CN203451653U - Interlayer type electrostatic spinning nozzle - Google Patents

Abstract

The utility model discloses a sandwich type electrostatic spinning nozzle. The sandwich type electrostatic spinning nozzle includes at least one single nozzle. Tube. The utility model has the advantages of simple structure and convenient operation, and can prepare regenerated silk fibroin nanofiber yarn with the property of being insoluble in water.

Description

Sandwich Electrospinning Nozzle

technical field

The utility model relates to the field of electrostatic spinning, in particular to a sandwich type electrostatic spinning nozzle.

Background technique

Silk fibroin, as a natural polymer protein, is rich in sources, has good biocompatibility and degradability, is non-toxic, non-irritating, and has low inflammatory response. It has long been used as surgical sutures. Electrospinning technology is currently one of the most effective and fast ways to prepare nanofibers. It has developed rapidly because of its advantages of simple device, convenient operation and low cost. Since electrospinning can directly and effectively prepare continuous micro-nano fibers similar to the structure of natural extracellular matrix, the construction of silk fibroin nano-biomedical materials and tissue engineering scaffolds by electrospinning technology has become a new research field of silk fibroin and electrospinning research. hot spot.

However, silk protein materials prepared from regenerated silk protein solutions are easily soluble in water, so physical or chemical methods need to be used to make them insoluble through conformational transformation. Such as heat or humidity treatment, organic solvent treatment or stretching of the sample. For example, if the electrospun silk fibroin/polyoxyethylene nascent nanofiber mat is placed at a humidity of 95% and a temperature of 25% for 72 hours, the molecular conformation is transformed into a stable β-fold structure; the concentrated silk fibroin aqueous solution is After electrospinning, the as-spun silk was treated with 90/10 methanol. As a result, the silk fibroin changed from random coil to β-sheet structure; the silkworm/tussah silkworm nanofibers obtained by electrospinning were treated with 75% ethanol and used as a scaffold The material is subjected to cell culture; the obtained silk fibroin nanofiber mat is immersed in 90% ethanol and stretched under tension at the same time, and a silk material with better mechanical properties and water stability is also obtained.

Although post-treatment of electrospun fibroin nanofibers can induce their transformation into hydrophobic materials, there are disadvantages such as complicated preparation process, cumbersome operation and long post-treatment time, and electrospun fibroin nanofibers are mostly single fibers arranged in disorder , rather than fiber aggregates or fiber yarns with a certain strength and orientation.

Contents of the invention

The purpose of the utility model is to provide a sandwich type electrostatic spinning nozzle with simple structure and convenient operation.

In order to achieve the above object, the technical solution adopted by the utility model is: a sandwich type electrospinning nozzle, including at least one single nozzle, and each single nozzle includes a hollow nozzle, and the nozzle is concentrically nested There are at least two hollow inner nozzles, and the nozzles have the same cross-sectional shape as the inner nozzles.

In the above technical solution, the shape of the spray chamber and the inner spray chamber can be a cylinder, a cone, a frustum of a cone, a cuboid, an irregular cuboid with a small top and a large bottom, or a large bottom and a small top.

In a preferred technical solution, the outlet end of the nozzle is provided with a nozzle, and the outlet end of the inner nozzle is provided with an inner nozzle.

In a further technical solution, the nozzle diameter is 0.01cm-1cm, and the preferred nozzle diameter is generally 0.01cm-0.1cm in actual use.

In a preferred technical solution, the length of the nozzle is 1cm-50cm.

In a preferred technical solution, there are 1-50 single spray heads.

The method of using the sandwich type electrospinning nozzle is to inject coagulation liquid into the nozzle, inject regenerated silk fibroin spinning liquid and coagulation liquid into each inner nozzle, and pressurize to make the coagulation liquid and regenerated silk fibroin spinning liquid The coagulation liquid will insoluble treat the regenerated silk fibroin spinning liquid, and will be stretched and thinned under the action of electrostatic to form insoluble regenerated silk fibroin nanofiber yarn; the coagulation liquid will not completely volatilize , has been accompanied by the spinning process, and will restrict the separation of the split nanofibers until they are completely volatilized, thereby forming regenerated silk nanofiber yarns. By adjusting the positions of the inner nozzles, the contact time between the coagulation liquid and the regenerated silk fibroin spinning liquid can be changed, thereby increasing the insoluble treatment effect; by changing the type and concentration of the coagulation liquid, regenerated silk fibroin with different shapes and diameters can be produced nanofiber yarn.

The mass concentration of the regenerated silk fibroin spinning solution is 1% to 40%. The regenerated silk fibroin spinning solution can be regenerated silk fibroin proteins such as silkworm silk, tussah silk, celestial silk, spider silk, etc.; the coagulation solution can be methanol, ethanol and other organic Alcohols, glutaraldehyde, genipin, and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) crosslinkers.

Due to the application of the above-mentioned technical solutions, the utility model has the following advantages compared with the prior art:

1. The utility model has the advantages of simple structure, convenient operation, short technological process and no post-processing;

2. The utility model can directly obtain nanofiber yarn composed of regenerated silk fibroin nanofibers in one step, without adding other aggregation methods, and performing processes such as fiber merging, collection, and drafting;

3. The utility model can change the number of nozzles according to the needs, thereby increasing the output and ensuring the treatment effect of each nozzle.

Description of drawings

Fig. 1 is a schematic diagram of a single nozzle structure;

Fig. 2 is a longitudinal sectional view of a single nozzle;

Figure 3 is a schematic diagram of electrospinning multi-nozzles.

Among them: 1. Single nozzle; 2. Nozzle; 3. Inner nozzle; 4. Nozzle; 5. Inner nozzle; 6. Outer inner nozzle; 7. Inner inner nozzle.

Detailed ways

Below in conjunction with accompanying drawing and embodiment the utility model is further described:

Embodiment one:

As shown in Figures 1-3, a sandwich type electrospinning nozzle includes five single nozzles 1, and the single nozzle 1 also includes a hollow nozzle 2, and two hollow inner nozzles are nested concentrically in the nozzle 2. The pipe 3, the nozzle pipe 2 and the inner nozzle pipe 3 have the same cross-sectional shape.

The outlet end of the nozzle pipe 2 is provided with a nozzle 4 , and the outlet end of the inner nozzle pipe 3 is provided with an inner nozzle 5 .

The nozzle 4 caliber is 0.05cm.

The length of the nozzle 2 is 5 cm.

A method for preparing regenerated silk fibroin nanofiber yarn using a sandwich type electrospinning nozzle:

(1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and the pure mulberry silk fibroin protein fiber was obtained after repeated 3 times. The silk fibroin was dissolved in 9.3 M/L LiBr and stirred in a water bath at a constant temperature of 55±2° C. for 4 hours to obtain a regenerated silk fibroin solution after dissolving. After 3 days of dialysis, dry naturally to make a regenerated silk fibroin film;

(2) Dissolve the regenerated silk fibroin film prepared in step (1) in formic acid to form a silk spinning solution with a concentration of 12%, inject it into the inner nozzle 6 of the outer layer, and use 75% ethanol solution as a coagulation Liquid is injected into the nozzle 2 and the inner nozzle 7 respectively, and the three injection channels are squeezed at the same time, and the injection rate is 0.2ml/h. Under the condition of voltage of 15KV and collection distance of 12cm, the insoluble regenerated silk fibroin nanofiber yarn was directly prepared.

Claims (5)

1. a sandwich-type electrostatic spinning nozzle, it is characterized in that: comprise at least 1 single spraying head, described in each, single spraying head comprises again the jet pipe of hollow, and in described jet pipe, nested, concentric has at least 2 hollow inner nozzles, and described jet pipe is identical with described inner nozzle shape of cross section.

2. a kind of sandwich-type electrostatic spinning nozzle according to claim 1, is characterized in that: described nozzle exit end is provided with spout, and the described inner nozzle port of export is provided with interior spout.

3. a kind of sandwich-type electrostatic spinning nozzle according to claim 2, is characterized in that: described spout bore is 0.01cm-1cm.

4. a kind of sandwich-type electrostatic spinning nozzle according to claim 1, is characterized in that: described jet pipe length is 1cm-50cm.

5. a kind of sandwich-type electrostatic spinning nozzle according to claim 1, is characterized in that: described single spraying head is provided with 1-50.

Images