CS232777B1 - Compound feed for livestock, enriched with proteases, especially alkaline, and a process for its production - Google Patents

Abstract

The feed mixture contains enzymes produced by mutants of Bacillus coagulans strains from the Laktoflora collection No. 476 and 477 and Bacillus cereus from the Laktoflora collection No. 478 and 479. The enriched feed mixture significantly increases feed conversion, especially in chickens, laying hens and pigs.

Description

It is an object of the present invention to provide a compound feed for livestock, enriched with proteases, predominantly alkaline, produced by culturing mutants of Bacillus coagulans from Lactoflora Nos. 476 and 477 and Bacillus cereus from Lactoflora Nos. 478 and 479.

It is known to increase feed conversion using pure proteases produced primarily by certain species and strains of fungi.

The application of microbial rennet Mikrozyn, which is obtained by fermentation of sporulating Bacillus sutotilis strains and rennet obtained by production of Bacillus megaterium strains, has also been described. A disadvantage of the first method is the possibility of the formation of some health-defective mycotoxins, especially aflatoxins, and the deficiency of all the existing methods is the production of relatively large amounts of neutral and acidic proteases in addition to alkaline proteases.

These drawbacks are overcome by the animal feed compound and the process according to the invention, which is based on the enrichment of proteases, predominantly alkaline, produced by Bacillus coagulans mutants from Lactoflora Nos. 476 and 477 and Bacillus cereus from Lactoflora Nos. 478 and 479, applied into the compound feed either with the culture liquid containing these enzymes or after separation and concentration by concentration.

The protease enzymes produced by said mutants provide for a deeper cleavage of high molecular weight substances in feed such as proteins in the digestive tract of livestock, especially monogastric, particularly poultry and pigs. This leads to better utilization of nutrients in compound feed. An increase in feed conversion thus results in a reduction in feed consumption per unit of increment and an increase in weight gain per head and day.

Protease enzymes can be added to the feed mixtures directly after the fermentation is complete, eliminating their separation from the production medium as well as the bacterial cultures used, i.e. both dead and living cells of said mutants by separation on filters and centrifuges. Furthermore, their possible purification, concentration by concentration and stabilization are eliminated. Thus, in addition to the effect of alkaline proteases, biomass of production microbes and nutrients from the culture liquid are used for feeding purposes.

However, a liquid or dried protease preparation may be used for feeding purposes.

In particular, the increased technical effect of the first method is that, in addition to the action of proteases, in particular alkaline, to break down nutrients and thereby increase feed conversion, biomass of producing microbes and nutrients from the culture liquid are used without the risk of mycotoxins. In addition, simplification of the preparation technology is advantageous as separation, concentration and purification are eliminated.

In the second method, the technical effect is increased in that, in addition to the digestion of nutrients and the increase in feed conversion, the concentrated protease preparation, mainly alkaline, is easily transportable to the feed mixtures and is easier to handle.

EXAMPLE 1

Protease enzyme is added to the broiler chickens feed mixture with 2% culture liquid,

Example 2

The separated and concentrated enzyme protease preparation is added to the laying compound feed in an amount of 1%.

EXAMPLE 3

Enzyme protease and culture liquid are added to the pig feed mixture in an amount of 1.5%.

Claims (3)

SUBJECT A livestock feed composition enriched in proteases, in particular alkaline, characterized in that it contains enzymes produced by mutants of Bacillus coagulains strains from Lactoflora Nos. 476 and 477 and Bacillus cereus from Lactoflora Nos. 478 and 479. 2. A process according to claim 1, wherein the proteases are added to the feed mixture together with a culture liquid containing these enzymes. 3. A process according to claim 1 wherein the proteases are added to the feed mixture after separation from the culture liquid, optionally after concentration, purification and stabilization.