CS238463B1 - Metal snouth moth(pyralis farinalis)prevention agent for cereals - Google Patents

Abstract

The invention relates to a composition for protecting cereals against powdery mildew. The essence of the invention lies in the fact that the composition contains as an active substance a 0.0025 to 0.05% by weight solution of trans-6-(4-hydroxy-3-methylbut-2-enyl)-aminopurine or a 0.004 to 0.05% by weight solution of trans-6-(4-hydroxy-5-methylbut-2-enyl)-aminopurine rhinoside. The invention has application in agriculture.

Description

The invention relates to a composition for the protection of cereals against powdery mildew.

Under European conditions, cereals are more vulnerable to disease than pests. The harmfulness of powdery mildew, Erysiphe graminis DC ·, has increased substantially in recent years. The disease is more aggressive and is no longer a problem only in barley, but has become equally dangerous in wheat and oats. Early and strong infestation reduces the life of plants that can also die, reduces the number of roots and the number of fertile offshoots, reduces the number of grains in the ear and the grain size »Later or weakly infested powdery mildew is negatively affected adversely affecting their hibernation e / Ď. H · Brooks: Ann · Appl. Biol. 70, 2: 149 (1972). Variety resistance and tolerance affect the height of the losses.

Actual losses are often determined by the degree of attack of plants at a particular growth stage. According to Kols, the percentage of losses is proportional to the product of the coefficient of 2.5 and the square root of the percent attack on the upper three sheets / A. Obst: Bayer. landw. B. 52, 6: 702 (1975)]. The losses caused by powdery mildew on wheat were estimated at 10 to 10 in the German Democratic Republic. 15%. In Yugoslavia, the amount of damage caused by powdery mildew was 4 to 16% and in some cases up to 45% / B. S. Kostic, T. T. Hristina: Savr. Poljoprivr. 14, 779 (1966). Abroad, systemic fungicides such as 2-ethylamino-4-hydroxy-5-n are applied against powdery mildew. butyl-6-methylpyrimidine, N-tridecyl-2,6-dimethylmorpholine,

1- (N-butylcarbamoyl) -2-methoxycarboxamidobenzimidazole, 1,4-bis- (2,2,2-trichloro-1-formamidoethyl) -piperazine and 1- (4-chlorophenoxy) -3,3-dimethyl-1- / l, 2,4-triazol-l-yl / butan-2-one. If these fungicides are applied preventively, the protection period is several weeks.

N-Tridecyl-2,6-dimethylmorpholine works curatively up to 10 days after inoculation / li. Scheinpillug, V. Paul, P. Kraus: Mitt.

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Bundesanst. Land - Foratwirt. 178: 178 (1977) //. Upon application of these fungicides, the pathogen, Erysiphe graminis, is partially inhibited but forms sporulating organs. No systemic inhibition of pathogen and therefore destruction of the pathogen · Systemic fungicides have the disadvantage of phytotoxicity, which may not manifest themselves as corrosive but manifest as yield depression, in addition they are substances toxic to humans, animals and beneficial insects and therefore strict safety regulations.

The aforementioned drawbacks are substantially eliminated by the cereal preservative according to the invention, which comprises from 0.0025 to 0.03% by weight of active ingredient. trans -6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine solution or 0.004 to 0.05% w / w solution of trans -6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine riboside .

The advantages of the proposed cereal powdery mildew composition over the prior art are that the present composition is more effective and healthy when using trans-6- (4-hydroxy-3-methylbut-2-enyl) -aninopurine and its riboside alone or because they are naturally present in every plant. Finally, they were found to have anti-tumor effects / G. B. Chheda, A. Mittelman, Biochem Pharmacol. 21, 27/1972 // · Combined with industrial organic fungicides that are effective against fungal diseases, their toxicity is reduced compared to conventional combination spraying ·

Example 1

One hundred plants of highly susceptible spring barley cultivator on powdery mildew (Slovak Danube Market) with first to second leaves developed are sprayed with 50 ml of a 0.006% by weight aqueous solution of trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine. The plants are then infected with conidia of the fungus Erysiphe graminis DC. After 15 days, the growth of powdery mildew on the leaves was evaluated by visual, light and raster microscopy. Growth of said fungus was completely inhibited * Conidia of fungi were 95% germinated and 87% plasma-lysed · Example 2

The procedure was as in Example 1 except that 0.03 wt. aqueous trans-6- (4-hydroxy-3-methylbut-2-yl) -aminopurine. The growth of said fungus was completely inhibited. Conidia fungi were 10% non-germinated and 95% plasma-lysed ·

Example 3

238 463

Leaf segments of highly susceptible spring barley cultivator on powdery mildew (Slovak Danube market) are incubated in spring with 4 ml of an aqueous solution of 0.0025% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine for 24 hours . Then the leaf segments are infected with conidia of the fungus Erysiphe gratuinia DC. After 15 days, powdery mildew growth per leaf and leaf segment was assessed by visual, light and scanning microscopy. The growth of said fungus was completely inhibited. Conidia fungi were 100% germinated and 90% plasma-lysed. Example 4

The procedure was as in Example 3 except that 4 ml of an aqueous solution containing 0.0025% trans-6- (4-hydroxy-3-methylbut-2-enyl) aminopurine and copper oxychloride was used. The growth of said fungus is completely inhibited as in Example 3 ·

Example 5

The procedure was as in Example 3 except that 4 ml of an aqueous solution containing 0.0025% trans-6- (4-3-methylbut-2-enyl) -aminopurine and sulfur was used. The growth of said fungus is completely inhibited as in Example 3.

Example 6

The procedure is as in Example 3 except that 4 ml of an aqueous solution containing 0.0025% trans-6 * (4-hydroxy-3-methylbut-2-enyl) -aminopurine α1,2-bis- (3) are used. -metoxykarbonyltioureido / benzene. The growth of said fungus is completely inhibited as in Example 3 ·

Example 1

The procedure is as in Example 3 except that 4 ml of an aqueous solution containing 0.0025% trans-6- (4-hydroxy-3-methylbut-2-enyl) -arainopurine and ethylene-1,2-bis- Manganese dithiocarbamidate. The growth of said fungus is completely inhibited as in Example 3.

* · »

Example 8

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The procedure is as in Example 3 except that 4 ml of an aqueous solution containing 0.0025% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine and zinc ethylene-1,2-bisdithiocarbamidate is used. . The growth of said fungus is completely inhibited as in Example 3 ·

Example 9

The procedure is as in Example 3 except that 4 ml of an aqueous solution containing 0.0025% trans-6- (4-hydroxy-3-methylbut-2-thenyl) -aminopurine and N, N-dimethyl-N is used. -dichlorofluoromethylthio-N '-phenylsulfamide · The growth of said fungus is completely inhibited as in Example 3 »

Example 10

The procedure is as in Example 3 except that 4 ml of an aqueous solution containing 0.0025% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminapurine and 3- (3,5-oxazolidine) is used. -2,4-diol. The growth of said fungus is completely inhibited as in Example 3.

Example 11

The procedure is as in Example 3 except that 4 ml of an aqueous solution containing 0.0025% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine and 2,4-dinitro-6- is used. -octylphenylcrotonate mixture of 2 *, 3 *, 4'-isomers · Growth of said fungus is completely inhibited as in Example 3.

Example 12

The procedure is as in Example 3 except that 4 ml of an aqueous solution containing 0.0025% trans-6- (4-hydroxy-3-methylbut-2-enyl) aminopurine and 0,0-dimethyl-O- is used. (3-methyl-4'-nitrophenyl) -thiophosphate · Growth of said fungus is completely inhibited as in Example 3 ·

Example 13

The procedure is as in Example 3 except that it is. use 4 ml of an aqueous solution containing 0,0025% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine and 0,0-dimethyl238 463

- / 2,2,2-trichloro-l-hydroxy / -etylfosXonát. The growth of said fungus is completely inhibited as in Example 3.

Example 14

The procedure is as in Example 3 except that 4 ml of an aqueous solution containing 0.0025% trans-6- (4'-hydroxy-3-methylbut-2-enyl) aminopurine and 2-dimethylamino »5.6 is used. -dimethylpyrimidin4 * -yl-N, N-dimethylcarbamate · The growth of said fungus is completely inhibited as in Example 3.

Example 15

The leaf segments of the spring susceptible cultivar of powdery mildew (Slovak Danube market) are incubated in the autumn period in 4 ml of an aqueous solution of 0.005% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aininopurine for 24 hours. . Then, the leaf segments are initiated with conidia of the fungus Erysiphe graminis DC. After 15 days, powdery mildew growth on the leaf segments was evaluated visually by light and scanning microscopy. The growth of said fungus was completely inhibited. Conidia fungi were 100% germinated and 90% plasma-lysed. Example 16

The procedure is as in Example 15 except that 4 ml of an aqueous solution of 0.005% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine and copper oxychloride are used. The growth of said fungus is completely inhibited as in Example 15.

Example 17

The procedure was as in Example 15 except that 4 ml of an aqueous solution of 0.005% trans-6- (4-hydroxy-3-methylbut-2-enyl) aminopurine and sulfur was used. The growth of said fungus is completely inhibited as in Example 15.

Example 18

The procedure is as in Example 15 except that 4 ml of an aqueous solution of 0.005% trans-6- (4-hydroxy-3'-methylbut-2-enyl) aminopurine α1,2-bis- (3-methoxycarbonylthioureido) is used. benzene. The bastion of said fungus is completely inhibited as in Example 15 ·

Example 19 238 463

The procedure is as in Example 15 except that 4 ml of an aqueous solution of 0.005% trans-6- [4-hydroxy-3-methylbut-2-enylamino] aminopurine and manganese ethylene-1,2-bis-dithiocarbamidate are used. is completely inhibited as in Example 15.

Example 20

The procedure is as in Example 15 except that 4 ml of an aqueous solution of 0.005% trans-6/4-hyúroxy-3-methyl-but-2-aminopurine and ^the enyJp ethylene-l, 2-bis-ditiokarbamidan zinc. The growth of said fungus is completely inhibited as in Example 15 ·

Example 21

The procedure is as in Example 15 except that 4 ml of an aqueous solution of 0.005% trans-6- (4-hydroxy-3-methyl-but-2-enyl) -aminopurine and N, N-dimethyl-N ^, -dichlorofluoromethylthio- N * -fenylsulfamid. The growth of said fungus is completely inhibited as in Example 15 ·

Example 22 »

The procedure of Example 15 is followed except that 4 ml of an aqueous solution of 0.005% trans-6- (4-hydroxy-3-methylbut-2-enyl) aminopurine and 3- (3,5-dichlorophenyl) -5-methyl-5 are used. vinyl-l, 3-oxazolidine-2,4-dione. The growth of said fungus is completely inhibited as; in example 15 ·

Example 23

The procedure is as in Example 15 except that 4 ml of an aqueous solution of 0.005% trans-6- (4-hydroxy-3-methylbut-2-enyl) aminopurine and 2,4-dinitro-6-octylphenyl crotonate mixture 2 'are used. The growth of said fungus is completely inhibited as in Example 15 ·

Example 24

The procedure is as in Example 15 except that 4 ml of an aqueous solution of 0.005% trans-6- (4-hydroxy-3-methyl-but-2-enyl) aminopurine and O, O-dimethyl-O- (3-methyl-4-nitrophenyl) are used. / -thiophosphate · Growth of said fungus is completely inhibited as in Example 15 ·

- 7 Example 25

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The procedure is as in Example 15, except that 4 ml of an aqueous solution of 0.005% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aniinopurine and 0,0-dimethyl- [2,2] are used. 2-trichloro-i-hydroxy / -etyltosionát. The growth of said fungus is completely inhibited as; in example 15 ·

Example 20

The procedure is as in Example 15 except that 4 ml of an aqueous solution of 0.005% trans-O- [4-hydroxy-3-methylbut-2-enylamino] aminopurine and 2-dimethylamino-5,6-dimethylpyrimidin-4-yl are used. -N, N-dimethylcarbamate · Growth of said fungus is completely inhibited by ake in Example 15.

Example 27

The procedure is as in Example 15 except that 4 ml of an aqueous solution of 0.005% trans-6- (4-hydroxy-3-methylbut-2-enylamino) aminepurine and a liquid fertilizer are used. The growth of said fungus is completely inhibited by ake in Example 15 ·

Example 28

The procedure was as in Example 3 except that 4 ml of an aqueous solution containing 0.03% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine was used. Growth of said fungus was completely inhibited * Conidia were 100% germinated and 95% plasma-lysed *

Example 29

The procedure is as in Example 3, except that 4 ml of an aqueous solution containing 0.004% trans- (4-hydroxy-3-methylbut-2-enyl) aminopurine riboside is used. The growth of said fungus was completely inhibited as in Example 3. Example 30

The procedure is as in Example 15 except that 4 ml of an aqueous solution containing 0.008% trans-6 (4-hydroxy-3-methylbut-2-enyl) -aminopurine riboside is used. The growth of said fungus was completely inhibited by ake in Example 15 *.

Example 31 238 483

The procedure was as in Example 3 except that 4 ml of an aqueous solution containing 0.05% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine riboside was used. The growth of said fungus was completely inhibited as in Example 3.

Example 32

The wheat leaf segments (cultivar Caucasus) are incubated in 4 ml of an aqueous solution containing 0.0025% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine for 24 hours.

Then. leaf segments infect conidia of Erysiphe graminis DC. After 15 days, powdery mildew growth on leaf segments was evaluated by visual, light and scanning microscopy. The growth of said fungus was completely inhibited. Conidia fungi were 100% germinated and 90% plasma-lysed. Example 33

The procedure was as in Example 32 except that 4 ml of an aqueous solution containing 0.03% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine was used. Growth of said fungus was completely inhibited. Conidia of the fungus were 100% germinated and 95% plasmolyzed.

Example 34

Proceed as in Example 32, except that 4 ml of an aqueous solution containing 0.004% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine riboside was used. example 32.

Example 35

The procedure was as in Example 32 except that 4 ml of an aqueous solution containing 0.05% trans-6- (4-hydroxy-3-methylbut-2-enyl) -aminopurine riboside was used. The growth of said fungus was completely inhibited as in Example 33.

The invention can find wide application in agriculture in the protection of cereals against powdery mildew, by completely inhibiting the growth and development of powdery mildew.

Claims (1)

238 463 A composition for protecting cereal against powdery mildew characterized by the fact that it contains trans-6- (4-hydroxy-3-methylbenz-2-enyl) -aminopurine in the form of 0.0025 & 03 solution or trans-.alpha.-hydroxy-S-methyltourt-2, -enyl / enunopurine riboside in the form of 0.004 to 0.05% by weight solution.