CN204017444U - A kind of efficient magnetic control disperses solid-phase extraction device - Google Patents

Abstract

A kind of efficient magnetic control disperses solid-phase extraction device, it is characterized in that: the extraction cartridge that can simultaneously carry out batch process sample that comprises several settings parallel with one another, the upper end of extraction cartridge is provided with injection port, lower end is provided with outlet, the in-built micron adsorbent that is magnetic of extraction cartridge, the bottom in extraction cartridge is provided with sieve plate, and sieve plate is provided with ultrasonic, extraction cartridge is around with coil, and the end line of coil and power supply, power switch and slide rheostat are in series.The utility model patent has overcome traditional dispersion solid phase extraction techniques complex operation, poor reproducibility, extraction is slow and extraction efficiency is low shortcoming, the ultrasonic absorption of the utility model collection, purification, wash-out and solid-liquid two-phase magnetic are located away from one, the single or multiple mass simultaneous processing samples of energy, have feature simple to operate, that rate of extraction is fast, efficiency is high.

Description

A high-efficiency magnetron dispersion solid-phase extraction device

technical field

The utility model belongs to the technical field of solid phase extraction, and relates to a sample analysis pretreatment device, in particular to a high-efficiency magnetic control dispersion solid phase extraction device, which is used for uniform dispersion, adsorption and desorption of magnetic adsorption materials during dispersive solid phase extraction. Rapid separation of solid/liquid two phases.

Background technique

Due to the complexity of the analysis sample matrix, the research of new and efficient sample pretreatment technology is particularly important. Dispersive solid phase extraction technology is a kind of enrichment and purification sample pretreatment technology developed rapidly in recent years. limit. The history of magnetic separation can be traced back more than 100 years ago. In 1865, Porter first applied it to large-scale water treatment equipment. Later, it was gradually applied to the fields of microbiology, cell biology, molecular biology and biochemistry, analytical chemistry, biotechnology and environmental protection technology. Based on the difference in the physical adsorption process, magnetic separation is mainly divided into four forms: direct magnetic separation, magnetic separation with flocculant added, separation with magnetic adsorbent (ie magnetic control solid phase extraction), and magnetic separation combined with other processes. . The basic principle of magnetically controlled solid-phase extraction is to use the two-phase distribution of the target substance between the magnetic adsorption material and the solution. When the sample solution is in contact with the magnetic adsorbent, the enrichment of the target analyte can be achieved through selective adsorption. And its separation from the coexisting components, and then the solid/liquid two phases are separated by magnetic control technology. According to the existence form of the stationary phase, the magnetic solid phase extraction mode can be divided into two types: dispersed type and packed column type. Among them, the dispersion method is mostly carried out in a beaker. After ultrasonically dispersing the nano-magnetic particles, the upper solution can be clarified quickly by applying an external magnetic field, and batch operation can be realized. During the extraction process, when the magnetic material is added to the solution containing the target analyte , the target analyte is selectively adsorbed on the magnetic material, and after an external magnetic field is applied, the magnetic material adsorbed by the target analyte is rapidly separated from the original solution. Subsequently, the target analyte is eluted from the nano-magnetic material with a suitable solvent, thereby realizing the purification and enrichment of the target analyte, and finally the eluate is introduced into a subsequent analysis instrument for detection.

After investigation, the existing patent No. CN201220351885.6 is the Chinese patent "Magnetic Control Magnetic Matrix Solid-Phase Extraction Device". The upper end of the column has an upper opening, and the lower end has a funnel-shaped lower opening. and the coil frame, and the terminal wire of the coil is connected in series with a DC power supply, a switch and a sliding rheostat. The magnetic directional movement is generated by an external magnetic field, so that the extraction speed of the magnetic sample is accelerated in the paramagnetic direction, which overcomes the shortcomings of slow and incomplete extraction during the experiment and saves time. However, its magnetic adsorbent is filled in the column as a solid matrix through a sieve plate. During extraction, the magnetic adsorbent is fixed and cannot be fully dispersed in the sample solution. Therefore, there is a defect of low extraction efficiency, which needs further improvement. The magnetic adsorbent can be uniformly dispersed and adsorbed in the sample solution to realize the rapid magnetic separation of solid/liquid two phases and improve the extraction efficiency.

Utility model content

The technical problem to be solved by the utility model is to provide a high-efficiency magnetic control dispersion solid-phase extraction device integrating ultrasonic adsorption, purification, elution and solid/liquid two-phase magnetic separation, which has the characteristics of simple operation and high extraction efficiency.

The technical scheme adopted by the utility model to solve the above technical problems is: a high-efficiency magnetic control dispersion solid-phase extraction device, which is characterized in that it includes several extraction columns arranged in parallel and can process samples in batches at the same time. The upper end of the column body is provided with a sample inlet, and the lower end is provided with a sample outlet. The extraction column body is equipped with a magnetic micron adsorbent, and the bottom of the extraction column body is equipped with a sieve plate. The sieve plate is equipped with an ultrasonic sheet. A coil is wound outside the column, and the end wire of the coil is connected in series with the power supply, the power switch and the sliding rheostat.

As an improvement, the coil is a half-ring coil wound on the coil skeleton outside the extraction column body, the extraction column body is transparent or a longitudinal transparent window is provided on the extraction column body, and There is a scale on the surface or a transparent window that can display the volume of the solution.

As an improvement, the sample outlet at the lower end of the extraction column body is funnel-shaped and provided with a control valve.

As an improvement, there are 1 to 36 extraction columns.

As a further improvement, there are 1, 6 or 12 extraction columns.

Compared with the prior art, the utility model has the advantages that multiple extraction columns are provided, and samples can be processed in batches at the same time, one or more, and the scale on the surface of the extraction column can accurately measure the volume of the sample to be processed. The ultrasonic sheet at the bottom can ensure the uniform dispersion and adsorption of the magnetic adsorbent in the sample solution, and the control valve at the sample outlet can conveniently control the contact time between the magnetic adsorbent and the sample solution; when working, the magnetic field generated by the energized coil makes each The magnetic micro-adsorbent adsorbed with the target analyte in each column exhibits a certain magnetization intensity under the action of the external magnetic field, generating a magnetic field force, thereby realizing the rapid magnetic separation of the solid/liquid two-phase; the number of turns of the half-ring coil can be determined according to the actual situation It needs to be increased or decreased, and the magnitude of the magnetic field force can be realized by adjusting the sliding rheostat to control the magnitude of the current. The utility model overcomes the shortcomings of traditional dispersive solid-phase extraction technology, such as cumbersome operation, poor reproducibility, slow extraction and low extraction efficiency. The utility model integrates ultrasonic adsorption, purification, elution and solid/liquid two-phase magnetic separation, and has the advantages of It has the characteristics of simple operation, fast extraction speed and high efficiency.

Description of drawings

Fig. 1 is a schematic structural view of the device involved in the utility model patent;

Fig. 2 is a schematic structural view of a single extraction column in Fig. 1 .

Detailed ways

Below in conjunction with accompanying drawing embodiment, the utility model patent is described in further detail.

As shown in Figures 1 and 2, the high-efficiency magnetic control dispersive solid-phase extraction device of the present embodiment includes 6 extraction columns 1 that are arranged in parallel and can simultaneously process samples in batches. The upper end of the extraction column 1 is provided with The sample inlet 11 is provided with a sample outlet at the lower end, and a coil bobbin 6 is arranged outside the column body 1 of the extraction column, and a coil 3 is wound on the frame body 6, and the coils 3 outside the column body 1 of each extraction column are connected in series, and the coils 3 are connected in series. The terminal line is connected in series with the power supply 9, the power switch 10 and the sliding rheostat 8 to form a control loop; the extraction column body 1 is equipped with a magnetic micron adsorbent 7, and the bottom of the extraction column body 1 is provided with a sieve plate 5, and on the sieve plate 5 An ultrasonic sheet 4 is provided. The presence of the ultrasonic sheet 4 enables the magnetic micro-adsorbent 7 to achieve ultrasonic-assisted uniform dispersion in the sample solution. The coil 3 is a semi-circular coil, and the extraction column body 1 is transparent. The outer surface of the extraction column is provided with a scale that can display the volume of the solution, so that the volume of the liquid sample can be accurately measured; the sample outlet at the lower end of the extraction column body 1 is funnel-shaped and is provided with a control valve 2, which can facilitate the control of the magnetic adsorbent and the sample. solution contact time.

The working status of the utility model patent will be further described below by way of examples.

Example 1

1. Dispersion and adsorption: The power switch 10 is off, the coil 3 is not powered, the control valve 2 at the sample outlet is closed, and the ultrasonic sheet 4 is operated. At this time, the magnetic adsorbent 7 is fully dispersed in the sample solution and absorbs the Impurities.

2. Magnetic separation: After fully adsorbing, close the power switch 10, at this time the coil 3 starts to energize and generate a magnetic field, open the sample outlet control valve 2, and collect the liquid sample after absorbing impurities. Effective solid/liquid two-phase magnetic separation is realized under the action.

3. Concentration of the target object: collect the solution, blow it with nitrogen and concentrate it, and conduct qualitative and quantitative analysis according to the requirements after constant volume.

Example 2

1. Dispersion and adsorption: The power switch 10 is off, the coil 3 is not energized, the control valve 2 at the sample outlet is closed, and the ultrasonic sheet 4 is operated. At this time, the magnetic adsorbent 7 is fully dispersed in the sample solution and adsorbs the target.

2. Magnetic separation: After fully adsorbed, close the power switch 10, at this time the coil 3 starts to energize and generate a magnetic field, open the sample outlet control valve 2, and discard the adsorbed liquid sample, the magnetic micron adsorbent 7 is under the action of the magnetic field effective solid/liquid two-phase magnetic separation.

3. Rinse: close the sample outlet control valve 2, disconnect the power switch 10, add impurity eluent from the upper end of the extraction column column body 1, and add impurity eluent, and run the ultrasonic film 4 to make the eluent and magnetic micron adsorbent 7 Fully contact, after a period of time, close the power switch 10, the system generates a magnetic field, open the sample outlet control valve 2, and discard the impurity eluent through the sample outlet.

4. The elution and concentration of the target substance: close the sample outlet control valve 2, disconnect the power switch 10, add the target substance eluent from the injection port 11 at the upper end of the extraction column body 1, run the ultrasonic film 4, and make the target substance The eluent is in full contact with the magnetic micron adsorbent 7, and the target analyte is eluted. At this time, the power switch 10 is closed, the system generates a magnetic field, the control valve 2 of the sample outlet is opened, the eluent is collected, concentrated by nitrogen blowing, and after constant volume Qualitative and quantitative analysis as required.

Claims (5)

1. an efficient magnetic control disperses solid-phase extraction device, it is characterized in that: the extraction cartridge that can simultaneously carry out batch process sample that comprises several settings parallel with one another, the upper end of extraction cartridge is provided with injection port, lower end is provided with outlet, the in-built micron adsorbent that is magnetic of extraction cartridge, the bottom in extraction cartridge is provided with sieve plate, and sieve plate is provided with ultrasonic, extraction cartridge is around with coil, and the end line of coil and power supply, power switch and slide rheostat are in series.

2. efficient magnetic control according to claim 1 disperses solid-phase extraction device, it is characterized in that: described coil is doroid, on coil rack outside extraction cartridge, extraction cartridge is transparence or on extraction cartridge, is provided with longitudinal transparency window, on the outer surface of extraction cartridge or transparency window, is provided with the scale that can show liquor capacity.

3. efficient magnetic control according to claim 1 disperses solid-phase extraction device, it is characterized in that: the lower end outlet of described extraction cartridge is funnel-form and is provided with control valve.

4. efficient magnetic control according to claim 1 disperses solid-phase extraction device, it is characterized in that: described extraction cartridge is 1～36.

5. efficient magnetic control according to claim 4 disperses solid-phase extraction device, it is characterized in that: described extraction cartridge is 1,6 or 12.