CS237777B1 - Processing of riboflavine - Google Patents
Processing of riboflavine Download PDFInfo
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- CS237777B1 CS237777B1 CS816727A CS672781A CS237777B1 CS 237777 B1 CS237777 B1 CS 237777B1 CS 816727 A CS816727 A CS 816727A CS 672781 A CS672781 A CS 672781A CS 237777 B1 CS237777 B1 CS 237777B1
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- yeast
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- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 title claims 5
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 title claims 3
- 229960002477 riboflavin Drugs 0.000 title claims 3
- 235000019192 riboflavin Nutrition 0.000 title claims 3
- 239000002151 riboflavin Substances 0.000 title claims 3
- FCASKLHVRFDIJB-UHFFFAOYSA-N Riboflavine Natural products Cc1cc2N=C3C(NC(=O)NC3=O)N(CC(O)C(O)C(O)CO)c2cc1C FCASKLHVRFDIJB-UHFFFAOYSA-N 0.000 title 1
- 244000063299 Bacillus subtilis Species 0.000 claims description 9
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 2
- 229910052799 carbon Inorganic materials 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 2
- 235000015097 nutrients Nutrition 0.000 claims 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 239000002028 Biomass Substances 0.000 claims 1
- 238000005273 aeration Methods 0.000 claims 1
- 229940041514 candida albicans extract Drugs 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 229910052500 inorganic mineral Inorganic materials 0.000 claims 1
- 239000011707 mineral Substances 0.000 claims 1
- 235000013379 molasses Nutrition 0.000 claims 1
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 239000012138 yeast extract Substances 0.000 claims 1
- 229920006235 chlorinated polyethylene elastomer Polymers 0.000 description 4
- 241001465321 Eremothecium Species 0.000 description 2
- 241001553014 Myrsine salicina Species 0.000 description 2
- 238000000136 cloud-point extraction Methods 0.000 description 2
- UGTJLJZQQFGTJD-UHFFFAOYSA-N Carbonylcyanide-3-chlorophenylhydrazone Chemical compound ClC1=CC=CC(NN=C(C#N)C#N)=C1 UGTJLJZQQFGTJD-UHFFFAOYSA-N 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241001669680 Dormitator maculatus Species 0.000 description 1
- 241001585293 Euxoa Species 0.000 description 1
- 240000004120 Syzygium malaccense Species 0.000 description 1
- DCSRPHQBFSYJNN-UHFFFAOYSA-L disodium 4-[(2-arsonophenyl)diazenyl]-3-hydroxynaphthalene-2,7-disulfonate Chemical compound [Na+].[Na+].Oc1c(N=Nc2ccccc2[As](O)(O)=O)c2ccc(cc2cc1S([O-])(=O)=O)S([O-])(=O)=O DCSRPHQBFSYJNN-UHFFFAOYSA-L 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Description
2 03ΟόρβΤΘΗΗΒ OTHOCHTCH Κ ΜΒΚρθ0ΒΟΛΟΓΗΗΘθΚθ3 ΠρΟΜΗΏ-ΛβΗΗΟΟΤΗ, a ΗΜΘΗΗΟ Κ OlIOCOČy ΠΟΛγΗβΗΗΗ ρΒ00φΛ8ΒίϊΗ3 (¾) · ΒΗΤΘΜΗΗ % ΠίίρΟΚΟ ΗΟΠΟΛ£3γθΤΟΗ Β ΟΘΛΒΟΚΟΜ Χθ3Η0ΟΤΒβ,Β OCHOBHOM, Β ΚβΗΗΟΤΒΘ KOpMOBUX flOÓaBOK ΟΘΛΒΟΚΟΧΟ3ΗβθΤ-ΒΘΗΗΗΜ ΜΒΒΟΤΗΗΜ, 9 ΚρΗΟΤ9ΛΛΒΗΘΟΚΗ0 ρΗ0θφ3Ι8ΒΗΗ ΠρΒΜΘΗΗΘΤ-GH Β nWBOÍÍ Β φθρΜΘΠΕΘΒΤΒΊΘΟΚΟβ ΠρΟΜΕΒΠΛΘΗΗΟΟΤΒ. ΡΗόΟφΛΘ-ΒΒΗ nojynamT χβμββθοκημ οποοοΟομ, a Τ8Κ3ΚΘ ΠγΤΘΜ MHKpOÓBO-ΛΟΓΒΗΘΟΚΟΓΟ 0ΗΗΤΘ33. ΗβΒβΟΤβΗ ΟΠΟοΟΟ ΜΗΚροΟΗΟΛΟΓΒΗΘΟΚΟΓΟ npOB3BOJ[CTBaΡΗ0Ο$Λ8ΒΗΗ9 HyTOM ΒΟΠΟΛΒβΟΒΘΗΒΗ KyJIBTypH rpzóa Eremothecium ashbyii Ha COQBO0 φθρΜ9ΗΤ8ΙξΗΟΗΗΟ0 Cpa JIH, COJBPHCameflrajpoji b KyKypy3HHĚ θκοτρβκτ (I). 3a 96 nac nponaccaφβρΜθΗΤβΐίο HaKanJMBaeTOfl ,no 2000 μγ/ji K HejtocuaTKaMTaKoro cnocoóa wiejyHT οτηθοτη τριβτθλι>ηη0 πβρκοΛ ροοτβKyjíhiypa, ΒΟΛΘΛΟΤΒΙϊΘ ΗΘΓΟ yBeJIBHHBaeTCH npOJZOJIXHTQJIÍr-HOCTb φθρΜΘΗΤΘΗΗΒ» Η ϋΟΒΗΙϋβHHyX) HyBCTBBT3JIbH0CTB K 38-ΓΡΗ3ΗΘΗ0Β) ΠΟΟΤΟρΟΗΗθβ ΜΒΚρΟφΛΟροβ. ΗβΒΰΟΛββ 0ΛΒ3ΚΒΜ Π0 ΤΘΧΗΙΠθΟΚΟ0 cyiUHOCTB Β ΛΟΟΤΒ-raeMOMy δφφθκτγ· ΗΒΛΗΘΤΟΗ ΟΠΟΟΟΟ ΠΟΛΡϊΘΗΗΗ ρΒόΟφΛΘΒΠΗβ-BHT8MHH8 $2 ΠΥΤΘΜ KyJIbTHBHpOBaHHH ΘΓΟ ΠρΟ^θΗΤΟΒ-ΜΗΚρΟ-opraHB3MOB BBja Bacillus subtilis Ha ΠΒΤ8ΤΘΛΐ>Ηθδ cpe^Q,coj»psame3 γλ»κο3Η β κθπθοτβθ βοτοηηηκθ yraapojta, ws-HapaABHue coúih b bctohhhkh poctobhx βθοιθοτβ: jpo®seBO0 237777 3 θκοτρθκτ, πθπτοη, rjiyTaMHHOByB καοΛοτγ, npenapara PHICnypHHH Η 3Η81Ι0ΓΗ ΙψρΗΗΟΒ (2). 3a 44 naca nponecca φ6ρ~ΜβΗΤΘΙξΗΗ HSKaiUIUBaJIOCL £0 560 Μγ/λ pHÓO^naBEHa. K flocTomcTBaM saHHoro cnocoóa npoHSBoncTBa Bara-MHHa OTHOCHTCfí BHCOKafl CKOpOCTB ρΟΟΤΘ danouí Η, ΟΛΘβΟΒβ-ΤβΛΒΗΟ, COKpameHHe ΒΰβΜΘΗΗ φβρΜΘΗΤΘΙΙΚΗ.
HesocTaTKaMH cnocoóa ηβτμθτοη cpaBHHTejn>HO mskh!ypoBeas aKTHBHOcra» aocTHraeMHft KyMTypog Bacillussubtilis H HeOÓXOflKMOCTB B flOpOTOCTOBUgaZ Jft ΑθφΗςΚΤΗΗΧΚΟΜΠΟΗβΗΤβΧ φβρΜΘΗΤΘΙίΚΟΗΗΟΐ H pOCTOBOg cpeš;.
Uentio HacToamero Η3θόρθτβΗθ ηβληθτοη ποβηπι6ηηθ βη~xosa pjsóo&aaBKHa.
DbcTaBseHHaH qejn> nocMraeTCfl τθμ, hto b cnocoóeaojiyqeHHH pnóo$flaBHHa, npesycMaTpmaraieM rsyÓHHBoe Kyja-TBBHpoBafíne nposyiiHpyrayix ero MHKpoopraHH3MOB Basa Bacillus subtilis b ycsoBMx aspaw fla nHTaTesBHOfi cpese»coaepxamafi hctohhhk yrseposa, a30Ta, HeoóxosHMue Mane-pam>Hue co jih b npjacyTCTBHH hctohhhkob poctobhx βθηιθοτβ»b Ka^iecTBe nponynHpyíoiwx ρκόοφυΒΒΗΗ MHKpoopraiiHSMOB asBHSa Bacili US subtilis HCflOJEbSyiOT ΙΠΤΘΜΜ Bacillussubtilis BffifflreHeTHKa-304 zjiz BHHMreHeTKKa-304a. ΙΊρκ stom KyjcbTHpoBaHJae Bexyr Ha nHTaTej&Hož cpese,cosepKamefi b KanecTB© ηοτοηκπκβ yrflepo.ua caxap-cupenhah Mejiaccy, a β κβηθοτβθ hctohhhkob poctobhx φβκτοροΒHCÍI0JH>3yK)T óeJEKOBO—BHTaMHHHHfi ΚΟΗΙξβΗΤρΗΤ HJBI ΘΓ0 ΓΚΗρΟ-JIH3aT ΗΛΗ aBTOJIH3aT, ΗΛΗ ΠΗΕΙβΒΗΘ ΙφΟΜΗ, HflH. ΗρΟΚΧΘΒΟΚBKCTpaKT.
McnOJLb3y8MHe ΠΙΤβΜΜΗ—ΟΒβρΧΟΗΗΤβΤΗΚΗ CKOHCTpyHpOBaHHreHeTaraecKHM μθτοαθμη hs η63θβηοημο iDJiyqeHHHX Myramia,Kannan H3 κοτορΗχ hphbohht k 0BepxcHHTe3y βητθμηηθ: rosrrib c-AGR (yCTOtflHBOCTL K pO3eO$JiaBHHy ros37 MyTa-uha b reHe-peryjMTope ribc ycToĚAHBocTB b 8-asaryaHHHy).SaTeM npoBOftHJiacB MyTareHHaa oópaóOTKa HHTposoryaHHHH“HOM, ΗΗΤρ030ΜβΤΟΠΜ0ΉβΒΗΗ03, OÓflyfleHíie yjDt>Tpa$HOfleTOM ZCefleKflHfl aKTBBHHX ΒθρΗΘΗΤΟΒ H3 φβρΜβΗΤΒΒΗΟΚΗΟΪΖ: θρβ£β. 237777
ΠΙτβμμ ΒΗΗΗτθΗθΤΐκ8-304-προτοτροφ, ιπτ3ΜΜ ΒΗΗΗγθηθτη-KSt-304a - ayKCOTpO$’C ΗΘΗ®ΗΤΗφϋΙξΗρθΒ3ΗΗθ2 POCTOBOS ποτ-pedHocTW. 06a raTawa ολκγοοπορογθηηηθ , Ha Bcex cpe®xΚΟΛΚΗΘΟΤΒΟ cnop ΠΟ ορθΒΗΘΗΒΟΟ C HCXOflHHM ΪΙΙΤβΜΜΟΜ ΟΗΒΧΘΗΟHa 3-4 nopofca. ΚνΛΒΤ7Ρ0ΛΒΗΟ-ΜθρφΟΛΟΓΗΗΘΟΚ&Η ΧθρθΚΤθρΗΟΤΗΚΘ ΕΤ8Μ~MOB-npOJiyiíeHTOB ρΗόΟφΛΘΒΗΗΘ Bac.. subtilxs ΒΗΪΒίΓΘΗβ-THKa-304 h BHHHreHeTHKa-304a. ΙΒτθμμ ΒΗΗΗβθηθτηκθ304 ΙΠτβΜΜ ΒΗΗΗρθηθ-Tinta 304a I 2 3
Cnopooópa3y»mas γμμμπimoHica jwhhoS 2-3/t ολομίό jasan ΜορφΟΛΟΓΗΗ ΠΟ,ΖξΜΗΚρΟΟΚΟΠΟΜ ΜορφΟΛΟΓΗΗ Ha pa3-HHX cpejsax MHCO-nenTOHHHfiarap (ΜΠΑ)
Ha 1-2 cyTKH pocTa npa 28-37°C odpa-3yeT κολοηο c nepoBHUM κρββΜ, 3ΒΘ3-jname, .wmbtpom 3-4 mm, ποβθρχηοοτβrjxajQcaa, óJiecmman, hbqt xoJWOBania. KSSSW* ΜΗΗΗΜ3ΛΒΗ3Η Cpe®Cnima23eHa oΓΛΚΚ030β
Poct 603 KanaHHn Ha noBepxHocra6yJU>OHa b βη® wieHo.
He pacTST HaMHHBMaJlbHOa cpa~J© ΦΙΕΗΟΛΟΓΗϊθΟΚΗΘ CBOftCTBa
Ha 2 oyTKH pocta0Ópa3yQT KOJIOHMJtttaMBtpOM 2-3 MM,ηρΚΟ-ΗΘΛΤΟΓΟ HB0Ta,
MoypHcnapyiOT by^-nynax χθλτημUB6T0M, KOJIOHHHspyrnue c raajWHMKpaeM, óxecTHiHHe Ρβοτβτ npa 20-42°C, onTHMyM 37°CpH 5,0-9,0, onTHMyM 7,0-8,0. Υγλθβολη: xoporao yTHJiH3HpyK)T rJnoKOsy,caxaoo3v. KravMa-n.
Cmapra: yTiUB3Hpy»T θτθηολ, copčutoji.Hctohhhkh aeoTa: xoporao ycBaHBaeTΗΜΜΟΗϋϋ, MOH9BítHy, ΗΗΤρβΤΗ.
SteJiatHHy pasMHxaet. 237777 5
Cnocoó ocyniecTMíiOT cjiejjyiomKM oópa3ow: KyjiBTypyEIHOÍreKeTiiKa-304 hjih BHMlreHeTiiKa-304a· BupatHiiBaioT Haarapíi3OBaHHOií cpcjje XoTTHHrepa hjih μηοο-πθπτοηηομ arape(ί.Ώ,ΙΑ) b TeneHJae 1-2 cyTOK npn TeMnepaType 28-37°C. SaTewcycneH3Z!3 ιυιβτοκ nepeAaioT b kojtóu c noceBHoii cpejioíi,coxepeinnieii wejiaccy, óejiKOBo-BHTaMHHHUíí κοκπρΗτρητ (EBK),κ veine pajm-iue cojih, jihóo b KapTodjejiLiiyK noceBHyio cpe^y,jiiíóo b LEE. IIoceBHoii waTepnaji BupainHBaior b Teneiíiie17-24 nacoB na Kanajnce 200 có/mhh. Saiew noceBHoii oe-pnan nepe;;aijr b KOJn-raecTBe 5-10$ b ocHOBiiyw (pepMeirra-HiiOHHyiO cpeny. Epopecc (pepMeiiTamiH ocymecTBJíHOT b kojtóhxcpjieHMeiiepa na KanajiKe, jihóo b mepMeHTepe πρι ηητθηοκβηομnepe^einiiBaHirUi κ aspano b Tenemie 46-50 nacoB npn TeMne-paType 37°C, pH 6,5-7,5 (b Hanajie φθρΜβΗΤΗφίΗ) Ha nHTa-TexLHoii cpejie, coxepxaqeH hctohhhkh yrjiepojia, HanpHMeprjujKOsy, caxap03y, caxap-cupen, Mejiaccy, KpaxMajr; hctoh-HKXK. ΜΕΚβρΟΛΒΗΟΓΟ a3OTa, HanpHMep CO JIH ΗΜΜΟΗΗΗ, ΜΟΗΘΒΗ-Hy.EHrpaTu; cojih mocípopa, HanpHMep ňocfeTa KajiHH, awMo-HHH h jipyrne cojih; cojih Mn++0^012, linSO^) , a TaraieKCTOHHHKH pOCTOBHX IjaKTOpOB, HanpHMep ÓHOMaecy KOpi.lOBO-ro j-poxxeBoro óejnca (EBK), rH£pojiH3ara z amojinsaTn EBK,nmeBne jjpoxxn, ApoxxeBoii θκοτρηκτ, KyKypysnya a coeBy»Myny, i<yKypy3Huií οκοτρηκτ, nwpojiH3aT κη3θηηη, πβπτοη.'lepe3 46-50 nacon φβρΜβΗΤΒΐΐΗΗ b KyjiBTypaiiBHon xhjikocthKaKaiuiHBacTCH 700-1000 μγ/ji pHóopJiaBEHa.
Cr.ocoó HJíJiacTpnpyeTCH npiaiepaj.iH: IIpiMep I.
OjiKocyTOHKyio KyjiBTypy Baciilus subtilis BHHMre-ηοϊπκη-3Ο4, BuparieHHyio na κοοπκαχ MHA, nepeceBaioT naζπχ,κριο noceBHyio cpeuy, coxcp-r.cunyio 6$ Mejiaccu, 1$ EBK,π MHHepajiBHHe cojih. IIoceBHaH cpej;a γοτοβπτοη pa3BejjeHH-e-M nonojiau CTepmiBiioiÍ bojioh ωθρΜβΗτειυιοΗΗοϋ cpej3.ii, coc-•ra3 κοτοροκ npHBeAeH Híixe. BupainiiBaHHe ποοθβηογο MaTe-pnana npoBOňHT b KOJióax OpjieHMefíepa, oóiewoM 750 mx(oótew cpejju 25-100 mji), Ha Kanajiice 200 oó/mhh πρκ 37°C237777 Β τβΉβΗΗβ 17 nacoB η nepeAaiOT b KOjiireecTBe 10$ b φθρΜβΗ-TaiyioHHyio cpejty cjieflyamero cocTaBa:
Mejiacca 12$ BBK 2$ (NH4)2S04 · 0,2$ e2hpo4 _ · 1,4$ EÍ2PO4 0,6$ I'.!gS04 ‘ ; 0,01$ íía^CgHjO? . 0,1$ pH 7,0-7,2. (iíirrpaT HaTpiís) í&epMeHTamGQ ocymecTBJíaioT b KOJióax SpjieHMeiiepa,cÓwmcm 750 nui (oóteM cpeAU 25 mji) Ha KaqajiKe 200 oó/míihnpa 37°C. ^epes 48 ebcob φβρΜβΗΤΒΐίΚΗ KyjiBTypajiBHaH jkíja-koctb coAepziíT 700-1000 μγ/ji piíóo^jiaBHHa.
Hpozep 2. Ακελογιπθη npimiepy Ι,τολβκοBacillus sďbtilis -304a.
HCnOAL3yjOT IQTHMM
Hpiaiep 3. B KanecTBe npoAyiíeHTa Taicxe ncnojn>3yiOT uiTajvwBIH'Il'IreHeTiiKa-304. šepMeHTaiiíioHHaa cpe^a ii ycjioBiffi ωθρ- i.:eK?aiíiuí xaK yxasaHO b npinviepe I. OTJiíraiíeM HBjíneTCH coc-T£3 noceBHoii cpeAH, KOTopaa γοτοβητοη cjie^yw-ai oópa3OM:καρτοφΰΑΒ oHHinam ot KOKypu, pe.ayT na Kycmi π 3ajiEBaiorKéúOJIBUiíIM KOjnneCTBOM BOAH, IlOCJie ΗΘΓΟ CTepHJIH3yK)T ΠρίΙ0,8-1 htm. rioceBEyio cpejiy saceEaiOT c kochkob ΜΠΑ, Kyju>-typa pacTeT b koaóox Ha 750 mji (oÓseM cpe au 50-100 mji)na Ka^&EKe npa 37°C b TencHiíe 24 -íacoB. &epMeHTaunoHHyiocpe^y 3aceEaOT 10$ ποοθβηογο MaTepiiajia, d;epMeHTaii,Eo npo-bo£h? Kai< b npmepe I. TTepe3 48 nacoB cpe ptie Hraním KyjiLTypajibHafl wkoctbcoAepKHT 700-1000 μγ/λ pnóociuiaBííHa.
Hpmaep 4.
To se κηκ b npuMepe 3, tojibko ncnojn>3yioT nrraMM Bacillas subtilis -3Ó4a. 237777 7 llpiaiep 5.
KyjiBTypa, nocesnaa cpejia, ycjioBHH ctepMeHTapra kecb npuMepax 1,2 h 3. Otjihhhom ηβληθτοη cocTaB $epMeHTa-η,ηοηηοη cpeAH, γλθ: caxapo3a (hjih caxap-CKperO 12-14% EBK 3-4% MíuiepajiiHtie cojih xan b npiaiepe I. ^epes 48 aacoB ftepMeHTapmí KyjiiTypajitHan jkerkoctlcορρρ&πτ 700-1000 wi/n pHÓopwiaBHHa.
Hpiaiep 6.
OjpiocyroHKyio KyjiLTypy uiTawMa BHMMreueTHKa-304 ckochkob LOLA nepeiíocaT b noceBiiyio cpe^y 1.·0ΙΒ, Ha κοτοροϋc-ΣΟ nacoB na xanajiKe npn 37°C γοτοβητοη noceBHofi waTe-pnaji. 3 KO.iiwecTBe 10% ποοθβηοη MaTepnazt nepenocHTčH bpepr.ieHTamíOHHyio cpe^y, iaieioqyio cocTaB: rjnoK03a 8%
ApoJXseBoíi 3KCTpaKi 0,5% rEApojiií3aT xa3 e jma 0,02% (l-lOSHO ΒΒΜΘΗΗΤΒ nen-tohom) yjiHepajiBHue cojih kok b npHMepe IpH 7,1-7,2 šepMeKTaiiHfi wihtch 44-50 aacoB npn TewnepaType 37°C bKOJióax Ha KaaajiES 200 oó/mhh. %pe3 48 aacoB áep.veHTaiíHH KyjisTypajiiHaa sh^koctbco^epsHT 700-1000 μγ/ji pHÓoJuiaBEHa.
KaKcrawiBHBa βηχολ pnóoíTviaBHHa b npejyiaraeMOMc~ccoóe cocTaBJíaeT 1000 μγ/ji sa 48 aacoB pocTa KyjiBTypuna cHHTeTHHecKHx kjih κομμθκοηηχ cpejiax, hto Ha 40%HpCBOCXO/UK? aXTHBHOCTB ΕΤΗΜΜΟΒ-ΠρΟ^ΗΘΗΤΟΒ B ΠρΟΤΟΤΗΠΘ.KpoMe Toro, b oTJiEHne ot yKa3aHHoro npoTOTima, Hcnojitsy-Kzr 5oji.ee προοτκβ cpep.u, ne co^epsainHe noporocToaupíx komΗΟΗΘΗΤΟΒ.
HojiyHeHiie piióo^JiaBHna npejyiaraeMHM cnocoóoi.i odjta-žiae? pajioM npeiaiyneciB ho cpaBHeHHio c jieíicTByniyíMH bHacToamee Bpewa npoHSBOflCTBOM pHóoďxnaBHHa c πομοεβιο 37777 acKOKíméTa Eremothecium ashbyii (óa3OBUM tjnocoóOM).Πομεμο y:se ynowiiHaBiiiGíicH'mskbiiioíí TpeóOBaTejihHOCTH κcocTasy πιιτ^τολβηβχ cpejt, b npejyiaraeKOM cnocoóe ποητκB£Boe coKpaqaeTCH Bpei-ra $epMeHTau,XH (48 nacoB Β»Λθοτο72-96 nacos) , a Taoce yMCHBinaeTcn bo3MOhíhoctb 3arptfi3He-ηκπ KyjiBTypu nocTopoiiiieii Lwpo^jjiopox. ÍIo cpaBneniBO c προτοτιιποΜ npejyiaracMUii cnocoó xa-xaKTepxsyeTca óoxee becokxm ypoBneM Buxojxa npoAyxTa κbosmokmoctbk» EcnojiB3OBaTB npocrue h .nocTynnue ΠΟ COCTaBycpe£ii. 237777 ΦΟΗ/ΓΛΊΑ ΜΞΟΕΡΕΤΕΗΜΗ . 1, Cnocoó nojiynenna piíúcůJi&siaia nyreM rnyÓHHHoroKyjILTIIBlípOBaHUH npOAYUJWWK ero MiffipOOpraHH3MOB BflJTaSccillus subtilis b ycjiosHHX aspaiwi aa πΗτητεχΒΗοίί cpe-He, coneplamen hctohhhk yrneposa, aaora, neoóxoAEr.ttie mh-ΗΟρΠΠΒΠΚδ COJ1H S HpHCyTCTEHH nCTOHHKKOB pOCTOBHX BeiUeCTB,oTHircawHicn τθμ, hto, c ηθλβώ ποβηιιιθηκη saxo^a pnóo-cpxasnHa, B^KanecTse nponynnpyiaíiiKx pnóoňuiaBKH Miccpoopra-ΚΚΞί.ΙΟΞ ÍÍ3 Eippa Bacillus subtilis HCnOJIB3yKIT UITSMM Bacil-lus subtilis hffi2LreHerni:a-304 nan BIEWeHeTHKa-304a. 2. Cnocoó no n.I, oTJUMaioiiíníícfl τθμ, n?o KyjiLTnsn-poLanxe Bepyi Ha Πίΐτ&τβιΐΒΗ0Μ cpe^e, conep:aanen b xanecT-Be HCTOHHzna yrjiepo^a caxap-cupen min Mexaccy, a b nanecT3e HCTOHHKKOB pOCTOBUX ΟΠΚΤΟρΟΒ ÍICnOJIB3yíOT óejIKOBO-Bnra-ιηηιηηΰ χοηρεητρητ xwi ero rnjipojinsaT nxn aBTOJiH3aT, minnmueBHe Apozxn, min uposseBoii θκοτρπκτ. IICTOHHHKK ηΗφορΜΒΙΤΗΠ, npHHHTUe BO BHHMaHHe npn3xcnepTH3e: 1. ΑΒΤοροκοβ CBWTejiBCTBo CCCP Jé 389132, mi. CI2 3 5/04, 1969. 2. Πητθκτ OHIA Jé 3900368, κλ.195-96, onyójihk. 1976.*- 237777 10 AHHOTAm-ia npeAJiaraeMutí enocoó nojiyneHiM pxóopjiaBHHa ocHOBanna κοπολη3oBaHHii b KaaecTBs npoAypHpyioiiBix ero Μκκροορ-raHKSMOB BiTaMMOB Bacillus subtilis BHI'GIreHeTíma-304kjík BífiLíreKeTiíKa-304a, nojiyHeiainx iis po^KrejihCKoro uitom-wa, ne oÓJiaAaiomero CBepxcnHTe30M pKóoiuiaBKHa. LlaKC^ajiB-ΗΚΠ euxoa pjtóoěnaBHHa b npejuaraeidowi cnocoóe cocTa&jweTI00C ’.τ/'λ 3a 48 Kacos pocTa. Ho cpaBHeHíW c mctoaom no-ΛϊκβΗΕΗ pKÓO(J.viaBKiía Ha ocHOBe KyxBTypu rpnóa Erenothcci-•j.Ei i shbyii , npe^jiaracMEH cnocoó no3noxjieT ηοκτκ BpBoecoxpaah?b Bpei,K φθρΜβίΐτεαίϋΗ, κροι.ίΰ Toro.iicnojiBsyeTCflóoxse npoc?oe h ρ,οοτγπΗοο cupne pjia cpe^, h yMeHBuiaeTcn2O3:.:o.'-tkoctb 3arpn3HeKiw Kyji&T.ypu nocTopoHHež Μηκροφηοροίί.Ho CpOBHOHJQ C ΠρΟΤΟΤΠΠΟΜ (ΠΗΤ6ΗΤ CUiA Jí> 3.900.368) , ΓΛθKcncHB3yeTCK xyjiBTypa Bacillus subtilis , npejmaraeMbiiicnocoó Ha 40% yBejiXKHBaeT ehxoa pnóo$JiaBHHa κ nosBOJineTκοπολβ3Οβητβ προοτκθ οιίΗτετκΗβοκΗΘ ΜΗ KOMiuieKCHue cpe-P.U, He CO£ep:saiHIie AOporOCTQHllUÍX KJIH ΑβφΗΗΗΤΗΗΧ ΚΟΙΛΠΟΗβΗ-TOB. 237777
2 03ΟόρβΤΘΗΗΒ OTHOCHTCH Κ ΜΒΚρθ0ΒΟΛΟΓΗΗΘθΚθ3 ΠρΟΜΗΏ-ΛβΗΗΟΟΤΗ and ΗΜΘΗΗΟ Κ OlIOCOČy ΠΟΛγΗβΗΗΗ ρΒ00φΛ8ΒίϊΗ3 (¾) · ΒΗΤΘΜΗΗ% ΠίίρΟΚΟ ΗΟΠΟΛ £ 3γθΤΟΗ Β ΟΘΛΒΟΚΟΜ Χθ3Η0ΟΤΒβ, Β OCHOBHOM, Β ΚβΗΗΟΤΒΘ KOpMOBUX flOÓaBOK ΟΘΛΒΟΚΟΧΟ3ΗβθΤ-ΒΘΗΗΗΜ ΜΒΒΟΤΗΗΜ 9 ΚρΗΟΤ9ΛΛΒΗΘΟΚΗ0 ρΗ0θφ3Ι8ΒΗΗ ΠρΒΜΘΗΗΘΤ-GH Β nWBOÍÍ Β φθρΜΘΠΕΘΒΤΒΊΘΟΚΟβ ΠρΟΜΕΒΠΛΘΗΗΟΟΤΒ. ΡΗόΟφΛΘ-ΒΒΗ nojynamT χβμββθοκημ οποοοΟομ, and Τ8Κ3ΚΘ ΠγΤΘΜ MHKpOÓBO-ΛΟΓΒΗΘΟΚΟΓΟ 0ΗΗΤΘ33. TβΒβΟΤβΗ ΟΠΟΗΟΛΟΓΒΗΘΟΚΟΓΟοΟΗΟΛΟΓΒΗΘΟΚΟΓΟ ΜΗΚροΟΗΟΛΟΓΒΗΘΟΚΟΓΟ npOB3BOJ [CTBaΡΗ0Ο $ Λ8ΒΗΗ9 HyTOM ΒΟΠΟΛΒβΟΒΘΗΒΗ KyJIBTypH rpzóa Eremothecium ashbyii Ha COQBO0 φθρΜ9ΗΤ8ΙξΗΟΗΗΟ0 Cpa JIH, COJBPHCameflrajpoji b KyKypy3HHĚ θκοτρβκτ (I). 3a nac nponaccaφβρΜθΗΤβΐίο HaKanJMBaeTOfl 96, 2000 but μγ / her HejtocuaTKaMTaKoro cnocoóa wiejyHT οτηθοτη τριβτθλι> ηη0 πβρκοΛ ροοτβKyjíhiypa, ΒΟΛΘΛΟΤΒΙϊΘ ΗΘΓΟ yBeJIBHHBaeTCH npOJZOJIXHTQJIÍr-HOCTb φθρΜΘΗΤΘΗΗΒ »Η ϋΟΒΗΙϋβHHyX) HyBCTBBT3JIbH0CTB K-38 ΓΡΗ3ΗΘΗ0Β) ΠΟΟΤΟρΟΗΗθβ ΜΒΚρΟφΛΟροβ. ΗβΒΰΟΛββ 0ΛΒ3ΚΒΜ Π0 ΤΘΧΗΙΠθΟΚΟ0 cyiUHOCTB Β ΛΟΟΤΒ-raeMOMy δφφθκτγ · ΗΒΛΗΘΤΟΗ ΟΠΟΟΟΟ ΠΟΛΡϊΘΗΗΗ ρΒόΟφΛΘΒΠΗβ-BHT8MHH8 $ 2 ΠΥΤΘΜ KyJIbTHBHpOBaHHH ΘΓΟ ΠρΟ ^ θΗΤΟΒ-ΜΗΚρΟ-opraHB3MOB BBja Bacillus subtilis Ha ΠΒΤ8ΤΘΛΐ> Ηθδ CPE ^ Q NAV »psame3 γλ» κο3Η β κθπθοτβθ βοτοηηηκθ yraapojta, sθapaapaABapaú búohúúúúúúú b b b b b bctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctctct ctct77ctctctctctctctctctct000000 3a 44 naca nponecca φ6ρ ~ ΜβΗΤΘΙξΗΗ HSKaiUIUBaJIOCL £ 0 560 Μγ / λ pHÓO ^ naBEHa. To flocTomcTBaM saHHoro cnocoóa npoHSBoncTBa Bara-MHHa OTHOCHTCfi BHCOKafl CKOpOCTB ρΟΟΤΘ danouí Η, ΟΛΘβΟΒβ-ΤβΛΒΗΟ, COKpameHHe ΒΰβΜΘΗΗ φβρΜΘΗΤΘΙΙΚΗ.
HesocTaTKaMH cnocoóa ηβτμθτοη cpaBHHTejn> HO mskh! YpoBeas aKTHBHOcra »aocTHraeMHft KyMTypog Bacillussubtilis H HeOÓXOflKMOCTB B flOpOTOCTOBUgaZ Jft ΑθφΗςΚΤΗΗΧΚΟΜΠΟΗβΗΤβΧ φβρΜΘΗΤΘΙίΚΟΗΗΟΐ H pOCTOBOg cpeš;
Uentio HacToamero osa3θόρθτβΗθ ηβληθτοη ποβηπι6ηηθ βη ~ xosa pjsóo & aaBKHa.
DbcTaBseHHaH qejn> nocMraeTCfl τθμ, HTO b cnocoóeaojiyqeHHH pnóo $ flaBHHa, npesycMaTpmaraieM rsyÓHHBoe Kyja-TBBHpoBafíne nposyiiHpyrayix ero MHKpoopraHH3MOB Basa Bacillus subtilis b ycsoBMx Aspawa fla nHTaTesBHOfi CPEs »coaepxamafi hctohhhk yrseposa, A30t, HeoóxosHMue Mane-pam> Hue as south b npjacyTCTBHH hctohhhkob poctobhx βθηιθοτβ »B Ka ^ iecTBe nponynHpyíoiwx ρκόοφυΒΒΗΗ MHKpoopraiiHSMOB asBHSa Bacilius US subtilis HCflOJEbSyiOT ΙΠΤΘΜΜ Bacillussubtilis BffifflreHeTHKa-304 from BHHMreHeTKKa-304a. ΙΊρκ one hundred meters KyjcbTHpoBaHJae Bexyr Ha nHTaTej & MDF CPEs, cosepKamefi b KanecTB © ηοτοηκπκβ yrflepo.ua caxap-cupenhah Mejiaccy and β κβηθοτβθ hctohhhkob poctobhx φβκτοροΒHCÍI0JH> 3yK) T óeJEKOBO-BHTaMHHHHfi ΚΟΗΙξβΗΤρΗΤ HJBI ΘΓ0 ΓΚΗρΟ-JIH3aT ΗΛΗ aBTOJIH3aT, ΗΛΗ ΠΗΕΙβΒΗΘ ΙφΟΜΗ, HflH . ΗρΟΚΧΘΒΟΚBKCTpaKT.
McnOJLb3y8MHe ΠΙΤβΜΜΗ-ΟΒβρΧΟΗΗΤβΤΗΚΗ CKOHCTpyHpOBaHHreHeTaraecKHM μθτοαθμη hs η63θβηοημο iDJiyqeHHHX Myramia, Kannan H3 κοτορΗχ hphbohht to 0BepxcHHTe3y βητθμηηθ: rosrrib AGR-c (K yCTOtflHBOCTL pO3eO $ JiaBHHy ros37 UHA toll-b-reheat peryjMTope ribc ycToĚAHBocTB asaryaHHHy b-8) .SAT npoBOftHJiacB MyTareHHaa oópaóOTKa HHTposoryaHHHH "HOM, ΗΗΤρ030ΜβΤΟΠΜ0ΉβΒΗΗ03, OflyfleHie yjDt> Tpa $ HOfleTOM ZCefleKflHfl aKTBBHHX ΒθρΗΘΗΤΟΒ H3 φβρΜβΗΤΒΒΗΟΚΗΟΪΖ: θρβ £ β. 237777
-30 Η β 4 4 -30 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 St St St St St St St St St St St St St OC OC OC OC OC OC OC OC OC OC POCTOBOS ποτ-pedHocTW. 06a raTawa ολκγοοπορογθηηηθ, Ha Bcex cpe®xΚΟΛΚΗΘΟΤΒΟ cnop ΠΟ ορθΒΗΘΗΒΟΟ C HCXOflHHM ΪΙΙΤβΜΜΟΜ ΟΗΒΧΘΗΟHa 3-4 nopofca. ΛΒΤνΛΒΤ7Ρ0ΛΒΗΟ-ΜθρφΟΛΟΓΗΗΘΟΚ & ΤΧρρΜΤ MΜΜ M M M M M M M M MOBOBOBOBOBOBOBOBOBOBOBOBOBOB .. .. .. .. .. .. ..tiltiltilHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH 304τθμμ ΒΗΗΗβθηθτηκθ304 ΙΠτβΜΜ ΒΗΗΗρθηθ-Tinta 304a I 2 3
Cnopooópa3y »mas γμμμπimoHica jwhhoS 2-3 / t ολομίό ash ΜορφΟΛΟΓΗΗ ΠΟ, ΖξΜΗΚρΟΟΚΟΠΟΜ ΜορφΟΛΟΓΗΗ Ha pa3-HHX cpejsax MHCO-nenTOHHHfiarap (ΑΠΑ)
Ha 1-2 cyTKH T T n npa 28-37 ° C ο ye ye ye ye ye ye ye ye ye ye ye ye ye ye mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm mm π π π π π π π π. KSSSW * ΜΗΗΗΜ3ΛΒΗ3Η Cpe®Cnima23eHa oΓΛΚΚ030β
Tribute 603 KanaHHn Ha noBepxHocra6yJU> OHa b βη® wieHo.
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Ha 2 oyTKH Honor0Ópa3yQT HAWHMJtttaMBtpOM 2-3 MM, ηρΚΟ-ΗΘΛΤΟΓΟ HB0Ta,
MpHcnapyiOT by ^ -nynax UBθ6λMMM6MMMMM,, OHOHOHOHHHspyrHHHHHHHHKKeeMMMecececececececececec-ec-- ° C, -ececec 37 37 ° CpH 5,0-9,0, onTHMM 7,0-8,0. :Γλθβολη: xoporao yTHJiH3HpyK) T rJnoKOsy, caxaoo3v. KravMa-n.
Cmapra: yTiUB3Hpy »T θτθηολ, copčutoji.Hctohhhkh aeoTa: xoporao ycBaHBaeTΗΜΜΟΗϋϋ, MOH9BítHy, ΗΗΤρβΤΗ.
SteJiatHHy pasMHxaet. 237777 5
Cnocoó ocyniecTMíiOT cjiejjyiomKM oópa3ow: KyjiBTypyEIHOÍreKeTiiKa-304 hjih BHMlreHeTiiKa-304a · BupatHiiBaioT Haarapíi3OBaHHOií cpcjje XoTTHHrepa hjih μηοο-πθπτοηηομ Arap (ί.Ώ, ΙΑ) b TeneHJae 1-2 cytokine npn TeMnepaType 28-37 ° C. SaTewcycneH3Z! 3 nepυ ioT e T b b b b b b b b b EB EB EB EB EB EB EB EB EB EB EB EB EB EB EB EB EB EB EB EB EB EB EB EB LE LE LE LE LE LE LE LE LE LE LE LE LE LE LE LE LE E E IIoceBHoii waTepnaji BupainHBaior b Teneieiie17-24 nacoB at Kanajnce 200 có / mhh. Saiew noceBHoii oe-pnan NEP ;; aijr b raecTBe wet nurse-5-10 $ b ocHOBiiyw (pepMeirra-HiiOHHyiO cPen. Epopecc (b pepMeiiTamiH ocymecTBJíHOT kojtóhxcpjieHMeiiepa to KanajiKe, south b mepMeHTepe πρι ηητθηοκβηομnepe einiiBaHirUi κ ^ b Aspan Tenemie 46-50 nacoB npn TeMne-paType 37 ° C, pH 6.5-7.5 (b Hanajie φθρΜβΗΤΗφίΗ) Ha nHTa-TexLHoii cpejie, coxepxaqeH hctohhhkh yrjiepojia, HanpHMeprjujKOsy, caxap03y, caxap-cupen, Mejiaccy, KpaxMajr; hctoh-HKXK. CO JIH ΗΜΜΟΗΗΗ, ΜΟΗΘΒΗ-Hy.EHrpaTu; cojih mocípopa, HanpHMep ňocfeTa KajiHH, awMo-HHH h jipyrne cojih; cojih Mn ++ 0 ^ 012, linSO ^); poxxeBoro óejnca (EBK) rH £ pojiH3ara of amojinsaTn EBK, nmeBne jjpoxxn, ApoxxeBoii θκοτρηκτ, and KyKypysnya coeBy »Myny, i <yKypy3Huií οκοτρηκτ, nwpojiH3aT κη3θηηη, πβπτοη.'lepe3 46-50 nacon φβρΜβΗΤΒΐΐΗΗ b KyjiBTypaiiBHon xhjikocthKaKa iuiHBacTCH 700-1000 μγ / μ pHoopJiaBEHa.
Cr.ocoó HJíJiacTpnpyeTCH npiaiepaj.iH: IIpiMep I.
OjiKocyTOHKyio KyjiBTypes of Baciilus subtilis BHHMre-ηοϊπκη-3Ο4, BuparieHHyio on κοοπκαχ MHA, nepeceBaioT naζπχ, κριο noceBHyio cpeuy, coxcp-r.cunyio 6 $ Mejiaccu, 1 $ EBK, π MHHepajiBHHe cojih. IIoceBHaH cpej; and γοτοβπτοη pa3BejjeHH-eM nonojiau CTepmiBiioi oh oh oh oh oh oh oh oh oh oh ii ii ii ii ii ii ii ii ii ii ii ix ix ix ix ix ix ix. BupainiiBaHHe ποοθβηογο If pnana npoBOňHT b KOJióax OpjieHMefíepa, oóiewoM mx 750 (25-100 oótew cpejju np) Ha Kanajiice 200 oo / MHH πρκ 37 C237777 Β τβΉβΗΗβ 17 nacoB η b nepeAaiOT KOjiireecTBe 10 $ b φθρΜβΗ-TaiyioHHyio cpejty cjieflyamero cocTaBa:
Mejiacca 12 $ BBK 2 $ (NH4) 2S04 · 0.2 $ e2hpo4 _ · 1.4 $ Ei2PO4 0.6 $ I '.gS04'; 0.01 $ íía ^ CgHjO? . 0.1 $ pH 7.0-7.2. The pH of the tube is 750 µm (about 25 µmol / cm 2) and 200 µg / cm 2 (37 ° C). ebcob φβρ 48βΗΤΒΐίΚΗ KyjiBTypajiBHaH jkíja-koctb coAepziíT 700-1000 μγ / ji piíóo ^ jiaBHHa.
Hpozep 2. Ακελογιπθη npimiepy Ι, τολβκοBacillus sdbtilis -304a.
HCnOAL3yjOT IQTHMM
Hpiaiep 3. B TecnAtIcE TaicxeNaNtHyTaKa-304. M M pe a oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc oc H H H H H H H 3ajiEBaiorKéúOJIBUiíIM KOjnneCTBOM BOAH, IlOCJie ΗΘΓΟ CTepHJIH3yK) T ΠρίΙ0,8-1 htm. rioceBEyio cpejiy saceEaiOT c kochkob ΜΠΑ, Kyju> -type pacTeT b koaoox Ha 750 mji (oseM cpe au 50-100 mji) to Ka ^ & EKe npa 37 ° C b TencHiíe 24 -íacoB. & epMeHTaunoHHyiocpe ^ y 3aceEaOT 10 $ ποοθβηογο MaTepiiajia, d; epMeHTaii, Eo npo-bo £ h? Kai <b npmepe I. TTepe3 48 nacoB cpe ptie By playing KyjiLTypajibHafl wkoctbcoAepKHT 700-1000 μγ / λ pnóociuiaBííHa.
Hpmaep 4.
This is npuMepe 3, tojbko ncnojn> 3yioT nrraMM Bacillas subtilis -3Ó4a. 237777 7 llpiaiep 5.
KyjiBTypa, nocesnaa cpejia, ycjioBHH ctepMeHTapra kecb npuMepax 1.2 h 3. Otjihhhom ηβληθτοη cocTaB $ epMeHTa-η, ηοηηοη cpeAH, γλθ: caxapo3a (hjih caxap-CKperO 12-14% EBK 3-4% ^ epes 48 aacoB ftepMeHTapmi KyjiiTypajanHan jkerkoctlcορρρ & πτ 700-1000 wi / n pHÓopwiaBHHa.
Hpiaiep 6.
OjpiocyroHKyio KyjiLTypy uiTawMa BHMMreueTHKa-304 OLA Ha OLA OLA · · · · · aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco aco. 3 KO.iiwecTBe 10% ποοθβηοη MaTepnazt nepenocHTčH bpepr.ieHTamíOHHyio cpe ^ y, iaieioqyio cocTaB: rjnoK03a 8%
ApoJXseBoi3KCTpaKi 0.5% rEApre3aT xa3 e jma 0.02% (l-lOSHO ΒΒΜΘΗΗΤΒ nen-tohom) yjiHepajiBHue cojih kok b npHMepe IpH 7,1-7,2 shepMeKTaiiHfi wihtch 44-50 aacoB npn TewnepaType 37 ° C bKOJióax Ha KaaajiES 200 oo / mhh. % pe3 48 aacoB aep.veHTaiiHH KyjisTypaiaHaa sh ^ koctbco ^ epsHT 700-1000 μγ / u pHoJuiaBEHa.
KaKcrawiBHBa βηχολ pnóoíTviaBHHa b npejyiaraeMOMc ~ ccoóe cocTaBJíaeT 1000 μγ / ji with 48 aacoB pocTa KyjiBTypuna cHHTeTHHecKHx kjih κομμθκοηηχ cpejiax, hto Ha 40% HpCBOCXO / UK? aXTHBHOCTB ΕΤΗΜΜΟΒ-ΠρΟ ^ ΗΘΗΤΟΒ B ΠρΟΤΟΤΗΠΘ.KpoMe Toro, b oTJiEHne ot yKa3aHHoro npoTOTima, Hcnojitsy-Kzr 5oji.ee προοτκβ cpep.u, no co ^ epsainHe noporocToaupíx komΗΟΗΘΗΤΟΒ.
HojiyHeHiie piióo ^ JiaBHna npejyiaraeMHM cnocoóoi.i odjta-žiae? pajioM npeiaiyneciB it cpaBHeHHio c jieíicTByniyíMH bHacToamee Bpewa npoHSBOflCTBOM pHóoďxnaBHHa c πομοεβιο 37777 acKOKíméTa Eremothecium ashbyii (óa3OBUM tjnocoóOM) .Πομεμο y: the ynowiiHaBiiiGíicH'mskbiiioíí TpeóOBaTejihHOCTH κcocTasy πιιτ ^ τολβηβχ cpejt b npejyiaraeKOM cnocoóe ποητκB £ Boe-coKpaqaeTCH Bpei epMeHTau $ ra, H ( 48 nacoB Β »Λθοτο72-96 nacos), and Taoce yMCHBinaeTcn bo3MOhíhoctb 3arptfi3He-ηκπ KyjiBTypo nocTopoiiiieii Lwpo ^ jjiopox. IIo cpaBneniBO c προτοτιιποΜ npejyiaracMUii cnocoó xa-xaKTepxsyeTca oxox becokxm ypoBneM Buxojxa npoAyxTa κbosmokmoctbk »EcnojiB3OBaTB npocrue h .nocTynnue ΠΟ COCTaBycpe £ ii. 237777 ΦΟΗ / ΓΛΊΑ ΜΞΟΕΡΕΤΕΗΜΗ. 1 Cnocoó nojiynenna piíúcůJi & shiai Nyree rnyÓHHHoroKyjILTIIBlípOBaHUH npOAYUJWWK ero MiffipOOpraHH3MOB BflJTaSccillus subtilis b ycjiosHHX aspaiwi aa πΗτητεχΒΗοίί CPE-Ile, coneplamen hctohhhk yrneposa, aaora, neoóxoAEr.ttie MH-ΗΟρΠΠΒΠΚδ COJ1H S HpHCyTCTEHH nCTOHHKKOB pOCTOBHX BeiUeCTB, oTHircawHicn τθμ, HTO c ηθλβώ ποβηιιιθηκη Bacillus Bacillus subtilis HCnOJIB3yKIT UITSMM Bacillus subtilis hffi2LreHerni: a-304 nan BIEWeHeTHKa-304a. 2. Cnocoó no nI, oTJUMaioiínionfl τèμ, n'y KyjiLTnsn-poLanxe Bepyi Ha Πίΐτ & τβιΐΒΗ0Μ cpe ^ e, conep: aanen b xanecT-Be HCTOHHzna yrjiepo ^ a caxap-cupen min Mexaccy, ab nanecT3e HCTOHHKKOB pOCTOBUX ΟΠΚΤΟρΟΒ ÍICnOJIB3yíOT ι η ι ι wi wi wi wi wi wi wi wi wi wi wi wi wi wi wi wi wi wi oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz oz. IICTOHHHKK ηΗφορΜΒΙΤΗΠ, npHHHTUe BO BHHMaHHe npn3xcnepTH3e: 1. ΑΒΤοροκοβ CBWTejiBCTBo CCCP I 389132, mi. CI2 3 5/04, 1969. 2. Πητθκτ OHIA Jé 3900368, κλ.195-96, onyójihk. 1976th * - 237 777 10 AHHOTAm ia npeAJiaraeMutí enocoó nojiyneHiM pxóopjiaBHHa ocHOBanna κοπολη3oBaHHii b KaaecTBs npoAypHpyioiiBix ero-Μκκροορ raHKSMOB BiTaMMOB Bacillus subtilis BHI'GIreHeTíma-304kjík BífiLíreKeTiíKa-304a, nojiyHeiainx after IIS-KrejihCKoro uitom-wa not oÓJiaAaiomero CBepxcnHTe30M pKóoiuiaBKHa. LlaKC ^ ajiB-ΗΚΠ euxoa pjtóoěnaBHHa b npejuaraeidowi cnocoóe cocTa & jweTI00C '.τ /' λ 3a 48 Kacos pocTa. Ho cpaBHeHíW c mctoaom no-ΛϊκβΗΕΗ pKÓO (IIa J.viaBKiía ocHOBe KyxBTypu rpnóa Erenothcci- • j.Ei and shbyii, NPE-jiaracMEH cnocoó no3noxjieT ηοκτκ BpBoecoxpaah? B Bpei K φθρΜβίΐτεαίϋΗ, κροι.ίΰ Toro.iicnojiBsyeTCflóoxse NPOC? Oe h ρ , οοτγπΗοο cupne pjia CPE ^ h yMeHBuiaeTcn2O3:.: o .'- tkoctb 3arpn3HeKiw clubs & T.ypu nocTopoHHež Μηκροφηοροίί.Ho CpOBHOHJQ ΠρΟΤΟΤΠΠΟΜ C (ΠΗΤ6ΗΤ CUiA Her> 3,900,368) ΓΛθKcncHB3yeTCK xyjiBTypa Bacillus subtilis npejmaraeMbiiicnocoó Ha 40% yBejiXKHBaeT ehxoa pnóo $ JiaBHHa κ nosBOJineTκοπολβ3Οβητβ προοτκθ οιίΗτετκΗβοκΗΘ ΜΗ KOMiuieKCHue cpe-PU, He CO £ ep: saiHIie AOporOCTQHllUÍX KJIH ΑβφΗΗΗΤΗΗΧ ΚΟΙΛΠΟΗβΗ-TOB.
Claims (2)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU802981423A SU908092A1 (en) | 1980-09-04 | 1980-09-04 | Method of obtaining riboflavin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS672781A1 CS672781A1 (en) | 1984-11-19 |
| CS237777B1 true CS237777B1 (en) | 1985-10-16 |
Family
ID=20917508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS816727A CS237777B1 (en) | 1980-09-04 | 1981-09-11 | Processing of riboflavine |
Country Status (4)
| Country | Link |
|---|---|
| CS (1) | CS237777B1 (en) |
| HU (1) | HU185652B (en) |
| SU (1) | SU908092A1 (en) |
| YU (1) | YU210281A (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3940071A1 (en) | 2020-07-15 | 2022-01-19 | Chifeng Pharmaceutical Co., Ltd | 5-methylfolate producing microorganism |
| US20230272442A1 (en) | 2020-07-15 | 2023-08-31 | Chifeng Pharmaceutical Co., Ltd. | 5-methylfolate producing microorganism |
| EP4006139A1 (en) | 2020-11-26 | 2022-06-01 | Acies Bio d.o.o. | Genetically engineered bacterium capable of producing cytokinins with isoprenoid side chains |
-
1980
- 1980-09-04 SU SU802981423A patent/SU908092A1/en active
-
1981
- 1981-09-01 YU YU210281A patent/YU210281A/en unknown
- 1981-09-04 HU HU256281A patent/HU185652B/en unknown
- 1981-09-11 CS CS816727A patent/CS237777B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| SU908092A1 (en) | 1984-12-30 |
| CS672781A1 (en) | 1984-11-19 |
| HU185652B (en) | 1985-03-28 |
| YU210281A (en) | 1983-06-30 |
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