CR20210231A - Proteínas inhibidoras de insectos novedosa - Google Patents

Proteínas inhibidoras de insectos novedosa

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Publication number
CR20210231A
CR20210231A CR20210231A CR20210231A CR20210231A CR 20210231 A CR20210231 A CR 20210231A CR 20210231 A CR20210231 A CR 20210231A CR 20210231 A CR20210231 A CR 20210231A CR 20210231 A CR20210231 A CR 20210231A
Authority
CR
Costa Rica
Prior art keywords
tic7941pl
disclosed
pesticidal
proteins
nucleic acid
Prior art date
Application number
CR20210231A
Other languages
English (en)
Inventor
David J Bowen
Yuanji Zhang
Todd A Ciche
Arlene R Howe
Danqi Chen
Barbara E Wiggins
Catherine A Chay
Jennifer L Lutke
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of CR20210231A publication Critical patent/CR20210231A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/25Paenibacillus
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A01H1/1245Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
    • A01H1/127Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance for insect resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10Seeds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44Guanidine; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60Isolated nucleic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00Arthropodicides
    • A01P7/04Insecticides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • CCHEMISTRY; METALLURGY
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Immunology (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Insects & Arthropods (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Urology & Nephrology (AREA)
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  • Developmental Biology & Embryology (AREA)
  • General Physics & Mathematics (AREA)
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Abstract

Se revelan proteínas pesticidas que presentan actividad de toxina contra especies de plaga Lepidopteran, e incluyen, aunque sin limitación a, TIC7941, TIC7941PL_1, TIC7941PL_2, y TIC7941PL_3. Se proporcionan constructos de ADN que contienen una secuencia de ácido nucleico recombinante que codifica una o más de las proteínas pesticidas reveladas. Se proporcionan plantas transgénicas, células vegetales, semilla, y partes de plantas resistentes a infestaciones Lepidopteran que contienen secuencias de ácido nucleico recombinantes que codifican las proteínas pesticidas de la presente invención. También se proporcionan métodos para detectar la presencia de las secuencias de ácido nucleico recombinante o las proteínas de la presente invención en una muestra biológica, y métodos para controlar plagas de especie Lepidopteran usando cualquiera de las proteínas pesticidas TIC7941, TIC7941PL_1, TIC7941PL_2, y TIC7941PL_3. Métodos y composición para mejorar la actividad insecticida de una proteína pesticida contra una especie de plaga de insectos también son revelados. También se revela el método y la composición para reducir la expresión de una proteína pesticida en los tejidos reproductivos de una planta transgénica.
CR20210231A 2019-01-22 2020-01-21 Proteínas inhibidoras de insectos novedosa CR20210231A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962795066P 2019-01-22 2019-01-22
PCT/US2020/014437 WO2020154301A1 (en) 2019-01-22 2020-01-21 Novel insect inhibitory proteins

Publications (1)

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CR20210231A true CR20210231A (es) 2021-08-26

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ID=71608677

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CR20210231A CR20210231A (es) 2019-01-22 2020-01-21 Proteínas inhibidoras de insectos novedosa

Country Status (16)

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US (3) US11266151B2 (es)
EP (1) EP3914608A4 (es)
KR (1) KR20210118055A (es)
CN (2) CN112955555B (es)
AR (1) AR117865A1 (es)
AU (1) AU2020212955B2 (es)
BR (1) BR112021006574A2 (es)
CA (2) CA3115551A1 (es)
CL (2) CL2021001055A1 (es)
CO (1) CO2021006033A2 (es)
CR (1) CR20210231A (es)
EA (1) EA202190969A1 (es)
MX (1) MX2021005363A (es)
PY (1) PY2003745A (es)
UY (1) UY38551A (es)
WO (1) WO2020154301A1 (es)

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US11965169B2 (en) * 2020-08-20 2024-04-23 AG Biomolecules LLC Transgenic safflower event stack IND-1ØØØ3-4 x IND-1ØØ15-7 and methods to use it
WO2022140227A1 (en) 2020-12-21 2022-06-30 Monsanto Technolgy Llc Novel insect inhibitory proteins
UY39585A (es) 2020-12-23 2022-07-29 Monsanto Technology Llc Proteínas que exhiben actividad inhibidora de insectos frente a plagas con importancia agrícola de plantas de cultivo y semillas
KR20230127241A (ko) 2020-12-31 2023-08-31 몬산토 테크놀로지 엘엘씨 신규한 곤충 저해 단백질
CA3203559A1 (en) 2021-01-03 2022-07-21 Heba Mohamed Yassen ABDELGAFFAR Novel affinity constructs and methods for their use
US20240360468A1 (en) * 2021-07-27 2024-10-31 BASF Agricultural Solutions Seed US LLC Viral coat delivery of insect resistance genes in plants
CN116064644B (zh) * 2022-10-10 2023-10-10 中国农业科学院生物技术研究所 SbAGO1b蛋白及其编码基因在调控植物抗虫性中的应用
AR131274A1 (es) * 2022-12-07 2025-03-05 Monsanto Technology Llc Nuevas proteínas inhibidoras de insectos
KR102892247B1 (ko) * 2022-12-20 2025-12-01 대한민국(농촌진흥청장) 조명나방 방제 효과를 갖는 dsRNA 및 이의 용도

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CN119040350A (zh) 2024-11-29
AU2020212955A1 (en) 2021-05-06
CN112955555A (zh) 2021-06-11
EP3914608A1 (en) 2021-12-01
US20250261650A1 (en) 2025-08-21
EA202190969A1 (ru) 2021-10-18
CL2021001055A1 (es) 2021-10-01
CA3115551A1 (en) 2020-07-30
US11266151B2 (en) 2022-03-08
BR112021006574A2 (pt) 2021-08-10
CA3250803A1 (en) 2025-02-24
US20220248686A1 (en) 2022-08-11
US20200229445A1 (en) 2020-07-23
CL2022003221A1 (es) 2023-04-21
EP3914608A4 (en) 2023-05-17
KR20210118055A (ko) 2021-09-29
MX2021005363A (es) 2021-06-30
PY2003745A (es) 2021-01-08
AU2020212955B2 (en) 2025-01-02
WO2020154301A1 (en) 2020-07-30
UY38551A (es) 2020-08-31
AR117865A1 (es) 2021-09-01
US12324437B2 (en) 2025-06-10
CN112955555B (zh) 2024-09-03
CO2021006033A2 (es) 2021-05-20

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