-26 | 浙江农林大学 | 人工改造的高活性Mariner-Like转座酶 |
| CN106507677A (zh) * | 2014-04-15 | 2017-03-15 | 伊鲁米那股份有限公司 | 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 |
| CN106754811A (zh) * | 2016-12-21 | 2017-05-31 | 南京诺唯赞生物科技有限公司 | 一种突变型Tn5转座酶及其制备方法和应用 |
| CN108265040A (zh) * | 2017-09-24 | 2018-07-10 | 天津强微特生物科技有限公司 | 一种嗜盐古细菌中的转座酶高活性突变体 |
Families Citing this family (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6159736A (en) * | 1998-09-23 | 2000-12-12 | Wisconsin Alumni Research Foundation | Method for making insertional mutations using a Tn5 synaptic complex |
| US20020072097A1 (en) * | 2000-07-07 | 2002-06-13 | Delcardayre Stephen | Molecular breeding of transposable elements |
| EP1339863A2 (en) | 2000-12-05 | 2003-09-03 | Wisconsin Alumni Research Foundation | Double transposition methods for manipulating nucleic acids |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| HRP20151294T1 (hr) | 2010-04-05 | 2016-03-11 | Prognosys Biosciences, Inc. | Biološka testiranja sa prostornim kodiranjem |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| WO2014060483A1 (en) | 2012-10-17 | 2014-04-24 | Spatial Transcriptomics Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| EP3013983B1 (en) | 2013-06-25 | 2023-02-15 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| US10774374B2 (en) | 2015-04-10 | 2020-09-15 | Spatial Transcriptomics AB and Illumina, Inc. | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| KR20250143875A (ko) | 2017-02-21 | 2025-10-02 | 일루미나, 인코포레이티드 | 링커를 갖는 고정된 트랜스포좀을 사용한 태그먼트화 |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| EP3844304B1 (en) | 2018-08-28 | 2024-10-02 | 10X Genomics, Inc. | Methods for generating spatially barcoded arrays |
| EP4477758B1 (en) | 2018-11-30 | 2026-04-22 | Illumina, Inc. | Analysis of multiple analytes using a single assay |
| EP3894590B1 (en) | 2018-12-10 | 2025-10-22 | 10X Genomics, Inc. | Methods of using master / copy arrays for spatial detection |
| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| JP7571012B2 (ja) | 2018-12-17 | 2024-10-22 | イルミナ インコーポレイテッド | 配列決定用ライブラリーを調製するための方法および手段 |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| MX2021007774A (es) | 2019-07-12 | 2021-08-05 | Illumina Cambridge Ltd | Preparacion de genoteca de acido nucleico usando electroforesis. |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| WO2021133849A1 (en) | 2019-12-23 | 2021-07-01 | 10X Genomics, Inc. | Methods for spatial analysis using rna-templated ligation |
| CN115135984B (zh) | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| ES2965354T3 (es) | 2020-04-22 | 2024-04-12 | 10X Genomics Inc | Métodos para análisis espacial que usan eliminación de ARN elegido como diana |
| EP4153775B1 (en) | 2020-05-22 | 2024-07-24 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| EP4553168A3 (en) | 2020-05-22 | 2025-08-27 | 10x Genomics, Inc. | Spatial analysis to detect sequence variants |
| WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| EP4165207B1 (en) | 2020-06-10 | 2024-09-25 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
| EP4172362B1 (en) | 2020-06-25 | 2024-09-18 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| EP4729631A2 (en) | 2020-12-21 | 2026-04-22 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| US20240093290A1 (en) | 2021-01-29 | 2024-03-21 | 10X Genomics, Inc. | Method for transposase mediated spatial tagging and analyzing genomic dna in a biological sample |
| EP4428246B1 (en) | 2021-04-14 | 2025-12-24 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| EP4582555A3 (en) | 2021-06-03 | 2025-10-22 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| ES3011462T3 (en) | 2021-09-01 | 2025-04-07 | 10X Genomics Inc | Methods for blocking a capture probe on a spatial array |
| WO2023086880A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| EP4305195A2 (en) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| WO2023225519A1 (en) | 2022-05-17 | 2023-11-23 | 10X Genomics, Inc. | Modified transposons, compositions and uses thereof |
| WO2024102809A1 (en) | 2022-11-09 | 2024-05-16 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of multiple analytes in a biological sample |
-
2000
- 2000-08-02 CN CN00811220.7A patent/CN1367840A/zh active Pending
- 2000-08-02 PL PL00353241A patent/PL353241A1/pl not_active Application Discontinuation
- 2000-08-02 AU AU63967/00A patent/AU775043B2/en not_active Ceased
- 2000-08-02 WO PCT/US2000/021052 patent/WO2001009363A1/en not_active Ceased
- 2000-08-02 EP EP00950940A patent/EP1198583A1/en not_active Withdrawn
- 2000-08-02 JP JP2001513619A patent/JP2003505104A/ja active Pending
- 2000-08-02 RU RU2002105508/13A patent/RU2002105508A/ru not_active Application Discontinuation
- 2000-08-02 CA CA002380850A patent/CA2380850A1/en not_active Abandoned
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101698848B (zh) * | 2009-08-26 | 2012-10-03 | 上海海洋大学 | 金鱼自主转座子基因gfTol2、该基因编码的转座酶及该基因的用途 |
| CN103602654A (zh) * | 2013-11-20 | 2014-02-26 | 浙江农林大学 | 人工改造的高活性Mariner-Like转座酶 |
| CN103602654B (zh) * | 2013-11-20 | 2016-02-24 | 浙江农林大学 | 人工改造的高活性Mariner-Like转座酶 |
| CN106507677A (zh) * | 2014-04-15 | 2017-03-15 | 伊鲁米那股份有限公司 | 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 |
| CN106507677B (zh) * | 2014-04-15 | 2021-03-12 | 伊鲁米那股份有限公司 | 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 |
| CN113005108A (zh) * | 2014-04-15 | 2021-06-22 | 伊鲁米那股份有限公司 | 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 |
| CN113005108B (zh) * | 2014-04-15 | 2025-02-11 | 伊鲁米那股份有限公司 | 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 |
| CN106754811A (zh) * | 2016-12-21 | 2017-05-31 | 南京诺唯赞生物科技有限公司 | 一种突变型Tn5转座酶及其制备方法和应用 |
| CN106754811B (zh) * | 2016-12-21 | 2019-05-14 | 南京诺唯赞生物科技有限公司 | 一种突变型Tn5转座酶及其制备方法和应用 |
| CN108265040A (zh) * | 2017-09-24 | 2018-07-10 | 天津强微特生物科技有限公司 | 一种嗜盐古细菌中的转座酶高活性突变体 |
| CN108265040B (zh) * | 2017-09-24 | 2021-02-09 | 天津强微特生物科技有限公司 | 一种嗜盐古细菌中的转座酶高活性突变体 |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2002105508A (ru) | 2004-01-27 |
| EP1198583A1 (en) | 2002-04-24 |
| JP2003505104A (ja) | 2003-02-12 |
| WO2001009363A1 (en) | 2001-02-08 |
| AU6396700A (en) | 2001-02-19 |
| AU775043B2 (en) | 2004-07-15 |
| PL353241A1 (pl) | 2003-11-03 |
| CA2380850A1 (en) | 2001-02-08 |
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