CS214369B1 - Method of increasing egg production in the artificial smear of tin (Tinca tinca) - Google Patents

Abstract

Luliberin, a peptide hypothalamic factor of bovine origin, is used primarily to stimulate the release of the corresponding adenohypophyseal hormones. The invention uses this peptide in artificial spawning and increasing their working fertility in tench (Tinca tinca). The method of application is intramuscular in doses of 50-200 /ug/kg live weight of sexually mature eggs of this fish.

Description

The present invention relates to a method for increasing the production of eggs in an artificial outgrowth of tench (Tinca tinca).

So far, hypophysation has been used to increase the efficiency of smelting of tench, i.e. injection of suapense spread dried dried pituitary carp (Kouřil J. and Chábera S., Bulletin VÚRH, Voňňany 4, 7, 1976). The success of artificial swab in pituitary is dependent on the content of gonatropic hormones, whose concentration is almost undetectable in field conditions. Substitution of this natural, variable-active material material with known doses of a defined primary structure substance that would be synthetically accessible would shift the control of tench swab to a more feasible and reproducible level.

Luliberin is a hypothalamic factor of the peptide nature of the primary structure of pyroglutamines 1-histidyl-traptophanyl-geryl-tyroayl-glycyl-leucyl-arginyl-propyl-glyeinamide (Schally AV et al, Science 173, 1036, 1971) and has some regulatory effects on release hypophyseal gonadotropic hormones. The successful application of luliberin to artificial spawning has been described so far only in the herbivorous fish, white carp, black carp, and white carp (Anonyma, Kexuo Tongbao 20, 43, 1975) ·

The production of eggs in tench (Tinca tinca) can be increased by an artificial swab according to the invention, which comprises administering luliberin intramuscularly at a dose of 50 to 200 mg / kg bodyweight of sexually mature tench (Tinca tinca) before the expected swab. .

Example

The compound used, luliberin of the general formula pyroGlu-His-Trp-Ser-Tyr-Gly-Leu-ArgProGlyNHg, synthetically prepared, was dissolved in physiological solution to a concentration of 1 mg / ml, undiluted 10x and stored at 4 ° C until use. The doses of the substance were chosen so that per 1 kg of fish weight there was 1 ml of solution. Fish, tench roe, were 4-5 years old, weighing 300-1000 g, were kept in groups of 10 pieces in laminated vats and inflow of warmed water. Luliberin was administered to the fish at a dose of 100 µg / kg intramuacularly (group A). The control group was tench, which received a suspension of carp-derived pituitary glands at a dose of 5.0 mg / kg of fish weight (group B), and another group of eggs. who received an injection of phyaiological solution (1 ml / kg body weight) - group C. After 15 hours of application, the fish were checked for maturity and readiness for spawning.

Table I shows the results of the injectable administration of luliberin, a pituitary carp suspension and a saline solution. The effect of these applications was monitored by the number of fish spawned, the total weight of eggs, the relative weight and the time needed for spawning.

214 V,

Table X. Effect of luliberin on egg production in tench in artificial smear

Group Number fish Number mopping fish Smear jiker 8 and/ Relative mass Time h AND 10 7 336 5.42 25,55-32,40 (B) 10 8 163 2.74 19,35-25-45 C 10 0 - - -

a / Time of start and end of artificial swab in the group (hours).

The spawned eggs were fertilized by male mice in the usual manner and after peeling incubated in Zugek bottles. Fertilization of eggs of individual groups, incubation and survival of fry to 4 days of age were in normal values. The eggs that only received saline were kept under artificial conditions for a longer period of time and monitored for the next 14 days during which they did not spawn.

Claims (1)

A method of increasing egg production in an artificial swab of tench (Tinea tinca), characterized in that prior to the expected swab ae, administer intramuscularly luliberin at a dose of 50 to 200 µg / 1 kg bodyweight of sexually mature tench (Tinca tinca).