CN1775302A - Preparation method of chitosan-gelatin sponge wound dressing - Google Patents
Preparation method of chitosan-gelatin sponge wound dressing Download PDFInfo
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- CN1775302A CN1775302A CN 200510101409 CN200510101409A CN1775302A CN 1775302 A CN1775302 A CN 1775302A CN 200510101409 CN200510101409 CN 200510101409 CN 200510101409 A CN200510101409 A CN 200510101409A CN 1775302 A CN1775302 A CN 1775302A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 229920001661 Chitosan Polymers 0.000 claims abstract description 18
- 108010010803 Gelatin Proteins 0.000 claims abstract description 13
- 235000019322 gelatine Nutrition 0.000 claims abstract description 13
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 8
- 239000003431 cross linking reagent Substances 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 18
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical group O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 6
- 239000004014 plasticizer Substances 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N glycerol group Chemical group OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 4
- LEQAOMBKQFMDFZ-UHFFFAOYSA-N glyoxal Chemical compound O=CC=O LEQAOMBKQFMDFZ-UHFFFAOYSA-N 0.000 claims description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 4
- 239000013078 crystal Substances 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
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- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 2
- 206010061218 Inflammation Diseases 0.000 claims description 2
- 241000475481 Nebula Species 0.000 claims description 2
- 239000002202 Polyethylene glycol Substances 0.000 claims description 2
- 230000000740 bleeding effect Effects 0.000 claims description 2
- 230000004438 eyesight Effects 0.000 claims description 2
- 235000019253 formic acid Nutrition 0.000 claims description 2
- 235000011187 glycerol Nutrition 0.000 claims description 2
- 229940015043 glyoxal Drugs 0.000 claims description 2
- 230000004054 inflammatory process Effects 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 238000000465 moulding Methods 0.000 claims description 2
- 229920001223 polyethylene glycol Polymers 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
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- 238000005469 granulation Methods 0.000 claims 1
- 230000003179 granulation Effects 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract 2
- 238000001816 cooling Methods 0.000 abstract 1
- 229920002101 Chitin Polymers 0.000 description 4
- 229960000583 acetic acid Drugs 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 239000012567 medical material Substances 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
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- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
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- 230000004069 differentiation Effects 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 210000001126 granulation tissue Anatomy 0.000 description 1
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- 230000035876 healing Effects 0.000 description 1
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Abstract
Description
技术领域 本发明是属于医用材料制备技术领域的一种生物医用敷料的制备方法。Technical Field The present invention is a preparation method of a biomedical dressing, which belongs to the technical field of medical material preparation.
技术背景 甲壳素又名甲壳质、几丁质，其大量存在于虾、蟹等海洋节肢动物的甲壳中，也存在于低等动物菌类、昆虫、藻类的细胞膜和高等植物的细胞壁中，分布极其广泛，壳聚糖是甲壳素经乙酰化反应后得到的可溶性物质，由于其具有良好的生物相容性、生物可降解性，和良好的成膜性、抗凝血性、促进伤口愈合和防腐抗菌等功能，作为医用材料备受关注；明胶是动物胶原经温和断裂后的产物，主要成份是胶原、少量的蛋白多糖及某些有机成份。胶原能参与细胞的迁移、分化和增殖，使骨、腱、软骨和皮肤具有一定的机械强度。因其弱的抗原性和良好的生物相容性，在烧伤、创伤、眼角膜疾病、美容、矫形、硬组织修复、创面止血等医药卫生领域用途广泛。Technical background Chitin, also known as chitin and chitin, exists in large quantities in the shells of marine arthropods such as shrimps and crabs, and also exists in the cell membranes of fungi, insects, algae and cell walls of higher plants. Extremely wide, chitosan is a soluble substance obtained after acetylation of chitin, due to its good biocompatibility, biodegradability, and good film-forming, anticoagulant, wound healing and antiseptic properties Antibacterial and other functions, as a medical material has attracted much attention; gelatin is the product of animal collagen after mild fracture, the main components are collagen, a small amount of proteoglycan and some organic components. Collagen can participate in the migration, differentiation and proliferation of cells, so that bone, tendon, cartilage and skin have certain mechanical strength. Because of its weak antigenicity and good biocompatibility, it is widely used in medical and health fields such as burns, trauma, corneal diseases, cosmetology, orthopedics, hard tissue repair, and wound hemostasis.
目前技术中，有文献报道了一种将壳聚糖溶液冷冻制备高孔隙率膜的方法。也有一些壳聚糖-明胶止血海绵、膜或敷料的研制方法。本研究使用壳聚糖、明胶二者复合，利用各自优点，并且在其中加入具有清热解毒、杀菌消炎、止血生肌、去翳明目等功效的珍珠粉。既可以增加药效，又可以中和醋酸的酸性，还可以使水的晶片变得细小和均匀，从而形成具有独特膜孔结构的海绵状材料。In the current technology, there is a document reporting a method for preparing a high-porosity membrane by freezing a chitosan solution. There are also some chitosan-gelatin hemostatic sponges, membranes or dressings developed. In this study, chitosan and gelatin were used to compound each other, taking advantage of their respective advantages, and adding pearl powder which has the functions of clearing away heat and detoxifying, sterilizing and reducing inflammation, stopping bleeding and promoting muscle growth, removing nebula and improving eyesight. It can not only increase the efficacy of medicine, but also neutralize the acidity of acetic acid, and also make the crystals of water smaller and uniform, thus forming a sponge-like material with a unique membrane pore structure.
发明内容 本发明的技术方案如下：本发明是属于医用材料制备技术领域的一种生物医用敷料的制备方法，其特征在于该方法包括如下步骤：SUMMARY OF THE INVENTION The technical solution of the present invention is as follows: the present invention is a preparation method of a biomedical dressing belonging to the technical field of medical material preparation, which is characterized in that the method comprises the following steps:
(1)壳聚糖、明胶溶液的配成质量分数为2％～3％溶液，其中壳聚糖溶液以弱酸、明胶以水为溶剂；(1) chitosan and gelatin solution are made into a mass fraction of 2% to 3% solution, wherein chitosan solution is solvent with weak acid and gelatin with water;
(2)将壳聚糖溶液分别与明胶溶液以壳聚糖不同比例：壳聚糖/明胶＝4∶1～1∶4(质量比)混合，静置脱泡；(2) Chitosan solution is mixed with gelatin solution in different ratios of chitosan: chitosan/gelatin=4: 1～1: 4 (mass ratio), and stand for defoaming;
(3)分别移取一定量不同比例的混合液，加入少量细珍珠粉，配入少量交联剂和增塑剂，放入成型模具中负压脱泡，-40℃低温下骤冷、固化，取出用蒸馏水冲洗至pH值≈7，用滤纸吸干水分，风干至湿度≈15％，经消毒后备用。(3) Pipette a certain amount of mixed solution in different proportions, add a small amount of fine pearl powder, add a small amount of crosslinking agent and plasticizer, put it into a molding mold for negative pressure defoaming, and quench and solidify at a low temperature of -40°C , take it out and rinse it with distilled water until the pH value is ≈7, blot the water with filter paper, air-dry to the humidity of ≈15%, and disinfect it for later use.
本发明配制壳聚糖所使用的弱酸采用稀盐酸、醋酸、甲酸中的任一种；所用交联剂为甲醛或乙二醛，增塑剂为甘油或乙二醇或聚乙二醇。The weak acid used for preparing chitosan in the present invention adopts any one of dilute hydrochloric acid, acetic acid and formic acid; the crosslinking agent used is formaldehyde or glyoxal, and the plasticizer is glycerin or ethylene glycol or polyethylene glycol.
本发明的技术特征还在于：加入具有特别功效的珍珠粉。既可以增加药效，又可以中和醋酸的酸性，还可以使水的晶片变得细小和均匀，从而形成具有独特膜孔结构的海绵状材料。The technical feature of the present invention also lies in: adding pearl powder with special effects. It can not only increase the efficacy of medicine, but also neutralize the acidity of acetic acid, and also make the crystals of water smaller and uniform, thus forming a sponge-like material with a unique membrane pore structure.
本发明与现有技术相比，具有以下优点：本发明工艺简单，成本低，所制备敷料具有质地柔软、吸水性、抗菌性、促进皮肤愈合、生物相容性和生物可降解性等特性，易于换洗且可防粘连，不易形成疤痕，抗菌消炎且能加快肉芽组织生长，对伤口愈合有很好的治疗效果。由于其具有携带方便，容易清除等特点，所以不仅适用于中小型医院，也适用于部队、农村或者山区救治创烧伤等病人。因此，本发明具有重要的实用价值和经济效益。Compared with the prior art, the present invention has the following advantages: the process of the present invention is simple, the cost is low, and the prepared dressing has the characteristics of soft texture, water absorption, antibacterial property, promotion of skin healing, biocompatibility and biodegradability, etc. It is easy to change and wash and can prevent adhesion, not easy to form scars, antibacterial and anti-inflammatory, and can accelerate the growth of granulation tissue, and has a good therapeutic effect on wound healing. Because it is easy to carry and easy to remove, it is not only suitable for small and medium-sized hospitals, but also suitable for treating patients with wounds and burns in the army, rural areas or mountainous areas. Therefore, the present invention has important practical value and economic benefit.
具体实施方式：本发明实施例：称量10克壳聚糖，溶解于适量1～3％(质量分数)冰乙酸水溶液中配制成质量分数为2～3％的溶液，室温下搅拌至完全溶解，静置过夜。称量10克明胶于适量蒸馏水中浸泡，水浴加热溶解，制得质量分数为2～3％溶液，搅拌后离心，去除沉淀。将壳聚糖溶液分别与明胶溶液以壳聚糖质量分数为20～80％比例混合，加入少量细珍珠粉，配入少量交联剂(重量比为0.1～0.5％)和增塑剂(重量比为0.1～0.5％)，放入成型模具中负压脱泡，-40℃低温下骤冷、固化，取出用蒸馏水冲洗至pH值≈7，用滤纸吸干水分，风干至湿度≈15％，经消毒后备用。The specific embodiment: embodiment of the present invention: weigh 10 grams of chitosan, be dissolved in an appropriate amount of 1 to 3% (mass fraction) glacial acetic acid aqueous solution and be mixed with a mass fraction of 2 to 3% solution, stir at room temperature until fully dissolved , let stand overnight. Weigh 10 grams of gelatin, soak it in an appropriate amount of distilled water, heat and dissolve in a water bath to obtain a solution with a mass fraction of 2-3%, centrifuge after stirring, and remove the precipitate. The chitosan solution is mixed with the gelatin solution so that the mass fraction of chitosan is 20-80%, a small amount of fine pearl powder is added, and a small amount of cross-linking agent (0.1-0.5% by weight) and plasticizer (weight ratio) are added. ratio of 0.1 to 0.5%), put it into the forming mold for negative pressure defoaming, quench and solidify at a low temperature of -40°C, take it out and rinse it with distilled water until the pH value is ≈7, absorb the water with filter paper, and air dry to a humidity of ≈15% , ready for use after disinfection.
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| CN 200510101409 CN1775302A (en) | 2005-11-23 | 2005-11-23 | Preparation method of chitosan-gelatin sponge wound dressing |
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| CN 200510101409 CN1775302A (en) | 2005-11-23 | 2005-11-23 | Preparation method of chitosan-gelatin sponge wound dressing |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101879324A (en) * | 2010-07-02 | 2010-11-10 | 西南大学 | Preparation method of chitosan composite medical dressing |
| CN101955670A (en) * | 2010-09-13 | 2011-01-26 | 福州大学 | Gelatin-chitosan composite food packaging film and preparation method thereof |
| CN102389584A (en) * | 2011-11-08 | 2012-03-28 | 武汉纺织大学 | Konjac sponge medical dressing and preparation method thereof |
| CN103656730A (en) * | 2013-12-10 | 2014-03-26 | 徐花 | Medical chitosan dressing |
| CN105400214A (en) * | 2015-11-30 | 2016-03-16 | 北京化工大学 | Method for preparing hemostatic cotton through hyaluronic acid crosslinked gelatin |
| CN105536029A (en) * | 2015-12-25 | 2016-05-04 | 蓝广芊 | Preparation method for chitosan porous hemostatic sponge |
| CN107970486A (en) * | 2017-12-12 | 2018-05-01 | 常州凯恒纺织品有限公司 | A kind of preparation method of chitosan-based compound hemostatic film |
| CN109124877A (en) * | 2018-09-07 | 2019-01-04 | 南京斯瑞奇医疗用品有限公司 | A kind of tearable self-adhesive elastic bandage in four sides |
| CN115887732A (en) * | 2022-12-23 | 2023-04-04 | 稳得希林(沈阳)生物科技有限公司 | Medical gelatin dressing and preparation method thereof |
-
2005
- 2005-11-23 CN CN 200510101409 patent/CN1775302A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101879324A (en) * | 2010-07-02 | 2010-11-10 | 西南大学 | Preparation method of chitosan composite medical dressing |
| CN101955670A (en) * | 2010-09-13 | 2011-01-26 | 福州大学 | Gelatin-chitosan composite food packaging film and preparation method thereof |
| CN102389584A (en) * | 2011-11-08 | 2012-03-28 | 武汉纺织大学 | Konjac sponge medical dressing and preparation method thereof |
| CN102389584B (en) * | 2011-11-08 | 2014-04-16 | 武汉纺织大学 | Preparation method of konjac sponge medical dressing |
| CN103656730A (en) * | 2013-12-10 | 2014-03-26 | 徐花 | Medical chitosan dressing |
| CN105400214A (en) * | 2015-11-30 | 2016-03-16 | 北京化工大学 | Method for preparing hemostatic cotton through hyaluronic acid crosslinked gelatin |
| CN105536029A (en) * | 2015-12-25 | 2016-05-04 | 蓝广芊 | Preparation method for chitosan porous hemostatic sponge |
| CN105536029B (en) * | 2015-12-25 | 2018-07-13 | 蓝广芊 | A kind of preparation method of chitosan multi-porous styptic sponge |
| CN107970486A (en) * | 2017-12-12 | 2018-05-01 | 常州凯恒纺织品有限公司 | A kind of preparation method of chitosan-based compound hemostatic film |
| CN109124877A (en) * | 2018-09-07 | 2019-01-04 | 南京斯瑞奇医疗用品有限公司 | A kind of tearable self-adhesive elastic bandage in four sides |
| CN115887732A (en) * | 2022-12-23 | 2023-04-04 | 稳得希林(沈阳)生物科技有限公司 | Medical gelatin dressing and preparation method thereof |
| CN115887732B (en) * | 2022-12-23 | 2024-03-22 | 稳得希林(沈阳)生物科技有限公司 | Medical gelatin dressing and preparation method thereof |
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